CN110664671B - Plant extract composition and application thereof in sunscreen, after-sun repair, photoaging prevention and whitening cosmetics - Google Patents
Plant extract composition and application thereof in sunscreen, after-sun repair, photoaging prevention and whitening cosmetics Download PDFInfo
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
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- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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Abstract
The invention discloses an application of a natural plant rosemary, honeysuckle and ligusticum wallichii extract composition in cosmetics for sun protection, after-sun repair, inflammation resistance, photoaging resistance and whitening, and belongs to the field of traditional Chinese medicines and cosmetics. The invention applies rosemary, honeysuckle and ligusticum wallichii extract to cosmetics, verifies that the combination of two or three components can effectively resist the damage of ultraviolet rays to the skin, and has obvious effect on repairing skin cells after the ultraviolet rays are damaged. The plant extract composition with the sun-screening, after-sun repairing, anti-inflammatory, anti-photoaging and whitening effects can be used as an effective active ingredient to be prepared into various formulations such as cream, spray, powder and the like, and is used for cosmetics.
Description
Technical Field
The invention belongs to the field of traditional Chinese medicines and cosmetics, and relates to a natural plant extract composition containing rosemary extract, honeysuckle extract and/or ligusticum wallichii composition and application thereof in sunscreen, after-sun repair, anti-inflammation, photoaging prevention and whitening cosmetics.
Background
In recent years, the increase of natural environmental pollution and atmospheric destruction has led to the increase of ultraviolet rays irradiated to the ground, and the number of patients with skin aging, solar dermatitis and even skin cancer has significantly increased, so that the search for safe, effective and stable cosmetic sunscreens and after-sun repair agents has been the focus of much research and development.
Among the three types of ultraviolet rays having different wavelength ranges, two portions of ultraviolet rays can be irradiated to the ground, i.e., the long-wavelength ultraviolet ray UVA having a wavelength of 320-. UVA has extremely strong penetrating power, and can reach the deep part of the dermis layer through the surface layer of the skin to cause premature aging of the skin; UVB mainly acts on the cell surface to cause skin tanning, and can cause erythema generation and peeling for a long time, even damage cell DNA and cause skin cancer. Naturally, UVA and UVB have a synergistic effect on skin damage, and UVA can significantly aggravate acute damage caused by UVB and increase carcinogenic potential, so daily protection against ultraviolet rays is very important.
The number of sunscreen cosmetics on the market is large, and the sunscreen capacity is mainly determined by sunscreen agents. Titanium dioxide, zinc oxide and other physical sun-screening agents can effectively shield the skin from being damaged by ultraviolet rays, but when the using amount is too large or the using time is long, the air permeability is poor, and pores are easy to block; chemical sunscreens such as benzophenone derivatives are also widely used, but they are likely to cause skin allergy, photolysis, and the like, and therefore, when they are used for a long period of time, the intermediate products generated are likely to cause damage to the skin.
Nowadays, the improvement of living standard leads people to continuously improve the requirements of cosmetics, the situation of skin care of the public is also more and more rational, the safety and effectiveness of the added components are more and more emphasized, and the cosmetics which are natural, stable and remarkable in sunscreen effect are favored. Research for many years shows that some traditional Chinese medicines can play an effective role in preventing and treating skin photodamage and photoaging, and multiple researches show that skin aging is closely related to improper sun protection, so that daily sun protection is very important, and the traditional Chinese medicines can remove oxygen free radicals, repair collagen fibers, improve microcirculation and the like, and the rhizoma kaempferiae extract, the curcuma longa rhizome extract, the aloe extract and the like have good sun protection effects. The sunscreen component extracted from the plant has stable drug property and lasting drug efficacy, has no stimulation to skin, is safe and effective, has wide development prospect, and is one of the market development directions of cosmetics.
Disclosure of Invention
The invention aims to provide a safe and effective natural plant extract composition which has the effects of sun-screening, after-sun repairing, anti-inflammation, anti-photoaging and whitening and moisturizing effects on skin cells.
The invention provides application of rosemary extract, honeysuckle extract, ligusticum wallichii extract and a composition thereof in sunscreen, after-sun repair, anti-inflammation, anti-photoaging, whitening, moisturizing or anti-aging of cosmetics.
The invention provides a plant extract composition with sun-screening, after-sun repairing and whitening, anti-inflammatory, anti-photoaging, moisturizing or anti-aging effects, wherein any two of rosemary extract, honeysuckle extract or ligusticum wallichii extract are used as active ingredients of the plant extract composition, and the mass part ratio of the two active ingredients is (1-9): (1-9); or the plant extract composition contains rosemary extract, honeysuckle extract and ligusticum wallichii extract as active ingredients, and the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract are mixed according to the mass part ratio of (1-9): (1-9): (1-9).
In the invention, any two of rosemary extract, honeysuckle extract or ligusticum wallichii extract are taken as active ingredients of the plant extract composition, and the plant extract composition has a remarkable synergistic enhancement effect between any two of the rosemary extract, the honeysuckle extract or the ligusticum wallichii extract within the mass ratio range. From fig. 2 and fig. 5, it can be seen that the effect of the combination of rosemary extract and honeysuckle extract is better than that of the combination of rosemary extract and honeysuckle extract when the combination is used alone, and the combination has good anti-ultraviolet injury effect under different concentration ratios; similarly, as shown in fig. 3 and fig. 6, the effect of the combination of rosemary extract and chuanxiong rhizome extract is better than that of the combination of rosemary extract and chuanxiong rhizome extract when the combination is used alone, and the combination has good anti-ultraviolet injury effect under different concentration ratios; as shown in FIG. 4 and FIG. 7, the effect of the combination of the honeysuckle extract and the chuanxiong rhizome extract is better than that of the combination of the honeysuckle extract and the chuanxiong rhizome extract when the combination is used alone, and the honeysuckle extract and the chuanxiong rhizome extract have good ultraviolet injury resistance under different concentration ratios. Preferably, the mass part ratio of any two active ingredients is (4-6): (4-6); more preferably, the mass part ratio of any two active ingredients is 1: 1.
in the invention, the plant extract composition takes rosemary extract, honeysuckle extract and ligusticum wallichii extract as active ingredients, and preferably, the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract are in a mass part ratio of (4-6): (4-6): (4-6); more preferably, the mass part ratio of the rosemary extract to the honeysuckle extract to the ligusticum wallichii extract is 1: 1: 1. within the mass ratio range, the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract have a remarkable synergistic enhancement effect, namely as shown in fig. 8 and 9, compared with a single extract, the composition of the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract has better protection and repair effects on ultraviolet irradiation.
The invention also provides a cosmetic composition containing the extract composition. Wherein the active component of each extract (rosemary extract, honeysuckle extract or ligusticum wallichii extract) accounts for 0.05-3% of the mass percentage of the cosmetic matrix, and the balance is the cosmetic matrix.
Preferably, the active ingredients of the composition (rosemary extract, honeysuckle extract or ligusticum wallichii extract) account for 3% of the mass of the cosmetic matrix, and the balance is the cosmetic matrix.
In the present invention, the cosmetic base may be, but is not limited to, a base mixture (weight percentage) consisting of:
6% glycerol, 5% mineral oil, 4% isopropyl palmitate, 3.5% caprylic/capric triglyceride, 2% steareth-2, 2% steareth-21, 1.5% cetearyl alcohol, 1% polyacrylate-13/polyisobutylene/polysorbate-20, 0.8% glyceryl stearate, 0.2% allantoin, 0.2% methylparaben, 0.15% bis (hydroxymethyl) imidazolidinyl urea, 0.1% propylparaben, 0.05% disodium EDTA, and the balance water. Or the like, or, alternatively,
6% cetearyl ethyl hexanoate, 5% glycerol, 4% ethyl oleate, ethyl stearate, shea butter unsaponifiable matter, ethyl linoleate, 3% caprylic capric triglyceride, 3% methyl propylene glycol, 3% arachidyl alcohol, behenyl alcohol, arachidyl alcohol glucoside, water, glucose, 2.5% cetearyl alcohol, 2% polydimethylsiloxane, 1% C14-22 alcohol, C12-20 alkyl glucoside, water, glucose, 0.6% phenoxyethanol, 0.5% polyacrylate crosspolymer-6, water, tert-butanol, 0.2% ethylhexyl glycerol, 0.2% hexylene glycol/caprylyl glycol, 0.2% gum arabic, xanthan gum, and the balance water.
The cream prepared by the method is uniform in coating, fine in hand feeling, easy to absorb, light in natural herbal fragrance, and fresh and mild.
The invention also provides application of the plant extract composition or the cosmetic composition in preparing products for resisting damage of ultraviolet rays to skin and repairing damaged skin.
The invention also provides application of the plant extract composition or the cosmetic composition in preparing cosmetics with sun-screening, after-sun repairing, anti-inflammatory, anti-photoaging, whitening, moisturizing or anti-aging effects.
The invention also provides application of the plant extract composition or the cosmetic composition in preparing sunscreen cosmetics or freckle-removing cosmetics.
In the invention, the cosmetic can be various cosmetics such as cream, emulsion, facial mask, toner, skin lotion, ointment, oil solution, gel, aerosol, solution, or film coating agent.
The invention verifies the effects of the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract on the aspects of ultraviolet resistance, sun protection, after-sun repair, inflammation resistance, light aging resistance, whitening and moisture retention of skin, and verifies the synergistic effect of the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract on the aspects of sun protection, after-sun repair, inflammation resistance, light aging resistance and whitening for the first time through in vitro and in vivo experiments. The experimental result shows that the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract can effectively enhance the ultraviolet ray resistance of the skin, have obvious repairing effect on cells damaged by ultraviolet rays, and can also obviously reduce the inflammatory reaction of the skin and the formation of melanin. The raw materials of rosemary extract, honeysuckle extract and ligusticum wallichii extract are natural and green, are non-toxic and non-irritant, and have the advantages of simple process, low production cost, mild effect on skin, safety and effectiveness.
In the present invention, the whitening refers to preventing the deposition of melanin in the skin, or reducing the production of melanin in skin cells, or degrading already deposited melanin.
In the invention, the anti-inflammation refers to the application of the product for preventing, reducing and eliminating skin inflammatory reaction. Wherein the inflammation refers to skin inflammation reaction caused by ultraviolet irradiation, and can reduce the production of various intracellular inflammatory factors.
In the present invention, the moisturizing means maintaining the skin moist, keeping the skin moist and elastic, or making the dry skin moist and elastic.
The invention has the beneficial effects that the invention provides the application of the composition containing the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract in the preparation of cosmetics for preventing sun, repairing after sun, resisting inflammation, preventing photoaging and whitening. The invention applies rosemary extract, honeysuckle extract and ligusticum wallichii extract to cosmetics, firstly provides that the composition of any two active components has the effects of sun screening, after-sun repair, inflammation resistance, photoaging resistance, whitening and the like, and further provides that the composition formed by combining the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract can greatly improve the sun screening effect and the after-sun cell repair effect, is safe and stable, keeps skin moist, whitens the skin, prevents photoaging and resists skin aging. The invention can be used as an effective active ingredient to be prepared into various formulations such as cream, spray, powder and the like, and is widely applied to cosmetics, skin care products and the like.
Drawings
FIG. 1 is a graph showing the effect of rosemary extract, honeysuckle extract and chuanxiong rhizome extract on cell activity, and FIGS. 1-1 show the effect of rosemary extract at different concentrations on cell activity; FIGS. 1-2 the effect of different concentrations of honeysuckle extract on cell viability; FIGS. 1-3 the effect of different concentrations of Chuan Xiong extract on cell activity; p <0.05 compared to control group.
FIG. 2 is a graph comparing the protective effect of the composition on HaCaT cells by UV irradiation with rosemary extract and honeysuckle extract, p, compared with the UV control<0.05，**p<0.01，***p<0.001; compared with the composition group,##p<0.01，###p<0.001。
FIG. 3 is a graph comparing the protective effect of UV irradiation HaCaT cells of the composition with rosemary extract and Chuan Xiong extract, and UV control<0.01，***p<0.001; compared with the composition group,#p<0.05，##p<0.01，###p<0.001。
FIG. 4 is a graph comparing the protective effect of the composition on HaCaT cells by UV irradiation with flos Lonicerae extract and rhizoma Ligustici Chuanxiong extract, and p is compared with ultraviolet control group<0.05，**p<0.01，***p<0.001; compared with the composition group,#p<0.05，###p<0.001。
fig. 5 is a graph showing the results of the uv protection experiment with rosemary extract and honeysuckle extract at different concentration ratios, compared to the uv control group, p <0.01 and p < 0.001.
Fig. 6 is a graph showing the results of the uv protection experiment with rosemary extract and chuanxiong rhizome extract at two different concentration ratios, p <0.01 and p <0.001, compared with the uv control group.
Fig. 7 is a graph showing the results of the protection experiment of uv light by using two different concentration ratios of the honeysuckle extract and the chuanxiong rhizome extract, wherein p is less than 0.01 and p is less than 0.001 compared with the uv control group.
FIG. 8 is a graph comparing the protective effect of the composition on HaCaT cells exposed to UV irradiation with Rosmarinus officinalis extract, Lonicera Japonica flos extract, and rhizoma Ligustici Chuanxiong extract, and comparing with UV control group<0.01，***p<0.001, compared to the composition group,#p<0.05，##p<0.01，###P<0.001。
FIG. 9 is a graph comparing the effect of the composition on repairing HaCaT cell damage by UV irradiation with rosemary extract, honeysuckle extract, and Chuan Xiong extract, and comparing with the UV control,*P<0.05，**p<0.01，***p<0.001, compared with composition group，##p<0.01，###p<0.001。
FIG. 10 is a graph comparing the anti-inflammatory effect of the composition on UV-irradiated HaCaT cells with rosemary extract, honeysuckle extract, and Chuan Xiong extract, and FIG. 10-1 is a graph comparing the effect of the composition on UV-irradiated HaCaT cells IL-1 β with rosemary extract, honeysuckle extract, and Chuan Xiong extract, and compared with the UV control group<0.01，***p<0.001, compared to the composition group,###p<0.001; FIG. 10-2 is a graph comparing the effect of the composition on UV irradiation of HaCaT cell IL-6 with rosemary extract, honeysuckle extract, and chuanxiong rhizome extract, and on UV irradiation of HaCaT cell IL-6 with UV control<0.05，**p<0.01，***p<0.001, compared to the composition group,#p<0.05，##p<0.01。
FIG. 11 is a graph comparing the inhibitory effect of the composition on melanin content in B16F10 cells, and rosemary extract, honeysuckle extract, and chuanxiong rhizome extract, with control group<0.01，***p<0.001, compared to the composition group,#p<0.05，##p<0.01。
Detailed Description
The present invention will be described in further detail with reference to the following specific examples and the accompanying drawings. The procedures, conditions, experimental methods and the like for carrying out the present invention are general knowledge and common general knowledge in the art, except for those specifically mentioned below, and the present invention is not particularly limited.
The rosemary extract is a cosmetic raw material and can be purchased from the market. Specific said rosemary extracts may include, but are not limited to, rosemary flower/leaf/stem extracts, rosemary flower extracts, rosemary leaf powders, rosemary leaf waters, rosemary leaf extracts, and rosemary leaf oils; preferably, it is rosemary leaf powder, rosemary flower/leaf/stem extract, rosemary flower extract, rosemary extract or rosemary leaf extract.
The honeysuckle extract (also called honeysuckle extract) is a cosmetic raw material and can be purchased from the market. Specific honeysuckle extracts may include, but are not limited to, honeysuckle flower extract, honeysuckle leaf extract, and honeysuckle stem extract.
The ligusticum wallichii extract is a cosmetic raw material and can be purchased from the market. The cnidium officinale Makino extract may include, but is not limited to, cnidium officinale Makino root powder, cnidium officinale Makino root extract, cnidium officinale Makino extract, and cnidium officinale Makino oil.
The composition can be prepared into various dosage forms such as cream, spray, powder and the like, and can be used as an active ingredient for cosmetics. The obtained cosmetic can be in the form of cream, emulsion, facial mask, astringent, skin caring lotion, ointment, oil, gel, aerosol, solution, or plastics.
Example 1 Effect of Rosmarinus officinalis extract, flos Lonicerae extract, and rhizoma Ligustici Chuanxiong extract on cell activity.
The human immortalized epidermal cell HaCaT cell is divided into 1 × 105The density of each well is inoculated into a 96-well plate, after the cells adhere to the wall, the original cell culture medium is changed into 1640 culture medium containing medicines, and the concentrations of the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract in the experimental groups are respectively (0mg/mL, 0.25mg/mL, 0.5mg/mL, 0.75mg/mL, 1mg/mL, 1.5mg/mL and 2 mg/mL). Adding CCK-8 after 24h to detect the proliferation activity of the cells, wherein 10 mu l of CCK-8 solution is added into each hole, and the color of the culture medium turns orange after incubation for 1h in an incubator at 37 ℃; the specific incubation time depends on different cells, and the absorbance value at the wavelength of 450nm is detected, wherein the higher the absorbance value is, the higher the proliferation activity of the cells is, and the lower the proliferation activity of the cells is. As shown in FIGS. 1-1, 1-2, and 1-3, when the concentration of rosemary extract is less than or equal to 1.5mg/mL, the concentration of honeysuckle extract is less than or equal to 1.5mg/mL, and the concentration of chuanxiong rhizome extract is less than or equal to 1.5mg/mL, no toxic effect is generated on the activity of HaCaT cells, and the following experiments are conducted with the drug concentration of 0.5mg/mL or 0.75mg/mL, as compared with the control group without drug.
Example 2 comparison of the protective effect of the composition on HaCaT cells by UV irradiation with Rosemary extract and honeysuckle extract.
The human immortalized epidermal cell HaCaT cell is divided into 1 × 105Density inoculation of each wellIn a 96-well plate, after the cells are attached to the wall, the original cell culture medium is changed into 1640 culture medium containing medicines, the experiment is divided into the following groups: control group, ultraviolet group, composition group (containing 0.25mg/mL rosemary extract and 0.25mg/mL honeysuckle extract), rosemary extract group (0.25mg/mL, 0.5mg/mL), and honeysuckle extract group (0.25mg/mL, 0.5 mg/mL). After 24h drug incubation, the drug medium was replaced with PBS and irradiated with a cell sun UV simulator (purchased from France) at a dose of 50mJ/cm2UVB+550 mJ/cm2UVA, where experimental control cells were neither dosed nor uv-irradiated. After the UV irradiation is finished, PBS is replaced by 1640 culture medium containing extracts of each group, and CO is added2The incubator continues to culture for 24 h. And finally, adding CCK8 to detect the cell activity, and detecting the light absorption value of the cell supernatant by using an enzyme-labeling instrument. The experiment was repeated three times and the results are shown statistically in figure 2. The activity of HaCaT cells after ultraviolet irradiation is obviously reduced, the cell activity is enhanced after the composition, the rosemary and the honeysuckle extract are respectively added, compared with a UV control group, the difference is obvious, and the statistical significance is achieved<0.05，**p<0.01，***p<0.001, compared with the rosemary extract group and the honeysuckle extract group, the cell activity of the composition group is obviously increased, the difference has statistical significance,##p<0.01，###p<0.001. the combination of the rosemary extract and the honeysuckle extract can obviously reduce the damage of ultraviolet rays to cells, and the combined effect is obviously superior to the protection effect of the single extract, and has obvious synergistic effect.
Example 3 comparison of the protective effect of the composition on HaCaT cells by UV irradiation with Rosemary extract and Ligusticum wallichii extract.
Implementation procedures referring to example 2, experimental groups: control group, ultraviolet group, composition group (containing 0.25mg/mL rosemary extract and 0.25mg/mL rhizoma Ligustici Chuanxiong extract), rosemary extract group (0.25mg/mL, 0.5mg/mL), and rhizoma Ligustici Chuanxiong extract group (0.25mg/mL, 0.5 mg/mL). The statistics of the results are shown in FIG. 3. The activity of HaCaT cells after ultraviolet irradiation is obviously reduced and a semi-lethal phenomenon appears, after the composition, the rosemary and the ligusticum wallichii extract are respectively added, the cell activity is enhanced, compared with a UV control group, the difference is obvious, the statistical significance is achieved, p is less than 0.01, p is less than 0.001, compared with a rosemary extract group and a ligusticum wallichii extract group, the cell activity of the composition group is obviously increased, the difference has the statistical significance, # p is less than 0.05, # p is less than 0.01, and # p is less than 0.001. The combination of the rosemary extract and the ligusticum wallichii extract can obviously reduce the damage of ultraviolet rays to cells, and the combined effect is obviously superior to the protection effect of the single extract, so that the rosemary extract and the ligusticum wallichii extract have obvious synergistic effect.
Example 4 comparison of the protective effect of the composition on HaCaT cells by UV irradiation with honeysuckle extract and chuanxiong rhizome extract.
Implementation procedures referring to example 2, experimental groups: control group, ultraviolet group, composition group (containing 0.25mg/mL flos Lonicerae extract and 0.25mg/mL rhizoma Ligustici Chuanxiong extract), flos Lonicerae extract group (0.25mg/mL, 0.5mg/mL), and rhizoma Ligustici Chuanxiong extract group (0.25mg/mL, 0.5 mg/mL). The statistics of the results are shown in FIG. 4. The activity of HaCaT cells after ultraviolet irradiation is obviously reduced and a semi-lethal phenomenon appears, after the composition, the honeysuckle and the ligusticum wallichii extract are respectively added, the cell activity is enhanced, compared with a UV control group, the difference is obvious, the statistical significance is achieved, and p<0.05，**p<0.01，***p<0.001, compared with the honeysuckle extract group and the ligusticum wallichii extract group, the cell activity of the composition group is obviously increased, the difference has statistical significance,#p<0.05，###p<0.001. the combination of the honeysuckle extract and the ligusticum wallichii extract can obviously reduce the damage of ultraviolet rays to cells, and the combined effect is obviously better than the protection effect of the single extract, so that the honeysuckle extract and the ligusticum wallichii extract have obvious synergistic effect.
Example 5. test for protection of ultraviolet light by rosemary extract and honeysuckle extract at different concentration ratios.
Example 2, experimental groups were divided into a control group, an ultraviolet group, and an extract composition group (consisting of rosemary and honeysuckle extracts at different ratios), wherein the composition groups were 9, and the concentrations of rosemary extract 0.5mg/mL and honeysuckle extract 0.5mg/mL were regarded as percent, and the composition mass ratios were 1:9 (i.e., 0.05mg/mL rosemary extract and 0.45mg/mL honeysuckle extract, labeled as compositions 1-1), 2:8 (i.e., 0.1mg/mL rosemary extract and 0.4mg/mL honeysuckle extract, labeled as compositions 1-2), 3:7 (i.e., 0.15mg/mL rosemary extract and 0.35mg/mL honeysuckle extract, labeled as compositions 1-3), 4:6 (i.e., 0.2mg/mL rosemary extract and 0.3mg/mL honeysuckle extract, labeled as compositions 1-4), 5:5 (i.e., 0.25mg/mL rosemary extract and 0.25mg/mL honeysuckle extract, labeled as compositions 1-5), 6:4 (i.e., 0.3mg/mL rosemary extract and 0.2mg/mL honeysuckle extract, labeled as compositions 1-6), 7:3 (i.e., 0.35mg/mL rosemary extract and 0.15mg/mL honeysuckle extract, labeled as compositions 1-7), 8:2 (i.e., 0.4mg/mL rosemary extract and 0.1mg/mL honeysuckle extract, labeled as compositions 1-8), 9:1 (i.e., 0.45mg/mL rosemary extract and 0.05mg/mL honeysuckle extract, labeled as compositions 1-9). The results are shown in fig. 5, the HaCaT cell activity after UV irradiation was significantly reduced, a semi-lethal phenomenon was observed, and the cell activity was enhanced after addition of each composition, which was significantly different from the UV control group, and was statistically significant, # p <0.01, and # p < 0.001. The rosemary extract and the honeysuckle extract have better cell protection effect within the mass part ratio range of 1-9: 1-9; wherein the synergistic effect is more obvious within the mass part ratio range of 2-7: 3-8; more preferably, the mass part ratio is 4-6: 4-6; wherein the optimal mass part ratio is 1:1(5: 5).
Example 6 protection experiment of UV light by rosemary extract and Chuan Xiong extract at two different concentration ratios.
Referring to example 5, the experimental groups were divided into a control group, an ultraviolet group, and an extract composition group (composed of rosemary and chuanxiong rhizome extracts at different ratios), wherein the composition group was 9, the concentrations of rosemary extract 0.5mg/mL and chuanxiong rhizome extract 0.5mg/mL were respectively regarded as percent, and the composition mass ratios were 1:9 (i.e., rosemary extract 0.05mg/mL and chuanxiong rhizome extract 0.45mg/mL, and labeled as compositions 2-1), 2:8 (i.e., rosemary extract 0.1mg/mL and chuanxiong rhizome extract 0.4mg/mL, and labeled as compositions 2-2), 3:7 (i.e., rosemary extract 0.15mg/mL and chuanxiong rhizome extract 0.35mg/mL, and labeled as compositions 2-3), 4:6 (i.e., rosemary extract 0.2mg/mL and chuanxiong rhizome extract 0.3mg/mL, labeled as composition 2-4), 5:5 (i.e., 0.25mg/mL rosemary extract and 0.25mg/mL chuanxiong rhizome extract, labeled as composition 2-5), 6:4 (i.e., 0.3mg/mL rosemary extract and 0.2mg/mL chuanxiong rhizome extract, labeled as composition 2-6), 7:3 (i.e., 0.35mg/mL rosemary extract and 0.15mg/mL chuanxiong rhizome extract, labeled as composition 2-7), 8:2 (i.e., 0.4mg/mL rosemary extract and 0.1mg/mL chuanxiong rhizome extract, labeled as composition 2-8), 9:1 (i.e., 0.45mg/mL rosemary extract and 0.05mg/mL chuanxiong rhizome extract, labeled as composition 2-9). The results are shown in fig. 6, the HaCaT cell activity after UV irradiation was significantly reduced, a semi-lethal phenomenon was observed, and the cell activity was enhanced after addition of each composition group, which was significantly different from the UV control group, and was statistically significant, # p <0.01, # p < 0.001.
The rosemary extract and the ligusticum wallichii extract have better cell protection effect within the mass part ratio range of 1-9: 1-9; wherein the synergistic effect is more obvious within the mass part ratio range of 1-6: 4-9; more preferably, the mass part ratio is 4-6: 4-6; wherein the optimal mass part ratio is 1:1(5: 5).
Example 7. experiments on the protection of ultraviolet light by two different concentration ratios of honeysuckle extract and chuanxiong rhizome extract.
Referring to example 5, the experimental groups were divided into a control group, an ultraviolet group, and an extract composition group (honeysuckle and ligusticum wallichii extract at different ratios), the composition group was 9, the concentrations of 0.5mg/mL of honeysuckle extract and 0.5mg/mL of ligusticum wallichii extract were regarded as percent, the composition mass ratios were 1:9 (i.e. 0.05mg/mL of honeysuckle extract and 0.45mg/mL of ligusticum wallichii extract, respectively, and labeled as composition 3-1), 2:8 (i.e. 0.1mg/mL of honeysuckle extract and 0.4mg/mL of ligusticum wallichii extract, labeled as composition 3-2), 3:7 (i.e. 0.15mg/mL of honeysuckle extract and 0.35mg/mL of ligusticum wallichii extract, labeled as composition 3-3), 4:6 (i.e. 0.2mg/mL of honeysuckle extract and 0.3mg/mL of ligusticum wallichii extract, labeled as composition 3-4), 5:5 (i.e., 0.25mg/mL of the honeysuckle extract and 0.25mg/mL of the Ligusticum wallichii extract, labeled as composition 3-5), 6:4 (i.e., 0.3mg/mL of the honeysuckle extract and 0.2mg/mL of the Ligusticum wallichii extract, labeled as composition 3-6), 7:3 (i.e., 0.35mg/mL of the honeysuckle extract and 0.15mg/mL of the Ligusticum wallichii extract, labeled as composition 3-7), 8:2 (i.e., 0.4mg/mL of the honeysuckle extract and 0.1mg/mL of the Ligusticum wallichii extract, labeled as composition 3-8), 9:1 (i.e., 0.45mg/mL of the honeysuckle extract and 0.05mg/mL of the Ligusticum wallichii extract, labeled as composition 3-9). The results are shown in fig. 7, the HaCaT cell activity after UV irradiation was significantly reduced, a semi-lethal phenomenon was observed, and the cell activity was enhanced after addition of each composition group, which was significantly different from the UV control group, and was statistically significant, # p <0.01, # p < 0.001.
The honeysuckle extract and the ligusticum wallichii extract have better cell protection effect within the mass part ratio range of 1-9: 1-9; wherein the synergistic effect is more obvious within the mass part ratio range of 1-6: 4-9; more preferably, the mass part ratio is 4-6: 4-6; wherein the optimal mass part ratio is 1:1(5: 5).
Example 8 comparison of the protective effect of the composition on HaCaT cells by UV irradiation of ultraviolet light with Rosemary extract, honeysuckle extract, Ligusticum wallichii extract.
Implementation procedures referring to example 2, experimental groups: control group, ultraviolet group, composition group (containing 0.25mg/mL rosemary extract, 0.25mg/mL honeysuckle extract and 0.25mg/mL chuanxiong rhizome extract), rosemary extract group (0.75mg/mL), honeysuckle extract group (0.75mg/mL), and chuanxiong rhizome extract group (0.75 mg/mL). The experiment was repeated three times and the statistics are shown in figure 8. The activity of HaCaT cells after ultraviolet irradiation is obviously reduced and a semi-lethal phenomenon appears, after the composition, the rosemary, the honeysuckle and the ligusticum wallichii extract are added, the cell activity is enhanced, compared with a UV control group, the difference is obvious, and the statistical significance is achieved<0.01，***p<0.001, compared with rosemary extract group, honeysuckle extract group and ligusticum wallichii extract group, the cell activity of the composition group is obviously increased, the difference has statistical significance,#p<0.05，##p<0.01，###p<0.001. the combination of the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract can obviously reduce the damage of ultraviolet rays to cells, and the combined effect is obviously better than the protection effect of the single extract, so that the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract have obvious protection effectAnd (4) synergistic effect.
Example 9 comparison of the repair of HaCaT cell damage by UV irradiation of the composition with Rosemary extract, honeysuckle extract, Ligusticum wallichii extract.
The human immortalized epidermal cell HaCaT cell is divided into 1 × 105The density of each well was inoculated into a 96-well plate, and after 24 hours of cell culture, the medium was replaced with PBS and cell irradiation was performed with a sunlight UV simulator (purchased from France) at a dose of 50mJ/cm2UVB+550mJ/cm2UVA, wherein the cells of the experimental control group are not irradiated with ultraviolet light. After the cells were cultured for 12 hours after UV irradiation, the UV-irradiated cells were grouped as follows: ultraviolet control group, composition group (containing 0.25mg/mL rosemary extract, 0.25mg/mL honeysuckle extract and 0.25mg/mL chuanxiong rhizome extract), rosemary extract group (0.75mg/mL), honeysuckle extract group (0.75mg/mL), and chuanxiong rhizome extract group (0.75 mg/mL). Then replacing normal culture medium for the normal cell control group not irradiated with ultraviolet and the cell control group irradiated with ultraviolet, replacing culture medium containing medicine for other groups, and continuing to use CO2Culturing in an incubator for 24 h. And finally, adding CCK8 to detect the cell activity, and detecting the light absorption value of the cell supernatant by using an enzyme-labeling instrument. The experiment was repeated three times and the statistics are shown in figure 9. The HaCaT cell activity of the ultraviolet control group is obviously reduced, the cell activity is obviously recovered after the composition, the rosemary, the honeysuckle and the ligusticum wallichii extract are added, compared with the ultraviolet control group, the difference is obvious, the statistical significance is achieved, and p<0.01，**p<0.01, compared with rosemary extract group, honeysuckle extract group and ligusticum wallichii extract group, the cell activity is increased more obviously, which has statistical significance,#p<0.05，##p<0.01. the combination of the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract can obviously improve the capability of repairing the cell activity after the cell is damaged by ultraviolet irradiation, and the effect of promoting the repair after the combination is obviously better than that of a single extract, so that the synergistic effect is obvious.
Example 10 composition is compared to rosemary extract, honeysuckle extract, ligusticum wallichii extract for their anti-inflammatory effect on HaCaT cells irradiated with UV.
Human immortalized epidermal cell HaCaT cell 2X 105The cells are inoculated into a 24-well plate, cultured for 24 hours and irradiated with ultraviolet light, the specific treatment method is shown in example 9, and the experimental grouping is as follows: control group (without UV), ultraviolet group, composition group, herba Rosmarini officinalis extract group, flos Lonicerae extract group, and rhizoma Ligustici Chuanxiong extract group, each group is provided with 4 multiple holes. After the experiment is finished, each group of cells is collected respectively, and RNA is extracted. The expression conditions of the inflammatory factors IL-1 beta and IL-6 in HaCaT cells are detected in a fluorescent real-time quantitative PCR instrument, and the experimental results are shown in figures 10-1 and 10-2. The expression level of inflammatory factors of HaCaT cells in the ultraviolet control group is obviously increased, the inflammatory factors of the cells are obviously reduced after the composition, the rosemary, the honeysuckle and the ligusticum wallichii extract are added, and compared with the ultraviolet control group, the difference is obvious, the statistical significance is achieved, and p<0.05，**p<0.01，***p<0.001, compared with rosemary extract group, honeysuckle extract group and ligusticum wallichii extract group, the composition group has obvious reduction of cell inflammatory factors and obvious statistical significance,#p<0.05，##p<0.01，###p<0.001. the combination of the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract can obviously inhibit inflammatory reaction of cells after the cells are irradiated by ultraviolet rays, and the anti-inflammatory effect after the combination is obviously superior to that of a single extract, so that the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract have obvious synergistic effect.
Example 11 comparison of the inhibitory effect of the composition on melanin content in B16F10 cells with rosemary extract, honeysuckle extract and chuanxiong rhizome extract.
Melanoma cells B16F10 were administered at 1X 10 per well4Cell densities of individuals were seeded in 96-well plates and experimental groups were as follows: and (3) a control group, a composition group (containing 0.25mg/mL rosemary extract, 0.25mg/mL honeysuckle extract and 0.25mg/mL ligusticum wallichii extract), a rosemary extract group (0.75mg/mL), a honeysuckle extract group (0.75mg/mL) and a ligusticum wallichii extract group (0.75mg/mL), discarding the original culture medium after 24 hours, replacing the phenol-free red culture medium containing the medicaments in each group, taking the supernatant of all the pore plates after 72 hours, and detecting the light absorption value of the pore plates at 490nm by using an enzyme-labeling instrument. The experiment was repeated 3 times. The results are shown in FIG. 11, andcompared with the control group, the cell-secreted melanin contents of the composition group, the rosemary extract group, the honeysuckle extract group and the ligusticum wallichii extract group are all reduced in a certain amount, but the melanin reduction of the composition group is most obvious, is obviously different from the control group, and has obvious statistical significance<0.01，***p<0.001. Compared with rosemary extract group, honeysuckle extract group and ligusticum wallichii extract group, the composition group also has obvious reduction of the cell melanin generation rate, the difference has statistical significance,#p<0.05，##p<0.01. the combination of the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract can obviously inhibit the generation and the secretion of melanin of melanoma cells, and the effect of inhibiting the generation of the melanin of the cells of the composition group is obviously better than that of a single extract, so that the composition has an obvious synergistic effect.
Example 12 formulation of blank cream and pharmaceutical cream.
The plant monomer composition with sunscreen effect of the embodiment is prepared by the following components in percentage by mass. The drug addition concentration was 40 times the cell use concentration.
In the embodiment, the composition of rosemary extract, honeysuckle extract and ligusticum wallichii extract is prepared into cosmetic ointment, wherein the rosemary extract comprises 1 percent, the honeysuckle extract comprises 1 percent, the ligusticum wallichii extract comprises 1 percent, and the basic formula comprises the following components in percentage by mass: 0.05% of disodium EDTA, 0.2% of allantoin, 6% of glycerol, 5% of mineral oil, 0.8% of glyceryl stearate, 4% of isopropyl palmitate, 3.5% of caprylic/capric triglyceride, 2% of steareth-2, 2% of steareth-21, 0.2% of methylparaben, 0.1% of propylparaben, 1.5% of cetostearyl alcohol, 1% of polyacrylate-13/polyisobutylene/polysorbate-20, 0.15% of bis (hydroxymethyl) imidazolidinyl urea, and the balance of water (the mass percentages are percentages of the total mass of the cosmetic cream). Both blank base creams and pharmaceutical creams were sampled by the cosmetics company according to the cosmetic preparation process and placed in cosmetic bottles.
The cream prepared by the embodiment is uniform in coating, fine in hand feeling and easy to absorb, has the faint fragrance of natural herbaceous plants, is mild and fresh, and can be used for human body tests.
Example 13 comparison of test results of the plant extract composition prepared according to the present invention on human arm skin before and after sun exposure.
Before the test, a skin elasticity moisture and melanin tester (Cutomer MPA 580, Germany) is used for detecting the basic values of moisture and melanin contents on the front surfaces of the left arm and the right arm of each person, and people without significant difference between the left arm and the right arm are selected to participate in the test, and 30 volunteers are used in total.
The test was conducted in summer, all volunteers were put on short-sleeved clothes, the test was started formally, two bottles of cosmetics were given to each volunteer, a blank cream of the basic formula of cosmetics was applied to the front of the left arm of the volunteer as a control (hereinafter referred to as control group), and a cosmetic cream containing the plant extract composition was applied to the front of the right arm as a test group (hereinafter referred to as test group). The tested part is kept dry and is prevented from contacting other external preparations, the external preparation is uniformly applied twice a day, about 0.5 g is applied for each time, and the skin reaction is observed after the external preparation is continuously used for 7 days without allergic symptoms such as erythema, edema, pimple and the like. The volunteers irradiate the arms of both hands coated with the cosmetic basic cream containing or not containing medicines under sunlight for at least half an hour every day, and after the test, the skin moisture content, skin elasticity and melanin content of the left and right arms are detected every week.
Each set of values (measurement data after experiment-measurement data before experiment)/measurement data before experiment × 100%.
The experimental result shows that no allergic symptoms such as erythema, edema, pimple and the like are observed on the arms of all the volunteers coated with the cosmetics of the test group. The experimental results also show that the percentage of melanin content significantly decreased with time before and after the arm test in the test group after smearing, and significantly differed from that before and after the control group test, as shown in table 1. The test group containing the sunscreen function has obvious effect, can obviously reduce melanin generation, resist skin tanning caused by ultraviolet irradiation, increase and keep skin moisture, promote skin elasticity to increase, has the function of resisting photoaging, has good protective effect on skin cells, and is a very ideal sunscreen and whitening cosmetic additive.
TABLE 1 Effect of plant extract composition on moisture content, elasticity and melanin content of human skin
Note: p <0.05, p <0.01, compared to control;
the protection of the present invention is not limited to the above embodiments. Variations and advantages that may occur to those skilled in the art may be incorporated into the invention without departing from the spirit and scope of the inventive concept, and the scope of the appended claims is intended to be protected.
Claims (10)
1. A plant extract composition for sun protection, after-sun repair, anti-inflammation, light aging prevention, whitening, moisture preservation or aging prevention is characterized in that the plant extract composition is composed of any two of rosemary extract, honeysuckle extract or ligusticum wallichii extract as active ingredients, and when the active ingredients are the rosemary extract and the honeysuckle extract, the mass part ratio of the two active ingredients is (3-9): (1-7); when the active ingredients are rosemary extract and ligusticum wallichii extract, the mass part ratio of the two active ingredients is (1-6): (4-9); when the active ingredients are honeysuckle extract and ligusticum wallichii extract, the mass part ratio of the two active ingredients is (1-6): (4-9);
or the plant extract composition is composed of rosemary extract, honeysuckle extract and ligusticum wallichii extract as active ingredients, and the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract are mixed according to the mass part ratio of (1-9): (1-9): (1-9).
2. The plant extract composition as claimed in claim 1, wherein the ratio of any two active ingredients of rosemary extract, honeysuckle extract or chuanxiong rhizome extract in parts by weight is (4-6): (4-6).
3. The plant extract composition as claimed in claim 1, wherein the ratio of the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract in parts by weight is 1: 1: 1.
4. a cosmetic composition comprising the plant extract composition according to any one of claims 1 to 3, wherein the active ingredient of any one of the extracts in the plant extract composition is 0.05% to 3% by weight of the cosmetic base, the balance being the cosmetic base.
5. The cosmetic composition of claim 4, wherein the active ingredient of any one of the extracts is 1% by weight of the cosmetic base.
6. Use of a plant extract composition according to any one of claims 1 to 3 or a cosmetic composition according to any one of claims 4 to 5 for the preparation of a product for combating damage to the skin by ultraviolet light and for repairing or preventing photoaging after damage.
7. Use of a plant extract composition according to any one of claims 1 to 3 or a cosmetic composition according to any one of claims 4 to 5 for the preparation of a cosmetic product having sunscreen, after-sun repair, anti-inflammatory, anti-photoaging, whitening, moisturizing or anti-aging effects.
8. Use of a plant extract composition according to any one of claims 1 to 3 or a cosmetic composition according to any one of claims 4 to 5 for the preparation of a product intended to prevent the deposition of melanin in the skin, or to reduce the production of melanin in skin cells, or to degrade already deposited melanin.
9. Use of a plant extract composition according to any one of claims 1 to 3 or a cosmetic composition according to any one of claims 4 to 5 for the preparation of an anti-inflammatory product for preventing, reducing or eliminating the inflammatory reaction of the skin.
10. Use of a plant extract composition according to any one of claims 1 to 3 or of a cosmetic composition according to any one of claims 4 to 5 for the preparation of a sunscreen, a photoaging or a depigmenting cosmetic.
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