KR102073943B1 - A skin-care agent or cosmetics containig Lyophyllum ulmarium fruit body extract or Lyophyllum ulmarium mycelium extract - Google Patents

Abstract

The present invention relates to a skin external preparation or a cosmetic composition containing a culture solution obtained from the culture of the mandala mushroom fruiting body extract or mandrel mushroom mycelium. It relates to a functional skin external preparation or cosmetic composition excellent in whitening effect, atopy improving effect and skin irritation relaxing effect, characterized by containing ~ 90.0% by weight.

Description

A skin-care agent or cosmetics containig Lyophyllum ulmarium fruit body extract or Lyophyllum ulmarium mycelium extract}

The present invention relates to a skin external preparation or a cosmetic composition containing a mandala mushroom fruiting body extract or a mandala mushroom mycelium extract, and specifically, a culture liquid obtained from the culture of a filtrate obtained from a mandala mushroom fruiting body extract or a mandala mushroom mycelium. It relates to a skin external preparation or cosmetic composition containing.

Melanin is converted from tyrosine to dopa, dopaquinone by the action of tyrosinase present in pigment cells, and is generated via dopachrome. Melanin is present in the skin to protect the body from ultraviolet rays and other important functions in the body's hormone secretion control. However, when melanin is overproduced, it is known to play a significant role in forming blemishes, freckles, etc., promoting skin aging, and causing skin cancer, and research and development for preventing melanin overproduction are being actively conducted. Ascorbic acid (JP-A 4-9320), hydroquinone (JP-A 6-192062), kojic acid (JP-A-56-7710), arbutin (JP-A 4-93150), and plant extracts have already inhibited tyrosinase. It is used as a whitening cosmetic due to its activity, but its stability in cosmetic formulations is poor, and it is degraded and colored, odors occur, its efficacy and effect on the biological level are unclear, or its safety is a problem, and its use is restricted. to be.
On the other hand, atopic dermatitis is a representative disease in people with atopic dermatitis. Although the cause of atopic dermatitis has not been clarified yet, 70% of patients with atopic dermatitis have a history of atopic dermatitis in people with allergic predisposition. It is known as one of the diseases caused by genetic factors or immune diseases because it is associated with allergic rhinitis, asthma, and other allergic diseases. In the treatment of atopic dermatitis, corticosteroids such as corticosteroids are expected to have high pharmacological effects. However, side effects of corticosteroids have become a problem, and rebound (suspension of use of corticosteroids) is stopped. It is also well known that a dramatic aggravation of lesions occurs later. As a countermeasure for such side effects, nonsteroidal anti-inflammatory agents or antihistamines, which do not contain hormones such as corticosteroids, are used, but since it is difficult to cure, there is an urgent need to develop an atopic dermatitis improving agent or treatment without side effects.

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Recently, in order to reduce skin irritation caused by various chemicals, much attention has been focused on functional cosmetics (Functional cosmetics) having functions such as antioxidant, wrinkle relief, whitening, and itching relief obtained from natural extracts. For example, US Pat. No. 5,972,341 relates to the antiwrinkle effect of Commiphora mukul extract, and Japanese Patent Application Laid-open No. Hei 9-672662 relates to seaweed extract having hyaluroronidase inhibitory effect. Japanese Patent Application Laid-open No. Hei 10-85905 relates to the skin texture improvement effect of Fucoidan extracted from Wakame, and Japanese Patent Application Laid-open No. Hei 2-245087 relates to the antioxidant effect of the extract of Sargassum, Korea Patent No. 0431076 discloses a cosmetic composition for improving the healing of atopic skin, which contains white powder, jasminoide, echinacea and fermented soybean extract, and Korean Patent No. 0511494 extracts and compositions for treating atopic dermatitis, including sewage, leaf, and illite. Discussing about.

In order to solve this problem of skin, cosmetics using natural products or medicinal and edible mushroom extracts have recently been developed to reduce skin irritation caused by various chemicals. Cosmetic compositions using extracts of mushrooms known to date include Snow Cordyceps sinensis (Korean Patent No. 0340185), Situary Mushroom, Skirt Mushroom (Korean Patent No. 0681703, 0295623), Leaf Mushroom (Korean Patent No. 0438009), Shiitake Mushroom And the research on the anti-aging effect of these extracts has been continued.

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Mushrooms used for medicinal and edible foods are numerous examples and kinds, but mushrooms that are used and studied as a cosmetic composition are still known as a part.

On the other hand, Lyophyllum ulmarium is a fungus of the genus Pleurotus eryngii matsutake , which occurs as a bundle of hardwood dead trees or stumps in autumn. In the early stage of development, round button or hemispherical, flat when mature, and the surface of the initial lampshade is dark creamy and gradually fades as it grows. In particular, when dried, the surface of the lampshade is divided to form a turtle-like pattern, and the meat is thick but soft and broken well, and is used as a food ingredient. Mandang mushrooms are effective in dieting because they contain polysaccharide proteins that promote the release of cholesterol, inhibit cholesterol synthesis in the liver, and reduce fat.

 It is not known at all that this white stranded mushroom has a whitening effect, atopy improving effect and skin irritation relaxing effect.

Republic of Korea Patent No. 10-0438009 (cosmetic composition containing the extract of leaf mycelium mycelium) is a mycelium or mycelium of the leaf mushroom having an antioxidant effect and the effect of promoting the proliferation of skin cells, the production of collagen and the inhibitory effect of skin melanogenesis Although the cosmetic composition containing the obtained extract has been described, it has not been described in terms of using the mandala mushroom fruiting body extract or the mandrel mushroom mycelium culture. Republic of Korea Patent No. 10-0340185 (Anti-wrinkle cosmetic composition containing a snow Cordyceps sinensis extract) has been described for a cosmetic composition excellent in the prevention of wrinkles by containing an extract of Snow Cordyceps sinensis, but this is the fruiting body extract or Mandang mushroom There is no description on the use of mycelium culture.

The present invention is to provide a skin external preparation or cosmetic composition having a whitening, atopic improvement and skin irritant effect using a natural extract.

In another aspect, the present invention to provide a skin external preparation or cosmetic composition comprising a culture solution obtained from the culture medium of the higher fungus mandala mushroom fruiting body extract or mandala mushroom mycelium as an active ingredient. There is this.

In addition, an object of the present invention is to provide a method for efficiently obtaining the mandala mushroom fruiting body extract or mycelium extract having a complexing effect of whitening, atopy improvement and skin irritation.

In order to achieve the above object, the present invention provides a skin external preparation or cosmetic composition comprising a culture solution obtained from the cultivation of the mandala mushroom fruiting body extract or mandala mushroom mycelium as an active ingredient.
Hereinafter will be described in detail with respect to the problem solving means of the present invention.
The external preparations or cosmetic compositions of the present invention from the cultivation of the fruiting body extract of the mandala mushroom or mandala mushroom mycelium to provide a skin external preparation or cosmetic composition excellent in whitening effect, atopic dermatitis improvement / prevention or treatment effect and skin irritation effect It is characterized by containing the obtained culture solution as an active ingredient.
Mandang mushroom fruiting body extract of the present invention can be obtained by extracting the mandrel mushroom fruiting body, where the type of fruiting body is not limited to a specific kind can be used both natural or artificial cultured.
In addition, the extraction method of the mandala mushroom fruiting body extract of the present invention can be used a method commonly used in the art to which the present invention belongs. For example, about 2 to 20 times, preferably about 2 to 5 times lower water (C1-C4) alcohol, such as methanol, ethanol, or the like, from the fruiting body powder obtained by lyophilization and grinding of the stranded fruiting body As a polar solvent or a mixed solvent thereof, extraction methods such as cold needle extraction, reflux cooling extraction, hot water extraction, ultrasonic extraction, ultrahigh pressure extraction, and the like may be used at an extraction temperature of 10 ° C to 30 ° C.
In addition, the extract thus obtained may be preferably soluble in a polar solvent by filtration, concentration under reduced pressure or lyophilization. For example, the obtained extract is filtered and then transferred to a concentration tank, concentrated under reduced pressure and freeze-dried at 50 ° C. or lower, and purified water, ethanol, butylene glycol, so as to contain 0.001 to 70.0 wt% of the reduced pressure concentrate and the freeze-dried product thus obtained. Extracts may be prepared using one or more solvents selected from propylene glycol.
In addition, the mandala mushroom fruiting body extract of the present invention may mean the polysaccharide itself contained as an active ingredient in the extract thus obtained.
On the other hand, the mandala mycelium mycelium extract of the present invention is obtained by extracting the mandala mycelium mycelium, the extraction method can be used a method commonly used in the art. For example, it may be a hot water extract of the mycelium powder obtained by freeze-drying and grinding the mycium mushroom mycelium, or an organic solvent extract extracted using methanol, ethanol, butanol or a mixed solvent thereof.
On the other hand, the culture solution obtained from the culture of the mandrel mushroom mycelium can be used as it is, or dried (or lyophilized) can be used in powder form. Preferably, the culture solution may be filtered. Specifically, after liquid culture of the mandrel mushroom mycelium, the mycelium is removed by centrifugation, and the filtrate obtained by filtering the culture filtrate with a paper filter can be used. At this time, the culture is meant to include 'fermentation'.
In addition, the culture medium obtained from the cultivation of the mandala mycelium mycelium extract or mandang mushroom mycelium of the present invention may be a polysaccharide extracted from the hot water extract, the organic solvent extract or the filtrate.
In addition, it is preferable that the culture solution obtained from the cultivation of the mandala mycelium mycelium extract or mandala mushroom mycelium of the present invention is an extract of the mandala mushroom mycelium using at least one animal or plant or herbal medicine as a substrate. Here, the animal or plant or herbal medicine is not particularly limited, and may be used as a substrate an animal or plant or herbal medicine commonly used in the art.
The culture solution obtained from the cultivation of the barley mushroom fruiting body extract or the barley mushroom mycelium extract or the barley mushroom mycelium of the present invention thus obtained is very multifunctional due to its excellent whitening effect, anti-inflammatory effect against atopic dermatitis and skin irritation effect. It can be used for external skin preparations or cosmetic compositions.
On the other hand, the skin external preparation or cosmetic composition of the present invention preferably contains 0.001 to 90.0% by weight of the culture solution obtained from the cultivation of the fruiting body extract or mandrel mushroom mycelium extract or mandrel mushroom mycelium on the whole composition, 0.001% by weight If it is included less than the skin improvement effect is insignificant, if it exceeds 90.0% by weight is less efficient compared to the amount of material input because it is not economical.
On the other hand, the meaning of "included as an active ingredient" in the present invention to the extent that can exhibit the effect of preventing and inhibiting skin diseases from the external preparation or cosmetic composition of the present invention, the extract of the fruiting body of the barley mushroom fruit body or to the external preparation or cosmetic composition This means that the culture solution obtained from the cultivation of the mandrel mycelium mycelium extract or the mandrel mushroom mycelium is added, and it means that it is formulated in various forms by adding various components as sub-components for drug delivery and stabilization. .
On the other hand, the topical skin composition of the present invention is preferably a pharmaceutical composition, for example, the formulation of the pharmaceutical composition as a warning agent (PLASTERS), lotion (LOTIONS), linings (LINIMENTS), liquids (LIQUIDS AND SOLUTIONS) , AEROSOLS, EXTRACTS, Ointments, FLUIDEXTRACTS, Emulsions, Suspensions, SUSPESIONS, CAPSULES, CREAMS, Soft or Hard Gelatin capsules, patches, sustained release agents.
On the other hand, the pharmaceutical composition of the present invention is a pharmacologically acceptable base; Vehicle; Excipients; Binders including starch, tragacanth gum, gelatin, molasses, polyvinyl alcohol, polyvinyl ether, polyvinyl pyrrolidone, hydroxypropyl cellulose, methyl cellulose, ethyl cellulose and carboxymethyl cellulose; Grinding agents including agar, starch, gelatin powder, sodium carboxymethyl cellulose, calcium carboxymethyl cellulose, crystalline cellulose, calcium carbonate, sodium bicarbonate and sodium alginate; Lubricants including magnesium stearate, talc and hydrogenated vegetable oils; And colorants and the like, and the carriers and excipients include lactose, glucose, sucrose, mannitol, potato starch, corn starch, calcium carbonate, calcium phosphate and cellulose. In addition to the above, additives such as adjuvants such as stabilizers, dissolution aids, transdermal absorption accelerators, and preservatives may be further added.
On the other hand, for many patients, the standard dosage of the topical skin composition can be varied by the individual characteristics of the patient, and a substantially skilled clinician would ideally administer the topical skin composition of the present invention for the patient. The most appropriate treatment strategy can be determined in terms of dosage and dosing regime, eg, specific needs and overall conditions of the patient. For the topical dermatological compositions of the present invention, numerous references can be consulted for determining appropriate dosages.
In addition, the appropriate dosage of the external preparation composition for skin of the present invention may be generally determined in vitro or in an animal model. For example, the appropriate dosage may be determined by adding various concentrations of the topical skin composition of the invention to target cells in vitro.
On the other hand, the pharmaceutical composition is preferably used for the prevention or treatment of atopy.
On the other hand, the cosmetic composition of the present invention is preferably used for skin whitening, atopic skin improvement and skin irritation, but the formulation of the cosmetic composition, for example, lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / Basic cosmetic formulations consisting of W) and water-in-oil (W / O) types; Color cosmetic formulation consisting of oil-in-water or water-in-oil makeup base, foundation, skin cover, lipstick, lip gloss, face powder, two-way cake, eye shadow, cheek color and eyebrow pencils; There is this.

According to the present invention, it is possible to obtain a good yield of the fruiting body extract or mandrel mushroom mycelium culture medium which exhibits the complexing effect of whitening, atopy improvement and skin irritation, using this to prepare a multifunctional skin external preparation or cosmetic composition can do.

Hereinafter, the content of the present invention will be described in more detail in the following examples. However, the scope of the present invention is not limited to the following examples, but includes modifications of equivalent technical ideas.
Example 1: Preparation of fruiting fruit extract

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The fruiting body powder obtained by freeze-drying and grinding the fruiting body of Lyophyllum ulmarium was refluxed and refrigerated three times for 5 hours with an aqueous solution containing 70% (v / v) of ethanol. Filter by 5 filter paper. The filtered extract was concentrated under reduced pressure and freeze-dried at 50 ° C. or lower, and then dissolved in a mixed solvent of purified water, ethanol, butylene glycol and propylene glycol so as to contain 15% by weight of the obtained freeze-dried extract. Fruiting body extract ”.
Example 2: Preparation of Pleurotus eryngii mycelia extract
Lyophyllum ulmarium strains used in the experiments were isolated directly from fresh fruiting bodies, and the mycelia were isolated from yeast-wort agar medium (3 g of yeast extract, 3 g of wort, 10 g of glucose, 5 g of peptone, 20 g of agar, 1 L of distilled water) was inclined in a test tube, stored at 4 ° C and subcultured every month. After aseptic homogenization of the mycelium grown on the slope medium, it was prepared as a simple complex medium containing 3% glucose and 1.0% yeast extract and inoculated 5% (v / v) in a liquid medium adjusted to pH 5.5, The culture was carried out for 10 days under conditions of a temperature of 27 ° C., a rotational speed of 150 rpm, and an aeration rate of 1.5 vvm.
After completion of the culture, the mycelium was recovered by centrifugation, and then extracted by the method described in Example 1 to prepare a mycelium extract (hereinafter referred to as 'mandala mushroom mycelium extract').
Example 3: Preparation of a Culture Solution Obtained from the Culture of Pleurotus eryngii Mycelia
Lyophyllum ulmarium strains used in the experiments were isolated directly from fresh fruiting bodies, and the mycelia were isolated from yeast-wort agar medium (3 g of yeast extract, 3 g of wort, 10 g of glucose, 5 g of peptone, 20 g of agar, 1 L of distilled water) was inclined in a test tube, stored at 4 ° C and subcultured every month. After aseptic homogenization of the mycelium grown on the slope medium, it was prepared as a simple complex medium containing 3% glucose and 1.0% yeast extract and inoculated 5% (v / v) in a liquid medium adjusted to pH 5.5, The culture was carried out for 10 days under conditions of a temperature of 27 ° C., a rotational speed of 150 rpm, and an aeration rate of 1.5 vvm.
After completion of the culture, the culture filtrate from which the mycelium was removed by centrifugation was filtered through a paper filter (watman no. 4) to obtain a filtrate (hereinafter, referred to as 'mantle mycelia culture medium').

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Experimental Example 1 Measurement of Inhibitory Effect of Tyrosinase Activity

In Experimental Example 1, arbutin, which is well known as a whitening agent, was used as a comparative sample in order to confirm the inhibitory effect of tyrosinase activity of the mandala mycelium culture medium obtained in Example 1.

Tyrosinase is an enzyme that promotes the oxidation process of a substance called tyrosine in vivo to produce melanin.

Tyrosinase was isolated and purified from mushrooms and purchased from Sigma. L-tyrosine, a substrate, was dissolved in 0.05 M sodium phosphate buffer (pH 6.8) to make a 0.1 mg / ml solution, and the mandala mushroom fruit body extract and the mandala mushroom mycelium culture solution were each 0.05 M sodium phosphate buffer (pH 6.8). It was used after adjusting to the appropriate concentration to dissolve. 0.5 ml of L-tyrosine solution was placed in a test tube, and the extract of Mannfold mushroom fruiting body and 0.5 ml of Myrtle mushroom mycelium culture solution was added thereto, and the mixture was left to stand at 37 ° C. for 10 minutes. Thereafter, 0.5 ml of 200 unit / ml tyrosinase was added and reacted at 37 ° C for 10 minutes. After the test tube containing the reaction solution was placed on ice and quenched to stop the reaction, the spectrophotometer measured absorbance at a wavelength of 475 nm. As a control, 0.5 ml of buffer solution was used in place of the mandala mushroom fruiting body extract and the mandrel mushroom mycelium culture medium.

The tyrosinase activity inhibition rate of each sample was calculated according to Equation 1.

[Equation 1]

% Inhibition of tyrosinase activity = 100-[(comparative absorbance / control absorbance)] × 100

Sample name Tyrosinase inhibition rate (%) Control 0 Mandala Mushroom Mycelia Culture Medium 0.02% by weight 51 0.01% by weight of Mycelial mycelia culture medium 41 0.005% by weight of Mycelial mycelia culture medium 35 Arbutin 0.2 wt% 54

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As a result of measuring the activity inhibitory effect of tyrosinase (Table 1), all of the fungus mycelium cultures showed similar inhibition rates of tyrosinase activity as Arbutin.

From the above results, it was possible to confirm the excellent tyrosinase activity inhibitory effect of the mandala mushroom fruiting body extract and the mandrel mushroom mycelium culture medium, based on the excellent whitening effect of the external preparation or cosmetic composition of the present invention.
Example 4 and Comparative Example 1 Preparation of Cosmetics Containing Fruit Extract
In the present Example 4 was prepared a cosmetic containing the extract of the fruit strand Mannchi mushroom obtained in Example 1.
Prepared cosmetics are in the form of a cream, the composition is shown in Table 2 below.
First, the phase b) recorded in the following Table 2 was heated and stored at 70 ° C., and the phase b) was added to the a) phase, and then emulsified uniformly with a homomixer and gradually cooled to a cream (Example 3, Comparative Example). 1) was prepared.
Raw material Example 4 (% by weight) Comparative Example 1 (% by weight) end Stearyl alcohol 8 8 Stearic acid 2 2 Stearic cholesterol 2 2 Squalane 4 4 2-octyldodecyl alcohol 6 6 Polyoxyethylene (25 mol addition) alcohol ester 3 3 Glyceryl Monostearic Acid Aster 2 2 I Mandala Mushroom Fruiting Body Extract One - Propylene glycol 5 5 Purified water Until 100 Until 100
Example 5 and Comparative Example 2: Preparation of cosmetics containing the mandrel mycelium mycelium culture
In Example 5 was prepared a cosmetic containing the mandrel mycelium mycelium culture medium obtained in Example 3.
Prepared cosmetics are in the form of a cream, the composition is shown in Table 3 below.
First, the phase b) recorded in Table 3 was heated and stored at 70 ° C., and the phase b) was added to the phase a) to pre-emulsify and then uniformly emulsified with a homomixer and gradually cooled to cream (Example 4, Comparative Example 2) was prepared.
Raw material Example 5 (% by weight) Comparative Example 2 (% by weight) end Stearyl alcohol 8 8 Stearic acid 2 2 Stearic cholesterol 2 2 Squalane 4 4 2-octyldodecyl alcohol 6 6 Polyoxyethylene (25 mol addition) alcohol ester 3 3 Glyceryl Monostearic Acid Aster 2 2 I Mandala Mushroom Mycelia Culture Solution One - Propylene glycol 5 5 Purified water Until 100 Until 100
Experimental Example 2: Determination of the improvement effect of cosmetics on atopic dermatitis
In Experimental Example 2, a clinical experiment was carried out as follows to compare and measure the improvement effect on atopic dermatitis of the cosmetics prepared in Examples 4 to 5 and Comparative Examples 1 to 2.
Thirty patients with atopic dermatitis aged 5 to 30 years who had atopic dermatitis for more than two years were examined to improve atopic dermatitis. To the same person, after washing every evening with Example 4 or Example 5 on the left hand and Comparative Example 1 or Comparative Example 2 on the right hand, the skin was applied once a day for 60 days and the degree of atopic dermatitis improvement was measured. The measurement was performed by sensory evaluation by survey. The results are shown in Tables 4 and 5 below.
very good good so so Example 4 80% (24 people) 20% (6 people) 0% Comparative Example 1 10% (3 people) 20% (6 people) 70% (21 people)
very good good so so Example 5 70% (21 people) 30% (9 persons) 0% Comparative Example 2 10% (3 people) 20% (6 people) 70% (21 people)
As a result of measuring the improvement effect on cosmetic atopic dermatitis (Table 3, Table 4), Example 3 containing the mandala mushroom fruiting body extract and Example 4 containing the mandala mushroom mycelium culture medium than Comparative Examples 1 and 2 It was confirmed that the atopic skin improvement effect is excellent.
The atopic dermatitis improvement effect of the skin external preparation or cosmetic composition of this invention was confirmed from the said result.
Experimental Example 3: Determination of skin irritation effect by SLS
In Experimental Example 3, the stimulating alleviation effect of the cosmetics prepared in Examples 4 to 5 was evaluated by human patch experiment.
After mixing 1% SLS (sodium lauryl sulfate), which causes irritation to a general cosmetic prescription (cream, lotion, skin, essence) and the cosmetics of Example 4 or 5, patching for 24 hours, 48 hours, 72 hours, The effects of stimulation relaxation were evaluated based on the stimulus induction index.
FINN CHAMBER (FINLAND) was used to patch 0.3 mg of the product on the upper arm of 50 healthy men and women aged 20 to 50 years, and the acute irritation index was evaluated after 24 hours. After evaluation, the same amount of product was applied to the same site again, and the delayed stimulation index after 48 and 72 hours was evaluated.
As a result of measuring the skin irritation-relieving effect by SLS, the area covered with SLS alone showed red stimulation to the skin after 24 hours, but Example 4 containing Mannula mushroom fruiting body extract and Example 5 containing did not cause any skin seizure even after 24 hours, 48 hours, 72 hours after patching.
From the above results, it could be confirmed that the skin irritation caused by the substrate (surfactant, fragrance, alcohol) that causes irritation when mixed with the strands of the fruiting body of the mandala mushroom and the mandrel mushroom mycelium was used in cosmetics.
Example 6 Preparation of a Facial Water Containing Fruit Extract
To 8 g of 95% ethanol, 0.05 g of polypyrrolidone, 0.1 g of oleyl alcohol, 0.2 g of polyoxyethylene monooleate, 0.2 g of fragrance, 0.1 g of paraoxybenzoic acid methyl ester, a small amount of antioxidant, and a small amount of pigment It was. 0.05 g of mandala mushroom fruiting body extract obtained in Example 1 and 5 g of glycerin were dissolved in 85.33 g of purified water, and the mixed solution was added thereto, followed by stirring to prepare a lotion containing the mandala fruiting fruit extract.

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Example  7: Preparation of the lotion containing the mandrel mycelium culture medium

To 8 g of 95% ethanol, 0.05 g of polypyrrolidone, 0.1 g of oleyl alcohol, 0.2 g of polyoxyethylene monooleate, 0.2 g of fragrance, 0.1 g of paraoxybenzoic acid methyl ester, a small amount of antioxidant, and a small amount of pigment It was. 0.05 g of mandala mycelium mycelium culture solution obtained in Example 3 and 5 g of glycerine were dissolved in 85.33 g of purified water, and the mixed solution was added thereto followed by stirring to prepare a lotion containing the mandarin mycelium mycelium culture solution.
Example 8 Latex Preparation Containing Pleurotus eryngii Fruit Extract
1.2 g of cetyl alcohol, 10 g of squalane, 2 g of vaseline, 0.2 g of paraoxybenzoic acid ethyl ester, 1 g of glycerin monoesteralide, 1 g of polyoxyethylene (20 molar addition) monooleate and 0.1 g of fragrance at 70 ° C The mixture was heated and dissolved, and 0.5 g of the mandala fruit fruit extract obtained in Example 1, 5 g of dipropylene glycol, 2 g of polyethylene glycol-1500, 0.2 g of triethanolamine and 76.2 g of purified water were dissolved at 75 ° C. Both were mixed and emulsified, and then cooled to prepare an oil-in-water (O / W) type emulsion.

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Example  9: Preparation of Latex Containing Mandala Mushroom Mycelia Culture Solution

1.2 g of cetyl alcohol, 10 g of squalane, 2 g of vaseline, 0.2 g of paraoxybenzoic acid ethyl ester, 1 g of glycerin monoesteralide, 1 g of polyoxyethylene (20 molar addition) monooleate and 0.1 g of fragrance at 70 ° C Heat-dissolve and melt | dissolve, 0.5 g of the mandala mycelium culture medium obtained in Example 3, 5 g of dipropylene glycol, 2 g of polyethyleneglycol-1500, 0.2 g of triethanolamine, and 76.2 g of purified water were melt | dissolved at 75 degreeC. Both were mixed and emulsified, and then cooled to prepare an oil-in-water (O / W) type emulsion.
Example 10 Preparation of Cosmetic Solution Containing Fruit Extract
To 5 g of 95% ethyl alcohol, 1.2 g of polyoxyethylene sorbitan monooleate, 0.3 g of kitulose, 0.2 g of sodium hyaluronate, 0.2 g of vitamin E-acetate, 0.2 g of sodium licorice, 0.1 g of paraoxybenzoic acid ethyl ester To prepare a cosmetic liquid by mixing 1 g of the fruiting body extract of Manduk mushroom obtained in Example 1 and a suitable amount of pigment.

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Example  11: containing mandrel mycelium culture medium Essence  Produce

To 5 g of 95% ethyl alcohol, 1.2 g of polyoxyethylene sorbitan monooleate, 0.3 g of kitulose, 0.2 g of sodium hyaluronate, 0.2 g of vitamin E-acetate, 0.2 g of sodium licorice, 0.1 g of paraoxybenzoic acid ethyl ester To prepare a cosmetic liquid by mixing 1 g of the Mycelial mycelia culture medium obtained in Example 3 and a suitable amount of pigment.

Claims (18)

A pharmaceutical composition for the prevention or treatment of atopic dermatitis, characterized by containing the extract of fruiting body ( Lyophyllum ulmarium ) fruiting body as an active ingredient.
Strand only mushrooms (Lyophyllum ulmarium) or mycelial extract only strands mushroom (Lyophyllum ulmarium) of atopy, characterized in that it contains a culture solution obtained from the culture of the mycelium, as an active ingredient for preventing or treating dermatitis pharmaceutical composition.
The method according to any one of claims 1 or 2,
The pharmaceutical composition for preventing or treating atopic dermatitis,
A pharmaceutical composition for preventing or treating atopic dermatitis, which comprises 0.001 to 90.0% by weight of the whole of the composition, the culture solution obtained from the cultivation of the mandala mushroom fruiting body extract or mandala mushroom mycelium extract or mandala mushroom mycelium.
delete delete delete For the improvement of atopic skin, characterized in that it contains any of the culture solution obtained from the culture of the fruiting body extract of Lyophyllum ulmarium or Lyophyllum ulmarium mycelium or Lyophyllum ulmarium mycelium as an active ingredient Cosmetic composition.
For stimulating skin irritation, it comprises any of the culture solution obtained from the culture of the fruiting body extract of Lyophyllum ulmarium or Lyophyllum ulmarium mycelium extract or Lyophyllum ulmarium mycelium as an active ingredient Cosmetic composition. delete delete delete delete delete delete delete delete delete delete