KR100585613B1 - External application composition containing ?-1,3-glucan and ginseng saponin - Google Patents

Abstract

The present invention relates to an external preparation composition containing beta-1,3-glucan and ginseng saponin. In more detail, by combining beta-1,3-glucan and ginseng saponin in an optimal ratio, it is possible to provide an external preparation composition having excellent skin wrinkle improvement and elasticity enhancing effects such as skin cell proliferation and collagen fiber synthesis promotion.

Beta-1,3-glucan, ginseng saponin, skin aging prevention, wrinkle improvement, elasticity improvement

Description

External application composition containing β-1,3-glucan and ginseng saponin}

The present invention relates to an external composition for skin containing beta-1,3-glucan and ginseng saponin.

The skin is the body's primary barrier, protecting the organs of the body from stimuli of the external environment, such as changes in temperature and humidity, ultraviolet rays, and pollutants. However, internally, as hormones age, the secretion of various hormones that regulate metabolism decreases, and the function and function of cells of immune cells decreases, thus reducing the biosynthesis of immune proteins and bioconstituent proteins necessary for the living body. As environmental pollution, such as increased ultraviolet rays of the sun's rays reaching the skin due to the destruction of the ozone layer, becomes more severe, the normal functioning of the skin is caused by excessive physical and chemical irritation and stress caused by the increase of free radicals and free radicals. Decreases and accelerates the aging of the skin to damage the skin. In order to prevent this phenomenon and maintain healthy and beautiful skin, by using physiologically active substances obtained from various animals, plants, and microorganisms in the past, cosmetics are used to maintain the unique function of the skin and to activate skin cells. Efforts have been made to effectively suppress

However, existing raw materials have various problems such as insufficient efficacy or cause skin side effects.

Therefore, while the present inventors are studying a substance capable of inhibiting phenomena such as a decrease in the regeneration of skin cells and a decrease in collagen synthesis, which are major indicators of skin aging, beta-1,3-glucan is a proliferation of collagen fibers and collagen fibers. It has been found that it has the action of promoting the function of the skin, such as promoting the production of the sun, healing of sunburn, etc., and has applied for a patent for an external preparation composition having an excellent effect on preventing skin aging and healing of skin damage containing the same (Korean patent) Application 98-49026).

However, in order to further enhance the above-mentioned efficacy of beta-1,3-glucan, the present inventors have studied and examined more extensively about the combination effect with existing skin anti-aging substances and the composition of a more effective anti-aging external preparation. As a result, ginseng is known to promote blood circulation in the capillaries of the skin to facilitate the supply of nutrients to the epidermis, promote the synthesis of glycosaminoglycans in the dermal connective tissue, and have an anti-inflammatory effect. When both saponin and branched-beta-1,3-glucan are used simultaneously, they find that the combination composition effectively suppresses skin aging, improves skin elasticity, and is a skin external preparation that is excellent in improving wrinkles and improving skin elasticity. Invented the composition.                         

Accordingly, it is an object of the present invention to provide an external preparation composition containing beta-1,3-glucan and ginseng saponin to prevent skin aging.

That is, an object of the present invention is to use the excellent skin wrinkle improvement and elasticity promoting function of beta-1,3-glucan and ginseng saponin, and to provide an external composition for skin by utilizing the complex synergistic effect of these components. .

In order to achieve the above object, the external preparation composition according to the present invention contains beta-1,3-glucan in an amount of 0.00001 to 10% by weight, and ginseng saponin of 0.00001 to 10% by weight based on the total weight of the composition. It is characterized by containing in an amount.

Beta-1,3-glucan used in the present invention is particularly suitable for high purity glucan with a purity of 98% or more obtained by liquid culture of Schizopyllum commune mycelium, which has an anti-aging and damaged skin healing effect. In addition to the skirt mushroom, beta-1,3-glucan obtained by culturing Ganoderma lucidum mushroom, Shiitake mushroom, shiitake mushroom, yeast, barley and the like can be used.

On the other hand, ginseng saponin is the main chemical component of ginseng, called ginsenosides, unlike the saponin components contained in other plants can exhibit a variety of biological activities with little toxicity, such as hemolytic action.

The external preparation composition of the present invention contains beta-1,3-glucan and ginseng saponin in an amount of 0.00001 to 10% by weight, preferably 0.001 to 5% by weight based on the total weight of the composition. The reason for limiting the content as described above is because of the correlation between economic efficiency and efficacy. When the content is more than 10%, the effect is no longer increased, so there is no economic advantage, and when the content is less than 0.00001%, sufficient effect This is because there is a problem that does not give.

The external preparation composition for skin of the present invention is not particularly limited in its formulation, and may be, for example, a cosmetic composition having a formulation of a flexible cosmetic water, a nourishing cosmetic water, a massage cream, a nourishing cream, a pack, a gel, or a skin adhesive type cosmetic. And may also be transdermal formulations such as lotions, ointments, gels, creams, patches or sprays.

In addition, in the external preparation composition of each formulation, other components in addition to the above-mentioned ginseng saponin and beta-1,3-glucan can be appropriately selected and blended by those skilled in the art without difficulty according to the formulation or purpose of use of other external preparations.

Examples and test examples of the present invention will be described in more detail the configuration and effect of the present invention. However, the examples or test examples of the present invention are intended to explain the present invention in more detail, and the present invention is not limited to the examples or test examples.

<Test Example 1>

Measurement of proliferative effect of human fibroblasts

Beta-1,3-glucan used in Test Examples and Examples of the present invention is a high purity beta-1,3-glucan obtained by liquid culture of Schizopyllum commune mycelium with a purity of 98% or higher, and ginseng saponin is Korean ginseng It was supplied by the Corporation (manufactured by Korea Ginseng and Tobacco Research Institute) and had a content of irradiated ponin 9.9%.

Ginseng saponin and the beta-1,3-glucan were used in the test by diluting to the concentration of Table 1 using a culture medium of fibroblasts.

A group treated with ginseng saponin (Sample 1) diluted to 0.01% in human fibroblasts collected directly from the human body, a group treated with beta-1,3-glucan (Sample 2) diluted to 0.04%, and a beta- The proliferative effect of fibroblasts in the group treated with the composition (Samples 3 to 7) in which 1,3-glucan and ginseng saponin were mixed in appropriate amounts was measured.

Sample concentration (%) Sample number Beta-1,3-glucan Ginseng saponin  1 (Ginseng Saponin) 0 0.01  2 (beta-1,3-glucan) 0.04 0  3 0.004 0.001  4 0.004 0.05  5 0.04 0.01  6 0.4 0.1  7 1.0 0.5

1) Test method

Human fibroblasts incubated in Dulbecco's Modified Eagle's Media (DMEM) medium containing 2.5% fetal calf serum were dispensed at 96 cells / well in a 96-well microtiter plate. After incubating the samples of Table 1 by treating 100 μl twice a day for 3 days, 0.2% MTT (3- [4,5-dimethylthiazol-2-yl] -2, 5-phenyltertazolium bromide) solution was prepared. 50 μl per well was added, followed by further incubation at 37 ° C. for 4 hours, and then DMSO (dimethyl sulfoxide) was produced from the resultant formazan (product produced by the reaction of dehydrogenase of mitochondria with MTT). ) Was dissolved. The absorbance of the dissolved formazan was measured at 570 nm using a microplate reader. At this time, the group not treated with the sample was used as a control.

The absorbance measured in this way can be used to determine the fibroblast proliferation promoting effect of the samples, since the absorbance also increases when the cells are grown and the amount of cells increases, so the absorbance is proportional to the amount of cells. The proliferation effect was calculated by comparing the absorbance of the control group with the absorbance of each sample by the following method.

2) Experiment result

The proliferative effect in each sample is shown in Table 2. As can be seen in Table 2, fibroblast proliferation was found when treated with each sample. When beta-1,3-glucan and ginseng saponin were used simultaneously, the proliferation of fibroblasts was higher than when each was used alone. It can be seen that the effect is increased. In particular, when sample 7, i.e., beta-1,3-glucan and ginseng saponin, was used at concentrations of 1.0% and 0.5%, respectively, the proliferative effect of fibroblasts reached 78%.

sample Proliferation effect (%)  1 (Ginseng Saponin) 20  2 (beta-1,3-glucan) 30  3 25  4 38  5 68  6 70  7 78  Control 0

<Test Example 2>

Measurement of proliferative effect of human epidermal cells

Epidermal cells of humans directly collected from the human body were treated with the same method in Test Example 1, and the proliferative effect of the epidermal cells was measured by the same method.

The results are shown in Table 3. As shown in Table 3, proliferation of epidermal cells was found when treated with each sample as in Test Example 1, but when beta-1,3-glucan and ginseng saponin were used simultaneously, It can be seen that the proliferation of epidermal cells is elevated. In particular, when sample 7, i.e., beta-1,3-glucan and ginseng saponin, was used at concentrations of 1.0% and 0.5%, respectively, the proliferation effect of epidermal cells reached 55%.

Epidermal cell proliferation effect sample Proliferation effect (%)  1 (Ginseng Saponin) 15  2 (beta-1,3-glucan) 25  3 20  4 28  5 53  6 50  7 55  Control 0

<Test Example 3>

Measurement of Collagen Production Promoting Effect in Human Fibroblasts

Human fibroblasts collected directly from the human body were treated with the same samples as in Test Example 1 to determine the effect of collagen protein production.

1) Test Method

After culturing human fibroblasts in a 24-well plate incubator, each sample of Table 1 above was added to the culture medium. On the third day of culture, 0.5 ml of DMEM medium containing 10% fetal calf serum was added to each well, and then 10 μCi of L [2, 3, 4, 5- ³ H] -proline was added. After 24 hours, the media and cells contained in each well were scraped, washed in 5% trichloroacetic acid (TCA) solution, and dispensed into two test tubes. 1 unit / μl of type I collagenase was added to each test tube, one was incubated at 37 ° C. for 90 minutes, and the other test tube was stored at 4 ° C. Thereafter, 0.05 ml of 50% TCA was added to all test tubes, and the mixture was allowed to stand at 4 ° C. for 20 minutes, followed by centrifugation at 12,000 rpm for 10 minutes, respectively. (dpm; decay per minute) to obtain a collagen biosynthesis value (RCB; Relative Collagen Biosynthesis) based on the following equation (2).

The above formula is shown in "Webster & Harvey, 1979, Anal. Biochem 96, 220-224" as a general formula for obtaining collagen biosynthesis values. The value calculated in the above formula represents the amount of collagen production increased in units of% compared to the control for each sample.

2) Test result

The experimental results are shown in Table 4. As can be seen in Table 4, beta-1,3-glucan and ginseng saponin at the same time, it can be seen that the collagen production effect is increased than when using each alone.

Collagen Production Proliferation Effect sample Proliferation effect (%)  1 (Ginseng Saponin) 18  2 (beta-1,3-glucan) 27  3 15  4 29  5 55  6 59  7 55  Control 0

<Test Example 4>

Collagen production promoting effect through animal experiment

1) Preparation of oil in water (O / W) formulations of Examples 1 and Comparative Examples 1 to 3

The composition ratio of each component for manufacture of oil-in-water (O / W) formulation in Example 1 and Comparative Examples 1-3 is shown in Table 5.

The raw materials 1-5 were mixed, melt | dissolved at 70 degreeC, and it was set as the water part. On the other hand, raw materials 6-13 were melt | dissolved at 70 degreeC, and it was set as the oil part. The oil part was added to the water part, and then stirred with a homomixer to emulsify. After removing the bubbles, oil-in-water (O / W) formulation prepared by cooling to room temperature was used in Examples 1 and Comparative Examples 1-3.

2) Collagen Production Test Method

Hairless mice were irradiated with UV rays once daily for 8 weeks (UVB 70mJ / cm ² ), and then the groups to which the Comparative Examples 1 to 3 were applied were divided into 2 groups of 20 animals in each group. Prepared Example 1 and Comparative Examples 1 to 3 each day for 1 month to 100μl daily applied to the back area and biopsied by the H & E staining method (Kligman, 1982, JID 78, p181 ~ 189) to form histologically collagen The degree was measured.

In this test example, test animals irradiated UV light on hairless mice. Ultraviolet rays not only degrade collagen production but also promote collagen degradation, which is photoaging, which measures the degree of collagen production in tissues of hairless mice irradiated with UV light to detect collagen production under such UV-irradiated conditions.

3) results

Collagen production rate was calculated using an image analyzer. The collagen production rate was determined by quantifying the staining degree by analyzing the color of the H & E staining site.

The results are shown in Table 6. As shown in Table 6, it can be seen that the production of collagen is increased by the external preparation composition according to the present invention, which has an effect on skin protection.

 Collagen Production Rate Applicator Comparative Example 1 Comparative Example 2 Comparative Example 3 Example 1 Collagen production rate (%) 0 35 25 70

<Test Example 5>

Skin wrinkle improvement effect on human skin

1) Preparation of oil-in-water (O / W) formulations of Examples 2 and Comparative Examples 4-6

In Example 2 and Comparative Examples 4 to 6, the composition ratio of each component for preparing the O / W formulation is shown in Table 7 below.

Composition ratios of Comparative Examples 4 to 6 and Example 2 ingredient Example 2 Comparative Example 4 Comparative Example 5 Comparative Example 6  1.Purified water to 100 to 100 to 100 to 100  2.glycerine 8.0 8.0 8.0 8.0  3. Butylene glycol 4.0 4.0 4.0 4.0  4.hyaluronic acid extract 5.0 5.0 5.0 5.0  5.Carbomer 0.1 0.1 0.1 0.1  6.beta-1,3-glucan 1.0 0 1.0 0  7.Ginseng Saponin 0.5 0 0 0.5  8.Caprylic Capric Trigly Seride 8.0 8.0 8.0 8.0  9.Squalane 5.0 5.0 5.0 5.0  10.Cetearyl Glucoside 1.5 1.5 1.5 1.5  11.Sorbitan stearate 0.4 0.4 0.4 0.4  12.Cetearyl Alcohol 1.0 1.0 1.0 1.0  13.preservative Quantity Quantity Quantity Quantity  14. Incense Quantity Quantity Quantity Quantity  15.Triethanolamine 0.1 0.1 0.1 0.1

The raw materials 1-6 were mixed, melt | dissolved at 70 degreeC, and it was set as the water part. On the other hand, raw materials 7-14 were melt | dissolved at 70 degreeC, and it was set as the oil part. The oil part was added to the water part, stirred with a homomixer to emulsify, and the raw material 15 was added to increase. After removing the bubbles, the mixture was cooled to room temperature and used in Examples 2 and 4-6.

2) Wrinkle improvement effect test method

The skin wrinkle improvement effect of the cosmetics prepared in Comparative Examples and Examples above was compared. Wrinkle improvement effect of skin was measured by dividing nine groups of 180 women in their 30s to 40s. The test method is as follows.

After giving the products prepared in Examples 2 and Comparative Examples 4 to 6 in each tank and applying them once a day for 1 month, after 1 month, a replica was made using silicone and visiometer (SV500, Courage + Khazaka electronic GmbH, Germany) By measuring the depth of wrinkles by measuring the depth of wrinkles through image analysis using), the elasticity of the face area was measured by using a cutometer (SEM474, Courage + Khazaka electronic GmbH, Germany) that can measure skin elasticity. The degree of elasticity improvement was evaluated as compared with before application, and the moisturizing power was determined by using a coneometer (CM820 Courage + Khazaka electronic GmbH, Germany), and the questionnaire was used to determine whether the makeup was smooth and the makeup was well received.

3) results

The test results are shown in Table 8. The degree of improvement of the skin after application was expressed in% as compared to the state before application of each product, and the average was obtained.

 Clinical results after 1 month of use Comparative Example 4 Comparative Example 5 Comparative Example 6 Example 2  Wrinkle reduction (%) 5 40 25 88  Skin elasticity improvement degree (%) 5 35 20 80  Moisturizing capacity increase rate (%) 20 45 35 70  Smoothness increase (%) 18 40 25 75  Receiving degree of makeup well (%) 30 50 40 80

As can be seen from Table 8, it is apparent that the external preparation composition of the O / W formulation containing beta-1,3-glucan and ginseng saponin at the same time is effective in improving skin wrinkles and enhancing elasticity.

Hereinafter, on the basis of the results of the above test examples, by simultaneously containing beta-1,3-glucan and ginseng saponin, to present a cosmetic composition of various formulations that can provide a skin anti-aging effect. However, the composition of the present invention is not limited only to the following examples.

<Formulation Example 1> Softening Cosmetic (Skin Lotion)

Ingredient weight%  Ginseng saponin 0.5  Beta-1,3-glucan 1.0  Butylene glycol 2.0  Propylene glycol 2.0  Carboxy Vinyl Polymer 0.1  Fiji-12 nonylphenyl ether 0.2  Polysorbate 80 0.4  ethanol 10.0  Triethanolamine 0.1  Preservative, coloring, flavoring Quantity  Purified water to 100

<Formulation Example 2> Nourishing Cosmetic Water (Milk Lotion)

Ingredient weight%  Ginseng saponin 0.5  Beta-1,3-glucan 1.0  Beeswax 4.0  Polysorbate 60 1.5  Sorbitan sesquioleate 1.5  Liquid paraffin 0.5  Caprylic / Capric Triglycerides 5.0  glycerin 3.0  Butylene glycol 3.0  Propylene glycol 3.0  Carboxy Vinyl Polymer 0.1  Triethanolamine 0.2  Preservative, coloring, flavoring Quantity  Purified water to 100

Formulation Example 3 Nutrition Cream

Ingredient weight%  Ginseng saponin 1.0  Beta-1,3-glucan 5.0  Beeswax 10.0  Polysorbate 60 1.5  Sebum 60 Cured Castor Oil 2.0  Sorbitan sesquioleate 0.5  Liquid paraffin 10.0  Squalane 5.0  Caprylic / Capric Triglycerides 5.0  glycerin 5.0  Butylene glycol 3.0  Propylene glycol 3.0  Triethanolamine 0.2  Preservative, coloring, flavoring Quantity  Purified water to 100

Formulation Example 4 Massage Cream

Ingredient weight%  Ginseng saponin 1.0  Beta-1,3-glucan 3.0  Beeswax 10.0  Polysorbate 60 1.5  Sebum 60 Cured Castor Oil 2.0  Sorbitan sesquioleate 0.8  Liquid paraffin 40.0  Squalane 5.0  Caprylic / Capric Triglycerides 4.0  glycerin 5.0  Butylene glycol 3.0  Propylene glycol 3.0  Triethanolamine 0.2  Preservative, coloring, flavoring Quantity  Purified water to 100

<Formulation Example 5> Pack

Ingredient weight%  Ginseng saponin 0.5  Beta-1,3-glucan 1.0  Polyvinyl alcohol 13.0  Sodium Carboxymethyl Cellulose 0.2  glycerin 5.0  Allantoin 0.1  ethanol 6.0  Pingji-12 Nonylphenyl Ether 0.3  Polysorbate 60 0.3  Preservative, coloring, flavoring Quantity  Purified water to 100

<Formulation Example 6> Gel

Ingredient weight%  Ginseng saponin 0.05  Beta-1,3-glucan 0.1  Ethylenediamine Sodium Acetate 0.05  glycerin 5.0  Carboxy Vinyl Polymer 0.3  ethanol 5.0  Fiji-60 Cured Castor Oil 0.5  Triethanolamine 0.3  Preservative, coloring, flavoring Quantity  Purified water to 100

<Formulation Example 7> Ointment

Ingredient weight%  Ginseng saponin 2.0  Beta-1,3-glucan 10.0  Beeswax 10.0  Polysorbate 60 5.0  Sebum 60 Cured Castor Oil 2.0  Sorbitan sesquioleate 0.5  vaseline 5.0  Liquid paraffin 10.0  Squalane 5.0  Shea Butter 3.0  Caprylic / Capric Triglycerides 5.0  glycerin 10.0  Propylene glycol 10.2  Triethanolamine 0.2  Preservative, coloring, flavoring Quantity  Purified water to 100

<Formulation Example 8> Topical administration agent (gel ointment)

Ingredient weight%  Ginseng saponin 3.0  Beta-1,3-glucan 10.0  Polyacrylic Acid (Carbopol 940) 1.5  Isopropanol 5.0  Hexylene glycol 25.0  Triethanolamine 1.7  Deionized water to 100

<Formulation example 9> The agent for topical administration (patch)

Ingredient weight%  Ginseng saponin 1.0  Beta-1,3-glucan 3.0  Hexylene glycol 20.0  Diethylamine 0.7  Polyacrylic Acid (Carbopol 934P) 1.0  Sodium sulfite 0.1  Polyoxyethylene lauryl ether (E.O = 9) 1.0  Polyhydroxyethylenecetylstearyl ether (Cetomacrogol 1000) 1.0  Viscous Paraffin Oil 2.5  Caprylic Acid Ester / Capric Acid Ester (Cetiol LC) 2.5  Polyethylene Glycol 400 3.0  Deionized water to 100

As described above, the external preparation for skin containing beta-1,3-glucan and ginseng saponin provided by the present invention has an effect of improving skin wrinkles and improving skin elasticity and preventing skin aging.

Claims (5)

A skin external composition comprising beta-1,3-glucan in an amount of 0.01 to 10% by weight and ginseng saponin in an amount of 0.01 to 10% by weight, based on the total weight of the composition. According to claim 1, wherein the composition is an external composition, characterized in that the cosmetic composition having a formulation of softening, longevity, nutrition cream, massage cream, pack or gel. The composition of claim 1, wherein said composition has a transdermal formulation of a lotion, ointment, gel, cream, patch or spray. According to claim 1, wherein the beta-1,3-glucan is a topical composition, characterized in that it is derived from chima mushroom, Ganoderma lucidum mushroom, mushroom, shiitake, fungi, yeast or barley. The external preparation composition according to claim 1, wherein the external preparation composition is for skin wrinkle improvement, skin elasticity improvement or skin moisturizing.