KR100361433B1 - Anti-aging cosmetic compositions containing an Extract obtained from Panax ginseng - Google Patents

Anti-aging cosmetic compositions containing an Extract obtained from Panax ginseng Download PDF

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KR100361433B1
KR100361433B1 KR1019980019526A KR19980019526A KR100361433B1 KR 100361433 B1 KR100361433 B1 KR 100361433B1 KR 1019980019526 A KR1019980019526 A KR 1019980019526A KR 19980019526 A KR19980019526 A KR 19980019526A KR 100361433 B1 KR100361433 B1 KR 100361433B1
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extract
skin
ginseng
panax ginseng
meyer
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KR19990086516A (en
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오지연
박이근
송동혁
염명훈
조윤기
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주식회사 태평양
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783Angiosperms [Magnoliophyta]
    • A61K8/9789Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/08Anti-ageing preparations

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  • Dermatology (AREA)
  • Gerontology & Geriatric Medicine (AREA)
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Abstract

PURPOSE: A cosmetic composition containing an extract of Panax ginseng C.A Meyer for prevention of skin aging is provided. The Panax ginseng C.A Meyer extract has effects on proliferation of skin cell and facilitation of collagen biosynthesis, so that the skin aging can be effectively prevented. CONSTITUTION: A cosmetic composition for prevention of skin aging contains an extract of Panax ginseng C.A Meyer, wherein the amount of the Panax ginseng C.A Meyer extract contained is 10 superscript -8(0.00000001) to 10 wt.%; the Panax ginseng C.A Meyer extract is prepared by the steps of: extracting Panax ginseng C.A Meyer with ethanol; heat-extracting insoluble remnants of Panax ginseng C.A Meyer and filtering the extract; and adding ethanol into the filtered extract to obtain the precipitated ginseng polysaccharides.

Description

인삼추출물을 함유하는 피부 노화방지용 화장료 조성물 {Anti-aging cosmetic compositions containing an Extract obtained from Panax ginseng}Anti-aging cosmetic compositions containing an Extract obtained from Panax ginseng}

본 발명은 인삼의 추출물을 함유하는 피부 노화방지용 화장료 조성물에 관한 것으로, 보다 상세하게는 피부 세포의 증식 및 콜라겐 생합성 촉진 효과를 갖는 인삼(Panax ginsengC.A Meyer)의 추출물을 함유함으로써 피부 노화방지 효과를 갖는 화장료 조성물에 관한 것이다.The present invention relates to a skin anti-aging cosmetic composition containing an extract of ginseng, and more particularly to the skin anti-aging effect by containing an extract of ginseng ( Panax ginseng CA Meyer) having the effect of promoting the proliferation of skin cells and collagen biosynthesis It relates to a cosmetic composition having.

피부는 인체의 일차 방어막으로서 체내의 제기관을 온도 및 습도 변화와 자외선, 공해물질 등 외부환경의 자극으로부터 보호해 주며, 체온조절 등의 생체 항상성 유지에 중요한 역할을 하고 있다. 그러나, 외부로부터 받는 과도한 물리적, 화학적 자극 및 스트레스, 영양결핍 등은 피부의 정상기능을 저하시키고 탄력손실, 각질화, 주름생성 등의 피부 노화현상을 촉진시키게 되는데, 이러한 현상을 방지하고 보다 건강하고 탄력있는 피부를 유지하기 위하여 종래 각종 동물, 식물, 미생물 등으로부터 얻은 생리활성물질들이 강화된 화장품을 사용함으로써 피부의 고유기능을 유지시키고 피부세포를 활성화시켜 피부노화를 효과적으로 억제하기 위한 노력이 있어 왔었다. 그러나, 이러한 기존의 화장품 원료들은 대부분 그 효능이 미진하거나 피부 부작용을 유발하는 등 여러 가지 문제점을 가지고 있다.Skin is the body's primary protective film that protects internal organs from changes in temperature and humidity, and from stimuli from the external environment such as ultraviolet rays and pollutants, and plays an important role in maintaining homeostasis such as body temperature control. However, excessive physical and chemical stimuli, stress, and malnutrition from the outside can reduce the normal function of the skin and promote skin aging such as loss of elasticity, keratinization and wrinkle formation. In order to maintain the skin, there have been efforts to effectively inhibit skin aging by maintaining skin's intrinsic function and activating skin cells by using cosmetics enhanced with bioactive substances obtained from various animals, plants, and microorganisms. However, these conventional cosmetic raw materials have a number of problems, such as most of them are ineffective or cause skin side effects.

이에, 본 출원인들은 피부에 안전하면서도 피부노화의 주요 원인인 피부의 각질형성세포, 섬유아세포의 재생감소 그리고 이로부터 생성되는 콜라겐의 생합성량 감소를 효과적으로 억제할 수 있는 피부 노화방지용 화장료를 제공하기 위하여 예의 연구하던 중, 인삼(Panax ginsengC.A. Meyer)의 추출물, 특히 인삼의 에탄올 불용부분으로부터 추출한 인삼 다당체가 피부노화를 방지할 수 있는 효과가 있음을 발견하고 본 발명을 완성하게 되었다.Accordingly, the present applicants provide a skin anti-aging cosmetic that can effectively inhibit skin keratinocytes, fibroblasts regeneration and collagen produced from reducing the amount of biosynthesis, which is a major cause of skin aging. During the intensive studies, the present invention was completed by finding that the extract of Panax ginseng C.A.Meyer, especially the ginseng polysaccharide extracted from the ethanol insoluble portion of ginseng, can prevent skin aging.

따라서, 본 발명의 목적은 피부 노화방지용 화장료 조성물을 제공하는 것이다.Accordingly, it is an object of the present invention to provide a cosmetic composition for preventing skin aging.

상기한 목적을 달성하기 위하여, 본 발명의 피부 노화방지용 화장료 조성물은 인삼 추출물을 조성물 총 중량에 대하여 건조 중량으로 10-8∼101중량%의 양으로 함유함을 특징으로 한다.In order to achieve the above object, the skin anti-aging cosmetic composition of the present invention is characterized by containing ginseng extract in an amount of 10 -8 to 10 1 % by weight in dry weight based on the total weight of the composition.

상기한 본 발명의 목적, 그외의 목적, 특징 및 장점은 하기 발명의 상세한 설명으로부터 당업자에게 명백하게 드러날 것이다.The above and other objects, features and advantages of the present invention will become apparent to those skilled in the art from the following detailed description.

이하, 본 발명을 보다 상세하게 설명한다.Hereinafter, the present invention will be described in more detail.

인삼(Panax ginsengC.A. meyer)은 오가피과 인삼속에 속하는 식물로 한국, 중국, 일본 등지에서 2,000여년 전부터 사용되어 온 생약으로, 경험적으로 질병을예방하고 수명을 연장시킬 목적으로 사용되어 왔으며, 피부의 기능을 항진시켜 피부를 건강하게 유지시키는 효과가 있다고 알려져 있다. 또한, 인삼의 추출물은 항보체 활성, 항암작용, 혈당강하작용, 항궤양 작용 그리고 면역증강 작용이 있는 것으로 최근에 많은 연구를 통해 보고되고 있다. 이러한 연구는 주로 인삼을 에탄올 수용액으로 추출한 후, 이를 용매분획을 실시하여 실리카겔 칼럼크로마토그래피법 또는 C-18 칼럼크로마토그래피법을 이용하여 단일성분을 분리하는 방법에 의한 것으로, 이러한 방법에 의해서는 사포닌, 진세노사이드 등의 인삼 중의 에탄올 가용부분만이 활용되었으며, 에탄올 불용부분은 폐기되었다. Panax ginseng CA meyer is a plant belonging to the genus Ogapi and ginseng. It has been used for more than 2,000 years in Korea, China, and Japan. It has been used for the purpose of preventing disease and extending the lifespan. It is said to have the effect of keeping the skin healthy by promoting it. In addition, the extract of ginseng has anti-complement activity, anti-cancer action, hypoglycemic action, anti-ulcer action and immune enhancing action has been reported recently through many studies. This study is mainly performed by extracting ginseng with an aqueous ethanol solution and then performing solvent fractionation to separate single components using silica gel column chromatography or C-18 column chromatography. Only the ethanol soluble portion of ginseng, such as ginsenoside, was used, and the ethanol insoluble portion was discarded.

그러나, 본원 발명에서는 기존에 폐기처리되던 에탄올 불용부분을 활용하여 인삼의 추출물이 피부의 노화방지에 효과적이라는 것을 발견한 것으로, 본원 발명에서 인삼 추출물은 인삼(Panax ginsengC.A. Meyer) 약 10kg에 95% 에탄올 약 100ℓ를 가하여 3시간동안 가열, 환류, 추출하여 에탄올 가용성분을 제거한 다음, 불용성분을 포함한 잔사를 다시 물 약 100ℓ로 3시간동안 가열, 환류, 추출하여 규조토로 여과한 후에 에탄올 약 250ℓ를 가하여 침전시킨 다음 이 침전물을 건조시킴으로써 얻어진다.However, the present invention was found that the extract of ginseng is effective to prevent the aging of the skin by utilizing the ethanol insoluble portion that was previously disposed of, ginseng extract in the present invention 95% to about 10kg ginseng ( Panax ginseng CA Meyer) After adding 100 l of ethanol, heating, refluxing and extracting for 3 hours to remove ethanol soluble components, the residue containing insoluble components was heated, refluxed and extracted with about 100 l of water for 3 hours, filtered through diatomaceous earth, and then about 250 l of ethanol. Precipitation by addition is followed by drying the precipitate.

본 발명의 피부 노화방지 화장료 조성물은 상기 인삼 추출물을 조성물 총 중량에 대하여 10-8∼101중량%, 바람직하게는 10-5∼5.0중량%의 양으로 함유된다.Skin anti-aging cosmetic composition of the present invention contains the ginseng extract in an amount of 10 -8 to 10 1 % by weight, preferably 10 -5 to 5.0% by weight based on the total weight of the composition.

본 발명의 피부 노화방지용 화장료 조성물은 그 제형에 있어서 특별히 한정되는 바가 없으며, 예를 들면, 유연화장수, 수렴화장수, 영양화장수, 영양크림, 마사지 크림, 에센스, 아이크림, 아이에센스, 클렌징크림, 클렌징폼, 클렌징워터, 팩, 파우더, 보디로션, 보디크림, 보디오일, 보디에센스 등의 화장료로 제형화될 수 있다.The anti-aging cosmetic composition of the present invention is not particularly limited in the formulation, for example, softening longevity, astringent longevity, nourishing longevity, nutrition cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing It can be formulated into cosmetics such as foams, cleansing water, packs, powders, body lotions, body creams, body oils, body essences and the like.

또한, 각 제형의 피부 노화방지용 화장료 조성물에 있어서, 상기한 인삼 추출물 이외의 성분들은 기타 화장료의 제형 또는 사용목적 등에 따라 당업자가 어려움없이 적의 선정하여 배합할 수 있다.In addition, in the cosmetic composition for preventing skin aging of each formulation, components other than the ginseng extract described above can be appropriately selected and blended by those skilled in the art without difficulty according to the formulation or purpose of use of other cosmetics.

이하, 본 발명의 시험예를 들어 본 발명의 구성 및 그 효과에 대하여 보다 구체적으로 설명한다.Hereinafter, the structure of the present invention and its effects will be described in more detail with reference to the test examples of the present invention.

〈참고예〉 인삼 추출물의 제조Reference Example Preparation of Ginseng Extract

인삼 10kg에 95% 에탄올 100ℓ를 가하여 3시간 동안 가열, 환류, 추출하여 에탄올 가용성분을 제거한 다음, 불용성분을 포함한 잔사를 다시 물 100ℓ로 3시간동안 가열, 환류, 추출하여 규조토로 여과한 후에 에탄올 약 250ℓ를 가하여 침전시킨 다음 이 침전물을 건조시켜 인삼 추출물을 얻었다.100 g of 95% ethanol was added to 10 kg of ginseng and heated, refluxed and extracted for 3 hours to remove soluble ethanol. The residue containing insoluble ingredients was then heated, refluxed and extracted with 100 liters of water for 3 hours and filtered through diatomaceous earth. About 250 L was added to precipitate, and the precipitate was dried to obtain a ginseng extract.

〈시험예 1〉섬유아세포(Fibroblast)의 증식효능 측정 Test Example 1 Measurement of Proliferation Effect of Fibroblasts

3.5%의 우태아 혈청이 함유된 DMEM(Dulbecco's Modified Eagle's Media)배지에서 배양한 인체 섬유아세포를 96공 평판배양기(96-well microtiter plate)에 5,000세포/well가 되도록 분주하고, 참고예의 인삼 추출물을 배양배지로 1/10씩 순차적으로 희석하여 첨가한 후, 37℃ 온도에서 4일간 배양하였다. 배양 후 0.2% MTT(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltertazolium bromide) 용액을 각 well당 50㎕씩 첨가하고, 다시 37℃ 온도에서 4시간동안 배양한 후 생성된 포르마잔(formazan)을 DMSO(Dimethyl sulfoxide)로 용해시켰다. 용해된 포르마잔의 흡광도를 평판배양측정기(microplate reader)를 이용하여 570nm에서 측정하였다. 이를 인삼 추출물을 처리하지 않은 대조군에 대하여 상기와 동일한 방법으로 실시하여 흡광도를 측정하였다. 인삼 추출물을 함유한 실험군과 대조군의 흡광도를 비교한 후, 그 결과를 표 1에 나타내었다.Human fibroblasts cultured in DMEM (Dulbecco's Modified Eagle's Media) medium containing 3.5% fetal bovine serum were dispensed to 5,000 cells / well in a 96-well microtiter plate, and the ginseng extract of Reference Example After diluting sequentially by 1/10 with the culture medium, the mixture was incubated at 37 ° C. for 4 days. After incubation, 50 μl of 0.2% MTT (3- [4,5-dimethylthiazol-2-yl] -2,5-diphenyltertazolium bromide) solution was added to each well, followed by incubation at 37 ° C. for 4 hours. Formazan was dissolved in dimethyl sulfoxide (DMSO). The absorbance of the dissolved formazan was measured at 570 nm using a microplate reader. This was carried out in the same manner as above for the control group not treated with ginseng extract was measured for absorbance. After comparing the absorbance of the experimental group and the control group containing the ginseng extract, the results are shown in Table 1.

시료농도(%)Sample concentration (%) 세포증식능(%)Cell proliferation (%) 1 × 10-8 1 × 10 -8 44 1 × 10-7 1 × 10 -7 77 1 × 10-6 1 × 10 -6 1010 1 × 10-5 1 × 10 -5 1515 1 × 10-4 1 × 10 -4 2323 1 × 10-3 1 × 10 -3 5050 1 × 10-2 1 × 10 -2 5757 1 × 10-1 1 × 10 -1 6565

상기 표 1에서 알 수 있는 바와 같이, 인삼 추출물을 처리하지 않은 섬유아세포에 비하여, 인삼 추출물을 처리한 섬유아세포는 최고 약 65% 정도 향상된 섬유아세포 증식 효능을 나타내었다.As can be seen in Table 1, the fibroblasts treated with ginseng extract showed up to about 65% improved fibroblast proliferation efficiency compared to the fibroblasts not treated with ginseng extract.

〈시험예 2〉각질형성세포(Keratinocyte)의 증식효능 측정 <Test Example 2> Measurement of proliferation effect of keratinocytes

각질형성세포를 사용하여 시험예 1에서와 동일한 방법으로 각질형성세포의 증식효능을 측정하여, 그 결과를 표 2에 나타내었다.Using the keratinocytes to measure the proliferation effect of the keratinocytes in the same manner as in Test Example 1, the results are shown in Table 2.

시료농도(%)Sample concentration (%) 세포증식능(%)Cell proliferation (%) 1 × 10-8 1 × 10 -8 44 1 × 10-7 1 × 10 -7 88 1 × 10-6 1 × 10 -6 99 1 × 10-5 1 × 10 -5 1313 1 × 10-4 1 × 10 -4 1515 1 × 10-3 1 × 10 -3 2424 1 × 10-2 1 × 10 -2 3030 1 × 10-1 1 × 10 -1 3333

상기 표 2에서 알 수 있는 바와 같이, 인삼 추출물을 처리하지 않은 각질형성세포에 비하여, 인삼 추출물을 처리한 각질형성세포는 최고 약 33% 정도 향상된 각질세포 증식 효능을 나타내었다.As can be seen in Table 2, compared to keratinocytes not treated with ginseng extract, keratinocytes treated with ginseng extract showed up to about 33% enhanced keratinocyte proliferation efficacy.

〈시험예 3〉섬유아세포의 콜라겐 합성정도의 측정 <Test Example 3> Measurement of collagen synthesis degree of fibroblasts

인체 섬유아세포를 24공 평판배양기에 배양한 후, 참고예의 인삼 추출물을 배양배지로 1/10씩 순차적으로 희석하여 첨가하였다. 배양 3일째 10%의 우태아 혈청이 함유된 DMEM배지를 각 well당 0.5㎖씩 첨가한 후 L[2, 3, 4, 5-3H]-프롤린 10μCi를 첨가하였다. 24시간 후 각 well에 들어있는 배지와 세포들을 긁어모아 5% 트리클로로아세틱엑씨드(TCA; Trichloroacetic acid) 용액에 넣어 수세한 후 2개의 시험관에 분주하고, 1개의 시험관에는 타입 I 콜라게나제(type I collagenase) 1unit/㎕를 넣고 37℃ 온도에서 90분간 배양하였으며, 다른 시험관은 4℃에서 보관하였다. 그후 모든 시험관에 50% TCA를 0.05㎖씩 첨가하고 4℃에서 20분간 방치한 후 각각 12,000rpm에서 10분간 원심분리하여 각각의 상등액과 침전물을 액체 신틸레이션 계수기(LSC; Liquid Scintillation Counter)로 디피엠(dpm; decay per minute) 값을 얻어 하기 수학식 1에 의거하여 인삼 추출물을 처리하지 않은 대조군과 실험군에 대한 콜라겐 생합성 값(RCB; Relative Collagen Biosynthesis)을 구하고, 그 결과를 하기 표 3에 나타내었다.After culturing human fibroblasts in a 24-hole plate incubator, the ginseng extract of the reference example was added by diluting sequentially by 1/10 with the culture medium. DMEM medium containing 10% fetal calf serum on day 3 of culture was added 0.5ml per well, and then 10 μCi of L [2, 3, 4, 5- 3 H] -proline was added. After 24 hours, the medium and cells in each well are scraped, washed in 5% Trichloroacetic acid (TCA) solution, and washed in two test tubes, and in one test tube, type I collagenase ( 1 unit / μl of type I collagenase) was added and incubated at 37 ° C. for 90 minutes, and other test tubes were stored at 4 ° C. Thereafter, 0.05 ml of 50% TCA was added to all test tubes, and the mixture was left at 4 ° C. for 20 minutes, followed by centrifugation at 12,000 rpm for 10 minutes, respectively. The supernatants and precipitates were decompressed with a liquid scintillation counter (LSC). dpm ; decay per minute) to obtain a collagen biosynthesis value ( RCB ; Relative Collagen Biosynthesis) for the control group and the experimental group not treated with ginseng extract based on Equation 1 below, the results are shown in Table 3 below.

시료농도(%)Sample concentration (%) 콜라겐 합성능(%)Collagen Synthesis (%) 1 × 10-8 1 × 10 -8 33 1 × 10-7 1 × 10 -7 33 1 × 10-6 1 × 10 -6 88 1 × 10-5 1 × 10 -5 1111 1 × 10-4 1 × 10 -4 1717 1 × 10-3 1 × 10 -3 2525 1 × 10-2 1 × 10 -2 2626 1 × 10-1 1 × 10 -1 3131

상기 표 3에서 알 수 있는 바와 같이, 인삼 추출물을 처리하지 않은 섬유아세포에 비하여, 인삼 추출물을 처리한 섬유아세포는 최고 약 31% 정도 향상된 섬유아세포의 콜라겐 생합성 촉진효능을 나타내었다.As can be seen in Table 3, the fibroblasts treated with ginseng extract showed enhanced collagen biosynthesis activity of fibroblasts up to about 31% compared to the fibroblasts not treated with ginseng extract.

〈시험예 4〉피부 자극시험 <Test Example 4> Skin irritation test

본원 발명의 인삼 추출물의 피부자극정도를 실험하기 위하여, 20∼30세의 건강한 여성 30명을 대상으로 CTFA 가이드라인(The Cosmetic, Toiletry and Fragrance Association. Inc. Washington, D.C., 20036, 1991)에 따라 실시하였다. 핀 챔버(Finn Chamber)에 준비된 시험물질(10% in Patch base) 20㎕를 적하시킨 후 70% 에탄올로 세척한 후 건조시킨 시험부위인 등 부위에 얹어 미소공테이프(micropore tape)로 고정시켰다. 첩포는 24시간동안 도포하며, 첩포를 제거한 후 마킹팬(marking pen)으로 시험 부위를 표시하며 30분, 24시간 후에 각 시험 부위의 피부반응을 관찰하여 국제 접촉 피부염 연구회(International Contact Dermatitis Research Group: ICDRG)의 규정에 따라 판정하였다. 하기 수학식 2를 이용하여 평균반응도를 계산한 후 그 결과를 하기 표 4에 나타내었다. 또한, 평균반응도를 기초로 하여 피부자극 정도를 다음의 4등급으로 분류하였다.In order to test the skin irritation degree of the ginseng extract of the present invention, according to CTFA guidelines (The Cosmetic, Toiletry and Fragrance Association. Inc. Washington, DC, 20036, 1991) in 30 healthy women aged 20 to 30 years Was carried out. 20 μl of the test substance (10% in Patch base) prepared in the Finn Chamber was added dropwise, washed with 70% ethanol, and placed on the back of the dried test site and fixed with a micropore tape. The patch is applied for 24 hours. After removing the patch, mark the test site with a marking pen. After 30 minutes and 24 hours, the skin reaction of each test site is observed. The International Contact Dermatitis Research Group: ICDRG). After calculating the average reactivity using Equation 2 shown in Table 4 below. Also, based on the average reactivity, the degree of skin irritation was classified into the following four grades.

·피부자극 판정 기준 :Skin irritation determination criteria:

Ⅰ등급 …… MS<1(무자극)Ⅱ등급 …… 1≤MS<3(경자극)Class I… … MS <1 (non-irritating) II grade… … 1≤MS <3 (light stimulus)

Ⅲ등급 …… 3≤MS<5(중자극)Ⅳ등급 …… MS≥5(강자극)Class III… … 3 ≤ MS <5 (medium stimulation) IV grade. … MS≥5 (strong stimulus)

시험물질Test substance 24시간24 hours 48시간48 hours 평균반응도(n=30)Average reactivity (n = 30) 판정등급Judgment grade ++ ++ ++++ ++++++ ±± ++ ++++ ++++++ 인삼 추출물(농도: 10% 염수)Ginseng Extract (Concentration: 10% Saline) 1One ­­ ­­ ­­ ­­ ­­ ­­ ­­ 0.420.42 I 인삼 추물물(농도: 1% 염수)Ginseng Extract (Concentration: 1% brine) 33 ­­ ­­ ­­ ­­ ­­ ­­ ­­ 1.251.25

상기 표 4에서 알 수 있는 바와 같이, 본 발명의 인삼추출물은 피부자극이 거의 없슴을 확인할 수 있었다.As can be seen in Table 4, the ginseng extract of the present invention was confirmed that almost no skin irritation.

이상의 시험예에서 알 수 있는 바와 같이, 인삼추출물은 피부자극이 없으면서 피부세포 증식 및 콜라겐의 생합성을 촉진하는 물질임을 알 수 있다.As can be seen in the above test example, it can be seen that ginseng extract is a substance that promotes skin cell proliferation and collagen biosynthesis without skin irritation.

〈시험예 4〉인체피부를 대상으로 한 피부주름 개선효과 <Test Example 4> Skin wrinkle improvement effect on human skin

35∼45세의 안면주름이 있는 시험대상자 30명에 대하여, 하기 표 4와 같은 조성을 갖는 영양크림에 대하여 인삼 추출물을 함유한 실시예 1과 인삼 추출물을 함유하지 않은 비교예 1의 피부주름 개선효과를 비교평가하게 하였다.Skin wrinkle improvement effect of Example 1 containing ginseng extract and Comparative Example 1 containing no ginseng extract with respect to the nourishing cream having the composition shown in Table 4 for 30 subjects with facial wrinkles aged 35 to 45 years Was evaluated.

성 분ingredient 단위 : 중량%Unit: weight% 제형예 1Formulation Example 1 비교예 1Comparative Example 1 인삼 추출물Ginseng extract 0.10.1 -- 밀납Beeswax 10.010.0 10.010.0 폴리솔베이트 60Polysorbate 60 1.51.5 1.51.5 피이지 60 경화피마자유Sebum 60 Cured Castor Oil 2.02.0 2.02.0 솔비탄세스퀴올레이트Sorbitan sesquioleate 0.50.5 0.50.5 유동파라핀Liquid paraffin 10.010.0 10.010.0 스쿠알란Squalane 5.05.0 5.05.0 카프릴릭/카프릭트리글리세라이드Caprylic / Capric Triglycerides 5.05.0 5.05.0 글리세린glycerin 5.05.0 5.05.0 부틸렌글리콜Butylene glycol 3.03.0 3.03.0 프로필렌글리콜Propylene glycol 3.03.0 3.03.0 트리에탄올아민Triethanolamine 0.20.2 0.20.2 방부제, 색소, 향료Preservative, coloring, flavoring 적량Quantity 적량Quantity 정제수Purified water to 100to 100 to 100to 100

피검자의 안면 좌부에는 제형예 1을, 우부에는 비교예 1을 3개월간 사용하도록 하였다. 크림사용 이전의 안면 양쪽부의 피부상태를 측정해 놓은 후 크림사용 3개월 후 동일부위를 재측정하여 피부주름의 변화를 측정하였다. 피부측정은 24℃ 온도, 상대습도 40%의 항온항습실에서 하였으며, 눈꼬리부위의 주름을 레플리카(replica)로 떠서 비시오메타 시스템(Visiometer system; C+K사)으로 피부주름을 측정하였다. 피부주름의 변화량은 하기 수학식 3에 따라 계산하였다.Formulation example 1 was used for the facial left side of the subject, and comparative example 1 was used for the right side for 3 months. The skin condition was measured on both sides of the face before the use of the cream, and three months after the use of the cream, the same area was remeasured to measure the change in skin wrinkles. Skin measurement was performed in a constant temperature and humidity room with a temperature of 24 ° C. and a relative humidity of 40%. The wrinkles at the tail of the eyes were replicated, and skin wrinkles were measured using a Visiometer system (C + K). The amount of change in skin wrinkles was calculated according to Equation 3 below.

(상기 식에서,(Wherein

Tdi; D90에서의 측정부위 값이며,Tdo; D0에서의 측정부위 값이다) Tdi ; Measured value at D 90 , Tdo ; Measured value at D 0 )

상기 식에 따라 계산한 결과, 비교예 1을 사용한 부위의 피부주름은 8±5%(평균±표준편차)의 감소치를 나타낸 반면, 실시예 1을 사용한 부위의 피부주름은32±13%의 감소치를 보여 우수한 피부주름 개선효과를 나타내었다.As a result of calculation according to the above formula, the skin wrinkle at the site using Comparative Example 1 showed a decrease of 8 ± 5% (mean ± standard deviation), whereas the skin wrinkle at the site using Example 1 was reduced to 32 ± 13%. To improve skin wrinkles.

이하, 상기한 시험예들의 결과를 근거로 하여, 인삼 추출물을 함유함으로써 피부 노화방지 효과를 제공할 수 있는 여러 제형의 화장료를 조성하여 제시한다. 그러나 본 발명의 조성물이 하기의 제형예에 한정되는 것은 아니다.Hereinafter, based on the results of the test examples described above, by presenting a cosmetic composition of various formulations that can provide a skin anti-aging effect by containing ginseng extract. However, the composition of the present invention is not limited to the following formulation example.

(제형예 2) 유연화장수(스킨로션)Formulation Example 2 Softening Cosmetic (Skin Lotion)

배합성분Ingredient 중량%weight% 인삼 추출물Ginseng extract 0.10.1 글리세린glycerin 3.03.0 부틸렌글리콜Butylene glycol 2.02.0 프로필렌글리콜Propylene glycol 2.02.0 카르복시비닐폴리머Carboxy Vinyl Polymer 0.10.1 피이지-12 노닐페닐에테르Fiji-12 nonylphenyl ether 0.20.2 폴리솔베이트 80Polysorbate 80 0.40.4 에탄올ethanol 10.010.0 트리에탄올아민Triethanolamine 0.10.1 방부제, 색소, 향료Preservative, coloring, flavoring 적량Quantity 정제수Purified water to 100to 100

(제형예 3) 영양화장수(밀크로션)Formulation Example 3 Nutrients (Milk Lotion)

배합성분Ingredient 중량%weight% 인삼 추출물Ginseng extract 0.50.5 스쿠알란Squalane 5.05.0 밀납Beeswax 4.04.0 폴리솔베이트 60Polysorbate 60 1.51.5 솔비탄세스퀴올레이트Sorbitan sesquioleate 1.51.5 유동파라핀Liquid paraffin 0.50.5 카프릴릭/카프릭트리글리세라이드Caprylic / Capric Triglycerides 5.05.0 글리세린glycerin 3.03.0 부틸렌글리콜Butylene glycol 3.03.0 프로필렌글리콜Propylene glycol 3.03.0 카르복시비닐폴리머Carboxy Vinyl Polymer 0.10.1 트리에탄올아민Triethanolamine 0.20.2 방부제, 색소, 향료Preservative, coloring, flavoring 적량Quantity 정제수Purified water to 100to 100

(제형예 4) 영양크림Formulation Example 4 Nutrition Cream

배합성분Ingredient 중량%weight% 인삼 추출물Ginseng extract 2.02.0 밀납Beeswax 10.010.0 폴리솔베이트 60Polysorbate 60 1.51.5 피이지 60 경화피마자유Sebum 60 Cured Castor Oil 2.02.0 솔비탄세스퀴올레이트Sorbitan sesquioleate 0.50.5 유동파라핀Liquid paraffin 10.010.0 스쿠알란Squalane 5.05.0 카프릴릭/카프릭트리글리세라이드Caprylic / Capric Triglycerides 5.05.0 글리세린glycerin 5.05.0 부틸렌글리콜Butylene glycol 3.03.0 프로필렌글리콜Propylene glycol 3.03.0 트리에탄올아민Triethanolamine 0.20.2 방부제, 색소, 향료Preservative, coloring, flavoring 적량Quantity 정제수Purified water to 100to 100

(제형예 5) 마사지크림Formulation Example 5 Massage Cream

배합성분Ingredient 중량%weight% 인삼 추출물Ginseng extract 5.05.0 밀납Beeswax 10.010.0 폴리솔베이트 60Polysorbate 60 1.51.5 피이지 60 경화피마자유Sebum 60 Cured Castor Oil 2.02.0 솔비탄세스퀴올레이트Sorbitan sesquioleate 0.80.8 유동파라핀Liquid paraffin 40.040.0 스쿠알란Squalane 5.05.0 카프릴릭/카프릭트리글리세라이드Caprylic / Capric Triglycerides 4.04.0 글리세린glycerin 5.05.0 부틸렌글리콜Butylene glycol 3.03.0 프로필렌글리콜Propylene glycol 3.03.0 트리에탄올아민Triethanolamine 0.20.2 방부제, 색소, 향료Preservative, coloring, flavoring 적량Quantity 정제수Purified water to 100to 100

(제형예 6) 팩(Formulation Example 6) Pack

배합성분Ingredient 중량%weight% 인삼 추출물Ginseng extract 5.05.0 폴리비닐알콜Polyvinyl alcohol 13.013.0 소듐카르복시메틸셀룰로오스Sodium Carboxymethyl Cellulose 0.20.2 글리세린glycerin 5.05.0 알란토인Allantoin 0.10.1 에탄올ethanol 6.06.0 핑지-12 노닐페닐에테르Pingji-12 Nonylphenyl Ether 0.30.3 폴리솔베이트 60Polysorbate 60 0.30.3 방부제, 색소, 향료Preservative, coloring, flavoring 적량Quantity 정제수Purified water to 100to 100

(제형예 7) 젤Formulation Example 7 Gel

배합성분Ingredient 중량%weight% 인삼 추출물Ginseng extract 1.01.0 에틸렌디아민초산나트륨Ethylenediamine Sodium Acetate 0.050.05 글리세린glycerin 5.05.0 카르복시비닐폴리머Carboxy Vinyl Polymer 0.30.3 에탄올ethanol 5.05.0 피이지-60 경화피마자유Fiji-60 Cured Castor Oil 0.50.5 트리에탄올아민Triethanolamine 0.30.3 방부제, 색소, 향료Preservative, coloring, flavoring 적량Quantity 정제수Purified water to 100to 100

이상에서 설명한 바와 같이, 피부세포 증식 및 콜라겐 생합성 촉진효과를 갖는 인삼 추출물을 화장료 조성물에 함유시킴으로써, 피부 노화방지에 효과적인 화장료 조성물을 제공할 수 있다.As described above, by containing the ginseng extract having a skin cell proliferation and collagen biosynthesis promoting effect in the cosmetic composition, it is possible to provide a cosmetic composition effective for preventing skin aging.

Claims (1)

피부 노화방지용 화장료 조성물에 있어서, 인삼(Panax ginsengC.A. Meyer)의 에탄올 추출 후 에탄올 불용잔사를 열수추출하여 규조토로 여과한 후, 에탄올을 가하여 침전시켜 얻은 인삼다당체를 조성물 총 중량에 대하여 10-8∼101중량%의 양으로 함유함을 특징으로 하는 피부 노화방지용 화장료 조성물.In the anti-aging cosmetic composition, ginseng ( Panax ginseng C.A. Meyer) is extracted with ethanol, hot water extracted from ethanol, filtered through diatomaceous earth, and then precipitated by adding ethanol to the ginseng polysaccharide obtained from 10 -8 to the total weight of the composition. A skin anti-aging cosmetic composition, characterized in that contained in an amount of 10 1 % by weight.
KR1019980019526A 1998-05-28 1998-05-28 Anti-aging cosmetic compositions containing an Extract obtained from Panax ginseng KR100361433B1 (en)

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WO2010123246A2 (en) * 2009-04-21 2010-10-28 (주)아모레퍼시픽 Cosmetic composition containing white ginseng powder
KR20240120405A (en) 2023-01-31 2024-08-07 우석대학교 산학협력단 Cosmetic composition comprising extract of above-ground part of ginseng

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KR100920961B1 (en) * 2002-06-25 2009-10-09 가부시키가이샤 시세이도 Anti-aging agent
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WO2009151302A2 (en) 2008-06-13 2009-12-17 주식회사 운화바이오텍 Composition for anti-aging or antioxidation containing plant stem cell lines derived from cambium of panax ginseng including wild ginseng and ginseng as active components
US9095532B2 (en) 2008-06-13 2015-08-04 Unhwa Corporation Composition for anti-aging or antioxidant composition containing plant stem cell line derived from cambium or Panax ginseng including wild ginseng and ginseng as active components
WO2010123246A2 (en) * 2009-04-21 2010-10-28 (주)아모레퍼시픽 Cosmetic composition containing white ginseng powder
WO2010123246A3 (en) * 2009-04-21 2011-03-24 (주)아모레퍼시픽 Cosmetic composition containing white ginseng powder
KR20240120405A (en) 2023-01-31 2024-08-07 우석대학교 산학협력단 Cosmetic composition comprising extract of above-ground part of ginseng

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