KR101463842B1 - Skin external composition for recovering inflammation or hurt containing extracts of herbal medicine - Google Patents

Abstract

The present invention relates to a herbal medicine comprising at least one herbal medicine extract, selected from the group consisting of mulberry, coriander, health, manor or gold; One or more of the extracts of the crude extracts, the herbal health extracts, the extracts of the perennial extracts or the extracts of the runners extracted from the herb extracts after the herb treatment; Or a mixture of the herbal medicine extract and a feces extract as an active ingredient, and a composition for wound healing. The composition according to the present invention provides wound healing efficacy by inhibiting inflammation-related factors and promoting re-epithelialization of wound tissues.

Fojyeon, Songjeol, Corn oil, Health, Ganoderma, Golden, Core, Cleanser, Runner, Oriental herb medicine, Processing, Wound healing, Anti inflammation, External medicine

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a skin external composition for skin irritation or wound healing,

The present invention relates to herbal extracts of jade, corn oil, health, manor or gold; Herbal extracts, herbal health extracts, perennial extracts or prickly golden extracts; Or an extract of herbal medicine and a feces extract as an active ingredient, and a composition for wound healing.

The skin acts as a shield to protect the body from the outside. When a wound occurs in the skin, the wound is filled with blood by the natural healing action of the body, and the granule of the platelet is reduced, and the activation of the hageman factor starts and the wound healing process proceeds. Blood coagulation is a temporary defensive action that protects exposed wound tissues and provides the basis for the migration of cells during the healing process (Lee, SH, Jung SG, Skin Barrier, Seoul: Ryo Moon Gak, 2004).

The wound healing process is divided into four stages: inflammation, reepithelialization, proliferation and maturation. During inflammation, immune cells appear in the wounds, and these cells migrate from the blood vessels to the wound site. Followed by growth factors and signaling molecules that induce granule tissue formation. In the absence of significant infection, the inflammatory period is generally short (Care KRGf.Advances in wound care. Seoul: Korea Medical Book Publisher, 2002). Inflammation is a necessary step in the wound healing process.

Proliferation is similar to re-epithelialization, and granular tissue is formed at the wound site (Kubo KKY, Spongy matrix of hyaluronic acid and collagen as a cultured dermal substitute: evaluation in an animal test) J Artif Organs 2003; 6 (1 ): 64-70). The granular tissues are composed of a combination of extracellular matrix components such as immature collagen, fibronectin and hyaluronic acid along with fibroblasts and inflammatory cells, It is important for tissues to heal wounds quickly by filling in the wound and organizing it. A new epidermis is created and the epithelium is reconstructed as the surface of the wound is covered by a layer of keratinocytes. Cells migrate through the wound from the wound edge or the dermis remnants of the remaining skin and begin migrating through the connective tissue under the scab.

When the re-epithelialization of the wound is completed, a series of processes where the wound area is reduced through the increase of the connective tissue and the reorganization proceed. Subsequently, during maturation, the junk cells and capillaries of the convalescent tissue gradually disappear, causing scarring if these tissues are hyperplasia or not normally degraded. This is a common wound healing process. In wound healing, it is important not only to treat wounds quickly but also to treat them without side effects or scars. Therefore, it is more important that the tissue cells are balanced through the suppression of inflammation and the regulation of the expression of growth factors. Efforts are underway.

Reduction of inflammation can be measured by decreasing the density of inflammatory cell types, such as monocytes or astrocytes, neutrophils, mast cells, and basophils. The reduction of inflammation can also be measured by measuring neutrophil activity (Jones et al., 1994). Factors such as frequency of mast cell degranulation or measurement of histamine levels or levels of reactive oxygen species can also be used as a measure of inflammation reduction. Levels of inflammation may also be determined by PCR using specific genes such as interferon-alpha, -beta and -gamma, tumor necrosis factor-alpha, interleukin 1 beta, -2, -4, -5, -6, -8, -18, -23, -27, CD4, CD28, CD80, CD86, MHCII, and iNOS. Measurement of tissue and / or patient fluid, such as plasma proinflammatory cytokines, may be a measure of inflammation reduction.

Pine tree(Pinus densiflora) In the case of a knot and a branching part, the part where the pine branch grows or the part where the pine branch is cut is included. The part where the pine branch grows is the place where the pine root is gathered. In the case of the cut branch, the pine root is gathered and its part turns to light yellow. Songjeol has been used for a long time as a custom, arthritis, and is said to be able to cure weak legs when it is drunk (JP Patent Publication No. 2004-0094028, "Method of manufacturing wine using transsection") . This suggests that various active ingredients are contained in gypsum.

Golden ( Scutellaria baikalensis ) has been shown to be effective against skin cells (JP 2007-0036224, " Composition containing cosmetic herbal extracts "), antioxidant (Gabrielska J, Oszmianski J, Zyłka R, Komorowska M. Antioxidant activity of flavus from Scutellaria baicalensis in (Yang D, Michel D, Bevalot F, Chaumont JP, Millet-Clerc J. [Antifungal activity in vitro of Scutellaria baicalensis Georgi (Tokushima et al., 1995), anti-cancer effects (Konoshima T, Kokumai M, Kozuka M, Iinuma M, Mizuno M, Tanaka T, Tokuda H, Nishino H, 40 (2): < RTI ID = 0.0 > (2): < / RTI > 531-3.) And the like. This suggests that gold contains various active ingredients.

Ganoderma japonicum , G. lucidum ) have been shown to be anti-aging (Chen K, Li C. Recent advances in studies on traditional Chinese anti-aging materia medica. J Tradit Chin Med 1993 Sep 13 (3): 223-6, , Antioxidant activity (Lin JM, Lin CC, Chen MF, Ujiie T, Takada A. Radical scavenger and antihepatotoxic activity of Ganoderma formosanum, Ganoderma lucidum and Ganoderma neo- japonicum J Ethnopharmacol 1995 Jun 23; 47 (1): 33-41 ), Anti-inflammatory, anticancer efficacy (Akihisa T, Nakamura Y, Tagata M, Tokuda H, Yasukawa K, Uchiyama E, Suzuki T. Kimura Y. Anti- inflammatory and anti-tumor-promoting effects of triterpene acids and sterols from the fungus Ganoderma lucidum. Chem Biodivers. 2007 Feb; 4 (2): 224-31).

Cornus officinalis) has been reported to have a whitening effect (J Toxicol Environ Health A. 2007 Mar 1; 70 (5):.. 393-407 Inhibitory effects of plant extracts on tyrosinase, L-DOPA oxidation, and melanin synthesis Hwang JH, Lee BM.).

Health ( Zingiber officinale) is an anti-inflammatory effect (Planta Med 2007 Dec; 73 ( 15.):. 1525-30 Epub 2007 Dec 3. Evaluation of the topical anti-inflammatory activity of ginger dry extracts from solutions and plasters), anti-wrinkle effects of UV (Kazue Tsukahara, Hidemi Nakagawa, MD, PhD, Shigeru Moriwaki, MSc, Yoshinori Takema, PhD, Tsutomu Fujimura, MSc, and Genji Imokawa, PhD The International Society of Dermatology It has been reported that it inhibits ultraviolet-B-induced wrinkle formation by an elastase-inhibiting herbal extract, implicating for the mechanism underlying elastase-associated wrinkles, and wound healing effect (CN199200108811).

On the other hand, phytoplankton of medicinal plants is a pharmaceutical technology that changes the original properties of medicines by processing the medicines based on oriental medicine theory. To reduce toxicity, to preserve the medicine's properties, to improve the medicine, and to improve the convenience of taking medicines, boil, steam, roast, roast, or boil it. Examples of the method of foojyeo include frying method for roasting a medicinal material, a method of frying a medicinal material with a certain amount of a liquid auxiliary material so that an auxiliary material is impregnated into the medicinal tissue, (Steam) method in which the liquid auxiliary material is added and mixed, heated in a suitable container, and steamed or steamed until a predetermined degree is reached. Fire intensity, and the type of equipment used. Some examples are as follows. It is a method of frying medicinal materials until it reaches a level defined by culture (fire) or artificial fire (fire). The liquorice method is a method of roasting a medicinal substance using about 15% of the alcohol and the method of boiling the medicinal substance using about 15% of alcohol. Even in the same medicinal plant, the properties of the raw material and the sown material may not be the same or may differ from each other. In addition, a combination of raw materials and timely combination of a combination of prescription drugs to increase the drug efficacy or toxicity to offset the treatment effect is maximized.

Accordingly, the present inventors have found that the extracts of Chrysanthemum morifolium extract, Neutrophil health extract, Chrysanthemum morifolium extract, Chrysanthemum morifolium extract and Chrysanthemum morifolium extract extracted from the crude herb extract, crude oil, health, ginseng or golden herbal extracts or corn oil, Inhibiting inflammation-related factors and promoting cleavage and migration of dermal keratinocytes, thereby rapidly promoting the progression from the inflammation to the proliferation, and thus exhibiting wound healing efficacy, thus completing the present invention.

Accordingly, an object of the present invention is to provide an anti-inflammatory composition or composition for treating a wound containing a herbal medicine extract or a pomegranate extract of a medicinal herb.

In order to accomplish the above object, the present invention provides a method for preparing a herbal medicine comprising extracting at least one herbal medicine selected from the group consisting of ganoderma, corn oil, health, ginseng and gold; At least one forage extract selected from the group consisting of corn steep liquor extract, herbal health extract, corn steep liquor extract and prickly gold extract; Or a mixture of the herbal medicine extract and the feces extract, as an active ingredient.

The composition for external application for skin is characterized in that it is used for anti-inflammation or wound healing.

The extract of Phytophthora infestans of the present invention, which is an extract of Phytophthora infestans, Healthy, Ganoderma and Golden herbal extracts or Cornus japonica, Healthy, Ganoderma and Golden Foam extracts, Herbal Extracts, Herbal Extracts, Herbal Extracts and Herbal Extracts have antiinflammatory and wound healing efficacies see. Therefore, the extract can be effectively used as a component that provides excellent anti-inflammatory or wound healing effects by being contained in cosmetic compositions or pharmaceutical compositions.

The composition of the present invention comprises at least one herbal medicine extract selected from the group consisting of jade, corn oil, health, manure and gold; One or more forage extracts selected from the group consisting of corn oil, health, manure and gold processed using fogue; Or a mixture of the herbal medicine extract and the feces extract as an active ingredient. The fogue is made by the method of main examination, cleanliness, runner and the like. Specifically, the maturity oil and the manure are the method of main examination, the health is the method of cleanliness, and the gold is the method of the runner.

The extract contained in the present invention is obtained by using water or an organic solvent from marine oil, health, manure, and gold each of which has been processed by a foaming method such as gypsum, acidulant, health, manure and gold, The organic solvent may be at least one selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate and chloroform, or a mixed solvent of these organic solvents and water may be used. Preferably, 80% ethanol is used.

The composition of the present invention contains the above herbal medicine extract in an amount of 0.001 to 15% by weight based on the total weight of the composition, and the content means the total content of the extract contained in the composition. When the content is less than 0.001% by weight, the effect is not confirmed. When the content is more than 15% by weight, the effect of the extract is not increased.

The composition according to the present invention may be provided for the treatment of anti-inflammatory or wound and tissue damage to which the composition is applicable may include, for example, scratches, cuticles, torn wounds, torn wounds, compression wounds, stretch wounds, Burn injury, burn injury, sun burn, chemical burn, surgical wound, surgical intervention, medical intervention, host rejection after cell, tissue or organ transplantation, medication But are not limited to, effects, pharmacological side effects, pressure ulcers, radiation damage, skin wounds due to cosmetics, developmental processes, maturation processes (such as acne), genetic abnormalities, abnormal development,

The composition of the present invention may be prepared in the form of a pharmaceutical composition containing the effective amount of the medicinal herb extract described above and may contain one or more non-toxic, pharmaceutically acceptable carriers, adjuvants or diluents or other active ingredients.

In addition, the composition of the present invention can be formulated in the form of an external preparation for skin using a pharmaceutically acceptable carrier and an excipient by a known method.

The composition according to the present invention may be in the form of a solution, a suspension or an emulsion in an oil or an aqueous medium, or in the form of a dry powder to be dissolved in sterile, exothermic water removed before use, have.

The composition of the present invention is in the form of a water-in-oil type emulsion in an emulsion, and can be formulated into cosmetic liquids, creams and the like. A water-in-oil type emulsion is prepared by emulsifying a mineral oil such as vegetable oil or liquid paraffin as an oil phase and a natural acid phospholipid such as soy bean lecithin and an ester of anhydrous hyssitol or fatty acid such as sorbitan monooleate And hexylol anhydride such as dioxyethylene sorbitol monooleate, and condensation products of a partial ester derived from a fatty acid with ethylene oxide are emulsified to emulsify the active ingredient.

Hereinafter, the present invention will be described in more detail with reference to the following examples and test examples. However, these examples and test examples are provided only to aid understanding of the present invention, and the scope of the present invention is not limited to these examples.

[Example 1] Production of Chinese medicinal herb (raw)

Five liters of 80% ethanol aqueous solution was added to 1 kg of each of dry crumbs, cornstarch, health, manure, and gold, refluxed three times, and then immersed at 15? For 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain herbal medicine extracts for each medicinal herb.

[Example 2-1] Production of acid fermentation extract

1 kg of dried cornstarch oil was soaked in a certain amount of sulfur, soaked in a steamer, steamed for 30 minutes to 2 hours, and then dried in the shade. To this was added 5 liters of an aqueous 80% ethanol solution, refluxed three times, and then immersed at 15 DEG C for 1 day. Thereafter, the residue and filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain an extract of corn oil.

[Example 2-2] Preparation of Healthy Herb Extract

1kg of clean health was put into a container of supernatant and fried at 100 ~ 160 ℃ for 10 minutes ~ 1 hour. To this was added 5 liters of an aqueous 80% ethanol solution, refluxed three times, and then immersed at 15 DEG C for 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain a healthy puree extract.

[Example 2-3] Preparation of Ganoderma lucidum extract

1kg of dried manure was soaked in a certain amount of sulfur, soaked in a steamer, steamed for 30 minutes to 2 hours, and then dried in the shade. To this was added 5 liters of an aqueous 80% ethanol solution, refluxed three times, and then immersed at 15 DEG C for 1 day. Thereafter, the residue and filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain a gingival extract.

[Example 2-4] Preparation of golden raspberry extract

1kg of clean gold was sprinkled evenly with a certain amount of sulfur (10 ~ 30%) and soaked so that it was damp enough to absorb all of the wine. The resultant was put in a suppository container and roasted at 100-160 ° C for 10 minutes to 1 hour and then dried in the shade. To this was added 5 liters of an aqueous 80% ethanol solution, refluxed three times, and then immersed at 15 DEG C for 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain a golden runner extract.

[Test Example 1] Wound treatment activity of herbal extracts through gross morphology

Human keratinocyte HaCaT was purchased from Korean Cell Line Bank (Seoul, Korea). HaCaT was cultured in an animal cell incubator at 37 ° C, 5% CO ₂ feeding conditions in a laboratory using DMEM medium containing 10% (v / v) FBS, penicillin 100 U / ml and streptomycin 100 μg / ml of antibiotics Respectively. HaCaT cells prepared at a concentration of 1.5 x 10 ⁶ per well were incubated for 24 hours to form a monolayer of cells and then induced " scratch-damage " with a p200 pipette tip. "Scratch-damaged" was again cultured for 24 hours or 48 hours in a culture medium containing the herbal medicines of Example 1 or the herbal extracts of Examples 2-1 to 2-4.

In the present invention, the in vitro wound healing assay was used to measure the amount of mossy, corn oil, health, manure, and herbal medicine extracts at a concentration of 10 ppm in a " scratch- After 24 hours and 48 hours of treatment, scratches were recovered. The control group was treated with the same amount of DMSO in which herbal medicinal herb extract was dissolved. The results are shown in Fig.

1, in the case of the control group, the scratch area with the elapse of time was not substantially reduced, but the "scratch-damage" of the cells treated with the medicinal herb extract was recovered after 24 hours and 48 hours to confirm that the scratch area was reduced .

In order to confirm the wound healing effect of herbal medicine extracts fabricated by embryo processing, extracts of herbal medicine extracts and herbal medicine extracts of each medicinal herb were treated and compared. The extracts of corn oil, health, manure and golden herb extracts and extracts of corn oil, herbicide, acacia, and quercetin were treated for 48 hours at a concentration of 10 ppm. The control group was treated with the same amount of DMSO in which the herbal medicinal herb extract was dissolved. The results are shown in FIG.

FIG. 2 shows that the scraped area of the cells treated with the extract of the herbal medicine processed with the forage treatment is much reduced, and the case of treating the herbal extract of the herbal medicine is more effective than the treatment of the herbal medicine extract of the herbal medicine The speed is much better.

In order to confirm the change by the combination prescription of medicinal herbs, a mixture prepared by mixing the raw medicinal herb extract and the phytase extract of each herbal medicines in a ratio of 1: 1: 1: 1: 1 was prepared. The combined prescription of 1ppm and 10ppm of each herbal medicinal plant extract was treated at a concentration of 10ppm for 24 hours, and the degree of recovery of the scratch scar was confirmed. The results are shown in FIG.

FIG. 3 shows that the use of the herbal medicine extract of the combination prescription has a better recovery rate of "scratching-damage" than the case of using one kind of extract, and has a synergistic effect according to the combination prescription. Effect can be obtained.

[Test Example 2] Determination of anti-inflammatory activity and keratinocyte-receptor-inducing effect of herbal medicine extracts and pomea extracts of herbal medicines through RT-PCR analysis

Human keratinocyte HaCaT was purchased from Korean Cell Line Bank (Seoul, Korea). HaCaT was cultured in an animal cell incubator at 37 ° C, 5% CO ₂ feeding conditions in a laboratory using DMEM medium containing 10% (v / v) FBS, penicillin 100 U / ml and streptomycin 100 μg / ml of antibiotics Respectively. HaCaT cells prepared at a concentration of 1.5 x 10 ⁶ per well were adapted for 3 hours in media not containing FBS. Then, the herbal extracts were pretreated at a concentration of 10 ppm for 2 hours, added with LPS at a concentration of 1 / / ml and further cultured for 8 hours. Total RNA was extracted with TRIzol ™ (GIBCO BRL, MD, USA) and stored at -80 ° C.

1 쨉 g of total RNA was added to ₂₅ 쨉 l of a reverse transcription reaction buffer containing 50 mM Tris-HCl (Tris-HCl, pH 8.3), 75 mM KCl, 3 mM MgCl ₂ , 0.1 M DTT, 10 mM dNTP, 40 units / R RNase inhibitor, (DT) ₁₆ primer and a 200 unit SuperScript II (SuperScript II, GiboBRL) reverse transcription polymer were added and reacted at 42 ° C for 1 hour. 2.5 μl of the reverse reaction solution was added to the AmpliTaq DNA polymerase (0.04 U, Perkin Elmer, Shelton, CT), 50 mM Tris (pH 8.3), 0.25 mg / ml bovine serum albumin , 3 mM MgCl ₂ , 0.25 mM dNTPs and 1 / 50,000 dilution of SYBR Green I (Molecular Probes, Eugene, Ole Molecular Probes, Eugene, OR)), and 10 μM of primer 30 cycles of 94 ° C for 30 seconds, 53 ° C for 30 seconds, and 72 ° C for 1 minute were performed.

Relative mRNA levels were analyzed by measuring SYBR Green I fluorescence changes using ICycler software. Each primer was prepared based on published SCI-grade papers by analyzing a substance having wound healing efficacy and inflammation-inhibiting function. The nucleotide sequences of the primers are shown in Table 1 below. Quantitative expression levels of genes were corrected using GAPDH (glyceraldehyde 3-phosphate dehydrogenase) as an internal standard.

The primer sequences used in PCR gene Primer sequence Size (bp) Denaturation temperature
(° C) Annealing temperature
(° C) Extended temperature
(° C) GAPDH 5'-ATC CCA TCA CCA TCT TCC AG-3 ' 579 94 58 72 5'-CCT GCT TCA CCA CCT TCT TG-3 ' iNOS 5'-ATG TCC GAA GCA AAC ATC AC-3 ' 401 94 58 72 5'-TAA TGT CCA GGA AGT AGG TG-3 ' IL1-beta 5'- TGC AGA GTT CCC CAA CTG GTA CAT C-3 ' 387 94 58 72 5'-GTG CTG CCT AAT GTC CCC TTG AAT C-3 ' TNF-a 5'-CCT GTA GCC CAC GTC GTA GC-3 ' 374 94 58 72 5'-TTG ACC TCA GCG CTG AGT TG-3 ' FGFR2-IIIb 5'-ACT CGG GGA TAA ATA GTT CCAA-3 ' 357 94 60 70 5'-CCT TAC ATA TAT ATT CCC CAG CAT-3 '

In vitro cell line model of LPS-induced in vitro cell line model, inflammatory factors that play an important role in inflammation during wound healing process have been reported in many experimental papers, such as iNOS, IL- Lt; / RTI > and < RTI ID = 0.0 > TNF-a < / RTI >

iNOS, IL-1 [beta] and TNF-alpha are variously expressed genes in lipopolysaccharide (LPS) -induced inflammatory responses. In the present invention, iNOS, a factor involved in proinflammatory mediator NO, RT-PCR analysis was performed to examine the effect of herbal extracts on the expression of IL-1β and TNF-α genes, and the results of each PCR product were quantified with respect to GAPDH. The results are shown in FIG. Significant levels of Tukey HSD post hoc test followed by one-way ANOVA were expressed as LPS + versus ** P <0.01, * P <0.05.

As shown in FIG. 4, LPS-treated cells showed overexpression of each gene and treatment of herbal medicine extract strongly inhibited iNOS, IL-1β, and TNF-α gene expression, compared to cells not treated with LPS have. Also, it can be confirmed that the extract exhibits more superior efficacy than the conventional herbal medicine extract.

On the other hand, the expression of the keratinocyte growth factor receptor (FGFR2-IIIb) gene has been reported to be correlated with cell proliferation effect ( Nagy N, Bata-Csorgo Z, Kopasz N, Szeg C, Pivarcsi A, Korecka Dobozya , Kemeny L, Szell M. Expression of keratinocyte growth factor receptor (FGFR2-IIIb) correlates with the high proliferative rate of HaCaT keratinocytes Exp Dermatol 2006 Aug; 15 (8): 596-605). The expression level of the keratinocyte growth factor receptor (FGFR2-IIIb) gene was measured in the same manner as described above, and the effect of the herb extract on the expression of the keratinocyte growth factor receptor (FGFR2-IIIb) gene was confirmed. Is shown in Fig.

FIG. 5 shows that the expression of the keratinocyte growth factor receptor (FGFR2-IIIb) gene is much higher when the herbal medicine extract is treated as compared to the control group not treated with the herbal medicine extract.

As described above, the herbal medicine extract used as an active ingredient in the present invention inhibits inflammation-related factors and promotes re-epithelization, thereby facilitating the progression from the inflammation to the proliferation during the wound healing process, stimulating cell proliferation during the proliferation period, Helps the maturation of connective tissue cells by helping to release therapeutic factors.

Therefore, the herbal medicine extract according to the present invention has an effect of reducing the treatment time by giving a little help to the initial stage of inflammation and mature stage as a whole during the overall wound healing process. In the present invention, the composition containing the herbal medicine extract as an active ingredient is anti- Or as a wound healing agent.

[Formulation Example 1] Production of nutritional lotion containing herbal extract

The nutritional lotion according to the present invention was prepared with the composition shown in Table 1 below. Wherein the herbal medicine extract comprises at least one herbal medicine extract selected from the group consisting of ginseng, corn oil, health, ginseng and gold, or at least one herbal medicine extract selected from the group consisting of corn steep liquor extract, , Or a mixture of the herbal medicine extract and the feces extract.

ingredient Content (% by weight) Purified water To 100 glycerin 8.0 Butylene glycol 4.0 Hyaluronic acid extract 5.0 Beta Glucan 7.0 Carbomer 0.1 Herbal medicine extract 0.05 Caprylic capric triglyceride 8.0 Squalane 5.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Cetearyl alcohol 1.0 antiseptic Suitable amount incense Suitable amount Pigment Suitable amount Triethanolamine 0.1

[Formulation Example 2] Preparation of nutritional cream containing herbal extract

The nutritional creams according to the present invention were prepared with the compositions shown in Table 2 below. Wherein the herbal medicine extract comprises at least one herbal medicine extract selected from the group consisting of ginseng, corn oil, health, ginseng and gold, or at least one herbal extract selected from the group consisting of corn steep liquor extract, , Or a mixture of the herbal medicine extract and the feces extract.

ingredient Content (% by weight) Purified water To 100 glycerin 3.0 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 Herbal medicine extract 3.0 Caprylic capric triglyceride 3.0 Squalane 5.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 antiseptic Suitable amount incense Suitable amount Pigment Suitable amount Triethanolamine 0.1

[Formulation Example 3] Preparation of massage cream containing herbal extract

The massage cream according to the present invention was prepared with the composition shown in Table 4 below. Wherein the herbal medicine extract comprises at least one herbal medicine extract selected from the group consisting of ginseng, corn oil, health, ginseng and gold, or at least one herbal medicine extract selected from the group consisting of corn steep liquor extract, , Or a mixture of the herbal medicine extract and the feces extract.

ingredient Content (% by weight) Purified water To 100 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 45.0 Beta Glucan 7.0 Carbomer 0.1 Herbal medicine extract 1.0 Caprylic capric triglyceride 3.0 Wax 4.0 Cetearyl glucoside 1.5 Sesquioleic acid sorbitan 0.9 Vaseline 3.0 antiseptic Suitable amount incense Suitable amount Pigment Suitable amount paraffin 1.5

[Formulation Example 4] Preparation of pack containing herbal extract

Packs according to the present invention were prepared with the compositions of Table 5 below. Wherein the herbal medicine extract comprises at least one herbal medicine extract selected from the group consisting of ginseng, corn oil, health, ginseng and gold, or at least one herbal medicine extract selected from the group consisting of corn steep liquor extract, , Or a mixture of the herbal medicine extract and the feces extract.

ingredient Content (% by weight) Purified water To 100 glycerin 4.0 Polyvinyl alcohol 15.0 Hyaluronic acid extract 5.0 Beta Glucan 7.0 Allantoin 0.1 Herbal medicine extract 0.5 Nonyl phenyl ether 0.4 Polysorbate 60 1.2 antiseptic Suitable amount incense Suitable amount Pigment Suitable amount ethanol 6.0

[Formulation Example 5] Preparation of ointment containing herbal extract

Ointment in the external preparation for skin according to the present invention was prepared with the composition shown in Table 6 below. Wherein the herbal medicine extract comprises at least one herbal medicine extract selected from the group consisting of ginseng, corn oil, health, ginseng and gold, or at least one herbal medicine extract selected from the group consisting of corn steep liquor extract, , Or a mixture of the herbal medicine extract and the feces extract.

ingredient Content (% by weight) Purified water To 100 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 15.0 Beta Glucan 7.0 Carbomer 0.1 Herbal medicine extract 1.0 Caprylic capric triglyceride 3.0 Squalane 1.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Cetearyl alcohol 1.0 antiseptic Suitable amount incense Suitable amount Pigment Suitable amount Wax 4.0

FIG. 1 is a photograph showing the wound healing effect of the ginseng, corn oil, health, manure, and herbal medicine extracts using an in vitro wound healing assay.

FIG. 2 is a graph showing the effect of wound healing on increased corn oil, health, manure, and gold due to forage using an in vitro wound healing assay.

FIG. 3 is a graph showing an increase in wound healing efficacy by a combination of a transdermal drug delivery system, a crude medicinal herb, a health supplement, a manure, and a gold using an in vitro wound healing assay.

FIG. 4 is a graph showing the results of measuring the expression levels of GAPDH, iNOS, IL-1β and TNF-α mRNAs in HaCaT cells induced by LPS.

FIG. 5 is a graph showing the effect of inducing the expression of the keratinocyte growth factor receptor (FGFR2-IIIb) gene by lysozyme, acid fermentation, health, Ganoderma lucidum,

Claims (4)

At least one herbal medicine extract selected from the group consisting of jade, health, manure, and gold; At least one forage extract selected from the group consisting of herbal health extracts, corn borer extracts and prunus golden extracts; or A mixture of the herbal medicine extract and the feces extract; As an active ingredient. The composition for external application for skin according to claim 1, wherein the active ingredient is contained in an amount of 0.001 to 15% by weight based on the total weight of the composition. delete The composition for external application for skin according to claim 1, wherein the composition is for wound healing.

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