KR20020093251A - A cosmetic composition containing mixing extract of shipjangsaeng-related natural substance(Pinus sylvestris, Ganoderma lucidum Karst., Arbutin, Cervus elaphus L., Amyda sinensis, Germanum, Apis mellifera L., Panax ginseng C. A. Meyer, salmon egg, Citrus Medica Limonum) - Google Patents

Abstract

PURPOSE: A cosmetic composition containing an extract of 10 kinds of natural materials associated with ten longevity symbols which means long life and eternal life and contains pine tree, herb of eternal youth, crane, tortoise, rock, water, mountain, sun and cloud is provided. Therefore, the composition reduces skin wrinkle, reinforces skin elasticity and recovers moisturizing capability to prevent skin aging. CONSTITUTION: This cosmetic composition contains an extract of 5 to 15% by weight of Pinus sylvestris, 10 to 15% by weight of Ganoderma lucidum Karst., 1 to 5% by weight of arbutin, 15 to 25% by weight of Cervus elaphus L., 15 to 25% by weight of Amydae sinensis, 0.0001 to 0.0003% by weight of germanium, 10 to 15% by weight of Apis mellifera L., 5 to 15% by weight of Panax ginseng C. C. Meyer, 3 to 10% by weight of salmon roe and 10 to 15% by weight of Citrus Medica Limonum. The extract is obtained by soaking natural materials in a solvent at 5 to 37deg.C for 1 to 15 days or heating in a solvent at 50 to 95deg.C for 3 to 20hr.

Description

Cosmetic composition containing mixing extract of shipjangsaeng-related natural products (pine sprouts, ganoderma lucidum mushroom, arbutin, deer antler, Zara, germanium, royal jelly, red ginseng, salmon roe and lemon) natural substance (Pinus sylvestris, Ganoderma lucidum Karst., Arbutin, Cervus elaphus L., Amyda sinensis, Germanum, Apis mellifera L., Panax ginseng CA Meyer, salmon egg, Citrus Medica Limonum)}

The present invention 10 kinds of natural materials, i.e., solssak (Pinus sylvestris), Reishi mushroom (Ganoderma lucidum Karst.), Arbutin (Arbutin), antler (Cervus elaphus L.), growing (Amyda sinensis) associated with sipjangsaeng (十長生), germanium (Germanum), royal jelly (Apis mellifera L.), in combination serves as the ginseng (Panax ginseng CA Meyer), salmon roe and wrinkle reduction, resilience enhancement and moisturizing ability recovered by containing mixed extracts of lemon (Citrus MedicaLimonum) It relates to a cosmetic composition having an effect such as skin anti-aging.

As the largest organ of our body, which occupies about 16% of our body volume, the skin is in direct contact with the external environment and serves as an important protective barrier to protect our body from many deadly harmful agents that try to break into our body. have.

However, as our skin ages, it is damaged by various contaminants and stress, which causes the cells to be damaged and cell proliferation to be lost, causing the skin to lose elasticity and wrinkles. Originally, normal healthy skin has a protective system that protects the skin from these external damage factors, but by continuous external stimulation, the protective system is destroyed, thus the skin loses its protection and cell protection and proliferation ability. Apart from this, skin aging phenomena, such as reduced skin elasticity and wrinkle formation, appear.

In addition, loss of skin's moisturizing power and loss of turn over function are also associated with wrinkle formation and skin elasticity, which is a kind of phenomenon caused by skin aging. As we age, the dermis decreases the amount of collagen, mucopolysaccharides and hyaluronic acid in the matrix, which play an important role in skin elasticity, and the amount of insoluble collagen increases. It is difficult to maintain the moisture of the skin, and the skin becomes rough. In addition, the function of the sebaceous glands and sweat glands is also reduced, so that the amount of sebum and sweat is gradually reduced, the sebaceous membrane is not properly produced, and the protection for skin moisturization is lost, and thus the skin becomes dry and translucence. Loses skin elasticity and wrinkles. In other words, the loss of moisturizing of the skin is a major symptom of aging along with decreased skin elasticity and wrinkle formation and is closely related.

Along with this, it is known that wrinkles are generated when human skin cells are exposed to ultraviolet rays (UV-B and UV-A) due to the generation of reactive oxygen species which are the main cause of skin damage.

Therefore, research has been actively conducted to develop cosmetics that can protect skin cells from the external environment such as ultraviolet light, activate cell proliferation ability, and impart smooth moisturizing power. There was no known cosmetic composition that solved all the factors of aging such as wrinkles, decreased elasticity and loss of moisturizing.

The present inventors have found that protecting skin cells from the external environment such as ultraviolet rays, activating cell proliferation ability, and at the same time smoothly moisturizing alleviate all of the fundamental causes of aging, and satisfy all of them. To develop a cosmetic that can be made. As a result, it was studied that saints, who are not supposed to die forever or lived forever, were associated with aging, and that natural extracts related to zinnia had moisturizing effects as well as cell protection and proliferation effects. The present invention has been found to effectively contribute to the prevention of total aging through resilience enhancement and moisturizing.

Therefore, an object of the present invention, by extracting the ten natural products associated with the elders, namely pine needles, ganoderma lucidum, arbutin, deer antler, grow, germanium, royal jelly, red ginseng, salmon roe and lemon and introduce it into cosmetics, The extract effectively contributes to skin wrinkle relief, elasticity enhancement and moisturizing recovery through cell protection from UV rays, cell proliferation and excellent moisturizing properties, and provides a cosmetic composition having no problems with safety and stability.

Figure 1 shows the skin elasticity test results of the nutrition cream containing the extract of the jangsaengeng of the present invention.

Figure 2 shows the results of the moisturizing effect test of nutrient lotion containing the mixed extract of the elders of the present invention.

In order to achieve the above object, the cosmetic composition according to the present invention is dried natural extracts related to the elders, that is, dried extracts of pine needles, Ganoderma lucidum, arbutin, antler, Zara, germanium, royal jelly, red ginseng, salmon roe and lemon It is characterized by containing in an amount of 0.01 to 7% by weight as weight.

In addition, the preferred dry weight ratio of each of the 10 extracts to the mixed extract is 5-15% pine needles, 10-15% Ganoderma lucidum mushroom, 1-5% arbutin, 15-25% antler, 15-20%, germanium 0.0001- 0.0003%, royal jelly 10-15%, red ginseng 5-15%, salmon eggs 3-10% and lemon 10-15% is good.

The present inventors have studied with regard to the elders, meaning longevity and eternal life, in order to overcome the aging of the human skin, wrinkles, loss of elasticity and moisturizing ingredients with age. In other words, the elders of ten water creatures consisting of pine, bullocho, crane, deer, turtle, rock, water, mountain, sun and cloud are long-lived and long-lived. This is related to aging.

Thus, in the present invention, as a natural product associated with the elders, pine needles, ganoderma lucidum mushroom, arbutin, antler, Zara, germanium, royal jelly, red ginseng, salmon roe and lemon were used. The pine, deer, and turtle that can be obtained directly from the elders used pine needles, deer antler, and jarra by themselves. In other words, Bulchocho was selected and used as a territories with anti-wrinkle and immune-boosting effects in the sense that it does not grow old, and the crane symbolized nobleness and cleanness, and the corresponding effect was found in the arbutin used as a whitening agent. In addition, germanium was selected to enhance skin resistance as a means of rock strength and firmness. The water moisturizing effect could be exhibited by using royal jelly, which has moisturizing and elasticity effects. Red ginseng, known for its antioxidant and anti-aging effects, can be combined with intense energy or cell regeneration, and salmon roe with corresponding efficacy, and the circulatory function of the cells Lemons were selected in connection with keratinization. The above ten natural products are natural products having different effects, and are related to the elders. The present inventors introduced the mixed extracts obtained from the above natural products to the skin, thereby moisturizing them together with protecting the cells from ultraviolet rays and proliferating the cells. Even the action was confirmed to be effective.

Accordingly, the cosmetics were prepared by blending the above extracts of a certain concentration of the elders, and when used directly on human skin, compared to the conventional anti-wrinkle or moisturizing cosmetics, in the functional aspects such as skin elasticity enhancement, wrinkle improvement and moisturizing recovery In this case, it shows a distinctly excellent effect, and the above effects are combined to provide an anti-aging effect, as well as excellent skin stability and safety, and completed the present invention.

The natural product, that is, the method of producing a mixed extract of pine needles, ganoderma lucidum mushroom, arbutin, deer antler, grow, germanium, royal jelly, red ginseng, salmon roe and lemon is as follows. 5-15% of pine needles, 10-15% of Ganoderma lucidum mushrooms, 1-5% of arbutin, 15-25% of antler, 15-20% of growth, germanium 0.0001-0.0003%, royal jelly 10-15%, red ginseng 5-15%, One or more solvents selected from the group consisting of water, ethanol, methanol, butanol, ethyl acetate, butylene glycol, propylene glycol, and glycerin as extracting solvents, which are mixed at a dry weight ratio of 3 to 10% salmon and 10 to 15% lemon To 5 to 15 times the volume of the mixed extract is added. Extraction method is a method of extracting by heating at 50 to 95 ℃ for 3 to 20 hours, or by dipping at 5 to 37 ℃ for 1 to 15 days in a state that the cooling condenser is installed to prevent evaporation of the solvent This is available. In addition, since the same effect is obtained even when each of the natural product extract and then mixed with each other, this method is equally applicable. Using a distillation apparatus equipped with a cooling condenser to recover the evaporated solvent, concentrated concentrated extracts of natural products from pine needles, ganoderma lucidum, arbutin, antler, zara, germanium, royal jelly, red ginseng, salmon roe and lemon, The dry weight thereof is 0.01 to 7%, preferably 0.3 to 5%, and added to the cosmetic. If the added amount is less than 0.01%, it is not preferable to show the effectiveness, if more than 7% there is a problem in formulating, the optimum concentration considering the efficacy and stability is 0.3 ~ 5%.

Hereinafter, the configuration and effects of the present invention will be described in more detail with reference to Examples, Examples, and Test Examples, but the content of the present invention is not limited thereto.

[Reference Example 1] Preparation of natural eleven extracts

Complete drying, consisting of 15% pine needles, 10% Ganoderma lucidum mushroom, 2.9999% arbutin, 15% antler, 15% Zara, 0.0001% germanium, royal jelly 10%, red ginseng 13%, salmon roe 5% and lemon 12% 1 kg of the mixed mixture of the raw materials was placed in 10kg of water, extracted by boiling for 3 hours in an extractor equipped with a cooling condenser, filtered through a 400 mesh filter cloth and cooled to room temperature. After aging for 5 days at 5 ~ 15 ℃, it was filtered with Whatman No. 2 filter paper. The filtrate was concentrated under reduced pressure at 70 ° C. in a distillation apparatus equipped with a cooling condenser to obtain 28 g of a natural product extract (dry weight).

Reference Example 2 Preparation of Elderberry Natural Mixed Extract

43 g (dry weight) of the natural product extract was obtained using the same method as in Reference Example 1, except that 10 kg of an aqueous butylene glycol solution (30%) was used instead of 10 kg of the water used as the solvent in Reference Example 1.

[Reference Example 3] Preparation of natural mixed extract of elders

37 g (dry weight) of the natural product extract was obtained using the same method as in Reference Example 1, except that 10 kg of butylene glycol was used instead of 10 kg of water used as the solvent in Reference Example 1.

[Reference Example 4] Preparation of natural eleven extracts

33 g (dry weight) of the natural product extract was obtained using the same method as in Reference Example 1, except that 10 kg of ethanol aqueous solution (30%) was used instead of 10 kg of water used as the solvent in Reference Example 1.

Reference Example 5 Preparation of Elderberry Natural Mixed Extract

25 g (dry weight) of natural product extract was obtained using the same method as Reference Example 1, except that 10 kg of ethanol was used instead of 10 kg of water used as the solvent in Reference Example 1.

[Reference Example 6] Preparation of natural mixed extract of elders

29 g (dry weight) of the natural product extract was obtained using the same method as in Reference Example 1, except that 10 kg of butanol was used instead of 10 kg of water used as the solvent in Reference Example 1.

Reference Example 7 Preparation of Elderberry Natural Mixed Extract

Using the same method as in Reference Example 1, except that 10 kg of ethyl acetate was used instead of 10 kg of water used as the solvent in Reference Example 1, 30 g (dry weight) of the natural product extract was obtained.

Reference Example 8 Preparation of Elderberry Natural Mixed Extract

32 g (dry weight) of a natural product extract was obtained using the same method as in Reference Example 1, except that 10 kg of methanol was used instead of 10 kg of water used as the solvent in Reference Example 1.

Reference Example 9 Preparation of Elderberry Natural Mixed Extract

36 g (dry weight) of the natural product extract was obtained in the same manner as in Reference Example 1, except that 10 kg of an aqueous solution of propylene glycol (30%) was used instead of 10 kg of water used as the solvent in Reference Example 1.

Reference Example 10 Preparation of Elderberry Natural Mixed Extract

31 g (dry weight) of a natural product extract was obtained using the same method as in Reference Example 1, except that 10 kg of aqueous glycerin solution (30%) was used instead of 10 kg of water used as the solvent in Reference Example 1.

Test Example 1 Cell Protection Effect Test from Ultraviolet Rays

1) test method

Human fibroblasts were placed in well plates at 2 × 10 ⁵ cells / well and 0.2 ml of DMEM medium containing 10% FBS was supplied, followed by incubation in a CO ₂ incubator for 24 hours. After treating each of the natural product mixed extracts of Reference Examples 1 to 10 dissolved in DMSO, and then irradiated with ultraviolet rays of 2MED for 5 minutes, it was incubated for 24 hours. MTT assay was performed to determine the viability of the cells. In the MTT assay, 0.1 ml of MTT solution was added to the cultured cells, followed by 4 hours of incubation. The medium was removed, and 0.5 ml of DMSO was added to formazan. It was. In addition, absorbance was measured as a control, which was tested with DMSO, a solvent in which the solid content of each reference example was dissolved, and the cell protective effect was determined from the difference in relative absorbance with the control. On the other hand, the effect was measured for the green tea extract which is a representative raw material for skin aging for comparison.

2) Test result

According to the method described above, the effect of protecting the cells from ultraviolet rays of the mixed extract of the present invention of the present invention was measured, and the results are shown in Table 1. From the above results, it can be seen that the extract of the present invention has a significantly greater protective effect when compared with the green tea extract, which is a representative raw material for skin aging.

Concentration of the sample contained in the reaction solution (µg / ml) Cell protective effect (%) Soy Sauce Natural Blend Extract Green tea extract Reference Example 1 100 71 (0.80847) 68 (0.78584) Reference Example 2 100 85 (0.96806) 79 (0.8997) Reference Example 3 100 79 (0.8996) 70 (0.79723) Reference Example 4 100 73 (0.83139) 62 (0.70612) Reference Example 5 100 45 (0.51251) 42 (0.47834) Reference Example 6 100 59 (0.81951) 46 (0.63894) Reference Example 7 100 75 (0.8542) 64 (0.72889) Reference Example 8 100 63 (0.71750) 50 (0.56945) Reference Example 9 100 67 (0.76306) 47 (0.53528) Reference Example 10 100 69 (0.78584) 59 (0.67195) Cytoprotective effect (%) = absorbance of the test group / absorbance of the control group × the number in the parenthesis is the absorbance (nm). For reference, the absorbance of the control group is 1.0449 nm

[Test Example 2] Cell protective effect test from Superoxide radical

1) test method

Human fibroblasts were placed in well plates with 2 × 10 ⁵ cells / well and 0.2 ml of DMEM medium containing 10% FBS was supplied, followed by incubation in a CO ₂ incubator for 24 hours. Here, each of the natural product mixed extracts of Reference Examples 1 to 10 dissolved in DMSO was treated and incubated for 30 minutes. 10 μl of 1.5 mM xanthine and 10 μl of 0.25 U xanthin oxidase were added to the cultured cells to generate superoxide radicals, and then cultured in a CO ₂ incubator for 24 hours. MTT assay was performed to determine the viability of the cells. In the MTT assay, 0.1 ml of MTT solution was added to the cultured cells, followed by 4 hours of incubation. The medium was removed, and 0.5 ml of DMSO was added thereto. The absorbance was measured at 570 nm using an ELISA reader. In addition, absorbance was measured as a control, which was tested with DMSO, a solvent in which the solid content of each reference example was dissolved, and the cell protective effect was determined from the difference in relative absorbance with the control. On the other hand, the effect was measured for the green tea extract which is a representative raw material for skin aging for comparison.

2) Test result

According to the above method, the effect of protecting the cells from the superoxide radicals of the mixed extract of the eleven years of the present invention was measured, and the results are shown in Table 2. From the above results, it can be seen that the extract of the present invention also exhibits an excellent cell protection effect from super oxide radicals, as compared to the green tea extract which is a representative raw material for skin aging.

Concentration of the sample contained in the reaction solution (µg / ml) Cell protective effect (%) Soy Sauce Natural Blend Extract Green tea extract Reference Example 1 100 73 (0.76278) 69 (0.70008) Reference Example 2 100 91 (0.95086) 82 (0.85682) Reference Example 3 100 85 (0.88816) 77 (0.80457) Reference Example 4 100 81 (0.84637) 76 (0.79412) Reference Example 5 100 63 (0.65829) 60 (0.62694) Reference Example 6 100 79 (0.82547) 66 (0.68963) Reference Example 7 100 83 (0.86727) 78 (0.81502) Reference Example 8 100 53 (0.5580) 46 (0.48065) Reference Example 9 100 87 (0.90906) 69 (0.72098) Reference Example 10 100 75 (0.78367) 71 (0.74188) Cytoprotective effect (%) = absorbance of the test group / absorbance of the control group × the number in the parenthesis is the absorbance (nm). For reference, the absorbance of the control group is 1.0449 nm

Test Example 3 Cell Proliferation Effect Test

1) test method

100 μl of 5% fetal bovine serum (FBS) solution was added to a 96 μ -well plate, and each of the natural product mixed extracts of Reference Examples 1 to 10 was dispersed in a predetermined concentration in DMEM medium containing 2% FBS. Diluted and added again 100 μl. Fibroblasts obtained from the foreskin tissue of newborns were added to a 4000 μl per well in 100 μl medium using a hemocytometer, and then cultured for 7 days. After the incubation, the supernatant was removed and washed again by adding 200 μl of 5% phosphate buffered saline (PBS), followed by 150 μl of DMSO per well and shaking for 10 minutes to measure absorbance at 570 nm. In addition, absorbance was measured as a control, which was tested with DMSO, a solvent in which the solid content of each reference example was dissolved, and the cell proliferation effect was determined from the difference in relative absorbance with the control. On the other hand, the effect was measured for the green tea extract which is a representative raw material for skin aging for comparison.

2) Test result

According to the above method, the cell proliferation effect of the E. coli extract of the present invention was measured, and the results are shown in Table 3. From the above results, it can be seen that the extract of the present invention exhibits excellent cell proliferation effect when compared to the green tea extract, which is a representative raw material for skin aging.

Concentration of the sample contained in the reaction solution (µg / ml) Cell proliferation effect (%) Elders Natural Blend Extract Green tea extract Reference Example 1 100 103 (1.25804) 101 (1.23361) Reference Example 2 100 127 (1.55119) 109 (1.33132) Reference Example 3 100 111 (1.35575) 110 (1.34354) Reference Example 4 100 121 (1.47789) 115 (1.40461) Reference Example 5 100 102 (1.24583) 102 (1.24583) Reference Example 6 100 113 (1.38018) 104 (1.27026) Reference Example 7 100 115 (1.40460) 103 (1.258042) Reference Example 8 100 107 (1.30689) 105 (1.28247) Reference Example 9 100 116 (1.41682) 108 (1.31911) Reference Example 10 100 109 (1.33132) 102 (1.24583) Cell proliferation effect (%) = absorbance of test group / absorbance of control group × 100 Figure in parentheses is absorbance (nm).

Test Example 4 Moisturizing Effect Test

1) test method

5 g of the natural product mixed extract of Reference Examples 1 to 10 was added to a dish, which was left under conditions of 41% relative humidity and 25 ° C. in a desiccator containing CaCl ₂ , and then a change in weight after a certain period of time was observed. Measurement was made over time. As a comparative example, 1,3-butylene glycol generally used in moisturizing cosmetics was used.

2) Test result

According to the above method, the moisturizing effect of the mixed extract of the elders of the present invention was measured, and the results are shown in Table 4. From the above results, the extract of the present invention showed a high moisture content due to moisturizing, and it was confirmed that the effect was better when compared to 1,3-butylene glycol known as a moisturizing substance.

Measurement time (hr) Moisture Content (%) Reference Example 1 120 47.2 Reference Example 2 120 53.5 Reference Example 3 120 50.7 Reference Example 4 120 47.7 Reference Example 5 120 45.5 Reference Example 6 120 49.3 Reference Example 7 120 48.5 Reference Example 8 120 46.6 Reference Example 9 120 49.8 Reference Example 10 120 51.5 Comparative Example (containing 30% of 1,3-butylene glycol) 120 43.2 Moisture Content (%) = Residual Sample Volume (g) / Initial Sample Volume (g) × 100

Test Example 5 Safety Test of Natural Mixed Extracts

In order to test the safety of the natural mixed extracts of the elders, a primary skin irritation test, a primary eye mucosal irritation test and a skin sensitization test were conducted on experimental rats and the results are shown in Tables 5 to 7 below (test period). : January 4, 2001 ~ April 20, 2001, test organization: Bioland Biotechnology Research Institute. Table 5 below shows the results of the primary skin irritation test.

time Hong Van Edema 30-60 minutes 0 0 24 hours 0 0 48 hours 0 0 72 hours 0 0 * Experimental animal species: Albino Rabbit from New Zealand, age and weight range at the start of administration: 10 weeks, 2.5㎏ ± 20g

From the above results, it was confirmed that the eleven natural extract of the present invention is a safe substance without irritation such as skin erythema or edema.

In addition, Table 6 below shows the results of the primary eye mucosal irritation test.

opinion 1 hours 24 hours 48 hours 72 hours Corneal haze 0 0 0 0 Over iris 0 0 0 0 Corneal Redness Edema 0 0 0 0 * Experimental animal species: Albino Rabbit from New Zealand, age and weight range at the start of administration: 10 weeks, 2.5㎏ ± 20g

From the above results, it was confirmed that the natural extract of the eleven years of the present invention is a safe substance without eye mucosal irritation such as corneal haze, iris abnormalities or corneal red edema.

In addition, Table 7 below shows the results of the primary skin sensitization test.

time Reduction rate (%) Rating Sensitization degree 24 hours 6 I Very weak 48 hours 3 I Very weak * Experimental animal species: Hartly male Guinea-Pig, * Age and weight range at the start of administration: 10 weeks, 300-500 g

In the above results, the sensitization rate is calculated in the usual manner from the number of animals that showed a positive response to the test and their skin reactions scored, and when the sensitization rate is 0 to 8%, grade I Note that it is common to set the degree of sensitivity to very weak. Therefore, it was confirmed that the natural extract of the elders of the present invention is a safe substance for skin sensitization.

In addition, in the human skin patch test, all reactions were negative for 30 minutes and 24 hours after sample removal, and no abnormality was confirmed even on day 7 after sample removal.

That is, from the above safety test results, it was proved that the natural extracts of the zinnia were very safe substances.

[Test Example 6] Test for measuring stability of physical properties of natural mixed extracts

In order to evaluate how stable the physical properties of natural extracts can be used with cosmetics, the discoloration measurement, which is generally used to measure the physical properties of cosmetic ingredients, was performed at room temperature, 40 ° C and sunlight. The results are shown in Table 8 below.

Date condition One 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Room temperature - - - - - - - - - - - - - - - 40 ℃ - - - - - - - - - - - - - - - daylight - - - - - - - - - - - - - - - Date condition 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 Room temperature - - - - - - - - - - - - - - - 40 ℃ - - - - - - - - - - - - - - - daylight - - - - - - - - - - - + + + + -; No color change, +; Start discoloration, ++; Changing a lot, +++; Complete discoloration

From the above results, the mixed extract of the present invention was found to be very stable at room temperature and 40 ° C, and almost negligible discoloration in daylight, but it was a very stable material without further discoloration progression.

Test Example 7 Test for Increased Elasticity of Human Skin

To prepare the nutrition cream of Example 1 formulated by containing the natural extract of the Elderberry of Reference Example 2 and the nutrition cream of Comparative Example 1 containing a seaweed extract that has been conventionally used in cosmetics as an elasticity increasing substance, when applying the skin The effect on the elasticity of the skin was measured. In the test, 20 women aged 20 to 55 years had normal, oily, dry, and complex skin. The nutrition cream of Example 1 was applied to the left side of the face, and the nutrition cream of Comparative Example 1 was applied to the right side of the face. After application twice a day to use in the evening, after 1 week, 2 weeks, 3 weeks and 4 weeks was carried out by a method of measuring the elasticity of the skin using a skin elastic meter (Ballisto meter). The results are shown in Table 9 and FIG. 1.

Example 1 and Comparative Example 1 Nutritional Cream

Raw material Example 1 Comparative Example 1 Naturally Mixed Extract of Elderberry (Reference Example 2) 7.0 - Seaweed extract - 7.0 Cytostolol 4.0 4.0 Polyglyceryl 2-oleate 3.0 3.0 Ceramide 0.7 0.7 Ceteares-4 2.0 2.0 cholesterol 3.0 3.0 Dicetylphosphate 0.4 0.4 Concentrated glycerin 5.0 5.0 Sunflower oil 22.0 22.0 Carboxy Vinyl Polymer 0.5 0.5 Triethanolamine 0.5 0.5 antiseptic a very small amount a very small amount Spices a very small amount a very small amount Purified water Remaining amount Remaining amount

As can be seen from the above results, the nutritional cream of Example 1 containing the natural mixed extract according to the present invention has improved skin condition compared to the nutritional cream of Comparative Example 1 containing seaweed extract, which was previously used as a material for increasing skin elasticity. It appeared that the skin elasticity effect by.

Test Example 8 Wrinkle Improvement Effect Test

For the nutritional essence of Example 2 formulated by containing the natural extract of the Elderberry of Reference Example 2 and the nutritional essence of Comparative Example 2 containing hyaluronic acid conventionally used in wrinkle improvement cosmetics, wrinkle improvement according to skin elasticity enhancement In order to measure the effect, Example 2 was applied twice a day to the left eye edge of 20 people, and Comparative Example 2 was applied to the right eye edge. After 45 days after each treatment, the elasticity of the skin surface was measured by Cutometer SEM 474. Skin elasticity measurement by the cutometer SEM 474 is to measure the depth of wrinkles, the smaller the value is the elasticity is good and the wrinkles are relaxed. The elasticity value was expressed as a percentage decrease compared to the measured value before treatment, and the average value of 20 subjects was obtained and shown in Table 10.

Example 2 and Comparative Example 2 Nutritional Essence

Raw material Example 2 Comparative Example 2 Naturally Mixed Extract of Elderberry (Reference Example 2) 7.0 - Hyaluronic acid - 7.0 Cytostolol 1.70 1.70 Polyglyceryl 2-oleate 1.50 1.50 Ceramide 0.7 0.7 Ceteares-4 1.2 1.2 cholesterol 1.5 1.5 Dicetylphosphate 0.4 0.4 Concentrated glycerin 5.0 5.0 Sunflower oil 15.0 15.0 Carboxy Vinyl Polymer 0.2 0.2 Xanthan Gum 0.2 0.2 antiseptic a very small amount a very small amount Spices a very small amount a very small amount Purified water Remaining amount Remaining amount

sample Skin elasticity (wrinkle depth reduction) effect (%) 30 days 45 days Example 2 17.7 29.3 Comparative Example 2 10.4 21.5

Test Example 9 Moisturizing Effect Test

Regarding the nutrition lotion of Example 3, which was formulated by containing the natural extract of Elderberry of Reference Example 2, and the nutrition lotion of Comparative Example 3 containing 1,3-butylene glycol, which was generally used in moisturizing cosmetics, Example 3 and Comparative Example 3 were applied to the inside of the left arm of 20 people to test the skin moisturizing effect. After each application, the moisture on the skin surface was measured over time by Corneometer CM 825. Skin moisture measurement by Corneometer CM 825 is a measure of the moisture content of the skin, the higher the value the better the moisture. The average value of 20 subjects was obtained and shown in Table 11 and FIG. 2. The test was carried out at a temperature of 20 ° C. and a relative humidity of 20%.

Example 3 and Comparative Example 3

Raw material Example 3 Comparative Example 3 Naturally Mixed Extract of Elderberry (Reference Example 2) 7.0 - 1,3-butylene glycol - 7.0 Cytostolol 1.70 1.70 Polyglyceryl 2-oleate 1.50 1.50 Ceramide 0.7 0.7 Ceteares-4 1.2 1.2 cholesterol 1.5 1.5 Dicetylphosphate 0.4 0.4 Concentrated glycerin 5.0 5.0 Sunflower oil 10.0 10.0 Carboxy Vinyl Polymer 0.2 0.2 Xanthan Gum 0.3 0.3 antiseptic a very small amount a very small amount Spices a very small amount a very small amount Purified water Remaining amount Remaining amount

Time (min) 5 10 15 20 25 30 Example 3 19.4 7.98 5.88 5.16 4.18 4.46 Comparative Example 3 20.4 7.62 4.12 3.74 3.62 3.02

Hereinafter, the cosmetics containing the natural extract of the elders of the present invention is shown as an example of the formulation, but the present invention is not limited to these formulations.

Formulation Example 1 Lotion (Skin Lotion)

Raw material Formulation Example 1 Elders Natural Blend Extract 7.0 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Polyoxyethylene (60) Cured Castor Oil 1.0 ethanol 10.0 Triethanolamine 0.1 antiseptic a very small amount Pigment a very small amount Spices a very small amount Purified water Remaining amount

Formulation Example 2 Massage Cream

Raw material Formulation Example 2 Elders Natural Blend Extract 7.0 Beeswax 10.0 Polysorbate 60 1.5 Solbitan Sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Caprylic / Capric Triglycerides 4.0 Carboxy Vinyl Polymer 0.2 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 antiseptic a very small amount Pigment a very small amount Spices a very small amount Purified water Remaining amount

By extracting 10 natural products related to the elders, namely pine needles, ganoderma lucidum, arbutin, deer antler, grow, germanium, royal jelly, red ginseng, salmon roe and lemon and introducing them into cosmetics, the cells from the ultraviolet rays of the extract Through protective, cell proliferative and excellent moisturizing properties, it is possible to provide an effect such as skin anti-aging, which acts in combination with skin wrinkle relief, elasticity enhancement and moisturizing recovery.

Claims (8)

0.01 ~ 7% by weight of the mixed extract obtained by mixing and extracting 10 kinds of natural products related to the elders, pine needles, ganoderma lucidum, arbutin, deer antler, grow, germanium, royal jelly, red ginseng, salmon roe and lemon. Cosmetic composition characterized in that it contains in proportion. According to claim 1, wherein the content ratio of the natural products in each dry weight ratio of 5 to 15% pine needles, Ganoderma lucidum mushroom 10 to 15%, arbutin 1 to 5%, deer antler 15 to 25%, growing 15 to 20%, germanium 0.0001 to Cosmetic composition, characterized in that 0.0003%, royal jelly 10-15%, red ginseng 5-15%, salmon roe 3-10% and lemon 10-15%. The cosmetic composition according to claim 1, wherein the natural product mixed extract is extracted by dipping in a solvent at 5 to 37 ° C for 1 to 15 days. According to claim 1, wherein the natural product extract is a cosmetic composition, characterized in that extracted by heating for 3 to 20 hours at 50 ~ 95 ℃ to add a solvent. The cosmetic composition according to claim 3, wherein the solvent used for extraction is at least one selected from the group consisting of water, ethanol, methanol, butanol, ethyl acetate, butylene glycol, propylene glycol and glycerin. The cosmetic composition according to claim 1, wherein the extract is used for protecting cells from ultraviolet rays, for proliferating cells, or for moisturizing them. The cosmetic composition according to claim 1, wherein the cosmetic is formulated as a lotion (skin lotion), nutrition lotion, nutrition cream, massage cream or nutrition essence. Pine Needle Extract 5-15%, Ganoderma lucidum Extract 10-15%, Arbutin Extract 1-5%, Deer Antler Extract 15-25%, Grow Extract 15-20%, Germanium Extract 0.0001-0.0003%, Royal Jelly Extract 10-15% , Red ginseng extract 5-15%, salmon roe extract 3-10% and lemon extract 10-15% of a mixture of natural extracts comprising a dry weight of 0.01 to 7% by weight in a cosmetic composition.