KR100513902B1 - Cosmetic Compositions Comprising Extract from Panax ginseng(C.A.MEY) for Preventing Skin Aging - Google Patents

Abstract

The present invention relates to a cosmetic composition for preventing skin aging comprising camphor ginseng extract as an active ingredient, the cosmetic composition of the present invention promotes the proliferation of fibroblasts, promotes collagen synthesis, and moisturizing effect without irritation to the skin It is very useful for preventing skin aging.

Description

Cosmetic composition for preventing skin aging comprising camphor ginseng extract {Cosmetic Compositions Comprising Extract from Panax ginseng (C.A.MEY) for Preventing Skin Aging}

The present invention relates to a cosmetic composition, and more particularly to a cosmetic composition for preventing skin aging comprising Panax ginseng (C.A.MEY) extract as an active ingredient.

The primary function of the skin is to protect the human body from external stimuli such as temperature, humidity and ultraviolet light. However, when the skin is exposed to external stimuli such as strong ultraviolet rays, stress, and malnutrition, the skin may age, such as wrinkle formation, loss of elasticity, and keratinization. Collagen and elastic fibers (elastin) present in the dermal layer of the skin structure is the main protein that controls the elasticity of the skin. Biosynthesis of collagen fiber is internally and externally nourishment of the skin. Internally, aging (reduction of estrogen) causes cell activity to decrease and collagen fiber decreases. External factors include active oxygen (free radical type) generated by excessive irradiation of UV rays and stress, which react with the -SH group of the protein to inhibit the activity of the enzyme, or decompose collagen and elastin (Matrix Metalloproteinases: MMPS) increases the expression of collagenase, causing aging that reduces wrinkles and elasticity of the skin. In particular, over-irradiation of UV light may cause wrinkles, thickening of the skin, sagging of the skin, loss of elasticity, roughness of the skin, dryness of the skin, and skin stains that occur as skin tissue gradually changes with age. It is known to accelerate. Currently, cosmetics or skin protectants containing various skin protection ingredients such as retinol, retinoid, vitamin C, flavonoids and tocopherol have been developed to suppress or slow down the progression of skin. In the final formulation, the potency is low, the efficacy is mild, and the skin irritation has a serious problem.

On the other hand, Korean Patent Application No. 1997-60849 discloses a cosmetic composition for preventing skin aging containing serine of retinol, alpha hydroxy acid, vitamins, especially beta hydroxy acid, and Korea Patent Application No. 1998-18636 An anti-aging cosmetic composition comprising an amino polymer as an active ingredient is disclosed. In addition, Korean Patent Application No. 1994-7002880 discloses a synergistic cosmetic composition as an antioxidant containing ginkgo extract and at least one polyphenol derivative, and Korean Patent Application No. 1996-69236 discloses L-2- Disclosed is a skin anti-aging composition comprising an oxothiazolidine-4-carboxylic acid derivative.

Throughout this specification, numerous patent documents are referenced and their citations are indicated. The disclosures of the cited patents are incorporated herein by reference in their entirety, so that the level of the technical field to which the present invention belongs and the contents of the present invention are more clearly explained.

The present inventors earnestly researched to develop a cosmetic composition for preventing skin aging without side effects, and when the camphor extract was used, the present invention was completed by confirming that the above-described side effects, in particular, skin anti-aging effects are almost absent. .

Accordingly, it is an object of the present invention to provide a cosmetic composition for preventing skin aging.

The present invention provides a cosmetic composition for preventing skin aging comprising Panax ginseng (C.A.MEY) extract as an active ingredient.

In order to improve the problems of the conventional skin anti-aging cosmetic composition exhibiting adverse effects on the skin, the present inventors conducted a study on the native herbal medicines, and as a result, the camphor ginseng extract promotes collagen synthesis, an ingredient that imparts elasticity to the skin. In addition, it promotes the proliferation of fibroblasts, which are mainly composed of collagen synthesis, and also enhances the skin's moisturizing power, and has confirmed that there are few skin irritation side effects.

Camphor ginseng as an active ingredient in the composition of the present invention is a kind of wild ginseng, wild ginseng is classified into three types, cheonjong, jijong and camphor (race), and cheonjong is a natural wild ginseng, the algae after eating the seeds and excreted in the mountains It refers to wild ginseng seeded with thousands of seeds, and camphor ginseng is artificially grown wild in the wild by collecting seeds from deep ginseng. Among these wild ginseng, camphor ginseng, which is relatively easy to purchase, is known to have more saponin and a higher pharmacological effect than ginseng. Known pharmacological effects of camphor ginseng are the treatment of diabetes, liver disease, hypertension, gynecology and menopausal symptoms.

On the other hand, the term "camphor ginseng extract" in the present specification means that obtained by extracting from various organs (such as leaves, flowers, stems and roots) of camphor, preferably means an extract obtained from the root of camphor.

Camphor ginseng extract in the present invention can be obtained through various methods known in the art, preferably (a) water, (b) anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (e.g. methanol, ethanol, propanol , Butanol, and the like), (c) acetone, (d) ethyl acetate, (e) chloroform or (f) 1,3-butylene glycol were obtained from camphor ginseng. A suitable amount of the extraction solvent is 1-20 times the dry weight of camphor ginseng.

On the other hand, it will be apparent to those skilled in the art that the extract of the present invention can be obtained not only the above-described extraction solvent, but also the camphor ginseng extract having substantially the same effect using other extraction solvents.

In addition, the extract of the present invention includes not only the extract by the above-described extraction solvent, but also the extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using ultrafiltration membranes having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also included in the camphor ginseng extract of the present invention.

Camphor ginseng extract of the present invention may be prepared in a powder state by an additional process such as distillation under reduced pressure, freeze drying or spray drying.

In a preferred embodiment of the present invention, the content of camphor ginseng extract is 0.0001 to 10.0% by weight, more preferably 0.0005 to 10.0% by weight, most preferably 0.005 to 5.0% by weight based on the total cosmetic composition. When the content of camphor ginseng extract is less than 0.0001% by weight, no obvious anti-aging effect, especially wrinkle improvement, can be expected, and when the content of camphor ginseng extract is more than 10.0% by weight, there is no apparent increase in the content. .

Ingredients included in the cosmetic composition of the present invention include ingredients commonly used in cosmetic compositions in addition to camphor ginseng extract as an active ingredient, such as conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings, and Carrier.

The composition for alleviating the skin irritation of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactants -Can be formulated with, but not limited to, cleansing, oils, powder foundations, emulsion foundations, wax foundations, sprays, and the like. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The cosmetic composition of the present invention has the use of skin anti-aging. As used herein, the term "anti-aging of skin" has the meaning encompassing comprehensively the prevention and treatment of skin aging. The cosmetic composition of the present invention prevents skin aging through skin wrinkle improvement, skin elasticity improvement, and moisturizing ability by promoting fibroblast growth and collagen synthesis, and this fact is specified in the following examples. Therefore, anti-aging of the skin, which is the use of the present invention, is interpreted to include the use of wrinkle improvement, moisturizing improvement and skin elasticity improvement.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it is to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. Will be self-evident.

Preparation Example 1: Obtaining Camphor Ginseng Extract Using Various Extraction Solvents

Preparation Example 1-a

The camphor was washed with purified water and dried, 20 g of powdered camphor ginseng was added to 1.5 L of 70% ethanol solution, and the extract was heated for 4 hours in a reflux extractor equipped with a cooling condenser. Then, the obtained extract was filtered with a 300 mesh filter cloth, and the residue was extracted once more in the same manner, and then the respective extracts were combined and filtered again with a filter of Whatman 5, and the solvent was removed using a vacuum concentrator, Freeze drying yielded 2.74 g of dried ginseng extract.

Preparation Example 1-b

After washing the camphor ginseng with purified water and drying, 20 g of powdered camphor ginseng powder was added to 1.5 l of 1,3-butylene glycol solution, and the same procedure as in Preparation Example 1-a was carried out to extract 1.79 g of camphor ginseng dry weight. Obtained.

Preparation Example 1-c

20 g of powdered camphor ginseng was added to 1.5 L of acetone, followed by the same method as Preparation Example 1-a, to obtain 3.03 g of dried ginseng extract.

Preparation Example 1-d

Camphor ginseng was washed with purified water and dried, and then 20 g of powdered camphor ginseng powder was added to 1.5 L of purified water, and the same procedure as in Preparation Example 1-a was performed to obtain 1.72 g of dried ginseng extract.

Preparation Example 1-e

20 g of powdered camphor ginseng was added to 1.5 l of 70% methanol, followed by the same method as Preparation Example 1-a, to obtain a dry weight of 3.01 g of camphor ginseng.

Preparation Example 1-f

20 g of powdered camphor ginseng was added to 1.5 l of ethyl acetate solution, followed by the same method as Preparation Example 1-a, to obtain a dry weight of 2.65 g of camphor ginseng extract.

Preparation Example 1-g

20 g of powdered camphor ginseng was added to 1.5 l of chloroform solution, followed by the same method as Preparation Example 1-a, to obtain a dry weight of 2.49 g of camphor ginseng extract.

Preparation Example 1-h

20 g of powdered camphor ginseng was added to 1.5 L of normal butanol solution, followed by the same method as Preparation Example 1-a, to obtain a dry weight of 1.45 g of camphor ginseng extract.

Experimental Example 1 Fibroblast Proliferation Effect

Human normal fibroblasts (Korea Cell Line Bank) were seeded to 1 × 10 4 cells in each well of a 96-well microplate and incubated at 37 ° C. for 24 hours in DMEM medium (Sigma, USA). . Subsequently, each group of camphor ginseng extract prepared in Examples 1-a to 1-h was replaced with serum-free DMEM medium containing a final concentration of 100 μg / ml and serum-free DMEM medium without camphor ginseng extract Controls replaced with were further incubated for 24 hours. Then, 10 μl of MTT solution (3- (4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide: 5 mg / ml) was added to each, and incubated for 4 hours. The medium was then removed, shaken for 20 minutes by addition of 100 μl of dimethyl sulfoxide solution per well, and the absorbance of the supernatant was measured at 570 nm using a microplate reader (Molecular Devices, USA). . The measured values were substituted into the following Equation 1 to calculate the cell proliferation effect, and the results are shown in Table 1 below.

extract Absorbance Cell proliferation effect (%) Control 0.324 - Preparation Example 1-a 0.422 37.3 Preparation Example 1-b 0.413 27.5 Preparation Example 1-c 0.402 24.1 Preparation Example 1-d 0.431 33.1 Preparation Example 1-e 0.393 21.5 Preparation Example 1-f 0.445 30.3 Preparation Example 1-g 0.389 20.1 Preparation Example 1-h 0.400 23.5

As can be seen in Table 1, it can be seen that the fibroblast proliferation effect of about 20-30% at 100 ㎍ / ㎖ concentration of camphor ginseng extract compared to the control group not treated.

Experimental Example 2: Effect of Cell Proliferation According to the Concentration of Camphor Ginseng Extract

Except for using the camphor ginseng extract prepared in Preparation Example 1-a, which was confirmed to have the best fibroblast proliferation effect in Experimental Example 1, 50, 100 or 200 ㎍ / ㎖, cells in the same manner as Experimental Example 1 The proliferative effect was measured and the results are shown in Table 2 below.

Concentration (μg / ml) Absorbance (570 nm) Cell proliferation effect (%) Control 0.324 - 50 0.424 31.0 100 0.484 49.5 200 0.537 65.7

As can be seen in Table 2, it can be seen that the camphor ginseng extract promotes the growth of fibroblasts in a concentration-dependent manner, and also the results in Experimental Example 1 are significant.

Experimental Example 3: Collagen Synthesis Enhancement Effect

Human normal fibroblasts (Korea Cell Line Bank) were seeded to 2 x 10 4 cells in each well of a 96-well microplate and incubated for 24 hours at-° C in DMEM medium. Subsequently, the camphor ginseng extract prepared in Example 1-a was replaced with a serum-free DMEM medium containing no serum containing DMEM medium containing a final concentration of 50, 100 or 200 μg / ml, and a serum-free DMEM medium containing no camphor extract. One control was further incubated for 24 hours, ascorbic acid (50 g / ml) was added to promote collagen synthesis, followed by another 24 hours. Each well was then washed and replaced with serum-free DMEM medium and incubated for an additional 24 hours. After incubation, the supernatant of each well was collected and the amount of newly synthesized collagen was measured using a kit (Takara, Japan) for the amount of procollagen (procollagen) type I C-peptide (PICP). PICP amount was converted to ng / 2 × 10 4 cells and the results are shown in Table 3 below.

Concentration (μg / ml) Collagen Synthesis (ng / 2 x 104) Control 147 50 183 100 203 200 237

As can be seen in Table 3, it can be seen that the synthesis of collagen increases as the concentration of camphor ginseng extract increases, and thus, the camphor ginseng extract exhibits an excellent collagen synthesis enhancing effect.

Examples and Comparative Examples

To the flexible cosmetic composition having a composition ratio of the following Table 4 to prepare an example containing 0.6 wt% camphor ginseng extract prepared in Preparation Example 1-a and a comparative example not containing the camphor ginseng extract, used in Experimental Example 4 below Was:

ingredient Content (% by weight) glycerin 5.1 Propylene glycol 4.2 Tocopheryl Acetate 3.0 Liquid paraffin 4.6 Triethanolamine 1.0 Squalane 3.1 Macadamia Nut Oil 2.5 Polysorbate 60 1.6 Sorbitan sesquirrolate 1.6 Propylparaben 0.6 Carboxyl Vinyl Polymer 1.5 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Experimental Example 4: Moisturizing Effect

In order to evaluate the moisturizing power of each cosmetic prepared in the above Examples and Comparative Examples, the moisture retention capacity and the water evaporation amount of the skin to which each cosmetic was applied were measured as follows:

Experimental Example 4-1: Measurement of Moisture Retention Capacity

In the constant temperature and humidity room of 22 ° C. and 45% RH, each cosmetic prepared in Examples and Comparative Examples was coated with a predetermined amount (0.03 g / 16 cm 2) in the inner half of 30 test subjects, before application and after 1 hour of application. And moisture content of the skin 2 hours after application was measured, and normal skin without any treatment was used as a control. As a measuring device, a moisture content meter (corneometer CM820, Conrage + Khazaka, Germany) was used to measure the moisturizing power by measuring the electric capacity of the skin according to the moisture content of the skin. The results are shown in Table 5 below.

division Example Comparative Example Control Skin electrical conductivity before application 50 50 50 Skin electrical conductivity 1 hour after application 85 77 50 Skin electrical conductivity 2 hours after application 75 61 50

Experimental Example 4-2: Measurement of Water Evaporation Amount

After tape stripping on the upper arm of the arm to weaken the skin's protective film, apply the cosmetics to the subject in the same manner as in Experiment 4-1, and then use a moisture evaporator (TEWA meter, koln, Germany). Federated water evaporation was measured 5 times at 1 minute intervals every 12 hours using the Federal Bureau of Laboratories. The results are shown in Table 6 below.

division Example Comparative Example Control Moisture evaporation after 0 hours 4.0% 4.0% 0.0% Water evaporation after 12 hours -8.1% -4.5% -0.3% Water evaporation after 24 hours -7.6% -0.9% 0.0% Water evaporation after 36 hours -4.9%  2.1% 0.1% Water evaporation after 48 hours -2.1%  2.9% -0.2%

As shown in the results of Experimental Examples 4-1 and 4-2, the cosmetics of the examples of the present invention had higher electrical conductivity of the skin and less water evaporation amount than the cosmetics of the comparative examples. Therefore, it can be seen that the cosmetic of the present invention exhibits an excellent moisturizing effect.

Experimental Example 5: Skin irritation test

In order to determine the skin irritation of each camphor ginseng extract prepared according to the preparation example, a predetermined amount (about 0.1 g) of each sample at 5 × 20 cm in the lower arm of both arms for 30 men and women healthy adults 24 hours After patching for 1 hour and 24 hours after the removal, the skin condition change was visually read according to the following criteria, and the stimulation rate was calculated according to the following equation. The experimental results are shown in Table 7 below.

Criteria

-: No erythema or unusual phenomenon

+-: Slightly reddish

+: Significantly redder than the surroundings

++: reddens and swells more heavily

Production Example Judgment result Stimulation rate (%) ++ + +- - 1-a - - One 29 1.1 1-b - - One 29 1.1 1-c - - 2 28 2.2 1-d - - 2 28 2.2 1-e - - One 29 1.1 1-f - - 3 27 3.3 1-g - - 2 28 2.2 1-h - - One 29 1.1

As can be seen from the skin irritation test results of Table 7, the camphor ginseng extract of the present invention can be seen that there is no skin irritation.

Formulation Example 1

Formulation examples of the flexible lotion (skin) in the cosmetic composition containing the camphor ginseng extract obtained in Example 1-a is as follows:

ingredient Content (% by weight) Camphor Extract 0.5 1,3-butylene glycol 5.2 Oleyl alcohol 1.5 ethanol 3.2 Polysorbate 20 3.2 Benzophenone-9 2.0 Carboxyl Vinyl Polymer 1.0 glycerin 3.5 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 2

Formulation example of the milk lotion in the cosmetic composition containing the camphor ginseng extract obtained in Example 1-a is as follows:

ingredient Content (% by weight) Camphor Extract 0.6 glycerin 5.1 Propylene glycol 4.2 Tocopheryl Acetate 3.0 Liquid paraffin 4.6 Triethanolamine 1.0 Squalane 3.1 Macadamia Nut Oil 2.5 Polysorbate 60 1.6 Sorbitan sesquirrolate 1.6 Propylparaben 0.6 Carboxyl Vinyl Polymer 1.5 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 3

Formulation example of the nutrient cream in the cosmetic composition containing the camphor ginseng extract obtained in Example 1-a is as follows:

ingredient Content (% by weight) Camphor Extract 1.0 glycerin 4.0 vaseline 3.5 Triethanolamine 2.1 Liquid paraffin 5.3 Squalane 3.0 Beeswax 2.6 Tocopheryl Acetate 5.4 Polysorbate 60 3.2 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 3.1 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 4

An example of the formulation of the massage cream in the cosmetic containing the camphor ginseng extract of the present invention is as follows:

ingredient Content (% by weight) Camphor Extract 0.5 glycerin 4.0 vaseline 3.5 Triethanolamine 0.5 Liquid paraffin 24.0 Squalane 3.0 Beeswax 2.1 Tocopheryl Acetate 0.1 Polysorbate 60 2.4 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 2.3 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 5

Formulation examples of the pack in the cosmetic composition containing the camphor ginseng extract obtained in Example 1-a is as follows:

ingredient Content (% by weight) Camphor Extract 1.0 Ethyl alcohol 3.0 EDTA-2Na 0.02 Propylene glycol 5.1 Glyceline 4.5 Cabopol 1.0 Polyoxide 0.1 antiseptic a very small amount incense a very small amount Purified water Remaining amount system 100

As described in detail above, the present invention provides a cosmetic composition for preventing skin aging. As the cosmetic composition of the present invention further comprises a camphor ginseng extract in the conventional cosmetics, it promotes the proliferation of fibroblasts and collagen synthesis, improves the skin's moisturizing ability, and is very effective in preventing skin aging, and irritation to the skin. Because of this very small, it is very useful as a cosmetic composition for preventing skin aging without side effects on the skin.

Claims (6)

Camphor ginseng extract obtained by (a) anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, (b) acetone, (c) ethyl acetate, (d) chloroform or (e) 1,3-butylene glycol Cosmetic composition for preventing skin aging by improving wrinkles and moisturizing to include as an active ingredient. The cosmetic composition for preventing skin aging according to claim 1, wherein the camphor extract is obtained by using ethanol as an extraction solvent. The cosmetic composition for preventing skin aging according to claim 1, wherein the amount of camphor extract is 0.0001-10.0% by weight based on the total cosmetic composition. The composition of claim 1 wherein the cosmetic composition consists of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Cosmetic composition for preventing skin aging, characterized in that it has a formulation selected from the group. Camphor ginseng extract obtained by (a) anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, (b) acetone, (c) ethyl acetate, (d) chloroform or (e) 1,3-butylene glycol Cosmetic composition for skin moisturizing comprising as an active ingredient. The cosmetic composition for moisturizing skin according to claim 1, wherein the amount of camphor ginseng extract is 0.0001-10.0 wt% based on the total cosmetic composition.