KR102067283B1 - A skin-care agent or cosmetics containig Morchella esculenta fruit body extract or Morchella esculenta mycelium extract - Google Patents

Abstract

The present invention relates to a skin external preparation or cosmetic composition containing a culture solution obtained from the culture of morel fruit body extract or morel mycelium mycelium, specifically 0.001 ~ 90.0% by weight of the culture solution obtained from the culture of morel fruit body extract or morel mushroom mycelium It relates to a functional skin external preparation or cosmetic composition excellent in whitening, atopy improvement and skin irritation effect comprising a.

Description

A skin-care agent or cosmetics containig Morchella esculenta fruit body extract or Morchella esculenta mycelium extract}

The present invention relates to a skin external preparation or cosmetic composition containing the morel fruit body extract or morel mycelium mycelium extract, specifically, the skin external agent containing a culture solution obtained from the culture of the filtrate obtained from the morel fruit body extract or morel mushroom mycelium Or it relates to a cosmetic composition.

Melanin is converted from tyrosine to dopa, dopaquinone by the action of tyrosinase present in pigment cells, and is generated via dopachrome. Melanin is present in the skin to protect the body from ultraviolet rays and other important functions in the body's hormone secretion control. However, if melanin is overproduced, it is known to play a key role in forming blemishes, freckles, etc., promoting skin aging, and causing skin cancer, and research and development for preventing melanin overproduction are being actively conducted. Ascorbic acid (JP-A 4-9320), hydroquinone (JP-A 6-192062), kojic acid (JP-A-56-7710), arbutin (JP-A 4-93150), and plant extracts have already inhibited tyrosinase. It is used as a whitening cosmetic due to its activity, but its stability in cosmetic formulations is poor, and it is decomposed and colored, odors occur, its efficacy and effects on the biological level are unclear, or its safety is a problem and its use is restricted. to be.

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On the other hand, atopic dermatitis is a typical disease in people with atopic dermatitis. Although the cause of atopic dermatitis has not been clarified yet, 70% of patients with atopic dermatitis have a history of atopy, and in allergic people It is known to be one of the diseases caused by genetic factors and immune diseases because it is associated with allergic rhinitis, asthma, and other allergic diseases. In the treatment of atopic dermatitis, corticosteroids such as corticosteroids are expected to have high pharmacological effects. However, side effects of corticosteroids have become a problem. It is also well known that a dramatic aggravation of lesions occurs later. As a countermeasure for such side effects, nonsteroidal anti-inflammatory drugs or antihistamines, which do not contain hormones such as corticosteroids, are used, but since it is difficult to cure, there is an urgent need to develop an atopic dermatitis improving agent or treatment without side effects.

Recently, in order to reduce skin irritation caused by various chemicals, much attention has been focused on functional cosmetics having functions such as antioxidant, wrinkle relief, whitening, and itching relief obtained from natural extracts. For example, US Pat. No. 5,972,341 relates to the antiwrinkle effect of Commiphora mukul extract, and Japanese Patent Application Laid-open No. Hei 9-672662 relates to seaweed extract having hyaluroronidase inhibitory effect. Japanese Patent Application Laid-open No. Hei 10-85905 relates to the skin texture improvement effect of Fucoidan extracted from Wakame, and Japanese Patent Application Laid-open No. Hei 2-245087 relates to the antioxidant effect of the extract of Sargassum, Korea Patent No. 0431076 discloses a cosmetic composition for improving the healing of atopic skin, which contains white powder, jasminoide, echinacea and fermented soybean extract, and Korean Patent No. 0511494 extracts and compositions for treating atopic dermatitis, including sewage, leaf, and illite. Discussing about.

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In order to solve the problem of the skin, a lot of cosmetics using natural products or medicinal and edible mushroom extracts have recently been developed to reduce skin irritation caused by various chemicals. As a cosmetic composition using the extract of the mushroom known so far, Snow Cordyceps sinensis (Korean Patent No. 0340185), Situary Mushroom, Skirt Mushroom (Korean Patent No. 0681703, 0295623), Leaf Mushroom (Korean Patent No. 0438009), Shiitake Mushroom And the research on the anti-aging effect of these extracts has been continued.

Mushrooms used for medicinal and edible foods are numerous examples and kinds, mushrooms that are being used and studied as a cosmetic composition is still known as a part.

On the other hand, Morel mushroom ( Morckella esculenta ) is a mycorrhizal fungus that occurs near bushes, gardens, roadsides, especially firs and spruce in March-May, and is distributed worldwide in Korea and Japan. The cap of the fruiting body contains a spermatozoa and a side lobe. The body is composed of a cap and a sack, 6-12 cm in height. Gad is light yellow, broad egg-shaped, with basket-shaped grooves and soft meat. The sack is 4 ~ 5.5 cm long and the butterfly is 3 ~ 2.6 cm long, almost cylindrical, white or light yellowish white, and hollow inside. Asystole is formed inside the groove at the base of the lampshade, in which eight colorless oval spores are formed. Spore surface flat and flat with spore pattern white. Morel is edible but toxic, so boiled water should be discarded and cooked.

 It has not been determined whether such morel mushrooms have a whitening effect, skin irritation and atopic improvement as a skin external preparation.

Republic of Korea Patent No. 10-0438009 (cosmetic composition containing the extract of leaf mycelium mycelium) is a mycelium or mycelium of the leaf mushroom having an antioxidant effect and the effect of promoting the proliferation of skin cells, the production of collagen and the inhibitory effect of skin melanogenesis Although it described about the cosmetic composition containing the obtained extract, it does not describe the use of morel fruit body extract or morel mycelium culture medium. Republic of Korea Patent No. 10-0340185 (Cosmetic anti-wrinkle cosmetic composition containing an extract of Snow Cordyceps sinensis) has been described for a cosmetic composition excellent in preventing wrinkles by containing an extract of Snow Cordyceps sinensis, which includes morel fruit body extract or Morel mushroom mycelium culture medium It is not described with respect to using.

An object of the present invention is to provide a skin external preparation or cosmetic composition having a whitening, atopic improvement and skin irritant effect using a natural extract.

In addition, an object of the present invention is to provide a skin external preparation or cosmetic composition comprising a culture solution obtained from the culture of the higher fungus morel fruit body extract or morel mushroom mycelium as an active ingredient in order to solve the above problems. .

In addition, an object of the present invention is to provide a method for efficiently obtaining the morel fruit body extract or morel mycelia extract having a complexing effect of whitening, atopy and skin irritation.

In order to achieve the above object, the present invention provides a skin external preparation or cosmetic composition comprising a culture solution obtained from the culture of morel fruit body extract or morel mushroom mycelium as an active ingredient.

Hereinafter will be described in detail with respect to the problem solving means of the present invention.

The external preparation composition or cosmetic composition of the present invention is to provide a skin external preparation or cosmetic composition excellent in whitening, atopic dermatitis improvement, prevention or treatment and skin irritation effect, the culture solution obtained from the culture of morel fruit body extract or morel mycelium mycelium It is characterized by containing as an active ingredient.

Morel fruit body extract of the present invention can be obtained by extracting the morel fruit body, the type of morel fruit body is not limited to a specific kind can be used both natural or artificial cultured.

In addition, the extract of the morel fruit body extract of the present invention can be used a method commonly used in the art. For example, about 2 to 20 times, preferably about 2 to 5 times of water, methanol or ethanol, such as lower (C1-C4) alcohol, to the fruiting body powder obtained by lyophilization and grinding of the morel fruit body As a polar solvent or a mixed solvent thereof, extraction methods such as cold needle extraction, reflux cooling extraction, hot water extraction, ultrasonic extraction, and ultrahigh pressure extraction at 10 ° C to 30 ° C extraction temperature may be used.

In addition, the extract thus obtained may be preferably soluble in a polar solvent by filtration, concentration under reduced pressure, or lyophilization. For example, the obtained extract is filtered and then transferred to a concentration tank, concentrated under reduced pressure and freeze-dried at 50 ° C. or lower, and purified water, ethanol, butylene glycol, so as to contain 0.001-70.0 wt% of the reduced pressure concentrate and lyophilized product thus obtained. Extracts may be prepared using one or more solvents selected from propylene glycol.

In addition, the morel fruit body extract of the present invention may mean the polysaccharide itself contained in the extract obtained as an active ingredient.

On the other hand, the morel mushroom mycelium extract of the present invention is obtained by extracting the morel mushroom mycelium, the extraction method can be used a method commonly used in the art. For example, the extract may be a hot water extract of the mycelium powder obtained by lyophilization and grinding of the morel mushroom mycelium, or an organic solvent extract extracted using methanol, ethanol, butanol or a mixed solvent thereof.

On the other hand, the culture solution obtained from the culture of the morel mushroom mycelium can be used as it is, or dried (or lyophilized) can be used in powder form. Preferably, the culture solution may be used by filtration. Specifically, after the liquid culture of the morel mushroom mycelium, the mycelium is removed by centrifugation, the filtrate obtained by filtering the culture filtrate with a paper filter can be used. At this time, the culture is meant to include 'fermentation'.

In addition, the culture solution obtained from the culture of the morel mushroom mycelium extract or morel mycelium mycelium may be a polysaccharide extracted from the hot water extract, the organic solvent extract or the filtrate.

In addition, the culture solution obtained from the culture of the morel mushroom mycelium extract or morel mycelium mycelium of the present invention is preferably an extract of the morel mushroom mycelium using at least one animal or plant or herbal medicine as a substrate. Here, the animal or plant or herbal medicine is not particularly limited, and animals or plants or herbal medicines commonly used in the art to which the present invention pertains may be used as a substrate.

The culture solution obtained from the culture of the morel fruit body extract or morel mycelium mycelium extract or morel mycelium mycelium of the present invention obtained in this way is very excellent in whitening effect, anti-inflammatory effect on atopic dermatitis and skin irritant ability, so that the multifunctional skin external preparation or cosmetic It can be used in the composition.

On the other hand, the skin external preparation or cosmetic composition of the present invention preferably contains 0.001 to 90.0% by weight of the culture solution obtained from the culture of morel fruit body extract or morel mycelium mycelium, with less than 0.001% by weight If the effect is insignificant and exceeds 90.0% by weight, it is not economical because the efficiency is inferior to the material input amount.

On the other hand, the meaning of "included as an active ingredient" in the present invention means that the effect of the prevention and suppression of skin diseases from the external application for skin or cosmetic composition of the present invention, the extract of morel fruit body or morel in the external application for skin or cosmetic composition This means that the culture solution obtained from the culture of the mushroom mycelium is added, and that it is formulated in various forms by adding various components as sub-components for drug delivery and stabilization.

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On the other hand, the topical skin composition of the present invention is preferably a pharmaceutical composition, the formulation of the pharmaceutical composition, for example, as a warning agent (PLASTERS), lotion (LOTIONS), linings (LINIMENTS), liquids (LIQUIDS AND SOLUTIONS) , AEROSOLS, EXTRACTS, Ointment, FLUIDEXTRACTS, Emulsion, Emulsion, SUSPESIONS, CAPSULES, CREAMS, Soft or Hard Gelatin capsules, patches, sustained release agents.

On the other hand, the pharmaceutical composition of the present invention is a pharmacologically acceptable base; Vehicle; Excipients; Binders including starch, tragacanth gum, gelatin, molasses, polyvinyl alcohol, polyvinyl ether, polyvinyl pyrrolidone, hydroxypropyl cellulose, methyl cellulose, ethyl cellulose and carboxymethyl cellulose; Grinding agents including agar, starch, gelatin powder, sodium carboxymethyl cellulose, calcium carboxymethyl cellulose, crystalline cellulose, calcium carbonate, sodium bicarbonate and sodium alginate; Lubricants including magnesium stearate, talc and hydrogenated vegetable oils; And colorants and the like, and the carriers and excipients include lactose, glucose, sucrose, mannitol, potato starch, corn starch, calcium carbonate, calcium phosphate and cellulose. In addition to the above, additives such as adjuvants such as stabilizers, dissolution aids, transdermal absorption accelerators, and preservatives may be further added.

On the other hand, for many patients, the standard dosage of the topical skin composition can be varied by the individual characteristics of the patient, and a substantially skilled clinician would ideally administer the topical skin composition of the present invention for the patient. The most appropriate treatment strategy can be determined in terms of dosage and dosing schedule, eg, specific needs and the overall conditions of the patient. For the topical dermatological compositions of the invention, reference can be made to a number of references in determining the appropriate dosage.

In addition, the appropriate dosage of the external preparation composition for skin of the present invention may be generally determined in vitro or in an animal model. For example, appropriate concentrations may be determined by adding various concentrations of the topical skin composition of the invention to target cells in vitro.

On the other hand, the skin cosmetic composition of the present invention is preferably used for skin whitening, atopic skin improvement and skin irritation, the formulation of the cosmetic composition, for example, lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) type and water-in-oil (W / O) type of base cosmetic formulation; Color cosmetic formulations consisting of oil-in-water or water-in-oil makeup bases, foundations, skin covers, lipsticks, lip gloss, face powders, two-way cakes, eye shadows, cheek collars and eyebrow pencils; There is this.

According to the present invention, it is possible to obtain a yield of morel fruit body extract or morel mushroom mycelium culture medium that exhibits a complexing effect of whitening, atopic improvement and skin irritation, and can be used to prepare a multifunctional skin external preparation or cosmetic composition have.

Hereinafter, the content of the present invention will be described in more detail in the following examples. However, the scope of the present invention is not limited only to the following examples, but includes modifications of equivalent technical ideas.

Example  1: Preparation of Morel Fruit Body Extract

Morel (Morchella The fruiting body powder obtained by lyophilization and pulverization of the esculenta fruiting body was refluxed three times for 5 hours with an aqueous solution containing 70% (v / v) of ethanol, cooled, and filtered through Whatman # 5 filter paper. . The filtered extract was concentrated under reduced pressure and freeze-dried at 50 ° C. or lower, and then dissolved in a mixed solvent of purified water, ethanol, butylene glycol, and propylene glycol so as to contain 15% by weight of the obtained freeze-dried extract (hereinafter referred to as' mushroom fruiting body). Extracts').

Example  2: Morel Mushroom  Preparation of Mycelia Extract

Morel ( Morchella esculenta ) strains were isolated directly from fresh fruiting bodies, and the morel mushroom mycelium was isolated from yeast-wort agar medium (3 g of yeast extract, 3 g of wort, 3 g of glucose, 5 g of peptone, 20 g of agar, 1 of distilled water). Sloped in a test tube containing ℓ) was stored at 4 ℃ and used subcultured every month. After aseptic homogenization of the mycelium grown on the slope medium, it was prepared as a simple complex medium containing 3% glucose and 1.0% yeast extract and inoculated 5% (v / v) in a liquid medium adjusted to pH 5.5, The cells were incubated for 10 days under conditions of a temperature of 27 ° C., a rotational speed of 150 rpm, and an air flow rate of 1.5 vvm.

After completion of the culture, the mycelium was recovered by centrifugation, and extracted by the method described in Example 1 to prepare a mycelium extract (hereinafter, referred to as 'morel mushroom mycelium extract').

Example  3: Preparation of a Culture Solution Obtained from the Culture of Morel Mushroom Mycelia

Morel Mushroom ( Morchella) Used in the Experiment esculenta ) strains were isolated directly from fresh fruiting bodies, and the myrtle mycelium was isolated from yeast-wort agar medium (3 g of yeast extract, 3 g of wort, 10 g of glucose, 5 g of peptone, 20 g of agar, and 1 L of distilled water). Slope cultured in a test tube and stored at 4 ℃ and used subcultured every month. After aseptic homogenization of the mycelium grown on the slope medium, it was prepared as a simple complex medium containing 3% glucose and 1.0% yeast extract and inoculated 5% (v / v) in a liquid medium adjusted to pH 5.5, The cells were incubated for 10 days under conditions of a temperature of 27 ° C., a rotational speed of 150 rpm, and an air flow rate of 1.5 vvm.

After completion of the culture, the culture filtrate from which the mycelium was removed through centrifugation was filtered through a paper filter (watman no. 4) to obtain a filtrate (hereinafter, referred to as 'morel mushroom mycelia culture solution').

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Experimental Example 1 Measurement of Inhibitory Effect of Tyrosinase Activity

In Experimental Example 1, a well-known arbutin as a whitening agent was used as a comparative sample to confirm the inhibitory effect of tyrosinase activity of the Morel mushroom mycelium culture obtained in Example 3.

Tyrosinase is an enzyme that accelerates the oxidation process of a substance called tyrosine in vivo to produce melanin.

Tyrosinase was isolated and purified from mushrooms and purchased from Sigma. L-tyrosine, a substrate, was dissolved in 0.05 M sodium phosphate buffer (pH 6.8) to make a 0.1 mg / ml solution. It was used after adjusting to the concentration and dissolved. 0.5 ml of L-tyrosine solution was added to a test tube, and the extract of Morel fruit body and 0.5 ml of Morel mushroom mycelium culture solution was added thereto, and the mixture was left to stand at 37 ° C. for 10 minutes. Thereafter, 0.5 ml of 200 unit / ml tyrosinase was added and reacted at 37 ° C for 10 minutes. After the test tube containing the reaction solution was placed on ice and quenched to stop the reaction, the spectrophotometer measured absorbance at a wavelength of 475 nm. As a control, 0.5 ml of buffer solution was used instead of the morel fruit body extract and morel mycelium culture medium.

The tyrosinase activity inhibition rate of each sample was calculated according to Equation 1.

[Equation 1]

% Inhibition of tyrosinase activity = 100-[(comparative absorbance / control absorbance)] × 100

Sample Name Tyrosinase inhibition rate (%) Control 0 Morel Mushroom Mycelia Culture Medium 0.02% by weight 51 Morel Mushroom Mycelia Culture Weight 0.01% 41 Morel Mushroom Mycelium 0.005% by weight 35 Arbutin 0.2 wt% 54

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As a result of measuring the activity inhibitory effect of tyrosinase (Table 1), Morel mushroom mycelium culture showed a similar inhibition rate of tyrosinase activity to arbutin.
From the above results, it was possible to confirm the excellent tyrosinase activity inhibitory effect of the morel mushroom mycelium culture medium, based on this, the excellent whitening effect of the external preparation or cosmetic composition of the present invention.

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Example  4 and Comparative example  One : Morel Mushroom  Containing fruiting body extract Cosmetics  Produce

In Example 4 was prepared a cosmetic containing the extract of Morel mushroom fruit body obtained in Example 1.

Prepared cosmetics are in the form of a cream, the composition is shown in Table 2 below.

First, the phase b) recorded in the following Table 2 was heated and stored at 70 ° C., and the phase b) was added to the a) phase, followed by pre-emulsification. 1) was prepared.

Raw material Example 4 (% by weight) Comparative Example 1 (% by weight) end Stearyl alcohol 8 8 Stearic acid 2 2 Stearic cholesterol 2 2 Squalane 4 4 2-octyldodecyl alcohol 6 6 Polyoxyethylene (25 mol addition) alcohol ester 3 3 Glyceryl Monostearic Acid Aster 2 2 I Morel Fruiting Body Extract One - Propylene glycol 5 5 Purified water Until 100 Until 100

Example  5 and Comparative example  2 : Morel Mushroom  Containing mycelium culture Cosmetics  Produce

In Example 5 was prepared a cosmetic containing the morel mushroom mycelium culture obtained in Example 3.

Prepared cosmetics are in the form of a cream, the composition is shown in Table 3 below.

First, the phase b) recorded in the following Table 3 was heated and stored at 70 ° C., and the phase b) was added to the a) phase, and then emulsified uniformly with a homomixer and gradually cooled to cream (Example 4, Comparative Example). 2) was prepared.

Raw material Example 5 (% by weight) Comparative Example 2 (% by weight) end Stearyl alcohol 8 8 Stearic acid 2 2 Stearic cholesterol 2 2 Squalane 4 4 2-octyldodecyl alcohol 6 6 Polyoxyethylene (25 mol addition) alcohol ester 3 3 Glyceryl Monostearic Acid Aster 2 2 I Morel Mushroom Mycelia One - Propylene glycol 5 5 Purified water Until 100 Until 100

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Experimental Example 2: Determination of the improvement effect of cosmetics on atopic dermatitis

In Experimental Example 2, in order to compare and measure the improvement effect on atopic dermatitis of the cosmetics prepared in Examples 4 to 5 and Comparative Examples 1 to 2 was carried out as follows.

Thirty patients with atopic dermatitis aged 5 to 30 years who had atopic dermatitis for more than two years were examined to improve atopic dermatitis. To the same person, after washing every evening with Example 4 or Example 5 on the left hand and Comparative Example 1 or Comparative Example 2 on the right hand, it was applied to the skin once a day for 60 days and the degree of atopic dermatitis improvement was measured. The measurement was performed by sensory evaluation by survey. The results are shown in Tables 4 and 5 below.

very good good so so Example 4 80% (24 people) 20% (6 people) 0% Comparative Example 1 10% (3 people) 20% (6 people) 70% (21 people)

very good good so so Example 5 70% (21 people) 30% (9 persons) 0% Comparative Example 2 10% (3 people) 20% (6 people) 70% (21 people)

As a result of measuring the improvement effect on cosmetic atopic dermatitis (Table 4, Table 5), Example 3 containing morel fruit body extract and Example 4 containing morel mushroom mycelium culture medium is more atopic than Comparative Examples 1 to 2 It was confirmed that the skin improvement effect is excellent.

The atopic dermatitis improvement effect of the skin external preparation or cosmetic composition of this invention was confirmed from the said result.

Experimental Example 3: Determination of skin irritation effect by SLS

In the present experimental example, the stimulating alleviation effect of the cosmetics prepared in Examples 4 to 5 was evaluated by human patch experiment.

After mixing 1% SLS (sodium lauryl sulfate), which causes irritation to a general cosmetic prescription (cream, lotion, skin, essence) and the cosmetics of Example 4 or 5, patching for 24 hours, 48 hours, 72 hours, The effects of stimulation relaxation were evaluated based on the stimulus induction index.

FINN CHAMBER (FINLAND) was applied to the upper arm of 50 healthy men and women aged 20 to 50 with 0.3 mg of each product, and the acute irritation index was evaluated after 24 hours. After evaluation, the same amount of product was applied to the same site again and the delayed stimulation index after 48 and 72 hours was evaluated.

As a result of measuring the skin irritation-relieving effect by SLS, the area covered with SLS alone showed red stimulation to the skin after 24 hours. Example 5 did not cause any skin seizure even after 24 hours, 48 hours, 72 hours after patching.

From the above results, it was confirmed that the extract of morel fruit body and morel mycelium culture medium can reduce the skin irritation by the substrate (surfactant, fragrance, alcohol) that causes irritation when mixed with the cosmetic.

Example  6: Morel Mushroom  Preparation of lotion containing fruiting body extract

To 8 g of 95% ethanol, 0.05 g of polypyrrolidone, 0.1 g of oleyl alcohol, 0.2 g of polyoxyethylene monooleate, 0.2 g of fragrance, 0.1 g of paraoxybenzoic acid methyl ester, a small amount of antioxidant, and a small amount of pigment It was. 0.05 g of the morel fruit body extract obtained in Example 1, 5 g of glycerin was dissolved in 85.33 g of purified water, the mixture was added and stirred to prepare a lotion containing the morel fruit body extract.

Example  7: Morel Mushroom  Preparation of lotion containing mycelium culture

To 8 g of 95% ethanol, 0.05 g of polypyrrolidone, 0.1 g of oleyl alcohol, 0.2 g of polyoxyethylene monooleate, 0.2 g of fragrance, 0.1 g of paraoxybenzoic acid methyl ester, a small amount of antioxidant, and a small amount of pigment It was. 0.05 g of the mycelia mushroom mycelium culture solution obtained in Example 3, 5 g of glycerine was dissolved in 85.33 g of purified water, the mixed solution was added and stirred to prepare a lotion containing the mushroom mycelium culture solution.

Example  8: Morel Mushroom  Manufacture of latex containing fruiting body extract

1.2 g of cetyl alcohol, 10 g of squalane, 2 g of vaseline, 0.2 g of paraoxybenzoic acid ethyl ester, 1 g of glycerin monoesteralide, 1 g of polyoxyethylene (20 molar addition) monooleate and 0.1 g of fragrance at 70 ° C The mixture was heated and dissolved, and 0.5 g of the morel fruit body extract obtained in Example 1, 5 g of dipropylene glycol, 2 g of polyethylene glycol-1500, 0.2 g of triethanolamine, and 76.2 g of purified water were dissolved at 75 ° C. Both were emulsified by mixing, followed by cooling to prepare an oil-in-water (O / W) type emulsion.

Example  9: Morel Mushroom  Preparation of latex containing mycelium culture

1.2 g of cetyl alcohol, 10 g of squalane, 2 g of vaseline, 0.2 g of paraoxybenzoic acid ethyl ester, 1 g of glycerin monoesteralide, 1 g of polyoxyethylene (20 molar addition) monooleate and 0.1 g of fragrance at 70 ° C The mixture was dissolved by heating, and 0.5 g of the morel mushroom mycelium culture solution obtained in Example 3, 5 g of dipropylene glycol, 2 g of polyethylene glycol-1500, 0.2 g of triethanolamine, and 76.2 g of purified water were dissolved at 75 ° C. Both were emulsified by mixing, followed by cooling to prepare an oil-in-water (O / W) type emulsion.

Example  10: Morel Mushroom  Containing fruiting body extract Essence  Produce

To 5 g of 95% ethyl alcohol, 1.2 g of polyoxyethylene sorbitan monooleate, 0.3 g of chitulose, 0.2 g of sodium hyaluronate, 0.2 g of vitamin E-acetate, 0.2 g of sodium licorice, 0.1 g of paraoxybenzoic acid ethyl ester , 1 g of morel fruit body extract obtained in Example 1 and a suitable amount of pigment was mixed to prepare a cosmetic solution.

Example  11: Morel Mushroom  Containing mycelium culture Essence  Produce

To 5 g of 95% ethyl alcohol, 1.2 g of polyoxyethylene sorbitan monooleate, 0.3 g of chitulose, 0.2 g of sodium hyaluronate, 0.2 g of vitamin E-acetate, 0.2 g of sodium licorice, 0.1 g of paraoxybenzoic acid ethyl ester , 1 g of Morel mushroom mycelium culture medium obtained in Example 3 and a suitable amount of pigment was mixed to prepare a cosmetic solution.

Claims (18)

Pharmaceutical composition for the prevention or treatment of atopic dermatitis, characterized by containing morel fruit body extract or morel mycelia extract as an active ingredient.
A pharmaceutical composition for preventing or treating atopic dermatitis, comprising a culture solution obtained from the culture of the morel mushroom mycelium as an active ingredient.
The method according to any one of claims 1 to 2,
The pharmaceutical composition for preventing or treating atopic dermatitis,
A pharmaceutical composition for preventing or treating atopic dermatitis, characterized by containing from 0.001 to 90.0% by weight of the whole composition from the culture of morel fruit body extract or morel mushroom mycelium extract or morel mushroom mycelium.
Cosmetic composition characterized in that it contains morel fruit body extract or morel mycelia extract as an active ingredient.
A cosmetic composition comprising a culture solution obtained from the culture of morel mushroom mycelium as an active ingredient.
The method of claim 5,
The cosmetic composition,
Skin cosmetic composition for skin whitening.
The method according to any one of claims 4 or 5,
The cosmetic composition,
Skin cosmetic composition, characterized in that for improving atopic skin.
The method according to any one of claims 4 or 5,
The cosmetic composition,
Skin cosmetic composition, characterized in that for reducing skin irritation. delete delete delete delete delete delete delete delete delete delete