KR20110063912A - A topical composition comprising the extract of platycodi radix, bupleuri radix and ginseng radix and the purified fractions therefrom inhibiting skin aging and wrinkle formation - Google Patents

Abstract

PURPOSE: A composition containing extract of Platycodon grandiflorum, red ginseng, and Bupleurum falcatum is provided to ensure antioxidation and collagen synthesis promotion. CONSTITUTION: An external use skin composition for anti-aging and anti-wrinkling contains 0.0001-20.0 weight% of saponin extract from Platycodon grandiflorum, red ginseng, and Bupleurum falcatum as an active ingredient. The composition is used in the form of external use skin ointment, skin softener, nutrition cream, massage cream, essence, pack, emulsion, or oil gel. The composition further contains oil, water, surfactant, moisturizing agent, low alcohol, thickening agent, chelating agent, coloring agent, or perfume.

Description

A topical composition comprising the extract of Platycodi Radix, Bupleuri Radix and Ginseng Radix and the purified fractions therefrom inhibiting skin aging and wrinkle formation}

The present invention provides an external skin pharmaceutical composition and cosmetic composition containing saponin obtained from Gilgyeong, red ginseng and Shiho exhibiting anti-aging and anti-wrinkle effect.

The human desire to have beautiful skin, that is, the desire to cultivate beauty and maintain youth, is human instinct since birth. To satisfy this, the term skin beauty was created and its history has long been established. In addition to beauty instincts, skin care has been focused on nine concepts of protecting skin from the outside.

Looking at the history of skin care, skin care in the age of the Joseon Dynasty solved the craving for white skin using wormwood and garlic, as shown in Dangun mythology, and the townspeople in the cold regions of the northeastern Korean peninsula avoided the cold by using pig oil. It is said to protect the skin and prevent frostbite. The Malgales are said to wash their skin with urine to make their skin whiter. In the Three Kingdoms period, maturity was developed with the development of paint making technology. In addition, in the Goryeo Dynasty, the face was decorated with white powder. In the Joseon Dynasty, the face was made using cucumbers and honey. There was a lot of interest in skin care so that recordings were taken.

Human skin is not only natural aging that ages with age, but also external aging caused by photoaging, which is caused by ultraviolet rays contained in sunlight. A representative cause is oxidative damage.

Skin aging processes by free radicals include lipid peroxidation, protein oxidation, DNA oxidation, melanin production, connective tissue breakdown and abnormal crosslinking. Most of these reactions are radical reactions and proceed through automatic oxidation. Automated oxidation is a chain reaction, which causes many biological components to undergo oxidative damage during this process. Therefore, the automatic oxidation reaction should be blocked as soon as possible. Antioxidants are primarily responsible for blocking the automatic oxidation in vivo.

Collagen is a major matrix protein produced in skin fibroblasts and is present in extracellular keratin and is an important protein that accounts for 30% of the total weight of biological proteins. The main functions are known as mechanical firmness of skin, resistance of connective tissue and binding of tissue, support of cell adhesion, induction of cell division and differentiation. Collagen is also destroyed by ultraviolet light exposure, which is an external cause of skin aging, and the change caused by ultraviolet light is known to be proportional to the accumulation of time exposed to ultraviolet light. Ultraviolet rays accumulate elastic fibrous material in the dermis of the skin and denature collagen fibers, causing wrinkles on the skin and reducing elasticity.

The present inventors came to pay attention to saponins during the search for a drug that inhibits skin aging against ultraviolet rays from a safe and low-toxic herbal medicine, and the saponins of Gilkyung, red ginseng and Siho contained in large amounts promote collagen synthesis with excellent antioxidant power. The present invention was completed by confirming that wrinkle improvement and skin aging were inhibited.

An object of the present invention is to provide an external skin pharmaceutical composition and cosmetic composition containing saponin obtained from Gilgyeong, red ginseng, and Shiho, which have an anti-aging and anti-wrinkle effect.

The present invention provides an external skin pharmaceutical composition and cosmetic composition containing saponin obtained from Gilgyeong, red ginseng and Shiho exhibiting anti-aging and anti-wrinkle effect.

To this end, in the present invention, saponin components were extracted from Gilgyeong, red ginseng and Shiho. Specifically, Gilkyung, red ginseng, and Siho were sequentially extracted using aqueous and organic solvents to obtain purified saponins. As a method for purifying saponin from Gilkyung, red ginseng and Shiho, all conventional extraction methods known to those skilled in the art are available.

      Gil-gil (은) is the root of the perennial herbaceous Platycodon grandiflorum belonging to the family of the Campanula, about 10 kinds of saponins were separated. The main saponins are platycodin D, which is about 0.2% of the dry path. In oriental medicine, Hwadam is known to have the effect of haedamhaehwa, yiingaeum (배 音), pear farming (옹) and modern pharmacological effects such as expectoration, Jinhae, anti-inflammatory, analgesic do.

Red ginseng is made by steaming dried roots of ginseng, Panax ginseng, two or three times. There are a lot of Ginsenoside ginseng and red ginseng saponin. In oriental medicine, the effects of Daebowon period, Bobi-ik lung, Saengjinjigal and Anshin-ikji are known. In modern pharmacology, central nervous system regulation and immune enhancement And increase blood circulation.

Shiho (柴胡) is a dried perennial herbaceous herbaceous root (Bupleurum falcatum) or its roots. It contains mainly saponins, including saikosaponin A, B, C, and D. In oriental medicine, there are effects such as reconciliation, fever, small liver and yangyanggi, and modern pharmacology has antipyretic, soothing, analgesic and antitussive effects. .

The saponin extract obtained as described above preferably contains 0.0001 to 20.0% by weight.

There is no restriction | limiting in particular in the kind of external preparation for skin to which this invention can be applied, and all the usual external formulations can be applied. For example, the composition may be prepared in the form of external skin ointment, softening longevity, nourishing longevity, nutrition cream, massage cream, essence, pack, emulsion, oil gel and the like. Here, the skin external ointment is prepared to contain 50.0 to 97.0% by weight of petrolatum, and 0.1 to 5.0% by weight of polyoxyethylene oleyl-ether phosphate in addition to the active ingredient of the compound represented in Example 1, the softening longevity is propylene glycol, It is prepared to contain 1.0 to 10.0% by weight of polyhydric alcohols such as glycerin and 0.05 to 2.0% by weight of surfactants such as polyethylene oleyl ether and polyoxyethylene hydrogenated castor oil. Nutrients and nourishing creams contain 5.0 to 20.0% by weight of oils such as squalane, petrolatum and octyldodecanol, and 3.0 to 15.0% by weight of wax components such as cetanol, stearyl alcohol and beeswax in addition to the saponin composition. It contains 5.0 to 30.0% by weight of polyhydric alcohols such as glycerin and propylene glycol. Massage cream is prepared by containing 30.0 to 70.0% by weight of oil, such as liquid paraffin, petrolatum, isononylisononanoate in addition to the active ingredient of the saponin composition represented in Example 1, the pack is 5.0 to 20.0% by weight of polyvinyl alcohol It is prepared as a wash off pack containing 5.0 to 30.0% by weight of pigments such as kaolin, talc, zinc oxide and titanium dioxide in a peel off pack or a general emulsified external preparation containing%.

On the other hand, the skin external preparation for improving skin aging and wrinkle improvement containing the saponin composition of the present invention is a common ingredient, for example, oil, water, surfactants, moisturizers, lower alcohols, thickeners, chelates that are formulated in general external skin preparations. It is possible to apply | blend an agent, a pigment | dye, preservative, a fragrance | flavor, etc. as needed.

Hereinafter, preferred embodiments of the present invention will be described in order to facilitate understanding of the present invention. However, the following examples are provided only to more easily understand the present invention, and the present invention is not limited to the following examples.

Saponin extracts obtained from Gilgyeong, Red Ginseng and Siho of the present invention show excellent antioxidant and collagen biosynthetic effects, and can be used as an external skin pharmaceutical composition and cosmetic composition for anti-aging and wrinkle improvement.

Example  1. Extraction of Saponin

100 g and 500 mL of 70% alcohol were cooled to reflux for 3 hours, respectively. The filtrate was concentrated, and 120 mL of ether and 200 mL of distilled water were added to a separatory funnel in 40 mL of the concentrate, followed by phase separation for 4 hours. Then, 120 mL of butanol was added to the lower aqueous layer, followed by phase separation for 4 hours. The upper butanol layer was washed five times with 100 mL of distilled water, and the washed butanol layer was concentrated and distilled water was added to completely remove butanol. Red ginseng and Shiho were also extracted in the same way.

Experimental Example  1. Antioxidant effect measurement

In order to determine the antioxidant effect of the herbal extracts prepared in Examples 1, 2 and 3, a free radical scavenging activity test was performed. Free radical scavenging activity test was performed by 1,1-Diphenyl-2-Pycryl-Hydrazyl (DPPH) method (Blois, M.S. Nature 181, 1190 (1958)). DPPH is a relatively stable free radical which, when present in the radical state, exhibits maximum absorption at 517 nm and loses its absorbance when erased. DPPH was used by Sigma Co., Ltd. and dissolved in methyl alcohol at a concentration of 0.15 mM. First, the medicinal herb extracts prepared in Experimental Examples 1, 2 and 3 were prepared for each concentration, 2 mL each was added and mixed, and the mixture was left at room temperature for 10 minutes, and then absorbance was measured at 517 nm. At this time, the control group was used as the case using purified water. Thereafter, the free radical scavenging ratio was calculated using the absorbance according to Equation 1 below, and the results are shown in Table 1 below. The free radical scavenging rate of Table 1 was determined by five repeated tests.

Equation 1. Free radical elimination rate (%)

= {100- (absorbance of experimental group-blank / absorbance in control)} x 100

Free radical scavenging rate extract Concentration (ug / mL) Free radical scavenging rate (%) Gilkyung Saponin Extract 0.1 0.7 One 1.2 10 10.1 100 15.5 1,000 20.1 10,000 22.4 Red Ginseng Saponin Extract 0.1 2.7 One 3.3 10 22.1 100 27.5 1,000 38.1 10,000 48.7 Shiho Saponin Extract 0.1 0.7 One 4.3 10 15.1 100 27.5 1,000 45.1 10,000 54.8 Gilkyung, Shiho, Red Ginseng Mixed Saponin Extract 0.1 5.7 One 10.4 10 37.5 100 54.4 1,000 66.9 10,000 80.4

As can be seen from the above results, the anti-aging effect of the mixed saponin extract mixed with the same amount was higher than that of each saponin extract of Gilkyung, red ginseng, and Siho.

It was found that the efficacy is excellent.

Experimental Example  2. Collagen Synthesis Effect Test

The saponin extract obtained in Example 1 was added to the culture solution of human-derived fibroblasts to test collagen synthesis promoting effect at the cellular level. Biosynthetic collagen measurements were quantified using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immuno Assay KIT).

The concentration of the saponin extract obtained in Example 1 was added to the culture medium of human-derived fibroblasts so that the final concentration was as follows. It was measured at 450 nm using a photometer. Collagen biosynthesis was calculated as the relative synthetic performance of the control (no addition) and the results are summarized in Table 2.

Collagen Biosynthesis Type of sample Collagen Synthesis Enhancement Effect (%) Control group (serum free medium) 100 Street slopin extract 0.1% 111.3 0.01% 106.9 Shisa Saponin Extract 0.1% 109.3 0.01% 102.2 Red Ginseng Saponin Extract 0.1% 110.6 0.01% 104.3 Gilkyung, Siho, Red Ginseng Mixed Saponin Extract 0.1% 122.3 0.01% 110.5

Experimental Example  3. Cytotoxicity Assessment

Performing the MTT test (Mossman TJ Immunol. Meth. 55-63 (1983)) by culturing the V79-4 cells (Chinese hamster, lung tissue fibroblasts continuous cell line) for the saponin extract of Example 1 The cytotoxicity was tested and the results are shown in Table 3 below. At this time, SLS was used as a control.

 Cytotoxicity Assessment sample IC50 (%, w / v) SLS 0.005 Squalane 0.05 Example 1 10.00

As can be seen in Table 3, IC ₅₀ values of Gilkyung, red ginseng, and Shiho saponin extracts were 10%, indicating that cytotoxicity was two times lower than that of SLS, indicating excellent safety.

Claims (4)

Skin external composition for anti-aging and wrinkle improvement containing saponin extract obtained from single or two or more herbal medicines selected from the group consisting of Gilkyung, red ginseng and Siho as an active ingredient The method of claim 1, Skin external composition having anti-wrinkle and improvement effect through excellent antioxidant effect and collagen synthesis effect The method of claim 1, The external composition for skin anti-aging and wrinkle improvement, characterized in that the saponin composition is a skin external ointment, softening lotion, nourishing longevity, nutrition cream, massage cream, essence, pack, emulsion or oil gel formulation. The method of claim 1, The saponin composition further comprises at least one additive selected from the group consisting of oil, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives and fragrances. Improvement composition for external skin