KR102101949B1 - A skin-care agent or cosmetics containig Antrodia camphorata fruit body extract or Antrodia camphorata mycelium extract - Google Patents

Abstract

The present invention relates to a composition for external application for skin or a cosmetic composition containing a culture medium obtained from cultivation of a mycelium mushroom fruit extract or a culture of a mycelium mushroom mycelium, specifically, a culture solution obtained from the culture of a mycelium mushroom fruit extract or a culture of mycelium mushroom mycelia 0.001 It relates to a functional skin external preparation or cosmetic composition excellent in whitening effect, atopy improvement effect and skin irritation effect, characterized by containing ~ 90.0% by weight.

Description

A skin-care agent or cosmetics containig Antrodia camphorata fruit body extract or Antrodia camphorata mycelium extract

The present invention relates to an external preparation for skin or a cosmetic composition containing a jangji mushroom fruit extract or a jangjang mushroom mycelia extract, specifically, a filtrate obtained from the jangjang mushroom fruit extract or a culture obtained from the culture of jangjang mushroom mycelium It relates to a composition for external application for skin or a cosmetic composition.

delete

delete

Melanin is converted from tyrosine to dopa, dopaquinone by the action of tyrosinase present in pigment cells, and is produced through dopachrome. Melanin is present in the skin and protects the body from ultraviolet rays, etc., and exhibits important functions in regulating hormone secretion in the body. However, it is known that when melanin is overproduced, it forms blemishes, freckles, etc., promotes skin aging, and plays an important role in inducing skin cancer. Research and development to prevent overproduction of melanin has been actively conducted. Ascorbic acid (Japanese Patent Publication 4-9320), hydroquinone (Japanese Patent Publication 6-192062), kojic acid (Japanese Patent Publication 56-7710), arbutin (Japanese Patent Publication 4-93150), and plant extracts already inhibit tyrosinase. It has been used as a whitening cosmetic because it exhibits activity, but its stability in cosmetic formulations is poor, it is decomposed and colored, odor occurs, its efficacy and effect at a living body level are unclear, or its use is limited due to safety problems. to be.

On the other hand, atopic dermatitis (atopic dermatitis) is a typical disease that appears in people with atopic allergies, the cause of atopic dermatitis is still unknown, but 70% of atopic dermatitis patients had a history of atopic dermatitis, in people with allergic constitution It is understood as one of diseases caused by genetic factors or immune diseases because it is accompanied by other allergic diseases such as allergic rhinitis and asthma. In the treatment of atopic dermatitis, drugs such as adrenal cortical hormone, which can expect a high pharmacological effect, are used, but side effects of the adrenal cortical hormone are problematic, and rebound by stopping the use of the adrenal cortical hormone (stop the use of the drug) It is also well known that the later stages of dramatic lesions occur. As a countermeasure for side effects, nonsteroidal anti-inflammatory drugs or antihistamines, which do not contain hormones such as adrenal cortical hormone, are used, but development of an atopic dermatitis-free improvement or treatment without side effects is urgently needed.

Recently, in order to reduce skin irritation caused by various chemicals, a lot of attention has been focused on functional cosmetics having functions such as antioxidant, anti-wrinkle, whitening, and itching relief obtained from natural extracts. For example, U.S. Patent Publication No. 5,972,341 relates to the wrinkle improvement effect of Commiphora mukul extract, Japanese Patent Publication No. Hei 9-672662 relates to a seaweed extract having a hyaluronidase inhibitory effect, Japan Patent Publication No. Hei 10-85905 relates to the effect of improving the skin texture of Fucoidan extracted from Wakame, and Japanese Patent Publication No. Hei 2-245087 relates to the antioxidant effect of the extract of genus Sargassum, Korea Patent No. 0431076 is a cosmetic composition for improving the healing of atopic skin, including extracts of platinum, shiso, echinacea, and fermented soybeans, and Korea Patent No. 0511494 is an extract and composition for the treatment of atopic dermatitis, including sewage, siyeop, and illite About.

In order to solve this problem of skin, a lot of cosmetics have been developed using natural or medicinal and edible mushroom extracts to reduce skin irritation due to various chemicals. As a cosmetic composition using extracts of known mushrooms, snow flower cordyceps sinensis (Korean Patent No. 0340185), Phellinus linteus, Skirt mushroom (Korea Patent No. 0681703, No. 0295623), Leafy Mushroom (Korea Patent No. 0438009), Shiitake mushroom Etc., and research on the anti-aging effect of these extracts has been continued.

delete

Mushrooms used for medicinal and edible purposes have numerous examples and types, but the mushrooms used and studied as cosmetic compositions are still known as a part.
On the other hand, Ujangji Mushroom ( Antrodia camphorata ) is a rare mushroom called Ujangji or Jangji that is parasitic on camphor trees that are more than 100 years old, which survive in the high altitude of 1,000 m above sea level in Taiwan. In China, it is traditionally known to be effective against hypertension, liver cancer, inflammation, itching, abdominal pain and diarrhea.

delete

 It has not been determined whether these jangjangji mushrooms have a whitening effect, a skin irritation relief effect, and an atopy improvement effect as a skin external preparation.

The Republic of Korea Patent No. 10-0438009 (a cosmetic composition containing an extract of the mycelium of the leaf mushroom) from the mycelium of the leaf mushroom or the culture medium of the leaf mushroom which has an antioxidant effect, a skin cell proliferation and collagen production promoting effect and skin melanin production inhibitory effect It has been described for a cosmetic composition containing the obtained extract, but it has not been described about the use of the Ujiji mushroom fruiting body extract or the Ujangji mushroom mycelium culture medium. Korean Registered Patent No. 10-0340185 (a cosmetic composition for anti-wrinkle containing an extract of Snow Cordyceps sinensis) has been described for an excellent cosmetic composition for anti-wrinkle by containing an extract of an eye-flower Cordyceps sinensis, but this includes: No point has been described for using a mycelium culture.

An object of the present invention is to provide a skin external preparation or cosmetic composition having a whitening, atopy improvement and skin irritation effect using a natural extract.

In addition, the present invention to solve the above problems, to provide a composition for external application for skin or cosmetic composition, characterized in that it contains a culture medium obtained from a culture of a higher fungi jangjang mushroom fruiting body extract or a jangjang mushroom mycelium as an active ingredient. There is this.

In addition, an object of the present invention is to provide a method for efficiently obtaining a fruiting body extract or a mycelium extract of Ujangji mushroom having a complex effect of whitening, atopy improvement and skin irritation.

In order to achieve the above object, the present invention provides a composition for external application for skin or a cosmetic composition, characterized in that it contains a culture medium obtained from cultivation of a mycelium mushroom fruiting body extract or a mycelium mushroom mycelia as an active ingredient.

Hereinafter will be described in detail with respect to the problem solving means of the present invention.

The external preparation or cosmetic composition of the present invention is obtained from the culture of jangjang mushroom fruit extract or jangjang mushroom mycelium to provide a skin external preparation or cosmetic composition excellent in whitening effect, atopic dermatitis improvement / prevention or treatment effect, and skin irritation relaxation effect. It is characterized by containing the obtained culture medium as an active ingredient.

The fruiting body extract of jangjangji mushroom of the present invention can be obtained by extracting the fruiting body of jangjangji mushroom, and the type of fruiting body of jangjangji mushroom is not limited to a specific type, and both natural and artificial cultures can be used.

In addition, the method for extracting the fruiting body extract of Ujangi mushroom of the present invention can use a method commonly used in the technical field to which the present invention pertains. For example, about 2 to 20 times, preferably about 2 to 5 times the lower (C1-C4) alcohol, such as methanol or ethanol, to the fruit body powder obtained by freeze-drying and pulverizing the fruit body of jangjangji mushroom As a polar solvent or a mixed solvent thereof, extraction methods such as cold immersion extraction, reflux cooling extraction, hot water extraction, ultrasonic extraction, and ultra-high pressure extraction at an extraction temperature of 10 ° C to 30 ° C may be used.

Further, preferably, the extract thus obtained may be filtered, concentrated under reduced pressure, or freeze-dried to be used in a polar solvent. For example, the obtained extract is filtered, transferred to a concentration tank, concentrated under reduced pressure and freeze-dried at 50 ° C. or less, and purified water, ethanol, butylene glycol so that the obtained reduced pressure concentrate and freeze-dried product contains 0.001 to 70.0% by weight, The extract may be prepared using one or more solvents selected from propylene glycol.

In addition, the fruiting body extract of jangjangji mushroom of the present invention may mean the polysaccharide itself contained as an active ingredient in the extract thus obtained.

On the other hand, the mycelial mushroom mycelium extract of the present invention is obtained by extracting the mycelial mushroom mycelium, and the extraction method may use a method commonly used in the technical field to which the present invention pertains. For example, it may be a hot water extract of the mycelium powder obtained by freeze-drying and pulverizing the mycelium mushroom, or an organic solvent extract extracted using methanol, ethanol, butanol or a mixed solvent thereof.

On the other hand, the culture solution obtained from the culture of the mycelium jangjang mushroom can be used as it is, or dried (or lyophilized) can be used in powder form. Preferably, the culture medium can also be used by filtration. Specifically, after culturing the mycelium mushrooms in liquid, the mycelium is removed through centrifugation, and the filtrate obtained by filtering the culture filtrate with a paper filter can be used. At this time, culture is meant to include 'fermentation'.

In addition, the culture medium obtained from the culture of the mycelium mushroom mycelium extract or the mycelium mushroom mycelia of the present invention may be a polysaccharide extracted from the hot water extract, the organic solvent extract or the filtrate.

In addition, the culture medium obtained from the culture of the mycelium mushroom mycelium extract or the mycelium mushroom mycelium of the present invention is preferably an extract of the mycelium mushroom mycelium using one or more animals or plants or herbal medicines as a substrate. Here, the animal or plant or medicinal herb is not particularly limited, and the animal or plant or medicinal herb commonly used in the art to which the present invention pertains may be used as a substrate.

The culture medium obtained from the cultivation of the mycelium mushroom fruiting body extract of the present invention or the mycelium mushroom mycelium extract or the mycelium mushroom mycelia of the present invention thus obtained has excellent whitening effect, anti-inflammatory effect on atopic dermatitis, and skin irritation relaxation effect, so it is multifunctional. It can be used in external skin preparations or cosmetic compositions.

Meanwhile, the skin external preparation or cosmetic composition of the present invention preferably contains 0.001 to 90.0% by weight of the culture solution obtained from the culture of the jangjang mushroom fruit extract or the jangjang mushroom mycelium extract or the jangjang mushroom mycelium with respect to the entire composition, 0.001% by weight If included below, the skin improvement effect is insignificant, and when it exceeds 90.0% by weight, it is because it is not economical because it is less efficient compared to the amount of material input.

On the other hand, in the present invention, the meaning of "included as an active ingredient" refers to the extent to which the skin external agent or cosmetic composition of the present invention can exhibit the effect of prevention and suppression of skin diseases, the fruit extract of jangjangji mushroom in the external composition or cosmetic composition It means that the mycelium mushroom mycelium extract or the culture solution obtained from the culture of the mycelium mushroom mycelium is added, and that it is formulated into various forms by adding various components as subcomponents for drug delivery and stabilization. .

On the other hand, the composition for external application for skin of the present invention is preferably a pharmaceutical composition, for example, as a pharmaceutical composition, a warning agent (PLASTERS), a lotion agent (LOTIONS), a linement agent (LINIMENTS), a liquid agent (LIQUIDS AND SOLUTIONS) , Aerosols (AEROSOLS), EXJET (EXTRACTS), Ointment (OINTMENTS), Fluid EX (FLUIDEXTRACTS), Emulsion (EMULSIONS), Suspension (SUSPESIONS), Capsules (CAPSULES), Cream (CREAMS), Soft or Hard There are gelatin capsules, patch and sustained release agents.

delete

delete

On the other hand, the pharmaceutical composition of the present invention is a pharmacologically acceptable base; Carriers; Excipients; Binders comprising starch, tragacanth rubber, gelatin, molasses, polyvinyl alcohol, polyvinyl ether, polyvinyl pyrrolidone, hydroxypropyl cellulose, methyl cellulose, ethyl cellulose and carboxymethyl cellulose; Crushing agents including agar, starch, gelatin powder, sodium carboxymethyl cellulose, calcium carboxymethyl cellulose, crystalline cellulose, calcium carbonate, sodium hydrogen carbonate and sodium alginate; Lubricants including magnesium stearate, talc and hydrogenated vegetable oil; And colorants. Examples of the carrier and excipients include lactose, glucose, sucrose, mannitol, potato starch, cornstarch, calcium carbonate, calcium phosphate, and cellulose. In addition to the above, additives such as stabilizers, dissolution aids, adjuvant fragrances such as transdermal absorption accelerators, and preservatives may be further added.

On the other hand, for many patients, the standard dosage for the composition for external application for skin according to the present invention can be changed by individual characteristics of the patient, and a practically skilled clinician is an ideal dosage for the composition for external application for skin for the patient The most appropriate treatment strategy can be established in terms of dosage and dosing regimen, eg specific needs and patient overall conditions. For the composition for external application for skin of the present invention, a number of references can be referred to in determining an appropriate dosage.

In addition, suitable dosages of the composition for external application for skin of the present invention may be generally determined in vitro or in an animal model. For example, various concentrations of the composition for external application for the skin of the present invention may be added to a target cell in vitro to determine an appropriate dosage.

On the other hand, the pharmaceutical composition is preferably for atopy prevention or treatment.
On the other hand, the cosmetic composition of the present invention is preferably a cosmetic composition for skin whitening, atopy skin improvement and skin irritation relief, for example, as a formulation of the cosmetic composition, lotion, gel, water-soluble liquid, cream, essence, oil in oil Basic cosmetic formulations consisting of (O / W) type and water-in-oil (W / O) type; A color cosmetics formulation consisting of an oil-in-water or water-in-oil makeup base, foundation, skin cover, lipstick, lip gloss, face powder, two-way cake, eye shadow, cheek color, and eyebrow pencils; There is this.

According to the present invention, it is possible to obtain a good yield of Uji-ji mushroom fruiting body extract or Uji-ji mushroom mycelia culture, which exhibits a complex effect of whitening, atopy improvement and skin irritation alleviation, and using this to produce a multifunctional skin external preparation or cosmetic composition can do.

Hereinafter, the contents of the present invention will be described in more detail in the following Examples. However, the scope of rights of the present invention is not limited to the following examples, but includes modifications of technical ideas equivalent thereto.

Example  1: Preparation of fruit extract of Ujangji mushroom

Beef Mushroom ( Antrodia camphorata ) The fruiting body powder obtained by freeze-drying and pulverizing the fruiting body was refluxed 3 times for 5 hours in an aqueous solution containing 70% (v / v) ethanol, cooled, and then filtered with Whatman # 5 filter paper. . The filtered extract was concentrated under reduced pressure and freeze-dried at 50 ° C. or less, and then dissolved in a mixed solvent of purified water, ethanol, butylene glycol, and propylene glycol to contain 15% by weight of the obtained freeze-dried product (hereinafter, referred to as' Ujangji mushrooms). Fruiting body extract).

Example  2: Preparation of mycelium extract of Ujangji mushroom

Ujangji mushroom used in the experiment ( Antrodia camphorata ) strain was isolated directly from the fresh fruiting body, and the yeast-wort agar medium (yeast extract 3 g, wort 3 g, glucose 10 g, peptone 5 g, agar 20 g, distilled water 1 liter) It was incubated in all test tubes, stored at 4 ° C, and subcultured every month for use. After aseptically homogenizing the mycelium grown on the sloped medium, and making it a simple complex medium containing 3% glucose and 1.0% yeast extract, and inoculating the liquid medium adjusted to pH 5.5 to 5% (v / v), Incubation was carried out for 10 days in the fermenter under conditions of a temperature of 27 ° C, a rotation speed of 150 rpm, and an aeration amount of 1.5 vvm.

After the completion of the culture, the mycelium was recovered through centrifugation, and then extracted by the method described in Example 1 to prepare a mycelium extract (hereinafter referred to as a 'mycelia mushroom mycelium extract').

Example  3: Preparation of culture solution obtained from cultivation of mycelium mushrooms

Ujangji mushroom used in the experiment ( Antrodia camphorata ) strain was isolated directly from the fresh fruiting body, and the yeast-wort agar medium (yeast extract 3 g, wort 3 g, glucose 10 g, peptone 5 g, agar 20 g, distilled water 1 liter) It was incubated in all test tubes, stored at 4 ° C, and subcultured every month for use. After aseptically homogenizing the mycelium grown on the sloped medium, and making it a simple complex medium containing 3% glucose and 1.0% yeast extract, and inoculating the liquid medium adjusted to pH 5.5 to 5% (v / v), Incubation was carried out for 10 days in the fermenter under conditions of a temperature of 27 ° C, a rotation speed of 150 rpm, and an aeration amount of 1.5 vvm.

After the completion of the culture, the culture filtrate from which the mycelium was removed through centrifugation was filtered with a paper filter (watman no.4) to obtain a filtrate (hereinafter referred to as a 'mycelia mushroom mycelium culture').

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

Experimental Example 1: Measurement of tyrosinase activity inhibitory effect

In this Experimental Example 1, arbutin, a well-known whitening agent, was used as a comparison sample to confirm the inhibitory effect of tyrosinase activity on the mycelial fruit extract obtained in Example 1 and the culture of mycelial mushroom fungus obtained in Example 3 above. .

Tyrosinase is an enzyme that promotes the oxidation process of a substance called tyrosine in vivo to produce melanin.

Tyrosinase was isolated and purified from mushrooms and was purchased and used by Sigma. The substrate L-Tyrosine is dissolved in 0.05 M sodium phosphate buffer solution (pH 6.8) to make a 0.1 mg / ml solution, and the fruit mixture of Uji mushrooms and the mycelium culture of Ujangji mushrooms is 0.05 M sodium phosphate buffer solution (pH 6.8), respectively. It was used after dissolving by adjusting to an appropriate concentration. 0.5 ml of the L-tyrosine solution was placed in a test tube, and 0.5 ml of the sample of the mycelium mushroom fruiting body extract and the culture of the mycelium mushroom mycelium were added, respectively, and allowed to stand for 10 minutes at 37 ° C incubator. Then, 0.5 ml of 200 unit / ml tyrosinase was added and reacted at 37 ° C for 10 minutes. After placing the test tube containing the reaction solution on ice and quenching to stop the reaction, the spectrophotometer was measured for absorbance at a wavelength of 475 nm. As a control group, 0.5 ml of buffer solution was used instead of the fruit extract of Ujangji mushroom and the mycelium culture of Ujangji mushroom.

The inhibition rate of tyrosinase activity of each sample was calculated according to Equation 1.

[Equation 1]

Inhibition rate of tyrosinase activity (%) = 100-[(Comparative group absorbance / control absorbance)] × 100

Sample name Tyrosinase inhibition rate (%) Control 0 Ujangji mushroom mycelium culture medium 0.02% by weight 51 Ujangji mushroom mycelium culture medium 0.01% by weight 41 Ujangji mushroom mycelium culture medium 0.005% by weight 35 Arbutin 0.2% by weight 54

delete

As a result of measuring the activity inhibitory effect of tyrosinase (Table 1), the mycelium culture of jangjang mushroom showed a tyrosinase activity inhibition rate similar to that of arbutin.

From the above results, it was possible to confirm the excellent tyrosinase activity inhibitory effect of the mycelium mushroom mycelium culture, and based on this, the skin whitening agent or cosmetic composition of the present invention was found to have excellent whitening effect.

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

Example  4 and Comparative example  1: Containing Ujangji mushroom fruiting body extract Cosmetic  Produce

In this Example 4, a cosmetic composition containing the fruiting body extract of Uji mushroom obtained in Example 1 was prepared.

The prepared cosmetic is in the form of a cream, and the composition is shown in Table 2 below.

First, the phase B) recorded in Table 2 was heated and stored at 70 ° C, and phase B) was added to phase A), followed by pre-emulsification, uniformly emulsified with a homomixer, and gradually cooled to give a cream (Example 3, Comparative Example) 1) was prepared.

Raw material Example 4 (% by weight) Comparative Example 1 (% by weight) end Stearyl alcohol 8 8 Stearic acid 2 2 Stearic acid cholesterol 2 2 Squalane 4 4 2-octyldodecyl alcohol 6 6 Polyoxyethylene (25 mol addition) alcohol ester 3 3 Glyceryl monostearic acid aster 2 2 I Ujangji mushroom fruiting body extract One - Propylene glycol 5 5 Purified water Until 100 Until 100

Example  5 and Comparative example  2: Ujangji mushroom mycelium culture medium containing Cosmetic  Produce

In Example 5, a cosmetic composition containing the mycelium culture of jangji mushroom obtained in Example 3 was prepared.

The prepared cosmetic is in the form of a cream, and the composition is shown in Table 3 below.

First, the phase B) recorded in Table 3 was heated and stored at 70 ° C, and phase B) was added to phase A), followed by pre-emulsification, uniformly emulsified with a homomixer, and gradually cooled to cream (Example 4, Comparative Example). 2) was prepared.

Raw material Example 5 (% by weight) Comparative Example 2 (% by weight) end Stearyl alcohol 8 8 Stearic acid 2 2 Stearic acid cholesterol 2 2 Squalane 4 4 2-octyldodecyl alcohol 6 6 Polyoxyethylene (25 mol addition) alcohol ester 3 3 Glyceryl monostearic acid aster 2 2 I Ujangji mushroom mycelium culture One - Propylene glycol 5 5 Purified water Until 100 Until 100

Experimental Example 2: Measurement of improvement effect of cosmetic products on atopic dermatitis

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

In this Experimental Example 2, clinical trials were conducted as follows to compare and measure the improvement effect of the cosmetic preparations prepared in Examples 4 to 5 and Comparative Examples 1 to 2 on atopic dermatitis.

The effect of improving atopic dermatitis was investigated in 30 patients with atopic dermatitis who have been suffering from atopic dermatitis for more than 2 years as atopic patients aged 5 to 30 years. To the same person, Example 4 or Example 5 was applied to the left hand, and Comparative Example 1 or Comparative Example 2 was cleaned to the right hand every evening, and then applied to the skin once a day for 60 days to measure the degree of atopic dermatitis improvement. Measurement was conducted by sensory evaluation by survey. The results are shown in Tables 4 and 5 below.

very good good so so Example 4 80% (24 people) 20% (6 people) 0% Comparative Example 1 10% (3 persons) 20% (6 people) 70% (21 people)

very good good so so Example 5 70% (21 people) 30% (9 people) 0% Comparative Example 2 10% (3 persons) 20% (6 people) 70% (21 people)

As a result of measuring the improvement effect on cosmetic atopic dermatitis (Table 4, Table 5), Example 3 containing the fruit extract of jangjangji mushroom and Example 4 containing the culture of mycelium jangjang mushroom than Comparative Examples 1 to 2 It was confirmed that the atopic skin improvement effect is excellent.

From the above results, it was confirmed that the skin external preparation or cosmetic composition of the present invention has an excellent atopic dermatitis improvement effect.
Experimental Example 3: Measurement of skin irritation effect by SLS
In this experimental example, the effect of alleviating the irritation of the cosmetic preparations prepared in Examples 4 to 5 was evaluated by a human patch test.
After mixing 1% of sodium lauryl sulfate (SLS) that causes irritation to the general cosmetic prescription (cream, lotion, skin, essence) and the cosmetics of Example 4 or 5, and applying for 24 hours, 48 hours, 72 hours, The effect of stimulation relaxation was evaluated based on the stimulus-inducing index.
50 healthy men and women between 20 and 50 years of age were patched with 0.3 mg of FINN CHAMBER (FINLAND) on the upper arm of the arm, and the acute irritation index was evaluated after 24 hours. After evaluation, the same amount of product was applied to the same site again, and the delayed irritation index after 48 hours and 72 hours was evaluated.
As a result of measuring the effect of alleviating skin irritation caused by SLS, the part that was applied with SLS alone showed a red irritation on the skin after 24 hours, but the Example 4 and the culture of mycelium mushroom mycelium culture containing the fruit extract of U. The containing Example 5 did not cause any skin attacks even after 24 hours, 48 hours, and 72 hours after application.
From the above results, it was confirmed that the skin irritation caused by the base material (surfactant, fragrance, alcohol) that causes irritation when mixed with the cosmetic of Ujangji mushroom fruit body extract and Ujangji mushroom mycelium can be reduced.

delete

delete

delete

delete

delete

delete

Example  6: Preparation of lotion containing Ujangji mushroom fruit body extract

Polypyrrolidone 0.05 g, oleyl alcohol 0.1 g, polyoxyethylene monooleate 0.2 g, fragrance 0.2 g, paraoxybenzoic acid methyl ester 0.1 g, a small amount of antioxidant, and a small amount of pigment are mixed and dissolved in 8 g of 95% ethanol. Did. The mixed liquid was added to and stirred by dissolving 0.05 g of jangjang mushroom fruit body extract obtained in Example 1 and 5 g of glycerin in 85.33 g of purified water, followed by stirring to prepare a lotion containing jangjang mushroom fruit body extract.

Example  7: Preparation of lotion containing Ujangji mushroom mycelium culture

Polypyrrolidone 0.05 g, oleyl alcohol 0.1 g, polyoxyethylene monooleate 0.2 g, fragrance 0.2 g, paraoxybenzoic acid methyl ester 0.1 g, a small amount of antioxidant, and a small amount of pigment are mixed and dissolved in 8 g of 95% ethanol. Did. 0.05 g of the mycelium mushroom mycelium culture obtained in Example 3 and 5 g of glycerin were dissolved in 85.33 g of purified water, and the mixture was added and stirred to prepare a cosmetic lotion containing the mycelium mushroom mycelium culture.

Example  8: Preparation of milkweed containing fruit extract of Ujangji mushroom

1.2 g of cetyl alcohol, 10 g of squalane, 2 g of vaseline, 0.2 g of paraoxybenzoic acid ethyl ester, 1 g of glycerin monoesteraide, 1 g of polyoxyethylene (20 mol parts) monooleate and 0.1 g of fragrance at 70 ° C The mixture was heated and dissolved, and 0.5 g of the oyster mushroom fruit body extract obtained in Example 1, 5 g of dipropylene glycol, 2 g of polyethylene glycol-1500, 0.2 g of triethanolamine, and 76.2 g of purified water were dissolved by heating to 75 ° C. The mixture was emulsified by mixing and cooled to prepare an oil-in-water (O / W) emulsion.

Example  9: Preparation of latex containing mycelium mycelium culture

1.2 g of cetyl alcohol, 10 g of squalane, 2 g of vaseline, 0.2 g of paraoxybenzoic acid ethyl ester, 1 g of glycerin monoesteraide, 1 g of polyoxyethylene (20 mol parts) monooleate and 0.1 g of fragrance at 70 ° C The mixture was heated and dissolved, and 0.5 g of jangjang mushroom mycelium culture medium obtained in Example 3, 5 g of dipropylene glycol, 2 g of polyethylene glycol-1500, 0.2 g of triethanolamine, and 76.2 g of purified water were dissolved by heating to 75 ° C. The mixture was emulsified by mixing and cooled to prepare an oil-in-water (O / W) emulsion.

Example  10: containing jangji mushroom fruiting body extract Essence  Produce

Polyoxyethylene sorbitan monooleate 1.2 g, chitulose 0.3 g, sodium hyaluronate 0.2 g, vitamin E-acetate 0.2 g, sodium licorice 0.2 g, paraoxybenzoic acid ethyl ester 0.1 g in 5 g of 95% ethyl alcohol , 1 g of jangji mushroom fruit body extract obtained in Example 1 and an appropriate amount of pigment were mixed to prepare a cosmetic liquid.

Example  11: containing the culture of mycelium mushrooms Essence  Produce

Polyoxyethylene sorbitan monooleate 1.2 g, chitulose 0.3 g, sodium hyaluronate 0.2 g, vitamin E-acetate 0.2 g, sodium licorice 0.2 g, paraoxybenzoic acid ethyl ester 0.1 g in 5 g of 95% ethyl alcohol , 1 g of the mycelium mushroom mycelium culture obtained in Example 3 and an appropriate amount of pigment were mixed to prepare a cosmetic liquid.

Claims (18)

delete delete delete delete delete A cosmetic composition for skin whitening, characterized in that it contains any one of a culture medium obtained from the culture of Ujangji mushroom mycelium extract, Ujangji mushroom mycelium extract or Ujangji mushroom mycelium as an active ingredient.
delete delete delete delete delete delete delete delete delete delete delete delete