CN107164452A - A kind of detection method of morchella fungal bacterial strain cell age - Google Patents
A kind of detection method of morchella fungal bacterial strain cell age Download PDFInfo
- Publication number
- CN107164452A CN107164452A CN201710419500.2A CN201710419500A CN107164452A CN 107164452 A CN107164452 A CN 107164452A CN 201710419500 A CN201710419500 A CN 201710419500A CN 107164452 A CN107164452 A CN 107164452A
- Authority
- CN
- China
- Prior art keywords
- cell age
- flat board
- culture medium
- mycelial growth
- mycelia
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of detection method of morchella fungi cell age, detection method of the invention includes：Bacterial strain activation, flat board subculture are determined or U " types pipe is determined, cell age and mycelial growth rate are calculated and the step of interpretation of result; pass through the detection method of the present invention; can accurately, quickly judge the cell age of bacterial strain; so that it is determined that bacterial strain whether aging; provide and effectively refer to for screening, rejuvenation, culture, cultivation of bacterial strain etc., be that economic benefit is carried out in hickory chick industrial zone.
Description
Technical field
The present invention relates to edible mushroom field, and in particular to a kind of detection method of morchella fungal bacterial strain cell age, passes through
This method can effective detection morchella fungal bacterial strain whether aging.
Background technology
Hickory chick is a kind of rare edible mushroom, is able to large area plantation popularization, the sheep tripe of 2016 in China in recent years
Bacterium cultivated area has reached 24000 mu, is 3 times of the gross area in 2015, speedup is swift and violent.While this fast-developing along with
The impact that strain quality is caused to industry, every year because strain quality problem at least caused by 50% planting household damaged by great economy
Lose.
Among these, it is exactly that morchella fungi has the feature of quick aging, i.e. different strains the problem of a key
Cell age is widely different.If have selected the larger bacterial strain of cell age accidentally, it will cause significant impact to production.
The content of the invention
It is an object of the invention to provide a kind of detection method of morchella fungi cell age, pass through the detection method, energy
Enough accurate, the quick cell age for judging bacterial strain, so that it is determined that bacterial strain whether aging, be that follow-up bacterial strain screening, rejuvenation, culture etc. are carried
Effect reference is provided with, so as to carry out huge economic benefit for the cultivation industrial zone of hickory chick.
The technical scheme is that：
A kind of detection method of morchella fungi cell age, comprises the following steps：
1）Bacterial strain is activated
2）Flat board subculture is determined or U " types pipe is determined；
3）Cell age and mycelial growth rate are calculated；
4）Interpretation of result.
Wherein bacterial strain activation includes：Culture medium is prepared, prepared by activation and flat board；Specially：
The preparation of culture medium：The solid medium cultivated using suitable Morciiella Esculeuta Mycelia；
Bacterial strain is activated：It will be activated, after mycelia covers with test tube, treated in strain to be tested access solid medium test tube slant
With；
It is prepared by flat board：Rear plate sterilize aseptically, solid medium is poured into, it is standby after after culture medium cooled and solidified.
Flat board subculture is determined or the measure concrete operations of U " types pipe are：
Flat board subculture determination method：Flat board is sterilized, and culture medium is poured under aseptic condition, after culture medium is cooled down, in flat board on one side, away from
Inoculation inoculation block at the cm of isolated edge 0.5 ~ 1, and kind of a block is gently crimped with transfer needle, it is sticked on culture medium, in incubator
5 ~ 7 d are cultivated, the growing state of irregular observation bacterium colony during culture, and the position of fixed timing mark bacterium colony front end, it is used for
Mycelial growth rate is calculated, when bacterium colony nearly grows to the another side of flat board, with transfer needle towards mycelial growth direction, is cut
The cm of bacterium colony front end about 0.5 × 1.0 rectangle mycelia bar, and mycelia is kept towards forward direction switching such as new flat board side,
Mycelia is set to be always maintained at the growth conditions to a direction；
" U " type pipe determination method：U-tube two ends are stoppered with tampon, are used after sterilizing, and culture medium is poured into pipe, keep flat pipe
When, the thickness of culture medium is 0.6 ~ 0.8 mm；" U " type pipe two ends are kept flat upward, to culture medium cooled and solidified；By bacterium to be measured
Strain aseptically takes the inoculation block of 0.5 cm sizes to access one end of " U " type pipe, keeps flat and is placed in culture in constant incubator,
Periodically observed, check mycelial growth situation, and mark the position of bacterium colony front end, calculate mycelial growth rate；Treat mycelia length
To " U " type pipe the other end culture medium front end when, under aseptic condition, take bacterium colony front end, keep mycelial growth direction, be transferred to new
In " U " type pipe, continue to cultivate observation.
Cell age and the specific calculating standard of mycelial growth rate are：
The calculating of cell age：Counted from first generation inoculation time, until the speed of growth of strain to be tested is dropped rapidly between death
Timing definition be bacterial strain cell age, cell age unit is day, and the cell age of primary isolated original strain is defined as 0 d.Bacterial strain
Cell age it is longer, as potential business promotion be worth it is bigger.
The calculating of mycelial growth rate：Determine the distance of mycelial growth in the period, unit mm/h or cm/d.
Cell age criterion is：
Healthy and strong Morciiella Esculeuta Mycelia is on solid medium, and 23 DEG C of cultures, average speed can reach 0.35 ~ 0.55 mm/h.Before aging
Mycelial growth rate decline rapidly, can be reduced to 0.05 ~ 0.1 mm/h's by normal state in the time of one week or so
Growth conditions, until final aging growth arrest.
Compared with prior art, beneficial effects of the present invention are：Effective it can be cultivated by this criterion in hickory chick
The preceding quality to strain quality is detected, has been reached and has been avoided the edible mushroom strain inferior in cultivation production process, for production band
To endanger, there is not relevant report also in hickory chick cultivation field at present.
Brief description of the drawings
Fig. 1 flat band methods squamous subculture switching schematic diagram；
Fig. 2 " U " type tube method squamous subculture switching schematic diagram；
The cell age of five hickory chick kinds of Fig. 3 and long deadbeat are intended to.
Embodiment
Embodiment 1
A kind of detection method of morchella fungi cell age, comprises the following steps：
1）Bacterial strain is activated, including：Culture medium is prepared, prepared by activation and flat board；
The preparation of culture medium：It is suitable for the solid medium that Morciiella Esculeuta Mycelia is cultivated using PDA, CYM etc., it is complete with CYM
Exemplified by culture medium, constitute and be：The g of glucose 20, the g of peptone 2, the g of dusty yeast 2, the g of potassium dihydrogen phosphate 0.46, phosphoric acid hydrogen two
The g of potassium 1, the g of magnesium sulfate 0.5, the g of agar powder 20, water 1000 mL, pH are natural.
Bacterial strain is activated：Strain to be tested is accessed and activated in fresh sterilized solid medium test tube slant, is trained
23 ~ 25 DEG C of temperature is supported, 5 ~ 7 d are after mycelia covers with test tube for culture, used as the strain of subsequent detection.
It is prepared by flat board：The cm of diameter 9 flat board, is used after sterilizing, under aseptic condition, topples over 12 ~ 15 mL solid cultures
Base, it is standby after after culture medium cooled and solidified.
2）Flat board subculture is determined or U " types pipe is determined；
Flat board subculture determination method, using 9 cm or the flat board of 15 cm diameters, flat board is sterilized according to conventional disinfecting action,
The mm of thickness 3 ~ 4 CYM culture mediums are poured under aseptic condition, after culture medium cooling after flat board on one side, apart from edge 0.5 ~
Inoculation inoculation block at 1 cm distances, and kind of a block is gently crimped with transfer needle, it is that it is sticked on culture medium, covers the lid of flat board,
5 ~ 7 d are cultivated in 23 DEG C of constant incubator, the growing state of irregular observation bacterium colony during culture, and fixed timing mark
The position of bacterium colony front end, it is small with transfer needle when bacterium colony nearly grows to the another side of flat board for calculating mycelial growth rate
The heart towards mycelial growth direction, cut the lower cm of bacterium colony front end about 0.5 × 1.0 rectangle mycelia bar, and keep mycelia direction
Forward direction switching such as new flat board side, makes mycelia be always maintained at the growth conditions to a direction.
Towards same at U " type pipe determination methods, the mm of diameter 18 ~ 22 glass tube, long 40 ~ 50 cm, the cm of two ends 10
Fire 30 ~ 45 ° of bending in direction（As shown in Figure 2）.Two ends are stoppered with tampon, are used, will be melted after carrying out high pressure steam sterilization
Solid medium, when aseptically toppling over along one end of pipe as kept flat, pipe in " U " type pipe, culture medium
The mm of thickness about 0.6 ~ 0.8, " U " type pipe two ends are kept flat upward, until being used after culture medium cooled and solidified.By strain to be tested
Aseptically take the inoculation block of 0.5 cm sizes to access one end of " U " type pipe, keep flat be placed in training in constant incubator afterwards
Support, periodically observed, check mycelial growth situation, and mark the position of bacterium colony front end, calculate mycelial growth rate.Treat mycelia
When growing to the other end culture medium front end of " U " type pipe, aseptically, careful takes bacterium colony front end, keeps mycelial growth side
To being transferred in new " U " type pipe.Continue to cultivate observation.
3）Cell age and mycelial growth rate are calculated；
The calculating of cell age：Counted from first generation inoculation time, until the speed of growth of strain to be tested is dropped rapidly between death
Timing definition be bacterial strain cell age, cell age unit is day, and the cell age of primary isolated original strain is defined as 0 d.Bacterial strain
Cell age it is longer, as potential business promotion be worth it is bigger.
The calculating of mycelial growth rate：Determine the distance of mycelial growth in the period, unit mm/h or cm/d.
4）Interpretation of result.
Healthy and strong Morciiella Esculeuta Mycelia is on solid medium, and 23 DEG C of cultures, average speed can reach 0.35 ~ 0.55 mm/h.
Mycelial growth rate before aging declines rapidly, can be reduced to 0.05 ~ 0.1 by normal state in the time of one week or so
Mm/h growth conditions, until final aging growth arrest.
Described above is only the preferred embodiment of the present invention, to those skilled in the art, is not departing from this hair
On the premise of bright raw material, it can be suitably modified, these are improved also within protection scope of the present invention.
Claims (7)
1. a kind of detection method of morchella fungi cell age, it is characterised in that comprise the following steps：
1）Bacterial strain is activated
2）Flat board subculture is determined or U " types pipe is determined；
3）Cell age and mycelial growth rate are calculated；
4）Interpretation of result.
2. wherein bacterial strain according to claim 1 activates a kind of detection method of morchella fungi cell age, its feature exists
In bacterial strain activation includes：Culture medium is prepared, prepared by activation and flat board.
3. a kind of detection method of morchella fungi cell age according to claim 2, it is characterised in that
The preparation of culture medium：The solid medium cultivated using suitable Morciiella Esculeuta Mycelia；
Bacterial strain is activated：It will be activated, after mycelia covers with test tube, treated in strain to be tested access solid medium test tube slant
With；
It is prepared by flat board：Rear plate sterilize aseptically, solid medium is poured into, it is standby after after culture medium cooled and solidified.
4. a kind of detection method of morchella fungi cell age according to claim 1, it is characterised in that；
Flat board subculture is determined or the concrete operations of U " types pipe measure are：
Flat board subculture determination method：Flat board is sterilized, and culture medium is poured under aseptic condition, after culture medium is cooled down, in flat board on one side, away from
Inoculation inoculation block at the cm of isolated edge 0.5 ~ 1, and kind of a block is gently crimped with transfer needle, it is sticked on culture medium, in incubator
5 ~ 7 d are cultivated, the growing state of irregular observation bacterium colony during culture, and the position of fixed timing mark bacterium colony front end, it is used for
Mycelial growth rate is calculated, when bacterium colony nearly grows to the another side of flat board, with transfer needle towards mycelial growth direction, is cut
The cm of bacterium colony front end about 0.5 × 1.0 rectangle mycelia bar, and mycelia is kept towards forward direction switching such as new flat board side,
Mycelia is set to be always maintained at the growth conditions to a direction；
" U " type pipe determination method：U-tube two ends are stoppered with tampon, are used after sterilizing, and culture medium is poured into pipe, when keeping flat pipe,
The thickness of culture medium is 0.6 ~ 0.8 mm；" U " type pipe two ends are kept flat upward, to culture medium cooled and solidified；By strain to be tested
Aseptically take the inoculation block of 0.5 cm sizes to access one end of " U " type pipe, keep flat and be placed in culture in constant incubator, it is fixed
Phase is observed, and checks mycelial growth situation, and marks the position of bacterium colony front end, calculates mycelial growth rate；Treat that mycelia grows to
During the other end culture medium front end of " U " type pipe, under aseptic condition, bacterium colony front end is taken, mycelial growth direction is kept, is transferred to new
In " U " type pipe, continue to cultivate observation.
5. a kind of detection method of morchella fungi cell age according to claim 1, it is characterised in that；
Cell age and the specific calculating standard of mycelial growth rate are：
The calculating of cell age：Counted from first generation inoculation time, until the speed of growth of strain to be tested is dropped rapidly between death
Timing definition be bacterial strain cell age, cell age unit is day, and the cell age of primary isolated original strain is defined as 0 d；
The cell age of bacterial strain is longer, is worth as potential business promotion bigger；
The calculating of mycelial growth rate：Determine the distance of mycelial growth in the period, unit mm/h or cm/d.
6. a kind of detection method of morchella fungi cell age according to claim 1, it is characterised in that；
Cell age criterion is：Healthy and strong Morciiella Esculeuta Mycelia is on solid medium, 23 DEG C of cultures, and average speed can reach 0.35 ~
0.55 mm/h；Mycelial growth rate before aging declines rapidly, can be reduced in the time of one week or so by normal state
0.05 ~ 0.1 mm/h growth conditions, until final aging growth arrest.
7. a kind of detection method of morchella fungi cell age, it is characterised in that comprise the following steps：
1）Bacterial strain is activated, including：Culture medium is prepared, prepared by activation and flat board；
The preparation of culture medium：Use the solid medium for being suitable for Morciiella Esculeuta Mycelia cultivation；
Bacterial strain is activated：Strain to be tested is accessed and activated in fresh sterilized solid medium test tube slant, culture temperature
23 ~ 25 DEG C of degree, 5 ~ 7 d are after mycelia covers with test tube for culture, used as the strain of subsequent detection；
It is prepared by flat board：The cm of diameter 9 flat board, is used after sterilizing, under aseptic condition, is toppled over 12 ~ 15 mL solid mediums, is treated
It is standby after culture medium cooled and solidified；
2）Flat board subculture is determined or U " types pipe is determined；
Flat board subculture determination method, using 9 cm or the flat board of 15 cm diameters, flat board is sterilized according to conventional disinfecting action,
The mm of thickness 3 ~ 4 CYM culture mediums are poured under aseptic condition, culture medium composition is：The g of glucose 20, the g of peptone 2, ferment
The g of female powder 2, the g of potassium dihydrogen phosphate 0.46, the g of dipotassium hydrogen phosphate 1, the g of magnesium sulfate 0.5, the g of agar powder 20, the mL of water 1000,
PH is natural；
After after culture medium cooling, inoculation is inoculated with block at the cm distances of edge 0.5 ~ 1, and light with transfer needle in flat board on one side
Crimping kind block, is that it is sticked on culture medium, covers the lid of flat board, and 5 ~ 7 d, training are cultivated in 23 DEG C of constant incubator
The growing state of irregular observation bacterium colony during supporting, and the position of fixed timing mark bacterium colony front end, for calculating mycelial growth speed
Degree, when bacterium colony nearly grows to the another side of flat board, with transfer needle it is careful towards mycelial growth direction, cut before lower bacterium colony
About 0.5 × 1.0 cm rectangle mycelia bar is held, and keeps mycelia towards forward direction switching such as new flat board side, makes mycelia
It is always maintained at the growth conditions to a direction；
Towards same direction at U " type pipe determination methods, the mm of diameter 18 ~ 22 glass tube, long 40 ~ 50 cm, the cm of two ends 10
30 ~ 45 ° of bending is fired, two ends are stoppered with tampon, used after carrying out high pressure steam sterilization；By the solid medium of thawing,
When toppling under aseptic condition along one end of pipe as kept flat, pipe in " U " type pipe, the thickness of culture medium is 0.6 ~ 0.8
Mm, " U " type pipe two ends are kept flat upward, until being used after culture medium cooled and solidified；Strain to be tested is aseptically taken 0.5
The inoculation block of cm sizes accesses one end of " U " type pipe, keeps flat be placed in culture in constant incubator afterwards, periodically observed, examined
Mycelial growth situation is looked into, and marks the position of bacterium colony front end, mycelial growth rate is calculated；Treat that mycelia grows to the another of " U " type pipe
When holding culture medium front end, aseptically, careful takes bacterium colony front end, keeps mycelial growth direction, is transferred to new " U " type pipe
It is interior；Continue to cultivate observation；
3）Cell age and mycelial growth rate are calculated；
The calculating of cell age：Counted from first generation inoculation time, until the speed of growth of strain to be tested is dropped rapidly between death
Timing definition be bacterial strain cell age, cell age unit is day, and the cell age of primary isolated original strain is defined as 0 d；
The cell age of bacterial strain is longer, is worth as potential business promotion bigger；
The calculating of mycelial growth rate：Determine the distance of mycelial growth in the period, unit mm/h or cm/d；
4）Interpretation of result
Healthy and strong Morciiella Esculeuta Mycelia is on solid medium, and 23 DEG C of cultures, average speed reaches 0.35 ~ 0.55 mm/h；Before aging
Mycelial growth rate declines rapidly, and in the time of one week or so, 0.05 ~ 0.1 mm/h growth is reduced to by normal state
State, until final aging growth arrest.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710419500.2A CN107164452A (en) | 2017-06-06 | 2017-06-06 | A kind of detection method of morchella fungal bacterial strain cell age |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710419500.2A CN107164452A (en) | 2017-06-06 | 2017-06-06 | A kind of detection method of morchella fungal bacterial strain cell age |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107164452A true CN107164452A (en) | 2017-09-15 |
Family
ID=59824522
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710419500.2A Pending CN107164452A (en) | 2017-06-06 | 2017-06-06 | A kind of detection method of morchella fungal bacterial strain cell age |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107164452A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103141302A (en) * | 2013-03-19 | 2013-06-12 | 中国科学院昆明植物研究所 | Production method for morchella importuna cultivars |
| KR20140081137A (en) * | 2012-12-21 | 2014-07-01 | 주식회사 코씨드바이오팜 | A skin-care agent containig Morchella esculenta fruit body extract or Morchella esculenta mycelium extract |
-
2017
- 2017-06-06 CN CN201710419500.2A patent/CN107164452A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20140081137A (en) * | 2012-12-21 | 2014-07-01 | 주식회사 코씨드바이오팜 | A skin-care agent containig Morchella esculenta fruit body extract or Morchella esculenta mycelium extract |
| CN103141302A (en) * | 2013-03-19 | 2013-06-12 | 中国科学院昆明植物研究所 | Production method for morchella importuna cultivars |
Non-Patent Citations (4)
| Title |
|---|
| GULER PERIHAN等: "Cultural Characteristics of Morchella esculenta Mycelium on Some Nutrients", 《TURKISH JOURNAL OF BIOLOGY》 * |
| 刘伟等: "高羊肚菌菌株老化过程形态学研究", 《中国菌物学会第六届会员代表大会（2014年学术年会）暨贵州省食用菌产业发展高峰论坛会议摘要》 * |
| 张甫安等: "《食用菌制种指南 第2版》", 30 November 1992, 上海科学技术出版社 * |
| 王继鹏: "菌龄对核盘菌致病性的影响及植物抗核盘菌分子机制", 《中国博士学位论文全文数据库 农业科技辑》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104388329B (en) | A kind of application of Lactobacillus plantarum degradation biological amine in wine production | |
| CN102978132A (en) | Microorganism plant vaccine capable of controlling tomato bacterial wilt disease | |
| CN104988082A (en) | Debaryomyces hansenii strain and applications thereof | |
| CN103224897A (en) | Bacillussubtilis for tobacco black shank prevention and control | |
| Roukas et al. | Influence of some trace metals and stimulants on citric acid production from brewery wastes by Aspergillus niger | |
| CN112481350A (en) | Composite culture medium for rapidly detecting total number of moulds and yeasts and preparation method thereof | |
| CN105331672A (en) | Culture medium for detecting ethanol-tolerance contaminant microorganism in yellow rice wine, and application of culture medium | |
| Le Ruyet et al. | Characterization of Clostridium thermolacticum sp. nov., a hydrolytic thermophilic anaerobe producing high amounts of lactate | |
| McFeeters et al. | Malic acid degradation and brined cucumber bloating | |
| Walter et al. | Assessment of antibiotic resistance from long-term bacterial exposure to antibiotics commonly used in fuel ethanol production | |
| CN100352914C (en) | Vacuum freeze-dried product of antagonist bacteria C.laurentii of yeast and its preparation method | |
| CN107164452A (en) | A kind of detection method of morchella fungal bacterial strain cell age | |
| CN103789230B (en) | Biocontrol strain BS71 and the application in control farm crop aflatoxin contamination thereof | |
| CN103614306A (en) | Yeast strain and applications thereof | |
| CN105316427A (en) | Fluorescent quantitative PCR detection kit for quickly detecting pythium aphanidermatum | |
| CN106350467B (en) | Reduce the bacterial strain and screening technique, cultural method and application of nicotine in tobacco leaf | |
| CN102559843B (en) | Method for observing infection process of citrus canker pathogens in real time | |
| CN102146348A (en) | Homoserinelactone-producing acinetobacter calcoaceticus and application thereof | |
| CN102232358A (en) | Detoxification method for excised stem segment of grape | |
| CN104651339B (en) | The culture medium and its fermentation process of microvesicle Pseudomonas fermenting and producing algin catenase | |
| Mohammadi et al. | Investigation into seasonal effect and browning inhibitor on callus regeneration of seedless barberry (Berberis vulgaris var. asperma) | |
| CN106434838A (en) | Selective beer spoilage bacterium culture medium and application thereof | |
| CN106497851A (en) | Promote the culture medium of bacillus stearothermophilus fast germination | |
| CN109097435A (en) | A method of screening malaga carbohydrate oxidase strain from air | |
| CN105648028B (en) | Method for identifying resistance of Java black rot of sweet potatoes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170915 |