KR20040110441A - Cosmetic composition containing mixture extract of OUGIDAN(Paecilomyces japonica, Acanthopanax senticosus, Ganoderam Lucidum, Deer antlers and Ginseng), and the method of manufacturing thereof with supercritical fluids extraction - Google Patents

Abstract

PURPOSE: Provided is a cosmetic composition containing the extract of ougidan(5 kinds of natural medicinal herbs including Paecilomyces japonica, Acanthopanax senticosus, Ganoderam lucidum, deer antlers and ginseng), and method of manufacturing the same with supercritical fluids extraction. The composition has anti-inflammatory effect, skin wrinkle removing effect, moisturizing effect and skin elastic increasing effect to prevent skin aging. CONSTITUTION: The extract of ougidan(5 kinds of natural medicinal herbs including Paecilomyces japonica, Acanthopanax senticosus, Ganoderam lucidum, deer antlers and ginseng) is obtained by the steps of: mixing Paecilomyces japonica, Acanthopanax senticosus, Ganoderam lucidum, deer antlers and ginseng; mixing the extraction solvent with the mixture, followed by stirring; supplying supercritical fluids to the mixture in the extractor; increasing inner temperature of the extractor to 40 deg.C with a speed of 0.5 deg.C /min maintaining the pressure of the extractor to 300 atm; and maintaining the temperature and pressure of the extractor to 30-50 deg.C and 200-350 atm, respectively for 50 minutes, then reducing the pressure to give the extract of ougidan.

Description

Cosmetic composition containing a mixed extract of eogidan (Central cordwort, prickly pear, ganoderma lucidum, antler and ginseng) using a supercritical fluid extraction technique and a method for preparing the same Lucidum, Deer antlers and Ginseng), and the method of manufacturing Julia with supercritical fluids extraction}

The present invention is a natural herbal five natural products, that is, Paceilomyces japonica , Acanthopanax senticosus, Ganoderma lucidum Karst. , Cervus elaphus L. and Panax ginseng CA Meyer The present invention relates to a cosmetic composition having a combination of anti-inflammatory effect, wrinkle reduction, elasticity enhancement and recovery of moisturizing ability by obtaining a mixed extract through a critical fluid extraction technique and applying it to a cosmetic, and having an effect of preventing skin aging, and a preparation method thereof.

Cordyceps sinensis is a type of asymptomatic fungus in which fruiting bodies (mushrooms) are formed. When analyzing the components of Cordyceps sinensis, 11 kinds of amino acids necessary for the human body and 10 kinds of amino acids with medicinal efficacy account for more than 60% of the total content, respectively, and cordycepic, an isomer of quinic acid. acid) about 7%. In addition, linolenic acid, an antioxidant lipid required for cell activation, and 3'-deoxyadenosine, a nucleic acid, are included. In addition, various pharmacological actions are known, such as immune enhancement (see Cunningham, KG, W. Manson. FS Spring and SA Hutchinson. 1950. Cordycepin, a metabolic product from cultures of (Linn.), Link. Nature, 166, 949). .

Prickly Ogalpi is a deciduous shrub belonging to the Ogalpi family and has many uses of its bark as a medicinal herb. It has excellent pharmacological effects such as anti-aging, immune function improvement, chronic fatigue recovery, skin care, inflammatory disappearance, anti-stress, and acidification of cells (Kashigapipi cultivation association data, native herbal medicine method, Taeil Publishing Co., Jin-Kyu Choi).

Ganoderma lucidum promotes blood circulation and makes skin healthy. It has anti-inflammatory and anti-cancer effects to protect skin cells and has anti-allergic effect.

Deer antler gives skin nourishment and fatigue by promoting metabolism, and gives anti-aging effect by preventing skin dryness and wrinkles by supplying protein. It helps keep your skin healthy.

Ginseng is effective for the treatment and prevention of skin wounds and troubles by activating skin cells, and smoothes the skin surface by promoting blood circulation and cleansing, and can inhibit skin aging with anti-oxidant effect along with antibacterial, anti-cancer and anti-inflammatory effects. Known.

The prior art extracting these five active ingredients is as follows.

After the mixed extract is added to a solvent such as butylene glycol or propylene glycol, extraction is performed by heating at 50 to 95 ° C. for 3 to 20 hours in a distillation apparatus equipped with a cooling condenser to prevent the solvent from evaporating, or 5 to 37 ° C. The active ingredient was extracted by immersing in 1-15 days.

In addition, since the same effect is obtained even when each of the natural product extract and then mixed with each other, this method is equally applicable. Using the distillation apparatus equipped with a cooling condenser while recovering the solvent evaporated, the five natural product mixture extracts are concentrated under reduced pressure to obtain an extract.

However, since the general solvent extraction method is heated to a high temperature, there is a problem that the natural active component is destroyed by evaporation, pyrolysis and oxidation.

Therefore, the present inventors confirmed that the Ogidan extract, which is extracted from five herbal substances that have been proven through a long clinical experience, by supercritical fluid extraction, has an excellent anti-inflammatory and moisturizing effect, as well as cytoprotective and proliferative effects, compared to general solvent extracts. The present invention has been found to contribute more effectively to the prevention of wrinkles, reinforcement and recovery of moisturizing ability when introduced into cosmetics.

Therefore, it is an object of the present invention to provide an Ogidan extract prepared by extracting Cordyceps sinensis, Prickly Pear, Ganoderma lucidum, Deer Antler and Ginseng by supercritical fluid extraction.

In addition, it is an object of the present invention, by containing the Ogidan extract, the cell protection from the ultraviolet rays, the cell proliferation ability and excellent anti-inflammatory and moisturizing ability of the natural products can be more than when extracted in the general solvent extraction method, each active ingredient The synergistic effect of the skin to effectively reduce wrinkles, enhance elasticity and moisturizing ability to effectively contribute, and to provide a cosmetic composition without problems in terms of safety and stability.

Figure 1 is a graph showing the elasticity enhancing effect of the Ogidan extract of the present invention.

Figure 2 is a graph showing the effect of increasing the moisturizing power of the Ogidan extract of the present invention.

Figure 3 is a graph showing the anti-inflammatory effect of the Ogidan extract of the present invention.

The present inventors have studied with regard to natural herbal extracts of natural herbal ingredients to overcome the aging of human skin, wrinkles, loss of elasticity and moisturizing ingredients. In other words, five natural herbal substances, Ogidan, embodied in the concept of Heaven, Earth, Phosphorus, Phosphorus, Mountain, and Water, strengthen the tonic, vitality, and metabolism. Since it is known to have been known, it is thought to be associated with skin aging.

Accordingly, the present inventors named the five herbal substances Cordyceps sinensis, Prickly Pear, Ganoderma lucidum, antler and ginseng as "Ogidan", and the extract obtained by extracting such Ogidan by supercritical fluid extraction is called "Ogidan Extract". Named it.

Therefore, the present invention relates to an oogidan extract prepared by extracting Cordyceps sinensis, Prickly Pear, Ganoderma lucidum, Deer Antler and Ginseng by supercritical fluid extraction.

Supercritical fluids are defined as "fluids above the critical temperature and pressure," and have unique characteristics that distinguish them from conventional solvent extraction methods. Substances in the supercritical state change pressures near their critical point, and many properties such as density, viscosity, diffusion coefficient, and polarity are continuously changed very largely from gas to liquid. When the carbon dioxide of the liquid is put into a closed container and heated, the boundary of the gas-liquid interface gradually disappears as the temperature and pressure increase. Supercritical fluid is a heavy fluid that fills a closed space like a gas. Supercritical fluid technology takes advantage of supercritical fluids such as high dissolving power, fast mass transfer and heat transfer, low viscosity, high diffusion coefficient and fast penetration into micropores due to low surface tension.

The supercritical fluid extraction method according to the present invention uses carbon dioxide as the supercritical fluid and the extraction process is as follows:

1) mixing Cordyceps sinensis, Prickly Pear, Ganoderma lucidum, Deer Antler and Ginseng, pulverizing and mixing with a solvent to stir;

2) feeding the mixture into a supercritical extraction tank and supplying a supercritical fluid;

3) maintaining the pressure of the extraction tank at 300 atm and raising the temperature inside the extraction tank to 40 ° C. at a rate of 0.5 ° C./min; And

4) maintaining the temperature and pressure of the extraction tank for 30 minutes at 30 ~ 50 ℃, 200 ~ 350 atm and then reducing the pressure to atmospheric pressure to obtain the Ogidan extract.

Through the extraction in the supercritical conditions as described above, as well as the effective obtaining of the active ingredient, each effective ingredient is well expressed after each extraction and the synergistic effect of each other can be obtained.

Looking at the manufacturing method of the Ogidan extract, which is a mixed extract of Cordyceps sinensis, Prickly Pear, Ganoderma lucidum, Deer Antler and Ginseng according to the present invention in more detail.

First, Cordyceps sinensis, Prickly Pear, Ganoderma lucidum, Deer Antler and Ginseng are mixed and ground to mix butylene glycol and ethanol, each of which is mixed with 62.7% by weight of crushed natural products, 33.9% by weight of butylene glycol and 3.4% by weight of ethanol. After stirring and left for 1 hour.

The mixture is introduced into a supercritical extraction tank and the pressure is increased by supplying carbon dioxide to the extraction tank using a high pressure gas pump. When the pressure of the extraction tank reaches 300 atm, the supply of carbon dioxide is stopped. At this time, the temperature inside the extraction tank is similar to the room temperature, and the reason is connected to the pressure pump and the extraction tank by a long pipe (1m) close to the pressure pump is heated, but the temperature inside the extraction tank is actually unchanged.

The temperature inside the extraction tank is heated up to 40 ° C. at a rate of 0.5 ° C./min using a heating wire surrounding the outside of the extraction tank using a temperature controller. The pressure of the extraction tank due to the elevated temperature discharges a part of carbon dioxide by using a pressure regulator attached to the outlet of the extraction tank and maintains the pressure at 300 atm.

The temperature and pressure of the extraction tank are maintained at 40 ° C. and 300 atm for 50 minutes. The high pressure gas pump is restarted and carbon dioxide is continuously supplied to the extraction tank at a flow rate of 20 L / min. At the same time, the extract is discharged using a pressure regulator attached to the outlet and the temperature and pressure of the extraction tank are kept constant. Emissions released through the pressure regulator transfer carbon dioxide to solid dry ice due to pressure and temperature drop. This may cause clogging of the tube at the final outlet. To prevent this, maintain the temperature of the pressure regulator to 70 ℃.

The released extract is decompressed to atmospheric pressure to release carbon dioxide into the atmosphere, and the extract of the active ingredient and butylene glycol are collected together in the collector. The above process is carried out for 12 hours to obtain an extract, and then the extraction operation is terminated.

The preferred dry weight ratio of each of the five extracts to the Ogidan extract is 5-10% by weight of Cordyceps sinensis, 10-20% by weight of Prickly Pear, 5-10% by weight of Ganoderma lucidum, 2-5% by weight of antler and 20-30% by weight of ginseng. It is good to make it%.

In addition, the present invention relates to a cosmetic composition containing an extract of Ogidan by the supercritical fluid extraction method, and by introducing the Ogidan extract into the skin, they have anti-inflammatory and moisturizing effects along with cell protection, cell proliferation and UV protection. It relates to a cosmetic composition superior to the extract obtained through the general solvent extraction method.

The cosmetic composition according to the present invention preferably contains the Ogidan extract in an amount of 0.1 to 10% by weight, preferably 0.5 to 7% by weight, based on the total composition (drying conditions: sample 1g, 105 ° C, 2 time).

If the content of the Ogidan extract in the cosmetic composition is less than 0.1 dry weight%, it is not preferable to show the effectiveness, if more than 10 dry weight% problem in formulating, the optimum concentration in consideration of efficacy and stability is 0.5-7 dry weight %to be.

Hereinafter, the configuration and effect of the present invention through the examples and test examples in more detail. However, these examples are intended to illustrate the present invention, it is obvious to those skilled in the art that the scope of the present invention is not limited to these examples.

Example 1 Preparation of Ogidan Extract

50 g of Cordyceps sinensis, 100 g of prickly pear, 100 g of Ganoderma lucidum mushroom, 20 g of antler, and ginseng 150 g were mixed and mixed with 200 g of butylene glycol and 20 g of ethanol, followed by stirring for 1 hour.

The mixture was introduced into a supercritical extraction tank and then the carbon dioxide was supplied to the extraction tank using a high pressure gas pump to stop the supply of carbon dioxide when the pressure reached 300 atm. The temperature inside the extraction tank was heated to 40 ° C. at a rate of 0.5 ° C./min through a heating wire surrounding the outside of the extraction tank using a temperature controller. The temperature and pressure of the extraction tank were maintained at 40 ° C. and 300 atmospheres for 50 minutes while partially discharging carbon dioxide using a pressure regulator attached to the outlet of the extraction tank. Then, the high-pressure gas pump was restarted to continuously supply carbon dioxide to the extraction tank at a flow rate of 20 L / min. At the same time, the extract was discharged using a pressure regulator attached to the outlet, and the temperature and pressure of the extraction tank were kept constant at 40 ° C and 300 atm. While maintaining the temperature of the pressure regulator at 70 ° C., the emission released through the pressure regulator transferred carbon dioxide to solid dry ice due to pressure and temperature drop. The released extract was decompressed to atmospheric pressure to release carbon dioxide into the atmosphere, and the extract of the active ingredient and butylene glycol of the Ogidan were recovered together into the collector. The above procedure was carried out for 12 hours to obtain an extract, and the extraction operation was terminated to obtain 29.5 g (dry weight) of the natural extract.

Example 2 Preparation of Ogidan Extract

26 g (dry weight) of the natural product extract was obtained using the same method as in Example 1, except that only butylene glycol was used alone without using butylene glycol and ethanol as a solvent.

Example 3 Preparation of Ogidan Extract

Except for using butylene glycol and water in place of butylene glycol and ethanol in Example 1, using the same method as in Example 1, 27g (dry weight) of the natural product extract was obtained.

Example 4 Preparation of Ogidan Extract

Except for using ethanol and water instead of butylene glycol and ethanol as a solvent in Example 1, 25g (dry weight) of the natural product extract was obtained in the same manner as in Example 1.

Example 5 Preparation of Ogidan Extract

23 g (dry weight) of a natural product extract was obtained using the same method as Example 1 except for using ethanol alone instead of butylene glycol and ethanol used as a solvent in Example 1.

Example 6 Preparation of Ogidan Extract

24 g (dry weight) of the natural product extract was obtained using the same method as in Example 1 except that only water was used instead of butylene glycol and ethanol as the solvent.

Comparative Example 1

50 g of Cordyceps sinensis, 100 g of prickly pear, 100 g of ganoderma lucidum, 50 g of antler, 20 g of ginseng, and 150 g of crushed mixture were mixed with 3.7 kg of butylene glycol and 300 g of ethanol, stirred for 10 hours and then in a distillation apparatus equipped with a cooling condenser. It heated at 5 degreeC for 5 hours, and obtained 6.3 g (dry weight) of an extract.

Comparative Example 2

7.4 g (dry weight) of a natural product extract was obtained using the same method as in Comparative Example 1, except that only butylene glycol was used alone without using butylene glycol and ethanol as a solvent in Comparative Example 1.

Comparative Example 3

Except for using butylene glycol and water instead of butylene glycol and ethanol as a solvent in Comparative Example 1 using the same method as in Comparative Example 1, 7.0g (dry weight) of the natural product extract was obtained.

[Comparative Example 4]

5.5 g (dry weight) of the natural product extract was obtained using the same method as in Comparative Example 1, except that ethanol and water were used instead of butylene glycol and ethanol as the solvent in Comparative Example 1.

[Comparative Example 5]

Instead of butylene glycol and ethanol used as the solvent in Comparative Example 1, using ethanol alone and using the same method as in Comparative Example 1 except that the temperature of the heating conditions to 60 ℃, 4.0g (dry weight) of natural product extract Got.

Comparative Example 6

6.0 g (dry weight) of the natural product extract was obtained using the same method as in Comparative Example 1, except that only water was used instead of butylene glycol and ethanol as the solvent in Comparative Example 1.

Test Example 1 Cell Protection Effect Test from Ultraviolet Rays

1) test method

Human fibroblasts were placed in well plates at 2 × 10 ⁵ cells / well and 0.2 ml of DMEM medium containing 10% FBS was supplied, followed by incubation in a CO ₂ incubator for 24 hours. The Ogidan extracts of Examples 1 to 6 and the extracts of Comparative Examples 1 to 6 were dissolved in DMSO, respectively, and then irradiated with ultraviolet rays of 2MED for 5 minutes, which were incubated for 24 hours. MTT assay was performed to determine the viability of the cells. In the MTT assay, 0.1 ml of MTT solution was added to the cultured cells, followed by 4 hours of incubation. The medium was removed, and 0.5 ml of DMSO was added to formazan. It was. In addition, absorbance was measured as a control, which was tested with DMSO, a solvent in which the solid content of each example was dissolved, and the cell protective effect was determined from the difference in relative absorbance with the control.

2) Test result

According to the above method, the effect of protecting the cells from ultraviolet rays of the extracts extracted in Examples 1 to 6 and Comparative Examples 1 to 6 was measured, and the results are shown in Table 1 below. From the above results, it was found that the Ogidan extracts of Examples 1 to 6 according to the present invention had a significantly superior cell protective effect compared to the extracts of Comparative Examples 1 to 6 prepared by the conventional extraction method.

Concentration of the sample contained in the reaction solution (µg / ml) Cell protective effect (%) Example Comparative example One 100 92 (0.95999) 86 (0.9003) 2 100 87 (0.91038) 79 (0.82531) 3 100 87 (0.9053) 77 (0.80121) 4 100 80 (0.83739) 68 (0.70612) 5 100 49 (0.51261) 46 (0.47865) 6 100 79 (0.81983) 62 (0.64894) Cytoprotective effect (%) = absorbance of the test group / absorbance of the control group × the number in the parenthesis is the absorbance (nm). For reference, the absorbance of the control group is 1.0449 nm

[Test Example 2] Cell protective effect test from Superoxide radical

1) test method

Human fibroblasts were placed in well plates with 2 × 10 ⁵ cells / well and 0.2 ml of DMEM medium containing 10% FBS was supplied, followed by incubation in a CO ₂ incubator for 24 hours. The Ogidan extract of Examples 1-6 and the extracts of Comparative Examples 1-6, which were dissolved in DMSO, were treated and incubated for 30 minutes. 10 μl of 1.5 mM xanthine and 10 μl of 0.25 U xanthin oxidase were added to the cultured cells to generate superoxide radicals, and then cultured in a CO ₂ incubator for 24 hours. MTT assay was performed to determine the viability of the cells. In the MTT assay, 0.1 ml of MTT solution was added to the cultured cells, followed by 4 hours of incubation. The medium was removed, and 0.5 ml of DMSO was added thereto. The absorbance was measured at 570 nm using an ELISA reader. In addition, absorbance was measured as a control, which was tested with DMSO, a solvent in which the solid content of each example was dissolved, and the cell protective effect was determined from the difference in relative absorbance with the control.

2) Test result

According to the above method, the cell protective effect from the superoxide radicals of the extracts extracted in Examples 1 to 6 and Comparative Examples 1 to 6 were measured, and the results are shown in Table 2. From the above results, it can be seen that the Ogidan extracts of Examples 1 to 6 according to the present invention have a remarkably superior protective effect from superoxide radicals compared to the extracts of Comparative Examples 1 to 6 prepared by the conventional extraction method.

Concentration of the sample contained in the reaction solution (µg / ml) Cell protective effect (%) Example Comparative example One 100 92 (0.95989) 82 (0.85875) 2 100 72 (0.75268) 68 (0.70725) 3 100 85 (0.88816) 78 (0.81457) 4 100 82 (0.85437) 76 (0.79412) 5 100 63 (0.65829) 60 (0.62794) 6 100 79 (0.82547) 66 (0.68963) Cytoprotective effect (%) = absorbance of the test group / absorbance of the control group × the number in the parenthesis is the absorbance (nm). For reference, the absorbance of the control group is 1.0449 nm

Test Example 3 Cell Proliferation Effect Test

1) test method

100 μl of a 5% fetal bovine serum (FBS) solution was added to a 96 μ-well plate, and then the Ogidan extract of Examples 1 to 6 and Comparative Examples 1 to 6 in DMEM medium containing 2% FBS. The extracts were each diluted to a certain concentration, diluted, and added again to 100 μl. Fibroblasts obtained from the foreskin tissue of newborns were added to a 4000 μl per well in 100 μl medium using a hemocytometer, and then cultured for 7 days. After the incubation, the supernatant was removed and washed again by adding 200 μl of 5% phosphate buffered saline (PBS), and then 150 μl of DMSO was added per well and shaken for 10 minutes to measure absorbance at 570 nm. In addition, absorbance was measured as a control, which was tested with DMSO, a solvent in which the solid content of each example was dissolved, and the cell proliferation effect was determined from the difference in relative absorbance with the control.

2) Test result

According to the above method, the cell proliferation effect of the extract extracted in Examples 1-6 and Comparative Examples 1-6 was measured, and the results are shown in Table 3. From the above results, it was found that the Ogidan extracts of Examples 1 to 6 according to the present invention had an excellent cell proliferation effect compared to the extracts of Comparative Examples 1 to 6 prepared by the conventional extraction method.

Concentration of the sample contained in the reaction solution (µg / ml) Cell proliferation effect (%) Example Comparative example One 100 130 (1.59880) 113 (1.62345) 2 100 127 (1.55119) 116 (1.41321) 3 100 110 (1.34112) 106 (1.29875) 4 100 104 (1.26811) 99 (1.21002) 5 100 97 (1.18735) 90 (1.09815) 6 100 113 (1.38127) 104 (1.27026) Cell proliferation effect (%) = absorbance of test group / absorbance of control group × 100 Figure in parentheses is absorbance (nm).

Test Example 4 Moisturizing Effect Test

1) test method

5g each of Ogidan extract of Comparative Example 1 and extract of Comparative Example 1 were placed in a dish, which was left under conditions of 41% relative humidity in a desiccator containing CaCl ₂ and a temperature of 25 ° C. The change in weight after a certain period of time was measured over time.

2) Test result

According to the above method, the moisturizing effect of the extract extracted in Examples 1 to 6 and Comparative Example 1 was measured, and the results are shown in Table 4. From the above results, it was found that the extracts of Ogidan of Examples 1 to 6 according to the present invention were superior to the extract of Comparative Example 1 prepared by the conventional extraction method.

Measurement time (hr) Moisture Content (%) Example 1 120 53.6 Example 2 120 47.1 Example 3 120 51.3 Example 4 120 47.7 Example 5 120 45.8 Example 6 120 49.4 Comparative Example 1 120 43.3 Moisture Content (%) = Residual Sample Volume (g) / Initial Sample Volume (g) × 100

Test Example 5 Safety Test of Ogidan Extract

In order to test the safety of the Ogidan extract, the primary skin irritation test, the primary ocular mucosal irritation test and the skin sensitization test were performed on the rats using the Ogidan extract of Example 1, and the results are shown in the following table. 5-7 (test period: April 4, 2003-May 20, 2003). Table 5 below shows the results of the primary skin irritation test.

time Hong Van Edema 30-60 minutes 0 0 24 hours 0 0 48 hours 0 0 72 hours 0 0 * Experimental animal species: Albino Rabbit from New Zealand, age and weight range at the start of administration: 10 weeks, 2.5㎏ ± 20g

From the above results, it was confirmed that the Ogidan extract of the present invention is a safe substance without irritation such as skin erythema or edema.

In addition, Table 6 below shows the results of the primary eye mucosal irritation test.

opinion 1 hours 24 hours 48 hours 72 hours Corneal haze 0 0 0 0 Over iris 0 0 0 0 Corneal Redness Edema 0 0 0 0 * Experimental animal species: Albino Rabbit from New Zealand, age and weight range at the start of administration: 10 weeks, 2.5㎏ ± 20g

From the above results, it was confirmed that the Ogidan extract of the present invention is a safe substance without eye mucosal irritation such as corneal haze, iris abnormalities or corneal red edema.

In addition, Table 7 below shows the results of the primary skin sensitization test.

time Reduction rate (%) Rating Sensitization degree 24 hours 6 I Very weak 48 hours 3 I Very weak * Experimental animal species: Hartly male Guinea-Pig, * Age and weight range at the start of administration: 10 weeks, 300-500 g

In the above results, the sensitization rate is calculated in the usual manner from the number of animals that showed a positive response to the test and their skin reactions scored, and when the sensitization rate is 0 to 8%, grade I Note that it is common to set the degree of sensitivity to very weak. Therefore, it was confirmed that the Ogidan extract of the present invention is a safe substance in skin sensitization.

In addition, in the human skin patch test, all reactions were negative for 30 minutes and 24 hours after sample removal, and no abnormality was confirmed even on day 7 after sample removal.

That is, the Ogidan extract was proved to be a very safe substance from the above safety test results.

[Test Example 6] Measurement of the stability of physical properties of Ogidan extract

Discoloration measurement generally used for measuring physical properties of cosmetic components at room temperature, 40 ° C. and daylight, in order to evaluate how stable the physical properties of the Ogidan extract of Example 1 can be used with cosmetics. Was carried out, and the results are shown in Table 8 below.

Date condition One 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Room temperature - - - - - - - - - - - - - - - 40 ℃ - - - - - - - - - - - - - - - daylight - - - - - - - - - - - - - - - Date condition 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 Room temperature - - - - - - - - - - - - - - - 40 ℃ - - - - - - - - - - - - - - - daylight - - - - - - - - - - - + + + + -; No color change, +; Start discoloration, ++; Changing a lot, +++; Complete discoloration

From the above results, the Ogidan extract of the present invention was found to be very stable at room temperature and 40 ° C, and almost negligible discoloration in daylight, but it was found to be a very stable substance without any further discoloration progression.

Test Example 7 Test for Increased Elasticity of Human Skin

The nutritional cream of Comparative Example 7 containing the nutrient cream of Formulation Example 1 and the general solvent extract prepared by containing the Ogidan extract of Example 1 was prepared, and the effect on the elasticity of the skin when the skin was applied was measured. In the test, 20 women aged 20-55 years had normal, oily, dry, and complex skin. After application twice a day to use in the evening, after 1 week, 2 weeks, 3 weeks and 4 weeks was carried out by measuring the elasticity of the skin using a skin elasticity meter (Ballisto meter). The results are shown in Table 9 and FIG. 1.

Formulation Example 1 and Comparative Example 7 Nutrition Cream

Raw material Formulation Example 1 Comparative Example 7 Ogidan Extract of Example 1 7.0 - Extract of Comparative Example 2 - 7.0 β-sitosterol 3.5 3.5 12-20 Acid Fiji-8 Estelle 3.0 3.0 Ceramide 0.7 0.7 Setes-4 2.0 2.0 cholesterol 3.5 3.5 Diecety phosphate 0.4 0.4 glycerin 5.0 5.0 Sunflower Seed Oil 22.0 22.0 Beeswax 1.0 1.0 Carbomer 0.5 0.5 Triethanolamine 0.5 0.5 antiseptic a very small amount a very small amount Spices a very small amount a very small amount Purified water Remaining amount Remaining amount

Formulation Example 1 Comparative Example 7 T ₀ T ₁ T ₂ T ₃ T ₄ T ₀ T ₁ T ₂ T ₃ T ₄ Elasticity Spring water 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 amplitude first 1.7 4.1 5.2 6.4 7.2 1.6 3.6 4.4 4.6 5.4 second 1.2 3.4 3.8 5.2 6.0 1.3 1.9 2.3 3.0 3.4 third 0.0 2.2 2.8 3.7 4.5 0.0 0.0 2.0 2.3 2.4 fourth 0.0 0.0 1.3 2.9 3.3 0.0 0.0 0.2 0.3 0.9 Average 0.725 2.425 3.275 4.55 5.25 0.725 1.375 2.225 2.55 3.025 (Note) T ₀ ; Before use, T ₁ ; After 1 week, T ₂ ; After 2 weeks, T ₃ ; After 3 weeks, T ₄ ; After 4 weeks

As can be seen from the above results, the nutrition cream of Formulation Example 1 containing the Ogidan extract according to the present invention was found to have an excellent skin elasticity effect by improving the skin condition compared to the nutrition cream of Comparative Example 7.

Test Example 8 Anti-inflammatory Test

After the left ear was used as the control site and the right ear as the test site, the ear was washed with ethanol before application of the extraction mixture, and 20 μl of the sample was continuously applied once a day for 4 days, and then left 1 hour after the last application. In the ear, ethanol was applied to the right ear and arachidonic acid (2 mg / ear) was applied. After 1 hour, the degree of ear edema was measured by using a micrometer on both ears three times.

As a sample used in the above process, indomethacin, arachidonic acid and non-steroidal anti-inflammatory analgesics were used and the Ogidan extracts of Examples 1 to 6 were used.

The anti-inflammatory effect was determined by calculating the degree of edema inhibition through Equation 1 below based on the arachidonic acid treatment group, and the results are shown in Table 10.

% Inhibition = (A-B) / A × 100

A: Average thickness of control ears (thickness of arachidonic acid treated ears-thickness of untreated ears)

B: thickness of the ears of the extraction mixture application group (thickness of the sample-treated ear-thickness)

Figure 3 is a graph showing the anti-inflammatory effect of the Ogidan extract of Example 1.

Anti-inflammatory effect sample Final concentration in the reaction solution (㎍ / ml) menstruum Ear thickness (㎛) % Inhibition Before sample processing After sample processing Indomethacin 1.0 ethanol 321 466 43.4 Arachidonic acid 2mg / ear ethanol 298 554 - Example 1 1.0 ethanol 321 516 23.8 Example 2 1.0 ethanol 241 446 19.9 Example 3 1.0 ethanol 286 490 20.3 Example 4 1.0 ethanol 284 496 17.2 Example 5 1.0 ethanol 259 467 18.8 Example 6 1.0 ethanol 284 503 14.5

As can be seen in Table 10, the Ogidan extract of Examples 1 to 6 according to the present invention was confirmed to have an anti-inflammatory effect.

[Test Example 9] Wrinkle improvement effect test

For the nutrient essence of Comparative Example 8 containing the nutrient extract of Formulation Example 2 and the general solvent extract formulated containing the Ogidan extract of Example 1, to determine the wrinkle improvement effect according to skin elasticity enhancement Formulation Example 2 was applied twice a day to the left eye edge, and Comparative Example 8 was applied to the right eye edge. After 45 days after each treatment, the elasticity of the skin surface was measured by Cutometer SEM 474. Skin elasticity measurement by the cutometer SEM 474 is to measure the depth of wrinkles, the smaller the value is the elasticity is good and the wrinkles are relaxed. The elasticity value was expressed as a percentage decrease compared to the measured value before treatment, and the average value of 20 subjects was obtained and shown in Table 11.

Formulation Example 2 and Comparative Example 8 Nutritional Essence

Raw material Formulation Example 2 Comparative Example 8 Ogidan Extract of Example 1 7.0 - Extract of Comparative Example 2 - 7.0 β-cytosterol 1.50 1.50 12-20 acid pig-8 ester 2.00 2.00 Ceramide 0.7 0.7 Setes-4 1.2 1.2 cholesterol 1.5 1.5 Diecety phosphate 0.4 0.4 glycerin 5.0 5.0 Sunflower Seed Oil 5.0 5.0 Cyclomethicone 2.0 2.0 Carbomer 0.2 0.2 Xanthan Gum 0.2 0.2 antiseptic a very small amount a very small amount Spices a very small amount a very small amount Purified water Remaining amount Remaining amount

sample Skin elasticity (wrinkle depth reduction) effect (%) 30 days 45 days Formulation Example 2 18.1 29.5 Comparative Example 8 11.2 21.4

As can be seen in Table 11, it was found that Formulation Example 2, which is a cosmetic composition containing the Ogidan extract according to the present invention, has an effect of improving wrinkles by increasing skin elasticity as compared to Comparative Example 8.

Test Example 10 Moisturizing Effect Test

For the nutrition lotion of Comparative Example 9 containing the nutrient lotion of Formulation 3 and the general solvent extract, which was formulated with the Ogidan extract of Example 1, was formulated inside the left arm of 20 people to test the skin moisturizing effect. Example 3 and Comparative Example 9 were applied. After each application, the moisture on the skin surface was measured over time by Corneometer CM 825. Skin moisture measurement by Corneometer CM 825 is a measure of the moisture content of the skin, the higher the value, the better the moisture. The average value of 20 subjects was obtained and shown in Table 12 and FIG. 2. The test was carried out at a temperature of 20 ° C. and a relative humidity of 20%.

Formulation Example 3 and Comparative Example 9 Nutritional Lotion

Raw material Formulation Example 3 Comparative Example 9 Ogidan Extract of Example 1 7.0 - Extract of Comparative Example 2 - 7.0 β-sitosterol 1.70 1.70 Polysorbate 60 1.00 1.00 Ceramide 0.7 0.7 Setes-4 1.2 1.2 cholesterol 1.5 1.5 Diecety phosphate 0.4 0.4 glycerin 5.0 5.0 Sunflower Seed Oil 8.0 8.0 Cyclomethicone 1.0 1.0 Carbomer 0.2 0.2 Xanthan Gum 0.3 0.3 Triethanolamine 0.2 0.2 antiseptic a very small amount a very small amount Spices a very small amount a very small amount Purified water Remaining amount Remaining amount

Time (min) 5 10 15 20 25 30 Formulation Example 3 19.4 7.99 5.92 5.33 5.05 4.76 Comparative Example 9 20.4 7.60 4.24 3.68 3.32 3.08

As can be seen from Table 12, it can be seen that the formulation example 3, which is a cosmetic containing the extract of Ogidan according to the present invention, maintains the moisture content of the skin to some extent even when compared to Comparative Example 9.

Ogidan extract according to the present invention can be formulated in the following Formulation Example 4 and Formulation Example 5 in addition to Formulation Examples 1 to 3, the present invention is not limited to these formulations.

Formulation Example 4 Lotion (Skin Lotion)

Raw material Content (% by weight) Ogidan Extract of Example 1 7.0 glycerin 2.0 Butylene Glycol 1.0 Propylene glycol 2.0 Fiji-26-Bootes-26 / Pig-40 Hydrogenated Castor Oil 1.0 ethanol 10.0 Triethanolamine 0.1 antiseptic a very small amount Pigment a very small amount Spices a very small amount Purified water Remaining amount

Formulation 5 Massage Cream

Raw material Content (% by weight) Ogidan Extract of Example 1 7.0 Beeswax 5.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.8 Lipophilic glyceryl stearate 0.8 Liquid paraffin 30.0 Squalane 5.0 Caprylic / Capric Triglycerides 4.0 Carbomer 0.3 glycerin 5.0 Butylene Glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.3 antiseptic a very small amount Pigment a very small amount Spices a very small amount Purified water Remaining amount

Supercritical fluid extraction according to the present invention can be extracted with almost no destruction of the naturally active ingredients. In addition, the content of the extract of the natural active ingredient is a small amount of the general solvent extract, but the supercritical fluid extraction method was able to obtain a yield of 3 to 4 times or more. Therefore, when applying the same amount of extract may include a larger amount of the natural active ingredient than the general solvent extract, it was possible to obtain a natural ingredient that is not destroyed the applied natural active ingredient. In addition, the cost of extraction is also very economical.

In the present invention, by extracting Cordyceps sinensis, Prickly Pear, Ganoderma lucidum, Deer Antler and Ginseng through supercritical fluid extraction method and introducing it into the cosmetics, cell extracting ability, cell proliferation and excellent moisturizing ability of the extract from the general solvent extract Through this, it was possible to effectively provide effects such as skin anti-aging, which acts in combination with skin wrinkle reduction, elasticity enhancement and moisturizing recovery.

Claims (9)

1) mixing Cordyceps sinensis, Prickly Pear, Ganoderma lucidum, Deer antler and Ginseng as eogidan components, pulverizing, mixing with a solvent and stirring; 2) feeding the mixture into a supercritical extraction tank and supplying a supercritical fluid; 3) maintaining the pressure of the extraction tank at 300 atm and raising the temperature inside the extraction tank to 40 ° C. at a rate of 0.5 ° C./min; And 4) maintaining the temperature and pressure of the extraction tank at 30 to 50 ° C., 200 to 350 atm for 50 minutes, and then decompressing to atmospheric pressure to obtain an ogidan extract; Ogi stage extraction method, characterized in that extracted with a supercritical fluid extraction method comprising a. According to claim 1, wherein the natural products are contained in a dry weight ratio of 5 to 10% by weight of Cordyceps sinensis, 10 to 20% by weight of prickly vinegar, 5 to 10% by weight of Ganoderma lucidum, 2 to 5% by weight of antler and 20 to 30% by weight of ginseng Ogidan extraction method characterized in that. The method according to claim 1, wherein the solvent is at least one selected from the group consisting of butylene glycol, water and ethanol. The method according to claim 1, wherein carbon dioxide is used as the supercritical fluid. Ogidan extract prepared by the extraction method according to claim 1. Cosmetic composition, characterized in that it contains the Ogidan extract according to claim 5. 7. The cosmetic composition according to claim 6, wherein the cosmetic composition contains 0.1 to 10% by weight of the total weight of the total composition. 7. The cosmetic composition according to claim 6, wherein the cosmetic composition is formulated as a lotion, skin lotion, nutrition lotion, nutrition cream, massage cream or nutrition essence. The cosmetic composition according to claim 6, wherein the extract is contained for protecting the cell from ultraviolet rays, for proliferating the cell or for moisturizing it.