KR100773863B1 - Manufacturing Method and Cosmetic Compositions Preventing Skin Aging - Google Patents

Abstract

The present invention relates to a cosmetic composition, and more specifically, Lithospermun erythrorhizon, Golden (Scutellaria baicalensis Geogri), Lacquer (Plagiorhegma dubium Maxim), Magnolia ovobata Thunb, Phellinus linteus (Phellinus linteus) extract The present invention relates to a cosmetic composition for preventing skin aging and a method of manufacturing the same by complex acting as an anti-wrinkle, elasticity enhancing, and moisturizing effect, including the present invention.

[Cosmetic compositions comprising conventional adjuvants and carriers such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings, based on 500 ml of distilled water, 5 g of licorice, 10 g of golden gourd, 5 g of gold, 2 g of yellow lotus, 2 g of mushrooms Contains a herbal extract extracted and prepared at a compounding ratio,

The herbal extract is 0.0001-10.0% by weight relative to 100% by weight of the total cosmetic composition,

The cosmetic composition is any one selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays. Having the formulation of

In the method of manufacturing a cosmetic composition comprising a carrier and conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances, 5 g of licorice, 10 g of golden gourd, 5 g of gold, 2 g of yellow lotus, 2 g of mushrooms After finely crushed by using 500ml of distilled water, and then decoction for 4 hours at 50 ℃ and filtered and then cooled at room temperature and then aged for 2 days at low temperature and filtered to produce a herbal extract prepared by By constructive features,

The cosmetic composition of the present invention is not irritating to the skin, promotes cell proliferation in skin cells, and has the characteristics of promoting collagen biosynthesis, antioxidant, anti-inflammatory effect, and moisturizing effect, thus preventing skin aging and improving atopic skin. Very useful.

Cosmetics, skin aging, soybeans, thick skin, mushrooms, golden, yellow, moisturizing, collagen, fibroblast, antioxidant, anti-inflammatory,

Description

Cosmetic composition for preventing skin aging and a method of manufacturing the same {Manufacturing Method and Cosmetic Compositions Preventing Skin Aging}

     The present invention relates to a cosmetic composition, and more particularly, to the present cosmetics (Lithospermun erythrorhizon), Golden (Scutellaria baicalensis Geogri), Nasturtium (Plagiorhegma dubium Maxim), Magnolia ovobata Thunb, Phellinus mushroom (Phellinus linteus) extract It relates to a cosmetic composition and a method of manufacturing the same, including the active ingredient, such as wrinkle improvement, elasticity enhancement and moisturizing enhancement effect to include the active ingredient to prevent skin aging, atopic skin improvement and the like.

The skin is the largest organ of our body and occupies about 16% of our body volume. It is in direct contact with the external environment, and many of the deadly harmful factors that try to penetrate the body. It plays an important role in protecting the human body from.

However, as we age, our skin is damaged by natural aging, various contaminants, strong ultraviolet rays, stress and nutritional deficiencies. Skin aging may occur such as wrinkle formation, loss of elasticity and keratinization. In addition, the decrease in the moisturizing power of the skin, the slowing down of the skin keratinization rate is also a phenomenon due to skin aging. That is, as age increases, the amount of collagen, mucopolysaccharide, and hyaluronic acid in the dermis matrix plays an important role in maintaining skin elasticity, and the amount of insoluble collagen increases. The dermis becomes difficult to keep the skin hydrated, and the skin becomes rough. In addition, the function of sebaceous glands and sweat glands is reduced, and the amount of sebum and sweat is gradually reduced, and the sebaceous membrane is not properly formed, and thus the protection function for the skin is lost. Eventually, the skin becomes dry and translucence. Loses skin elasticity and wrinkles. In other words, the loss of moisturizing of the skin is a major symptom of aging along with decreased skin elasticity and wrinkle formation and is closely related.

In addition, it is known that wrinkles are generated when human skin cells are exposed to ultraviolet rays (UV-B, UV-A) due to the generation of reactive oxygen species, which is a major cause of skin damage. Collagen and elastic fibers (elastin) present in the dermal layer of the skin structure is the main protein that controls the elasticity of the skin. Biosynthesis of collagen fiber is internally and externally nourishment of the skin. Internally, aging (reduction of estrogen) causes cell activity to decrease and collagen fiber decreases. External factors include active oxygen (free radical type) generated by excessive irradiation of UV rays and stress, which react with the -SH group of the protein to inhibit the activity of the enzyme, or decompose collagen and elastin (Matrix Metalloproteinases: MMPS) increases the expression of collagenase, causing aging that reduces wrinkles and elasticity of the skin.

Until now, in order to suppress or slow down the progression of skin, cosmetics or skin protectants containing various skin protection ingredients such as retinol, retinoid, vitamin C, flavonoids, and tocopherol have been developed. In the final formulation, the potency is low, the efficacy is mild, and the skin irritation has a serious problem.

Recently, due to the skin irritation problem of these raw materials, the development of cosmetics using natural vegetable raw materials that are safer and have no skin irritation have been made. In particular, in Korea, we are developing a lot of cosmetics using natural herbal ingredients. This is because natural herbal ingredients have been used in private for a long time and safety and effectiveness have already been secured. Because there is.

In the present invention, by using a traditional herbal raw material that has been used for a long time there is no skin side effect and to develop a cosmetic composition for preventing skin aging excellent skin improvement effect,

In order to solve the above-mentioned side effects by using the licorice, scabbard, golden, sulfur, mushrooms, in particular, the present invention is suitable for sensitive skin, such as atopic dermatitis, and exhibits complex anti-aging effect with little skin irritation The purpose is to provide.

Specific solution means for achieving the above object of the present invention,

[Cosmetic compositions comprising conventional adjuvants and carriers such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings, based on 500 ml of distilled water, 5 g of licorice, 10 g of golden gourd, 5 g of gold, 2 g of yellow lotus, 2 g of mushrooms Contains a herbal extract extracted and prepared at a compounding ratio,

The herbal extract is 0.0001-10.0% by weight relative to 100% by weight of the total cosmetic composition,

The cosmetic composition is any one selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays. Having the formulation of

In the method of manufacturing a cosmetic composition comprising a carrier and conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances, 5 g of licorice, 10 g of golden gourd, 5 g of gold, 2 g of yellow lotus, 2 g of mushrooms After finely crushed by using 500ml of distilled water, and then decoction for 4 hours at 50 ℃, filtered and then cooled at room temperature and then matured at low temperature for 2 days and filtered to produce a herbal extract prepared by the composition] The above object can be attained by the characteristic feature.

Hereinafter, the specific configuration of the present invention,

Ingredients included in the cosmetic composition of the present invention include ingredients commonly used in cosmetic compositions in addition to herbal extracts as active ingredients, and include, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings, and Including a carrier,

In the cosmetic composition comprising the usual adjuvants and carriers such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings, the blending ratio of 5 g of licorice, 10 g thick, 5 g golden, 2 g rhubarb, 2 g of mushrooms based on 500 ml of distilled water Herbal extracts prepared by extraction are included.

The present invention as described above may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations and sprays, and the like, but are not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

The cosmetic composition of the present invention has the use of anti-aging of the skin, and the term "anti-aging of the skin" in this specification has the meaning encompassing comprehensively the prevention and treatment of skin aging.

The cosmetic composition of the present invention prevents skin aging through skin wrinkle improvement, skin elasticity improvement, and moisturizing ability by promoting fibroblast growth and collagen synthesis, and this fact is specified in the following examples.

Therefore, anti-aging of the skin, which is the use of the present invention, is interpreted to include the use of wrinkle improvement, moisture retention, and skin elasticity improvement.

Hereinafter, the present invention will be described in more detail with reference to the following examples. These examples are only for illustrating the present invention in more detail, and the scope of the present invention is not limited by these examples in accordance with the gist of the present invention to those skilled in the art. Will be self-evident.

Production Example  1: Preparation of herbal extract

Licorice 5g, 10g thick, golden 5g, rhubarb 2g, 2g of mushrooms, finely crushed by using a crusher, and then added to 500ml of distilled water, and this is about 4 hours at 50 ℃. This is filtered again, cooled to room temperature, then aged at low temperature for 2 days and then filtered.

In a preferred embodiment of the present invention, the content of the herbal extract is 0.0001 to 20.0% by weight relative to 100% by weight of the total cosmetic composition, more preferably 0.0005 to 10.0% by weight, most preferably 0.005 to 5.0% by weight . If the content of the herbal extract is less than 0.0001% by weight, it is not expected to have a clear skin anti-aging effect, in particular, wrinkle improvement. In addition, when the content of the herbal extracts exceeds 10.0% by weight, there is no apparent increase in effect as the content increases.

Experimental Example  1: proliferative effect of fibroblasts and keratinocytes

Human normal fibroblasts and keratinocytes (Korea Cell Line Bank) were inoculated to 1 x 10 ⁴ cells in each well of a 96-well microplate, and in DMEM medium (sigma) for 24 hours- Incubated at 37 ° C.

Subsequently, the experimental group was replaced with a serum-free DMEM medium containing the final concentration of 0.005%, 0.01%, 0.05%, 0.1%, 1% and the control group replaced with a serum-free DMEM medium containing no herbal extract. Was further incubated for 24 hours.

Then, 10 μl of MTT solution (3- (4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide: 5 mg / ml) was added to each, and incubated for 4 hours. The medium was then removed, shaken for 20 minutes by addition of 100 μl of dimethyl sulfoxide solution per well, and the absorbance of the supernatant was measured at 570 nm using a microplate reader (Molecular Devices, USA). . The measured values were substituted into the following Equation 1 to calculate the cell proliferation effect, and the results are shown in Table 1 below.

                      Extract Treatment Absorbance-Control Absorbance

Cell proliferation effect = --------------------------------------------- X 100

                               Control absorbance

Cell Proliferation Effects in Skin Fibroblasts Extract (%) Cell proliferation effect (%) Control              - 0.005     7.8 0.01     12.4 0.05     26.6 0.1     40.5 1.0      58.9

Cell proliferation effect in skin keratinocytes   Extract (%)  Cell proliferation effect (%)   Control   -    0.005   5.3    0.01   15.2    0.05   29.7    0.1   43.7    1.0   61.4

As can be seen in Tables 1 and 2, it can be seen that the herbal extract shows a proliferative effect of fibroblasts and keratinocytes compared to the control group not treated with the herbal extract.

Experimental Example  2: collagen synthesis promoting effect

Human normal fibroblasts (Korea Cell Line Bank) were seeded in each well of a 96-well microplate to 2 × 10 ⁴ cells and incubated for 24 hours at −37 ° C. in DMEM medium.

Subsequently, the experimental group in which the prepared herbal extracts were replaced with serum-free DMEM medium containing final concentrations of 0.005, 0.01, 0.05, and 0.1% and the control group replaced with serum-free DMEM medium containing no herbal extracts for 24 hours. Incubate further. At this time, TGF-beta (10ng / ml) was applied as a positive control.

After incubation, the supernatant of each well was collected and the amount of newly synthesized collagen was measured using a kit (Takara, Japan) for the amount of procollagen (procollagen) type I C-peptide (PICP). Collagen synthesis enhancing effect was calculated using the amount of collagen produced and increased compared to the control.

%, V / v   Collagen Synthesis Enhancement Effect (%)   TGF-b   180.7   0.005   112.6   0.01   134.9   0.05   156.3   0.10   175.7

As can be seen in Table 3, it can be seen that the synthesis of collagen increases as the concentration of the herbal extracts increases, and thus the herbal extracts show an excellent collagen synthesis enhancing effect.

Experimental Example  3: skin immune enhancing effect test

After shaving the abdomen in 8 to 10 week old C57BL / 6 mice, ultraviolet B wavelength (UVB) of 2.4KJ / m2 using FS-20 sun lamp was applied to control hypersensitivity In comparison, 44.3% of the immunosuppression is caused. Therefore, the herbal extract of the present invention is applied after UV irradiation, and after 3 days, sensitized with 3% trinitrochlorobenzene in the UV irradiation area. After 6 days, 1% trinitrogen chloride was applied to the ears of mice and edema status was measured. The skin immune enhancing effect is calculated by the following Equation 2 and the results are shown in Table 4 below.

Skin Immune Enhancement Effect

Application and ear edema thickness of UVB irradiated group-ear edema thickness of UVB irradiated group

-------------------------------------------------- ------------ X 100

Ear edema thickness in the control group-Ear edema thickness in the UVB irradiation group

Skin Immune Effect of Herbal Extracts   %, V / v   Skin immune enhancing effect (%)   Control   -   0.5   (+) 5.3   1.0   (+) 13.7   5.0   (+) 35.8

Example  And comparison Example

To prepare a flexible herbal cosmetic composition having a composition ratio of the following Table 5 Example 3 containing the herbal extract 3% (v / v) and Comparative Examples without the herbal extract was prepared, used in Experimental Example 4 below. :

Cosmetics containing herbal extracts     ingredient   Example (Content; Weight%) Comparative Example (Content; Weight%)  Herbal Extract 3.0  -  glycerin 5.1 5.1  Propylene glycol 4.2 4.2  Tocopherol Acetate 3.0 3.0  Liquid paraffin 4.6 4.6 Triethanolamine 1.0 1.0  Squalane 3.1 3.1  Macadamia Nut Oil 2.5 2.5  Polysorbate 60 1.6 1.6  Sorbitan 1.6 1.6  Propylparaben 0.6 0.6  Kabapol 1.5 1.5  incense a very small amount a very small amount  antiseptic a very small amount a very small amount  Purified water Remaining amount Remaining amount  system 100 100

Experimental Example  4: moisturizing effect

In order to evaluate the moisturizing power of each cosmetic prepared in the above Examples and Comparative Examples, the moisture retention capacity and the water evaporation amount of the skin to which each cosmetic was applied were measured as follows.

Experimental Example  4-1: Moisture Capacity  Measure

In the constant temperature and humidity room of 22 ° C. and 45% RH, each cosmetic prepared in Examples and Comparative Examples was coated with a predetermined amount (0.03 g / 16 cm 2) in the inner half of 30 test subjects, before application and after 1 hour of application. And moisture content of the skin 2 hours after application was measured, and normal skin without any treatment was used as a control. The measuring device used a moisture content measuring instrument (corneometer CM820, Conrage + Khazaka, Germany) to measure the moisturizing power by measuring the electric capacity of the skin according to the moisture content of the skin. The results are shown in Table 6 below.

      division Example Comparative Example Control Skin electrical conductivity before application 50 50 50 Conductivity 1 hour after application 75 70 50 Conductivity 2 hours after application 69 43 50

Experimental Example  4-2: Measurement of Water Evaporation Amount

After tape stripping on the upper arm of the arm to weaken the skin's protective film, apply the cosmetics to the subject in the same manner as in Experiment 4-1, and then use a moisture evaporator (TEWA meter, koln, Germany). Federated water evaporation was measured 5 times at 1 minute intervals every 12 hours using the Federal Bureau of Laboratories. The results are shown in Table 7 below.

division Example Comparison Control Moisture evaporation after 0 hours 6.0% 5.0% 0.0% Water evaporation after 12 hours -12.1% -5.4% -0.2% Water evaporation after 24 hours -9.8% -3.6% 0.1% Water evaporation after 36 hours -4.8% 3.6% 0.1% Water evaporation after 48 hours -3.7% 4.9% 0.0%

As shown in the results of Experimental Examples 4-1 and 4-2, the cosmetics of the examples of the present invention had higher electrical conductivity of the skin and less water evaporation amount than the cosmetics of the comparative examples. Therefore, it can be seen that the cosmetic of the present invention exhibits an excellent moisturizing effect.

Formulation example  One

Formulation examples of the flexible lotion (skin) in the cosmetic composition containing the herbal extract obtained above is as follows.

ingredient Content (% by weight)   Herbal extract 0.5 1,3-BG 5.2 Oleyl alcohol 1.5 ethanol 3.2 Polysorbate 20 3.2 Benzophenone-9 2.0 Kabapol 1.0 glycerin 3.5 incense a very small amount antiseptic a very small amount Purified water Remaining amount   system 100

Formulation example  2

Formulation examples of the milk lotion in the cosmetic containing the herbal extract obtained above are as follows.

ingredient Content (% by weight)  Herbal extract 0.6  glycerin 5.1  Propylene glycol 4.2  Tocopheryl Acetate 3.0  Liquid paraffin 4.6  Triethanolamine 1.0 Squalane 3.1  Macadamia Nut Oil 2.5 Polysorbet 60 1.6  Sorbitan sesquinolate 1.6  Propylparaben 0.6 Kabapol 1.5  incense a very small amount  antiseptic a very small amount  Purified water Remaining amount  system 100

Formulation example  3

Formulation example of the nutrient cream in the cosmetic containing the herbal extract obtained above is as follows.

ingredient Content (% by weight)  Herbal extract 2.0  glycerin 4.0  vaseline 3.5  Triethanol amine 2.1  Floating paraffin 5.3  Squalane 3.0  Beeswax 2.6  Tocopheryl Acetate 5.4 Polysorbate 60 3.2  Kabapol 1.0 Sorbitol 3.1  incense a very small amount antiseptic a very small amount Purified water Remaining amount  system 100

Formulation example  4

Formulation example of the massage cream in the cosmetic containing the herbal extract of the present invention is as follows.

    ingredient Content (% by weight)  Herbal extract 0.5  glycerin 4.0  vaseline 3.5  Triethanol amine 0.5  Floating paraffin 24.5  Squalane 2.5  Beeswax 2.1  Tocopheryl Acetate 0.1 Polysorbate 60 2.4  Kabapol 1.0 Sorbitan sesquioleate 3.1  incense a very small amount antiseptic a very small amount Purified water Remaining amount  system 100

Formulation example  5

Formulation examples of the pack in the cosmetic containing the herbal extract obtained above are as follows.

ingredient Content (% by weight) Herbal extract 1.0  Ethyl alcohol 3.0 EDTA-2Na 0.02  Propylene glycol 5.1  glycerin 4.5  Kabapol 1.0  Polyoxide 0.1  antiseptic a very small amount  incense a very small amount  Purified water Remaining amount  system 100

As described above, the present invention additionally includes herbal extracts in the conventional cosmetics, promoting the proliferation of fibroblasts, keratinocyte proliferation and collagen synthesis, and improves the skin's moisturizing ability, which is very effective in preventing skin aging. In addition, since the irritation to the skin is very small, it is very useful as a cosmetic composition for preventing skin aging without a side effect on the skin and a method for producing the same.

In the above, the present invention has been illustrated and described with reference to specific preferred embodiments, but the present invention is not limited to the above-described embodiments and is not limited to the spirit of the present invention. Various changes and modifications can be made by those who have

Claims (4)

In a cosmetic composition comprising an antioxidant, a stabilizer, a solubilizer, a vitamin, a pigment, and a perfume, A cosmetic composition for preventing skin aging, comprising a herbal extract extracted with a blending ratio of 5 g of licorice, 10 g of golden leaf, 5 g of gold, 2 g of yellow lotus, and 2 g of mushrooms based on 500 ml of distilled water. The method of claim 1, The herbal extract is a cosmetic composition for preventing skin aging, characterized in that 0.0001-10.0% by weight relative to 100% by weight of the total cosmetic composition. The method according to claim 1 or 2, The cosmetic composition is any one selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays. Cosmetic composition for preventing skin aging, characterized in that having a formulation. In the method of manufacturing a cosmetic composition made of antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings, Licorice 5g, 10g thick, 5g golden, 2g rhubarb, 2g of mushrooms, finely crushed using a crusher, 500ml of distilled water is added to it, then decocted at 50 ℃ for 4 hours, filtered and cooled at room temperature for 2 days Method for producing a cosmetic composition for preventing skin aging, characterized in that it comprises a medicinal herb extract prepared by aging.