JP2009102378A - Pharmaceutical agent for pruritus, rough skin, sensitive skin and whitening by suppressing production/release of stem cell factor - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for screening an active ingredient ameliorating skin pruritus, rough skin and sensitive skin or exerting whitening effect, and various pharmaceutical agents containing the active ingredient. <P>SOLUTION: The external preparation for the skin for suppressing production and/or release of stem cell factor (SCF) contains active ingredients of herbal medicine that ameliorates skin pruritus, rough skin and sensitive skin or exerts whitening effect by suppressing production and/or release of SCF. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a screening method for an active ingredient that improves skin pruritus, rough skin and sensitive skin, or exhibits a whitening effect by suppressing the production and / or release of stem cell factor (hereinafter “SCF”), and the active ingredient It relates to a drug for pruritus, rough skin, sensitive skin and / or whitening.

  Conventionally, various therapeutic agents, external preparations for skin, cosmetics and the like have been used as those having skin pruritus, rough skin, sensitive skin improvement effect and whitening effect. In conventional medicines and cosmetics, etc. that exhibit the effects of improving skin pruritus, rough skin, and sensitive skin, various active animals and plants that have anti-inflammatory or high moisturizing effects in addition to anti-inflammatory agents, amino acids, polysaccharides and lipids as active ingredients Extracts have been used because of their ability to prevent itching, inflammation, and loss of water in the stratum corneum. In addition, in conventional drugs and cosmetics that exhibit a whitening effect, L-ascorbic acid, glutathione, kojic acid, cysteine, hydroquinone, placenta extract and the like as active ingredients suppress the production of melanin, freckles, etc. Has been used because it is excellent in preventing. However, in any case, the effect is not always sufficient, and development of a superior drug is desired.

  It is known that the expression of SCF (also known as kit ligand [KL] or mast cell growth factor [MCF]) is enhanced at the site of staining, and that the expression of SCF is enhanced by ultraviolet irradiation (LH Kligman et al. al., Photochem. Photobiol. Vol.63, No.2 (1996) pp.123-127). SCF is a protein produced in keratinocytes, fibroblasts, vascular endothelial cells and the like. SCF functions include proliferation of undifferentiated hematopoietic stem cells, promotion of germ cell differentiation, promotion of mast cell growth, pigment cell growth promotion, etc. (Bio Science Terminology Library Cytoikaine / Growth Factor Yodosha ( 1995) Kozo Miyazono, Kazuo Kashimura). It is known that SCF includes a membrane-bound type (SCF-2) and a secreted type (SCF-1) that is released from a membrane cleaved by the action of a proteolytic enzyme. SCF-2 binds to the SCF receptor of the pigment cell while bound to keratinocytes and activates proliferation of the pigment cell, and SCF-1 is cleaved at the cleavage site and released from the cell membrane. It binds to the SCF receptor of cells and activates proliferation of pigment cells, and also leads to proliferation activation and degranulation of mast cells (T. Kunisada et al., J. Exp. Med., Vol. 187, No. 10, (1998) pp.1565-1573). Abnormal production of SCF leads to abnormal proliferation of pigment cells, increases melanin production, and causes spots, freckles, dullness and the like. It also leads to abnormal growth and abnormal degranulation of mast cells, and enhances the release of chemical mediators such as histamine, serotonin, and LTB4 (J. Grabbe et el., Arch Dermatol Res (1984) 287: 78-84) Cause itching, rough skin, and sensitive skin.

  Therefore, if it is possible to effectively suppress the production and release of SCF, which is considered to be a direct cause of such pruritus, rough skin, sensitive skin, stains, freckles, etc., completely new and more effective pruritus and rough skin that have never existed before It is believed that this will lead to the provision of drugs for sensitive skin and / or whitening. However, in the prior art, neither a substance or a crude drug having such SCF production and release inhibiting activity nor an effective screening method for such a substance or herbal medicine is known. T. Kunisada et al (supra) are examining the effect of SCF on mast cells and pigment cells by animal experiments using transgenic mice. However, since the use of such animal experiments is time consuming and expensive, it is not suitable for screening a wide variety of herbal medicines and drugs, and more easily and inexpensively screens active ingredients or herbal medicines that have SCF production and release inhibiting activity. A possible method was desired.

  Surprisingly, the present inventor has found that the production or release of SCF can be promoted by giving stimulation such as drying to human epidermal keratinocytes, so that the production and / or release of SCF is effective. We have succeeded in efficiently screening active ingredients that can be suppressed.

That is, in the first aspect, the present invention is a method for screening an active ingredient that exhibits an effect of improving pruritus, rough skin or sensitive skin or whitening effect by suppressing SCF production and / or release, wherein In the method of contacting a cell to be expressed with a test ingredient, measuring the amount of SCF produced and / or released by the cell, and selecting a test ingredient that reduces the production and / or release of SCF as the active ingredient, Provided is a method characterized by stimulating the SCF-expressing cells to promote SCF production and / or release.
In one aspect, the stimulus is a dry stimulus, an ultraviolet irradiation stimulus or a drug stimulus, preferably a dry stimulus.
In another aspect, the screening method comprises contacting the SCF-expressing cell with the test component, then stimulating the cell, and then measuring the amount of SCF produced and / or released by the cell. This is a method for selecting ingredients.

  In a second embodiment, the present invention is rose extract water, tea extract, hop extract, hawthorn extract, azuki bean powder, birch extract, cinnamon extract, clove extract, arnica extract, button extract, bodaiju, chlorella extract, roman chamomile extract, black tea extract , Eucalyptus extract, Sojutsu extract powder, Peony extract powder, Oolong tea extract powder, Onionis extract, Asenyaku extract, Grape leaf extract, Bow extract, Mulberry extract, Parietalia extract, Anchovy extract, Stevia extract, Cypress, Cypress root extract, Soybean extract, Provided is a skin external preparation for SCF production and / or inhibition comprising one or more selected from the group consisting of scorpion extract, bonnet extract, altea extract, hypericum extract and mugwort extract.

In a third aspect, the present invention provides a skin external preparation for pruritus comprising a component that suppresses SCF production and / or release as an effective ingredient for pruritus improvement.
In one aspect, the active ingredient for improving pruritus is hop extract, hawthorn extract, azuki bean powder, clove extract, roman chamomile extract, black tea extract, eucalyptus extract, sojutsu extract powder, peanut extract powder, oolong tea extract powder, onionis extract, boufu extract Or one or more selected from the group consisting of parietalia extract, anthracnose extract, stevia extract, ginger root extract, scallop extract, savonso extract, altea extract and hypericum extract.

In a fourth aspect, the present invention provides a skin roughening preparation for preventing rough skin, which contains a component that suppresses the production and / or release of SCF as a rough skin preventing active ingredient.
In one aspect, the active ingredient for preventing rough skin is hawthorn extract, Sojutsu extract powder, peanut extract powder, onionis extract, mulberry extract, parietalia extract, Ansokkou extract, stevia extract, cypress, ginger root extract, Kajikazura, Sabonso extract and Altea One or more selected from the group consisting of extracts.

In a fifth aspect, the present invention provides an external preparation for sensitive skin, which contains an ingredient that suppresses SCF production and / or release as an active ingredient for improving sensitive skin.
In one aspect, the active ingredient for improving sensitive skin is rose extract rose water, tea extract, hop extract, hawthorn extract, azuki bean powder, birch extract, cinnamon extract, clove extract, chlorella extract, black tea extract, eucalyptus extract, powdery extract, peanut Extract powder, oolong tea extract powder, onionis extract, asenyaku extract, grape leaf extract, bowfish extract, mulberry extract, parietalia extract, anthracite extract, stevia extract, cypress, ginger root extract, soybean extract, scorpion extract, savons extract, altea extract, One or more selected from the group consisting of hypericum extract and mugwort extract.

In a sixth aspect, the present invention provides a skin whitening external preparation containing a component that suppresses the production and / or release of SCF as a whitening active ingredient.
In one aspect, the whitening active ingredient is one or more selected from the group consisting of rose extract rose water, onionis extract, acacia catechu extract, parietalia extract, anthracite extract, scorpion extract, chlorella extract, and eucalyptus extract.

  In a seventh aspect, the present invention applies an active ingredient that exerts an effect of improving pruritus, rough skin or sensitive skin or whitening effect by inhibiting the production and / or release of SCF to the epidermis of humans or mammals. Providing an improvement or whitening method for pruritus, rough skin or sensitive skin comprising.

In an eighth aspect, the present invention provides a drug containing a component that suppresses SCF expressed in keratinocytes or on a cell membrane by UV stimulation.
In one aspect, the ingredients include rose extract water, tea extract, hop extract, hawthorn extract, azuki bean powder, birch extract, cinnamon extract, clove extract, arnica extract, button extract, bodaiju, chlorella extract, roman chamomile extract, black tea extract, Eucalyptus extract, Sojutsu extract powder, Sandalwood extract powder, Oolong tea extract powder, Onionis extract, Asenyaku extract, Grape leaf extract, Bow-fu extract, Mulberry extract, Parietalia extract, Anchovy extract, Stevia extract, Cypress, Ginger root extract, Soybean extract, Kajikazura , One or more selected from the group consisting of scorpion extract, altea extract, hypericum extract and mugwort extract.
In another aspect, the ingredient is azuki bean powder, arnica extract, onionis extract or anthracnose extract.

  According to the present invention, herbal medicines could be efficiently screened for SCF production / release.

  The present invention is described in detail below.

  The present invention provides a method for screening an active ingredient that exhibits an improvement effect or a whitening effect on pruritus, rough skin or sensitive skin by suppressing the production and / or release of SCF. In this screening method, a test ingredient that reduces the amount of SCF produced and / or released by contacting SCF-expressing cells with a test ingredient and measuring the amount of SCF produced and / or released by the cells. In this case, the SCF-expressing cells are stimulated to promote SCF production and / or release.

  The present inventor has found that when skin cells such as human keratinocytes are stimulated such as dryness, the production and / or release of SCF in the cells is promoted. Therefore, by giving such stimulation to the cells, it is possible to induce abnormal expression of SCF, which leads to the onset of pruritus, rough skin, sensitive skin and spots, freckles, and the like. Utilizing this fact, the present inventor can suppress the expression of SCF by applying a stimulus that promotes the production and / or release of SCF when a test component that suppresses the production and / or release of SCF is allowed to act on cells. The present inventors succeeded in efficiently screening active ingredients that can enhance and effectively suppress the production and / or release of SCF. Examples of such a stimulus include stress such as ultraviolet irradiation stimulus, heat stimulus (heating and cooling), drug stimulus (for example, forskolin, theophylline), osmotic stimulus, and oxidative stimulus in addition to dry stimulus.

  The stimulation of the cell in the screening method according to the present invention may be before, simultaneously with, or after allowing the test component to act on the cell. Preferably, the cell is stimulated after the test component is allowed to act on the cell.

Specifically, the screening method can be performed as follows, for example.
(1) SCF producing cells are cultured using an appropriate culture solution (for example, about 25 to 37 ° C., preferably about 37 ° C. for several hours to several days, preferably about 72 hours).
(2) The test component is diluted to a predetermined concentration (eg, 0.0001% -0.01%) with an appropriate cell culture medium.
(3) The cell culture solution of (1) is replaced with the diluted test component and incubated (for example, about 25 to 37 ° C., preferably about 37 ° C. for several hours to several days, preferably about 24 hours). At that time, as a control, a cell in which a medium not containing the test component is similarly acted is prepared.
(4) Next, a stimulus is given to the cells to which the test component has been applied. If the stimulus is a dry stimulus, the cell culture supernatant is removed and incubated under dry conditions. At this time, optionally, a cell not subjected to the stimulation is also prepared as a control.
(5) A medium is added to the stimulated cells and incubated (for example, about 25 to 37 ° C., preferably about 37 ° C. for about 30 minutes to 4 hours, preferably about 2 hours).
(6) Collect the supernatant of the cells, measure the amount of SCF, and select components that suppress SCF production and / or release.

  SCF production / release is suppressed when the medium alone is added as 100%, and when the test component is added, the SCF production is reduced by about 25%. However, the test component should be added in a concentration range that does not cause significant cytotoxicity (for example, a decrease in respiratory activity up to about 75%).

  The SCF producing cells to be used may be, for example, keratinocytes, fibroblasts, vascular endothelial cells derived from humans or other mammals such as rats, mice, rabbits and the like. Preferably, human keratinocytes are used.

As described above, the stimulation includes stress such as drying stimulation, ultraviolet irradiation stimulation, thermal stimulation (heating and cooling), drug stimulation (for example, forskolin and theophylline), osmotic stimulation, and oxidative stimulation. For drying stimulation, for example the cells CO ₂ about 1-5% in a CO ₂ incubator, humidity of about 0 to 100%, temperature 25 to 37 appropriate time by removing the medium in an atmosphere at ° C., such as about 15 minutes The cells may be given a dry stimulus by standing for -6 hours, preferably about 1 hour. In the case of ultraviolet irradiation stimulation, for example, the cells may be stimulated by irradiating the cells with ultraviolet rays at about 290 to 320 nm and 10 to 60 mJ / cm ² . In the case of heat stimulation, for example, the cells are placed in a CO ₂ incubator at about 1 to 5% CO ₂ , about 0 to 100% humidity, a temperature of 4 to 25 ° C. or 37 to 42 ° C. for an appropriate time, for example, about 5 minutes to 6 hours. Preferably, the cells may be stimulated by leaving them for about 1 hour.

  The screening method of the present invention is preferably performed in vitro, but can also be performed in vivo.

Measurement of SCF In the present invention, the measurement of SCF produced and / or released by SCF-expressing cells is preferably performed by measuring SCF released by the cells into the culture solution or SCF contained in the disrupted cells or cell membrane fractions. Perform by qualitative or quantitative measurement. The SCF to be measured may be secreted (SCF-1) released from the cell membrane and released into the cell culture medium, or membrane-bound (SCF-2) contained in the cell membrane fraction. SCF can be measured by methods well known in the art, for example, immunoassay methods such as ELISA using an enzyme label, RIA using a radioactive label, and the change in absorbance of turbidity caused by the reaction between an antibody and an antigen. Various methods such as an immunoturbidimetric method for quantification, a latex agglutination method for measuring the amount of antigen based on the degree of agglutination caused by a reaction between an antigen and antibody-sensitized latex beads or antibody-sensitized erythrocytes, and a erythrocyte agglutination method can be mentioned. Examples of immunoassay methods include competitive methods and sandwich methods. In addition, electrophoresis, isoelectric focusing, chromatography, such as gel filtration chromatography, ion exchange chromatography, reverse phase chromatography, high performance liquid chromatography, Western blotting, etc. can do. In the present invention, preferably, the SCF is measured by ELISA.

  The antibody specific for SFC used in the immunoassay method may be a monoclonal antibody or a polyclonal antibody. Monoclonal antibodies are particularly preferred. The production of monoclonal and polyclonal antibodies is well known to those skilled in the art.

The present inventor has found that according to the screening method according to the present invention, the following crude drugs can reduce the amount of SCF derived from SCF-producing cells:
Rose extract rose water, tea extract, hop extract, hawthorn extract, azuki bean powder, birch extract, cinnamon extract, clove extract, arnica extract, button extract, bodaiju, chlorella extract, roman chamomile extract, black tea extract, eucalyptus extract, sweet potato extract powder, peanut Extract powder, oolong tea extract powder, onionis extract, asenyaku extract, grape leaf extract, bowfish extract, mulberry extract, parietalia extract, anthracite extract, stevia extract, cypress, ginger root extract, soybean extract, scorpion extract, savons extract, altea extract, Hypericum extract, mugwort extract. For details of such crude drugs, please refer to the 4th edition of Japan General Cosmetics Raw Materials Collection (Pharmaceutical Daily).

  The above crude drug extract is extracted from the raw material plant by a conventional method, and is not limited to the extraction method. For example, in the case of a clove extract, Japanese pharmacopoeia clove is dried and shredded into 10 kg, 50 v It can be obtained by adding / v% ethanol (prepared with cosmetic raw material standards, absolute ethanol and the same purified water), soaking for 24 hours, pressing and separating to obtain an extract.

The pruritus, rough skin and sensitive skin in the present invention refer to those induced by binding of SCF released by cells to the SCF receptor of mast cells. Pruritus means itching of the skin and scalp in a broad sense, and examples include pruritus due to dryness, atopic dermatitis, senile xeroderma. Rough skin means a state of skin deterioration in a broad sense, and includes rough skin caused by scratching due to pruritus and worsening deterioration of atopic dermatitis, rough skin due to drying, and the like. Sensitive skin means skin with increased irritation, non-specific physicochemical stimulation from the outside world, such as scratching, heat, sweating, sunlight, clothes, deposits, etc., and mental stress stimulation It refers to the condition of the skin that reacts sensitively.
The whitening effect in the present invention is to improve stains, freckles, dullness, etc. caused by excessive production of melanin induced by binding of SCF released by cells due to sunburn, drying or other causes to the SCF receptor of pigment cells. It means to do.

  The agent for pruritus, rough skin, sensitive skin and whitening of the present invention is usually used by containing the SCF production / release inhibiting active ingredient in an aqueous solvent such as water or ethanol. The blending amount of the SCF production / release inhibiting component of the present invention is not particularly limited, but is preferably in the range of about 0.00001 to 0.01% by weight, particularly about 0.0001 to 0.005% by weight in terms of solid content. In addition, when preparing the chemical | medical agent which concerns on this invention as a bath agent, since it is normally diluted about 100 to 1000 times at the time of use, it is preferable that a mixing | blending is prescribed by the high density | concentration which considered it. As said aqueous solvent, a lower alcohol is preferable and content of a lower alcohol is 20 to 80 weight% in a chemical | medical agent of this invention, More preferably, it is 40 to 60 weight%.

  In addition, the agent for pruritus, rough skin, sensitive skin, and whitening of the present invention, in addition to the above essential components, components that are usually used in external preparations for skin such as cosmetics and pharmaceuticals, such as other whitening agents, moisturizing agents, and antioxidants. An agent, an oily component, an ultraviolet absorber, a surfactant, a thickener, an alcohol, a powder component, a colorant, an aqueous component, water, various skin nutrients, and the like can be appropriately blended as necessary.

  Other metal sequestering agents such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, gluconic acid, caffeine, tannin, verapamil, tranexamic acid and their derivatives according to the use of the drug Licorice extract, grabrizine, hot water extract of karin fruit, various herbal medicines, tocopherol acetate, glycyrrhizic acid and its derivatives or salts thereof, vitamin C, magnesium ascorbate phosphate, glucoside ascorbate, arbutin, kouji Other whitening agents such as acids, sugars such as glucose, fructose, mannose, sucrose, and trehalose, vitamin A such as retinoic acid, retinol, retinol acetate, and retinol palmitate can also be appropriately blended.

  The drug for pruritus, rough skin, sensitive skin and whitening of the present invention is adapted to its use, for example, external preparations such as cosmetics, pharmaceuticals, quasi-drugs, such as lotions, creams, emulsions, lotions, packs, bath preparations, Any ointment, hair lotion, hair nick, hair liquid, shampoo, rinse, hair nourishing and hair restoring agent, and the like used in conventional skin external preparations may be used, and the dosage form is not particularly limited.

  Next, the present invention will be described in more detail with reference to examples.

Experimental example 1
Measurement of SCF released from keratinocyte membranes by dry stimulation and screening of inhibitory drugs GIBCO). Cells were seeded at 1 × 10 ⁵ cells / mL in a 12-well microplate and cultured at about 37 ° C. for about 72 hours until confluent.
Each herbal extract shown in FIGS. 1 and 2 extracted with water or ethanol was dissolved in 70% ethanol so as to be 2% w / v.
The test crude drug extract was added to the culture solution to a final concentration of 0.005% w / v and incubated at 37 ° C. for 24 hours. As a control, ethanol (EtOH) alone was also incubated. In the last 2 hours, in order to examine the cell activity, that is, to examine the effect of the cytotoxic action of the test crude drug extract, alamarBlue ™ (Biosource International) was added to 10%, and the fluorescence intensity of the supernatant was added. (excitation 560 nm, emission 590 nm) was measured.
As a drying stimulus, the supernatant was completely removed and left in a CO ₂ incubator for 1 hour.
The medium was then added and the supernatant was collected 2 hours later. The amount of SCF in the supernatant was quantified with a commercially available ELISA measurement kit (R & D Systems).
The SCF amount inhibitory action of the test crude drug extract was evaluated using a control without drying stimulation as a control.
At the same time, the cytotoxicity of the test crude drug extract was evaluated using the reduction amount of alamarBlue ™ as an index.

  The results are shown in FIGS. FIGS. 1 and 2 (a) show the reduction amount of alamarBlue TM when each herbal extract is added, and (b) shows the amount of free SCF in the medium of the cells to which each herbal extract has been added and given dry stimulation. Shows the value obtained by subtracting the free SCF in the medium of the cells to which each herbal extract was added and no dry stimulation was given. As is apparent from the results of FIGS. 1 and 2, it was found that various herbal extracts can significantly reduce the amount of SCF in the culture compared to ethanol, and thus can effectively suppress the production and / or release of SCF. .

Experimental example 2
Increased expression of SCF on keratinocyte membrane by various stimuli
After seeding 1.5 million keratinocytes in a 10 cm plate and culturing for 72 hours, (1) replace the medium with PBS (-), irradiate with UVB at 20 mJ / cm ² and immediately add PBS (-) to the medium. Cells were stimulated by exchange or (2) forskolin added or (3) theopholin added. After culturing for 24 hours, the cells were collected and diffused in 200 μl of 50 mM phosphate buffer (pH 7.8) + protease inhibitor solution, and then disrupted at 4 ° C. for 30 seconds and 5 times for 30 seconds. This was centrifuged at 10,000 g for 20 minutes at 4 ° C., and the supernatant was further centrifuged at 100,000 g for 60 minutes at 4 ° C. The obtained pellet was dissolved in 100 μl of 25 mM phosphate buffer (pH 6.8) + 0.1% Triton-X100 solution to obtain a membrane fraction protein extract. The amount of protein in this solution was measured by a conventional method, and the amount of SCF was further measured to obtain a value of SCF amount / protein amount.

  The results are shown in FIGS. As is apparent from the results in the figure, it was revealed that when cells were stimulated with ultraviolet light or drug stimuli such as forskolin or theophylline, the expression of SCF in the cell or on the cell membrane was enhanced.

Experimental example 3
Measurement of SCF expressed on keratinocyte membrane by UV stimulation and screening of inhibitory drugs
After seeding 1.5 million keratinocytes in a 10 cm plate and culturing for 72 hours, the medium is replaced with PBS (−), UVB is irradiated at 20 mJ / cm ² , and PBS (−) is immediately replaced with the medium. The crude drug to be screened was added, and the cells were collected after 24 hours of culture. After diffusing the cells in 200 μl of 50 mM phosphate buffer (pH 7.8) + protease inhibitor solution, 5 times for 30 seconds using an ultrasonic crusher Cells were disrupted at 4 ° C. This was centrifuged at 10,000 g for 20 minutes at 4 ° C., and the supernatant was further centrifuged at 100,000 g for 60 minutes at 4 ° C. The obtained pellet was dissolved in 100 μl of 25 mM phosphate buffer (pH 6.8) + 0.1% Triton-X100 solution to obtain a membrane fraction protein extract. The amount of protein in this solution was measured by a conventional method, and the amount of SCF was further measured to obtain a value of SCF amount / protein amount. With respect to this value, a candidate herbal medicine that is reduced to 25% or less in the target drug addition group as compared with the group to which only the solvent is added is defined as the inhibitor drug.

  The result is shown in FIG. As is clear from the results of FIG. 5, it was found that the cells treated with various herbal extracts were significantly suppressed in the expression of SCF as compared with the cells treated only with ultraviolet irradiation.

Below, the formulation example of the crude drug extract containing chemical | medical agent by this invention of various dosage forms is enumerated.
Example 1
Body cream
Compounding amount (parts by weight)
────────────────────────────────────
Methylpolysiloxane 3
Decamethylcyclopentasiloxane 13
Octamethylcyclotetrasiloxane 12
Polyoxyethylene / methylpolysiloxane copolymer 1
Ethanol 2
Isopropanol 1
Glycerin 3
Dipropylene glycol 5
Polyethylene glycol 6000 5
Sodium metaphosphate 0.05
DL-α-Tocopherol 2-L-Ascorbic acid phosphate diester potassium 0.1
DL-α-tocopherol acetate 0.1
Caffeine 0.1
Fennel extract 0.1
Hamelis Extract 0.1
Carrot extract 0.1
Sowjutsu extract powder 0.005
L-menthol appropriate amount p-hydroxybenzoate appropriate amount edetate trisodium 0.05
Dimorpholinopyridazine 0.01
Methyl bis (trimethylsiloxy) trimethoxycinnamate
Silylisopentyl 0.1
Yellow iron oxide Appropriate amount Cobalt titanate Appropriate amount Dimethyl distearyl ammonium hectorite 1.5
Polyvinyl alcohol 0.1
Hydroxyethyl cellulose 0.1
Purified water Residue Trimethylsiloxysilicic acid 2
Perfume

Example 2
Latex
Compounding amount (parts by weight)
────────────────────────────────────
Methyl polysiloxane 2
Behenyl alcohol 1
Xylitol 1
Batyl alcohol 0.5
Glycerin 5
1,3-butylene glycol 7
Erythritol 2
Hardened oil 3
Squalane 6
Tetra-2-ethylhexanoic acid pentaerythlit 2
Polyoxyethylene glyceryl isostearate 1
Polyoxyethylene glycerol monostearate 1
Ansoukou extract 0.001
Potassium hydroxide appropriate amount Sodium hexametaphosphate 0.05
Phenoxyethanol appropriate amount Carboxyvinyl polymer 0.11
Purified water residue

Example 3
Lotion
Compounding amount (parts by weight)
────────────────────────────────────
Glycerin 2
1,3-butylene glycol 4
Erythritol 1
Polyoxyethylene methyl glucoside 1
Polyoxyethylene hydrogenated castor oil 0.5
Citric acid 0.02
Sodium citrate 0.08
Phenoxyethanol 0.25
N-coconut oil fatty acid acyl L-arginine ethyl DL-
Pyrrolidone carboxylic acid 0.1
Key Kazura 0.0001
Purified water residue

Example 4
Whitening lotion
Compounding amount (parts by weight)
────────────────────────────────────
Ethyl alcohol 10
Dipropylene glycol 1
Polyethylene glycol 1000 1
Polyoxyethylene methyl glucoside 1
Jojoba oil 0.01
Glyceryl tri-2-ethylhexanoate 0.1
Polyoxyethylene hydrogenated castor oil 0.2
Polyglyceryl diisostearate 0.15
N-stearoyl-sodium L-glutamate 0.1
Citric acid 0.04
Sodium citrate 0.18
Potassium hydroxide 0.4
Dipotassium glycyrrhizinate 0.1
Arginine hydrochloride 0.1
L-ascorbic acid 2-glucoside 2
Golden extract 0.1
Yukinoshita Extract 0.1
Paraben 0.12
Odoriko Extract 0.1
Dibutylhydroxytoluene 0.01
Edetate trisodium 0.05
2-Ethylhexyl paramethoxycinnamate 0.01
Soap extract 0.005
Purified water Residual Mineral water 3
Perfume

Example 5
Treatment mask
Compounding amount (parts by weight)
────────────────────────────────────
Ethanol 10
1,3-butylene glycol 6
Polyethylene glycol 4000 2
Olive oil 1
Macadamia nut oil 1
Phytosteryl hydroxystearate 0.05
Lactic acid 0.05
Sodium lactate solution (50%) 0.2
L-ascorbic acid sulfate disodium salt 0.1
DL-α-tocopherol 2-L-ascorbic acid phosphate diester potassium 0.1
Vitamin E acetate 0.1
Fish collagen 0.1
Chondroitin thorium sulfate 0.1
Paraoxybenzoic acid ester 0.1
Sodium carboxymethylcellulose 0.2
Polyvinyl alcohol 12.5
Tranexamic acid 1
Sandalwood extract powder 0.001
Purified water Residue Fragrance Appropriate amount

Example 6
Whitening essence
Compounding amount (parts by weight)
────────────────────────────────────
Vaseline 2
Methyl polysiloxane 2
Ethyl alcohol 5
Behenyl alcohol 0.5
Batyl alcohol 0.2
Glycerin 7
1,3-butylene glycol 5
Polyethylene glycol 20000 0.5
Jojoba oil 3
Squalane 2
Cholesteryl hydroxystearate 0.5
Tetra-2-ethylhexanoic acid pentaerythrite 1
Polyoxyethylene hydrogenated castor oil 1
Potassium hydroxide 0.1
Sodium pyrosulfite 0.01
Sodium hexametaphosphate 0.05
Stearyl glycyrrhetinate 0.1
Pantothenyl ethyl ether 0.1
Arbutin 7
Tocopherol acetate 0.1
Sodium hyaluronate 0.05
Eucalyptus extract 0.001
P-Hydroxybenzoate appropriate amount edetate trisodium 0.05
4-t-butyl-4'-methoxydibenzoylmethane 0.1
Diparamethoxycinnamate mono-2-ethylhexanoate glyceryl 0.1
Yellow iron oxide Appropriate amount Xanthan gum 0.1
Carboxyvinyl polymer 0.2
Purified water residue

Example 7
Itching bath agent
Compounding amount (parts by weight)
────────────────────────────────────
Liquid paraffin 35
Polyethylene glycol 20
Squalane 5
Cetyl 2-ethylhexanoate 5
Polyoxyethylene hydrogenated castor oil 5
Citric acid 0.1
Sodium citrate 0.2
Glycine 0.5
Sodium hyaluronate 0.3
Phenoxyethanol 0.5
Parietalia extract 1
Purified water Residue Fragrance

Inhibition of SCF by various herbal extracts on cells stimulated by drying. Inhibition of SCF by various herbal extracts on cells stimulated by drying. Enhancement of SCF expression in cells by UV stimulation. Enhancement of SCF expression in cells by drug stimulation. Inhibition of SCF by various herbal extracts on cells stimulated with ultraviolet light.

Claims (2)

  As an effective ingredient to improve sensitive skin, rose extract rose water, tea extract, hop extract, hawthorn extract, azuki bean powder, birch extract, kehi extract, clove extract, chlorella extract, black tea extract, sojutsu extract powder, peanut extract powder, oolong tea extract powder, onionis extract , Asenyaku extract, Grape leaf extract, Bow extract, Mulberry extract, Parietalia extract, Ansock extract, Stevia extract, Cypress, Ginger root extract, Soybean extract, Kakizura, Sabonso extract, Altea extract and Artemisia extract A topical skin preparation for sensitive skin, comprising a component that suppresses the production and / or release of one or more SCFs.   Sensitive skin comprising applying an active ingredient that exhibits an effect of improving pruritus, rough skin or sensitive skin by inhibiting the production and / or release of SCF as defined in claim 1 to human or mammalian epidermis How to improve.

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