KR19990086516A - Anti-aging cosmetic composition containing ginseng extract - Google Patents

Anti-aging cosmetic composition containing ginseng extract Download PDF

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KR19990086516A
KR19990086516A KR1019980019526A KR19980019526A KR19990086516A KR 19990086516 A KR19990086516 A KR 19990086516A KR 1019980019526 A KR1019980019526 A KR 1019980019526A KR 19980019526 A KR19980019526 A KR 19980019526A KR 19990086516 A KR19990086516 A KR 19990086516A
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ginseng extract
skin
cosmetic composition
ginseng
extract
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KR1019980019526A
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KR100361433B1 (en
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오지연
박이근
송동혁
염명훈
조윤기
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서경배
주식회사 태평양
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations

Abstract

본 발명은 피부노화 방지용 화장료 조성물에 관한 것으로, 피부세포 증식 및 콜라겐 생합성 촉진 효과를 갖는 인삼(Panax ginsengC.A Meyer)의 추출물을 함유시킴으로써 피부 노화방지에 우수한 화장료 조성물을 제공할 수 있다.The present invention relates to a cosmetic composition for preventing skin aging, and it is possible to provide a cosmetic composition excellent in skin aging prevention by containing an extract of ginseng ( Panax ginseng C.A Meyer) having skin cell proliferation and promoting collagen biosynthesis.

Description

인삼추출물을 함유하는 피부 노화방지용 화장료 조성물Anti-aging cosmetic composition containing ginseng extract

본 발명은 인삼의 추출물을 함유하는 피부 노화방지용 화장료 조성물에 관한 것으로, 보다 상세하게는 피부 세포의 증식 및 콜라겐 생합성 촉진 효과를 갖는 인삼(Panax ginsengC.A Meyer)의 추출물을 함유함으로써 피부 노화방지 효과를 갖는 화장료 조성물에 관한 것이다.The present invention relates to a cosmetic composition for preventing skin aging which contains an extract of ginseng. More particularly, the present invention relates to a cosmetic composition for inhibiting skin aging by containing an extract of Panax ginseng CA Meyer having the effect of promoting skin cell proliferation and collagen biosynthesis To a cosmetic composition containing the same.

피부는 인체의 일차 방어막으로서 체내의 제기관을 온도 및 습도 변화와 자외선, 공해물질 등 외부환경의 자극으로부터 보호해 주며, 체온조절 등의 생체 항상성 유지에 중요한 역할을 하고 있다. 그러나, 외부로부터 받는 과도한 물리적, 화학적 자극 및 스트레스, 영양결핍 등은 피부의 정상기능을 저하시키고 탄력손실, 각질화, 주름생성 등의 피부 노화현상을 촉진시키게 되는데, 이러한 현상을 방지하고 보다 건강하고 탄력있는 피부를 유지하기 위하여 종래 각종 동물, 식물, 미생물 등으로부터 얻은 생리활성물질들이 강화된 화장품을 사용함으로써 피부의 고유기능을 유지시키고 피부세포를 활성화시켜 피부노화를 효과적으로 억제하기 위한 노력이 있어 왔었다. 그러나, 이러한 기존의 화장품 원료들은 대부분 그 효능이 미진하거나 피부 부작용을 유발하는 등 여러 가지 문제점을 가지고 있다.Skin is the primary barrier of the human body and protects the internal organs of the body from changes in temperature and humidity, from external stimuli such as ultraviolet rays and pollutants, and plays an important role in the maintenance of body homeostasis such as body temperature control. However, the excessive physical and chemical stimuli and stress from the outside, stress and nutritional deficiency deteriorate the normal function of the skin and promote the skin aging phenomenon such as loss of elasticity, keratinization and wrinkle formation. There has been an effort to maintain skin's natural functions and activate skin cells to effectively inhibit skin aging by using cosmetics reinforced with physiologically active substances obtained from various animals, plants, microorganisms, However, most of these conventional cosmetic raw materials have various problems such that they have little effect or cause skin side effects.

이에, 본 출원인들은 피부에 안전하면서도 피부노화의 주요 원인인 피부의 각질형성세포, 섬유아세포의 재생감소 그리고 이로부터 생성되는 콜라겐의 생합성량 감소를 효과적으로 억제할 수 있는 피부 노화방지용 화장료를 제공하기 위하여 예의 연구하던 중, 인삼(Panax ginsengC.A. Meyer)의 추출물이 피부노화를 방지할 수 있는 효과가 있음을 발견하고 본 발명을 완성하게 되었다.Accordingly, the present applicants have proposed a cosmetic for preventing skin aging which can effectively inhibit the reduction of regeneration of keratinocytes, fibroblasts and the biosynthesis amount of collagen produced from the skin, which is a major cause of skin aging. In studying the example, it was found that the extract of Panax ginseng C.A. Meyer has an effect of preventing skin aging and completed the present invention.

따라서, 본 발명의 목적은 피부 노화방지용 화장료 조성물을 제공하는 것이다.Accordingly, an object of the present invention is to provide a cosmetic composition for preventing skin aging.

상기한 목적을 달성하기 위하여, 본 발명의 피부 노화방지용 화장료 조성물은 인삼 추출물을 조성물 총 중량에 대하여 건조 중량으로 10-8∼101중량%의 양으로 함유함을 특징으로 한다.In order to achieve the above object, the cosmetic composition for preventing skin aging according to the present invention is characterized in that the ginseng extract is contained in an amount of 10 -8 to 10 1 % by dry weight based on the total weight of the composition.

상기한 본 발명의 목적, 그외의 목적, 특징 및 장점은 하기 발명의 상세한 설명으로부터 당업자에게 명백하게 드러날 것이다.The above and other objects, features and advantages of the present invention will become apparent to those skilled in the art from the following detailed description.

이하, 본 발명을 보다 상세하게 설명한다.Hereinafter, the present invention will be described in more detail.

인삼(Panax ginsengC.A. meyer)은 오가피과 인삼속에 속하는 식물로 한국, 중국, 일본 등지에서 2,000여년 전부터 사용되어 온 생약으로, 경험적으로 질병을 예방하고 수명을 연장시킬 목적으로 사용되어 왔으며, 피부의 기능을 항진시켜 피부를 건강하게 유지시키는 효과가 있다고 알려져 있다. 또한, 인삼의 추출물은 항보체 활성, 항암작용, 혈당강하작용, 항궤양 작용 그리고 면역증강 작용이 있는 것으로 최근에 많은 연구를 통해 보고되고 있다. 이러한 연구는 주로 인삼을 에탄올 수용액으로 추출한 후, 이를 용매분획을 실시하여 실리카겔 칼럼크로마토그래피법 또는 C-18 칼럼크로마토그래피법을 이용하여 단일성분을 분리하는 방법에 의한 것으로, 이러한 방법에 의해서는 사포닌, 진세노사이드 등의 인삼 중의 에탄올 가용부분만이 활용되었으며, 에탄올 불용부분은 폐기되었다.Ginseng ( Panax ginseng CA meyer) is a herb that has been used in Korea, China, and Japan for over 2,000 years. It has been used for the purpose of preventing diseases and prolonging the life span. It is said to be effective in keeping the skin healthy by aggression. In addition, ginseng extract has been reported to have anticomplement activity, anticancer activity, hypoglycemic action, anti-ulcer activity and immunity enhancement in recent studies. These studies are mainly based on a method in which ginseng is extracted with an aqueous solution of ethanol and subjected to solvent fractionation to separate a single component by a silica gel column chromatography method or a C-18 column chromatography method. According to this method, saponin , And ginsenosides, and the ethanol insoluble portion was discarded.

그러나, 본원 발명에서는 기존에 폐기처리되던 에탄올 불용부분을 활용하여 인삼의 추출물이 피부의 노화방지에 효과적이라는 것을 발견한 것으로, 본원 발명에서 인삼 추출물은 인삼(Panax ginsengC.A. Meyer) 약 10kg에 95% 에탄올 약 100ℓ를 가하여 3시간동안 가열, 환류, 추출하여 에탄올 가용성분을 제거한 다음, 불용성분을 포함한 잔사를 다시 물 약 100ℓ로 3시간동안 가열, 환류, 추출하여 규조토로 여과한 후에 에탄올 약 250ℓ를 가하여 침전시킨 다음 이 침전물을 건조시킴으로써 얻어진다.However, in the present invention, it has been found that the extract of ginseng is effective for preventing aging of skin by utilizing the ethanol-insoluble portion which has been discarded in the present invention. In the present invention, the ginseng extract is a mixture of about 10 kg of ginseng ( Panax ginseng CA Meyer) About 100 L of ethanol was added, and the mixture was heated, refluxed and extracted for 3 hours to remove ethanol-soluble components. The residue containing insoluble components was further heated with reflux for about 3 hours, filtered, and filtered through diatomaceous earth. Followed by precipitation, followed by drying of the precipitate.

본 발명의 피부 노화방지 화장료 조성물은 상기 인삼 추출물을 조성물 총 중량에 대하여 10-8∼101중량%, 바람직하게는 10-5∼5.0중량%의 양으로 함유된다.The anti-aging cosmetic composition of the present invention contains the ginseng extract in an amount of 10 -8 to 10 1 % by weight, preferably 10 -5 to 5.0% by weight based on the total weight of the composition.

본 발명의 피부 노화방지용 화장료 조성물은 그 제형에 있어서 특별히 한정되는 바가 없으며, 예를 들면, 유연화장수, 수렴화장수, 영양화장수, 영양크림, 마사지 크림, 에센스, 아이크림, 아이에센스, 클렌징크림, 클렌징폼, 클렌징워터, 팩, 파우더, 보디로션, 보디크림, 보디오일, 보디에센스 등의 화장료로 제형화될 수 있다.The cosmetic composition for preventing skin aging according to the present invention is not particularly limited in its formulation, and examples thereof include softening lotion, convergent lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, eye essence, cleansing cream, Foam, cleansing water, pack, powder, body lotion, body cream, body oil, body essence and the like.

또한, 각 제형의 피부 노화방지용 화장료 조성물에 있어서, 상기한 인삼 추출물 이외의 성분들은 기타 화장료의 제형 또는 사용목적 등에 따라 당업자가 어려움없이 적의 선정하여 배합할 수 있다.In addition, in the cosmetic composition for preventing skin aging of each formulation, components other than the ginseng extract described above can be mixed and selected without difficulty by those skilled in the art according to the formulation or purpose of use of other cosmetics.

이하, 본 발명의 시험예를 들어 본 발명의 구성 및 그 효과에 대하여 보다 구체적으로 설명한다.Hereinafter, the constitution of the present invention and the effect thereof will be described in more detail with reference to the test examples of the present invention.

〈시험예 1〉섬유아세포(Fibroblast)의 증식효능 측정 ≪ Test Example 1 > Measurement of proliferation efficacy of fibroblast

3.5%의 우태아 혈청이 함유된 DMEM(Dulbecco's Modified Eagle's Media)배지에서 배양한 인체 섬유아세포를 96공 평판배양기(96-well microtiter plate)에 5,000세포/well가 되도록 분주하고, 인삼 추출물을 배양배지로 1/10씩 순차적으로 희석하여 첨가한 후, 37℃ 온도에서 4일간 배양하였다. 배양 후 0.2% MTT(3-[4, 5-dimethylthiazol-2-yl] -2, 5-diphenyltertazolium bromide) 용액을 각 well당 50㎕씩 첨가하고, 다시 37℃ 온도에서 4시간동안 배양한 후 생성된 포르마잔(formazan)을 DMSO(Dimethyl sulfoxide)로 용해시켰다. 용해된 포르마잔의 흡광도를 평판배양측정기(microplate reader)를 이용하여 570nm에서 측정하였다. 이를 인삼 추출물을 처리하지 않은 대조군에 대하여 상기와 동일한 방법으로 실시하여 흡광도를 측정하였다. 인삼 추출물을 함유한 실험군과 대조군의 흡광도를 비교한 후, 그 결과를 표 1에 나타내었다.Human fibroblasts cultured in DMEM (Dulbecco's Modified Eagle's Media) medium containing 3.5% fetal bovine serum were dispensed in a 96-well microtiter plate to a concentration of 5,000 cells / well, and the ginseng extract was added to a culture medium , And the cells were cultured at 37 DEG C for 4 days. After incubation, 50 μl of 0.2% MTT (3- [4,5-dimethylthiazol-2-yl] -2,5-diphenyltertazolium bromide) solution was added to each well and incubated at 37 ° C for 4 hours The formazan was dissolved in dimethyl sulfoxide (DMSO). Absorbance of dissolved formazan was measured at 570 nm using a microplate reader. The absorbance was measured for the control group not treated with the ginseng extract in the same manner as above. The absorbance of the ginseng extract and the control group were compared, and the results are shown in Table 1.

시료농도(%)Sample concentration (%) 세포증식능(%)Cell proliferation (%) 1 × 10-8 1 × 10 -8 44 1 × 10-7 1 × 10 -7 77 1 × 10-6 1 x 10 -6 1010 1 × 10-5 1 x 10 -5 1515 1 × 10-4 1 x 10 -4 2323 1 × 10-3 1 x 10 -3 5050 1 × 10-2 1 x 10 -2 5757 1 × 10-1 1 x 10 -1 6565

상기 표 1에서 알 수 있는 바와 같이, 인삼 추출물을 처리하지 않은 섬유아세포에 비하여, 인삼 추출물을 처리한 섬유아세포는 최고 약 65% 정도 향상된 섬유아세포 증식 효능을 나타내었다.As shown in Table 1, the fibroblasts treated with ginseng extract showed up to about 65% higher fibroblast proliferation than the fibroblasts not treated with ginseng extract.

〈시험예 2〉각질형성세포(Keratinocyte)의 증식효능 측정 Test Example 2 Measurement of proliferative effect of keratinocyte

각질형성세포를 사용하여 시험예 1에서와 동일한 방법으로 각질형성세포의 증식효능을 측정하여, 그 결과를 표 2에 나타내었다.Using the keratinocytes, the proliferative activity of keratinocytes was measured in the same manner as in Test Example 1, and the results are shown in Table 2.

시료농도(%)Sample concentration (%) 세포증식능(%)Cell proliferation (%) 1 × 10-8 1 × 10 -8 44 1 × 10-7 1 × 10 -7 88 1 × 10-6 1 x 10 -6 99 1 × 10-5 1 x 10 -5 1313 1 × 10-4 1 x 10 -4 1515 1 × 10-3 1 x 10 -3 2424 1 × 10-2 1 x 10 -2 3030 1 × 10-1 1 x 10 -1 3333

상기 표 2에서 알 수 있는 바와 같이, 인삼 추출물을 처리하지 않은 각질형성세포에 비하여, 인삼 추출물을 처리한 각질형성세포는 최고 약 33% 정도 향상된 각질세포 증식 효능을 나타내었다.As shown in Table 2, keratinocytes treated with ginseng extract showed up to 33% higher keratinocyte proliferative activity than keratinocytes not treated with ginseng extract.

〈시험예 3〉섬유아세포의 콜라겐 합성정도의 측정 ≪ Test Example 3 & gt ; Measurement of degree of collagen synthesis of fibroblasts

인체 섬유아세포를 24공 평판배양기에 배양한 후, 인삼 추출물을 배양배지로 1/10씩 순차적으로 희석하여 첨가하였다. 배양 3일째 10%의 우태아 혈청이 함유된 DMEM배지를 각 well당 0.5㎖씩 첨가한 후 L[2, 3, 4, 5-3H]-프롤린 10μCi를 첨가하였다. 24시간 후 각 well에 들어있는 배지와 세포들을 긁어모아 5% 트리클로로아세틱엑씨드(TCA; Trichloroacetic acid) 용액에 넣어 수세한 후 2개의 시험관에 분주하고, 1개의 시험관에는 타입 I 콜라게나제(type I collagenase) 1unit/㎕를 넣고 37℃ 온도에서 90분간 배양하였으며, 다른 시험관은 4℃에서 보관하였다. 그후 모든 시험관에 50% TCA를 0.05㎖씩 첨가하고 4℃에서 20분간 방치한 후 각각 12,000rpm에서 10분간 원심분리하여 각각의 상등액과 침전물을 액체 신틸레이션 계수기(LSC; Liquid Scintillation Counter)로 디피엠(dpm; decay per minute) 값을 얻어 하기 수학식 1에 의거하여 인삼 추출물을 처리하지 않은 대조군과 실험군에 대한 콜라겐 생합성 값(RCB; Relative Collagen Biosynthesis)을 구하고, 그 결과를 하기 표 3에 나타내었다.Human fibroblasts were cultured in a 24-well plate incubator, and ginseng extract was diluted with 1/10 of the culture medium. After the addition of culture DMEM medium supplemented with fetal calf serum containing 10% by the third day each well per 0.5㎖ L [2, 3, 4 , 5- 3 H] - was added proline 10μCi. After 24 hours, the media and cells in each well were scraped off and washed in 5% trichloroacetic acid (TCA) solution, dispensed into 2 test tubes, and one test tube was treated with type I collagenase type I collagenase) was incubated at 37 ° C for 90 minutes and the other tubes were stored at 4 ° C. After that, 0.05 ml of 50% TCA was added to all the tubes, and the mixture was allowed to stand at 4 ° C for 20 minutes. Then, the tubes were centrifuged at 12,000 rpm for 10 minutes, and the supernatant and the precipitate were separated by a liquid scintillation counter (LSC) dpm ; decay per minute) value. Based on Equation (1), the collagen biosynthesis value ( RCB ) for the control group and the experimental group not treated with the ginseng extract was determined, and the results are shown in Table 3 below.

시료농도(%)Sample concentration (%) 콜라겐 합성능(%)Collagen Total Performance (%) 1 × 10-8 1 × 10 -8 33 1 × 10-7 1 × 10 -7 33 1 × 10-6 1 x 10 -6 88 1 × 10-5 1 x 10 -5 1111 1 × 10-4 1 x 10 -4 1717 1 × 10-3 1 x 10 -3 2525 1 × 10-2 1 x 10 -2 2626 1 × 10-1 1 x 10 -1 3131

상기 표 3에서 알 수 있는 바와 같이, 인삼 추출물을 처리하지 않은 섬유아세포에 비하여, 인삼 추출물을 처리한 섬유아세포는 최고 약 31% 정도 향상된 섬유아세포의 콜라겐 생합성 촉진효능을 나타내었다.As can be seen from Table 3, the fibroblasts treated with ginseng extract showed up to about 31% higher promoting activity of collagen biosynthesis than fibroblasts treated with ginseng extract.

〈시험예 4〉피부 자극시험 ≪ Test Example 4 > Skin irritation test

본원 발명의 인삼 추출물의 피부자극정도를 실험하기 위하여, 20∼30세의 건강한 여성 30명을 대상으로 CTFA 가이드라인(The Cosmetic, Toiletry and Fragrance Association. Inc. Washington, D.C., 20036, 1991)에 따라 실시하였다. 핀 챔버(Finn Chamber)에 준비된 시험물질(10% in Patch base) 20㎕를 적하시킨 후 70% 에탄올로 세척한 후 건조시킨 시험부위인 등 부위에 얹어 미소공 테이프(micropore tape)로 고정시켰다. 첩포는 24시간동안 도포하며, 첩포를 제거한 후 마킹팬(marking pen)으로 시험 부위를 표시하며 30분, 24시간 후에 각 시험 부위의 피부반응을 관찰하여 국제 접촉 피부염 연구회(International Contact Dermatitis Research Group: ICDRG)의 규정에 따라 판정하였다. 하기 수학식 2를 이용하여 평균반응도를 계산한 후 그 결과를 하기 표 4에 나타내었다. 또한, 평균반응도를 기초로 하여 피부자극 정도를 다음의 4등급으로 분류하였다.To examine the degree of skin irritation of the ginseng extract according to the present invention, 30 healthy women aged 20 to 30 years were divided into three groups according to the CTFA guidelines (The Cosmetic, Toiletry and Fragrance Association Inc. Washington, DC, 20036, 1991) Respectively. 20 μl of the test substance (10% in Patch base) prepared in the Finn Chamber was dripped, washed with 70% ethanol, placed on the back of the test site, and fixed with a micropore tape. The patches were applied for 24 hours. After removing the patches, the test area was marked with a marking pen. After 30 minutes and 24 hours, the skin reaction of each test site was observed and the results were analyzed by the International Contact Dermatitis Research Group ICDRG). The average reactivity was calculated using the following equation (2), and the results are shown in Table 4 below. The degree of skin irritation was classified into the following four grades based on the average degree of reaction.

·피부자극 판정 기준 :· Skin irritation criteria:

Ⅰ등급 …… MS＜1(무자극) Ⅱ등급 …… 1≤MS＜3(경자극)Class I ... ... MS <1 (no stimulation) Ⅱ grade ... ... 1 &lt; / = MS &lt; 3 (light stimulus)

Ⅲ등급 …… 3≤MS＜5(중자극) Ⅳ등급 …… MS≥5(강자극)Ⅲ grade ... ... 3 ≤ MS <5 (moderate irritation) Ⅳ grade ... ... MS ≥ 5 (strong stimulus)

시험물질Test substance 24시간24 hours 48시간48 hours 평균반응도(n=30)Mean response (n = 30) 판정등급Judgment grade ±± ++ ++++ ++++++ ±± ++ ++++ ++++++ 실시예 1(농도: 10% 염수)Example 1 (concentration: 10% brine) 1One ­ ­ ­ ­ ­ ­ ­ 0.420.42 ⅠⅠ 실시예 2(농도: 1% 염수)Example 2 (concentration: 1% brine) 33 ­ ­ ­ ­ ­ ­ ­ 1.251.25 ⅡⅡ

상기 표 4에서 알 수 있는 바와 같이, 본 발명의 인삼추출물은 피부자극이 거의 없슴을 확인할 수 있었다.As can be seen from Table 4, it can be confirmed that the ginseng extract of the present invention has little skin irritation.

이상의 시험예에서 알 수 있는 바와 같이, 인삼추출물은 피부자극이 없으면서 피부세포 증식 및 콜라겐의 생합성을 촉진하는 물질임을 알 수 있다.As can be seen from the above test results, it can be seen that the ginseng extract promotes skin cell proliferation and collagen biosynthesis without skin irritation.

〈시험예 4〉인체피부를 대상으로 한 피부주름 개선효과 &Lt; Test Example 4 &gt; Effect of improving skin wrinkles on human skin

35∼45세의 안면주름이 있는 시험대상자 30명에 대하여, 하기 표 4와 같은 조성을 갖는 영양크림에 대하여 인삼 추출물을 함유한 실시예 1과 인삼 추출물을 함유하지 않은 비교예 1의 피부주름 개선효과를 비교평가하게 하였다.For the 30 subjects who had facial wrinkles of 35 to 45 years old, the nutritional cream having the composition as shown in Table 4 below was compared with Example 1 containing ginseng extract and Comparative Example 1 containing no ginseng extract. Were compared.

성 분ingredient 단위 : 중량%Unit: wt% 제형예 1Formulation Example 1 비교예 1Comparative Example 1 인삼 추출물Ginseng extract 0.10.1 -- 밀납Wax 10.010.0 10.010.0 폴리솔베이트 60Polysorbate 60 1.51.5 1.51.5 피이지 60 경화피마자유Piggy 60 hardened castor oil 2.02.0 2.02.0 솔비탄세스퀴올레이트Sorbitan sesquioleate 0.50.5 0.50.5 유동파라핀Liquid paraffin 10.010.0 10.010.0 스쿠알란Squalane 5.05.0 5.05.0 카프릴릭/카프릭트리글리세라이드Caprylic / capric triglyceride 5.05.0 5.05.0 글리세린glycerin 5.05.0 5.05.0 부틸렌글리콜Butylene glycol 3.03.0 3.03.0 프로필렌글리콜Propylene glycol 3.03.0 3.03.0 트리에탄올아민Triethanolamine 0.20.2 0.20.2 방부제, 색소, 향료Preservative, coloring, fragrance 적량Suitable amount 적량Suitable amount 정제수Purified water to 100to 100 to 100to 100

피검자의 안면 좌부에는 제형예 1을, 우부에는 비교예 1을 3개월간 사용하도록 하였다. 크림사용 이전의 안면 양쪽부의 피부상태를 측정해 놓은 후 크림사용 3개월 후 동일부위를 재측정하여 피부주름의 변화를 측정하였다. 피부측정은 24℃ 온도, 상대습도 40%의 항온항습실에서 하였으며, 눈꼬리부위의 주름을 레플리카(replica)로 떠서 비시오메타 시스템(Visiometer system; C+K사)으로 피부주름을 측정하였다. 피부주름의 변화량은 하기 수학식 3에 따라 계산하였다.Formulation Example 1 was used for the face side of the subject and Comparative Example 1 was used for the right side for 3 months. After the skin condition of both sides of the face before the use of the cream was measured, the same part was remeasured 3 months after using the cream to measure the change of the skin wrinkles. Skin wrinkles were measured with a Visiometer system (C + K) by removing the wrinkles of the eye tails with a replica, at a temperature of 24 ° C and a relative humidity of 40%. The change amount of the skin wrinkles was calculated according to the following equation (3).

(상기 식에서,(Wherein,

Tdi; D90에서의 측정부위 값이며,Tdo; D0에서의 측정부위 값이다) Tdi ; A measured position value at D 90, Tdo; D 0 )

상기 식에 따라 계산한 결과, 비교예 1을 사용한 부위의 피부주름은 8±5%(평균±표준편차)의 감소치를 나타낸 반면, 실시예 1을 사용한 부위의 피부주름은32±13%의 감소치를 보여 우수한 피부주름 개선효과를 나타내었다.As a result of calculation according to the above formula, skin wrinkles at the site using Comparative Example 1 showed a decrease of 8 ± 5% (mean ± standard deviation), whereas skin wrinkles at the site using Example 1 decreased by 32 ± 13% And showed excellent skin wrinkle improving effect.

이하, 상기한 시험예들의 결과를 근거로 하여, 인삼 추출물을 함유함으로써 피부 노화방지 효과를 제공할 수 있는 여러 제형의 화장료를 조성하여 제시한다. 그러나 본 발명의 조성물이 하기의 제형예에 한정되는 것은 아니다.Based on the results of the above-described test examples, various cosmetic formulations capable of providing an anti-aging effect by containing ginseng extract are prepared and presented. However, the composition of the present invention is not limited to the following formulation examples.

(제형예 2) 유연화장수(스킨로션)(Formulation Example 2) Flexible longevity (skin lotion)

배합성분Compounding ingredient 중량%weight% 인삼 추출물Ginseng extract 0.10.1 글리세린glycerin 3.03.0 부틸렌글리콜Butylene glycol 2.02.0 프로필렌글리콜Propylene glycol 2.02.0 카르복시비닐폴리머Carboxyvinyl polymer 0.10.1 피이지-12 노닐페닐에테르Phage-12 nonylphenyl ether 0.20.2 폴리솔베이트 80Polysorbate 80 0.40.4 에탄올ethanol 10.010.0 트리에탄올아민Triethanolamine 0.10.1 방부제, 색소, 향료Preservative, coloring, fragrance 적량Suitable amount 정제수Purified water to 100to 100

(제형예 3) 영양화장수(밀크로션)(Formulation Example 3) Nutritional lotion (milk lotion)

배합성분Compounding ingredient 중량%weight% 인삼 추출물Ginseng extract 0.50.5 스쿠알란Squalane 5.05.0 밀납Wax 4.04.0 폴리솔베이트 60Polysorbate 60 1.51.5 솔비탄세스퀴올레이트Sorbitan sesquioleate 1.51.5 유동파라핀Liquid paraffin 0.50.5 카프릴릭/카프릭트리글리세라이드Caprylic / capric triglyceride 5.05.0 글리세린glycerin 3.03.0 부틸렌글리콜Butylene glycol 3.03.0 프로필렌글리콜Propylene glycol 3.03.0 카르복시비닐폴리머Carboxyvinyl polymer 0.10.1 트리에탄올아민Triethanolamine 0.20.2 방부제, 색소, 향료Preservative, coloring, fragrance 적량Suitable amount 정제수Purified water to 100to 100

(제형예 4) 영양크림(Formulation Example 4) Nourishing cream

배합성분Compounding ingredient 중량%weight% 인삼 추출물Ginseng extract 2.02.0 밀납Wax 10.010.0 폴리솔베이트 60Polysorbate 60 1.51.5 피이지 60 경화피마자유Piggy 60 hardened castor oil 2.02.0 솔비탄세스퀴올레이트Sorbitan sesquioleate 0.50.5 유동파라핀Liquid paraffin 10.010.0 스쿠알란Squalane 5.05.0 카프릴릭/카프릭트리글리세라이드Caprylic / capric triglyceride 5.05.0 글리세린glycerin 5.05.0 부틸렌글리콜Butylene glycol 3.03.0 프로필렌글리콜Propylene glycol 3.03.0 트리에탄올아민Triethanolamine 0.20.2 방부제, 색소, 향료Preservative, coloring, fragrance 적량Suitable amount 정제수Purified water to 100to 100

(제형예 5) 마사지크림(Formulation Example 5) Massage cream

배합성분Compounding ingredient 중량%weight% 인삼 추출물Ginseng extract 5.05.0 밀납Wax 10.010.0 폴리솔베이트 60Polysorbate 60 1.51.5 피이지 60 경화피마자유Piggy 60 hardened castor oil 2.02.0 솔비탄세스퀴올레이트Sorbitan sesquioleate 0.80.8 유동파라핀Liquid paraffin 40.040.0 스쿠알란Squalane 5.05.0 카프릴릭/카프릭트리글리세라이드Caprylic / capric triglyceride 4.04.0 글리세린glycerin 5.05.0 부틸렌글리콜Butylene glycol 3.03.0 프로필렌글리콜Propylene glycol 3.03.0 트리에탄올아민Triethanolamine 0.20.2 방부제, 색소, 향료Preservative, coloring, fragrance 적량Suitable amount 정제수Purified water to 100to 100

(제형예 6) 팩(Formulation Example 6) Pack

배합성분Compounding ingredient 중량%weight% 인삼 추출물Ginseng extract 5.05.0 폴리비닐알콜Polyvinyl alcohol 13.013.0 소듐카르복시메틸셀룰로오스Sodium carboxymethylcellulose 0.20.2 글리세린glycerin 5.05.0 알란토인Allantoin 0.10.1 에탄올ethanol 6.06.0 핑지-12 노닐페닐에테르Fingi-12 nonylphenyl ether 0.30.3 폴리솔베이트 60Polysorbate 60 0.30.3 방부제, 색소, 향료Preservative, coloring, fragrance 적량Suitable amount 정제수Purified water to 100to 100

(제형예 7) 젤(Formulation Example 7)

배합성분Compounding ingredient 중량%weight% 인삼 추출물Ginseng extract 1.01.0 에틸렌디아민초산나트륨Ethylenediamine sodium acetate 0.050.05 글리세린glycerin 5.05.0 카르복시비닐폴리머Carboxyvinyl polymer 0.30.3 에탄올ethanol 5.05.0 피이지-60 경화피마자유Piggy-60 hardened castor oil 0.50.5 트리에탄올아민Triethanolamine 0.30.3 방부제, 색소, 향료Preservative, coloring, fragrance 적량Suitable amount 정제수Purified water to 100to 100

이상에서 설명한 바와 같이, 피부세포 증식 및 콜라겐 생합성 촉진효과를 갖는 인삼 추출물을 화장료 조성물에 함유시킴으로써, 피부 노화방지에 효과적인 화장료 조성물을 제공할 수 있다.As described above, by containing the ginseng extract having skin cell proliferation and promoting collagen biosynthesis in the cosmetic composition, it is possible to provide a cosmetic composition effective for preventing skin aging.

Claims (1)

피부 노화방지용 화장료 조성물에 있어서, 인삼(Panax ginsengC.A. Meyer)의 에탄올 불용성 성분을 열수추출하여 규조토로 여과한 후, 에탄올을 가하여 침전시켜 얻은 인삼추출물을 조성물 총 중량에 대하여 10-8∼101중량%의 양으로 함유함을 특징으로 하는 피부 노화방지용 화장료 조성물.A cosmetic composition for preventing skin aging characterized in that an ethanol insoluble component of ginseng ( Panax ginseng C.A. Meyer) is extracted with hot water, filtered with diatomaceous earth and then precipitated with ethanol to give a ginseng extract at a concentration of 10 -8 to 10 1 %, Based on the total weight of the cosmetic composition.
KR1019980019526A 1998-05-28 1998-05-28 Anti-aging cosmetic compositions containing an Extract obtained from Panax ginseng KR100361433B1 (en)

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Cited By (10)

* Cited by examiner, † Cited by third party
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KR100365071B1 (en) * 2000-12-28 2002-12-18 주식회사 태평양 Skin Care Compositions for skin-aging prevention containing genseng aglycone
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KR100517899B1 (en) * 2002-01-30 2005-09-30 주식회사 일화 Bioconversion ginseng composite using microorganism and fabrication method thereof
KR100820072B1 (en) * 2002-12-28 2008-04-10 (주)아모레퍼시픽 Control agent of Bcl-2 expression being Ginsenoside F1 as active component
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KR100451284B1 (en) * 1998-12-26 2004-11-20 소망화장품주식회사 Cosmetic compositiion containing red ginseng as main component
KR100372360B1 (en) * 2000-09-06 2003-02-19 로제화장품 주식회사 Cosmetic compositions containing garlic and Acanthopanax extracts
KR100365071B1 (en) * 2000-12-28 2002-12-18 주식회사 태평양 Skin Care Compositions for skin-aging prevention containing genseng aglycone
KR100423521B1 (en) * 2001-08-29 2004-03-18 네비온 주식회사 Cosmetic compositions containing panax ginseng polysaccharides
KR100513902B1 (en) * 2001-12-24 2005-09-07 주식회사 코리아나화장품 Cosmetic Compositions Comprising Extract from Panax ginseng(C.A.MEY) for Preventing Skin Aging
KR100465976B1 (en) * 2002-01-05 2005-01-13 주식회사 태평양 Nanoemulsion having Ginsenoside F1 by nano-emulsion technology, and skin care and pharmaceutical compositions for external applications utilizing thereof
KR100517899B1 (en) * 2002-01-30 2005-09-30 주식회사 일화 Bioconversion ginseng composite using microorganism and fabrication method thereof
KR100920961B1 (en) * 2002-06-25 2009-10-09 가부시키가이샤 시세이도 Anti-aging agent
KR100820072B1 (en) * 2002-12-28 2008-04-10 (주)아모레퍼시픽 Control agent of Bcl-2 expression being Ginsenoside F1 as active component
KR101418826B1 (en) * 2011-03-18 2014-07-16 한규수 Cosmetic composition including herb medicine extract

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