KR20080108065A - Manufacturing method of cometic compositions containing extract of paeonia suffruticosa andrew - Google Patents

Abstract

A method for preparing a cosmetic composition is provided to relieve skin wrinkle, to improve the elasticity of the skin and to enhance the antiaging effect of the skin. A method for preparing a cosmetic composition comprises the step of using an extract of Paeonia suffruticosa Andrew, wherein the extract is prepared by using a mixture extraction solvent comprising water, an alcohol-based solvent and a glycol-based solvent in a ratio of 2:1:1 by weight. Preferably the alcohol-based solvent is at least one selected from the group consisting of methanol, ethanol, propanol and butanol; and the glycol-based solvent is at least one selected from the group consisting of ethylene glycol, propylene glycol and butylene glycol. The extract contains a polyphosphate of a concentration of 10.0-17.4 ng/mL.

Description

MANUFACTURING METHOD OF COMETIC COMPOSITIONS CONTAINING EXTRACT OF PAEONIA SUFFRUTICOSA ANDREW}

The present invention relates to a method for preparing a cosmetic composition containing Paeonia suffruticosa Andrew extract, and more particularly, in extracting the active ingredient from the neck skin, by extracting to contain a high concentration of the polymer, it has an anti-aging effect It relates to an excellent cosmetic composition. The bark extract is extracted from a mixed extractant including water, alcohol and glycol. According to the present invention, a high concentration of phosphorus is contained in the extract of the bark, and the antioxidant effect of the phosphorus, cell growth promotion, collagen synthesis increase, and collagenase inhibitory effect are effectively utilized, and thus excellent skin anti-aging effect Has

The present invention relates to a method for producing a cosmetic composition containing the extract of the bark, more specifically in extracting the active ingredient from the bark skin, by extracting to contain a high concentration of the polymer, the antioxidant effect of the polymer, promoting cell growth The present invention relates to a method for producing a cosmetic composition having an excellent anti-aging effect by effectively using collagen synthesis and inhibiting collagenase.

Aging is a process of gradual deterioration of the body's structure and function, a rapid increase in susceptibility to disease and death, and a physiological change in the body, resulting in a significant deterioration of the entire body. . Skin aging is the most important thing we can feel. It is recognized as important as diabetes, heart disease, arteriosclerosis, dementia cancer, etc. Although there are individual differences, skin aging usually begins around the early 20s and is older. It appears to increase as it grows.

Skin aging is influenced by various factors, genetic and environmental, which can be divided into two types. One is intrinsic aging, which is a natural aging phenomenon that occurs over time according to genetic information, and the other is called extaging aging, also known as photoaging. It refers to the aging phenomenon caused by the ultraviolet rays present. Endogenous aging is mainly observed in skin that is not exposed to sunlight, characterized by wrinkles and reduced skin elasticity, and photoaging occurs in areas exposed to sunlight, deep wrinkles, rough skin, pigmentation, It is characterized by a decrease in skin tone and skin elasticity.

In the dermis of the skin, collagen and elastin form a network structure to maintain the elasticity of the skin. However, when these network structures are broken, it is known that the elasticity of the skin is reduced, the elasticity of the skin is reduced, and the wrinkles of the skin are formed as the dermis-epidermal boundary becomes flat. The histological characteristics of photo-aged skin show that the amount of collagen and elastin protein present in the dermis is significantly reduced and damaged compared to endogenous aging, resulting in the appearance of deep wrinkles and decreased elasticity. Therefore, when exposed to ultraviolet rays for a long time, a thick wrinkle is formed, one of the causes is the increase of matrix metalloproteinase (MMP), a matrix proteinase. When UV rays are irradiated to human skin, these enzymes are increased through several signaling pathways, which break down collagen and elastin in the dermis and promote wrinkle development.

Another factor is the increase in reactive oxygen species (ROS). Free radicals are by-products of the body's metabolic processes and are called free radicals called harmful oxygen or free radicals. Free radicals are materials that have unpaired electrons, which are very reactive and have a very short lifespan. Representatives of active oxygen are unstable in the form of radicals called superoxides (O2-), which are more oxidative than oxygen. It is a large species. Hydrogen peroxide (H2O2), hydroxyl group (OH-), alkyl peroxide, and halogen oxide, which have high oxidizing power, are all classified as active oxygen species. These reactive oxygen species are known as substances that attack various components of the body, such as DNA, proteins, sugars, lipids, and so on, destroying their structure and function, causing various diseases of the body and causing aging. However, the body protects the body from free radicals by synthesizing the superoxide dismutase (SOD), an antioxidant enzyme, to automatically remove free radicals when they occur. However, as we age, not only the protection against free radicals decreases, but also the free radicals increase due to stress, external contaminants and ultraviolet rays, and antioxidant enzymes decrease. The increased free radicals destroy collagen and elastin in the dermis and increase the production of matrix degrading enzymes, thereby promoting skin aging and wrinkles.

On the other hand, the Republic of Korea Patent No. 0592513 is suggested that the phosphor has excellent antioxidant action. In addition, the Republic of Korea Patent Publication No. 2003-0064059 proposes a cosmetic composition containing the extract of the bark as an ingredient for the antioxidant and whitening effect.

However, the cosmetic composition according to the prior art, in extracting the active ingredient from the bark skin, does not take an extraction method that may contain a large amount of the polymer, there is a problem that the effect of the content of the bark skin extract is insignificant.

As a result of repeated studies for preventing skin aging, the present inventors have found that the in-polymer contained in the extract of the bark extract is effective in preventing skin aging by improving skin wrinkles and improving elasticity. At this time, in the preparation of the extract of the bark, when using a mixture of three different extraction solvents as the extraction solvent, it was found that a large amount of the polymer is completed to complete the present invention. In addition, it was found that the inpolymer has an antioxidant effect as well as promoting cell growth, promoting collagen synthesis, and inhibiting collagenase, thereby having an excellent effect on preventing skin aging.

In order to solve the above problems, the preparation method of the cosmetic composition containing the extract of the bark of the present invention comprises the following means.

According to an embodiment of the present invention, the method for preparing a cosmetic composition containing the extract of bark is in the cosmetic composition containing the extract of the bark, the extract of the bark is extracted from a mixed extract solvent including water, alcohol and glycol, Alcohol is at least one selected from the group consisting of methanol, ethanol, propanol and butanol, the glycol is at least one selected from ethylene glycol, propylene glycol and butylene glycol, the water, alcohol and glycol is 2: It is mixed in a weight ratio of 1: 1 so as to obtain an inpolymer from the bark skin, the extract of the wood skin is characterized in that it contains a 10.0 ~ 17.4 ng / ml concentration of the polymer.

In addition, the extract of the neck bark is characterized in that obtained by adding the mixed extract solvent in an amount of 10 to 30 times the weight of the neck bark, extracted for 5 to 20 hours at a temperature of 50 ~ 100 ℃.

The cosmetic composition prepared by the present invention, but the extract of the bark skin as an active ingredient, the extract is excellent in the anti-aging effect of the skin is contained in a large amount of a polymer in a high concentration. Specifically, the anti-oxidant effect, the cell growth promotion, collagen synthesis promotion and collagenase inhibitory effect and the like possessed by the phosphor is effectively utilized to have an excellent skin anti-aging effect by improving the skin wrinkles, improve the moisturizing power and enhance the elasticity. In addition, the extract of the bark skin is excellent skin safety, there is no skin irritation.

Hereinafter, the present invention will be described in detail.

Peony ( Paeonia suffruticosa Andrew) used in the present invention is the peony or peony of Peony, which is a deciduous broad-leaved shrub of cold winter, native to western China, and is believed to be a naturalized plant introduced in China due to the records of Queen Seondeok in the Silla period. It is becoming. The leaves are two right upper lobes, divided into three parts, the lobules are ovate or lanceolate, often divided into 3-5, the surface is hairless, the back side has fine hairs, and is generally white. Fruits are intact, dense with short hairs, ripen in July-September, burst out from the inner end, and the seeds are round and black. Flower is bisexual, blooms in May, has about 10 petals, over 15cm in diameter, and one large and small flower blooms at the end of new branches. The color of flowers is usually purple, but there are various varieties such as dark red, pink, yellow, white, and purple. The flower becomes a pocket and surrounds the room. Calyx leaves are 5, petals are 8 or more, they are not the same size and shape, obovate, irregular kerchief on the edge. There are many surgeries and pistils are 2-6, with hairs. The stem reaches 2m in height, the branches are thick, the stem is 15cm in diameter, and there is no hair. The roots are thick and rare, but the roots are small, and the bark of the roots is called Peony, in Chinese medicine, it is anti-inflammatory and analgesic. It is known to be used for the treatment of.

The cosmetic composition according to the present invention contains the above extract of the bark as an active ingredient. In general, the bark skin has excellent physiological activities such as polyphosphate, paeonol, paeonoside, paeonolide, paeoniflorin, phytosterol, etc. It is known that it contains a lot of active ingredients.

The bark extract is extracted from blood such as stems or roots of the bark, wherein the bark extract is extracted through an extraction method that may contain a large amount of the polymer according to the present invention. Specifically obtained by the extraction method described below.

Bark wood is dried in a cool place and then physically comminuted, and then extracted by adding an extraction solvent to the comminuted product. At this time, the extraction solvent is used to contain three or more different extraction solvents, specifically, a mixed extraction solvent containing at least water, alcohol and glycol-based so as to contain a high concentration of the polymer. According to the present invention, in the preparation of extract of the bark, when using three or more different extraction solvents as described above it can be prepared an extract containing a high concentration of the polymer. In this case, the mixed extraction solvent is preferably mixed with water, alcohol-based and glycol-based in a weight ratio of 1: 0.25 ~ 1.0: 0.25 ~ 1.0.

In addition, the water constituting the mixed extraction solvent is preferably distilled water, the alcohol-based is preferably used at least one selected from a water of 1 to 4 carbon atoms or anhydrous alcohol. Specifically, the alcohol system is one or more selected from the group consisting of methanol, ethanol, propanol and butanol. In addition, the glycol system is preferably used at least one selected from ethylene glycol, propylene glycol and butylene glycol.

At this time, in adding the mixed extract solvent to the pulverized wood skin, it is preferable to add the mixed extract solvent in an amount of 10 to 30 times the dry weight of the wood skin. That is, the ratio of the mixed extraction solvent and the pulverized product is preferably in the weight ratio of 1:10 to 1:30, and more preferably in the weight ratio of 1:20. And after adding the mixed extraction solvent to the pulverulent crushed wood as described above is extracted for 5 to 20 hours at a temperature of 50 ℃ ~ 100 ℃.

As for the extract of neck peel used in the present invention, the extract using the above-mentioned mixed extracting solvent is used as it is, or after the purification process, for example, membrane separation of various membranes such as ultrafiltration membranes having a constant molecular weight cut-off value, and various chromatography ( It may include those obtained by further carrying out purification processes such as separation by size, charge, hydrophobicity or affinity). In addition, the extract may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze-drying (or spray drying), and then contained in the composition as it is, or diluted in a solvent such as ethanol.

As described above, according to the extraction method through a mixed extraction solvent, it is possible to prepare a dermal extract containing 10.0 ng or more of the inpolymer per ml of extract. Specifically, through the extraction method as described above it can be obtained extract of the bark containing the polymer of the concentration of 10.0 ~ 20.0 ng / ml. As such, the extract of the bark dermis has an excellent anti-aging effect when the extract contains 10.0 ng / ml or more of an inpolymer.

The cosmetic composition according to the present invention is included in the present invention as long as it contains the extract of the bark obtained through the above extraction method. And the cosmetic composition according to the present invention may further contain at least one active ingredient exhibiting the same or similar function in addition to the extract of the neck bark.

Preferably, the extract is preferably contained in an amount of 0.001% to 30.0% by weight based on the total weight of the cosmetic composition. More preferably contained 0.1 to 10.0% by weight. At this time, when the content of the extract is less than 0.001% by weight, it is difficult to expect the effect according to its content, when it exceeds 30.0% by weight, the other components are relatively small and may negatively affect the formulation of the composition, As the content increases, the increase in the apparent effect may be insignificant.

The cosmetic composition according to the present invention is useful for preventing and improving skin aging. Specifically, the bark extract is contained, but the macromolecular extract contains a large amount of a polymer, the antioxidant effect, the cell growth promotion, collagen synthesis promotion and collagenase inhibitory effect is increased. Accordingly, skin wrinkle improvement, moisture retention and elasticity are enhanced to have an excellent skin anti-aging effect.

The cosmetic composition according to the present invention includes components commonly used in cosmetic compositions in addition to the extract of neck peel, and may include, for example, conventional inclusion agents such as stabilizers, solubilizers, vitamins, pigments and flavors, and carriers.

In addition, the cosmetic composition according to the present invention may be prepared in any formulation commonly prepared in the art, for example, containing solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactants. It may be formulated by cleansing, etc., but is not limited thereto.

At this time, when the formulation of the cosmetic composition according to the present invention is a paste, cream or gel as a carrier component, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc And one or more selected from the group consisting of zinc oxide and the like.

In addition, when the formulation of the cosmetic composition according to the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzo. One or more selected from the group consisting of eightes, propylene glycol, 1,3-butylene glycol, oils, glycerol aliphatic esters, polyethylene glycols, fatty acid esters of sorbitan, and the like can be used.

In addition, when the formulation of the cosmetic composition according to the present invention is a powder or spray, one or more selected from the group consisting of lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, etc. may be used as a carrier component. , In the case of sprays in particular, may further comprise propellants such as chlorofluorohydrocarbons, propane / butane or dimethyl ether.

In addition, when the formulation of the cosmetic composition according to the present invention is a suspension, a liquid diluent such as water, ethanol, or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbent as carrier components Suspending agents such as non-elastic esters, microcrystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

In addition, in the case where the formulation of the cosmetic composition according to the present invention is a surfactant-containing cleansing agent, as a carrier component, an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, One or more selected from the group consisting of sarcosinates, fatty acid amide ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, ethoxylated glycerol fatty acid esters, and the like can be used. .

The cosmetic composition according to the present invention has a formulation specifically selected from the group consisting of softening cream, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder. Can be.

Hereinafter, the present invention will be described in more detail with reference to Examples. The following examples are provided to explain the present invention in more detail, and it will be apparent to those skilled in the art that the technical scope of the present invention is not limited by these examples. will be.

[Production Example 1]

<Preparation of Bark Extracts>

First, commercially available bark skins were dried in a cool shade. Thereafter, using a grinder, finely pulverized dry wood skin, 200g of the pulverized product was put in a reflux extractor, 20 times distilled water of the weight of the ground wood skin was added thereto, and heated at 75 ° C. for 10 hours to extract hot water. The extract was filtered with a 400 mesh filter cloth, and the filtrate was collected using a freeze dryer to obtain a dry extract having a dry weight of 28.6 g. The dry extract was dissolved in 70% ethanol and used as follows.

[Production Examples 2 to 17]

<Preparation of Bark Extracts>

To prepare the extract of the bark through the same extraction method as in Preparation Example 1, as shown in the following [Table 1] was prepared by varying the extraction solvent according to each preparation example.

Production Example Extraction solvent Dry weight (g) One water 28.6 2 Methanol 27.1 3 ethanol 29.4 4 Propanol 25.6 5 Butanol 27.6 6 Acetone 22.1 7 Ethyl acetate 23.4 8 chloroform 8.7 9 glycerin 26.4 10 Butylene glycol 28.6 11 Propylene glycol 26.7 12 Dichloromethane 9.1 13 Hexane 16.4 14 benzene 14.7 15 Water: Methanol: Propylene Glycol (2: 1: 1) 28.3 16 Water: Ethanol: Propylene Glycol (2: 1: 1) 27.5 17 Water: Ethanol: Butylene Glycol (2: 1: 1) 28.1

Experimental Example 1

<Quantitative Determination of Inpolymers of Bark Extracts>

In order to measure the inpolymer content of the extracts of neck bark prepared in Preparation Examples 1 to 17, 100 μl (0.1 mg / ml) of each extract obtained in Preparation Example was placed in a test tube, and 0.6 ml of 0.5N HCl was added. After the addition, the mixture was placed in a constant temperature water bath at 37 ° C. for 15 minutes, and immediately cooled. In addition, 1.4 ml of molybdenum-ascorbate reagent (0.42% ammonium molybdate in 1N H ₂ SO ₄ : 10% ascorbic acid in dH ₂ 0, 6: 1, v / v) was added and incubated at 45 ° C for 20 minutes. (incubation). Next, the absorbance was measured at 820 nm using a spectrophotometer (Molecular Devices, USA), and then the inpolymer content (ng / ml) was evaluated based on this. The results are shown in the following [Table 2].

       <Quantitative results of phosphate polymer> Production Example Inpolymer Content (ng / ml) One 7.5 2 7.1 3 7.6 4 7.9 5 7.4 6 4.4 7 4.4 8 1.7 9 6.1 10 7.2 11 7.4 12 1.3 13 1.2 14 2.0 15 11.6 16 17.4 17 11.4

As shown in [Table 2], from the quantitative measurement result of the polymer, it was found that when the extraction was carried out using a mixed solvent as in Preparation Examples 15 to 17, it was found that the most polymer contained. In particular, when using an extraction solvent in which water, ethanol and propylene glycol were mixed in an appropriate ratio (2: 1: 1) as in Preparation Example 16, it was found that the phosphorus content was the highest.

Experimental Example 2

<Measurement of cell growth promoting effect>

In order to determine the effect of promoting the growth of cells with respect to the extract of the neck bark prepared in Preparation Examples 1 to 17 were performed as follows.

Human fibroblasts were aliquoted into 5 x 10 ³ cells in each well of a 96-well plate and incubated for 24 hours in medium containing 10% fetal bovine serum. Subsequently, after changing to a medium without fetal calf serum, the dermal extracts prepared in Preparation Examples 1 to 17 were treated with 0.005%, 0.05%, and 0.5% for each concentration of cells, and cultured for 48 hours. After 48 hours, 100 μl of culture medium MTT (methylthiazolydiphenyl-tetrazolium bromide, sigma) (5mg / ml) was added to the cells, incubated for 4 hours, the medium was removed, and 100μl of DMSO (dimethyl sulfoxide) was added to each well. After the addition, the absorbance was measured at 570 nm with an ELISA Reader (Molecular Devices, USA). At this time, the control group was used as not treated with the extract of the bark, cell proliferation capacity (%) was obtained through comparison with the control group. The results are shown in the following [Table 3].

             <Result of cell growth promoting effect> Production Example Cell proliferation (%) Treatment concentration (0.005%) Treatment concentration (0.05%) Treatment concentration (0.5%) Control 100.0 100.0 100.0 One 107.4 157.8 244.0 2 109.4 159.7 240.6 3 107.1 157.6 240.4 4 109.8 157.2 241.5 5 108.9 158.4 240.4 6 101.7 120.7 147.5 7 108.5 117.5 130.4 8 100.8 115.4 129.8 9 105.6 140.8 198.7 10 108.5 140.6 200.8 11 109.1 150.6 210.5 12 104.3 123.1 140.5 13 102.1 126.7 131.1 14 101.4 110.8 123.7 15 118.4 171.4 297.6 16 120.9 199.4 324.1 17 119.5 170.0 297.0

As shown in the [Table 3], it was found that the extract of the neck bark shows excellent cell proliferation ability compared to the control. In addition, it was found that the cell proliferation effect was excellent depending on the concentration of the extract of neck bark, in particular, it was found that the highest cell proliferation effect was obtained in Preparation Example 16, which has the highest inpolymer content.

Experimental Example 3

<Determination of Antioxidant Effect of Bark Extracts>

In order to investigate the antioxidant effect of the extract, the antioxidant effect was measured by thiobutylate acetic acid (TBA) test method using lipid peroxidation reaction system by Fenton reaction. Dodecylmethyl in which 0.05 g of the bark extract of Preparation Examples 1 to 17 was dissolved in 5 ml of 25 mM Tris buffer solution / 0.75 mM potassium buffer solution containing 10 µl of ethyl linoleate, 0.2% dodecyl sulfate, 10 µmol of ferric chloride, and 2 µmol of hydrogen peroxide. 0.1 ml of sulfate was added, followed by incubation at 55 ° C. for 16 hours, and then 50 µl of 4% butylhydroxytoluene was added and the test was stopped. Then, 0.3 ml of the solution was placed in a test tube, 0.05 ml of hydrochloric acid (3.0 ml) and butanol (4.0 ml) were added thereto, and the absorbance was measured at 535 nm. At this time, as a control group was not treated with the extract of the bark, the antioxidant effect (%) was calculated based on this. The results are shown in the following [Table 4].

      <Antioxidant effect measurement result> Production Example Antioxidative effect (%) One 39.4 2 37.2 3 39.9 4 41.8 5 38.5 6 26.7 7 26.4 8 8.2 9 30.1 10 37.5 11 38.6 12 4.1 13 3.6 14 8.5 15 73.0 16 89.4 17 72.2

From the results of the above [Table 4], it was found that the extract of the bark has an antioxidant effect, and particularly, the extract of the bark of the extract according to Preparation Example 16 containing a large amount of the polymer showed the best antioxidant effect.

Experimental Example 4

<Determination of Collagen Synthesis Effect of Bark Extracts>

Collagen synthesis effect was used to crystal violet (crystal violet) method. This method is an experiment to determine the ability of protein synthesis, crystal violet dye solution is a reagent that binds to the protein, utilizing the characteristics of the collagen production was measured as follows.

Human fibroblasts were aliquoted into 5 x 10 ³ cells in each well of a 96-well plate and incubated for 24 hours in medium containing 10% fetal bovine serum. Thereafter, after changing to a medium without fetal calf serum, 0.01% of the bark extract prepared in Preparation Examples 1 to 17 was treated with cells and cultured for 48 hours. After 48 hours, the culture was removed, washed once with PBS, and then dried at 37 ° C. for 30 minutes. Then, crystal violet reagent was added and stained at room temperature for 5 minutes, and the dye solution was washed well with distilled water. The combined dye was dissolved by adding 0.2 M phosphoric acid and ethanol, and then 5 minutes later with ELISA Reader (Molecular Devices, USA). Absorbance was measured at 590 nm. At this time, the control group was used as not treated with the bark extract, collagen production capacity (%) was obtained through comparison with the control group. The results are shown in the following [Table 5].

   <Result of Collagen Synthesis Effect> Production Example Collagen Production Capacity (%) Control 100.0 One 139.4 2 137.1 3 140.5 4 148.3 5 138.6 6 124.3 7 124.6 8 108.4 9 131.3 10 137.9 11 138.8 12 106.5 13 106.1 14 109.6 15 181.6 16 204.9 17 180.8

As shown in [Table 5], the extract of neck bark according to each preparation showed the collagen synthesis promoting effect, in particular, the extract of neck bark of Preparation Example 16 containing a large amount of the polymer showed the best collagen synthesis ability. Showed.

Experimental Example 5

<Measurement of collagenase inhibitory effect>

In order to investigate the inhibitory effect of collagenase activity of the bark extract, 0.1 mg collagenase (sigma, C5138) was dissolved in 1 ml of 0.1M tris buffer (pH 7.1), and then collagen (sigma, C9879) 0.5 After dissolving the mg in 1 ml of 0.1M tris buffer (pH 7.1), 0.5 ml of the bark extract prepared in Preparation Examples 1 to 17 was added to the test tube, 0.1 ml, and then collagenase 0.05 ml, and collagen. 0.4 ml was added, followed by reaction for 30 minutes in a 37 DEG C constant temperature water bath. Next, 1 ml of 25 mM citric acid solution was added to terminate the reaction. Then, 5 ml of ethyl acetate was injected into the test tube where the reaction was completed, and centrifuged at 3000 rpm for 5 minutes, and the absorbance of the ethyl acetate layer was measured at 320 nm. The control group was used not to process anything, the results are shown in Table 6 below.

    <Result of measuring collagenase inhibitory effect> Production Example  Collagenase activity inhibition rate (%) Control 0.0 One 65.7 2 61.4 3 66.8 4 67.4 5 65.1 6 21.2 7 21.3 8 11.8 9 53.7 10 61.8 11 65.1 12 9.8 13 9.4 14 16.8 15 83.1 16 96.7 17 82.0

As shown in the above [Table 6], it was found that the extract of the bark dermis was excellent in collagenase activity inhibition rate, in particular, the collagenase inhibition ability was the most effective in Preparation Example 16 containing the most polymer.

[Examples 1 and 2]

To prepare a cosmetic composition of the nutrition cream formulation with the ingredients and contents shown in the following [Table 7]. At this time, the bark extract of Preparation Example 13 was contained 5.0% by weight (Example 1), and the bark extract of Preparation Example 16 in 5.0% by weight (Example 2).

Comparative Example 1

A cosmetic composition was prepared in the same manner as in the above example, except that the extract of neck bark was not contained. Specific components and contents of this Comparative Example are as shown in the following [Table 7].

ingredient Example 1 (% by weight) Example 2 (% by weight) Comparative Example 1 (% by weight) Bark skin extract (Production Example 13) 5.0 - - Bark Skin Extract (Preparation 16) - 5.0 - glycerin 5.0 5.0 5.0 Lactic acid - - 5.0 Liquid paraffin 10.0 10.0 10.0 Polysorbate 60 2.0 2.0 2.0 Sorbitan sesquirololeate 1.0 1.0 1.0 Squalane 5.0 5.0 5.0 Beeswax 10. 10.0 10. Triethanolamine 0.5 0.5 0.5 Propylene glycol 1.0 1.0 1.0 Carboxyl Vinyl Polymer 1.5 1.5 1.5 incense a very small amount a very small amount a very small amount antiseptic a very small amount a very small amount a very small amount Purified water Remaining amount Remaining amount Remaining amount system 100 100 100

Experimental Example 6

<Skin patch (skin safety) test>

Human skin patch test of cosmetics containing the extract of Bark skin extract in 20 women in their 20s to 30s who have never had hypersensitivity to skin irritation and have no skin disease or skin allergy. Was done.

First the test site was wiped with 70% ethanol and dried. 15 μg of the prepared test substance was dropped in a Fin chamber (Finn chamber, 100 × 10, EPITEST, Finland), and then sealed in the forearm inner part of the test subject. The patch was marked for 24 hours, the patch was removed, and the test site was marked with a pen. After 1 hour and 24 hours after marking, the test site was observed using a magnifying glass (8MC-150, DAZOR, United States of America) to determine skin reaction (erythema and edema).

Skin reactions were determined according to the regulations of the International Contact Dermaitis Research Group (ICDRG). Evaluation criteria and scores of the skin response was calculated as shown in the following [Table], the average skin response (mean response rate) was calculated according to the following [Equation 1]. The results are shown in the following [Table 8].

            Evaluation Criteria and Score of Skin Response

Symbol score Evaluation standard - 0 No response ± 0.5 Faint or light erythema  +  One Boundary but weak erythema, edema and papules  ++ 2 Pronounced erythema, papules and vesicles  +++ 3 Severe erythema and alveolar, scleroderma

Test substance After 1 hour After 24 hours Reactivity (%) (n = 20)  ± + ++ ± + ++ Example 1 - - - - - - 0.00 Example 2 - - - - - - 0.00 Comparative Example 1 13 3 - 5 3 - 12.50

As shown in [Table 8], in the skin patch test, Comparative Example 1 containing lactinic acid showed strong skin irritation, but the cosmetic composition of Examples 1 and 2 of the present invention containing the dermis extract was skin No skin irritation showed excellent skin safety.

Experimental Example 7

<Measurement of skin wrinkle improvement effect>

In order to determine the skin wrinkle improvement effect of the cosmetic composition according to the above Examples and Comparative Examples, the cosmetic composition was applied to the human body as follows, and then the degree of skin wrinkle improvement was evaluated.

60 healthy women in their 30s and 40s were divided into three groups of 20 people, and the cosmetic composition according to Examples 1 and 2 and Comparative Example 1 was applied twice a day for 2 months. After that, the replica was made using silicon, and image analysis was performed by measuring the state of wrinkles with a visiometer (SV600, Courage + Khazaka electronic GmbH, Germany). The results are shown in the following [Table 9] and [Table 10]. Table 9 below is a result of analyzing the wrinkle image before use, Table 10 is a result of analyzing the wrinkle image after two months. In this case, the results in Table 9 and Table 10 below are average values for 20 people.

0 day Example 1 Example 2 Comparative Example 1 R1 3.007 3.012 3.010 R2 2.990 2.995 2.993 R3 1.142 1.134 1.137 R4 0.251 0.249 0.247 R5 0.375 0.369 0.368

60 day Example 1 Example 2 Comparative Example 1 R1 2.895 2.820 3.007 R2 2.480 2.108 2.991 R3 1.002 0.092 1.131 R4 0.228 0.115 0.246 R5 0.334 0.292 0.363

Above, in [Table 9] and [Table 10],

R1: difference between the highest and lowest of the wrinkle contour

R2: Average of R1 values after arbitrarily dividing the wrinkle contour by 5 spaces

R3: Highest value of R1 divided by 5

R4: Mean value of base line of wrinkled contour minus the value of the top and valley of each angle

R5 is the mean value of the baseline of the wrinkle contour, minus each wrinkled duct.

As shown in the [Table 9] and [Table 10], Examples 1 and 2 containing the extract of the bark dermis was found to be significantly improved skin wrinkle improvement compared to Comparative Example 1, in particular containing a large amount of the polymer In the case of Example 2 it was confirmed that the skin wrinkle improvement effect was greatly enhanced.

Experimental Example 8

<Measurement of skin elasticity improvement effect>

In order to determine the skin elasticity recovery effect of the cosmetic composition according to the above Examples and Comparative Examples, 10 healthy subjects in 30 to 40 subjects were measured by dividing the group into three groups of 10 people one. After applying twice a month, morning and evening, on the right side and the left side of the face, respectively, the skin elasticity after one month was measured. Skin elasticity was measured using a cutometer (MPA 580, Courage + Khazaka GmbH, Germany) as usual, and the elasticity of the facial area was measured to evaluate the improvement (%) by comparing before and after application. The results are shown in the following [Table 11], and the results are average values of improvement for each group.

Remarks Skin elasticity improvement (%) Example 1 38.4 Example 2 77.1 Comparative Example 1 5.7

As shown in [Table 11], Examples 1 and 2 containing the extract of the bark skin were found to be very improved in skin elasticity, as compared with the comparative example, especially in Example 2 containing a large amount of the polymer polymer It can be confirmed that the degree is a significant effect.

Hereinafter, the formulation example of the cosmetic composition containing the extract of neck peel according to the present invention is illustrated. However, the composition of the present invention is not intended to be limited to the following formulation examples.

Formulation Example 1

<Skin Lotion>

A skin lotion was prepared according to a conventional method with the ingredients and contents shown in the following [Table 12].

Raw material name Content (% by weight) Preparation Example 16 5.0 1,3-butylene glycol 5.2 Oleyl alcohol 1.5 ethanol 3.2 Polysorbate 20 3.2 Benzophenone-9 2.0 Carboxyl Vinyl Polymer 1.0 glycerin 3.5 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 2

<Lotion>

The lotion was prepared according to a conventional method with the ingredients and contents shown in the following [Table 13].

Raw material name Content (% by weight) Preparation Example 16 5.0 Cetostearyl alcohol 1.0 Glyceryl Monostearate 0.8 Sorbitan monostearate 0.3 Polysorbate 60 1.0 Mineral oil 5.0 Pyromethicone 3.0 Dimethicone 0.5 Allantoin 0.1 glycerin 5.0 Alcohol 2 Propylene glycol 3.0 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

[Formulation Example 3]

<Essence>

Essence was prepared according to a conventional method with the ingredients and contents shown in the following [Table 14].

Raw material name Content (% by weight) Preparation Example 16 5.0 1,3-butylene glycol 4.0 glycerin 3.0 Allantoin 0.5 Panthenol 0.1 EDTA-2Na 0.01 ethanol 5.0 Triethanolamine 1.5 Squalane 2.0 Beeswax 2.5 Polysorbate 60 3.5 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 2.5 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

[Formulation Example 4]

<Pack>

A pack was prepared according to a conventional method with the ingredients and contents shown in the following [Table 15].

Raw material name Content (% by weight) Preparation Example 16 5.0 Ethyl alcohol 3.0 EDTA-2Na 0.05 Propylene glycol 5.0 Glyceline 4.5 Cabopol 1.5 Polyoxide 0.2 antiseptic a very small amount incense a very small amount Purified water Remaining amount system 100

Formulation Example 5

<Massage cream>

A massage cream was prepared according to a conventional method with the ingredients and contents shown in the following [Table 16].

Raw material name Content (% by weight) Preparation Example 16 5.0 glycerin 4.0 Vaseline 3.5 Triethanolamine 0.5 Liquid paraffin 24.0 Squalane 3.0 Beeswax 2.1 Tocopheryl Acetate 0.1 Polysorbate 60 2.4 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 2.3 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 6

<Shampoo>

The shampoo was prepared according to a conventional method with the ingredients and contents shown in the following [Table 17].

Raw material name Content (% by weight) Preparation Example 16 5.0 Alkyl sulfate (30%) 25.0 Alkyl ether sulfate (30%) 20.0 Alkylamidobetaine (30%) 5.0 Propylene glycol 3.0 Lauramid 4.0 EDTA-2Na 0.1 Polyquaternium-10 0.05 NaCl 0.1 Citric acid 2.4 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

[Formulation Example 7]

<Body Cleanser>

A body cleanser was prepared according to a conventional method with the ingredients and contents shown in the following [Table 18].

Raw material name Content (% by weight) Preparation Example 16 5.0 Alkyl ether saccharide (30%) 30.0 Alkyl ether sulfosuccinate (30%) 20.0 Alkylamidobetaine (30%) 5.0 Propylene glycol 5.0 Betaine 5.0 Lauramiddiei 4.0 Cellulose gum 0.05 Hydrolyzed protein 0.1 Citric acid a very small amount NaCl a very small amount incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Claims (2)

In the cosmetic composition containing the extract of neck skin, The extract of the neck dermis is extracted from a mixed extraction solvent containing water, alcohols and glycols, The alcohol system is at least one selected from the group consisting of methanol, ethanol, propanol and butanol, The glycol system is at least one selected from ethylene glycol, propylene glycol and butylene glycol, The water, alcohol and glycol systems are mixed in a weight ratio of 2: 1 to 1 to obtain an inpolymer from the bark, The method for producing a cosmetic composition containing the extract of the bark of the neck, characterized in that the extract contains 10.0 ~ 17.4 ng / ml of the inpolymer. The extract of claim 1, wherein the extract is added to the mixed extract solvent in an amount of 10 to 30 times the weight of the extract, and then extracted for 5 to 20 hours at a temperature of 50 to 100 ° C. The manufacturing method of the cosmetic composition containing.