KR20070096279A - A cosmetic composition comprising rumex crispus l. extracts - Google Patents

Abstract

A cosmetic composition comprising the extract of Rumex crispus L. is provided to prevent skin aging by promoting skin cell growth and collagen biosynthesis, inhibiting activity of elastase and increasing skin immunity, and moisturize the skin without side effects. A cosmetic composition for preventing skin aging and moisturizing the skin comprises 0.0001 to 20.0 wt.% of the extract of Rumex crispus L. which is prepared by extracting Rumex crispus L. with a solvent selected from water, C1-C4 anhydrous or hydrous lower alcohol including methanol, ethanol, propanol and butanol, a mixed solvent thereof, acetone, ethyl acetate, chloroform or 1,3-butyleneglycol. The cosmetic composition is formulated as solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation or spray.

Description

A Cosmetic Composition Comprising Rumex crispus L. Extracts

The present invention relates to a cosmetic composition containing the extract, and more particularly to a skin anti-aging cosmetic composition containing the extract as an active ingredient. The composition is not only less irritating to the skin, but also promotes skin cell proliferation, promotes collagen biosynthesis, inhibits elastase activity, or enhances skin immunity. In another aspect, the present invention relates to a skin moisturizing cosmetic composition containing the extract as an active ingredient, the composition exhibits a skin moisturizing enhancement effect.

The skin is the largest organ in our body, occupying about 16% of the body's volume, and is directly in contact with the outside environment, leaving many humans with external stimuli such as temperature, humidity, and ultraviolet radiation to enter the body. Serves as an important shield to protect. However, as we age, our skin is damaged by various contaminants, strong UV rays, stress and nutritional deficiencies, which damage skin cells and prevent cell proliferation. , Elasticity loss, keratinization, and the like. Originally, normal healthy skin has a defense system that protects the skin from these external damaging factors, but the persistent external stimulus loses the protective effect of protecting the skin from these external damaging factors, and the ability to protect and proliferate the cells. Falling, skin aging phenomenon such as decreased skin elasticity and wrinkles appear. In addition, the decrease in the moisturizing power of the skin, the slowing down of the skin keratinization rate is also a phenomenon due to skin aging. That is, as age increases, the amount of collagen, mucopolysaccharide and hyaluronic acid in the dermis matrix, which play an important role in maintaining skin elasticity, decreases and the amount of insoluble collagen increases. The dermis becomes difficult to keep the skin hydrated, and the skin becomes rough. In addition, the function of the sebaceous glands and sweat glands is reduced, and the amount of sebum and sweat is gradually reduced, and the sebaceous membrane is not properly formed, and thus the protection for skin moisturization is lost, resulting in dry skin and translucence. Loss, the elasticity of skin proteins decreases and wrinkles are created. In other words, the loss of moisturizing of the skin is a major symptom of aging along with decreased skin elasticity and wrinkle formation and is closely related.

In addition, it is known that wrinkles are generated when human skin cells are exposed to ultraviolet rays (UVA, UVB) due to the generation of reactive oxygen species, which is a major cause of skin damage. Collagen and elastic fibers (elastin) present in the dermal layer of the skin structure is the main protein that controls the elasticity of the skin. Biosynthesis of collagen fiber is internally and externally nourishment of the skin. Internally, aging (reduction of estrogen, etc.) causes cell activity to decrease, collagen fiber decreases. External factors include active oxygen (free radical type) generated by excessive irradiation of UV rays and stress, which react with the -SH group of the protein to inhibit the activity of the enzyme, or decompose collagen and elastin (Matrix Metalloproteinases: MMPS) to increase collagenase expression. Meanwhile, elastin fibers, along with collagen fibers, are involved in skin elasticity through crosslinking in the dermis. With age, the skin increases histologically the activity of elastinase, an elastin degrading enzyme, causing aging, which reduces wrinkles and elasticity of the skin due to lack of elastin fibers.

Until now, in order to suppress or slow down the progression of the skin, cosmetics or skin protectants containing various skin protection ingredients such as retinol, retinoid, vitamin C, flavonoids and tocopherol have been developed. In the final formulation, the potency is low, the efficacy is mild, and the skin irritation has a serious problem.

Recently, due to the skin irritation problem of these raw materials, the development of cosmetics using natural vegetable raw materials that are safer and have no skin irritation have been made. In particular, in Korea, we are developing a lot of cosmetics using natural herbal ingredients. This is because natural herbal ingredients have been used in the private sector for a long time and safety and effectiveness have already been secured. Because there is.

     On the other hand, the extracts used in the present invention is an example used in the athlete's foot preventive and therapeutic cosmetic soap composition (Korean Patent Publication No. 1998-0068394), but the example used in the cosmetic composition for preventing skin aging or moisturizing skin none.

In the present invention, using the traditional herbal raw materials that have been used as a folk remedy for a long time, it was intended to develop a cosmetic composition for preventing skin aging that has no skin side effects and excellent skin improvement effect. The active ingredient was separated and applied to the product to develop natural herbal anti-aging cosmetics. Accordingly, the present inventors completed the present invention by confirming that the extract of Soru-Zi showed a complex anti-aging effect on the skin with little side effects, especially skin irritation.

Accordingly, it is an object of the present invention to provide a cosmetic composition for preventing skin aging.

In addition, another object of the present invention to provide a cosmetic composition for moisturizing the skin.

The present invention relates to a skin cosmetic composition containing an extract of Soru-Jin, and more particularly, to a cosmetic composition for preventing skin aging containing the extract of Soru-Ju as an active ingredient, and the composition is very irritating to the skin. In addition, it exhibits an effect of promoting skin cell proliferation, promoting collagen biosynthesis, inhibiting elastase activity, or enhancing skin immune system.

In addition, the present invention relates to a cosmetic composition for moisturizing skin containing Soru-Jin extract as an active ingredient, the composition exhibits a skin moisturizing enhancement effect.

Rumex cripus L. used as an active ingredient in the present invention is called a yangje-geun, sulpheun, muzzle, etc., is a perennial grass of the perennial herb that dries up in midsummer and grows again when cool. Young leaves are eaten as herbs, and are said to be effective for ringworm, athlete's foot, and eczema. Since ancient times, it has been used in skin diseases such as amelioration, scabies and skin cancer. It is called Yangje-geun when the roots are cracked and rolled up in the autumn and dried in the sun.

An extract of the active ingredient contained in the cosmetic composition of the present invention is dried by extracting the root of the extract. That is to say, the rubbing is used in various extraction solvents, for example, water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, propanol, butanol, etc.), a mixed solvent of the lower alcohol with water, acetone, ethyl acetate By using chloroform or 1,3-butylene glycol can be obtained extracts.

However, it will be apparent to those skilled in the art that the extract of the present invention is not limited to the above-described extraction solvent, but an extract of Soru-Zhich which exhibits substantially the same effect using other extraction solvents can be obtained.

On the other hand, the extract is characterized in that it contains 0.0001 to 20.0% by weight based on the total weight of the cosmetic composition. Preferably it is 0.0005 to 10.0 weight%, More preferably, it is 0.005 to 5.0 weight%. When the content of the rubbing extract is less than 0.0001% by weight, the effect is difficult to expect, and when it exceeds 20.0% by weight, there is no apparent increase in effect as the content increases.

The components included in the cosmetic composition of the present invention include components conventionally used in cosmetic compositions in addition to the extract from the extract as an active ingredient, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings, And carriers.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations and sprays, and the like, but are not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing composition, it is an aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide as a carrier component. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

On the other hand, the term "anti-aging of the skin" used in the specification of the present invention has a meaning encompassing the prevention and treatment of skin aging comprehensively, including the improvement of skin wrinkles and skin elasticity, etc. The purpose of prevention may be achieved.

Hereinafter, the present invention will be described in more detail with reference to Preparation Examples, Examples and Comparative Examples, but the present invention is not limited only to these examples. That is, these examples are only for illustrating the present invention in more detail, and the scope of the present invention is not limited by these examples in accordance with the gist of the present invention, those skilled in the art. Will be self-evident.

Production Example  One : Rumor  Preparation of Extract

Weigh 100 g of dried Soruja root and finely crush it using a crusher. Add 70% ethanol to make 0.7L, and mature it at 25 ℃ for 5 days. After filtering this, the precipitate is discarded and the filtrate is collected and distilled under reduced pressure. Dissolve 1 g of the residue in a 50% butylene glycol solution to make a 100 mL solution to use as a cosmetic composition.

Experimental Example  1: Proliferation effect of fibroblasts and keratinocytes

Human normal fibroblasts and keratinocytes (Korea Cell Line Bank) were inoculated to 1 x 10 ⁴ cells in each well of a 96-well microplate, and 37 ° C for 24 hours in DMEM medium (Sigma). Incubated at. Subsequently, the test group replaced with DMEM medium without serum contained in the extract of Soru-Jin in Preparation Example 1 at concentrations of 0.005%, 0.01%, 0.05%, 0.1%, and 1.0%, and serum without the extract Controls replaced with DMEM free medium were further incubated for 24 hours. Then, 10 μl of MTT solution (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide: 5 mg / ml) was added to each and incubated for 4 hours. The medium is then removed, shaken for 5 minutes by addition of 100 μl of dimethyl sulfoxide solution per well and the absorbance of the supernatant at 570 nm using a microplate reader (Molecular Devices, USA). It was. The measured values were substituted into the following Equation 1 to calculate the cell proliferation effect, and the results are shown in Tables 1 and 2 below.

Cell Proliferation Effects in Skin Fibroblasts ingredient(%) Cell proliferation effect (%) Control - Preparation Example 1 0.005 4.7 0.01 19.5 0.05 30.8 0.1 55.6 1.0 70.3

Cell proliferation effect in skin keratinocytes ingredient(%) Cell proliferation effect (%) Control - Preparation Example 1 0.005 4.2 0.01 19.3 0.05 34.2 0.1 46.9 1.0 70.8

As can be seen in Tables 1 and 2, it can be seen that the fibroblasts and keratinocytes showed a proliferative effect in the experimental group treated with the extract, while the extract was not treated with the extract.

Experimental Example  2: collagen synthesis promoting effect

Human normal fibroblasts (Korea Cell Line Bank) were seeded to 2 x 10 ⁴ cells in each well of a 96-well microplate and incubated for 24 hours at 37 ° C in DMEM medium. Subsequently, the test group replaced with DMEM medium without serum contained in the extract of Soru-Jin in Preparation Example 1 at a concentration of 0.005%, 0.01%, 0.05%, and 0.1%, and the serum-free DMEM medium without containing the extract The replaced control is further incubated for 24 hours.

At this time, TGF-beta (10ng / ml) was applied as a positive control. After incubation, the supernatant of each well was collected and the amount of newly synthesized collagen was measured using a procollagen type I C-peptide (PICP) kit (Takara, Japan). Collagen synthesis enhancing effect was obtained by the amount of collagen produced increased compared to the control.

Collagen biosynthesis promoting effect ingredient(%) Collagen Synthesis Enhancement Effect (%) Positive control 189.8 Preparation Example 1 0.005 105.7 0.01 126.3 0.05 141.8 0.1 167.5

As can be seen in Table 3, it can be seen that the synthesis of collagen increases as the concentration of the extract is increased, and it can be seen that the extract shows an excellent collagen synthesis enhancing effect.

Experimental Example  3: Elastase  Inhibitory effect

Elastase was extracted from porcine pancreas and used Sigma's. In the assay based on the hydrolysis of the synthetic peptide substrate by pancreatic elastase, the increase in the amount of 4-nitroaniline per unit time at light wavelength 410 nm was taken as the catalytic activity value of elastase. First, 1.0 mL of the substrate solution was placed in a test tube, and potassium phosphate buffer solution and distilled water were added thereto. 0.2 mL of the extract sample solution containing the extract of Soru-Ju in Preparation Example 1 in an ethanol solution at a concentration of 0.005%, 0.01%, 0.05%, and 0.1% was reacted for 10 minutes in a 37 ° C thermostat.

At this time, the control group was put in 0.2mL each solvent instead of the extract of Sorujeom and measured the absorbance at a wavelength of 410nm with a spectrophotometer. The elastase inhibitory effect according to the concentration of the extract was obtained by the following equation.

Elastase Inhibition Rate = [1- Enzyme Activity in Control / Enzyme Activity in Control] X 100

Elastase inhibitory effect ingredient(%) Elastase inhibitory effect (%) Control 0 Preparation Example 1 0.005 0.05 0.01 34.7 0.05 52.8 0.10 76.3

As can be seen in Table 4, it can be seen that the elastase inhibitory effect increases as the concentration of the extract is increased, and thus the extract exhibits an excellent elastase inhibitory effect.

Experimental Example  4: Skin immune enhancing efficacy test

After shaving the abdomen in 8-10-week-old C57BL / 6 mice, irradiation with ultraviolet B wavelength (UVB) of 2.4KJ / m ² using FS-20 sun lamp showed that the contact hypersensitivity was controlled. Compared to 44.3% suppressed immunosuppressive phenomenon will result. Therefore, after the UV irradiation, the concentrations of the extracts of the extracts of Preparation Example 1 of the present invention 1, 0.5%, 1.0%, and 5.0%, respectively, were sensitized with 3% trinitrochlorobenzene in the UV irradiation area after 3 days. Let's do it. After 6 days, 1% trinitrogen chloride was applied to the ears of mice and edema status was measured. Skin immune enhancing effect is calculated by the following formula and the results are shown in Table 5 below.

Skin Immuno-sensitizing Effect of Soru-Jin Extract ingredient(%) Skin immune sensitization effect (%) Control - Preparation Example 1 0.5 (+) 7.3 1.0 (+) 21.9 5.0 (+) 37.8

As can be seen in Table 5, it can be seen that the increase in skin immune response increases as the concentration of the extract is increased. Thus, it can be seen that the extract is excellent skin immunity increase effect.

[ Production Example  2]

To prepare a flexible cosmetics having a composition ratio of the following Table 6 containing the extract and the comparative example without the extract of Soru-Ju extract was used in Experimental Example 5 below:

Ingredient (% by weight) Example Comparative example Sorousum Extract 3.0 - glycerin 5.1 5.1 Propylene glycol 4.2 4.2 Tocopheryl Acetate 3.0 3.0 Liquid paraffin 4.6 4.6 Triethanolamine 1.0 1.0 Squalane 3.1 3.1 Macadamia Nut Oil 2.5 2.5 Polysorbate 60 1.6 1.6 Sorbitan sesquirrolate 1.6 1.6 Propylparaben 0.6 0.6 Carboxyl Vinyl Polymer 1.5 1.5 incense a very small amount a very small amount antiseptic a very small amount a very small amount Purified water to 100 to 100

Experimental Example  5: moisturizing effect

In order to evaluate the moisturizing power of each of the cosmetic preparations prepared in Examples and Comparative Examples, the moisture retention ability and the water evaporation amount of the skin to which each cosmetic was applied were measured as follows.

Experimental Example  5-1: Moisture Capacity  Measure

In the constant temperature and humidity room of 22 ° C. and 45% RH, each cosmetic prepared in Examples and Comparative Examples was coated with a predetermined amount (0.03 g / 16 cm 2) in the lower half of 30 test subjects, before application, after 1 hour of application, and The moisture content of the skin 2 hours after application was measured, and normal skin without any treatment was used as a control. The measuring device used a moisture content measuring instrument (Corneometer CM825, Courage + Khazaka, Germany) to measure the moisturizing power by measuring the electrical capacity of the skin according to the moisture content of the skin. The results are shown in Table 7 below.

division Example Comparative example Control Skin electrical conductivity before application 50 50 50 Skin electrical conductivity 1 hour after application 79 75 50 Skin electrical conductivity 2 hours after application 82 50 50

Experimental Example  5-2: Measurement of water evaporation amount

After applying tape stripping to the upper arm of the arm to weaken the skin's protective film, apply the cosmetics to the test subjects in the same manner as in Experiment 5-1, and then use a moisture evaporator (Tewameter TM300, Courage + Khazaka). Co., Ltd., Germany), the water evaporation amount was measured 5 times at 1 minute intervals every 12 hours, and the average value was obtained. The results are shown in Table 8 below.

division Example Comparative example Water evaporation after 12 hours -9.8% -4.5% Water evaporation after 24 hours -7.2% -2.0% Water evaporation after 36 hours -4.5%  3.5% Water evaporation after 48 hours -2.1%  4.0%

As shown in the results of Experimental Examples 5-1 and 5-2, the cosmetics of the Examples of the present invention had a higher electrical conductivity of the skin and a smaller amount of water evaporation than the cosmetics of the Comparative Examples. Therefore, it can be seen that the cosmetic of the present invention exhibits an excellent moisturizing effect.

Experimental Example  6: skin wrinkle improvement effect on human skin

Wrinkle improvement effect on the cosmetic composition containing Soru-Ji extract was measured in 15 healthy Korean women. The subjects with facial wrinkles aged 33 to 51 years were compared and evaluated for improving skin wrinkles of the cosmetics prepared in Examples and Comparative Examples. An example was used for the face left part of the subject, and a comparative example was used for the right part for 2 months. After measuring the skin condition on both sides of the face before use of the composition, the same site was remeasured two months after use to measure changes in skin wrinkles. The skin was measured in a constant temperature and humidity room of 22 ° C. and 40% relative humidity. Wrinkles at the tail of the eyes were replicated, and skin wrinkles were measured with a skin visometer (Skin Visiometer SV400, Electronics GmbH., Germany). The amount of change in skin wrinkles was calculated according to Equation 4 below.

Change Amount (△%) = {(Tdi-Td0) / Tdo} X 100

          Tdi; Measured value at D60

          Tdo; Measured value at D0

As a result of calculation according to the above formula, the skin wrinkle at the site using the comparative example showed a decrease of 2.8 ㅁ 1.5% (mean ㅁ standard deviation), whereas the skin wrinkle at the site using the example showed a decrease of 11.9 ㅁ 3.4%. Excellent skin wrinkle improvement. According to the above results, it can be confirmed that the cosmetic composition containing the rubbing extract has a great influence on skin wrinkle improvement.

Formulation example  One

Formulation examples of the softening longevity (skin) in the cosmetics containing the extract obtained by the above-mentioned Soru-Jung is as follows.

ingredient Content (% by weight) Sorousum Extract 0.5 1,3-butylene glycol 5.2 Oleyl alcohol 1.5 ethanol 3.2 Polysorbate 20 3.2 Benzophenone-9 2.0 Carboxyl Vinyl Polymer 1.0 glycerin 3.5 incense a very small amount antiseptic a very small amount Purified water to 100

Formulation Example 2

Formulation example of the milk lotion in the cosmetic composition containing the extract obtained by the above-mentioned Soru-Jung is as follows.

ingredient Content (% by weight) Sorousum Extract 0.6 glycerin 5.1 Propylene glycol 4.2 Tocopheryl Acetate 3.0 Liquid paraffin 4.6 Triethanolamine 1.0 Squalane 3.1 Macadamia Nut Oil 2.5 Polysorbate 60 1.6 Sorbitan sesquirrolate 1.6 Propylparaben 0.6 Carboxyl Vinyl Polymer 1.5 incense a very small amount antiseptic a very small amount Purified water to 100

Formulation Example 3

An example of the formulation of the nourishing cream in the cosmetic containing the extract obtained from the above-mentioned Soru-Ji is as follows.

ingredient Content (% by weight) Sorousum Extract 1.0 glycerin 4.0 vaseline 3.5 Triethanolamine 2.1 Liquid paraffin 5.3 Squalane 3.0 Beeswax 2.6 Tocopheryl Acetate 5.4 Polysorbate 60 3.2 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 3.1 incense a very small amount antiseptic a very small amount Purified water to 100

Formulation example  4

An example of the formulation of the massage cream in the cosmetic containing the extract of Soru-Jin of the present invention is as follows.

ingredient Content (% by weight) Sorousum Extract 0.5 glycerin 4.0 vaseline 3.5 Triethanolamine 0.5 Liquid paraffin 24.0 Squalane 3.0 Beeswax 2.1 Tocopheryl Acetate 0.1 Polysorbate 60 2.4 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 2.3 incense a very small amount antiseptic a very small amount Purified water to 100

Formulation Example 5

Formulation examples of the pack in the cosmetic containing the extract obtained from the above-mentioned Sorujeung is as follows.

ingredient Content (% by weight) Sorousum Extract 1.0 Ethyl alcohol 3.0 EDTA-2Na 0.02 Propylene glycol 5.1 Glyceline 4.5 Cabopol 1.0 Polyoxide 0.1 antiseptic a very small amount incense a very small amount Purified water to 100

As described above, the present invention provides a cosmetic composition containing the extract of Soru-Jin. In particular, by containing the extract sorujeuk provides a cosmetic composition for preventing skin aging through the proliferation of fibroblasts, keratinocyte growth, collagen synthesis promotion or elastase enzyme inhibitory effect. In addition, it is also useful as a cosmetic composition for moisturizing the skin by improving the moisturizing power of the skin. In addition, the composition is very useful as a cosmetic composition having no side effects on the skin because it is very irritating to the skin.

Claims (6)

Skin anti-aging cosmetic composition containing the extract as an active ingredient.        The cosmetic composition according to claim 1, wherein the composition is characterized in that fibroblast proliferation, proliferation of skin keratinocytes, promotion of collagen synthesis, inhibition of elastase enzyme, or enhancement of skin immunity. Skin moisturizing cosmetic composition containing the extract as an active ingredient. The method according to any one of claims 1 to 3, wherein the rubbing extractRumex cripus L.Cosmetic composition characterized in that. The cosmetic composition according to any one of claims 1 to 3, wherein the extract is 0.0001 to 20.0% by weight based on the total weight of the cosmetic composition. The composition of claim 1, wherein the composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion emulsion, Cosmetic composition, characterized in that it has a formulation selected from the group consisting of wax foundation and spray.