KR100570094B1 - Oriental Skin Care Cosmeceutical Composition for Oral Administration Containing Galenical Extract - Google Patents

Abstract

The present invention relates to an oral skin anti-aging cosmetic composition containing an herbal extract as an active ingredient, and more specifically, a composition for growing skin cells containing ginseng, cornus, cauliflower, licorice, bokryeong and turmeric extract as an active ingredient, It relates to a collagen synthesis enhancing composition, skin moisturizing composition and oral skin anti-aging cosmetic composition. The oral cosmetic composition of the present invention effectively prevents complex skin aging through skin cell proliferation, collagen synthesis enhancement and skin moisturization improvement.

Herbal extract, skin aging, oral skin anti-aging cosmetic composition

Description

Oriental Skin Care Cosmeceutical Composition for Oral Administration Containing Galenical Extract}

The present invention relates to an oral skin anti-aging cosmetic composition comprising a herbal extract.

As the largest organ of the human body, which occupies about 16% of the body's volume, the skin is in direct contact with the external environment and is an important protective barrier that protects the body from many deadly harmful factors, such as temperature, humidity, and ultraviolet radiation, which are likely to enter the body. Play a role. However, as they age, skin cells are damaged due to various contaminants, strong ultraviolet rays, stress and malnutrition, and cell proliferation is not performed properly, resulting in wrinkles, loss of elasticity and keratinization. .

Originally, normal healthy skin has a protective system that protects the skin from these external damaging factors, but the protective effect of protecting the skin from external damaging factors is lost by continuous external stimuli, and the ability to protect and proliferate cells. Apart from this, skin aging phenomena such as reduction of skin elasticity and wrinkle formation appear.

In addition, the loss of skin's moisturizing power and loss of turn over function is also associated with wrinkle formation and skin elasticity, which is a kind of phenomenon caused by skin aging. As we age, the amount of collagen, mucopolysaccharides and hyaluronic acid in the matrix, which play an important role in skin elasticity, decreases, while the amount of insoluble collagen increases, so the dermis It becomes difficult to keep the skin hydrated, and the skin becomes rough. In addition, with age, the function of sebaceous glands and sweat glands decreases, so that the amount of sebum and sweat gradually decreases, the sebaceous membrane is not properly produced, and the protection for skin moisturization is lost. Translucence is lost, the elasticity of skin proteins is reduced and wrinkles are created. In other words, the loss of moisturizing of the skin is a major symptom of aging along with decreased skin elasticity and wrinkle formation and is closely related to it.

In addition, when human skin cells are exposed to ultraviolet rays (UV-B, UV-A), it is known that active oxygen species, which are a major cause of skin damage, are generated and wrinkles are induced.

Collagen and elastic fibers (elastin) present in the dermal layer of the skin structure is the main protein that controls the elasticity of the skin. Biosynthesis of collagen fibers is affected by internal and external factors of the skin. Internally, aging decreases the activity of cells due to estrogen reduction, thereby reducing collagen fibers. External factors include free radicals generated by excessive irradiation of UV rays and stress, that is, free radicals react with the -SH group of proteins to inhibit the activity of enzymes or to degrade collagen and elastin (Matrix Metalloproteinases: MMPS). ) Increases the expression of collagenase, which leads to aging of the skin wrinkles and reduced elasticity.

Therefore, it is required to develop excellent materials that can effectively prevent or suppress skin aging by activating skin cells whose functions are degraded by various factors to perform skin metabolism smoothly.

The researcher of the present invention has completed the present invention by discovering that the herbal extract of the present invention has an excellent effect on preventing skin aging, as a result of intensive efforts to solve the above-described needs of the art.

Accordingly, it is an object of the present invention to provide a composition for propagating skin cells containing an herbal extract as an active ingredient.

Another object of the present invention to provide a composition for enhancing collagen synthesis containing a herbal extract as an active ingredient.

Another object of the present invention to provide a composition for moisturizing the skin containing a herbal extract as an active ingredient.

Another object of the present invention to provide an oral skin anti-aging cosmetic composition containing an herbal extract as an active ingredient.

Other objects and advantages of the present invention will become apparent from the following detailed description and claims.

According to an aspect of the present invention, the present invention is a ginseng ( Panax ginseng ), Pokyeong ( Poria cocos (F _R. ) W _OLF ), _{medicinal herb} ( Dioscorea opposita T _HUMB .), Cornus officinalis S _IEB . Et Z _UCC. ), A composition for proliferating skin cells (fibroblasts or keratinocytes) containing the extract of Rehmannia glutinosa Liboschtz var. And licorice ( Glycyrrhiza glabra L.) as an active ingredient.

According to another aspect of the present invention, the present invention provides a composition for enhancing collagen synthesis comprising ginseng, cornus, cauliflower, licorice, Bokryeong, and sulfuric acid extract as an active ingredient.

According to another aspect of the present invention, the present invention provides a skin moisturizing composition comprising ginseng, cornus, medicinal herb, licorice, Bokryeong, and even sulfur extract as an active ingredient.

According to another aspect of the present invention, the present invention provides a cosmetic composition for preventing oral skin aging comprising ginseng, cornus, medicinal herb, licorice, Bokryeong, and sulfur extract as an active ingredient.

Ginseng contained as an active ingredient in the composition of the present invention is known to be treated as a mysterious elixir from ancient times. In general, the benefits of ginseng include improved blood circulation, hematopoiesis, cardiac depression, anti-aging, normalized blood pressure, normalized decreased liver function, gastrointestinal disease treatment, diabetes treatment, cancer prevention and healing, anti-fatigue, stress relief, allergy It is known to be effective in improving sexual constitution, treating cold symptoms, relieving menopausal disorders, treating rheumatism, strengthening energy, promoting brain activity, improving eyesight, improving skin and hair, detoxifying alcohol and enhancing immune function.

Its components include saponins, glycosides, proteins, amino acids, nucleic acids and alkaloids, fatty acids, essential oils, polyacetylenes, phenolic compounds, and phytosterols. ) And terpennoides, sugars such as monosaccharides, oligosaccharides, polysaccharides and pectins, and vitamins and minerals.

In general, saponin components are widely distributed in the plant kingdom (about 750 kinds of plants). Saponin is the name given to the microscopic foaming of soap in aqueous solutions. In general, saponin lowers the surface tension of water, so it foams easily and shows hemolytic action. Ginseng saponins are not only weak and nontoxic, but have been found to have little hemolytic action. In addition, the saponin component of ginseng is completely different in chemical structure from the saponins found in other plants, and Ginsenoside means Ginseng Glycoside to distinguish ginseng saponins from other plant-based saponins. It is called.

Poria cocos (F _R. ) W _OLF , Cornus officinalis S _IEB . Et Z _UCC. And Licorice ( Glycyrrhiza glabra L.) also exfoliate skin, antibacterial, anti-inflammatory, promote wound healing and improve Efficacy in retaining moisture and promoting blood circulation.

The composition of the present invention exhibits a synergistic effect due to the mixing composition of the main active ingredients as described above, and thus has an excellent effect on skin aging symptoms.

The herbal extracts of the present invention can be prepared according to conventional methods known in the art, i.e., under conventional temperature conditions, using conventional solvents.

The solvent for preparing the herbal extract of the present invention is (a) water, (b) anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, propanol, butanol, etc.), (c) the lower alcohol and water Mixed solvents, (d) acetone, (e) ethyl acetate, (f) chloroform, and (g) 1,3-butylene glycol. Most preferably water is used as the extraction solvent. In addition, the extraction temperature for producing the herbal extract of the present invention is preferably 50-85 ° C, more preferably about 70 ° C.

Referring to the preparation method of the herbal extract with reference to the preparation example of the present invention is as follows: add water to the appropriate amount of ginseng, cornus oil, cauliflower, licorice, Bokryeong and sulfuric acid, and heated at about 70 ℃ for about 1 hour Next, the mixture is filtered, cooled to room temperature, then aged at low temperature at about 4 ° C. for 7 days and filtered again.

On the other hand, the herbal extract of the present invention includes not only the extract by the above-described extraction method, but also the extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using ultrafiltration membranes having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also included in the herbal extract of the present invention.

The herbal extract of the present invention may be prepared in powder form by an additional process such as distillation under reduced pressure and freeze drying or spray drying.

According to a preferred embodiment of the present invention, the content of the herbal extract is 0.0001 to 10.0% by weight, more preferably 0.0005 to 10.0% by weight, and most preferably 0.005 to 5.0% by weight based on the total weight of the total composition. . If the content of the herbal extract is less than 0.0001% by weight, it is not expected to have a clear skin anti-aging effect, in particular, wrinkle improvement. In addition, when the content of the herbal extract exceeds 10.0% by weight, there is no apparent increase in effect as the content increases.

As used herein, the term "skin cells" refers to cells that form skin and includes fibroblasts and keratinocytes.

The oral cosmetic composition of the present invention prevents skin aging by simultaneously achieving wrinkle improvement, skin elasticity enhancement and skin moisturizing. To date, the cosmetic composition for preventing skin aging by exhibiting all three effects described above is not known.

When the composition of the present invention is made into a cosmetic composition, in addition to the above-mentioned natural extract as an active ingredient, it includes components conventionally used in cosmetic compositions, for example, such as antioxidants, stabilizers, solubilizers, vitamins, pigments and perfumes. Phosphorus aids, and carriers.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations and sprays, and the like, but are not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

On the other hand, oral skin anti-aging cosmetic composition of the present invention as an active ingredient, in addition to the above-described herbal extracts include ingredients commonly used in food compositions or oral medicine, for example, antioxidants, stabilizers, solubilizers, vitamins, pigments And conventional adjuvants such as perfumes, and carriers. Carriers included in the oral cosmetic composition of the present invention are commonly used in the preparation, lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, calcium silicate, Microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, and the like. . Oral cosmetic composition of the present invention may further comprise a lubricant, wetting agent, sweetener, flavoring agent, emulsifier, suspending agent, preservative, etc. in addition to the above components.

Oral food compositions of the present invention may be prepared in unit dose form or formulated in unit dosage form by formulating with a carrier and / or excipient, according to methods readily available to those of ordinary skill in the art. It may be prepared by incorporation into a container. In this case, the formulation may be in the form of a solution, suspension or emulsion in an oil or an aqueous medium, or may be in the form of extracts, powders, granules, tablets or capsules, and may further include a dispersant or stabilizer.

The oral cosmetic composition of the present invention has the use of skin anti-aging. As used herein, the term "anti-aging of skin" has the meaning encompassing comprehensively the prevention and treatment of skin aging.

The food composition of the present invention prevents skin aging through the proliferation of fibroblasts and keratinocytes, the improvement of skin wrinkles and the improvement of skin elasticity by the enhancement of collagen synthesis, and the improvement of moisturizing power, and the facts are described in the following examples. It is.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. .

Preparation Example 1: Preparation of herbal extract

The herbal extract was made into 1 l of water to 10 g of ginseng, 20 g of cornus oil, 20 g of acidic acid, 20 g of licorice, 10 g of Bokryeong and 10 g of sulfuric acid. After filtration, the mixture was cooled to room temperature and then aged at low temperature at 4 ° C. for 7 days and filtered again.

Experimental Example 1 Fibroblast Proliferation Effect

Human normal fibroblasts (Fibroblast, Korea Cell Line Bank) were seeded to 1 × 10 ⁴ cells in each well of a 96-well microplate and incubated at 37 ° C. for 24 hours in DMEM (Sigma). It was. Subsequently, the serum-free DMEM was made so that the final concentration of the herbal extract prepared in Preparation Example 1 was 0.005%, 0.01%, 0.05%, 0.1% or 1%, and then the fibroblast culture medium was replaced with this medium. And incubated for 24 hours. In addition, the culture was replaced with DMEM without serum containing no herbal extract was used as a control. After incubating the cells, 10 μl of MTT solution (3- (4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide: 5 mg / ml) was added to each cultured cell. After addition and incubation for 4 hours, the medium was removed and 100 μl of dimethyl sulfoxide solution per well was added and shaken for 20 minutes.

The absorbance of the supernatant was measured at 570 nm using a microplate reader (Molecular Devices, USA). The measured value was substituted into Equation 1 below to calculate the cell proliferation effect, and the results are shown in Table 1 below.

extract(%) Absorbance Cell proliferation effect (%) Control 0.330 - 0.005 0.353 7.2 0.001 0.391 18.5 0.05 0.458 39.0 0.1 0.531 60.8 One 0.604 83.1

As can be seen in Table 1, compared with the control group not treated with the herbal extract, the treatment group treated with the herbal extract by concentration showed an effect of increasing fibroblast proliferation.

Experimental Example 2 keratinocyte proliferation effect

The proliferative effect of keratinocytes was measured in the same manner as in Experiment 1 using keratinocytes (keratinocyte, Korea Cell Line Bank), and the results are shown in Table 2 below.

extract(%) Absorbance Cell proliferation effect (%) Control 0.324 - 0.005 0.337 4.1 0.001 0.361 11.5 0.05 0.386 19.3 0.1 0.425 31.3 One 0.527 62.8

As shown in Table 2, it can be seen that keratinocyte proliferation increases as the treatment concentration of the herbal extract increases, and thus, the herbal extract of the present invention shows an excellent effect on keratinocyte proliferation.

Experimental Example 3 Collagen Synthesis Enhancement Effect

Human normal fibroblasts (Korea Cell Line Bank) were seeded in each well of a 96-well microplate to 2 × 10 ⁴ cells and incubated for 24 hours at 37 ° C. in DMEM medium. Subsequently, the herbal extract prepared in Preparation Example 1 was made of DMEM without serum to a final concentration of 0.005, 0.01 or 0.05%, and then the fibroblast culture medium was replaced with this medium and cultured for 24 hours. In addition, it was used as a control that was cultured by replacing DMEM without serum containing no herbal extract. In addition, ascorbic acid (50 g / ml) was added to promote collagen synthesis, and then incubated for another 24 hours. Each well was then washed, replaced with serum-free DMEM, and further incubated for 24 hours.

After incubation, the supernatant of each well was collected to measure the amount of newly synthesized collagen, and the amount of procollagen (procollagen) type I C-peptide (PICP) was measured using a kit (Takara, Japan). PICP amount was converted into ng / 2 × 10 ⁴ cells, and the results are shown in Table 3 below.

% Of concentration (v / v) Collagen Synthesis Amount (ng / 2 × 10 ⁴ ) Control 153 0.005 189 0.01 204 0.05 250

As can be seen in Table 3, it can be seen that the synthesis of collagen increases as the treatment concentration of the herbal extract increases, and thus the herbal extract of the present invention shows an excellent collagen synthesis promoting effect.

Preparation Example 2: Preparation of herbal composition containing food extract

A food composition containing the herbal extract of the present invention excellent in cell proliferation effect and collagen synthesis promoting effect as an active ingredient was prepared.

ingredient Content (unit mg) Ginseng concentrate powder 128.57 Angelica Extract Powder 110.00 Cheongung Extract Powder 110.00 Peony Extract Powder 142.73 Sulfur Extract Powder 84.00 Golden Extract Powder 97.50 Bokryeong Extract Powder 170.00 Cornus Extract Powder 63.75 Powder extract powder 110.00 Licorice Extract Powder 97.50 Wild ginseng root powder 0.90 Chlorella vulgaris E25 4.50 Pomegranate Extract Powder 18.00 Collagen Peptides 9.00 Aloe Aborescens 20.25 Vitamin c 4.50 Vitamin A 1.35 Sum 1172.55

Experimental Example 4: Moisturizing Effect

In order to determine whether the food composition of the present invention containing the herbal extract as an active ingredient has a moisturizing effect, the food composition of Preparation Example 2 was administered to the subject, and then water retention was measured as follows.

Thirty subjects were selected and 15 were orally administered the composition of the present invention every morning and evening. In the constant temperature and humidity room of 22 ° C and 45% RH, the moisture content of the skin was measured before oral administration, 10 days after the administration and 20 days after the administration of 30 subjects, and normal skin without any treatment was used as a control. It was. As a measuring device, a moisture content meter (corneometer CM820, Conrage + Khazaka, Germany) was used to measure the moisturizing power by measuring the electric capacity of the skin according to the moisture content of the skin. The results are shown in Table 5 below.

division Administration group Control Skin electrical conductivity before administration 50 50 Skin conductivity after 10 days of administration 87 50 Skin conductivity after 20 days of administration 95 50

As shown in the experimental results, when the composition of the present invention was administered, it was found that the electrical conductivity of the skin was higher than that of the control group not administered, and showed an excellent moisturizing effect. Therefore, it can be seen that the food composition containing the herbal extract of the present invention as an active ingredient shows an excellent effect on skin moisturizing.

Having described the specific part of the present invention in detail, it is apparent to those skilled in the art that the specific technology is merely a preferred embodiment, and the scope of the present invention is not limited thereto. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

As described above, the herbal extract of the present invention is excellent in skin cell proliferation effect and collagen synthesis enhancement effect, oral skin anti-aging cosmetic composition containing the extract as an active ingredient has excellent skin moisturizing effect to prevent skin aging phenomenon Has an excellent effect.

Claims (5)

A composition for preventing skin aging containing ginseng, cornus, medicinal herb, licorice, bokryeong, and sulfur extract as active ingredients. delete Skin moisturizing composition containing ginseng, cornus, medicinal herb, licorice, Bokryeong, and sulfuric acid extract as an active ingredient. A food composition for preventing skin aging containing ginseng, cornus, medicinal herb, licorice, bokryeong, and sulfuric acid extract as active ingredients. 5. The composition of claim 1, 3 or 4, wherein the composition comprises from 0.0001 to 10.0% by weight of each of said extracts relative to the total weight of the composition.