KR20090055079A - The cosmetic composition for prevention of the skin aging containing the dioscorea japonica thunb extracts - Google Patents

Abstract

A cosmetic composition for preventing skin aging, which contains a Dioscorea japonica Thunb extract is provided to proliferate skin cell and promote the biosynthesis of collagen and DHEA(dehydroepiandrosterone). A cosmetic composition for preventing skin aging comprises 0.001-30.0 weight% Dioscorea japonica Thunb extract as an active ingredient. A solvent for extracting the Dioscorea japonica Thunb is one or more selected from purified water, anhydrous alcohol of 1-4 carbon atoms, acetone, ethylacetate, chloroform, glycerin, butylenes glycol, propylene glycol, dichloromethane, hexane, and benzene. The composition is used in a form of skin, lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, spray or powder.

Description

The cosmetic composition for prevention of the skin aging containing the Dioscorea japonica Thunb extracts}

The present invention relates to a cosmetic composition containing the wild yam extract as an active ingredient, more specifically, the wild yam extract is keratinocyte proliferation effect, collagen biosynthesis promoting effect, adrenal cortex hormone dehydroepiandrosterone (dehydroepiandrosterone: By promoting the biosynthesis of DHEA), the present invention relates to a cosmetic composition for preventing skin aging, which is excellent in moisturizing skin and improving skin wrinkles.

Aging is a process of gradual deterioration of the body's structure and function, a rapid increase in susceptibility to disease and death, and a physiological change in the body, resulting in a significant deterioration of the entire body. . Among them, we can easily feel that skin aging is as important as diabetes, heart disease, arteriosclerosis, dementia, cancer, etc. Although there are individual differences, skin aging usually begins around the early 20s and ages. Appears to increase as the number increases.

Skin aging is influenced by various factors such as genetic and environmental, which can be divided into two types. One is endogenous aging, which is natural aging, and it is aging that occurs over time according to genetic information. The other is called aging due to extrinsic aging. It refers to the aging phenomenon caused by ultraviolet rays present in the. Endogenous aging is mainly observed in skin that is not exposed to sunlight, characterized by wrinkles and reduced skin elasticity, and photoaging occurs in areas exposed to sunlight, deep wrinkles, rough skin, pigmentation, It is characterized by a decrease in skin tone and skin elasticity.

Scholars who study aging phenomena call the 21st century "the age of hormones." Proper use of hormones is expected to open the way to prevent aging. Indeed, research efforts to use hormones to prevent or reverse aging are currently underway around the world and are already making significant progress. Accordingly, much progress is being made in the study of the role and function of the hormone causing aging. Of course, the scholars also acknowledge that hormones are not the only factor that causes aging, and that genetic and environmental factors have a profound effect on aging.

In women in their 40s, estrogen secretion decreases skin wrinkles and increases skin dryness, while hormonal decline leads to aging due to aging of body organs and reduced metabolism. Recently, the most attention is DHEA known as corticosteroids synthesized in the adrenal cortex. About 80 kinds of hormones are synthesized in human organs, and DHEA is the most related to sex hormones. DHEA is a hormone that is produced after cholesterol is converted to pregnelonon and is a precursor to sex hormones testosterone and estrogen. DHEA, along with dehydroepiandrosterone-sulfate (DHEA-S), is the most abundant hormone in the human body. If the biosynthesis is made smoothly it will be possible to suppress the progress of skin aging.

Therefore, an object of the present invention is to provide a cosmetic composition excellent in the skin aging prevention effect.

Accordingly, the present inventors have conducted research on active ingredients that improve skin condition by preventing skin aging, and found that wild yams contain diosgenin, a precursor of DHEA, and utilize wild yams. By preparing an extract and a cosmetic composition containing the same, the wild yam extract promotes collagen biosynthesis, skin cell proliferation, DHEA biosynthesis, skin moisturization, and skin wrinkle improvement, thereby improving skin condition.

The present invention is easy to prevent skin aging due to the skin cell proliferation effect of wild yam extract, collagen biosynthesis promoting effect, promoting the biosynthesis of dehydroepiandrosterone (DHEA), adrenal cortex hormone, skin moisturizing and wrinkle improvement Can be used.

The anti-aging cosmetic composition containing the wild yam extract according to the present invention is characterized in that the wild yam extract is contained in an amount of 0.001 to 30.0% by weight based on the total weight of the composition.

The wild yam used in the present invention is the root of the yam ( Dioscorea japonica Thunb ), which is native to Korea and is distributed in mountainous regions of the country except the mountainous region where the climate is very cold, and is a vine perennial herbaceous plant. . The leaves are subtle but sometimes regenerated, with long lobes, 5-10 cm long, 2-2.5 cm wide, long oval or narrow triangles, with pointed ends. Flowers are hermaphrodite, bloom in June-July, stems are wound on objects, many branches are split, and roots are long cylindrical. In oriental medicine, yam has been used for the treatment of frostbite, polyuria, urinary incontinence, skin eczema, etc., and is recently used for the treatment of diabetes mellitus, and has been used for nutrition, tonic, and bosin. The active ingredient contained in the yam is known to contain diosgenin, vitamin C, polyphenoloxidase, glycoprotein, saponin, and the like.

Wild yam extract included in the cosmetic composition of the present invention can be obtained by various extraction methods, specifically, as an extraction solvent, purified water, anhydrous alcohols having 1 to 4 carbon atoms (methanol, ethanol, propanol, butanol), acetone, ethyl It is a solvent extraction method using at least one solvent selected from acetate, chloroform, glycerin, butylene glycol, propylene glycol, dichloromethane, hexane, benzene, etc. by mixing two or three kinds of solvents. The ratio of the wild yam grinds and the solvent is 1: 5 to 1:30, preferably 1:20. Extraction is performed by immersion for 7-15 days while maintaining a temperature of 15-45 ° C. in the extractor, or extraction at 40-100 ° C. for 5-20 hours, and concentrated under reduced pressure or lyophilization.

However, the extraction solvent used in the preparation of the wild yam extract of the present invention is not limited to the above-mentioned extraction solvent, it can be obtained that wild yam extract having substantially the same effect using other extraction solvent can be obtained. It is self-evident to.

On the other hand, the wild yam extract is characterized in that it contains 0.001 to 30.0% by weight, preferably 0.1 to 10.0% by weight relative to the total weight of the cosmetic composition. When the content of the wild yam extract is less than 0.001% by weight it is difficult to expect the effect, when it exceeds 30.0% by weight does not appear to increase the apparent effect of increasing the content.

The active ingredient included in the cosmetic composition of the present invention is a wild yam extract and includes other ingredients commonly used in cosmetic compositions, such as conventional containing agents such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings. And the like.

Anti-aging cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing It may be formulated as such, but is not limited thereto.

When the formulation of the present invention is a paste, cream or gel, the carrier components include animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide and the like. Can be used.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol , Fatty acid esters of 1,3-butylene glycol, oils, glycerol aliphatic esters, polyethylene glycols or sorbitan.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the dosage form of the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester as a carrier component , Microcrystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The cosmetic composition of the present invention has an anti-aging effect. This is to improve skin aging through the DHEA synthesis promoting effect, epidermal cell regeneration effect, collagen biosynthesis promoting effect of wild yam extract. Accordingly, the present invention is construed to include anti-aging, wrinkle improvement, and skin elasticity use.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, and the scope of the present invention is not limited to these examples according to the gist of the present invention to those skilled in the art to which the present invention pertains. Will be self explanatory.

Production Example  1: wild yam  Preparation of Extract

500 g of wild yam purchased commercially is washed with clean water and dried in a cool shade. Thereafter, finely crushed wild yam using a grinder, 250g of the pulverized product is placed in an extractor equipped with a cooling condenser, and 5 L of 80% ethanol is added and extracted by heating at 75 ° C. for 15 hours. The extract was filtered with a 400 mesh filter cloth, and the filtrate was collected and concentrated under reduced pressure at 55 to 60 ° C. in a distillation apparatus equipped with a cooling condenser and dried under vacuum to obtain 25.4 g of an extract powder.

Experimental Example  One : Wild yam  Collagen Production Effect of Extracts

Collagen production effect was confirmed using the crystal violet method. This method is an experiment to determine the ability of protein synthesis, crystal violet dye solution is a reagent that binds to the protein, utilizing this feature to measure collagen synthesis capacity.

Fibroblasts (Human fibroblast) were dispensed in each well of a 96-well plate (5 x 10 ³ cells) and incubated for 24 hours in a medium containing 10% fetal calf serum. After changing to a medium without fetal calf serum, the wild yam extract prepared in Preparation Example 1 was treated with 0.005, 0.05, 0.5% of cells and incubated for 48 hours. After 48 hours, the culture solution was removed, washed once with PBS, and then dried at 37 ° C. for 30 minutes. Thereafter, crystal violet reagent was added and stained at room temperature for 5 minutes, and the dye solution was washed well with distilled water. The combined dye was dissolved by adding 0.2 M phosphoric acid and ethanol, and after 5 minutes, ELISA Reader (Molecular Devices, USA) Absorbance was measured at 590 nm. The control group of the experiment was used as a non-treated wild yam extract, the growth capacity was obtained by comparison with the control group. The results are shown in Table 1 below.

sample Collagen Production Capacity (%) Control 100.0 0.005% 117.4 0.05% 134.1 0.5% 178.5

As can be seen from Table 1, the wild yam extract has a collagen synthesis promoting effect, and in particular, the effect is increased in a concentration-dependent manner.

Experimental Example  2: keratinocyte proliferation effect test

The wild yam extract prepared in Preparation Example 1 was carried out the following experiment to determine the cell growth promoting effect.

The keratinocytes cultured in DMEM (Dulbecco's Modified Eagle's Media) containing 10% fetal calf serum are aliquoted to 5 × 10 ³ cells / well in a 96-well plate incubator and replaced with medium containing no fetal calf serum. Then incubated for one day. Thereafter, the wild yam extract of Preparation Example 1 was treated to cells at 0.005, 0.05, and 0.5%, followed by incubation for 48 hours, and 10 μl of MTT (methylthiazolydiphenyl-tetrazolium bromide, sigma) (5mg / ml) was added to the cells. After incubation for 4 hours, the medium was removed, 100 μl of DMSO (dimethyl sulfoxide) was added to each well, and the absorbance was measured at 570 nm with an ELISA Reader (Molecular Devices, USA).

% Cell proliferation = (absorbance of test group-absorbance of control group) / absorbance of control group x 100

Cell growth promoting effect sample Keratinocyte proliferation effect (%) Control 0 0.005% 24 0.05% 48 0.5% 79

As can be seen from Table 2, the wild yam extract had a cell proliferation effect and had a cell proliferation effect in a concentration-dependent manner.

Production Example  2

Prepared in the form of a cream as a composition ratio of the following Table 3, but prepared in Example 1 and 2 and the comparative composition does not contain the wild yam extract of Preparation Example 1 and 2.5% and 5%.

ingredient Example 1 (% by weight) Example 2 (% by weight) Comparative Example 1 (% by weight) Preparation Example 1 5.0 2.5 Beeswax 1.0 1.0 1.0 Polysorbate 60 0.5 0.5 0.5 Liquid paraffin 10.0 10.0 10.0 Squalane 3.0 3.0 3.0 Sorbitan stearate 1.0 1.0 1.0 Glyceryl Stearate 1.5 1.5 1.5 Stearic acid 1.5 1.5 1.5 glycerin 5.0 5.0 5.0 Propylene glycol 5.0 5.0 5.0 Caroxyvinyl Polymer 0.1 0.1 0.1 Triethanolamine 0.1 0.1 0.1 antiseptic a very small amount a very small amount a very small amount Purified water Remaining amount Remaining amount Remaining amount system 100 100 100

Experimental Example  3: Skin Patch Test

In order to confirm the skin safety of the cosmetic composition containing the wild yam extract of the present invention, there has never been a hypersensitivity reaction to skin irritation in the past, and the skin of 20 females in their 20s and 30s with no skin disease or skin allergy symptoms A patch test was done. The test site was first wiped with 70% ethanol and then dried. The materials of Examples 1 and 2 and Comparative Example 1 were added dropwise into the Fin chamber (Finn chamber, 100x10, EPITEST, Finland) by 15 µg, and then sealed in the forearm inner part of the test subject. The patch was patched for 24 hours, the patch was removed, and the test site was marked with a pen. After 1 hour and 24 hours after marking, the test site was observed using a magnifying glass (8MC-150, DAZOR, United States of America) to observe erythema and edema. The skin response was determined according to the regulations of the International Contact Dermaitis Research Group (ICDRG), and the average skin response rate was calculated according to the following equation, and the results are shown in Table 4.

* Evaluation criteria and score of skin reaction

Symbol score Evaluation standard - 0 No response ± 0.5 Faint or light erythema  +  One Boundary but weak erythema, edema and papules  ++ 2 Pronounced erythema, papules and vesicles  +++ 3 Severe erythema and alveolar, scleroderma

Average skin reactivity = (score x responses x 100 x 1/2) / [3 (maximum score) x total number of subjects (n)]

Test substance After 1 hour After 24 hours Reactivity (%) (n = 20)  ± + ++ ± + ++ Example 1 - - - - - - 0.00 Example 2 - - - - - - 0.00 Comparative Example 1 - - - - - - 0.00

As can be seen from Table 4, there was no skin irritation in the cosmetics containing the wild yam extract, so skin safety was excellent.

Experimental Example  4 : DHEA To promote biosynthesis

To evaluate the DHEA biosynthesis-promoting effect of wild yam extract, 10 healthy women in their 40s were divided into three groups of 10 people. After applying the materials of Examples 1 and 2 and Comparative Example 1 twice each morning and evening for 4 months, the amount of increase in DHEA was measured. To measure DHEA before and after applying the cream, the subject's saliva was obtained using Salivat (Salivette: Salstedt, Germany), and radioimmunoassay (Diagnostic Immunoassay: Diagnostic Products) to measure the amount of DHEA in saliva. Corporation, USA). The results are shown in Table 5 below, and the results are average values.

Composition DHEA (nmol / ℓ) 4 months ago 4 months later Example 1 0.64 0.88 Example 2 0.63 0.79 Comparative Example 1 0.63 0.65

As can be seen from Table 5, the cosmetics containing wild yam extract promoted the biosynthesis of DHEA, and the higher the content of wild yam extract, the higher the biosynthesis of DHEA.

Experimental Example  5

In order to measure the effect of the skin moisturizing power of Examples 1, 2 and Comparative Example 1 prepared in Preparation Example 2, three of 60 healthy workplace women in their 30s to 40 in one group It was divided into groups and measured. Moisturizing power was measured after application to the right side and the left side of each face twice daily for four weeks, morning and evening. Moisturizing power was measured using a corneometer (CM825, Courage + Khazaka GmbH, Germany), the measurement was carried out at constant temperature, constant humidity (25 ℃, 40% humidity). The experimental results comparing the application before and after application are shown in Table 6 below, and the value is an average value.

Experiment period Moisturizing power improvement (%) Example 1 Example 2 Comparative Example 1 Week 0 1 week 14 8 5 2 weeks 25 17 7 3 weeks 38 24 8 4 Weeks 44 33 13

As can be seen from Table 6, the cosmetics containing the wild yam extract increased the skin moisturizing power, and the higher the content, the more the cosmetics were increased.

Experimental Example  6: skin wrinkle improvement

After applying the cosmetic composition containing the wild yam extract of the present invention to the human body, the degree of skin wrinkle improvement was evaluated. Sixty healthy women in their 30s and 40s were divided into three groups of 20, Example 1 and 2 prepared in Preparation Example 2, and applied twice a day for 2 months. After that, the replica was made using silicone, and the wrinkles were analyzed by visiometer (SV600, Courage + Khazaka electronic GmbH, Germany) for image analysis. The results show the average of the values after 8 weeks of application and the values before application for 20 people, and the results are shown in Table 7 below.

Cosmetics R1 R2 R3 R4 R5 Example 1 -0.26 -0.25 -0.21 -0.05 -0.04 Example 2 -0.23 -0.22 -0.17 -0.03 -0.03 Comparative Example 1 -0.19 -0.20 -0.12 -0.05 -0.04

R1: difference between the highest and lowest of the wrinkle contour

R2: Average of R1 values after arbitrarily dividing the wrinkle contour by 5 spaces

R3: Highest value of R1 divided by 5

R4: Mean value of base line of wrinkled contour minus the value of the top and valley of each angle

R5: Mean value of the base line of the wrinkle contour, minus the wrinkle lines of each wrinkle

As can be seen from Table 7, Examples 1 and 2 containing wild yam extract showed an improvement in skin wrinkles compared to Comparative Example 1.

Hereinafter, examples of the cosmetic formulation containing the wild yam extract of the present invention include a flexible lotion, an essence, a pack, a shampoo and a body cleanser, but the formulation of the cosmetic of the present invention is not necessarily limited thereto.

Formulation example  1: skin lotion

Skin lotion containing the wild yam extract prepared in Preparation Example 1 was prepared according to the conventional method in the composition of Table 8.

Raw material name Content (% by weight) Preparation Example 1 5.0 1,3-butylene glycol 5.2 Oleyl alcohol 1.5 ethanol 3.2 Polysorbate 20 3.2 Benzophenone-9 2.0 Carboxyl Vinyl Polymer 1.0 glycerin 3.5 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation example  2: essence

Essence containing the wild yam extract prepared in Preparation Example 1 was prepared according to the conventional method in the composition of Table 9.

Raw material name Content (% by weight) Preparation Example 1 5.0 1,3-butylene glycol 4.0 glycerin 3.0 Allantoin 0.5 Panthenol 0.1 EDTA-2Na 0.01 ethanol 5.0 Triethanolamine 1.5 Squalane 2.0 Beeswax 2.5 Polysorbate 60 3.5 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 2.5 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation example  3: pack

A pack containing the wild yam extract prepared in Preparation Example 1 was prepared according to a conventional method with the composition of Table 10.

Raw material name Content (% by weight) Preparation Example 1 5.0 Ethyl alcohol 3.0 EDTA-2Na 0.05 Propylene glycol 5.0 Glyceline 4.5 Cabopol 1.5 Polyoxide 0.2 antiseptic a very small amount incense a very small amount Purified water Remaining amount system 100

Formulation example  4: shampoo

Shampoo containing wild yam extract prepared in Preparation Example 1 was prepared according to a conventional method with the composition of Table 11.

Raw material name Content (% by weight) Preparation Example 1 5.0 Alkyl sulfate (30%) 25.0 Alkyl ether sulfate (30%) 20.0 Alkylamidobetaine (30%) 5.0 Propylene glycol 3.0 Lauramid 4.0 EDTA-2Na 0.1 Polyquaternium-10 0.05 NaCl 0.1 Citric acid 2.4 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation example  5: body Cleanser

A body cleanser containing the wild yam extract prepared in Preparation Example 1 was prepared according to a conventional method with the composition of Table 12.

Raw material name Content (% by weight) Preparation Example 1 5.0 Alkyl ether saccharide (30%) 30.0 Alkyl ether sulfosuccinate (30%) 20.0 Alkylamidobetaine (30%) 5.0 Propylene glycol 5.0 Betaine 5.0 Lauramiddiei 4.0 Cellulose gum 0.05 Hydrolyzed protein 0.1 Citric acid a very small amount NaCl a very small amount incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Claims (5)

Cosmetic composition for preventing skin aging, comprising a wild yam extract as an active ingredient. The cosmetic composition for preventing skin aging according to claim 1, wherein the wild yam extract is contained in an amount of 0.001 to 30.0 wt% based on the total weight of the composition. The cosmetic composition for preventing skin aging according to claim 1, wherein the wild yam extract exhibits DHEA biosynthesis promotion, cell growth promotion, collagen synthesis promotion, or a combination thereof. The method of claim 1, wherein the wild yam extract is at least one selected from the group consisting of purified water, anhydrous alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, chloroform, glycerin, butylene glycol, propylene glycol, dichloromethane, hexane, benzene Extracted using a solvent, the solvent is a skin composition for preventing skin aging, characterized in that the amount is added in an amount of 5 to 30 times the weight of the wild yam. The group according to any one of claims 1 to 4, wherein the composition is composed of softener, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder. Cosmetic composition for preventing skin aging, characterized in that it has a formulation selected from.