KR100538078B1 - Cosmetic Compositions Comprising Panax ginseng (C.A. MEY) Extract and Plant Extracts as Oriental Medicine for Preventing Skin Aging - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a herbal extract, more specifically, a composition for skin cell proliferation, collagen synthesis enhancement composition comprising wild ginseng, Macmundong, Bokryeong, soybean, cornus, lichen, licorice extract as an active ingredient It relates to a composition for inhibiting skin hypersensitivity reaction and a cosmetic composition for preventing skin aging.

Since the cosmetic composition of the present invention exhibits skin cell proliferation, collagen synthesis enhancement, skin hypersensitivity reaction, and moisturizing improvement without irritation to the skin, it is useful for preventing complex skin aging such as wrinkles, decreased elasticity, and reduced moisture retention. Do.

Description

Cosmetic composition for preventing skin aging containing wild ginseng extract and herbal extracts {Cosmetic Compositions Comprising Panax ginseng (C.A. MEY) Extract and Plant Extracts as Oriental Medicine for Preventing Skin Aging}

The present invention relates to a composition comprising a herbal extract, and more particularly, a composition for skin cell proliferation, a collagen synthesis enhancing composition, comprising wild ginseng, mackmundong, bokyeong, wild herbs, cornus, lichen and licorice extract as an active ingredient. It relates to a composition for inhibiting skin hypersensitivity reaction and a cosmetic composition for preventing skin aging.

As the largest organ of the human body, which occupies about 16% of the body's volume, the skin is in direct contact with the external environment and is an important protective barrier that protects the body from many deadly harmful factors, such as temperature, humidity, and ultraviolet radiation, which are likely to enter the body. Play a role. However, as they age, skin cells are damaged due to various contaminants, strong ultraviolet rays, stress and malnutrition, and cell proliferation is not performed properly, resulting in wrinkles, loss of elasticity and keratinization. .

Originally, normal healthy skin has a protective system that protects the skin from these external damaging factors, but due to continuous external stimulus, the protective effect of protecting the skin from external damaging factors is lost, and the cell protection and proliferation ability is poor. Skin aging phenomena, such as reduced skin elasticity and wrinkles appear.

In addition, the loss of skin's moisturizing power and loss of turn over function is also associated with wrinkle formation and skin elasticity, which is a kind of phenomenon caused by skin aging. As we age, the amount of collagen, mucopolysaccharides and hyaluronic acid in the matrix, which play an important role in skin elasticity, decreases, while the amount of insoluble collagen increases, so the dermis It becomes difficult to keep the skin hydrated, and the skin becomes rough. In addition, with age, the function of sebaceous glands and sweat glands decreases, so that the amount of sebum and sweat gradually decreases, the sebaceous membrane is not properly produced, and the protection for skin moisturization is lost. Translucence is lost, the elasticity of skin proteins is reduced and wrinkles are created. In other words, the loss of moisturizing of the skin is a major symptom of aging along with decreased skin elasticity and wrinkle formation and is closely related.

In addition, when human skin cells are exposed to ultraviolet rays (UV-B, UV-A), it is known that active oxygen species, which are a major cause of skin damage, are generated and wrinkles are induced.

Collagen and elastic fibers (elastin) present in the dermal layer of the skin structure is the main protein that controls the elasticity of the skin. Biosynthesis of collagen fibers is influenced by internal and external factors of the skin. Internally, natural aging (reduction of estrogen) decreases cellular activity resulting in the reduction of collagen fibers. External factors include active oxygen (free radical type) generated by excessive irradiation of UV rays and stress, which react with the -SH group of the protein to inhibit the activity of the enzyme or decompose collagen and elastin (Matrix Metalloproteinases: MMPS). ) Increases the expression of collagenase, which leads to aging of the skin wrinkles and reduced elasticity.

In order to suppress the skin aging or to slow the progression, cosmetics or skin protectants containing various skin protection ingredients such as retinol, retinoid, vitamin C, flavonoids and tocopherol have been developed. However, such cosmetics or skin protectors have a problem in that the potency is low in the final formulation, the efficacy is slight, and the skin irritation is severe.

Korean Patent Application No. 1997-60849 discloses a skin anti-aging cosmetic composition containing serine of retinol, alpha hydroxy acid and vitamins, especially beta hydroxy acid, and Korean Patent Application No. 1998-18636 discloses a polyamino polymer. An anti-aging cosmetic composition comprising an active ingredient is disclosed. In addition, Korean Patent Application No. 1994-7002880 discloses a synergistic cosmetic composition as an antioxidant containing ginkgo extract and at least one polyphenol derivative, and Korean Patent Application No. 1996-69236 discloses L-2- Disclosed is a skin anti-aging composition comprising an oxothiazolidine-4-carboxylic acid derivative.

Currently, research is being actively conducted for the development of cosmetics that can protect skin cells from the external environment such as ultraviolet light, activate cell proliferation ability, activate cell functions, and impart smooth moisturizing power. However, the conventional results are insignificant, and there is no known cosmetic composition that complexly resolves all the various aging phenomena such as wrinkles, decreased elasticity, and loss of moisturizing ability.

Throughout this specification, numerous patent documents are referenced and their citations are indicated. The disclosures of the cited patent documents are incorporated herein by reference in their entirety, so that the level of the technical field to which the present invention belongs and the contents of the present invention are more clearly explained.

The present inventors have diligently researched to develop anti-aging skin, that is, skin protection cosmetic composition using medicinal herbs and herbal medicines. It has an effect of inhibiting reaction and improving skin moisturizing power, and cosmetics containing the herbal extract as an active ingredient have excellent skin anti-aging effect by eliminating complex skin aging phenomenon such as wrinkle generation, elasticity reduction and loss of moisturizing ability without skin irritation. By confirming that the skin can be effectively protected by inhibiting hypersensitivity reactions, the present invention has been completed.

Therefore, it is an object of the present invention to provide a composition for skin cell proliferation.

Another object of the present invention to provide a composition for enhancing collagen synthesis.

Another object of the present invention to provide a composition for inhibiting skin hypersensitivity.

Another object of the present invention to provide a complex skin anti-aging cosmetic composition without skin irritation and side effects.

According to one aspect of the present invention, the present invention is a wild ginseng ( Panax ginseng (CAMEY)), Ligupe platyphylla Wang et Tang, Poria cocos Wolf, Dioscorea opposita Thunb., Cornus officinalis Sieb . et Zucc), Rehmannia glutinosa Liboschtz var. and Licorice ( Glycyrrhiza glabra L.) extracts are provided as a composition for skin cell proliferation.

Wild ginseng contained as an active ingredient in the composition of the present invention has been known as a mysterious medicinal herb as a rare plant that has been treated as a mysterious elixir from ancient times, and is a mystery of rareness and medicinal efficacy. Wild ginseng, which grows wild in deep valleys and deep valleys, is the most precious and valuable herb, especially natural wild ginseng, which is increasingly rarely found and is known as a mysterious elixir found by a few simnies.

Morphological characteristics of wild ginseng First, the length of the brain head is significantly longer than the cultivated ginseng is usually 6 ~ 9㎝ or more. Second, the root is a slightly dark white rather than yellowish yellow like cultivated ginseng, and the shape of the rim of the ridge to the upper part or the root of the fuselage is rooted. Third, wild ginseng has longer brain heads, and the newly formed roots grow in large parts of the long brain head to form aggregate roots like dahlias, but they have less weight than cultivated ginseng. In addition, wild ginseng has long roots, so cultivated ginseng is around 30cm, but wild ginseng is longer than 1m. The peculiarity of wild ginseng is that it grows while adapting to the natural climate and environment, so there are few long leaves after 10 years. And the back side of the leaves is silver, characterized by thick leaflets and leaflets, and short flower scenes.

In general, the benefits of ginseng include improved blood circulation, hematopoiesis, cardiac depression, anti-aging, normalized blood pressure, normalized decreased liver function, gastrointestinal disease, diabetes treatment, cancer prevention and healing, anti-fatigue, stress relief, allergy It is known to improve the constitution, treat cold symptoms, relieve menopausal disorders, treat rheumatism, strengthen energy, promote brain activity, improve eyesight, improve skin care and hair, detoxify alcohol and strengthen immune function.

Wild ginseng has a higher content of active ingredients than ginseng and is known to have high drug efficacy. Proteins, amino acids, nucleic acids and alkaloids as nitrogen-containing compounds, including saponins as glycosides, fatty acids, essential oils, polyacetylene, phenolic compounds, phytosterols and terpenoids as sugars, monosaccharides as sugars, It contains a wide variety of ingredients, including oligosaccharides, polysaccharides and pectins, and vitamins and minerals.

In general, saponin components are widely distributed in the plant kingdom (about 750 plants). Saponin is the name given to the microscopic foaming of soap in aqueous solutions. In general, saponin lowers the surface tension of water, so it foams easily and shows hemolytic action. However, it was found that saponin of wild ginseng is not only weak and toxic but also has little hemolytic action. In addition, the saponin component of wild ginseng has a completely different chemical structure from the saponins found in other plants, and in order to distinguish the saponins of wild ginseng from other plant-based saponins, it is called ginsenoside, meaning Ginseng glycoside. Call.

Raw materials other than wild ginseng are Liriope platyphylla Wang et Tang, Pokyeong ( Poria cocos Wolf), Chinese medicine ( Dioscorea opposita Thunb.), Cornus officinalis Sieb . Et Zucc, Rehmannia glutinosa Liboschtz var. Glycyrrhiza glabra L.) also has the effects of skin exfoliation, antibacterial, anti-inflammatory, wound healing, excellent water retention and blood circulation.

The herbal extract of the present invention can be obtained using various extraction methods known in the art, and preferably, (a) water, (b) anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, propanol). , Butanol, etc.), (c) a mixed solvent of the lower alcohol and water, (d) acetone, (e) ethyl acetate, (f) chloroform or (g) 1,3-butylene glycol as an extraction solvent. can do.

On the other hand, it will be apparent to those skilled in the art that the extract of the present invention can be obtained not only the above-described extraction solvent but also herbal extracts having substantially the same effect using other extraction solvents.

In addition, the extract of the present invention includes not only the extract by the above-described extraction solvent, but also the extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also included in the herbal extract of the present invention.

The herbal extract of the present invention may be prepared in powder form by an additional process such as distillation under reduced pressure and freeze drying or spray drying.

Referring to the preparation method of wild ginseng and herbal extract with reference to the preparation example of the present invention as follows: Wild ginseng and herbal extract is 10 g, wild ginseng 20 g, wild medicinal 20 g, licorice 20 g, Bokryeong 10 g, Macmundong 10 g And it is made by adding water to 10 g of sulfuric acid to 1 l, and then about 1 hour at 70 ℃ month, filtered and cooled to room temperature, then aged at low temperature at 4 ℃ 7 days and filtered again.

In a preferred embodiment of the present invention, the content of wild ginseng and herbal extract is 0.0001 to 10.0% by weight, more preferably 0.0005 to 10.0% by weight, and most preferably 0.005 to 5.0% by weight based on the total composition.

As used herein, the term "skin cells" refers to cells that form skin, and includes fibroblasts and keratinocytes.

According to another aspect of the present invention, the present invention provides a composition for enhancing collagen synthesis comprising wild ginseng, cornus milk, Chinese medicine, licorice, Macmundong, Bokryeong, and sulfuric acid extract as an active ingredient.

Since the composition of the present invention includes the above-mentioned wild ginseng and herbal extract as an active ingredient, in order to avoid excessive complexity of the present specification, duplicated descriptions are omitted.

According to another aspect of the present invention, the present invention provides a cosmetic composition for inhibiting skin hypersensitivity reactions comprising wild ginseng, cornus, medicinal herbs, licorice, Macmun-dong, Bokryeong, and even sulfur extract as an active ingredient.

Since the composition of the present invention includes the above-mentioned wild ginseng and herbal extract as an active ingredient, in order to avoid excessive complexity of the present specification, duplicated descriptions are omitted.

The composition of the present invention effectively inhibits excessive skin hypersensitivity, thereby preventing the skin from hypersensitivity to ambient irritation. In particular, ultraviolet light irradiation effectively suppresses contact hypersensitivity to the skin.

According to another aspect of the present invention, the present disease provides a cosmetic composition for preventing skin aging comprising wild ginseng, cornus, medicinal herbs, licorice, Macmun-dong, Bokryeong, and sulfuric acid extract as an active ingredient.

Since the composition of the present invention includes the above-mentioned wild ginseng and herbal extract as an active ingredient, in order to avoid excessive complexity of the present specification, duplicated descriptions are omitted.

Preferably, the composition of the present invention prevents skin aging by simultaneously achieving wrinkle improvement, skin elasticity enhancement and skin moisturization improvement. Until now, the cosmetic composition which prevents skin aging by exhibiting all the above-mentioned effects is not known.

The components included in the cosmetic composition of the present invention include components commonly used in cosmetic compositions in addition to wild ginseng extract and herbal extract as active ingredients, and include, for example, conventional agents such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings. Adjuvants, and carriers.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations and sprays, and the like, but are not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The cosmetic composition of the present invention has the use of skin anti-aging. As used herein, the term "anti-aging of skin" has the meaning encompassing comprehensively the prevention and treatment of skin aging.

The cosmetic composition of the present invention prevents skin aging through the improvement of skin wrinkles and the improvement of skin elasticity and the moisturizing ability by the proliferation of fibroblasts and keratinocytes, the enhancement of collagen synthesis, and this fact is specified in the following examples. have. Thus, skin aging prevention (ie, skin protection), which is the use of the present invention, is construed to include the use of wrinkle improvement, skin elasticity improvement, and moisturizing improvement.

The present invention also provides a cosmetic composition for inhibiting skin hypersensitivity.

As such, the composition of the present invention is able to completely protect the skin through the prevention of skin aging and skin hypersensitivity reaction.

On the other hand, the cosmetic composition of the present invention has the advantage of almost no skin irritation and side effects.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it is to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. Will be self-evident.

Preparation Example 1: Preparation of wild ginseng and herbal extract

Wild ginseng and herbal extracts were made of 1 g of 10 g of wild ginseng, 20 g of corn cod, 20 g of cabbage, 20 g of licorice, 10 g of Bokryeong, 10 g of Macmundong, and 10 g of sulfuric acid. After filtration, the mixture was cooled to room temperature and then aged at low temperature at 4 ° C. for 7 days and filtered again.

According to a preferred embodiment of the present invention, the content of wild ginseng and herbal extract is 0.0001 to 10.0% by weight, more preferably 0.0005 to 10.0% by weight, most preferably 0.005 to 5.0% by weight based on the total cosmetic composition. When the content of wild ginseng and herbal extracts is less than 0.0001% by weight, no obvious anti-aging effect, in particular, wrinkle improvement cannot be expected. In addition, when the content of wild ginseng and herbal extracts exceeds 10.0% by weight, there is no apparent increase in effect as the content increases.

Experimental Example 1 Fibroblast Proliferation Effect

Human normal fibroblasts (Korea Cell Line Bank) were seeded to 1 × 10 ⁴ cells in each well of a 96-well microplate and incubated at 37 ° C. for 24 hours in DMEM medium (sigma). . Subsequently, the experimental group in which the wild ginseng and herbal extracts were replaced with serum-free DMEM medium containing a final concentration of 0.005%, 0.01%, 0.05%, 0.1%, and 1%, and the serum-free DMEM medium containing no wild ginseng and herbal extracts The replaced control was further incubated for 24 hours. Then, 10 μl of MTT solution (3- (4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide: 5 mg / ml) was added to each, and incubated for 4 hours. After that, the medium was removed and 100 μl of dimethyl sulfoxide solution per well was added and shaken for 20 minutes. The absorbance of the supernatant was measured at 570 nm using a microplate reader (Molecular Devices, USA). The measured value was substituted into Equation 1 below to calculate the cell proliferation effect, and the results are shown in Table 1 below.

Cell growth effect (%) = [(absorbance at extract treatment-absorbance of control) / absorbance of control] × 100

extract(%) Absorbance Cell proliferation effect (%) Control 0.330 - 0.005 0.353 7.2 0.001 0.391 18.5 0.05 0.459 39.0 0.1 0.531 60.8 One 0.604 83.1

As can be seen in Table 1, wild ginseng and herbal extracts show a fibroblast proliferation effect as compared to the control group not treated with wild ginseng and herbal extracts.

Experimental Example 2 keratinocyte proliferation effect

Using keratinocytes, the proliferative effect of keratinocytes was measured in the same manner as in Experimental Example 1, and the results are shown in Table 2.

extract(%) Absorbance Cell proliferation effect (%) Control 0.324 - 0.005 0.337 4.1 0.001 0.361 11.5 0.05 0.387 19.3 0.1 0.425 31.3 One 0.527 62.8

Experimental Example 3 Collagen Synthesis Enhancement Effect

Human normal fibroblasts (Korea Cell Line Bank) were seeded in each well of a 96-well microplate to be 2 × 10 ⁴ cells and incubated for 24 hours at 37 ° C. in DMEM medium. Subsequently, the experimental group in which the prepared wild ginseng and herbal extracts were replaced with serum-free DMEM medium containing a final concentration of 0.005, 0.01, and 0.05% and the control group in which the wild ginseng and herbal extracts were replaced with serum-free DMEM medium that did not contain wild ginseng and herbal extracts were 24. Further incubation for an hour, ascorbic acid (50 g / ml) was added to promote collagen synthesis, followed by another 24 hours. Each well was then washed and replaced with serum-free DMEM medium and incubated for an additional 24 hours. After incubation, the supernatant of each well was collected and the amount of newly synthesized collagen was measured using a kit (Takara, Japan) for the amount of procollagen (procollagen) type I C-peptide (PICP). PICP amount was converted to ng / 2 × 10 ⁴ cells, and the results are shown in Table 3 below.

% Of concentration (v / v) Collagen Synthesis (ng / 2 x 10 ⁴ ) Control 153 0.005 189 0.01 204 0.05 250

As can be seen in Table 3, it can be seen that the synthesis of collagen increases as the concentration of wild ginseng and herbal extract increases, whereby the wild ginseng and herbal extract exhibits excellent collagen synthesis promoting effect.

Experimental Example 4 skin hypersensitivity inhibition test

After shaving the abdomen in 8-10 week old C57BL / 6 mice, irradiating UV B wavelength (UVB) at 2.4 kJ / m2 with FS-20 sun lamp results in contact hypersensitivity. . After irradiating UV, a substance having an immunosuppressive effect is applied to the site, and the immunosuppression phenomenon is measured in comparison with a control group not coated with the substance. Therefore, the wild ginseng and herbal extract of the present invention was applied after UV irradiation, and then sensitized with 3% trinitrochlorobenzene in the UV irradiation area after 3 days. After 6 days, 1% trinitrogen chloride was applied to the ears of mice and edema status was measured. The skin hypersensitivity inhibitory effect was calculated by the following Equation 2 and the results are shown in Table 4 below.

[(Production example application and ear edema thickness of UVB irradiated group-ear edema thickness of UVB irradiated group) / (ear edema thickness of control group-ear edema thickness of UVB irradiated group)] × 100

% Of concentration (v / v) Skin hypersensitivity inhibitory effect (%) Control - 0.005 7.6 0.01 15.4 0.05 33.9

As can be seen in Table 4, it can be seen that the herbal extract of the present invention increases the skin hypersensitivity inhibitory effect concentration-dependently.

Examples and Comparative Examples

To the flexible cosmetics cosmetic composition having a composition ratio of Table 5 below to prepare an example containing the wild ginseng and herbal extract 5% (v / v) and a comparative example not containing the wild ginseng and herbal extract in Experimental Example 5 Was used.

ingredient Example (% by weight) Comparative Example Wild ginseng and herbal extract 0.5 0 glycerin 5.1 5.1 Propylene glycol 4.2 4.2 Tocopheryl Acetate 3.0 3.0 Liquid paraffin 4.6 4.6 Triethanolamine 1.0 1.0 Squalane 3.1 3.1 Macadamia Nut Oil 2.5 2.5 Polysorbate 60 1.6 1.6 Sorbitan sesquirrolate 1.6 1.6 Propylparaben 0.6 0.6 Carboxyl Vinyl Polymer 1.5 1.5 incense a very small amount a very small amount antiseptic a very small amount a very small amount Purified water Remaining amount Remaining amount system 100 100

Experimental Example 5: Moisturizing Effect

In order to evaluate the moisturizing power of the Examples and Comparative Examples, the water retention capacity and the water evaporation amount of the skin to which each cosmetic was applied were measured as follows.

Experimental Example 5-1: Measurement of Water Retention Capacity

In the constant temperature and humidity room of 22 ° C. and 45% relative humidity, the above-mentioned Examples and Comparative Examples were applied to a certain amount (0.03 g / 16 cm 2) in the inner half of 30 test subjects, and before, 1 hour after application and 2 hours after application The moisture content of was measured and normal skin without any treatment was used as a control. As a measuring device, a moisture content meter (corneometer CM820, Conrage + Khazaka, Germany) was used to measure the moisturizing power by measuring the electric capacity of the skin according to the moisture content of the skin. The results are shown in Table 6 below.

division Example Comparative Example Control Skin electrical conductivity before application 50 50 50 Skin electrical conductivity 1 hour after application 87 75 50 Skin electrical conductivity 2 hours after application 75 59 50

Experimental Example 5-2: Measurement of Water Evaporation Amount

Tape stripping was applied to the upper arm region of the arm to weaken the skin's protective film. After applying each cosmetic to the subject in the same manner as in Experiment 5-1, the water vaporizer (TEWA meter, koln, Germany) Federated water evaporation was measured 5 times at 1 minute intervals every 12 hours using the Federal Bureau of Laboratories. The results are shown in Table 7 below.

division Example Comparative Example Control Moisture evaporation after 0 hours 4.0% 4.0% 0.0% Water evaporation after 12 hours -9.1% -5.5% -0.1% Water evaporation after 24 hours -7.2% -1.0% 0.1% Water evaporation after 36 hours -5.0%  2.5% 0.1% Water evaporation after 48 hours -2.3%  3.0% 0.0%

As shown in the results of Experimental Examples 5-1 and 5-2, the Example of the present invention has a higher electrical conductivity of the skin and a smaller amount of water evaporation than the Comparative Example. Therefore, it can be seen that the cosmetic composition containing the extract of the present invention exhibits an excellent moisturizing effect.

Experimental Example 6: Skin Irritation Test

In order to examine the skin irritation of wild ginseng and herbal extracts prepared according to the preparation example, a predetermined amount (about 0.1 g) of each sample was placed at 5 × 20 cm in the lower part of both arms of 30 healthy adult males and females. After patching for a time, 1 hour and 24 hours after removal, the skin condition change was read visually according to the following criteria, and the stimulation rate was calculated according to the following equation (3). The experimental results are shown in Table 8 below.

Criteria

-: No erythema or unusual phenomenon

+-: Slightly reddish

+: Significantly redder than the surroundings

++: reddens and swells more heavily

Stimulation rate (%) = [((+-) number × 1 + (+) number × 2 + (++) number × 3) / (total number × 3)] × 100

Production Example Judgment result Stimulation rate (%) ++ + +- - One - - One 29 1.1

As can be seen from the skin irritation test results of Table 8, it can be seen that the wild ginseng and herbal extract of the present invention has no skin irritation.

Formulation Example 1

Formulation examples of the flexible lotion (skin) in the cosmetics comprising the wild ginseng and herbal extracts obtained above are as follows.

ingredient Content (% by weight) Wild ginseng and herbal extract 0.5 1,3-butylene glycol 5.2 Oleyl alcohol 1.5 ethanol 3.2 Polysorbate 20 3.2 Benzophenone-9 2.0 Carboxyl Vinyl Polymer 1.0 glycerin 3.5 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 2

Formulation examples of the milk lotion in the cosmetic comprising the extract of wild ginseng and herbal medicine obtained above are as follows.

ingredient Content (% by weight) Wild ginseng and herbal extract 0.6 glycerin 5.1 Propylene glycol 4.2 Tocopheryl Acetate 3.0 Liquid paraffin 4.6 Triethanolamine 1.0 Squalane 3.1 Macadamia Nut Oil 2.5 Polysorbate 60 1.6 Sorbitan sesquirrolate 1.6 Propylparaben 0.6 Carboxyl Vinyl Polymer 1.5 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 3

Formulation examples of the nourishing cream in the cosmetic composition containing the wild ginseng and herbal extract obtained above are as follows.

ingredient Content (% by weight) Wild ginseng and herbal extract 1.0 glycerin 4.0 vaseline 3.5 Triethanolamine 2.1 Liquid paraffin 5.3 Squalane 3.0 Beeswax 2.6 Tocopheryl Acetate 5.4 Polysorbate 60 3.2 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 3.1 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 4

Formulation example of a massage cream in a cosmetic containing wild ginseng and herbal extract of the present invention is as follows.

ingredient Content (% by weight) Wild ginseng and herbal extract 0.5 glycerin 4.0 vaseline 3.5 Triethanolamine 0.5 Liquid paraffin 24.0 Squalane 3.0 Beeswax 2.1 Tocopheryl Acetate 0.1 Polysorbate 60 2.4 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 2.3 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 5

Formulation examples of the pack in the cosmetic containing the wild ginseng and herbal extracts obtained above are as follows.

ingredient Content (% by weight) Wild ginseng and herbal extract 1.0 Ethyl alcohol 3.0 EDTA-2Na 0.02 Propylene glycol 5.1 Glyceline 4.5 Cabopol 1.0 Polyoxide 0.1 antiseptic a very small amount incense a very small amount Purified water Remaining amount system 100

As described above, the present invention is a skin cell proliferation composition, collagen synthesis enhancement composition, skin hypersensitivity composition and skin aging including wild ginseng extract, other than Mengmundong, Bokryeong, medicinal herbs, cornus, lichens, licorice extract as an active ingredient It provides a cosmetic composition for prevention. Since the cosmetic composition of the present invention exhibits skin cell proliferation, collagen synthesis enhancement, and moisturizing improvement effect, it has an excellent effect on preventing complex skin aging such as wrinkle generation, elasticity reduction, and moisturizing ability, and almost no irritation to the skin. none.

Claims (6)

delete delete A cosmetic composition for preventing skin aging comprising wild ginseng, cornus, medicinal herb, licorice, macmundong, bokyeong, and huangwang extract as an active ingredient. Cosmetic composition for inhibiting skin hypersensitivity reactions, including wild ginseng, cornus, mountain medicine, licorice, ganmun-dong, Bokryeong, and gohwang extract. The cosmetic composition for preventing skin aging according to claim 3, wherein the composition prevents skin aging by simultaneously achieving wrinkle improvement, skin elasticity improvement and skin moisturization improvement. The cosmetic composition according to claim 3 or 4, wherein the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation And a cosmetic composition for preventing skin aging, characterized in that it has a formulation selected from the group consisting of a spray.