KR100641302B1 - Cosmetic composition for reducing skin wrinkles comprisings asiaticoside - Google Patents

Abstract

A cosmetic composition comprising asiaticoside and asiatic acid is provided to have high stability and show excellent skin wrinkle improving effect without skin irritation. The cosmetic composition for improving skin wrinkles comprises asiaticoside and asiatic acid. The ratio of the asiaticoside to the asiatic acid is 1.5-2.5:1. Regarding the total weight of the composition, 0.5-1.0 wt.% of the asiaticoside and asiatic acid is contained.

Description

Cosmetic composition for improving skin wrinkles containing asiaticosides {Cosmetic composition for reducing skin wrinkles comprisings asiaticoside}

Figure 1 shows the results of the collagen biosynthesis promoting effect by the combination of various ratios of asiaticoside, asiatic acid, asiaticoside and asiatic acid (As: asiaticoside, Aa: asiatic acid).

Figure 2 shows the results of the collagenase activity inhibitory effect by the combination of various ratios of asiaticoside, asiatic, acid asiaticoside and asiatic acid (As: asiaticoside, Aa: asiatic acid).

Figure 3 shows the results of measuring the cell proliferation effect of human normal fibroblasts by the combination of various ratios of asiaticoside, asiatic acid, asiaticoside and asiatic acid (As: asiaticoside, FGF-2: fetal growth factor) -2 (fetal growth factor-2), Aa: asiatic acid).

The present invention relates to a cosmetic composition for improving skin wrinkles containing asiaticoside and asiatic acid in a ratio of 1.5 to 2.5: 1.

Human skin is changed by a number of internal and external factors as it ages. That is, internally, the secretion of various hormones that regulate metabolism decreases, immune cells function and activity decreases, and immunity is weakened. Externally, the frequency of exposure to ultraviolet rays increases due to the destruction of the ozone layer and environmental pollution. As the free radicals and active harmful oxygen increase, the skin wrinkles increase, the elasticity of the skin decreases, and blemishes, freckles, and black mushrooms increase. Methods for overcoming such skin aging are being studied.

In general, skin wrinkles are known to be produced by a number of factors, including skin moisture content, collagen content, and the ability to act on the environment. The greatest influence on the formation of double wrinkles is the amount of collagen produced and the amount and activity of collagenase, a collagen degrading enzyme.

Collagen, which is present in the dermal dermal layer, is known as a major ingredient that gives elasticity to the skin together with elastin, which accounts for about 70 to 80% of the total dry weight of the skin. Not only does collagen content decrease due to internal factors such as decreased cellular activity due to natural aging, but also collagen biosynthesis decreases due to external factors such as increased stress caused by various harmful environments and increased free radical species caused by sunlight. There is a problem that collagen decomposition is increased.

Efforts have been made to overcome such problems and to improve skin wrinkles. For example:

U.S. Pat.No. 5,340,568 describes a composition containing 2-deoxy-2-halo-lysophosphatidic acid that acts like a growth factor and induces cell proliferation. It is described as suppressing.

In addition, international patent WO97 / 39734 describes a method for alleviating skin aging using a composition comprising extracts of centellae herb, and WO01 / 78674 describes leucyanadines, centellae in extracts of Vitis vinifera. A skin aging improving composition comprising a combination of extracts of triterpenes and fish cartilage in an extract of Centella asiatica is described.

However, the skin wrinkle improvement active ingredients described above have various problems such as not being used as some cosmetic raw materials or being very unstable or not easily delivered to the skin.

Therefore, many studies have been conducted on active ingredients that can solve various problems and improve skin wrinkles.

The present inventors have diligently studied various substances to find cosmetics having high safety and stability and high antiwrinkle effect, and as a result, a composition containing asiaticoside and asiatic acid in a specific ratio, that is, 1.5 to 2.5: 1 ratio, In the case of administering these ingredients alone, the present invention was completed by confirming that the skin wrinkles were improved as well as safety and stability superior to those of different proportions.

Accordingly, it is an object of the present invention to provide a skin wrinkle improving cosmetic composition comprising asiaticoside and asiatic acid in a ratio of 1.5 to 2.5: 1 and cosmetics formulated with such compositions.

In one embodiment, the present invention relates to a skin wrinkle improving cosmetic composition comprising asiaticoside and asiatic acid in a ratio of 1.5 to 2.5: 1.

Both asiaticoside and asiatic acid contained in the composition of the present invention each have a structure in which five hexagonal rings are bonded. Asiaticoside has a complex structure in which sugar is attached to asiatic acid.

Asiaticoside and asiatic acid are natural substances contained in Centella asiatica, and have been widely used in skin diseases since they protect or improve the healing rate of burns and wounds (Shim et al, 1996. 6, 2937-2940, Bioorg.Med. Chem. Lett.). The pharmacological mechanisms of asiaticoside and asiatic acid compounds studied in 1990 are known to involve altering the production of extracellular matrix, including collagen, in skin cells.

The compositions of the present invention provide superior synergistically effective collagen biosynthesis promoting activity, inhibiting collagenase activity, and their respective combinations of asiaticosides and asiatic acids as well as their combinations of 1: 3, 1: 1, 1: 2, 3: 1 and Fibroblast proliferation activity was shown. Specifically, as shown in Figure 1, compared to the negative control, as for the 2: 1 blend of asiaticoside and asiatic acid 182%, Centella asiatica extract, asiaticoside alone, asiatic acid alone 119%, 124% , 127%, and the above-mentioned combinations exhibit collagen biosynthesis promoting activity of [1: 3 (152%), 1: 1 (148), 1: 2 (150%), 3: 1 (153%)], respectively. It was. As shown in Figure 2, compared to the control treated with TNF alpha alone, 48.6% of the 2: 1 combination of asiaticoside and asiatic acid, Centella extract, asiaticoside alone, asiatic acid alone was 18.1% and 19.6%, respectively. , 16.5%, and the combinations of the above ratios inhibit the collagenase activity of [1: 3 (33.9%), 1: 1 (32.2), 1: 2 (30.9%), 3: 1 (30.8%)), respectively. Indicated. As shown in FIG. 3, compared to the negative control group, the 2: 1 combination of asiaticoside and asiatic acid was 70.5%, FGF-2, Centella asiatica extract, asiaticoside alone, and asiatic acid alone, 32% and 22.2%, respectively. 21.2%, 25.2%, the above-mentioned combinations were fibroblast cells of [1: 3 (46.5%), 1: 1 (44.2%), 1: 2 (45.5%), 3: 1 (46.3%)], respectively. Proliferation efficiency was shown.

The synergistic skin wrinkle improvement effect of the composition of the present invention as described above is an effect shown for the same dosage (limiting the content of the wrinkle improvement component to 0.6% based on the total composition weight), When used in combination, the same or similar effects as the single use may be achieved. Nevertheless, the composition of the present invention showed an excellent skin wrinkle improvement effect as described above. In addition, the composition of the present invention showed excellent synergistic elasticity improvement not only in the in vitro test as described above, but also in the in vivo test measured skin elasticity. Excellent synergistic effects in both these in vitro and in vivo tests were possible because of the combination of asiaticoside and asiatic acid in certain proportions as in the present invention.

In a preferred embodiment the composition of the present invention comprises asiaticoside and asiatic acid in a ratio of 2: 1.

The ratio of asiaticoside and asiatic acid in the composition of the present invention is included in the ratio of 0.5 to 1.0% (w / w) based on the total weight of the composition. Preferably, asiaticoside and asiatic acid are included in a proportion of 0.5 to 0.7% (w / w), most preferably 0.6% (w / w).

The composition of the present invention may include adenosine, vitamin C, ursolic acid, and the like, in addition to including a combination of asiaticoside and asiatic acid in the above-mentioned ratios, and which do not have a detrimental effect on the skin wrinkle improvement effect. have.

The composition of the present invention can be applied to medicines, foods, cosmetics, etc. for improving skin wrinkles, and can take the form of oral (contents) or parenteral (external use). For oral use, for example, it may be formulated in the form of a pharmaceutical or food, parenteral (for external use), such as cosmetics, quasi-drugs, for example soap, skin adhesive patch, nutrition cream, It may be formulated as a topical formulation in the form of astringent lotion, flexible lotion, essence, nutrition gel, massage cream, powder, pack, and the like. These formulations can be combined with conventional carriers or additives well known in the art.

The composition of the present invention is preferably applied to cosmetics, including purified water, oils, surfactants, humectants, higher alcohols, chelating agents commonly used in the art for application to various forms of cosmetics, including the cosmetics described above , Pigments, fatty acids, antioxidants, preservatives, waxes, pH adjusters, fragrances and the like.

Among the above excipients, the oils include mineral oil, cyclomethicone, squalane, octyldodecyl myristate, olive oil, macadamia nut oil, glyceryl octanoate, castor oil, ethylhexyl isononanoate, dimethicone, and cyclic. Lofentasiloxane, sunflower seed oil, etc. are used, and as surfactant, sorbitan sesquinolate, polysorbate 60, glyceryl stearate, lipophilic glyceryl stearate, sorbitan oleate, sorbitan stearate, Die-cetylphosphate, sorbitan stearate / sucrosecoate, glyceryl stearate / polyethylene glycol-100 stearate, ceteareth-6 oleate, arachidyl alcohol / behenyl alcohol / arachidyl glucoside. Polypropylene glycol-26-butes-26 / polyethylene glycol-40 hydrogenated castor oil is used, and as a moisturizing agent, glycerin, sorbitol, propylene glycol, butylene glycol, hexylene glycol , Diglycerin, betaine, glycerin-26, methylgluses-20, etc. are used, and higher alcohols include cetyl alcohol, stearyl alcohol, octyldodecanol, isostearyl alcohol and the like. , Carbomer, magnesium aluminum silicate, cellulose gum, dextrin palmitate, and the like, and chelating agents include disodium ethane, tetrasodium ethane, and the like. Blue 1, Red 40, Yellow 4 , Yellow No. 5, etc. are used, and fatty acids are stearic acid, mystic acid, palmitic acid, isostearic acid, lauric acid, and the like. Cetate, butylated hydroxytoluene, and the like are used, and methyl paraben, butyl paraben, propyl paraben, phenoxyethanol, imidazolidinyl urea, chlorphenesin, and the like are used as preservatives, and carnauba wax, Candenilla wax, beeswax, braze, etc. are used, triethanolamine, citric acid, etc. may be used as a pH adjusting agent, and natural fragrance, combination fragrance, etc. may be used as a fragrance.

Therefore, in a preferred embodiment the present invention relates to a wrinkle improving cosmetic comprising the composition described above. In a more preferred embodiment, the above wrinkle improving cosmetics includes a nourishing cream, astringent lotion, a flexible lotion, an essence, a nourishing gel, a massage cream.

In one specific embodiment, the nourishing cream of the present invention comprises asiaticoside, asiatic acid, carbomer, xanthan gum, ceteareth-6 oleate, polysorbate 60, stearic acid, cetyl alcohol, cyclopentasiloxane, minerals Oil, sunflower seed oil, triethanolamine, tocophenylacetate, phenoxyethanol, combination perfume, purified water, and the like.

In another specific embodiment, the astringent lotion of the present invention may comprise asiaticoside, asiatic acid, propylene glycol, polypropylene glycol-26-butes-26 / polyethylene glycol-40 hydrogenated castor oil, ethanol , Phenoxyethanol, combination perfume, purified water and the like.

In another specific embodiment, the lotion of the present invention may be selected from the group consisting of asiaticoside, asiatic acid, ceteareth-6 oleate, arachidyl alcohol / behenyl alcohol / arachidyl glucoside, polydecene, disodium ethane, stearic acid, Carbomer, xanthan gum, phenoxyethanol, combination perfume, purified water, and the like.

In another specific embodiment, the essence of the present invention is asiaticoside, asiatic acid, glycerin, carbomer, xanthan gum, sunflower seed oil, beeswax, ceteareth-6 oleate, triethanolamine, preservatives, combination fragrance, purified water, etc. It may include.

In another specific embodiment, the nutrient gel of the present invention is asiaticoside, asiatic acid, glyceryl polymethacrylate / propylene glycol / methyl vinyl ether. Maleic Anhydride Copolymer, Glyceryl Polyacrylate / Dimethicone / Cyclomethicone, Polypropylene Glycol-26-Butes-26 / Polyethylene Glycol-40 Hydrogenated Castor Oil, Phenoxyethanol , Combination fragrances, purified water, and the like.

In another specific embodiment, the massage cream of the present invention is asiaticoside, asiatic acid, polysorbate 60, mineral oil, cyclopentasiloxane, carbomer, propylene glycol, triethanolamine, phenoxyethanol, combination perfume, purified water And the like.

The following examples illustrate the content of the invention, but the content of the invention is not limited thereto.

Example  One: Asiaticoside , Asiatico Sidewa Asian Formulation , And Asian  Collagen Synthesis Enhancement Effect

Human normal fibroblasts were inoculated into 6-well microplates containing DMEM medium (2 × 10 ⁵ cells / well) and incubated at 37 ° C. for 24 hours in a CO ₂ incubator at 5% concentration. After 24 hours, the medium was removed from each well and the content of asiaticoside (AG Scientific) and asiatic acid (AG Scientific) in DMEM containing 0.05% of FBS was 1.5 ug / ml: 4.5 ug / ml (1: 3), 3.0 ug / ml: 3.0 ug / ml (1: 1), 2 ug / ml: 4 ug / ml (1: 2), 4 ug / ml: 2 ug / ml (2: 1), 4.5 ug / ml: 1.5 ug / ml (3: 1) blend of asiaticoside and asiatic acid, asiaticoside (6 ug / ml), asiatic acid (6 ug / ml), centella asiatica extract (6 ug / ml) ( Handan Fine Chemicals) and the like, and then incubated again for 24 hours. After 24 hours, cell media were collected to prepare samples.

The amount of collagen synthesized was determined using the collagen measurement kit (Procollagen Type I C-peptide EIA kit (MK101), Takara, Kyoto, Japan). The amount of collagen type I C-peptide (Type I C-peptide; PICP) Was measured. After diluting the standard solution included in the collagen kit, absorbance was measured at 450 nm to prepare a standard concentration curve. 100 ul of an antibody solution with POD enzyme was added to each well, and 20 ul of the supernatant of the sample and the standard solution diluted by concentration were added and mixed. The mixture was left at 37 ° C. for 3 hours, after which the contents were all removed and each well was washed four times with 400ul of PBS. 100 ul of substrate solution was added to the wells, and then left at room temperature for 15 minutes. In order to stop the reaction, 100ul of 1N sulfuric acid solution was added and mixed, and the absorbance was measured at 450nm. This experiment was conducted twice at the same time to find the average value. PICP amount was calculated by substituting the absorbance of the sample in the standard concentration curve and converted to ng / 2 × 10 ⁵ cells.

As a result, when the mixture of asiaticoside and asiatic acid was treated, the collagen production rate was higher than when the centella extract, asiaticoside and asiatic acid were treated alone. In particular, when the ratio of asiaticoside and asiatic acid is 2: 1, it showed the highest collagen production rate in human normal fibroblasts. The results are shown in FIG.

Example  2: Asiaticoside , Asiatico Sidewa Asian Formulation , And Asian  Inhibitory effect on collagenase activity

Human normal fibroblasts were inoculated into 6-well microplates containing DMEM medium (2 × 10 ⁵ cells / well) and incubated at 37 ° C. for 24 hours in a CO ₂ incubator at 5% concentration. After 24 hours, the media was removed from each well and then serum-starvation was induced for 16 hours in DMEM medium containing FBS 0.05%. After 16 hours, TNF-alpha (20ng / ml) alone or as content of asiaticoside and asiatic acid was 1.5 ug / ml: 4.5 ug / ml (1: 3) and 3.0 ug / ml: 3.0 ug / ml (1 : 1), 2 ug / ml: 4 ug / ml (1: 2), 4 ug / ml: 2 ug / ml (2: 1), 4.5 ug / ml: 1.5 ug / ml (3: 1) A combination of tycoside and asiatic acid, asiaticoside (6 ug / ml), asiatic acid (6 ug / ml), Centella asiatica extract (6 ug / ml), and the like were treated together for 24 hours. After 24 hours, cell media were collected to prepare samples.

An antibody against collagenase was used to measure the activity of collagenase, an enzyme that degrades collagen. In this test, a type I collagenase assay kit (Amersham Biosciences, RPN2629) was used, and the absorbance was measured by an ELISA reader. The measured standard values were expressed in the form of mean + standard deviation, and the significance was tested by the t-test using the SPSS / PC + program. When the ratio of asiaticoside to asiatic acid is 2: 1, the normal value of human The highest percentage of collagenase activity was inhibited in fibroblasts. These results indicated that the experimental group treated with the combination of asiaticoside and asiatic acid showed significantly increased MMP-1 activity in TNF alpha than when treated with the same concentration of Centella asiatica extract, asiaticoside and asiatic acid, respectively. Decrease. The results are shown in FIG.

Example  3: Asiaticoside , Asiatico Sidewa Asian Formulation , Asiatican  Cell proliferation effect of human normal fibroblasts

The content of asiaticoside and asiatic acid in human normal fibroblast culture was 1.5 ug / ml: 4.5 ug / ml (1: 3), 3.0 ug / ml: 3.0 ug / ml (1: 1) and 2 ug, respectively. / ml: 4 ug / ml (1: 2), 4 ug / ml: 2 ug / ml (2: 1), 4.5 ug / ml: 1.5 ug / ml (3: 1) After testing the cells to test for cell proliferative effects on the formulation, asiaticoside (6 ug / ml), asiatic acid (6 ug / ml), centella asiatica extract (6 ug / ml), the cells were serum-free. The proliferation effect of the cells by culturing was measured using the MTT (3- (4,5-dimetylthiazol-2-yl) 2,5-diphenyltetrazolium bromide) assay method. MTT is a pale yellow substrate that is cleaved by respiratory chain enzymes in mitochondria of living cells and produces dark blue formazan. Since no reaction occurs in dead cells, the amount of formazan produced is used to determine the number of living cells. As a result, when the combination of asiaticoside and asiatic acid was treated, the cell proliferation effect was better than when the same concentration of Centella asiatica extract, asiaticoside, or asiatic acid was treated. In particular, when the ratio of asiaticoside and asiatic acid is 2: 1, it showed the highest cell proliferation effect in human normal fibroblasts (FIG. 3). These results indicate that the cell proliferation effect of asiatic acid and asiaticoside has the highest synergy at a certain ratio.

Example  4: Production Example

A. Nutrition Cream

An example of preparing a nutritional cream containing asiaticoside and asiatic acid was prepared by a conventional method with the composition of Table 1 below.

Ingredients and content of nutrition cream (% by weight) ingredient Preparation Example 1 Preparation Example 2 Preparation Example 3 Preparation Example 4 Preparation Example 5 Preparation Example 6 Preparation Example 7 Preparation Example 8 Asiaticoside Powder 0.6 0.0 0.30 0.4 0.2 0.45 0.15 0.0 Asiatic acid 0.0 0.6 0.30 0.2 0.4 0.15 0.45 0.0 Centella asiatica extract 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.6 Carbomer 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 Xanthan Gum 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 Ceteares-6 Olivet 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Sorbitan stearate / sucrose cocoate 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Polysorbate 60 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Glyceryl Stearate / Pig-100stearate 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Stearic acid 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Cetyl alcohol 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Die-cetylphosphate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Beeswax 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Cyclopentasiloxane 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 Mineral oil 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Sunflower Seed Oil 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Dimethicone 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Triethanolamine 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 Tocopheryl Acetate 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 antiseptic a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount Spices a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount Purified water Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Sum 100 100 100 100 100 100 100 100

B. Converging Cosmetics

A preparation example of astringent lotion containing asiaticoside and asiatic acid was prepared by a conventional method with the composition of Table 2 below.

Components and Contents of Converging Cosmetics (wt%) ingredient content Asiaticoside Powder 0.40 Asiatic acid 0.20 Propylene glycol 4.00 Phenyltrimethicone 1.00 Fiji-26-Bootes-26 / PG-40 Hydrogenated Castor Oil 0.50 Fiji-8 Dimethicone 1.50 ethanol 6.00 Disodium ID 0.10 Butylene Glycol 3.00 Triethanolamine 0.15 antiseptic a very small amount Spices a very small amount Purified water Remaining amount Sum 100

C. Lotion

A preparation example of a lotion containing asiaticoside and asiatic acid was prepared by a conventional method with the composition of Table 3 below.

Ingredients and content (% by weight) of lotions ingredient content Asiaticoside Powder 0.40 Asiatic acid 0.20 Ceteares-6Olivate 3.00 Arakidil Alcohol / Behenyl Alcohol / Arachidyl Glucoside 1.00 Tocopheryl Acetate 0.10 Neopentylglycoldiheptanoate 3.00 Polydecene 2.00 Stearic acid 0.40 Dimethicone 0.50 Triethanolamine 0.20 Disodium ID 0.30 Carbomer 0.40 Xanthan Gum 0.06 antiseptic a very small amount Spices a very small amount Purified water Remaining amount Sum 100

D. Essence

An example of preparing an essence containing asiaticoside and asiatic acid was prepared by a conventional method with the composition of Table 4 below.

Essence Components and Content (wt%) ingredient content Asiaticoside Powder 0.40 Asiatic acid 0.20 glycerin 5.0 Butylene Glycol 9.0 Carbomer 0.20 Xanthan Gum 0.06 Sunflower Seed Oil 4.0 Cyclomethicone 2.0 Beeswax 0.60 Arachidil Alcohol / Behenyl Alcohol / Arachidyl Glucoside 1.50 Ceteares-6 Olivet 4.0 Triethanolamine 0.18 antiseptic a very small amount Spices a very small amount Purified water Remaining amount Sum 100

E. Nutrition Gel

An example of preparing a nutritional gel containing asiaticoside and asiatic acid was prepared by a conventional method with the composition of Table 5 below.

Ingredients and content of nutrient gel ingredient content Asiaticoside Powder 0.40 Asiatic acid 0.20 Glyceryl Polymethacrylate / Propylene Glycol / FIG M.E.Polymer 2.00 Acrylate / C10-30 Alkylacrylate Crosspolymer 0.70 Glyceryl Polyacrylate / Dimethicone / Cyclomethicone 4.00 Fiji-26-Bootes-26 / Pig-40 Hydrogenated Castor Oil 10 Triethanolamine 1.00 antiseptic a very small amount Spices a very small amount Purified water Remaining amount Sum 100

F. Massage Cream

A preparation example of a massage cream containing asiaticoside and asiatic acid was prepared by a conventional method with the composition of Table 6 below.

Ingredients and content (% by weight) of massage cream ingredient content Asiaticoside Powder 0.40 Asiatic acid 0.20 Butylene Glycol 2.00 Polysorbate 60 1.50 Sorbitan sesquioleate 0.80 Mineral oil 40.0 Cyclopentasiloxane 8.00 Caprylic / Capric Triglycerides 4.00 Carbomer 0.20 Fiji-20 sorbitan oleate 1.00 Propylene glycol 3.00 Triethanolamine 0.43 antiseptic a very small amount Spices a very small amount Sum 100

G. Comparison Production Example : Purified water  Nutritional Cream Containing

Formulation example of the cream substitute in purified water without the addition of asiaticoside and asiatic acid in Preparation Example A is as follows.

Ingredients and content of cream (% by weight) ingredient content Asiaticoside Powder 0.00 Asiatic acid 0.00 Carbomer 0.20 Xanthan Gum 0.08 Ceteares-6 Olivet 2.0 Sorbitan stearate / sucrose cocoate 2.0 Polysorbate 60 1.0 Glyceryl Stearate / Pig-100stearate 1.0 Stearic acid 1.0 Cetyl alcohol 1.5 Die-cetylphosphate 0.5 Beeswax 1.5 Cyclopentasiloxane 7.0 Mineral oil 2.0 Sunflower Seed Oil 2.0 Dimethicone 3.0 Triethanolamine 0.20 Tocopheryl Acetate 0.10 antiseptic a very small amount Spices a very small amount Purified water Remaining amount Sum 100

Example  5: Asiaticoside , Asiatic acid , Asiatico Sidewa Asian Combination of  Safety confirmation experiment on human skin

In order to confirm that the combination of asiaticoside, asiatic acid, asiaticoside and asiatic acid is safe for human skin, skin safety verification experiments were performed. As a test method, a skin cumulative stimulation test was performed.

Based on squalene, a formulation containing asiaticoside, asiatic acid, or a combination of asiaticoside and asiatic acid was prepared, and was used to accumulate nine 24 hours every other day on the upper forearm for 30 healthy adults. By applying a patch, it was determined whether Centella asiatica extract (90% asiaticoside content) irritated the skin.

The patching method used a fin chamber (Finn chamber, Epitest Ltd, Finland). 15ul of each said skin external preparation was dripped at the chamber, and the patch was performed. The degree of response to the skin each time was scored using the following formula, and the results are shown in Table 7 below.

Average responsiveness = [[response index x responsiveness / total number of subjects x highest score (4 points)] x 100] ÷ number of tests (9 times)

At this time, ± 1 point, + 2 points, + + 4 points in the reactivity, it is determined as a safe composition when the average reactivity is less than 3.

In the case of Table 8, in the case of the control group, the number of people corresponding to ±, +, ++ at week 1 was 2, 0, 0, respectively, and the rest did not appear. When calculated according to the formula described above, the average reactivity of all the test groups was 0.18, so the value was 3 or less, which was determined to be a safe composition.

As shown in Table 8, the combination of asiaticoside, asiatic acid, asiaticoside and asiatic acid did not show a distinct cumulative stimulation pattern and was determined to be safe for human skin.

Example  6: Asiaticoside , Asiatic acid , Asiatico Sidewa Asian Blend  Containing Cosmetic  Wrinkle improvement effect measurement

Nourishing creams prepared in Preparation Examples 1 to 8 and Comparative Preparation Example (nutrient cream containing purified water) were used. Wrinkle improvement effect was evaluated by measuring skin elasticity change. The skin elasticity was measured in 30 healthy women (25 to 35 years old) who were constantly maintained at a temperature of 24 to 26 ° C. and a humidity of 38 to 40%. After using twice a day for 3 months, measured by using a cutometer SEM 474, the criterion was compared with the relative value of 0 for no skin elasticity, 5 for many cases. The test results are shown in Table 9 below.

Improvement of skin elasticity of nutritional cream containing a combination of asiaticoside, asiatic acid, asiaticoside and asiatic acid Test Items Preparation Example 1 Preparation Example 2 Preparation Example 3 Comparative Preparation Example (Asiaticoside, Asiantic Acid Free Cream) Skin elasticity 4.1 4.2 5.5 1.45  Test Items Preparation Example 4 Preparation Example 5 Preparation Example 6 Preparation Example 7 Preparation Example 8 Skin elasticity 6.5 5.5 5.7 5.6 4.0

As shown in Table 9, in the case of Preparation Example 4 containing asiaticoside and asiatic acid in a ratio of 2: 1, as well as comparative preparations containing no asiaticoside and asiatic acid, different ratios of asiaticoside It can be seen that there is a synergistic effect with the skin elasticity significantly superior to the blend of and asiatic acid.

Wrinkle improvement cosmetic composition containing asiaticoside and asiatic acid in a ratio of 1.5 to 2.5: 1 of the present invention, showed excellent synergistic wrinkle improvement effect in both in vitro and in vivo tests, irritation to the skin There is little, and it can use as a raw material for new wrinkle improvement.

Claims (6)

A skin wrinkle improvement cosmetic composition comprising asiaticoside and asiatic acid in a ratio of 1.5 to 2.5: 1. The composition of claim 1 comprising asiaticoside and asiatic acid in a ratio of 2: 1. The composition of claim 1 or 2, wherein the asiaticoside and asiatic acid are from 0.5 to 1.0% (w / w) based on the total weight of the composition. The composition of claim 2 wherein the asiaticoside and asiatic acid are from 0.5 to 0.7% based on the total weight of the composition. Skin wrinkle improvement cosmetics comprising the composition of claim 1. The cosmetic according to claim 5, which is a nourishing cream, astringent lotion, a flexible lotion, an essence, a nourishing gel or a massage cream.

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