KR101558186B1 - Cosmetic composition containing Phyllanthus urinaria extrancts - Google Patents

Abstract

The present invention relates to a cosmetic composition having an anti-aging, antioxidant or wrinkle-reducing effect. More specifically, the present invention relates to a cosmetic composition having an anti-aging, antioxidant or wrinkle- And inhibiting the biosynthesis of cyclooxygenase-2 and inhibiting the biosynthesis of tumor necrosis factor-alpha, thereby having anti-aging, antioxidant or wrinkle-reducing effects.

Fox bead extract, anti-aging, antioxidant, wrinkle improvement, cosmetic composition

Description

[0001] Cosmetic composition containing Phyllanthus urinaria extrancts containing a fox bead extract [

The present invention relates to a cosmetic composition having an anti-aging, antioxidant or wrinkle-reducing effect. More specifically, the present invention relates to a cosmetic composition having an anti-aging, antioxidant or wrinkle- And inhibiting the biosynthesis of cyclooxygenase-2 and inhibiting the biosynthesis of tumor necrosis factor-alpha, thereby having anti-aging, antioxidant or wrinkle-reducing effects.

The human skin undergoes changes due to various internal and external factors as it gets older. In other words, internally, secretion of various hormones that regulate metabolism is reduced, the function of immune cells and the activity of cells are lowered, and the biosynthesis of immune proteins and biocompatible proteins necessary for the living body is reduced, and ozone layer destruction As the amount of ultraviolet rays reaching the surface of the sunlight increases and environmental pollution is further intensified, free radicals and active harmful oxygen are increased, thereby reducing the thickness of the skin, increasing the wrinkles and decreasing the elasticity Skin color is also gray, skin troubles occur frequently, and spots, freckles and black spots also increase.

As the aging progresses, the content and arrangement of collagen, elastin, hyaluronic acid and glycoprotein, which constitute the skin, are changed or decreased, and oxidative stress due to free radicals and active noxious oxygen is experienced. Cox-2 (cyclooxygenase), an enzyme that produces a proinflammatory cytokine, known to cause inflammation in most cells constituting the skin by aging or ultraviolet rays, And the biosynthesis of Matrix metalloproteinase (MMP), an enzyme that degrades skin tissue, is increased by these inflammatory factors, and nitric oxide (NO) production by iNOS (inducible nitric oxide synthase) Respectively. In other words, decrease of cellular activity due to natural endogenous aging and decrease of biosynthesis of substrate material by microinflammation, increase of stress due to various harmful environment, increase of active oxygen species by sunlight, As the degradation and denaturation are accelerated, the skin substrate is destroyed and thinned, and various symptoms of skin aging appear. Therefore, it is a reality that many researches have been conducted on active ingredients that can prevent and improve such aging phenomena.

Fox beads ( Phyllanthus urinaria ), also known as juvenile or pearl juice , mainly grows in fields and grasses. It is 15 to 40 cm high, reddish, with branches spreading, narrow wings on the ground, with leaves alternating and running in 2 rows, appear like a twig leaf. There are several triangular leaves shaped like inverted eggs at the bottom, the leaves are elliptical, the ends are round, and the edges are flat. The back of the leaf is white and the petiole is short. The flower is a single flower, blooming in July-August, and reddish brown. 6 hairs, 3 stamens, 1 pistil. Fruit is capillary (果), with a sagittal shape, without a bag, with a reddish brown color and a wrinkled side. It is similar to a fox bag, but has no bag of fruit, and is widely distributed in Korea, Japan, and tropical and subtropical regions. In addition, fox beads have therapeutic effects on enteritis, dysentery, infectious hepatitis, and nephritis, urinary tract infections, nematodes, soreness markers, Is known.

Korean Patent Laid-Open Publication No. 2004-59004 discloses a composition for skin whitening comprising a plant extract having an activity of inhibiting melanin formation, which comprises at least one member selected from the group consisting of pearl bean juice extract, .

Japanese Patent Laid-Open Publication No. Hei 8-12566 discloses an extract of Phyllanthus niruri L., Piper elongatum Vahl. & Piper ungustifolium ) and Western nettle ( Urtica urens ) in combination with at least one selected from the group consisting of at least one selected from the group consisting of at least one kind of tyrosinase activity inhibitor.

Accordingly, the inventors of the present invention have conducted research on anti-aging cosmetics that inhibit skin aging, and found that fox bead extract promotes collagen production in skin, inhibits MMP-1, protects DNA damage, and biosynthesis of cyclooxygenase- And suppresses the biosynthesis of tumor necrosis factor-alpha, thereby exhibiting an anti-aging, antioxidant or wrinkle-reducing effect, thus completing the present invention.

Accordingly, an object of the present invention is to provide a method for inhibiting MMP-1, protecting DNA damage, suppressing biosynthesis of cyclooxygenase-2, inhibiting biosynthesis of tumor necrosis factor- Anti-aging, antioxidant or wrinkle-improving effect.

In order to achieve the above object, the present invention provides an anti-aging cosmetic composition comprising a fox bead extract as an active ingredient.

In addition, the present invention provides an antioxidant cosmetic composition containing a fox bead extract as an active ingredient.

The present invention also provides a cosmetic composition for improving wrinkles containing a fox bead extract as an active ingredient.

The cosmetic composition according to the present invention promotes collagen production in skin by containing fox marbled extract as an active ingredient, inhibits MMP-1, protects DNA damage, suppresses the biosynthesis of cyclooxygenase-2, It was possible to provide a cosmetic composition having anti-aging, antioxidant or wrinkle-reducing effects by inhibiting the biosynthesis of necrotic factor -?.

Hereinafter, the present invention will be described in more detail.

The present invention relates to an anti-aging, antioxidant or wrinkle-improving cosmetic composition containing a fox bead extract as an active ingredient.

The fox bead extract used in the present invention can be prepared by extracting fox beads with water or an organic solvent by a method well known in the art. The organic solvent used in the present invention may be at least one selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate and chloroform, or a mixed solvent of these organic solvents and water may be used. Preferably, 80% ethanol is used.

The cosmetic composition according to the present invention may contain 0.001 to 10% by weight, preferably 0.01 to 5% by weight, of the fox bean extract, based on the total weight of the composition. If the content of the fox bean extract is less than 0.001% by weight, the effect of anti-aging, antioxidation and wrinkle improvement by the extract can not be obtained, and if it exceeds 10% by weight, the increase of the effect is not so significant.

The cosmetic composition according to the present invention may be formulated containing a cosmetically or dermatologically acceptable medium or base. It may be any formulation suitable for topical application, for example, as a solution, a gel, a solid, a paste anhydrous product, an emulsion obtained by dispersing an oil phase in water, a suspension, a microemulsion, a microcapsule, In the form of a non-ionic follicle dispersing agent, or in the form of creams, skins, lotions, powders, ointments, sprays or conical sticks. These compositions may be prepared according to conventional methods in the art. The composition according to the invention may also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

The cosmetic composition according to the present invention can be used as a cosmetic composition in the form of a cosmetic composition containing a lipid, an organic solvent, a solubilizer, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, Such as fillers, emulsifiers, emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, blockers, moisturizers, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredient commonly used in cosmetics May contain adjuvants commonly used in the field of cosmetics or dermatology. Such adjuvants are introduced in amounts commonly used in the cosmetics or dermatological fields.

The cosmetic composition according to the present invention is not particularly limited in its formulation. Examples of the cosmetic composition according to the present invention include softening lotion, convergent lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, eye essence, cleansing cream, Cleansing water, a pack, a powder, a body lotion, a body cream, a body oil, and a body essence.

Hereinafter, the constitution and effects of the present invention will be described in more detail with reference to examples and test examples. However, these examples and examples are provided for illustrative purposes only in order to facilitate understanding of the present invention, and the scope and scope of the present invention are not limited by the following examples.

[Example 1] Preparation of fox bead extract

To 0.5 kg of dried fox beads, 2 L of a 70% ethanol aqueous solution of 4 times the weight ratio was added, and the mixture was refluxed three times and then immersed at 25 DEG C for 3 days. Thereafter, the filtrate was separated by filtration and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain a fox bead extract.

[Test Example 1] Antioxidant activity of fox bead extract

The antioxidant effect was investigated by comparing the ability to remove reactive oxygen species (ROS) generated in cells by ultraviolet (UV) irradiation. The results are shown in FIG. As a positive control group, trolox, which is generally used for comparing antioxidative effects, was used, and a group not treated with the test substance was used as a control group.

1, the fox bead extract of Example 1 according to the present invention significantly inhibited the active oxygen generated by UV in the human HaCaT keratinocytes monolayer culture system (human HaCaT keratinocytes monolayer culture system) . This was an excellent activity similar to that of trolox used as an activity index of antioxidants. Through this, the extract of fox marbles according to Example 1 of the present invention significantly eradicated active oxygen which causes skin aging, Production and elasticity of the water-soluble polymer.

[Test Example 2] Inhibitory activity against DNA oxidative damage by single cell gel electrophoresis (SCGE, Comet assay)

Human normal fibroblasts were cultured for 24 hrs. Then, the fox bead extract and vitamin E of Example 1 were treated for each concentration, treated with a positive control group of H ₂ O ₂ (final concentration: 10 -3 M) after 12 hours, and then subjected to electrophoresis The result of the staining was observed with a fluorescence microscope. At this time, the length (um) of the tail as a result of DNA cleavage in each of 25 cells was analyzed using an image analyzer, KOMET 3.1 (Kinetic Imaging, England) Respectively.

Inhibitory effect of fox bead extract, vitamin E on DNA oxidative damage Test substance Tail length (탆) Inhibition rate (%) H ₂ O ₂ 110.6 - H ₂ O ₂ + Example 1 (10 ppm) 65.8 24.4 H ₂ O ₂ + Example 1 (50 ppm) 55.7 33.2 H ₂ O ₂ + Example 1 (100 ppm) 42.1 44.9 H ₂ O ₂ + vitamin E 10 ppm 60.6 38.9

In the results shown in Table 1, the fox bead extract of Example 1 by cell gel electrophoresis showed significant oxidative DNA damage inhibitory activity, and thus the fox bead extract according to the present invention inhibited the hydroxyl radical (.OH) , Which is a DNA oxidative damage inhibitor that suppresses DNA single strand break caused by DNA damage.

[Test Example 3] I-type procollagen assay (Type I Procollagen assay)

Human fibroblasts were cultured in a 12-well plate incubator at a concentration of 10 ⁵ and then replaced with a medium containing 1 ppm and 10 ppm of the fox bead extract of Example 1. On the third day of culture, the cells were harvested and the amount of type I procollagen produced by the ELISA method was quantified. The results are shown in FIG. The results are shown in Table 1, which is a comparison with the control value of the control group not containing the fox bead extract of Example 1 as 100, and TGF-b was used as a positive control.

As shown in the results of FIG. 2, in the normal human fibroblast monolayer culture system, the fox bee extract of Example 1 showed a markedly enhanced effect on the formation of type I procollagen compared with the control group . That is, the extract of fox marbles of Example 1 suppressed the reduction of collagen production caused by aging of human skin, and showed an effect of improving wrinkles.

[Test Example 4] MMP-1 expression inhibition assay

Human fibroblasts were cultured in a 12-well plate incubator at a concentration of 10 ^5, irradiated with ultraviolet ray B at 40 mJ / cm 2, and then replaced with a medium containing 1 ppm and 10 ppm of fox bead extract of Example 1. On the second day of culture, the cells were harvested and the amount of MMP-1 (matrix metalloproteinase I) produced by the ELISA method was quantified. The results are shown in FIG. The above results were calculated by comparing the values measured with the ultraviolet control group containing no test substance as 100, and TGF-b was used as the positive control group.

As shown in the results of FIG. 3, in the human normal fibroblast monolayer culture system, the fox bead extract of Example 1 according to the present invention significantly inhibited the expression of MMP-1 induced by UVB 40 mJ / cm 2 . Thus, the fox marbling extract of Example 1 according to the present invention inhibits the biosynthesis of MMP-1, a skin tissue degrading enzyme caused by internal aging or external environmental factors, thereby suppressing skin aging and improving wrinkles Respectively.

[Test Example 5] Effect of inhibiting the biosynthesis of cyclooxygenase-2 (COX-2)

Human fibroblasts were cultured in a 12-well plate incubator at a concentration of 10 ^5, irradiated with UVA at 15 J / cm 2, and then replaced with media containing 0.1 pp, 1 ppm and 10 ppm of fox bead extracts of Example 1, respectively. On the second day of culture, cells were harvested and the amount of cyclooxygenase-2 (COX-2) produced using the Westren blot method was quantitated. The results are shown in Table 2 below. The results are shown in Table 2 below. The results are shown in Table 2 below.

Test substance (concentration: ppm) COX-2 biosynthesis (%) Example 1 10 15 One 28 0.1 73 Control group 100

From the results shown in the above Table 2, the fox bean extract of Example 1 decreased the biosynthesis of cyclooxygenase-2 by ultraviolet light in a concentration-dependent manner, and the skin tissue degradation by prostaglandin E2 (PGE2) . ≪ / RTI >

[Test Example 6] Inhibitory effect of ultraviolet light on the biosynthesis of tumor necrosis factor alpha (TNF-α)

Human keratinocytes were cultured in a 12-well plate incubator at a concentration of 10 ⁵ , irradiated with ultraviolet ray B at 30 mJ / cm 2, and then replaced with media containing 0.1 pp, 1 ppm and 10 ppm of fox bead extracts of Example 1, respectively. Cells were harvested 6-24 hours after culture and the amount of tumor necrosis factor alpha (TNF-a) produced was quantitated using ELISA (Pharmingen 555212) method, The results are shown in Table 3 below.

Test substance (concentration: ppm) TNF-α biosynthesis (%) Example 1 10 16 One 38 0.1 72 Control group 100

From the results shown in the above Table 3, it can be seen that the fox bead extract of Example 1 according to the present invention can be produced by the biosynthesis of tumor necrosis factor alpha by reducing the biosynthesis of tumor necrosis factor alpha (TNF-a) It was confirmed that skin aging can be prevented in advance.

[Example 2 and Comparative Example 1]

The nutritional creams of Example 2 and Comparative Example 1 were prepared according to the compositions shown in Table 4 below in the usual manner (unit:% by weight)

ingredient Example 2 Comparative Example 1 The fox bead extract of Example 1 0.1 - Wax 10 10 Polysorbate 60 1.5 1.5 Piggy 60 hardened castor oil 2 2 Sorbitan sesquioleate 0.5 0.5 Liquid paraffin 10 10 Squalane 5 5 Caprylic / capric triglyceride
(Estasan: Uniqema (manufacturer)) 5 5 glycerin 5 5 Butylene glycol 3 3 Propylene glycol 3 3 Triethanolamine 0.2 0.2 Purified water Balance Balance

[Test Example 7] Effect of improving skin wrinkles on human skin

Forty patients who had facial wrinkles of 30-50 years old were used in Example 2 for the left side of the subject and Comparative Example 1 for 3 months for the right side. The skin condition of both sides of the face before the use of the cream was measured, and then the same site was re-measured after 3 months of use of the cream. The skin wrinkles were measured with a Visiometer system (C + K company) by removing the wrinkles of the eye tail region with a replica in a constant temperature and humidity chamber at 24 ° C and a relative humidity of 40%. The amount of change in the skin wrinkles was calculated according to the following formula (1).

In the equation (1), "Tdi" is the measured site value at D90 and "Tdo" is the measured site value at D0.

As a result of calculation according to Equation (1), skin wrinkles at the site using Comparative Example 1 showed a decrease of 6.5 +/- 4% (mean 占 standard deviation), while skin wrinkles at the site using Example 2 were 39 占 11% Of the total wrinkles.

[Test Example 8] Effect of improving skin elasticity in human body

The nutritional cream of Example 2 and Comparative Example 1 was applied to the face for 2 weeks each for 12 weeks in each group. The skin elasticity meter (Cutometer SEM 575, C + K Electronic Co., Germany). The results are shown in Table 5 below. The results in Table 5 indicate the viscoelasticity of the skin elasticity measuring device.

Test substance Skin elasticity effect Example 2 0.46 + 0.12 Comparative Example 1 0.29 ± 0.09

As shown in the results of Table 5, when the nutritional cream of Example 2 containing the fox bean extract of Example 1 was used, the skin elasticity was improved by about 150%.

As described above, it was confirmed that the use of Example 2 containing the fox bead extract according to the present invention improves skin wrinkles and skin elasticity, thereby contributing to suppression and delay of skin aging.

[Formulation Example 1] Softening lotion (skin lotion)

Compounding ingredient Content (% by weight) Purified water Balance The fox bead extract of Example 1 0.1 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 Phage-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1

[Formulation Example 2] Nutritional lotion (Milk lotion)

Compounding ingredient Content (% by weight) Purified water Balance The fox bead extract of Example 1 0.1 Wax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Montana 202 (Manufacturer: Seppic) 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Triethanolamine 0.2

[Formulation Example 3] Massage cream

Compounding ingredient Content (% by weight) Purified water Balance The fox bead extract of Example 1 0.1 Wax 10.0 Polysorbate 60 1.5 Piggy 60 hardened castor oil 2.0 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Montana 202 (Manufacturer: Seppic) 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2

[Formulation Example 4] Pack

Compounding ingredient Content (% by weight) Purified water Balance The fox bead extract of Example 1 0.1 Polyvinyl alcohol 13.0 Sodium carboxymethylcellulose 0.2 glycerin 5.0 Allantoin 0.1 ethanol 6.0 Phage-12 nonylphenyl ether 0.3 Polysorbate 60 0.3

FIG. 1 is a graph showing the active oxygen elimination activity of the fox bead extract of Example 1. FIG.

FIG. 2 is a graph showing the amount of collagen production of the fox bead extract of Example 1. FIG.

FIG. 3 is a graph showing the effect of the extract of fox marbles of Example 1 on the inhibition of MMP-1 biosynthesis. FIG.

Claims (5)

An anti-aging cosmetic composition comprising 70% ethanol aqueous solution of fox beads as an active ingredient. An antioxidant cosmetic composition comprising 70% ethanol aqueous solution of fox beads as an active ingredient. A cosmetic composition for improving skin wrinkles containing 70% ethanol aqueous solution of fox beads as an active ingredient. 4. The cosmetic composition according to any one of claims 1 to 3, wherein the cosmetic composition comprises 0.001 to 10% by weight of a 70% ethanol aqueous solution extract of the fox beads based on the total weight of the composition. The cosmetic composition according to any one of claims 1 to 3, wherein the cosmetic composition is at least one selected from the group consisting of softening agents, convergent lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, Wherein the cosmetic composition has one of the formulations selected from the group consisting of a powder, a powder, a body lotion, a body cream, a body oil, and a body essence.

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