KR100681702B1 - Cosmetic Compositions Comprising Extract from Codonopsis pilosula Franch. Nannf. for Preventing Skin Aging - Google Patents

Abstract

The present invention relates to a cosmetic composition for preventing skin aging comprising a ginseng extract as an active ingredient, the cosmetic composition of the present invention promotes the proliferation of fibroblasts, enhance collagen synthesis, and moisturizing effect without irritation to the skin It is very useful for preventing skin aging.

Skin aging, ginseng, cosmetics, wrinkles, moisture, collagen, fibroblasts

Description

Cosmetic composition for preventing skin aging comprising a ginseng extract Cosetic Compositions Comprising Extract from Codonopsis pilosula (Franch.) Nannf. for Preventing Skin Aging}

The present invention relates to a cosmetic composition, and more particularly to a cosmetic composition for preventing skin aging comprising an extract of Codonopsis pilosula (Franch.) Nannf. As an active ingredient.

The primary function of the skin is to protect the human body from external stimuli such as temperature, humidity and ultraviolet light. However, when the skin is exposed to external stimuli such as strong ultraviolet rays, stress, and malnutrition, the skin may age, such as wrinkle formation, loss of elasticity, and keratinization. Collagen and elastic fibers (elastin) present in the dermal layer of the skin structure is the main protein that controls the elasticity of the skin.

Biosynthesis of collagen fibers is affected by internal and external factors of the skin.

Internally, natural aging (such as a decrease in estrogen) decreases cellular activity, leading to a decrease in collagen fibers.

External factors include active oxygen (free radical type) generated by excessive irradiation of UV rays and stress, which react with the -SH group of the protein to inhibit the activity of the enzyme, or decompose collagen and elastin (Matrix Metalloproteinases: MMPS) increases the expression of collagenase, causing aging that reduces wrinkles and elasticity of the skin.

In particular, over-irradiation of UV light may cause wrinkles, thickening of the skin, sagging of the skin, loss of elasticity, roughness of the skin, dryness of the skin, and skin stains that occur as skin tissue gradually changes with age. It is known to accelerate.

Currently, cosmetics or skin protectants containing various skin protection ingredients such as retinol, retinoid, vitamin C, flavonoids and tocopherol have been developed to suppress or slow down the progression of skin. In the final formulation, the potency is low, the efficacy is mild, and the skin irritation has a serious problem.

On the other hand, Korean Patent Application No. 1997-60849 discloses a cosmetic composition for preventing skin aging containing serine of retinol, alpha hydroxy acid, vitamins, especially beta hydroxy acid, and Korea Patent Application No. 1998-18636 An anti-aging cosmetic composition comprising an amino polymer as an active ingredient is disclosed. In addition, Korean Patent Application No. 1994-7002880 discloses a synergistic cosmetic composition as an antioxidant containing ginkgo extract and at least one polyphenol derivative, and Korean Patent Application No. 1996-69236 discloses L-2. A composition for preventing skin aging comprising oxothiazolidine-4-carboxylic acid derivative is disclosed.

Throughout this specification, numerous patent documents are referenced and their citations are indicated. The disclosures of the cited patents are incorporated herein by reference in their entirety, so that the level of the technical field to which the present invention belongs and the contents of the present invention are more clearly explained.

The present inventors earnestly researched to develop a cosmetic composition for preventing skin aging without side effects, and when using ginseng extract, it is possible to achieve an excellent skin anti-aging effect without the above-described side effects such as decrease in titer or skin irritation. The present invention was completed by confirming.

Accordingly, it is an object of the present invention to provide a cosmetic composition for preventing skin aging comprising a ginseng extract.

Other objects and advantages of the present invention will become apparent from the following examples and claims.

The present invention provides a cosmetic composition for preventing skin aging comprising the extract of Codonopsis pilosula (Franch.) Nannf. As an active ingredient.

The present inventors conducted a study on the native herbal medicine to improve the problems of the conventional skin anti-aging cosmetic composition showing side effects on the skin, as a result of promoting the synthesis of collagen, which is a component that gives the elasticity to the skin ginseng extract It was confirmed that collagen synthesis promotes the proliferation of fibroblasts, which are mainly composed of collagen, and enhances the skin's moisturizing power, but has almost no skin irritation side effects.

The ginseng contained as an active ingredient in the composition of the present invention is a perennial herb of ( Codonopsis pilosula (Franch.) Nannf.) Bellflower (Campanulaceae), and its root is particularly called ginseng (蔓 蔘).

In China, kodo nosis, C. pilosula (Franch.) Nannf., And kodonosis . Dried roots of C. tangshem Oliv are not called ginseng, but they are called dangsam , and the former is called Seodang and the latter is called Thangdang.

Mansam grows wild in Gangwon-do and Gyeongsangnam-do, Korea. Ginseng has been used in folk medicine to increase red blood cells and reduce white blood cells, and to enhance immunity, tonic action, and peptic ulcer caused by excessive gastric acid. In addition, ginseng is a drug that has been used as a ginseng substitute for people with side effects of ginseng (Park Jong-hee, Lee Jung-kyu, commercial medicinal plant book, p115, Shinil Trading Co., Ltd. (2000)).

On the other hand, the term "manssam extract" in the present specification means that obtained by extracting from various organs (such as leaves, flowers, stems and roots) of the ginseng, preferably means an extract obtained from the root of the ginseng.

The ginseng extract in the present invention can be obtained through various methods known in the art, preferably (a) water, (b) anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (e.g. methanol, ethanol, propanol) , Butanol, and the like), (c) acetone, (d) ethyl acetate, (e) chloroform or (f) 1,3-butylene glycol as an extraction solvent. A suitable amount of the extraction solvent is 1-20 times the dry weight of ginseng.

On the other hand, it will be apparent to those skilled in the art that the extract of the present invention can be obtained not only the above-described extraction solvent, but also the ginseng extract having substantially the same effect using other extraction solvents.

In addition, the extract of the present invention includes not only the extract by the above-described extraction solvent, but also the extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using ultrafiltration membranes having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also included in the ginseng extract of the present invention.

The ginseng extract of the present invention may be prepared in powder form by an additional process such as distillation under reduced pressure, freeze drying or spray drying.

In a preferred embodiment of the present invention, the content of the ginseng extract is 0.0001 to 10.0% by weight, more preferably 0.0005 to 10.0% by weight, most preferably 0.005 to 5.0% by weight based on the total cosmetic composition. If the content of the ginseng extract is less than 0.0001% by weight, no obvious anti-aging effect, especially wrinkle improvement, cannot be expected. If the content of the ginseng extract is more than 10.0% by weight, no obvious effect increases with the increase of the content. Formulation problems arise.

The cosmetic composition of the present invention has the use of skin anti-aging. As used herein, the term "anti-aging of skin" has the meaning encompassing comprehensively the prevention and treatment of skin aging.

The cosmetic composition of the present invention improves skin wrinkles and improves skin elasticity by promoting the proliferation of fibroblasts and the promotion of collagen synthesis, improves moisture retention and decreases the amount of water evaporation, thereby improving skin moisturizing ability. Since the cosmetic composition of the present invention improves skin wrinkles and skin elasticity and at the same time improves moisturizing power, this effect synergistically achieves the excellent anti-aging effect of the present invention. This fact is specified in the following examples.

Therefore, anti-aging of the skin, which is the use of the present invention, is interpreted to include the use of improving skin wrinkles, improving skin elasticity or improving moisture.

Ingredients included in the cosmetic composition of the present invention include ingredients commonly used in cosmetic compositions in addition to mansam extract as an active ingredient, and include, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings, and Carrier.

The anti-aging composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactants- Containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, sprays and the like, but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Hereinafter, the present invention will be described in more detail with reference to the following examples. These examples are only for illustrating the present invention in more detail, and the scope of the present invention is not limited by these examples in accordance with the gist of the present invention to those skilled in the art. Will be self-evident.

Preparation Example 1: Obtaining ginseng extract using various extraction solvents

Preparation Example 1-a

The ginseng was washed with purified water and dried, 20 g of powdered ginseng flour was added to 1.5 L of a 70% ethanol solution, and the extract was heated for 4 hours in a reflux extractor equipped with a cooling condenser. Then, the obtained extract was filtered with a 300 mesh filter cloth of Wattman filter paper, the residue was extracted once more in the same manner, and the respective extracts were combined and filtered again with filter paper of Wattman No. 5, and the solvent was removed using a vacuum concentrator. Removed and lyophilized to give 0.89 g of dry ginseng extract.

Preparation Example 1-b

The ginseng was washed with purified water and dried, and then 20 g of powdered ginseng powder was added to 1.5 l of 1,3-butylene glycol solution, and the same procedure as in Preparation Example 1-a was carried out to obtain 0.79 g of dry ginseng extract. Obtained.

Preparation Example 1-c

The ginseng was washed with purified water, dried and 20 g of powdered ginseng powder was added to 1.5 L of acetone, and the same procedure as in Preparation Example 1-a was carried out to obtain a dry ginseng extract having a dry weight of 0.65 g.

Preparation Example 1-d

The ginseng was washed with purified water and dried, and then 20 g of powdered ginseng powder was added to 1.5 L of purified water, and the same procedure as in Preparation Example 1-a was carried out to obtain a dry ginseng extract having a dry weight of 0.35 g.

Preparation Example 1-e

The ginseng was washed with purified water, dried and 20 g of powdered ginseng powder was added to 1.5 L of 70% methanol, and the same procedure as in Preparation Example 1-a was carried out to obtain a dry ginseng extract having a dry weight of 0.92 g.

Preparation Example 1-f

The ginseng was washed with purified water and dried, and then 20 g of powdered ginseng powder was added to 1.5 l of ethyl acetate solution, and the same procedure as in Preparation Example 1-a was carried out to obtain a dry ginseng extract having a dry weight of 0.65 g.

Preparation Example 1-g

The ginseng was washed with purified water and dried, and then 20 g of powdered ginseng powder was added to 1.5 l of chloroform solution, and the same procedure as in Preparation Example 1-a was carried out to obtain a dry ginseng extract having a dry weight of 0.59 g.

Preparation Example 1-h

The ginseng was washed with purified water, dried and 20 g of powdered ginseng powder was added to 1.5 l of normal butanol solution, and the same procedure as in Preparation Example 1-a was carried out to obtain 0.75 g of dry ginseng extract.

Experimental Example 1 Fibroblast Proliferation Effect

Human normal fibroblasts (Korea Cell Line Bank) are seeded to 1 x 10 ⁴ cells in each well of a 96-well microplate and incubated at 37 ° C. for 24 hours in DMEM medium (purchased by sigma). It was. Subsequently, each group of Ginseng extract prepared in Examples 1-a to 1-h was replaced with a serum-free DMEM medium containing a final concentration of 100 μg / ml and a serum-free DMEM medium containing no ginseng extract Controls replaced with were further incubated for 24 hours. Then, 10 μl of MTT solution (3- (4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide: 5 mg / ml) was added to each, and incubated for 4 hours. The medium was then removed, shaken for 20 minutes by addition of 100 μl of dimethyl sulfoxide solution per well, and the absorbance of the supernatant was measured at 570 nm using a microplate reader (Molecular Devices, USA). . The measured values were substituted into the following Equation 1 to calculate the cell proliferation effect, and the results are shown in Table 1 below.

Cell proliferation effect (%) = {(absorbance at extract treatment-absorbance of control) / (absorbance of control)} x 100

extract Absorbance Cell proliferation effect (%) Control 0.355 - Preparation Example 1-a 0.499 40.6 Preparation Example 1-b 0.455 28.2 Preparation Example 1-c 0.477 34.4 Preparation Example 1-d 0.431 21.4 Preparation Example 1-e 0.475 33.8 Preparation Example 1-f 0.445 25.4 Preparation Example 1-g 0.489 37.7 Preparation Example 1-h 0.440 24.0

As can be seen in Table 1, it can be seen that the fibroblast proliferation effect of about 20-40% at 100 ㎍ / ㎖ concentration of the ginseng extract compared to the control group not treated.

Experimental Example 2 Cell Proliferation Effect According to the Concentration of Manju Extract

Except for using 50, 100 or 200 ㎍ / ㎖ Mansi extract prepared in Preparation Example 1-a is confirmed to have the best fibroblast proliferation effect in Experimental Example 1, the cells in the same manner as Experimental Example 1 The proliferative effect was measured and the results are shown in Table 2 below.

Concentration (μg / ml) Absorbance (570 nm) Cell proliferation effect (%) Control 0.325 - 50 0.424 30.46 100 0.465 43.08 200 0.510 56.92

As can be seen in Table 2, it can be seen that the ginseng extract promotes the growth of fibroblasts in a concentration-dependent manner, and also the results in Experimental Example 1 are significant.

Experimental Example 3: Collagen Synthesis Enhancement Effect

Human normal fibroblasts (Korea Cell Line Bank) were seeded in each well of a 96-well microplate to be 2 × 10 ⁴ cells and incubated for 24 hours at 37 ° C. in DMEM medium. Subsequently, the experimental group in which the ginseng extract prepared in Example 1-a was replaced with a serum-free DMEM medium containing a final concentration of 50, 100 or 200 μg / ml and a serum-free DMEM medium containing no ginseng extract were replaced. One control was further incubated for 24 hours, ascorbic acid (50 g / ml) was added to promote collagen synthesis, followed by another 24 hours. Each well was then washed and replaced with serum-free DMEM medium and incubated for an additional 24 hours. After incubation, the supernatant of each well was collected and the amount of newly synthesized collagen was measured using a kit (Takara, Japan) for the amount of procollagen (procollagen) type I C-peptide (PICP). PICP amount was converted to ng / 2 × 10 ⁴ cells, and the results are shown in Table 3 below.

Concentration (μg / ml) Collagen Synthesis (ng / 2 x 10 ⁴ ) Control 157 50 193 100 213 200 243

As can be seen in Table 3, it can be seen that the synthesis of collagen increases as the concentration of the ginseng extract increases, and thus, the ginseng extract exhibits an excellent collagen synthesis enhancing effect.

Examples and Comparative Examples

To the flexible cosmetics cosmetic composition having a composition ratio of Table 4 to prepare an example containing 1.0% by weight of the ginseng extract prepared in Preparation Example 1-a and a comparative example does not contain the ginseng extract, used in Experimental Example 4 below Was:

division Example Comparative Example ingredient Content (% by weight) Content (% by weight) Ginseng extract 1.0 - glycerin 5.1 5.1 Propylene glycol 4.2 4.2 Tocopheryl Acetate 3.0 3.0 Liquid paraffin 4.6 4.6 Triethanolamine 1.0 1.0 Squalane 3.1 3.1 Macadamia Nut Oil 2.5 2.5 Polysorbate 60 1.6 1.6 Sorbitan sesquirrolate 1.6 1.6 Propylparaben 0.6 0.6 Carboxyl Vinyl Polymer 1.5 1.5 incense a very small amount a very small amount antiseptic a very small amount a very small amount Purified water Remaining amount Remaining amount system 100 100

Experimental Example 4: Moisturizing Effect

In order to evaluate the moisturizing power of each cosmetic prepared in the above Examples and Comparative Examples, the moisture retention capacity and the water evaporation amount of the skin to which each cosmetic was applied were measured as follows:

Experimental Example 4-1: Measurement of Moisture Retention Capacity

In the constant temperature and humidity room of 22 ° C. and 45% RH, each of the cosmetics prepared in Examples and Comparative Examples was coated with a predetermined amount (0.03 g / 16 cm 2) in the inner half of 30 test subjects, before application and after 1 hour of application. And moisture content of the skin 2 hours after application was measured, and normal skin without any treatment was used as a control. As a measuring device, a moisture content meter (corneometer CM820, Conrage + Khazaka, Germany) was used to measure the moisturizing power by measuring the electric capacity of the skin according to the moisture content of the skin. The results are shown in Table 5 below.

division Example Comparative Example Control Skin electrical conductivity before application 50 50 50 Skin electrical conductivity 1 hour after application 85 77 50 Skin electrical conductivity 2 hours after application 75 61 50

Experimental Example 4-2: Measurement of Water Evaporation Amount

After tape stripping on the upper arm of the arm to weaken the skin's protective film, apply the cosmetics to the subject in the same manner as in Experiment 4-1, and then use a moisture evaporator (TEWA meter, koln, Germany). Federated water evaporation was measured 15 times in 1 hour, 2 hours, 4 hours, 6 hours, and 8 hours, and the average value was obtained. The results are shown in Table 6 below.

division Example Comparative Example Control Moisture evaporation after 0 hours 4.0% 4.0% 0.0% Moisture evaporation after 1 hour -8.1% -4.5% -0.3% Water evaporation after 2 hours -7.6% -0.9% 0.0% Water evaporation after 4 hours -4.9%  2.1% 0.1% Water evaporation after 6 hours -2.1%  2.9% -0.2% Water evaporation after 8 hours -1.5% 3.1% 0.0%

As shown in the results of Experimental Examples 4-1 and 4-2, the cosmetics of the examples of the present invention had higher electrical conductivity of the skin and less water evaporation amount than the cosmetics of the comparative examples. Therefore, it can be seen that the cosmetic of the present invention exhibits an excellent moisturizing effect.

Experimental Example 5: Skin irritation test

In order to determine the skin irritation of each ginseng extract prepared according to the preparation example, a predetermined amount (about 0.1 g) of each sample at 5 x 20 cm in the lower part of both arms for 30 men and women healthy adults for 24 hours After patching for 1 hour and 24 hours after the removal, the skin condition change was visually read according to the following criteria, and the stimulation rate was calculated according to the following equation. The experimental results are shown in Table 7 below.

Criteria

-: No erythema or unusual phenomenon

+-: Slightly reddish

+: Significantly redder than the surroundings

++: reddens and swells more heavily

Stimulus rate = {((+-) number x1 + (+) number x2 + (++) number x3) / (total number x3)} x100

Production Example Judgment result Stimulation rate (%) ++ + +- - 1-a - - 2 28 2.2 1-b - - One 29 1.1 1-c - - 2 28 2.2 1-d - - One 29 1.1 1-e - - One 29 1.1 1-f - - 3 27 3.3 1-g - - 2 28 2.2 1-h - - One 29 1.1

As can be seen from the skin irritation test results of Table 7, the ginseng extract of the present invention can be seen that there is no skin irritation.

Formulation Example 1

Formulation examples of the flexible lotion (skin) in the cosmetic containing the ginseng extract obtained in Example 1-a is as follows:

ingredient Content (% by weight) Ginseng extract 1.0 1,3-butylene glycol 5.2 Oleyl alcohol 1.5 ethanol 3.2 Polysorbate 20 3.2 Benzophenone-9 2.0 Carboxyl Vinyl Polymer 1.0 glycerin 3.5 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 2

Formulation example of the milk lotion in the cosmetic composition containing the ginseng extract obtained in Example 1-a is as follows:

ingredient Content (% by weight) Ginseng extract 1.0 glycerin 5.1 Propylene glycol 4.2 Tocopheryl Acetate 3.0 Liquid paraffin 4.6 Triethanolamine 1.0 Squalane 3.1 Macadamia Nut Oil 2.5 Polysorbate 60 1.6 Sorbitan sesquirrolate 1.6 Propylparaben 0.6 Carboxyl Vinyl Polymer 1.5 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 3

An example of the formulation of the nourishing cream in the cosmetic composition containing the ginseng extract obtained in Example 1-a is as follows:

ingredient Content (% by weight) Ginseng extract 2.5 glycerin 4.0 vaseline 3.5 Triethanolamine 2.1 Liquid paraffin 5.3 Squalane 3.0 Beeswax 2.6 Tocopheryl Acetate 5.4 Polysorbate 60 3.2 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 3.1 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 4

An example of the formulation of the massage cream in the cosmetic containing the ginseng extract of the present invention is as follows:

ingredient Content (% by weight) Ginseng extract 0.5 glycerin 4.0 vaseline 3.5 Triethanolamine 0.5 Liquid paraffin 24.0 Squalane 3.0 Beeswax 2.1 Tocopheryl Acetate 0.1 Polysorbate 60 2.4 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 2.3 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 5

Formulation examples of the pack in the cosmetic containing the ginseng extract obtained in Example 1-a is as follows:

ingredient Content (% by weight) Ginseng extract 1.5 Ethyl alcohol 3.0 EDTA-2Na 0.02 Propylene glycol 5.1 Glyceline 4.5 Cabopol 1.0 Polyoxide 0.1 antiseptic a very small amount incense a very small amount Purified water Remaining amount system 100

As described in detail above, the present invention provides a cosmetic composition for preventing skin aging. As the cosmetic composition of the present invention further comprises a ginseng extract in the conventional cosmetics, it promotes the proliferation of fibroblasts and collagen synthesis, improves the skin's moisturizing ability, and is very effective in preventing skin aging, and irritation to the skin. Because of this very small, it is very useful as a cosmetic composition for preventing skin aging without side effects on the skin.

Claims (4)

Cosmetic composition for preventing skin aging comprising the extract as an active ingredient. The cosmetic composition for preventing skin aging according to claim 1, wherein the amount of the ginseng extract is 0.0001-10.0 wt% based on the total cosmetic composition. The method of claim 1, wherein the ginseng extract is (a) water, (b) anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, (c) acetone, (d) ethyl acetate, (e) chloroform or (f) 1, A cosmetic composition for preventing skin aging, which is obtained by using 3-butylene glycol as an extraction solvent. The composition of claim 1 wherein the cosmetic composition consists of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Cosmetic composition for preventing skin aging, characterized in that it has a formulation selected from the group.