CN107550847B - Active skin care composition and application thereof - Google Patents
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Abstract
The invention relates to an active skin care composition and application thereof, wherein the skin care composition comprises the following components in parts by weight: 1-10 parts of natural pigment ethosome, 0.05-4 parts of glycyrrhetinic acid, 0.05-2 parts of palmatine, 0.1-1 part of cysteinylglycine, 0.1-1 part of L-ascorbyl palmitate, 0.01-1 part of laccaic acid and 1-10 parts of freeze-dried powder of mountain rose callus cell suspension culture solution. The active skin care composition aims to ensure that natural ingredients are in close contact with skin, so that double effects of beautifying external skin and regulating internal management are achieved, the common requirements of different skin forms such as whitening, allergy prevention, oil control, aging resistance, skin tendering and the like are met, and the skin care can immediately present an astonishing effect.
Description
Technical Field
The invention relates to the technical field of cosmetics, and particularly relates to an active skin care composition and application thereof.
Background
It is well known that ultraviolet rays promote skin aging, and excessive ultraviolet irradiation destroys dermal elastic fibers and collagen fibers, causing fibrosis to break, resulting in skin relaxation and wrinkles. At the same time, the ultraviolet light also releases signals for activating melanin formation, and excess melanin is produced, so that the skin is hyperpigmented, forms color spots, and is accompanied with the loss of skin luster and becomes dull. Meanwhile, the damage of ultraviolet rays to the stratum corneum of the skin promotes free radicals generated by the metabolism of epidermal and dermal cells, the free radicals are the main cause of oxidation of the body, the membranous substances of the cells are damaged, so that the loss of water is caused, the skin secretes more grease due to the loss of a large amount of water, dirt such as dust is more difficult to wash away, and the problems of dark yellow skin color, blackheads, rough skin and the like can be caused after the skin wears out.
At present, skin care products on the market are various in types, but have different qualities, good effects and single effects, and are mainly used for moisturizing, whitening and removing freckles, resisting aging or removing wrinkles. And some products also contain lead, mercury, hormone and other components, which not only can not play a role in skin care, but also seriously threaten the health of the skin, and some skin care products have larger water content or contain alkalescent components, and can cause skin edema and allergy phenomena due to excessive infiltration when contacting the skin for a long time, so that the current purely natural skin care products are deeply trusted and loved by consumers.
Chinese patent application with publication No. CN 104758222B discloses a skin care product with effects of repairing and regenerating skin, replenishing water, keeping moisture and delaying aging, which comprises Atractylodis rhizoma extract, radix Ampelopsis extract, radix Angelicae sinensis extract, semen Armeniacae amarum extract, herba Dendrobii extract, Aloe extract, Bombyx Batryticatus powder and rhizoma Bletillae powder, and has effects of whitening skin, removing speckle, preventing and resisting allergy and delaying cell aging.
Also, as disclosed in chinese patent application No. CN 104622776B, "a cosmetic composition containing Chinese medicinal extracts" comprises ginseng, angelica sinensis, snow lotus herb, argy wormwood leaf, notoginseng, frankincense, eupatorium, pseudostellaria root, agrimony, fragrant solomonseal rhizome, pilose antler, gentian, magnolia flower, selaginella tamariscina, peony, centella asiatica, patchouli, chuanxiong rhizome, poria cocos, aucklandia root, chamomile, and lycium barbarum, and has good effects of whitening skin, removing freckles, tendering skin, and removing wrinkles.
However, the skin care compositions all contain a large number of traditional Chinese medicine components, which greatly increases the difficulty in compounding, and is difficult to ensure that the skin care products maintain good stability in the long-term use process, so that a natural plant sunscreen skin care product with stable quality and remarkable skin care effect is needed to be provided.
Disclosure of Invention
In order to solve the problems in the prior art, the invention aims to provide an active skin care composition and application thereof, wherein the skin care composition has stable property, is safe, non-irritant, mild and skin-friendly, has better sun-screening performance, has good repairing effect on injuries such as dryness, desquamation and redness and swelling after sun-drying, has obvious effects of moisturizing, soothing allergy, whitening and removing freckles, and has a certain effect on delaying senility.
The technical scheme of the invention is as follows:
the active skin care composition is characterized by comprising the following components in parts by weight:
1-10 parts of natural pigment ethosome, 0.05-4 parts of glycyrrhetinic acid, 0.05-2 parts of palmatine, 0.1-1 part of cysteinyl glycine and 0.1-1 part of L-ascorbyl palmitate.
Preferably, the skin care composition further comprises 0.01-1 part of laccaic acid.
Preferably, the skin care composition further comprises 1-10 parts of mountain rose callus cell suspension culture solution freeze-dried powder.
More preferably, the skin care composition comprises the following components in parts by weight:
5-8 parts of natural pigment ethosome, 1-2 parts of glycyrrhetinic acid, 0.1-1 part of palmatine, 0.5-1 part of cysteinylglycine, 0.5-1 part of L-ascorbyl palmitate, 0.1-0.5 part of laccaic acid and 6-10 parts of freeze-dried powder of mountain rose callus cell suspension culture solution.
Further, the natural pigment ethosome comprises the following components in parts by weight: 0.1-1 part of natural pigment, 4-12 parts of soybean lecithin, 20-30 parts of ethanol, 1-2 parts of polyethylene glycol stearate, 3-5 parts of hydroxyethyl piperazine ethanesulfonic acid and 20-30 parts of deionized water.
Preferably, the natural pigment ethosome comprises the following components in parts by weight: 0.8 part of natural pigment, 8 parts of soybean lecithin, 30 parts of ethanol, 2 parts of polyethylene glycol stearate, 5 parts of hydroxyethyl piperazine ethanesulfonic acid and 25 parts of deionized water.
Further, the natural pigment is composed of chlorophyll and lycopene according to the mass ratio of 1: 0.1-1.
Preferably, the natural pigment consists of chlorophyll and lycopene in a mass ratio of 1: 0.6.
In the active skin care composition, the chlorophyll and the lycopene are natural fat-soluble pigments and have light absorption performance, and the chlorophyll and the lycopene have better synergistic effect on the absorption of ultraviolet rays. However, natural pigments tend to be poor in stability and difficult to dissolve in water, greatly limiting their use, and have a certain color, making the pigments difficult to apply to the preparation of skin care products. According to the invention, chlorophyll and lycopene are creatively prepared into an ethosome, and the chlorophyll and lycopene are coated in the ethosome by utilizing an ethosome bilayer structure, so that the solubility and stability of the ethosome and the ethosome are greatly improved, the degradation effect of external factors on the chlorophyll and the lycopene is avoided, the absorption effect of the chlorophyll and the lycopene on visible light and ultraviolet light is fully exerted, and a better sun-screening effect is obtained. Meanwhile, the ethosome is easy to deform to penetrate a skin barrier, so that transdermal absorption is promoted, the capability of chlorophyll and lycopene entering cells is enhanced, and the effects of scavenging free radicals, resisting oxidation, delaying aging and improving immunity are fully exerted.
Specifically, the preparation of the natural pigment ethosome comprises the following steps:
weighing soybean lecithin, dissolving with ethanol, adding polyethylene glycol stearate and natural pigment, and mixing; at 400r.min-1Under the condition of (1), using a peristaltic pump at 100 mu L.min-1The pre-dissolved water solution of the hydroxyethyl piperazine ethanesulfonic acid is injected into the ethanol solution at the speed of 10000r.min-1Stirring for 2min to obtainHomogenizing, treating with 200nm microporous homogeneous membrane to obtain alcoholic body fluid, cooling, and solidifying to obtain natural pigment ethosome.
In the preparation process of the natural pigment ethosome, the polyethylene glycol stearate can promote the ethosome to form complete and uniform round-like vesicles under the buffering condition of the ethylene sulfonic acid containing the hydroxyethyl piperazine, reduce the particle size of the ethosome and improve the encapsulation rate of chlorophyll and lycopene.
Further, the invention also provides a preparation process of the mountain rose callus cell suspension culture solution freeze-dried powder, which comprises the following steps:
(1) inoculating sterile mountain rose leaves into a solid culture medium of MS + 0.8-1.2 mg/L6-BA + 1.2-2.0 mg/L2, 4-D +4g/L agar +25g/L sucrose to induce callus, wherein the subculture time is 105-120 days, and selecting loose pale yellow white callus which is subcultured for 3 times continuously;
(2) taking the loose pale yellow white callus obtained in the step (1), cutting the callus into mung bean size by using sterilized forceps, inoculating the mung bean size into a 100ml conical flask containing 40ml of 1/2MS + 1.5-2.0 mg/L6-BA + 0.5-1.0 mg/L2, 4-D +30g/L sucrose and a liquid culture medium with pH of 5.8, carrying out suspension culture on the conical flask at the constant temperature of 25 ℃ on a shaking bed at 100rpm under the condition of 12h/D illumination time, after culturing for 15D, filtering the callus by using a 50-mesh cell sieve, taking filtrate, filtering the filtrate by using an 80-mesh cell sieve, taking the filtrate, sterilizing and freeze-drying to obtain the callus.
In addition, the invention also provides application of the active skin care composition in preparing skin care products or cosmetics with the functions of sun screening, repairing, skin aging delaying, whitening and freckle removing.
The active skin care composition reasonably compounds ethosome containing chlorophyll and lycopene, glycyrrhetinic acid, palmatine, cysteinyl glycine and L-ascorbyl palmitate, and the components have synergistic effect, can effectively absorb ultraviolet rays and remove free radicals, prevent the damage of the ultraviolet rays to skin, reduce the ultraviolet rays to promote fiber cell apoptosis, delay the skin photoaging effect, delay the reproduction of melanocytes and inhibit the generation of melanin, and has the effects of whitening and removing freckles.
The active skin care composition provided by the invention also contains one or two combinations of laccaic acid and mountain rose callus cell suspension culture solution freeze-dried powder, and the two components are mutually cooperated with other components of the active skin care composition to promote the repair of elastic fibers and collagen fibers and have a repair effect on photochemical damage. The laccaic acid can promote blood circulation, activate skin, improve skin color and keep skin luster, and the laccaic acid interacts with the L-ascorbyl palmitate to greatly improve the stability of the natural pigment ethosome, so that the skin care lotion containing the natural pigment ethosome is kept in a good dispersion state in the long-term use process, and chlorophyll and lycopene are prevented from being demulsified and separated out to form precipitates. The mountain rose callus cell suspension culture solution freeze-dried powder can promote the synthesis of collagen and hyaluronic acid, improve the hydration degree of skin, enable the skin to have a good moisturizing effect, tighten the skin, resist immunosuppression caused by IL-10 expression increase caused by ultraviolet light, stimulate the natural repair matrix of skin DNA after ultraviolet irradiation, promote the DNA synthesis of cells damaged by ultraviolet irradiation, obviously reduce sunburn of cells induced by ultraviolet light, and delay the skin aging process.
Compared with the prior art, the invention has the advantages that:
(1) the active skin care composition provided by the invention is stable in property, can play the effects of moisturizing, anti-allergy, wrinkle removal, antioxidation, whitening, freckle removal and the like when being added into skin care products or cosmetics, particularly has an obvious defense effect on photoaging caused by ultraviolet rays, and has an obvious repair effect on sunburn damage.
(2) The active skin care composition provided by the invention aims to ensure that natural ingredients are in close contact with the skin, so that the dual effects of beautifying the external skin and regulating internal management are achieved, the common requirements of different skin forms such as whitening, allergy prevention, oil control, aging resistance, skin tendering and the like are met, and the effect of convincing the skin can be realized immediately.
Detailed Description
The present invention will be further described below by way of specific embodiments, but the present invention is not limited to only the following examples.
Example 1 preparation of Natural pigment Alosomes
Preparation of group A natural pigment ethosome:
weighing soybean lecithin, dissolving with ethanol, adding polyethylene glycol stearate and natural pigment, and mixing; at 400r.min-1Under the condition of (1), using a peristaltic pump at 100 mu L.min-1The pre-dissolved water solution of the hydroxyethyl piperazine ethanesulfonic acid is injected into the ethanol solution at the speed of 10000r.min-1Stirring for 2min to homogenize, treating with 200nm microporous homogeneous membrane to obtain alcoholic body fluid, cooling and solidifying to obtain natural pigment ethosome.
The preparation steps of the natural pigment ethosome of the groups B-E are shown by referring to the group A.
The particle size and encapsulation efficiency of the natural pigment ethosomes prepared by the groups A-E are respectively detected, and the results are shown in the following table 1:
TABLE 1 Natural pigment ethosome quality test results
| Group of | Particle size/nm | Encapsulation efficiency/% |
| Group A | 175.0 | 99.32 |
| Group B | 172.8 | 99.45 |
| Group C | 190.4 | 89.54 |
| Group D | 194.6 | 82.17 |
| Group E | 199.5 | 70.62 |
From the above table, it can be known that, in the preparation process of the ethosome, a certain amount of polyethylene glycol stearate and hydroxyethyl piperazine ethanesulfonic acid are added, so that a quasi-circular vesicle with a relatively small particle size can be effectively formed, and the encapsulation rate of chlorophyll and lycopene is obviously improved to reach more than 99%.
Example 2 preparation of mountain rose callus cell suspension culture solution lyophilized powder
(1) Inoculating sterile mountain rose leaves into a solid culture medium of MS +0.8 mg/L6-BA +1.2 mg/L2, 4-D +4g/L agar +25g/L sucrose for callus induction, wherein the subculture time is 105 days, and selecting loose pale yellow white callus for 3 successive subcultures;
(2) taking the loose pale yellow white callus obtained in the step (1), cutting the callus into mung bean size by using sterilized forceps, inoculating the mung bean size into a 100ml conical flask containing 40ml of 1/2MS +2.0 mg/L6-BA +0.6 mg/L2, 4-D +30g/L sucrose and a liquid culture medium with pH of 5.8, carrying out suspension culture on a constant temperature shaking bed at 25 ℃, carrying out 100rpm and 12h/D illumination time, after culturing for 15D, filtering by using a 50-mesh cell sieve, taking filtrate, filtering by using an 80-mesh cell sieve, taking the filtrate, sterilizing and freeze-drying to obtain the callus.
Examples 3-4 and comparative examples 1-4 skin lotions were prepared
The active skin care composition provided by the invention is used as a main raw material to prepare a skin care lotion, wherein the skin care lotions of examples 3-4 and comparative examples 1-4 comprise the preparation raw materials in parts by weight shown in the following table, and freeze-dried powder of mountain rose callus cell suspension culture solution is prepared in example 2.
Example 3 preparation of skin lotion:
dissolving palmatine and laccaic acid in water, heating while stirring until the two are completely dissolved, cooling to room temperature, sequentially adding ethylhexyl glycerol, L-ascorbyl palmitate, cysteinyl glycine, glycyrrhetinic acid, natural pigment ethosome and mountain rose callus cell suspension culture solution lyophilized powder, stirring well, and bottling.
Example 4 and comparative examples 1-4 lotions were prepared according to the methods described above.
Example 5 preparation of sunscreen cream
Example 5 preparation of sunscreen cream:
(1) mixing octadecanol, polyoxyethylene sorbitan monooleate, glyceryl monostearate, lanolin and ethylhexylglycerin, heating to 65 ℃, and stirring for dissolving to obtain phase A;
(2) dissolving palmatine and laccaic acid in water, heating while stirring until the two are completely dissolved, cooling to room temperature, sequentially adding L-ascorbyl palmitate, cysteinyl glycine, glycyrrhetinic acid, natural pigment ethosome and mountain rose callus cell suspension culture solution lyophilized powder, and stirring to obtain phase B;
(3) slowly adding the phase B into the phase A, uniformly stirring, and discharging to obtain the final product.
Test example I, patch test on human skin
The skin care liquids prepared in examples 3-4 and comparative examples 1-4 of the invention and the sunscreen cream prepared in example 5 are tested according to related requirements and determination methods of a human skin patch test in 2015 edition of cosmetic hygiene code issued by the ministry of health, skin reactions are observed at 0h, 6h, 12h, 24h and 48h respectively, the experimental results of all volunteer subjects are negative results, and no adverse skin reaction occurs. The skin care lotion or sunscreen cream provided by the invention is free of irritation and high in safety.
Test example two, stability test
(1) And (3) accelerated test: the skin moisturizers prepared in the embodiments 3-4 and the comparative examples 1-4 are taken for high-temperature and low-temperature storage stability test investigation, and the test conditions are as follows: 1) high temperature stability test: placing in an electric heating constant temperature incubator at 45 ℃ for 1 week, and observing after recovering the room temperature; 2) low-temperature stability test: the stability was observed after storage at 2 ℃ for 1 week, and the results are shown in Table 1 below.
Table 1 stability test results of skin lotions of each group
As can be seen from the above table 1, L-ascorbyl palmitate and laccaic acid have important influence on the stability of the skin care lotion, and the L-ascorbyl palmitate and the laccaic acid interact with each other to greatly improve the stability of the natural pigment ethosome in a water system and avoid the phenomenon of demulsification of the skin care lotion containing the natural pigment ethosome at high temperature and low temperature, so that chlorophyll and lycopene are separated out to form precipitates.
(2) And (3) normal-temperature long-term test: the skin moisturizers prepared in the embodiments 3-4 and the comparative examples 1-4 are taken for the test investigation of the storage stability at normal temperature, and the test conditions are as follows: the mixture is placed at the temperature of 23-28 ℃ and the relative humidity of 30-60%, and the stability is observed, and the results are shown in the following table 2.
Table 2 stability test results of skin lotions of each group in normal temperature long-term test
As shown in the table 2, L-ascorbyl palmitate and laccaic acid play an important role in the long-term stability of the skin moisturizer, and the L-ascorbyl palmitate and the laccaic acid interact with each other to greatly improve the storage stability of the skin moisturizer and prolong the service life of the skin moisturizer.
Test example III test of ultraviolet protective Properties
The skin moisturizers prepared in the embodiments 3-4 and the comparative examples 1-4 of the invention are respectively subjected to ultraviolet protection performance detection, specifically QB/T2410-1998 (ultraviolet absorbance method for evaluating sunscreen effect in UVB region of sunscreen cosmetic), the absorbance of the product at the wavelength of 280-400 nm is measured, the average values of the absorbance A at the wavelength of UVB and UVA regions are respectively obtained according to the following formula 1 and the formula 2 and are respectively recorded as AUVBAnd AUVAThe results are shown in Table 3.
UVB region 280-320 nm: AUVB＝(A280+A290+A300+A310+A320) /5 formula (1)
UVA region 330-400 nm: AUVA＝(A330+A340+A350+A360+A370+A380+A390+A400) /8 type (2)
Table 3 test results of ultraviolet protection effect of skin care liquids in each group
| Absorbance value | Example 3 | Example 4 | Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 |
| AUVB | 1.874 | 1.820 | 0.952 | 1.474 | 0.765 | 1.358 |
| AUVA | 1.758 | 1.716 | 0.840 | 1.322 | 0.547 | 1.146 |
Note: a is more than 0.5UVB/AUVA< 1.0: minimum ultraviolet protection; 1.1 < AUVB/AUVA< 1.5: medium ultraviolet protection; 1.6 < AUVB< 2.0: high-efficiency ultraviolet protection.
As can be seen from the above Table 3, the skin lotion provided by the present invention has the advantages of mutual coordination and interaction of the components, and high UV protection effect. The natural pigment ethosome in the comparative example 1 does not contain lycopene, and the ultraviolet protection effect is obviously reduced, which shows that the interaction of chlorophyll and lycopene can greatly improve the ultraviolet protection effect. The skin care lotion prepared by the comparison example 2 without lac acid and the comparison example 3 without L-ascorbyl palmitate and lac acid has obviously reduced ultraviolet protection effect, which shows that the deterioration of the stability of natural pigment ethosome has important influence on the ultraviolet protection effect of the skin care lotion. Comparative example 4 does not contain mountain rose callus cell suspension culture solution freeze-dried powder, and the ultraviolet protection effect is obviously reduced, which shows that the mountain rose callus cell suspension culture solution freeze-dried powder can improve the ultraviolet protection effect of the skin care lotion to a certain extent.
Test example four, antioxidation test
Free radicals are active chemical substances with extremely strong oxidizing capability, and a large number of free radicals generated in a body can also damage the skin and accelerate the formation of color spots and melanin. DPPH is a stable long-lived radical whose light absorption is diminished by pairing with its single electron when a radical scavenger is present, thereby evaluating the ability of a substance to scavenge free radicals and thus assess its antioxidant ability. The skin moisturizers prepared in example 3 and comparative examples 1-4 were tested for their DPPH radical scavenging rate by determining absorbance through DPPH radical neutralization test to evaluate the antioxidant effect of the skin moisturizers prepared in the present invention, and the results are shown in Table 4 below.
TABLE 4 DPPH radical scavenging ratio of skin lotions of each group
As can be seen from the above table 4, the skin moisturizer provided by the invention can effectively remove DPPH free radicals, and shows that the active skin moisturizer provided by the invention is reasonable in compounding and coordinated with each other, has a better antioxidant effect, and inhibits the formation of color spots and melanin.
Test example five post-basking repair Performance test
Screening 100 volunteer patients with different degrees of sunburn damage on the face, wherein symptoms of the sunburn damage are red swelling, peeling and dryness, the symptoms are randomly divided into 5 groups, 20 people in each group are respectively the group of example 3 and the group of comparative examples 1-4, the skin care liquids prepared in the example 3 and the group of comparative examples 1-4 are respectively used for each group, the skin care liquids are respectively used for 1 time in the morning and evening and are continuously used for 4 weeks, and the condition of the sunburn skin damage repair of each group of volunteer patients is evaluated, and the results are shown in the following tables 5 and 6.
TABLE 5 evaluation of skin improvement efficacy of skin lotions of each group
| Group of | Skin moisture content (%) | Skin roughness average Change (△ R3, μm) | Skin elasticity (Ur/Uf) |
| Example 3 | 49.38±5.28 | 40.57±4.62 | 0.94±0.12 |
| Comparative example 1 | 45.69±5.02 | 37.24±4.05 | 0.86±0.10 |
| Comparative example 2 | 42.74±4.85* | 34.80±3.75* | 0.74±0.09* |
| Comparative example 3 | 36.68±3.64** | 30.96±2.62** | 0.60±0.08** |
| Comparative example 4 | 42.02±5.79* | 33.75±4.26* | 0.70±0.12* |
Note: comparison with example 3, P*＜0.05、P**＜0.05。
TABLE 6 after-Sun repair Effect of various skin lotions
As can be seen from table 5 above, the skin lotion prepared in example 3 of the present invention can effectively improve the rough state of the skin, increase the moisture content of the skin, and make the skin elastic. As can be seen from table 6 above, the skin lotion prepared in example 3 of the present invention can promote the repair of post-sunburn wounds, and has a certain therapeutic effect on post-sunburn discomfort symptoms such as dryness, peeling and redness and swelling.
The above is only a preferred embodiment of the present invention, and it should be noted that the above preferred embodiment should not be considered as limiting the present invention, and the protection scope of the present invention should be subject to the scope defined by the claims. It will be apparent to those skilled in the art that various modifications and adaptations can be made without departing from the spirit and scope of the invention, and these modifications and adaptations should be considered within the scope of the invention.
Claims (9)
1. The active skin care composition is characterized by comprising the following components in parts by weight:
1-10 parts of natural pigment ethosome;
0.05-4 parts of glycyrrhetinic acid;
0.05-2 parts of palmatine;
0.1-1 part of cysteinyl glycine;
0.1-1 part of L-ascorbyl palmitate;
1-10 parts of mountain rose callus cell suspension culture solution freeze-dried powder.
2. The active skin care composition according to claim 1, further comprising 0.01 to 1 part of laccaic acid.
3. The active skin care composition according to claim 1, wherein the skin care composition comprises the following components in parts by weight:
5-8 parts of natural pigment ethosome;
1-2 parts of glycyrrhetinic acid;
0.1-1 part of palmatine;
0.5-1 part of cysteinylglycine;
0.5-1 part of L-ascorbyl palmitate;
0.1-0.5 part of laccaic acid;
6-10 parts of mountain rose callus cell suspension culture solution freeze-dried powder.
4. The active skin care composition according to any of claims 1-3, wherein the natural pigment ethosome comprises the following components in parts by weight: 0.1-1 part of natural pigment, 4-12 parts of soybean lecithin, 20-30 parts of ethanol, 1-2 parts of polyethylene glycol stearate, 3-5 parts of hydroxyethyl piperazine ethanesulfonic acid and 20-30 parts of deionized water.
5. The active skin care composition according to claim 4, wherein the natural pigment is composed of chlorophyll and lycopene in a mass ratio of 1: 0.1-1.
6. The active skin care composition according to claim 5, wherein the natural pigment consists of chlorophyll and lycopene in a mass ratio of 1: 0.6.
7. The active skin care composition of claim 5, wherein the preparation of the natural pigment ethosome comprises the steps of:
weighing soybean lecithin, dissolving with ethanol, adding polyethylene glycol stearate and natural pigment, and mixing; at 400r.min-1Under the condition of (1), using a peristaltic pump at 100 mu L.min-1The pre-dissolved water solution of the hydroxyethyl piperazine ethanesulfonic acid is injected into the ethanol solution at the speed of 10000r.min-1Stirring for 2min to homogenize, treating with 200nm microporous homogeneous membrane to obtain alcoholic body fluid, cooling and solidifying to obtain natural pigment ethosome.
8. The active skin care composition according to claim 3, wherein the freeze-dried powder of mountain rose callus cell suspension culture solution is prepared by the following steps:
(1) inoculating sterile mountain rose leaves into a solid culture medium of MS + 0.8-1.2 mg/L6-BA + 1.2-2.0 mg/L2, 4-D +4g/L agar +25g/L sucrose to induce callus, wherein the subculture time is 105-120 days, and selecting loose pale yellow white callus which is subcultured for 3 times continuously;
(2) taking the loose pale yellow white callus obtained in the step (1), cutting the callus into mung bean size by using sterilized forceps, inoculating the mung bean size into a 100ml conical flask containing 40ml of 1/2MS + 1.5-2.0 mg/L6-BA + 0.5-1.0 mg/L2, 4-D +30g/L sucrose and a liquid culture medium with pH of 5.8, carrying out suspension culture on the conical flask at the constant temperature of 25 ℃ on a shaking bed at 100rpm under 12h/D illumination time, after culturing for 15D, filtering by using a 50-mesh cell sieve, taking filtrate, filtering by using an 80-mesh cell sieve, taking the filtrate, sterilizing and freeze-drying to obtain the callus.
9. Use of an active skin care composition according to any one of claims 1 to 8 for the preparation of a skin care product or cosmetic with sunscreen repair, skin aging retardation, whitening and spot removal.
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| JP2003012437A (en) * | 2001-07-02 | 2003-01-15 | Nisshin Oillio Group Ltd | Film-forming agent, skin care agent and cosmetic composition |
| CN106214507A (en) * | 2016-08-31 | 2016-12-14 | 广东科玮生物技术股份有限公司 | A kind of chlorophyll sunlight screening skin-protecting product and preparation method thereof |
| CN107227289A (en) * | 2017-05-08 | 2017-10-03 | 广州赛莱拉干细胞科技股份有限公司 | A kind of camellia cell extract and its extracting method and application |
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