CN110787119A

CN110787119A - Antibacterial skin-care traditional Chinese medicine composition and preparation method and application thereof

Info

Publication number: CN110787119A
Application number: CN201911189605.9A
Authority: CN
Inventors: 王珍明; 徐尧; 刘伟毅
Original assignee: SHANGHAI SOAPMAKING CO Ltd
Current assignee: SHANGHAI SOAPMAKING CO Ltd
Priority date: 2019-11-28
Filing date: 2019-11-28
Publication date: 2020-02-14

Abstract

The invention relates to an antibacterial skin-care traditional Chinese medicine composition, a preparation method and application thereof, wherein the composition is a purified product extracted and purified from seven white formulas which are composed of the following seven raw materials: 60-100 parts of angelica dahurica, 60-100 parts of bighead atractylodes rhizome, 60-100 parts of ampelopsis japonica, 60-100 parts of white poria, 60-100 parts of cortex dictamni, 60-100 parts of rhizoma typhonii and 6-10 parts of pearl. Compared with the prior art, the invention has the effects of resisting bacteria, whitening skin, removing freckles and the like.

Description

Antibacterial skin-care traditional Chinese medicine composition and preparation method and application thereof

Technical Field

The invention relates to the field of cosmetics, in particular to an antibacterial skin-care traditional Chinese medicine composition for cosmetics such as medicated soap and washing.

Background

Along with the improvement of living standard of people, the requirements of people on self conditions are increasingly improved, and the emphasis on skin care is also increasingly high. The demand for cleansing products is no longer limited to cleansing, but rather, it is desirable to have more skin care efficacy properties while cleansing the skin. At present, a lot of facial cleanser and solid soap type facial cleaning products appear on the market, and some products have good cleaning capability, but have strong alkalinity and large irritation to skin, so that the skin can be tense after washing; some products have good cleaning effect, are mild and non-irritant, but have single function; some products are added with chemical components which are not beneficial to human bodies, such as synthetic perfume, silicon, viscous agent, foaming agent, emulsifier and the like, and are easy to cause damage to the skin.

Therefore, the Chinese patent application CN201910450929.7 discloses a sterilizing and itching relieving medicinal soap which is characterized by comprising ozone salt, fatty acyl hydroxyethyl sodium sulfonate and the following raw materials in parts by weight: 5-10 parts of lithospermum oil, 2-6 parts of evening primrose oil, 3-8 parts of rose essential oil, 4-8 parts of rhodiola rosea essential oil, 5-8 parts of wormwood essential oil, 0.5-10 parts of giant salamander mucus extract, 1-5 parts of tremella polysaccharide, 20-35 parts of itching relieving traditional Chinese medicine water extract, 20-30 parts of cocamidopropyl betaine, 25-40 parts of foaming agent, 150-250 parts of cocoyl hydroxyethyl sodium sulfonate and 50-75 parts of soap particles; although the disinfectant has the effects of sterilizing and relieving itching, the disinfectant has various formula components and complex preparation method, contains partial chemicals and has great side effect.

Patent application CN201710893387.1 discloses a plant extract for whitening skin and resisting wrinkles and a preparation method thereof, the plant extract is prepared by using rhodiola rosea, helichrysum, camellia, rhizoma typhonii, bletilla striata, ginseng, safflower, radix paeoniae alba, radix angelicae, bighead atractylodes rhizome, rose, grape seeds and sea buckthorn as raw materials and adopting a preparation process of extracting with ethanol; the invention can effectively inhibit the generation of melanin, accelerate the metabolism of the melanin, improve the problems of rough skin and color spots, promote microcirculation, accelerate the renewal of skin cells, contribute to the synthesis of collagen, improve dark yellow complexion, relieve epidermal skin and smooth wrinkles, and can be prepared into facial cleanser, toning lotion, essence, cream, eye cream, facial mask, hand cream and body lotion. Although the extract disclosed in the patent can achieve whitening and antibacterial effects, the raw materials are complex, part of the raw materials are expensive, the popularization is not facilitated, and in addition, the function is single, and the effect is poor.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provide an antibacterial skin-care traditional Chinese medicine composition, and a preparation method and application thereof.

The purpose of the invention can be realized by the following technical scheme: the antibacterial skin-care traditional Chinese medicine composition is characterized by being a purified product extracted and purified from a seven-white formula consisting of the following seven raw materials, wherein the seven-white formula comprises the following components in parts by weight: 60-100 parts of angelica dahurica, 60-100 parts of bighead atractylodes rhizome, 60-100 parts of ampelopsis japonica, 60-100 parts of white poria, 60-100 parts of cortex dictamni, 60-100 parts of rhizoma typhonii and 6-10 parts of pearl.

The composition is a seven-white formula consisting of the following seven raw materials: 80-85 parts of angelica dahurica, 80-85 parts of bighead atractylodes rhizome, 80-85 parts of ampelopsis japonica, 80-85 parts of white poria, 80-85 parts of cortex dictamni, 80-85 parts of rhizoma typhonii and 8-9 parts of pearl.

The pearls are white pearls which are crushed to be 500-600 meshes.

The preparation method of the antibacterial skin-care traditional Chinese medicine composition comprises the following steps:

(1) extraction of

Weighing 60-100 parts by weight of radix angelicae, 60-100 parts by weight of bighead atractylodes rhizome, 60-100 parts by weight of radix ampelopsis, 60-100 parts by weight of poria cocos, 60-100 parts by weight of cortex dictamni and 60-100 parts by weight of rhizoma typhonii, adding water for decoction, extracting for 2 times, adding water for the first time, wherein the water amount is 6-10 times of the total weight of the six components, decocting for 0.5-2 hours, adding water for the second time, wherein the water amount is 6-10 times of the total weight of the six components, decocting for 0.1-1 hour, filtering, combining filtrates, and concentrating to 0.5-2 g crude drug/mL at normal pressure for later use;

(2) purification of

Adding ethanol with the weight being 1-3 times that of the water extract concentrated solution obtained in the step (1), stirring while adding, standing for 10-15 hours, separating supernatant, concentrating under reduced pressure until no alcohol smell exists, concentrating under reduced pressure at 50-80 ℃ to 0.5-2 g crude drug/ml, adding 6-10 parts by weight of pearl, and fully mixing.

The water added in the step (1) is deionized water.

The composition product obtained in the step (2) contains 0.1-1 wt% of saponin and 0.1-1 wt% of glucoside.

The composition product obtained in the step (2) contains 0.1-1 wt% of SOD (superoxide dismutase) components, wherein the SOD components comprise three trace elements of manganese, copper and zinc.

The application of the antibacterial skin-care traditional Chinese medicine composition is characterized in that the purified products (namely the traditional Chinese medicine composition) obtained by extracting and purifying seven components in the seven-component formula are used in cosmetics or washing products, the cosmetics comprise facial cleanser, cosmetic water, essence, cream, eye cream, facial mask, hand cream, body lotion and the like, and the washing products comprise perfumed soap, medicated soap, laundry detergent and the like.

The seven-white prescription of the invention adopts seven white traditional Chinese medicines, so that the components have synergistic effect and obtain good antibacterial and skin-care effects:

1. root of Dahurian Angelica

The product is dried root of Angelica dahurica (Fisch. ex Hoffm.) Benth.ethook.f. or Angelica dahurica (Fisch. ex Hoffm.) Benth.ethook.f. var. fortmosana (Boiss.) Shann et Yuan. The product has effects in relieving exterior syndrome, dispelling cold, expelling pathogenic wind, relieving pain, dredging nasal orifice, eliminating dampness, relieving leukorrhagia, and promoting pus discharge, and can be used for treating common cold, headache, supercilium pain, nasal obstruction, nasal discharge, allergic rhinitis, nasosinusitis, toothache, leukorrhagia, and sore and ulcer with swelling and pain.

The radix Angelicae Dahuricae contains volatile oil, 29 compounds with high contents of methylcyclodecane, 1-tetradecene, ethyl laurate, and furocoumarin are detected in the volatile oil. In addition, it also contains imperatorin, angelicin, scopoletin, byak-angelicin, isoimperatorin, hydrated byak-angelicin, iso-hui-angelicin, oxypeucedanin, anethole, carvacrol, palmitic acid, etc.

The traditional Chinese medicine considers that the angelica dahurica is warm in nature and pungent in taste, enters the channels of the lung, the spleen and the stomach, dispels wind-damp, promotes blood circulation, expels pus, promotes granulation and relieves pain. Can be used for treating headache, toothache, nasosinusitis, hemorrhoid, leucorrhea with red and white discharge, carbuncle, cellulitis, pyocutaneous disease, skin pruritus, relieving exterior syndrome, dispelling cold, dispelling pathogenic wind, relieving pain, relieving nasal obstruction, eliminating dampness, stopping leukorrhagia, and relieving swelling and pus discharge.

Pharmacological research shows that the water decoction also has inhibitory effect on various bacteria such as Escherichia coli, Shigella sonnei, Proteus, typhoid bacillus, paratyphoid bacillus, Pseudomonas aeruginosa, cholera bacillus, etc. It also has effect in inhibiting human type tubercle bacillus, and the methanol extract has radioprotective effect. In addition, the composition has antipyretic, analgesic and anti-inflammatory effects; shortening blood coagulation time, dilating coronary blood vessel, and lowering blood pressure; photosensitization; has antimicrobial effect.

2 white atractylodes rhizome

The product is named as English: RHIZOMAATRACTYLODIS MACROCEPHHAAE is prepared from Atractylodes macrocephala of Compositae, and has gray yellow or gray brown surface, nodular protrusion, intermittent longitudinal wrinkle and furrow, fibrous root scar, and residual stem base and bud scar at top. Hard and hard, not easy to break, uneven section, yellowish white to light brown, and brownish yellow point-shaped oil chamber dispersed; the dried cutin has darker color or cracks. Fragrant smell, sweet and slightly pungent taste, and slightly sticky when chewed.

The white atractylodes rhizome mainly contains 1.4 percent of volatile oil, contains 1.4 percent of Atractylol (atractyl ol), atractylone (atractylenon) and the like as main components, and also contains vitamin A, the rhizome contains about 1.4 percent of volatile oil, the oil contains atractylone (a-tetractylenone), and also contains atractylenolide A, B, apine dienone [ selina-4(14), 7(11) -dienone ], β -terpinene (β -Se-ene), eucalyptol (eucalyptus-terpene), oxygen coumarines (oxicoumarines), saccharides, resins and the like.

The white atractylodes rhizome has special effects on whitening skin. It has inhibitory effect on dermatophytosis such as dermatophytosis. Ancient book "medical property treatise": "have beautiful main face, retain youthful looks and remove spots. White atractylodes rhizome is dipped in wine (or vinegar) in the form of ground ink and is uniformly applied to the face, so that the face-beautifying wine can whiten the skin, clear heat and dry dampness, kill mites and remove acnes, and can treat freckles and black spots. The Sheng Yi Li Shizhen says that the formula treats the freckles' extremely. The widely spread traditional Chinese medicine beautifying formula Saibai soup recorded in Ming Dynasty (16 th century) medical works "medical entry" is described as follows: the white atractylodes rhizome can tonify qi and blood, whiten and moisten skin, and is suitable for rough skin, sallow complexion, chloasma, pigmentation and the like caused by deficiency-cold of qi and blood. In traditional Chinese medicine, whether the skin of a person is beautiful or not is considered to have close relationship with the functions of the viscera, and if the viscera are diseased and qi and blood are not harmonized, the skin is rough and spots are generated on the face. Therefore, the formula starts with the functions of regulating qi and blood and regulating the five internal organs, thereby whitening and removing freckles.

3 Ampelopsis japonica

The product has the name of Ampelopsis japonica: japanese Ampelopsis Root. Root tuber of Ampelopsis japonica (Thunb.) Makino, a family Vitaceae. And (5) making the vine. The stem is branched with pale purple color, scattered and punctate skin pores, and the tendrils and leaves are opposite. Palm compound leaves grow alternately, 3-5 small leaves, part of the leaves are divided into pinnate parts, part of the leaves are carved, the edges are thinned with coarse saw teeth, the leaf axis is provided with wide wings, joints are arranged at the base parts of the divided leaves, and both sides are hairless. The parasol inflorescence and the leaves are opposite, and the inflorescence peduncle is slender and wound; small flower, sepal 5; petal 5, light yellow; a stamen 5; the flower disc is cup-shaped with slightly split edges. Berry is spherical or kidney-shaped, blue or white when cooked, and has pinhole-shaped pits.

The physical and chemical properties and spectral analysis of the Ampelopsis japonica (Thunb.) are identified as physcion, lupeol, β -sitosterol, chrysophanol, hexadecanoic acid, emodin, fumaric acid, daucosterol, gallic acid and dulcitol, wherein physcion, chrysophanol, emodin, fumaric acid and dulcitol are firstly obtained from the Ampelopsis.

The ampelopsis japonica contains a large amount of mucus and starch, has fine white matter, has excellent beautifying effect, can eliminate facial acne, can eliminate stagnation and relieve pain, can also eliminate dermatitis, can reduce the harm of fungi to human skin, and can also eliminate facial acne and color spots. The ampelopsis japonica can lighten spots and whiten the skin, twenty grams of the ampelopsis japonica can be added into nine grams of biond magnolia flower and thirty-ten grams of white gourd tripe, fifteen grams of angelica and fifteen grams of flour after the color spots appear in normal times, the traditional Chinese medicines are ground, uniformly mixed and decocted to obtain medicinal liquid, the medicinal liquid is added into the flour to be mixed into paste to be directly applied externally, three to four times per week, the color spots can be lightened quickly, and the whitening effect is very obvious.

4 white poria

The product is dried cortex of Wolf sclerotium of Poria cocos Cos (Schw.) belonging to Polyporaceae. Digging more than 7-9 months, collecting the cut skin when processing Poria slices and Poria blocks, and drying in the shade. The product is in the form of strip or irregular pieces with different sizes. The outer surface is tan to black, with warty processes, and the inner surface is light brown and often has a white or light red subcutaneous part. It is soft and slightly elastic. Light smell, light taste, sticky teeth when chewed.

The Poria cocos comprises Isopolysaccharide (pachyman), Isoacid (pachymacic), baicalein (tumulosicacrl), buccolinic acid (eburicoxicalcl), pinicolic acid (pinicolic), ergosterol (ergosterol), etc.

The tuckahoe has the effects of excreting dampness and promoting diuresis, tonifying spleen and stomach, calming heart and soothing nerves. The antibacterial effect of the white poria is as follows: the 100% decoction of Poria has inhibitory effect on Staphylococcus aureus, Escherichia coli, and Proteus by plate punching. The result of the test on the bacteriostasis by the test tube method is that the tuckahoe has no bacteriostasis. The ethanol extract of Poria was found to kill leptospira in vitro, but the water decoction was not effective.

5 cortex Dictamni Radicis

The product is dried root bark of Dictamnus dasycarpus Turcz of Rutaceae. Digging root in spring and autumn, removing silt and rough bark, stripping root bark, and drying. The product is in the shape of a reel, and has a length of 5-15 cm, a diameter of 1-2 cm, and a thickness of 0.2-0.5 cm. The outer surface is gray white or light gray yellow, has fine longitudinal wrinkles and fine root marks and is often provided with protruded granular small points; the inner surface is white-like and has fine longitudinal lines. The product is crisp, dust flies when broken, the section is uneven and is slightly lamellar, and the outer layer is peeled off, so that a small bright spot which flickers when meeting light can be seen. Has smell of mutton and slightly bitter taste.

The cortex Dictamni Radicis contains Dictamnine (Dictamnine), Trigonelline (Trigonelline), Choline (Choline), dictamnolide (Dic-tamnolactone, Objunactone), dictamnolide (Dictam-lactone), Sitosterol (Sitosterol), Fraxinellone (Fraxinellone), obanone (Objunone), and phellodendron ketoacid (Objunonica). Also contains fatty acid and crude saponin.

Cortex dictamni is a commonly used traditional Chinese medicine. Has effects in clearing away heat, eliminating dampness, dispelling pathogenic wind, and removing toxic substances. It is commonly used for treating damp-heat sore, yellow water dripping, eczema, scabies, sore, scabies, rheumatism and pyretic arthralgia. The cortex Dictamni Radicis has effects of dispelling pathogenic wind, eliminating dampness, clearing heat, and removing toxic substance. Cortex Dictamni Radicis has effects of clearing heat and detoxicating, eliminating dampness and relieving itching in dermatology, can be used for treating dermatogic diseases such as tinea pedis, verruca plana, skin pruritus, urticaria, eczema, etc., and has good antiallergic effect and itching relieving effect. Antibacterial and antipruritic effects, and cortex Dictamni Radicis has broad-spectrum fungus inhibiting effect. The action mechanism is that dictamnine influences the normal synthesis of cell genetic materials, so that the dictamnine cannot complete normal cell cycle and has no influence on sterol biosynthesis pathway, and traditional antifungal agents are all used for synthesizing ergosterol biosynthesis inhibitors. The cortex Dictamni Radicis extract has antipruritic effect. Pruritus is a common symptom of skin diseases. Histamine, one of the inflammatory mediators released by mast cell activation degranulation due to a recognized allergy, can cause skin itching, and anti-itching is also part of the treatment of skin diseases.

6 rhizoma Typhonii

Giant typhonium rhizome, the name of traditional Chinese medicine. Is a dried tuber of Typhonium giganteum Engl. of Araceae. Collected in autumn, removed fibrous root and peel, and dried in the sun. Has effects of dispelling pathogenic wind and phlegm, arresting convulsion, removing toxic substance, resolving hard mass, and relieving pain. Can be used for treating phlegm accumulation due to apoplexy, facial paralysis, slurred speech, convulsion, epilepsy, tetanus, phlegm syncope, headache, lymphoid tuberculosis, subcutaneous nodule, and venomous snake bite.

Rhizoma Typhonii contains β -sitosterol and its glucoside, meso-inositol, mucus, sucrose, and optionally saponin, and optionally choline, uracil, succinic acid, palmitic acid, linoleic acid, oleic acid, rhizoma Typhonii lectin, rhizoma Typhonii choline, trilinoleic acid, and dipalmitoyl glyceride.

The rhizoma Typhonii contains saponin, glucoside, etc., and has effects of eliminating melanin pigmentation on face, whitening skin, and removing freckle, acne, and erythra. Dispelling pathogenic wind and phlegm, arresting convulsion, removing toxic substance, resolving hard mass, and relieving pain.

7 Pearl

The Margarita product is prepared from bivalves such as Hyriopisi cumini (Lea) or Cristaria plicata (Leach) of Pinctada of Margaritifera. Taking out from animal body, cleaning, and drying. Has the effects of tranquilizing, arresting convulsion, improving eyesight, removing nebula, removing toxic substance, promoting granulation, moistening skin, and removing speckle. Can be used for treating pavor, insomnia, infantile convulsion, epilepsy, conjunctival congestion, nebula, unhealing sore and ulcer, and skin mottle.

The organic substance in Margarita contains 17 amino acids such as aspartic acid, threonine, serine, glutamic acid, glycine, alanine, cystine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, histidine, arginine, and proline. In addition, it also contains more than 30 kinds of trace elements, taurine, abundant vitamins and peptides.

The pearl contains three trace elements of manganese, copper, zinc and the like which are components for forming SOD, and the pearl powder is coated on the surface of the pearl, which can promote the activity of superoxide dismutase (SOD) of human skin, inhibit the synthesis of melanin and keep the skin white. In addition, because SOD has the function of scavenging free radicals, the pearl powder can prevent skin aging and wrinkling when being coated on the surface. Has the effects of tranquilizing, arresting convulsion, improving eyesight, removing nebula, removing toxic substance, promoting granulation, moistening skin, and removing speckle. Can be used for treating pavor, insomnia, infantile convulsion, epilepsy, conjunctival congestion, nebula, unhealing sore and ulcer, and skin mottle.

Compared with the prior art, the invention adopts seven common white components in the traditional Chinese medicine: the seven medicines respectively belong to exterior-syndrome-relieving and cold-dispelling medicines, wind-dispelling and toxicity-dispelling medicines and heat-clearing and toxicity-dispelling medicines in traditional Chinese medicine classification, and have good antibacterial efficacy after being compounded, and also have the effects of inhibiting synthesis of melanin, whitening skin, removing freckles and the like. Especially, the contents of saponin and glucoside contained in the composition product can promote the stable dispersion of SOD and improve the antibacterial and skin-care effects.

Detailed Description

The following examples are given for the detailed implementation and specific operation of the present invention, but the scope of the present invention is not limited to the following examples.

Example 1

An antibacterial skin-care traditional Chinese medicine composition is a purified product extracted and purified from seven white formulas consisting of the following seven raw materials: 82 parts of angelica dahurica, 82 parts of bighead atractylodes rhizome, 82 parts of Japanese ampelopsis, 82 parts of white poria, 82 parts of cortex dictamni, 82 parts of rhizoma typhonii and 8 parts of pearl. The pearl is a white pearl crushed to 500-600 meshes.

(1) extraction of

Weighing 82 parts of angelica dahurica, 82 parts of bighead atractylodes rhizome, 82 parts of ampelopsis japonica, 82 parts of poria cocos, 82 parts of cortex dictamni and 82 parts of rhizoma typhonii by weight, adding deionized water for decocting, extracting for 2 times, wherein the water amount added for the first time is 8 times of the total weight of the six components, decocting for 1 hour, the water amount added for the second time is 8 times of the total weight of the six components, decocting for 0.5 hour, filtering, combining filtrates, and concentrating to 1g crude drug/mL at normal pressure for later use;

(2) purification of

Adding 2 times of ethanol into the water extract concentrated solution obtained in the step (1), stirring while adding, standing for 12 hours, separating supernatant, concentrating under reduced pressure until no ethanol smell exists, concentrating under reduced pressure at 60 ℃ to 1g crude drug/ml, adding 8 parts by weight of pearl, and mixing thoroughly.

Example 2

An antibacterial skin-care traditional Chinese medicine composition is a purified product extracted and purified from seven white formulas consisting of the following seven raw materials: 60 parts of angelica dahurica, 60 parts of bighead atractylodes rhizome, 60 parts of Japanese ampelopsis, 60 parts of white poria, 60 parts of cortex dictamni, 60 parts of rhizoma typhonii and 6 parts of pearl. The pearl is a white pearl crushed to 500-600 meshes.

(1) extraction of

Weighing six components of 60 parts by weight of angelica dahurica, 60 parts by weight of bighead atractylodes rhizome, 60 parts by weight of ampelopsis japonica, 60 parts by weight of poria cocos, 60 parts by weight of cortex dictamni and 60 parts by weight of rhizoma typhonii, adding deionized water for decocting for 2 times, wherein the water amount added for the first time is 6-10 times of the total weight of the six components, the decoction is carried out for 0.5 hour, the water amount added for the second time is 6 times of the total weight of the six components, the decoction is carried out for 0.1 hour, filtering, combining filtrates, and concentrating to 0.5g crude drug/mL under normal pressure for later use;

(2) purification of

Adding 1 weight time of ethanol into the water extract concentrate obtained in the step (1), stirring while adding, standing for 10 hours, separating supernatant, concentrating under reduced pressure until no alcohol smell exists, concentrating under reduced pressure at 50 ℃ to 0.5g crude drug/ml, adding 6 weight parts of pearl, and mixing thoroughly to obtain the final product.

Example 3

An antibacterial skin-care traditional Chinese medicine composition is a purified product extracted and purified from seven white formulas consisting of the following seven raw materials: 100 parts of angelica dahurica, 100 parts of bighead atractylodes rhizome, 100 parts of ampelopsis japonica, 100 parts of poria cocos, 100 parts of cortex dictamni, 100 parts of rhizoma typhonii and 10 parts of pearl. The pearl is a white pearl crushed to 500-600 meshes.

(1) extraction of

Weighing six components of 100 parts by weight of angelica dahurica, 100 parts by weight of bighead atractylodes rhizome, 100 parts by weight of ampelopsis japonica, 100 parts by weight of poria cocos, 100 parts by weight of cortex dictamni and 100 parts by weight of rhizoma typhonii, adding deionized water for decocting for 2 times, wherein the water amount added for the first time is 10 times of the total weight of the six components, decocting for 2 hours, the water amount added for the second time is 10 times of the total weight of the six components, decocting for 1 hour, filtering, combining filtrates, and concentrating to 2g crude drugs/mL at normal pressure for later use;

(2) purification of

Adding 3 times of ethanol into the water extract concentrated solution obtained in the step (1), stirring while adding, standing for 15 hours, separating supernatant, concentrating under reduced pressure until no ethanol smell exists, concentrating under reduced pressure at 80 ℃ to 2g crude drug/ml, adding 10 parts by weight of pearl, and mixing thoroughly.

Example 4

An antibacterial skin-care traditional Chinese medicine composition is a purified product extracted and purified from seven white formulas consisting of the following seven raw materials: 80 parts of angelica dahurica, 80 parts of bighead atractylodes rhizome, 80 parts of ampelopsis japonica, 80 parts of poria cocos, 80 parts of cortex dictamni, 80 parts of rhizoma typhonii and 8 parts of pearl. The pearl is a white pearl crushed to 500-600 meshes.

(1) extraction of

Weighing 80 parts of angelica dahurica, 80 parts of bighead atractylodes rhizome, 80 parts of ampelopsis japonica, 80 parts of poria cocos, 80 parts of cortex dictamni and 80 parts of rhizoma typhonii according to parts by weight, adding deionized water for decocting, extracting for 2 times, adding water 7 times of the total weight of the six components for the first time, decocting for 0.8 hour, adding water 7 times of the total weight of the six components for the second time, decocting for 0.6 hour, filtering, combining filtrates, and concentrating to 0.8g crude drug/mL at normal pressure for later use;

(2) purification of

Adding 1.5 times of ethanol into the water extract concentrate obtained in step (1), stirring while adding, standing for 11 hr, separating supernatant, concentrating under reduced pressure until no ethanol smell exists, concentrating under reduced pressure at 55 deg.C to 0.6g crude drug/ml, adding 8 weight parts of Margarita, and mixing thoroughly.

Example 5

An antibacterial skin-care traditional Chinese medicine composition is a purified product extracted and purified from seven white formulas consisting of the following seven raw materials: 85 parts of angelica dahurica, 85 parts of bighead atractylodes rhizome, 85 parts of Japanese ampelopsis, 85 parts of white poria, 85 parts of cortex dictamni, 85 parts of rhizoma typhonii and 9 parts of pearl. The pearl is a white pearl crushed to 500-600 meshes.

(1) extraction of

Weighing six components, namely 85 parts of angelica dahurica, 85 parts of bighead atractylodes rhizome, 85 parts of Japanese ampelopsis, 85 parts of poria cocos, 85 parts of cortex dictamni and 85 parts of rhizoma typhonii according to parts by weight, adding deionized water for decocting, extracting for 2 times, adding water for the first time which is 9 times of the total weight of the six components, decocting for 1.5 hours, adding water for the second time which is 9 times of the total weight of the six components, decocting for 0.6 hour, filtering, combining filtrates, and concentrating to 1.5g crude drug/mL at normal pressure for later use;

(2) purification of

Adding 2.5 times of ethanol into the water extract concentrate obtained in the step (1), stirring while adding, standing for 14 hours, separating supernatant, concentrating under reduced pressure until no ethanol smell exists, concentrating under reduced pressure at 75 ℃ to 1.5g crude drug/ml, adding 9 parts by weight of pearl, and mixing thoroughly to obtain the final product.

Control group: dermatitis treating medicine

The Chinese medicinal compositions prepared in examples 1 to 5 were subjected to the following test experiments to test their properties:

1. sterilizing and itching relieving performance

(1) Influence on itch caused by histamine phosphate

50 Kunming mice with weight of 20 +/-2 g and half each male and female were randomly divided into 5 groups, and the compositions obtained in examples 1 to 5 were tested by using models

The skin of the right hind paw of the mouse was polished with fine sandpaper, 0.02% histamine phosphate 0.1mL was dropped on the polished skin to the extent that the skin became red and bled without bleeding, and the number of times and duration of itching of the mouse within 15min were immediately observed and recorded. The spasmolytic indication is that the mouse returns to lick and gnaw the back and instep skin; the interval of the pruritus duration time is more than 3 seconds and is recorded as two pruritus, and the interval of the pruritus duration time is less than 3 seconds and is recorded as one pruritus. Immediately after molding, the composition prepared in each example was uniformly applied to the skin of the right hind paw of a mouse and gently rubbed for a moment, and the number and duration of itching of the mouse were observed and recorded within 15min, and the results are shown in table 1.

TABLE 1 antipruritic effect on histamine phosphate-induced pruritus

	Number of animals	Number of itching (times)	Duration (min)
				Example 1	10	10.2	100.1
Example 2	10	13.3	110.2
				Example 3	10	12.5	109.4
Example 4	10	11.4	105.5
				Example 5	10	11.8	103.7
Control group	10	12.6	105.3

The results show that the antipruritic cream has a remarkable antipruritic effect when being smeared on the skin of a pruritus model mouse, the effect is close to that of a positive drug control group, and the antipruritic effect is exact.

2. Sterilizing effect of medicated soap

Taking 10g of the composition obtained in each of examples 1-5, fully dissolving the composition in 50mL of double distilled water to serve as a test sample, immediately carrying out bacteriostasis and sterilization determination experiments, determining by a bacteriostasis zone size determination method and a gradient dilution sterilization method, wherein a strain is staphylococcus aureus, a blank control group adopts normal saline as the test sample, each group is carried out for three times, and the diameter of the bacteriostasis zone and the minimum sterilization concentration are determined, wherein the results are shown in Table 2:

TABLE 2 bacteriostatic effect

The result shows that the invention has excellent sterilization and bacteriostasis capability, and the minimum sterilization concentration is obviously lower than that of a control group.

3. Whitening and freckle removing effects

The test method comprises the following steps: any product (cosmetic or topical medicine) cannot be used 2 days before the test site is measured. Before the test, the testee needs to uniformly clean the face and wipe the face clean with dry facial tissue. After cleaning, the measurement area is marked on the face of the subject. Standing in room with room temperature of 25 deg.C and humidity of 45% for at least 30min before formal test, and standing in test state to keep relaxed.

Face marker 3X 3cm in experiment²The test area can simultaneously mark a plurality of areas, and the areas are spaced by 3 cm. In this example 6 areas were marked and the facial test areas before and after dosing were measured using a skin melanin and heme tester, each area being measured 5 times in parallel. The blank value of each test area was measured and then the Chinese medicinal composition of the present invention was applied. The melanin content of the skin in the test area was measured after 1 week and 2 weeks, respectively. The number of the tested persons is 30, and the average value is taken

Average melanin content	Before testing	After the film is adhered for one week	Insist on coating for two weeks
				Example 1	38％	28％	20％
Example 2	38％	32％	24％
				Example 3	38％	33％	25％
Example 4	38％	30％	22％
				Example 5	38％	29％	21％

It can be seen that the traditional Chinese medicine composition can obviously play a role in whitening and lightening spots when being smeared.

4. Determination of DPPH free radical eliminating capacity and SOD superoxide dismutase antioxidation capacity

Solution preparation:

weighing respectively:

VC 27.24, 49.41 and 99.11mg are added into 50ml of water;

examples 152.03, 99.35mg constant volume to 50ml water;

examples 250.49, 100.91mg constant volume to 50ml water;

examples 349.69, 104.2mg to 50ml water;

examples 450.71, 104.35mg were made up to 50ml of water.

Examples 548.51, 103.32mg were made up to 50ml of water.

Preparing a DPPH solution:

accurately weighing 4.91mg of DPPH into a 100mL volumetric flask, and using absolute ethyl alcohol to fix the volume to obtain 49.1mg/L DPPH mother liquor.

The determination method comprises the following steps:

2.0mL of each sample solution is respectively added into a 10mL colorimetric tube, and then 2.0mL of the PPH solution is parallelly added into each colorimetric tube. Control and blank groups were set simultaneously. And (4) lightly oscillating to fully and uniformly mix the mixture, and reacting the colorimetric tube in the dark for 30min to ensure that the medicine completely reacts with the free radicals. Measured at a wavelength of 517nm and the final A value is recorded. And (4) zero setting by taking absolute ethyl alcohol as a reference. The DPPH clearance (I) for each sample was calculated according to the following formula.

I/％＝〔1－(A_i－A_j)/A₀〕×100

In the formula A₀Absorbance of unpelled DPPH (2.0mL DPPH +2.0mL solvent), absorbance of Ai after reaction of sample with DPPH, and absorbance of Aj for sample blank (2.0mL95 ethanol +2.0mL sample). To reduce experimental error, 3-6 cuvettes were averaged for each sample.

Results of the experiment

The traditional Chinese medicine composition has good antioxidant effect.

The antibacterial skin-care traditional Chinese medicine combined seven-ingredient formula can be used in cosmetics or washing products, wherein the cosmetics comprise facial cleanser, cosmetic water, essence, cream, eye cream, facial mask, hand cream, body lotion and the like, and the washing products comprise perfumed soap, medicated soap, laundry detergent and the like.

Claims

1. The antibacterial skin-care traditional Chinese medicine composition is characterized by being a purified product extracted and purified from a seven-white formula consisting of the following seven raw materials, wherein the seven-white formula comprises the following components in parts by weight: 60-100 parts of angelica dahurica, 60-100 parts of bighead atractylodes rhizome, 60-100 parts of ampelopsis japonica, 60-100 parts of white poria, 60-100 parts of cortex dictamni, 60-100 parts of rhizoma typhonii and 6-10 parts of pearl.

2. The antibacterial skin-care traditional Chinese medicine composition according to claim 1, wherein the seven-ingredient prescription comprises: 80-85 parts of angelica dahurica, 80-85 parts of bighead atractylodes rhizome, 80-85 parts of ampelopsis japonica, 80-85 parts of white poria, 80-85 parts of cortex dictamni, 80-85 parts of rhizoma typhonii and 8-9 parts of pearl.

3. The antibacterial skin-care traditional Chinese medicine composition according to claim 1, wherein the pearl is a white pearl which is crushed to 500-600 meshes.

4. A method for preparing the antibacterial skin-care traditional Chinese medicine composition as claimed in any one of claims 1 to 3, which comprises the following steps:

(1) extraction of

(2) purification of

5. The method for preparing the antibacterial skin-care traditional Chinese medicine composition according to claim 4, wherein the water added in the step (1) is deionized water.

6. The preparation method of the antibacterial skin-care traditional Chinese medicine composition according to claim 4, wherein the composition product obtained in the step (2) contains 0.1-1 wt% of saponin and 0.1-1 wt% of glucoside.

7. The preparation method of the antibacterial skin-care traditional Chinese medicine composition according to claim 4, wherein the composition product obtained in the step (2) contains 0.1-1 wt% of SOD (superoxide dismutase) components, and the SOD components comprise three trace elements of manganese, copper and zinc.

8. The application of the antibacterial skin-care traditional Chinese medicine composition as claimed in any one of claims 1 to 3, wherein the traditional Chinese medicine composition is used in cosmetics or washing products, the cosmetics comprise facial cleanser, cosmetic water, essence, cream, eye cream, facial mask, hand cream and body milk, and the washing products comprise perfumed soap, medicated soap and laundry detergent.