KR101744889B1

KR101744889B1 - Composition for Anti-oxidation, Whitening and Wrinkles protection

Info

Publication number: KR101744889B1
Application number: KR1020150083246A
Authority: KR
Inventors: 안지정
Original assignee: 안지정
Priority date: 2015-06-12
Filing date: 2015-06-12
Publication date: 2017-06-09
Also published as: KR20160146246A

Abstract

The present invention relates to a composition having antioxidative, whitening and wrinkle-reducing effects, and more particularly to a vitamin C, an extract of a herb complex, adenosine, and the herb complex is selected from the group consisting of chamomile, rosemary, lavender, tea tree, Sage and hibiscus.
INDUSTRIAL APPLICABILITY According to the present invention, it is possible to provide an external-application composition having excellent antioxidative, whitening and wrinkle-reducing activities.

Description

[0001] The present invention relates to a composition having antioxidative, whitening and anti-wrinkle effects,

The present invention relates to a composition having antioxidative, whitening and wrinkle-reducing effects, and more particularly, to a composition having excellent antioxidative, whitening and wrinkle-reducing effects by mixing vitamin C, an extract of a herb complex and adenosine, Or a composition having an antioxidative, whitening and wrinkle-reducing effect which is contained in the cosmetic composition as an active ingredient.

The relationship between aging and oxidation is well known through many studies. It is known that wrinkle formation and localized blackening, which are caused by skin aging, are also closely related to these oxidation phenomena. Some people look much younger than their age, and others look older than they really are.

The causes of aging such as skin wrinkle formation and localized blackening are endogenous factors such as age increase, skeletal and muscular shape, genetic factors due to continuous relaxation and contraction of muscles, and environmental pollutants and stress Environmental factors. The skin aging phenomenon caused by various factors described above leads to an increase in wrinkles due to reduction of keratinocyte and fibroblast cleavage, decrease of collagen synthesis, increase of MMP production, increase of signal of melanin generation, and decrease of skin elasticity , And spots, freckles, and black spots are increased.

In particular, recent studies have shown that in the case of healthy skin, free radicals are eliminated by a bio-antioxidant defense factor such as Superoxide Dismutase and Catalase, When it is not used, it is oxidized by active oxygen (a kind of free radicals), and skin cell and tissue functions are deteriorated and it is known that skin wrinkle formation and localized blackening phenomenon are promoted. These reactive oxygen species, which are highly biodegradable, are promoted by external factors (pollution, stress) and internal factors (energy metabolism in the body), and they are combined with lipids to form harmful substances called lipid peroxides. The lipid peroxide acts on blood vessels and causes various diseases including arteriosclerosis and thrombosis. In addition, the collagen production ability which is closely related to the healthy skin is lowered and the abnormal tangling phenomenon of these collagen fibers is increased, and the elasticity of the skin is remarkably lowered. Also, the hyaluronic acid which is responsible for skin moisturization and the glycosaminoglycan It is reported that the production amount is remarkably lowered.

Various methods have been applied to improve the skin aging phenomenon. As a representative method, a method of applying AHA, retinoic acid, retinol, vitamins and the like is mainly used, but there are many controversies about skin irritation and clinical efficacy. It can not be a fundamental countermeasure for oxidation by oxygen. In addition, there are many examples in which a plant extract containing an antioxidant component is used singly, but it is difficult to obtain a satisfactory effect.

As a prior art for improving the skin aging phenomenon, there has been proposed a method of producing a purified product containing Tanshinone I and Tanshinone IIA, which are active ingredients useful for antioxidation, whitening, and wrinkle reduction, from the extracts of Korean Patent No. 10-1513892 A composition for skin regeneration, improvement of wrinkles, antioxidant and skin whitening of Korean Patent Laid-Open No. 10-2015-0019672, antioxidation, wrinkle improvement, anti-acne, anti-inflammation and whitening using Korean green algae of Korean Patent No. 10-0889973 Functional extract having efficacy, a method for producing the same, and a composition for external application for skin whitening and skin whitening comprising an antioxidant component of Korean Patent No. 10-1315325.

However, there were no cases in which the herb complex, vitamin C, and adenosine were used in the preceding prior patents or prior studies.

10-1513892 B1 10-2015-0019672A 10-0889973 B1 10-1315325 B1

Accordingly, it is an object of the present invention to provide a composition comprising an extract of a herbal complex capable of eliminating free radicals, vitamin C and adenosine.

It is also an object of the present invention to provide an excellent cosmetic composition or soap composition having antioxidation, skin wrinkle improvement and skin whitening effect through such a composition.

It is an object of the present invention to include vitamin C, an extract of a herb complex, and adenosine, wherein the herb complex is chamomile, rosemary, lavender, tea tree, cypress, sage and hibiscus.

The extract of the herb complex may be prepared by immersing a mixture of chamomile, rosemary, lavender, tea tree, cypress, sage and hibiscus at 4 to 40 DEG C for 1 to 15 hours in an extraction solvent or extracting at 40 to 100 DEG C Followed by heating and extraction for 2 to 20 hours, followed by concentration.

The extract of the herb complex is characterized by being in the form of an essential oil extracted by a steam distillation method.

The vitamin C, the extract of the herb complex, and adenosine are contained in an amount of 0.1 to 1 wt% of vitamin C, 5 to 7 wt% of the herbal complex, and 0.1 to 1 wt% of adenosine with respect to 100 wt% of the total composition.

The vitamin C, the extract of the herb complex, and adenosine are contained in an amount of 0.5% by weight of vitamin C, 6% by weight of the herb complex, and 0.5% by weight of adenosine, based on 100% by weight of the total composition.

Wherein the composition is a cosmetic composition or a soap composition.

According to the present invention, there is an advantage of providing a composition for external application for skin having excellent antioxidation, whitening and wrinkle reducing activity.

1 is a graph showing experimental results according to Examples 1 to 4;
2 is a graph showing the results of an experiment according to Comparative Example 1. Fig.
3 is a graph showing the experimental results according to Comparative Example 2. Fig.
4 is a graph showing the experimental results according to Comparative Example 3. Fig.

The present invention relates to a composition for external application for skin, which comprises vitamin C, an extract of a herb complex, and adenosine, thereby exhibiting antioxidation, improvement of skin wrinkles and skin whitening effect.

Hereinafter, the present invention will be described in detail.

Vitamin C used in the present invention is one of the most easily accessible vitamins that can be contained in almost all foods and acts as a strong reducing agent to activate collagen synthesis enzymes. This vitamin C has a powerful antioxidant function, it has collagen promotion, melanin production inhibition, anti-inflammatory effect, and it reduces wrinkles, makes skin elastic, and has excellent effect on whitening and inflammation relief. These vitamin C can be ingested through the food or absorbed directly into the skin. When ingested, the amount reaching the skin is minimal and it is excreted within about 3 hours after absorption. .

Herbs are rich in caffeic acid derivatives, flavonoids, and alkaloids, which are effective in blocking ultraviolet rays that promote skin aging. In addition, there are various useful substances that can react with generated free radicals to remove free radicals. The free radical scavenging ability of such a herb has the effect of inhibiting the initiation of the chain reaction by eliminating the initiating radical and inhibiting the radical initiation reaction by binding to the catalytic transition metal, , As well as to block the chain reaction so that continuous hydrogen deodorization by active radicals is not possible. Such antioxidant activity is used as a multifunctional raw material which simultaneously performs various actions such as blocking of the generation of radicals, elimination of the generated radicals and recovery of damaged cells through synergistic action with various useful physiological substances coexisting in the herb Bar, antioxidant, as well as effective for whitening and wrinkle improvement.

Such herbs are preferably a mixture of camomile, rosmary, lavender, tea tree, cypress, Sage and hibiscus. This is because mixed use of one of the above herbicides alone is more effective for free radical scavenging and recovery of damaged cells, and is superior to other kinds of herbicides.

First, the above-mentioned chamomile is an apricot flower having a skin soothing effect through skin moisturizing, sterilization and disinfection. The rosemary is excellent in antimicrobial and sterilizing action, has excellent moisturizing effect, and softens the skin. The lavender alleviates tension, protects the skin by preventing the propagation of germs, and has excellent antibacterial effect. The tea tree is effective against acne because of strong antifungal action and antibacterial action, and also has an anti-inflammatory effect. The cytoplasm is excellent in astringent action, excellent in sterilization and insecticidal effect, and suitable for aging skin. The sage is involved in alleviating skin irritation caused by external stimuli or the like, and loses or removes the activity of foreign substances harmful to the skin to help manage the problematic skin. It is also involved in tensing and elasticizing the skin. The hibiscus has the effect of gently removing keratin, cleanses skin tone gently, relaxes toxins accumulated in skin, and helps skin calm.

In the present invention, the composition ratio of the hub complex, that is, the mixing amount of each hub is not limited, but it is most preferable that all the seven kinds of hubs are mixed in the same amount, that is, the same weight ratio.

The mixture of chamomile, rosemary, lavender, tea tree, cypress, sage and hibiscus is immersed in extraction solvent for 1 to 15 hours at 4 to 40 ° C. The extract of the herb complex is used in the present invention. Or extracts obtained by heating at 40-100 ° C for 2 to 20 hours using an extraction solvent, followed by concentration under reduced pressure. The extraction solvent may be one or more selected from the group consisting of water containing purified water, C1 to C4 lower alcohols such as methanol and ethanol, and butylene glycol. The amount of the extraction solvent to be used is 1 to 20 It is appropriate to be a volume multiplier. In addition, the concentrated solution can be lyophilized if necessary after the concentration under reduced pressure. The method of concentration under reduced pressure and freeze-drying are already well known in the field to which this technique belongs, and thus a detailed description thereof will be omitted.

As the extract of the herb complex, it is possible to use an extract of the essential oil extracted by the steam distillation method. The steam distillation method means that water is injected into the essential oil extractor and the extract is activated to extract the oil. And the detailed description thereof will be omitted.

Adenosine, which is another component of the present invention, is a nucleoside involved in signal transduction outside the cell and is directly involved in cell growth, differentiation and homeostasis. The adenosine is generated in the energy metabolism stage and binds to the adenosine receptor connected to the outside of the cell and passes the bio signal transmission process through adenyl cyclase (AC) and phospholipase C (PLC) Finally expresses a gene involved in cell differentiation, anti-inflammation, wound healing, and functions to the skin. This adenosine has the effect of preventing aging and wrinkling of the skin, and is excellent in stability and persistence when penetrating the skin because it is an intracellular component. In addition, adenosine is a raw material for wrinkle improvement remedy in KFDA, which can be used at night or during the day, unlike retinol.

The composition containing the vitamin C, the herbal complex extract, and the adenosine as an active ingredient may be any one of a cosmetic composition or a soap composition used as an external preparation for skin, and may contain vitamin C 0.1 to 1 wt% based on the total weight of the cosmetic composition or soap composition Preferably 5 to 7% by weight of the extract of the herb complex and 0.1 to 1% by weight of the adenosine, more preferably 0.5% by weight of the vitamin C, 6% by weight of the herb complex and 0.5% . This is a blending ratio in which the antioxidative, whitening and wrinkle-reducing effects are most excellent by the interaction of the above-mentioned ingredients. In addition, the remaining components of the composition other than these components may be components of various known cosmetic compositions or soap compositions, and a detailed description thereof will be omitted.

The formulations of the cosmetic composition may be various known formulations such as skins, emulsions, creams, essences, etc., but are not limited thereto. In addition, it is natural that soap can also be manufactured into various formulations such as foam, liquid, and solid.

Hereinafter, the present invention will be described in detail with reference to the following Examples and Experimental Examples.

However, the following examples and experimental examples are illustrative of the present invention, and the content of the present invention is not limited by the following examples and experimental examples.

Example 1.

100 g of each of chamomile, rosemary, lavender, tea tree, cypress, sage and hibiscus was prepared as a dry weight, and the resulting mixture was added to 5 L of water and distilled by hot water for 5 days at 85 to 95 ° C to obtain an extract of the herb complex .

To 30 ml of the herbal complex extract, 2.5 g of vitamin C and 2.5 g of adenosine were mixed.

Example 2.

200 g of each of chamomile, rosemary, lavender, tea tree, cypress, sage and hibiscus was prepared as dry weight, and the resulting mixture was added to 14 L of water and heated at 40 to 50 ° C for 5 hours to obtain an extract. The mixture was filtered through a filter paper, and concentrated under reduced pressure at 60 DEG C to obtain a solid content of 30 g. Then, 2.5 g of vitamin C and 2.5 g of adenosine were mixed.

Example 3.

The procedure of Example 2 was repeated except that 80% ethanol was used as the extraction solvent.

Example 4.

Butylene glycol was used as an extraction solvent.

Comparative Example 1

The same procedure as in Example 1 was carried out except that vitamin C and adenosine were not used.

Comparative Example 2

The same procedure as in Example 1 was carried out except that adenosine was not used.

Comparative Example 3

The same procedure as in Example 1 was carried out except that vitamin C was not used.

Experimental Example 1. Free radical scavenging effect experiment

A stable 1,1-diphenyl-2-picrylhydrazyl radical was prepared by using the samples of the above-described Examples and Comparative Examples to improve Fujita's method. (MS Blois, Antioxidant determinations by the use of astable free radical, Nature, 29, pp1199-1200, 1958) A sample was added to 1 ml of a 0.2 mM diphenyl-2-picryl hydrazine (DPPH) And the reaction was allowed to proceed at room temperature for 10 minutes. The maximum absorbance at 525 nm was measured to determine the free radical scavenging effect (%). The free radical scavenging ability was calculated as shown in the following formula (1).

(1)

Free radical scavenging ability (%) = [1- (A-A ') / (B-B')]

A: Absorbance at 525 nm of the reaction solution in which the sample is spiked.

A ': Absorbance of the reaction solution without addition of DDPH at 525 nm.

B: Absorbance of the reaction solution without addition of the sample at 525 nm.

B ': Absorbance at 525 nm of the reaction solution without addition of the sample and DDPH.

Experimental Example 2: Tyrosinase inhibitory activity (Tyrosinase inhibitory activity)

The inhibitory activity of tyrosinase was measured. 1.0 ml of a 0.1 M potassium phosphate buffer (pH 6.8), 1.0 ml of a 0.3 mg / ml L-tyrosine aqueous solution and 0.1 ml of 1250 units / ml mushroom tyrosinase were mixed After that, 0.2 ml of each sample was added according to the concentration, and the enzyme reaction was carried out at 37 ° C for 10 minutes. The absorbance of the reaction solution was measured at 480 nm and the enzyme inhibition activity of the sample was measured according to the following equation (2).

(2)

(%) Of tyrosinase inhibition = [(A-B) / A] x 100

A; Absorbance of reaction solution without sample added at 475 nm.

B: Absorbance at 475 nm of the reaction solution to which the sample was added.

Experimental Example 3 Elastase Inhibitory Effect Experiment

The elastase activity inhibitory effect was measured. 0.267 M Tris solution was adjusted to pH 8.0 with 0.267 M hydrochloric acid (HCl) solution. Substrate solution 20 (20 ml) containing 8.8 mM Elastase substrate Succ-Ala-Ala-Ala-p-nitroanilide standard , Each sample was diluted to a predetermined concentration in purified water, and 100 각 of each sample was added thereto. 20 쨉 l of an enzyme solution having 10 / / ml of a standard product of Porcine Pancreatic Elastase was added and mixed After reaction at 25 ° C for 15 minutes, the amount of p-nitroaniline produced was measured for absorbance B at a wavelength of 410 nm. 20 占 퐇 of substrate solution, 20 占 퐇 of purified water and 100 占 퐇 of each sample prepared by diluting each sample in purified water to a predetermined concentration were added to 60 占 퐇 of the same buffer as above, Absorbance C was measured. The absorbance A was measured using a liquid obtained by operating the same method as that of the sample solution with 100 μl of purified water instead of the above sample as a blank test solution. 120 μl of purified water was added in place of the enzyme solution and the sample solution, Was determined as a colorimetric semen and the absorbance D thereof was measured to obtain an elastase inhibition rate (%) as shown in the following equation (3).

(3)

Elastase inhibition rate (%) = [(BC) / (AD)] 100

The results of each experimental example are shown.

First, the free radical scavenging rate (%), the tyrosinase inhibiting rate (%), and the elastase inhibiting rate (%) according to Examples were as shown in Table 1 and FIG. The results were obtained from herbal complex extracts at 6%, vitamin C at 0.5% and adenosine at 0.5%.

(%), Tyrosinase inhibition rate (%) and elastase inhibition rate (%) according to Examples 1 to 4, division
Inhibition (%) Free radical Tyrosinase Activity Elastase Activity Example 1 97.258 92.128 86.017 Example 2 96.325 91.253 85.251 Example 3 97.125 90.251 83.214 Example 4 97.521 91.572 852.158

The free radical scavenging rate (%), tyrosinase inhibiting rate (%) and elastase inhibiting rate (%) of Comparative Examples 1 to 3 are shown in Tables 2 to 3 and Figs.

Free radical scavenging rate (%), tyrosinase inhibiting rate (%) and elastase inhibiting rate (%) according to the content of Comparative Example 1 division Inhibition (%) Free radical Tyrosinase Activity Elastase Activity 0.1 (%) 17.26619 4.230769 16.5109 0.2 (%) 30.21583 13.07692 25.8567 0.4 (%) 45.61151 21.92308 31.15265 0.8 (%) 71.65468 34.61538 33.33333 1.6 (%) 87.19424 42.69231 34.26791 3.2 (%) 90.50361 47.30769 42.67913 6.4 (%) 93.81295 53.84615 46.41745

Free radical scavenging rate (%), tyrosinase inhibiting rate (%) and elastase inhibiting rate (%) according to Comparative Examples 2 and 3, division
Inhibition (%) Free radical Tyrosinase Activity Elastase Activity Comparative Example 2 96.565 80.617 60.031 Comparative Example 3 92.145 82.056 65.076

As can be seen from Tables 1 to 3 and Figs. 1 to 3, the herbal complex extract of the present invention, the composition containing vitamin C and adenosine exhibits a free radical scavenging ratio (%), a tyrosinase inhibiting rate (% And the inhibition rate (%) were all excellent, and it was found that the antioxidative, whitening and wrinkle reducing effects were excellent.

In contrast, in Comparative Example 1 using only the herbal complex extract, the free radical scavenging ratio (%), the tyrosinase inhibiting rate (%) and the elastase inhibiting rate (%) were both higher, but the tyrosinase inhibitory rate The inhibition rate of starch was only about 50%, and it was confirmed that the effect of whitening and wrinkle reduction was weak.

In addition, in Comparative Example 2 containing 6% herbal complex extract and 0.5% vitamin C, it was confirmed that the inhibition rate of elastase was not good and the inhibition rate of tyrosinase was also below 90%. In the case of Comparative Example 3 containing 6% herbal complex extract and 0.5% adenosine, the inhibition rate of elastase was also poor and the inhibition rate of tyrosinase was also below 90%.

Accordingly, it was confirmed that the herbal complex extract, vitamin C, and adenosine together can provide excellent antioxidative, whitening and wrinkle-reducing effects as in Examples 1 to 4 of the present invention.

Formulation Example 1. Preparation of cream.

The raw materials as shown in Table 4 below were prepared by using the ingredients in the oil as the oil components and the components soluble in the purified water as the water components at 80 to 85 ° C The emulsion is neutralized with a carbomer and then cooled, inverted and defoamed to complete the preparation.

The content (weight%) of Formulation Example 1 Raw material content The composition of Example 1 7.0 glycerin 6.00 Squalane 6.00 Isohexadecane 5.50 Cetyl octanoate 4.00 Glyceryl stearate 2.50 Polysolite 60 1.00 FAGE -100 stearate 1.00 Stearic acid 1.00 lanolin 1.00 Laureates-12 1.00 Cetyl alcohol 0.50 Sorbitan sesquioleate 0.30 Carbomer 0.30 Triethanolamine 0.30 Methylparaben 0.20 Imidazolidinyl urea 0.15 Propylparaben 0.10 Combination fragrance 0.10 Purified water to 100

Formulation Example 2. Preparation of Lotion

The ingredients having the same contents as shown in Table 5 below were used as oil components in the oil as the oil component and the components soluble in the purified water as the water component in the general emulsification method according to the production method of the cream, The emulsion is neutralized with a carbomer and then cooled, inverted and defoamed to complete the preparation.

The content (weight%) of Formulation Example 2 division content The composition of Example 1 7 Squalane 3.50 Cetyl alcohol 3.00 glycerin 3.00 Glyceryl stearate 2.50 Cetyl octanoate 6.00 Sorbitan sesquioleate 1.50 FAGE -100 stearate 1.00 Sheer butter 0.50 Carbomer 0.30 Triethanolamine 0.30 Methylparaben 0.20 Imidazolidinyl urea 0.15 Propylparaben 0.10 Combination fragrance 0.10 Purified water to 100

Preparation Example 3. Preparation of Soap

0.4% by weight of alkoxyglycerin, 0.5% by weight of fragrance, 0.5% by weight of a moisturizer and 7% by weight of the composition of Example 1 were dissolved in 91.6% by weight of a refined soap base with stirring at 60 DEG C, So that a soap was produced.

In the above description, only the cosmetic and the solid soap of the formulations of cream and lotion are exemplified. However, it is possible to manufacture various formulations such as skin, gel and essence type according to the manufacturing method of usual cosmetics, It is natural that it is also possible to produce soap.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims

0.1 to 1% by weight of vitamin C, 5 to 7% by weight of an extract of herbal complex, and 0.1 to 1% by weight of adenosine with respect to 100% by weight of the total composition,
The herb complex is a mixture of chamomile, rosemary, lavender, tea tree, cypress, sage and hibiscus in equal amounts,
A composition having antioxidant, whitening and wrinkle-reducing effects, which is a cosmetic composition or a soap composition.

The method according to claim 1,
The extract of the herb complex may be prepared by immersing a mixture of chamomile, rosemary, lavender, tea tree, cypress, sage and hibiscus in an extraction solvent at 4 to 40 캜 for 1 to 15 hours or extracting at 40 to 100 캜 Wherein the composition is heated and extracted for 2-20 hours, and then concentrated.

The method according to claim 1,
Wherein the extract of the herb complex is in the form of an essential oil extracted by a steam distillation method.

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The method according to claim 1,
Wherein the vitamin C, the extract of the herb complex, and adenosine contain 0.5% by weight of vitamin C, 6% by weight of the herb complex, and 0.5% by weight of adenosine, based on 100% by weight of the total composition. / RTI >

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