KR20110117876A - Cosmetic composition comprising flower and tea fermentation extract having anti-oxidant and anti inflammatory effect - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a fermented tea extract having an antioxidant and anti-inflammatory effect, comprising a fermented extract extracted by fermenting green tea, goji berry, jujube, persimmon leaf, gold, chrysanthemum and azalea with yeast or lactic acid bacteria as an active ingredient. It relates to a cosmetic composition characterized in that.
The present invention can be usefully used as a cosmetic composition having an antioxidant and anti-inflammatory effect such as free radical scavenging, lipoxygenase activity inhibition, hyaluronidase activity inhibition, and the like.

Description

Cosmetic composition containing fermented tea extract having antioxidant and anti-inflammatory effect {COSMETIC COMPOSITION COMPRISING FLOWER AND TEA FERMENTATION EXTRACT HAVING ANTI-OXIDANT AND ANTI INFLAMMATORY EFFECT}

The present invention relates to a cosmetic composition containing a tea fermentation extract having an antioxidant and anti-inflammatory effect, and more particularly, by inhibiting inflammation or other radical reactions caused by hyaluronidase, lipoxinase, free radicals, etc. It relates to a cosmetic composition that is effective.

One of the representative causes of skin trouble is free radicals present in the human body, which destroys cells, cuts connective tissue of the skin dermis layer or causes crosslinking. As a result, skin wrinkles, skin cancer, cell death, rheumatoid arthritis, atopic dermatitis, acne, and various problems occur.

These free radicals are produced by the phagocytosis of leukocytes, the electron transport system during metabolism of ATP in mitochondria, metabolism, stress, pollutants, and bacteria. Of course, the human body includes antioxidants (SuperOxide Dismatase (SOD), catalase, vitamin E, vitamin C, ubiquinol, etc.). Broken by stress or the like, free radicals gradually increase.

Another major cause of skin problems is the inflammatory response. Inflammation is a physiological response that protects the body from harmful environmental conditions, such as invasion and mechanical damage of foreign objects such as bacteria.

These inflammations increase many types of polymorphonuclear leukocytes (PMNs) and immune substances, and these cells treat and defend by releasing various types of proteolytic enzymes and cytokines, which are inflammatory cell products.

In particular, enzymes such as elastase, hyaluronidase, and lipoxygenase break down proteins and lipids produced during inflammation, but their actions are sometimes contiguous to tissue cells and non-cellular components. It can also cause harmful damage.

Thus, inflammation is restored to normal function after an initial condition under certain conditions, but if the irritating stimulant is not eliminated or made continuously, chronic inflammation occurs and causes more serious tissue damage. As a result, cells and connective tissues are damaged by proteolytic enzymes caused by excessive inflammation, and the damage of these connective tissues reduces the elasticity of the skin and causes wrinkles, as well as regeneration and proliferation of cells. It will have a bad effect, leading to rapid skin aging.

Therefore, in order to improve skin troubles caused by free radicals and inflammation, it is essential to develop a material having no side effects and low cost, and to develop an easy-to-use cosmetic containing the material.

To date, materials commonly used for antioxidant, anti-inflammatory and atopic skin improvement are known to have free radical scavenging effects such as ascorbic acid and alpha-tocophenol. However, these are not only expensive, but also have a problem in that it is difficult to obtain a practical effect because of poor stability during formulation. Therefore, a free radical scavenging effect, skin improvement effect, low production cost, and easy to use cosmetic composition is required.

Accordingly, various kinds of cosmetic compositions based on natural materials which are easy to purchase and useful for skin have been proposed.

For example, the tea tree (Camellia sinensis) Is a evergreen evergreen tree with thick, long oval leaves. White flowers bloom in autumn. Fruits are flat and rounded with three corners. According to the processing method of tea leaves, green tea, black tea, flower tea (black tea) is divided into, and the leaves have 1-6% purine bases such as caffeine, xanthine and theophylline. Tea tree tannin is 9-15%, of which soluble tannin is about 3% and insoluble tannin is 9.9%. Green tea contains about 0.006% of essential oils, and alkaloids include caffeine, theophylline, theobromine, xanthine, adenine, coumarin, vitamins B1, B2, B3, B5, and K. Green tea is dried immediately without picking the young leaves of the tea tree, and the fragrance is weak but physiologically more active. Since green tea has antimicrobial activity, it is used as a decoction for the treatment of thyme (components and use of herbs, Encyclopedia of Science, January, p. 191).

Republic of Korea Patent Publication No. 2004-0094513 refers to a cosmetic composition comprising vitamin C and one or more herbal extracts selected from the group consisting of green tea, ginjin mugwort and pine needles, mentioning that these extracts can be used as a natural functional cosmetic composition.

Wolfberry ( Lycium chinensis ) is a weeping or egg-shaped leaf alternately attached, branched flowers bloom on the leaves and oval fruits ripen reddish-green. It is called goji because the thorns of this plant are like the lyrics of the tree (old) and the stem is similar to the wicker (gi). The fruit contains 0.1% of vitamin and zeaxanthin, a carotenoid, and fisaliene from the fruit peel and scopoline from the fruit. In addition, carotenoids and sterols were also isolated, which are pisalien and beta-sitosterol. 1.5% of diosgenin is present on the branch with leaves, betaine, doucosterol, and ascorbic acid are present on the leaves, and betaine is on the root bark (medicinal ingredients and uses of herbs, scientific encyclopedia publishers, January Worth, 1991).

Republic of Korea Patent Publication No. 2006-82205 is a whitening cosmetic composition comprising the extract of Goji, Shinyi (Magnolia bud), Republic of Korea Patent Registration No. 10-0753186 is made of sewage, Baekbokyeong, dew, donkey, gojija, earth and sand and ginseng It proposes a cosmetic composition for preventing skin aging, comprising a herbal extract mixture as an active ingredient.

The leaves of the jujube tree ( Zizyphus jujuba ) are egg-shaped and glossy. In early summer light pale flowers bloom, the fruit is long oval and ripens red chestnut. There are 20-49% dry matter in the fruit of the jujube tree, of which 14-42% (up to 65% for dried fruits) is sugar, mainly composed of fructose, sucrose, glucose and rhamnose. ˜35% is fructose, and organic acids such as cinnamic acid, malic acid, grape acid, succinic acid, and glycolic acid occupy 0.54 to 3.4% (there are 1-7% organic acids in jujube fruits). Fruits contain 1.9-2.2% of protein and 14 amino acids, including cystine, lysine, arginine, aspartic acid, threonine, alanine, tyrosine, aminofatty acid, proline, methionine, valine, phenylalanine, leucine and isoleucine. The leaves also contain 9% tannins, 3.7% flavonoids, coumarins, saponins, sugars, uronic acids, resins, essential oils, ascorbic acid, carotene, folic acid, and vitamin B1. Publishing House, January Wolken, 1991).

Republic of Korea Patent Publication No. 2005-24831 refers to a cosmetic composition having an anti-inflammatory effect, containing the extract of fruit root, jujube and yellow white as an active ingredient, Patent Publication No. 2005-97578 contains jujube extract and walnut extract The cosmetic composition for moisturizing the skin, Patent Publication No. 2008-0094457, Licorice, Gobon, Gwakyang, Gilkyung, Deer Antler, Angelica, Jujube, Cordyceps sinensis, Mokhyang, Baekbokyeong, Baekji, Cod, Muskcho, Pomegranate, Seokpopo, Truffle mushroom, Contains fermented extract of medicinal herb selected from the group consisting of pine, ganoderma, oak, gentian, ginseng, peony, cloves, sculptor, dermis, creation, cheonsan snow softening, cheonhwa flour, cactus, almond and red ginseng as active ingredients A cosmetic composition for improving wrinkles is proposed.

Persimmon tree ( Diospyros kaki ) leaves are wide oval or oval and stick out. Female and male flowers bloom separately on one tree, and the fruit is ripe in black or yellow. Persimmon trees are planted in saplings and planted with elderberry to obtain persimmon trees that can withstand the cold. There are many ascorbic acids in the leaves, and up to 1,200mg in young leaves, but as the leaves grow, they are about 300mg in large leaves. There are also vitamins P, B1, B2 and K, essential oils, organic acids, carotene, leuco delphinidin and its glycosides, camphorol-3-glucoside (0.02%), myricithin (0.15%) and iodine. According to the component analysis data of tea made with leaves, water 3.2%, protein 14.4%, lipid 6.9%, sugar 54.5%, fiber 11.6%, ash 9.4%, tannin 4.2%, caffeine 0.3%, choline 480 mg, ascorbic acid It is known that 634 mg. Fruit stems include oleanolic acid, ursolic acid, betulinic acid, triterpenecarboxylic acid, glucose, fructose, tannins, organic acids (lemonic acid, malic acid), etc. 1991).

Korean Patent Registration No. 10-0820009 proposes an antioxidant and skin soothing herbal composition containing persimmon leaf, licorice, baeknyeoncho, and baekbaekpi, and patent registration No. 10-0898182 describes aloe, eoseongcho, domestic country, persimmon leaf, dandelion, It is mentioned that a cosmetic composition comprising an active ingredient of a fermentation broth fermenting plants containing Angelica gigas is effective for atopic dermatitis.

The leaves of the honeysuckle ( Lonicera japonica ) face each other in the shape of a long egg. Two long tubular flowers bloom on the leaves. Flowers are called gold and silver because they are initially white and gradually turn yellow. It is said to be endurable even in winter. The flowers include luteolin and inositol, and the flowers are sweet because of inositol. The leaves and stems contain 5-8% tannins, and the leaves contain loganine, about 19% nitrogenous substances, and luteolin-7, a luteolin-7-rhamnoglucoside. Iridoid compounds ronisroid and sweroside were isolated from the leaves of the same genus plant. Flowering decoctions of honeysuckle are also effective against erythropoies, typhoid bacteria, staphylococci, and pneumococcal pneumoniae (components and uses of herbs, Encyclopedia of Science, January, 1991).

Republic of Korea Patent Registration No. 10-0889606 is a term comprising a fermented extract extracted by fermenting mushroom fungus fermented with a yeast, lactic acid bacteria or bifidus bacteria of at least one selected from the group consisting of Cheonggung, Geumgumhwa, jihwang, hyeonggae and Astragalus The present invention proposes an inflammatory cosmetic composition, and Patent Publication No. 2008-0011546 proposes a hypoallergenic skin external preparation composition containing a sterling silver extract to remove the irritation caused by chemical preservatives and to maintain the preservative power while maintaining skin preservation.

Chrysanthemum morifolium ) flowers and stems contain essential oils and also contain adenine and choline. Flowers contain chrysanthemin (cyanidin-3-glucoside), amino acids, flavonoids and vitamin B1. Essential oils contain mainly borneol, camphor, chrysanthenone and the like, and flavonoids such as luteolin-7-glucoside, cosmosiin and acacin ( acaciin), including pure dimethyl hydrazide (aminozide) (Ilbohyang medicinal herb metabolism, Jung Byun, Shin Mingyo, Younglimsa, 1990).

Korean Patent Laid-Open Publication No. 2009-17282 discloses herbal extracts containing antioxidant activity, that is, oriental medicine including Angelica, Peony, Peony, Red Bokyong, Yongan, Safflower, Hyangbuja, Gardenia, Peppermint Leaf, Shiho, Chrysanthemum, Jujube and Honey It refers to a cosmetic composition containing a complex herbal extract, Patent Publication No. 2009-26826 discloses a plant consisting of persimmon leaves, cinnamon, chrysanthemum, Chinese quince, green tea, green mushrooms, ganoderma lucidum mushroom, matsutake mushrooms, vitamin C and vitamin E It is disclosed that the plant extract obtained by ultrasonic extraction together has an excellent antioxidant effect.

Rhododendron mucronulatum ) The leaves are oval and alternately attached, the flowers bloom in violet red before the leaves sprout in early spring. The leaves include andromedotoxin, azaleatin, azalein, abicurin, hyperpherides, quercetin, tannins, essential oils, ursolic acid, vanillic acid, and ciringaic acid, and there are also andromedotoxins in flowers and branches. In addition, the andromedotoxin content of the leaves is high in early spring and greatly lowered from summer to autumn (components and use of herbs, Encyclopedia of Science, January, 1991).

Korean Patent Registration No. 10-0776695 discloses a cosmetic composition for improving atopic dermatitis treatment comprising azalea extract as an active ingredient, and Patent Publication No. 2008-57414 alleviates skin irritation comprising azalea root extract as an active ingredient Patent No. 10-0829722 discloses a cosmetic composition for preventing skin aging and a cosmetic composition for alleviating skin inflammation comprising a root extract as an active ingredient.

Korean Patent Publication No. 2004-94513; Korean Patent Publication No. 2006-82205; Korean Patent Registration No. 10-0753186; Korean Patent Publication No. 2005-24831; Korean Patent Publication No. 2005-97578; Korean Patent Publication No. 2008-94457; Republic of Korea Patent Registration No. 10-0820009; Korean Patent Registration No. 10-0898182; Korean Patent Registration No. 10-0889606; Korean Patent Publication No. 2008-11546; Korean Patent Publication No. 2009-17282; Korean Patent Publication No. 2009-26826; Korean Patent Registration No. 10-0776695; Korean Patent Publication No. 2008-57414; Republic of Korea Patent Registration No. 10-0829722

Herbal Use and Use, Encyclopaedia Scientific Publication, Ilwol Seok, 1991; Dohae Hyangje (Medicinal Herb), Korean Dictionary

The present inventors made various efforts to prepare a cosmetic composition having natural ingredients as a raw material, which is easy to purchase and low in production cost, and has antioxidant and anti-inflammatory activity, and selects specific teas and flowers, and tea fermentation extracts from them. To obtain a petal fermented extract and when used as a cosmetic composition to find out that the antioxidant and anti-inflammatory effect has a useful effect in improving the skin to complete the present invention.

Accordingly, an object of the present invention is to provide a cosmetic composition having antioxidant and anti-inflammatory activity.

In order to achieve the above object,

Tea fermented extracts obtained from green tea, persimmon leaves, jujube, and wolfberry; And

It provides a cosmetic composition comprising an anti-inflammatory and antioxidant effect comprising fermented tea extract consisting of petal fermented extract obtained from gold silver, chrysanthemum, and azalea as an active ingredient.

The composition containing the tea fermentation extract of the present invention has an effect such as anti-inflammatory and antioxidant, and can be applied as a cosmetic composition for skin improvement.

The present invention provides a cosmetic composition excellent in anti-inflammatory and antioxidant effects. As a raw material of the active ingredient of the cosmetic composition, a specific tea and petals known as natural ingredients are selected, each extract is obtained from them, and the mixing ratio between them is optimized to maximize the anti-inflammatory and antioxidant effects.

Tea fermentation extract according to the present invention is composed of tea fermentation extract and petal fermentation extract, the tea fermentation extract comprises green tea extract, persimmon leaf extract, jujube extract and wolfberry extract, the petal fermentation extract is a sterling gold extract, chrysanthemum extract and azalea Contains extracts.

These tea fermentation extracts include both tea fermentation extracts and petal fermentation extracts, which have the advantage that the anti-inflammatory and antioxidant effects are further improved while maintaining the respective effects obtained from the tea fermentation extracts and the petal fermentation extracts. In particular, the tea fermentation extract and the petal fermentation extract is 1: 9 to 9: 1 by weight, preferably 7: 3 to 9: 1 by weight, most preferably 7: 3 ratio of the maximum ratio of the effect is further maximized can do.

Preferably, the fermented tea extract is a mixture of green tea: persimmon leaf: jujube: goji berry extract in a weight ratio of 1-5: 1-5: 1-5: 1-5. This content ratio is obtained in consideration of the effects each component exhibits, in particular anti-inflammatory and antioxidant effects. If the content is less than the above range, the efficacy is insufficient, and if it exceeds the above range, the problem of discoloration and odor, the oxidized by-product. As a result, problems such as sedimentation or irritation may occur.

In addition, the petal fermentation extract uses what mixed gold, silver, chrysanthemum, and azalea extract in the weight ratio of 1-5: 1-5: 1-10. This content ratio is obtained in consideration of the effects of each component, anti-inflammatory and antioxidant, if the content is less than the above range, the efficacy is insufficient, on the contrary, if it exceeds the above range, the problem of discoloration and odor, the product of oxidized by-products Problems such as the occurrence of precipitation phenomenon and providing the cause of irritation occur.

Tea fermentation extract according to the present invention

Fermented green tea, wolfberry, jujube, persimmon leaf, gold and silver, chrysanthemum, and azalea with yeast or lactic acid bacteria to obtain fermentation broth,

It is obtained by extracting the fermentation component in the fermentation broth.

Green tea, goji berry, jujube, persimmon leaf, gold and silver chrysanthemum, and azalea used as raw materials can be purchased on the market, or directly extracted from wild acids or fields according to the conventional methods known in the art. Obtained.

The fermentation process is inoculated with distilled water , yeast or lactic acid bacteria in green tea, wolfberry, jujube, persimmon leaves, gold and silver chrysanthemum, azalea and basal medium (YM medium or MRS medium) (concentration about 10 ^{5-10 7} cells / ml), pH 5 Incubate for 24 to 120 hours while maintaining the conditions of -8 and a temperature of 20 to 45 ° C. After centrifugation to remove the precipitate and yeast cells or lactic acid bacteria.

During fermentation, the solvent is a basic medium for feeding yeast or lactic acid bacteria and distilled water.

The yeast is yeast is Saccharomyces ( Saccharomyces) cerevisiae ), Saccharomyces boulardii ), Saccharomyces ellipsoids ) and ski surveys- Schzosaccharomyces ellipsoids ) may be used from the group consisting of one.

In addition, Lactobacillus Lactobacillus ( Lactobacillus) acidophilus), Lactobacillus casei (Lactobacillus casei), Lactobacillus Bulgaria kusu (Lactobacillus bulgaricus ), Lactobacillus rhamnosus ), Lactobacillus plantarum ), Lactobacillus fermentum ), Lactobacillus brevis ), Lactobacillus 1 species is used from the group which consists of reuteri ) and Lactobacillus confusus .

These extracts are extracted after fermentation to increase the skin absorption by the production of new active ingredients and low molecular weight of the active ingredient, which is more effective than the extracts.

Usable extraction methods can be prepared by methods such as hot water extraction, room temperature extraction, warm extraction, ultrasonic extraction, supercritical extraction commonly used in this field.

Tea fermentation extract of the present invention is prepared by known extraction methods known to those skilled in the art. For example, in the tea fermentation extract of the present invention, the fermentation broth obtained through fermentation is used as it is, or after drying the fermentation broth, 1 to 20 times (volume) of the extraction weight is added to 3 to 3 to 4 to 30 ° C. 20 days of immersion to extract the active ingredient.

As the extraction solvent, one selected from the group consisting of water, lower glycols having 1 to 6 carbon atoms, lower alcohols having 1 to 4 carbon atoms, ethyl acetate, glycerin, and mixed solvents thereof is used.

Subsequently, the extraction solvent is concentrated in a vacuum concentrator, then dried to obtain a solid state, pulverized and mixed, and extracted using an extraction solvent having a volume of 1 to 20 times its dry weight. At this time, in order to prevent the solvent from evaporating, the extract with a cooling condenser is heated and extracted for 3 to 48 hours at 30 to 100 ° C. or deposited for 1 to 5 days at 5 to 30 ° C. to extract the active ingredient, and the extraction solvent is decompressed. Obtained by concentration with a thickener.

The extraction solvent uses a solvent having the same or similar properties as the above-described extraction solvent.

In addition, it can be prepared using a known ultrasonic extraction method, etc., extracted for 1 to 12 hours with an ultrasonic extractor in the extraction solvent at room temperature. In this case, the time of the ultrasonic extraction may vary depending on the amount of the sample to be extracted, and may undergo a process of filtering or purifying the extraction result.

The fermented tea extract as described above has anti-oxidant and anti-inflammatory effects at the same time and can be applied to various fields, and preferably can be used as a raw material of cosmetics.

In the composition of the present invention, the content of the tea fermentation extract is 0.001 to 10.0% by weight, preferably 0.01 to 5.0% by weight, more preferably 0.1 to 3% by weight based on the total weight of the cosmetic composition. If the content of the tea fermentation extract is less than the above range, there is a problem in showing the effect, on the contrary, in the case of exceeding the above range, there is no apparent increase in effect due to the increase in content, and the stability of the formulation is lowered.

The cosmetic composition of the present invention may be prepared in any formulation as is known, and may be a lotion, nourishing lotion, nourishing cream, massage cream, essence, pack, solution, suspension, emulsion, paste, gel, cream, lotion, powder, Soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, sprays and the like.

In addition, in the cosmetic composition of each formulation, other components in addition to the above tea fermentation extract may be arbitrarily selected and blended according to the formulation or purpose of use of other cosmetics. In addition, the compositions of each formulation may contain various bases and additives necessary and appropriate for the formulation of the formulation, and nonionic surfactants, silicone polymers, extender pigments, fragrances, preservatives, and fungicides within the scope of not impairing the effect thereof. , Oxidative stabilizers, organic solvents, ionic or nonionic thickeners, softeners, antioxidants, free radical destroyers, opacifiers, stabilizers, emollients, silicones, α-hydroxy acids, antifoams, humectants, vitamins And known compounds such as insect repellents, fragrances, preservatives, surfactants, anti-inflammatory agents, substance P antagonists, fillers, polymers, propellants, basicizing or acidifying agents, or colorants.

According to the preferred Experimental Examples 1 to 4 of the present invention, the cosmetic composition of the present invention confirmed the effects such as free radical scavenging, inhibition of lipoxygenase activity, inhibition of hyaluronidase activity, etc. It can be seen that the cosmetic composition for the purpose of improvement can be preferably applied.

In particular, it was confirmed that the mixed fermented extract according to the present invention exhibited a higher activity inhibitory effect compared to quercetin, which has conventional antioxidant and lipoxygenase activity inhibition (Experimental Examples 2 and 3). Although quercetin has various physiological activity effects, quercetin, which is almost insoluble in water, must use an excessive amount of a nonionic surfactant or a non-aqueous solvent in order to increase solubility, which has a safety problem of occurrence of irritation. By using the mixed fermentation extract according to the present invention there is an advantage that the effect of inhibiting lipoxygenase activity is further improved and can be applied more safely without stimulation.

Hereinafter, the configuration and effects of the present invention will be described in detail with reference to Examples, but the present invention is not limited thereto. The green tea, persimmon leaf, jujube, gojija, gold, silver, chrysanthemum, and azalea used in the area were collected and marketed in Wonju and nearby areas of Gangwon-do, South Korea, and the extraction solvent was purchased from Deoksan, Daejeonghwa Gold, and Samjeon Chemical.

[Example]

< Example  1-5> Extracted Fermented Tea Extract

Green tea, persimmon leaves, jujube, wolfberry, gold and silver, chrysanthemum and rhododendrons were collected, dried and chopped, and 1 kg of these and distilled water were added to the YM medium. Here's the yeast ( Saccharomyces cerevisiae ) was inoculated (concentration of about 10 ⁵ cells / mL) and then incubated for 120 hours while maintaining the conditions of pH 7, temperature 25 ℃. Subsequently, the precipitate and the yeast cells were removed by centrifugation to obtain a fermentation broth.

1 kg of distilled water was added to the fermentation broth, which was extracted by heating at 80 ° C. for 6 hours in an extractor equipped with a cooling condenser, filtered with 300 mesh filter paper, and allowed to stand at room temperature for 1 week to filter the precipitate with Edventech No. 5 filter paper and Whatman GFC 150 mm filter paper. It was filtered twice. After concentrating at 50 ° C. using a vacuum concentrator, the resultant was dried using a spray dryer (manufactured by BUCHI Co., Ltd.) to obtain a petal fermentation extract.

The green tea, persimmon leaf, jujube and goji berries were used in a weight ratio of 1: 1: 1: 1, respectively, and the content was adjusted so as to satisfy the weight ratio of gold and silver, chrysanthemum and azaleas 1: 1: 1. As shown in Table 1.

Tea fermented extract Petal Ferment Extract Dry weight ratio
(Tea: petal extract) Yield (g) green tea Persimmon leaf Jujube Wolfberry Gold coins Chrysanthemum Azalea Example 1 25 g 25 g 25 g 25 g 300 g 300 g 300 g 1: 9 85.7 g Example 2 75 g 75 g 75 g 75 g 235 g 235 g 230 g 3: 7 86.2 g Example 3 125 g 125 g 125 g 125 g 165 g 165 g 170 g 5: 5 89.1 g Example 4 175 g 175 g 175 g 175 g 100 g 100 g 100 g 7: 3 90.4 g Example 5 225 g 225 g 225 g 225 g 33 g 33 g 34 g 9: 1 87.1 g

< Comparative example  1-5> Tea Fermented Extract Extraction

Green tea, persimmon leaves, jujube and wolfberry were harvested, dried and chopped, and 1 kg of these and distilled water were added to the YM medium. Here's the yeast ( Saccharomyces cerevisiae ) was inoculated (concentration of about 10 ⁵ cells / mL) and then incubated for 120 hours while maintaining the conditions of pH 7, temperature 25 ℃. Subsequently, the precipitate and the yeast cells were removed by centrifugation to obtain a fermentation broth.

1 kg of the distilled water was added thereto, and extracted by heating at 80 ° C. for 6 hours in an extractor equipped with a cooling condenser, followed by filtration with a 300 mesh filter paper. Filtered. The resultant was concentrated at 50 ° C. using a vacuum concentrator and dried using a spray dryer (manufactured by BUCHI Co., Ltd.) to obtain a tea fermentation extract.

To Green tea, persimmon leaf, jujube, and wolfberry used in Table 2 are shown.

Sample mixing ratio (dry weight ratio) (green tea: persimmon leaf: jujube: wolfberry) Yield (g) Comparative Example 1 1: 1: 1: 1 (125g: 125g: 125g: 125g) 58.7 g Comparative Example 2 2: 1: 1: 1 (200g: 100g: 100g: 100g) 56.2 g Comparative Example 3 1: 2: 1: 1 (100g: 200g: 100g: 100g) 56.4 g Comparative Example 4 1: 1: 1 2: 1 (100g: 100g: 200g: 100g) 53.4 g Comparative Example 5 1: 1: 1: 1 (100g: 100g: 100g: 200g) 54.9 g

< Comparative example  6-9> Petal Fermented Extract Extraction

Gold, silver, chrysanthemum and rhododendrons were collected, dried and chopped, and 1 kg of these and distilled water were added to the YM medium. Here's the yeast ( Saccharomyces cerevisiae ) was inoculated (concentration of about 10 ⁵ cells / mL) and then incubated for 120 hours while maintaining the conditions of pH 7, temperature 25 ℃. Subsequently, the precipitate and the yeast cells were removed by centrifugation to obtain a fermentation broth.

1 kg of the distilled water was added thereto, and extracted by heating at 80 ° C. for 6 hours in an extractor equipped with a cooling condenser, followed by filtration with a 300 mesh filter paper. Filtered. After concentrating at 50 ° C. using a vacuum concentrator, the resultant was dried using a spray dryer (manufactured by BUCHI Co., Ltd.) to obtain a petal fermentation extract.

Table 3 shows the amounts of gold silver, chrysanthemum and rhododendron used.

Sample Mixing Ratio (Dry Weight Ratio) (Gold Silver: Chrysanthemum: Rhododendron) Yield (g) Comparative Example 6 1: 1: 1 (134g: 133g: 133g) 30.7 g Comparative Example 7 2: 1: 1 (200g: 100g: 100g) 27.2 g Comparative Example 8 1: 2: 1 (100g: 200g: 100g) 26.4 g Comparative Example 9 1: 1: 1 (100g: 100g: 200g) 29.3 g

< Comparative example  10> Green Tea Fermented Extract Extract

It carried out in the same manner as in Example 1, using the green tea alone 1kg as a raw material to obtain a green tea fermentation extract.

< Experimental Example  1> Confirm cell nontoxicity and cell proliferation effect

The cell proliferation effect using cell culture was measured for the fermented extract (dry powder) obtained in Examples and Comparative Examples.

1) Experimental method

Cytotoxicity and proliferation experiments were carried out in the following manner.

Incubate the cells (fibroblast, 3T3) in 96-well microplates at 5,000 cells / well and incubate for 30 minutes in a thermostat and sample by concentration (0.01, 0.05, 0.1, 0.5, 1.0 (%, W, respectively) / V)) and incubated for 72 hours. Thiazoline blue was administered and further cultured for 4 hours. Subsequently, all of the culture solution was discarded and an acidic isopropanol was added to each well of the microplate, followed by stirring for 5 minutes, and then absorbance was measured at 570 nm. As a control group, 10% Fetal Bovine Serum (FBS) medium was used as the sample injection amount to co-culture as an optimal condition for cell growth. .

2) Experiment result

Cell proliferation was calculated by Equation 1 below, and the results are shown in Table 4 below.

Cell growth rate according to the concentration of fermented extract (%) 0.01% 0.05% 0.1% 0.5% 1.0% Example 1 101.1 102.9 107.0 111.7 115.6 Example 2 101.5 103.1 106.3 111.0 116.8 Example 3 100.8 103.6 107.5 112.3 117.9 Example 4 102.5 104.9 109.5 114.6 121.4 Example 5 101.6 103.0 108.2 113.4 117.5 Comparative Example 1 101.8 103.7 106.7 108.9 110.5 Comparative Example 2 101.1 102.5 105.3 107.9 109.1 Comparative Example 3 101.3 102.1 104.5 107.6 109.7 Comparative Example 4 100.1 102.4 104.0 105.3 107.5 Comparative Example 5 100.5 101.8 105.6 107.8 108.3 Comparative Example 6 100.4 102.1 103.4 106.2 107.0 Comparative Example 7 100.7 101.5 103.0 104.9 105.9 Comparative Example 8 100.2 101.6 103.0 105.6 106.3 Comparative Example 9 100.3 101.4 103.7 105.9 106.9 Control group 100 100 100 100 100

As shown in Table 4 above, when the cell proliferation in the optimal conditions for cell growth was 100%, the tea fermentation extract of the present invention was confirmed that the cell is non-toxic and excellent cell proliferation effect.

In particular, green tea, persimmon leaves, jujube and goji berries are 1: 1: 1: 1, gold and silver, chrysanthemum and azalea are used at a ratio of 1: 1: 1, and tea fermented extract and petal fermented extract are at a ratio of 7: 3. The data of Example 4 used can be seen to have the best cell proliferation effect.

< Experimental Example  2> Free radical  Confirmation of scavenging effect (checking antioxidant effect)

Free Radical scavenging effect was measured on the fermented extracts obtained in Examples and Comparative Examples, where quercetin was used as a comparative substance.

1) Experimental method

DPPH (1,1- diphenyl-picryl -2-Hydra quality) method: We performed experiments using (see Blois .MS Nature 181, 1190, 1958 ), DPPH and quercetin used was Sigma (SIGMA) Inc. . 2 ml (concentration: 5, 10, 20, 30 ppm) of fermented extracts of each of Examples and Comparative Examples were added to 1 ml of 0.2 mM DPPH methanol solution, and ethanol solution of quercetin was added as a control, stirred well, and reacted at room temperature for 10 minutes. . Then absorbance was measured at 517 nm, where purified water was used instead of each sample in a blank test.

The free radical scavenging effect was obtained using Equation 2 below and the results are shown in Table 5 below.

2) Experiment result

Free radical scavenging effect according to the concentration of fermented extract (%) 0 ppm 5 ppm 10 ppm 20 ppm 50 ppm SC ₅₀ Example 1 0 19.6 37.7 70.1 85.9 13.8 Example 2 0 19.9 38.4 64.3 77.2 14.5 Example 3 0 16.8 39.7 63.2 70.5 14.4 Example 4 0 22.0 48.6 86.6 92.7 10.4 Example 5 0 20.4 42.3 69.2 80.3 12.9 Comparative Example 1 0 21.5 36.1 72.9 87.7 13.8 Comparative Example 2 0 19.3 37.6 62.6 78.8 15.0 Comparative Example 3 0 17.2 37.4 61.6 69.9 15.2 Comparative Example 4 0 20.7 24.6 70.1 81.5 15.6 Comparative Example 5 0 20.5 34.1 68.3 82.7 14.6 Comparative Example 6 0 20.9 29.5 72.6 90.5 14.8 Comparative Example 7 0 19.1 30.6 62.9 79.9 16.0 Comparative Example 8 0 18.6 33.6 53.9 73.2 18.1 Comparative Example 9 0 20.3 32.9 66.5 83.3 15.1 Control (quercetin) 0 28.4 47.6 90.7 94.3 10.6

As can be seen in Table 5, it can be seen that the tea fermentation extract according to the present invention is superior to the free radical scavenging effect than the respective tea fermentation extract and petal fermentation extract.

In particular, the tea fermentation extract of Example 4 showed the best effect. The free radical 50% scavenging concentration (SC ₅₀ ) of Example 4 is 10.4 ppm, which has a somewhat better effect than 10.6 ppm of quercetin used as a control, and quercetin is a single substance, which is extracted to obtain and use the pure substance. Considering the stability and cost, it can be seen that the free radical scavenging effect of the tea fermentation extract of the present invention is better and more effective in terms of use and production cost.

< Experimental Example  3> Lipoxygenase  Check the inhibitory effect

The inhibitory effect of lipoxygenase activity was measured on the fermented extracts obtained in Examples and Comparative Examples, and quercetin was used as a comparative substance.

1) Experimental method

The experiment was performed using the TBARS method, and linoleic acid, lipoxygenase, thiobarbitulic acid, and quercetin used in the experiment were used by SIGMA. . Fermentation extracts (concentrations: 1, 5, 10, 20, 30 ppm) and quercetin, respectively, were added to 1 ml of linoleic acid at 1 mM, and 0.05 ml of quercetin, respectively, and 0.95 ml of lipoxygenase, followed by stirring well. The reaction was carried out at 25 ° C. for 10 minutes. Then, 0.5 ml of trichloroacetic acid was administered, 1 ml of thiobarbiturlic acid was added, the mixture was heated for 10 minutes, and then cooled in ice water for 2-3 minutes to terminate the reaction. 2 ml of butanol was added to the reaction solution, followed by centrifugation at 4000 g for 5 minutes, and the absorbance was measured at 535 nm. At this time, purified water was used instead of each sample as a control.

Using the following equation (3) to obtain the lipoxygenase inhibitory effect and the results are shown in Table 6 below.

Inhibitory Effect of Lipoxigenase Activity on Concentration of Fermented Extract (%) 0 ppm 1 ppm 5 ppm 10 ppm 20 ppm SC ₅₀ Example 1 0 2.4 11.1 39.1 69.9 13.5 Example 2 0 2.6 11.9 41 71.2 13.0 Example 3 0 3.2 12.9 44.1 68.9 12.4 Example 4 0 4.9 15.1 49.7 80.1 10.1 Example 5 0 2.7 12.2 39.9 70.4 13.3 Comparative Example 1 0 3.1 11.7 33.3 68.1 14.8 Comparative Example 2 0 2.6 10.5 30.8 65.2 15.6 Comparative Example 3 0 2.2 10.2 32.1 64.8 15.5 Comparative Example 4 0 2.9 10.8 29.7 66.5 15.5 Comparative Example 5 0 2.7 10.6 29.9 59.4 16.8 Comparative Example 6 0 3.7 12.5 36.2 80.1 13.1 Comparative Example 7 0 2.5 10.7 31.5 73.2 14.4 Comparative Example 8 0 2.4 11.2 32.8 71.2 14.5 Comparative Example 9 0 2.3 9.9 30.7 69.9 14.9 Control (quercetin) 0 9.8 22.1 56.2 93.3 9.1

As can be seen in Table 6, it can be seen that the tea fermentation extract according to the present invention is superior to the inhibitory effect of lipoxygenase activity than each tea fermentation extract and petal fermentation extract.

In particular, the tea fermentation extract of Example 4 showed the best effect. The lipoxygenase 50% activity inhibitory concentration (IC ₅₀ ) of Example 4 is 10.1 ppm, which is slightly lower than the 9.1 ppm of quercetin used as a control, but quercetin is a single substance, which is extracted to obtain and use the pure substance. Considering the stability and cost, it can be seen that the effect of inhibiting the lipoxygenase activity of the tea fermentation extract is more effective in terms of use and production cost.

< Experimental Example  4> Of hyaluronidase  Confirmation of activity inhibitory effect

The inhibitory effect of hyaluronidase activity on the fermented extracts obtained in Examples and Comparative Examples was measured.

1) Experimental method

By applying the Morgan-Elson method, the final enzyme activity of hyaluronidase was 400NF unit / ml, the final concentration of hyaluronic acid was 0.4 mg / ml, and the active compound Compound 48/80 buffer solution (0.1 mg). / Ml) was used to measure hyaluronidase activity. The sample was dissolved in a buffer solution and green tea extract obtained in Comparative Example 10 was used. For each blank, buffer solution was used instead of enzyme solution.

Using the following equation 4 to determine the effect of inhibiting the hyaluronidase activity and the results are shown in Table 7 below.

Inhibitory Effect of Hyaluronidase Activity on Concentration of Fermented Extract (%) 0 ppm 0.5 ppm 1 ppm 3 ppm 5 ppm IC ₅₀ Example 1 0 19.9 39.5 62.2 79.3 2.7 Example 2 0 18.7 38.9 63.3 78.7 2.7 Example 3 0 18.4 39.1 62.3 78.9 2.8 Example 4 0 24.2 43.4 84.5 95.1 2.2 Example 5 0 19.8 38.7 61.9 78.3 2.7 Comparative Example 1 0 15.9 21.5 46.2 70.3 4.1 Comparative Example 2 0 14.7 20.9 42.8 68.7 4.9 Comparative Example 3 0 14.1 20.4 43.3 67.9 4.8 Comparative Example 4 0 14.0 20.0 42.1 69.0 4.9 Comparative Example 5 0 14.2 20.5 43.9 68.3 4.8 Comparative Example 6 0 19.1 33.4 51.5 73.1 3.0 Comparative Example 7 0 18.0 30.3 49.8 70.5 3.9 Comparative Example 8 0 18.9 30.1 50.3 70.9 3.6 Comparative Example 9 0 18.5 30.0 50.2 71.3 3.7 Comparative Example 10 0 23.1 42.8 67.5 80.9 2.6

As can be seen in Table 7, it can be seen that the tea fermentation extract according to the present invention has a superior inhibitory effect on hyaluronidase activity than the respective tea fermentation extracts and petal fermentation extracts.

In particular, the tea fermentation extract of Example 4 showed the best effect. In the case of fermented tea extract of Example 4, the hyaluronidase 50% activity inhibitory concentration (IC ₅₀ ) was 2.2%, and the hyaluronidase activity inhibitory effect was superior to 2.6% of the green tea extract of Comparative Example 10.

Hereinafter, preferred formulation examples are provided to aid the understanding of the present invention. However, the following formulation examples are merely provided to more easily understand the present invention, and the contents of the present invention are not limited by the formulation examples. At this time, the extract obtained in Example 4 was used as the tea fermentation extract.

< Formulation example  1> flexible lotion (skin lotion)

As shown in Table 8 below, the flexible makeup was prepared according to a conventional method.

Specifically, materials 2, 3, 4, and 8 were added to materials 11 in order, dissolved by stirring, and then materials 5 were heated to about 60 ° C. to be dissolved. Subsequently, 10 substances were added and dissolved, and then 11 substances were added. Then, 1, 6, 7, and 9 substances were added, sufficiently stirred, and aged to prepare a flexible makeup lotion of the title.

number Raw material Content (% by weight) One Tea Tea Fermentation Extract Powder (Example 4) 1.0 2 glycerin 3.0 3 Butylene Glycol 2.0 4 Propylene glycol 2.0 5 Polyoxyethylene Cured Castor Oil 1.0 6 ethanol 10.0 7 Triethanolamine 0.1 8 antiseptic a very small amount 9 Pigment a very small amount 10 Spices a very small amount 11 Purified water Balance

< Formulation example  2> nutrition lotion

As shown in Table 9, the nutrient lotion was prepared according to a conventional method.

Specifically, in Table 9, 10, 11, 13, and 16 were mixed and stirred at 80 to 85 ° C. while mixing and stirring the emulsifier. Subsequently, materials 2, 3, 4, 5, 6, 7, 8, 9, and 12 were dissolved by heating to 80 to 85 ° C and then emulsified. After emulsification, cool down to 50 ℃ while stirring using a stirrer, add material 15, cool to 45 ℃, add material 14, add material 1 at 35 ℃, cool to 25 ℃, and mature. 1 kg of the title nutrition lotion was prepared.

number Raw material Content (% by weight) One Tea Tea Fermentation Extract Powder (Example 4) 1.0 2 Beeswax 1.0 3 Polysorbate 60 1.5 4 Solbitan Sesquioleate 0.5 5 Floating paraffin 10.0 6 Sorbitan stearate 1.0 7 Lipophilic Monostearic Acid Glycerin 0.5 8 Stearic acid 1.5 9 Glyceryl Stearate / Pig-400 Stearate 1.0 10 Propylene glycol 3.0 11 Carboxy Polymer 0.1 12 Triethanolamine 0.2 13 antiseptic a very small amount 14 Pigment a very small amount 15 Spices a very small amount 16 Purified water Balance

< Formulation example  3> Nutrition Cream

Nutritional cream was prepared according to a conventional method as shown in Table 10 below.

Specifically, materials 2, 3, 5, and 8 were heated to 40-45 ° C. with mixing and stirring, and then introduced into the manufacturing unit, followed by an emulsifier, and materials 4, 6 and 7 were added to the manufacturing unit and emulsified. After emulsification was cooled to 35 ℃ while stirring using a stirrer and 10, 11, 12, 13, 21, 14 to the material was added to each of the cooling to 25 ℃ and then aged to prepare 1kg of nutrition cream.

number Raw material Content (% by weight) One Tea Tea Fermentation Extract Powder (Example 4) 1.0 2 Stearic acid 2.0 3 Cetyl alcohol 2.0 4 Glyceryl Monostearate 2.0 5 Polyoxyethylene sorbitan monostearate 0.5 6 Sorbitan sesquioleate 0.5 7 Glyceryl Monostearate / Glyceryl Stearate / Polyoxyethylene Stearate 1.0 8 Wax 1.0 9 Liquid paraffin 4.0 10 Skalan 4.0 11 Caprylic / capric triglyceride 4.0 12 Carboxy Vinyl Polymer 0.3 13 Butylene glycol 5.0 14 glycerin 3.0 15 Triethanolamine 0.5 16 Purified water Balance 17 water 1.0

< Formulation example  4> essence

Essences were prepared according to conventional methods as shown in Table 11 below.

Specifically, nonionic amphiphilic lipids were prepared by homogenizing materials 2, 3, 4, 5 and 6 at a constant temperature. After mixing the nonionic amphiphilic lipids with materials 1, 7, 8, and 14, homogenizing at a constant temperature, passing through a microfluidizer, and then heating material to 50-60 ° C. and gradually adding it to homogenization. The microfluidizer was passed again. Subsequently, 10, 11, 12 and 13 materials were added, dispersed, stabilized, and aged to prepare 1 kg of the title essence.

number Raw material Content (% by weight) One Tea Tea Fermentation Extract Powder (Example 4) 1.0 2 Cytostolol 1.7 3 Polyglyceryl 2-oleate 1.5 4 Ceramide 0.7 5 Steares-4 1.2 6 cholesterol 1.5 7 Dicetyl phosphate 0.4 8 Concentrated glycerin 5.0 9 Macadamia oil 15.0 10 Carboxy Vinyl Polymer 0.2 11 Xanthan Gum 0.2 12 antiseptic a very small amount 13 Spices a very small amount 14 Purified water Balance

Cosmetic composition according to the present invention is applicable to a variety of cosmetics that can be anti-aging or improve the skin.

Claims (14)

Tea fermented extracts obtained from green tea, persimmon leaves, jujube, and wolfberry; And
A cosmetic composition having an antioxidant and anti-inflammatory effect, comprising a fermented tea extract consisting of petal fermented extract obtained from gold silver, chrysanthemum, and rhododendron as an active ingredient. The cosmetic composition of claim 1, wherein the tea fermentation extract has a tea fermentation extract: petal fermentation extract in a weight ratio of 1: 9 to 9: 1. The cosmetic composition of claim 1, wherein the tea fermentation extract is a tea fermentation extract: a petal fermentation extract has a weight ratio of 7: 3 to 9: 1. The method of claim 1, wherein the tea fermentation extract is a cosmetic composition having an antioxidant and anti-inflammatory effect, characterized in that the green tea: gojija: jujube: persimmon leaves in a weight ratio of 1-5: 1-5: 1-5: 1-5 . [Claim 2] The cosmetic composition of claim 1, wherein the petal fermentation extract comprises gold and silver flowers: chrysanthemums and azaleas in a weight ratio of 1 to 5: 1 to 5: 1 to 10. The method of claim 1, wherein the tea fermentation extract and petal fermentation extract
Fermented green tea, wolfberry, jujube, persimmon leaf, gold and silver, chrysanthemum, and azalea with yeast or lactic acid bacteria to obtain fermentation broth,
Cosmetic composition having an antioxidant and anti-inflammatory effect, characterized in that obtained by extracting the fermentation component in the fermentation broth. The method of claim 6, wherein the fermentation broth is inoculated with yeast or lactic acid bacteria (concentration of about 10 ^{5-10 7} cells / ㎖) to green tea, goji berry, jujube, persimmon leaf, gold and chrysanthemum, and azalea, pH 5-8, temperature 20 After culturing for 24 to 120 hours while maintaining the conditions of ~ 45 ℃, the cosmetic composition having an antioxidant and anti-inflammatory effect, characterized in that obtained through the process of removing the yeast cells or lactic acid bacteria. 7. The method of claim 6 wherein the yeast is a Saccharomyces MRS three Levy cyano (Saccharomyces cerevisiae), saccharose in MRS bow radio (Saccharomyces boulardii ), Saccharomyces ellipsoids ), Schzosaccharomyces ellipsoids ) and a cosmetic composition having an antioxidant and anti-inflammatory effect, characterized in that it comprises one selected from the group consisting of these. The method of claim 6, wherein the lactic acid bacteria Lactobacillus ( Lactobacillus) acidophilus ), Lactobacillus casei ), Lactobacillus bulgaricus ), Lactobacillus rhamnosus ), Lactobacillus plantarum ), Lactobacillus fermentum ), Lactobacillus brevis ), Lactobacillus reuteri ), Lactobacillus Confusus ) and a cosmetic composition having an antioxidant and anti-inflammatory effect, characterized in that it comprises one selected from the group consisting of. The cosmetic composition of claim 6, wherein the fermentation component extraction is performed by using the fermentation broth as it is or by adding an extracting solvent after drying the fermentation broth. The method of claim 10, wherein the extracting solvent is water, a lower glycol having 1 to 6 carbon atoms, lower alcohol having 1 to 4 carbon atoms, ethyl acetate, glycerin, and a mixed solvent thereof, using one selected from the group consisting of Cosmetic composition having an antioxidant and an anti-inflammatory effect characterized in. The cosmetic composition according to claim 6, wherein the extraction is obtained through an extraction process of hot water extraction, room temperature extraction, warm extraction, ultrasonic extraction, or supercritical extraction. According to claim 1, wherein the tea fermentation extract is a cosmetic composition having an antioxidant and anti-inflammatory effect, characterized in that it comprises 0.001 to 5% by weight based on the total weight of the cosmetic composition. The method of claim 13, wherein the cosmetic composition is a lotion, nourishing lotion, nourishing cream, massage cream, essence, pack, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, Cosmetic composition having an antioxidant and anti-inflammatory effect, characterized in that it has a formulation of oil, powder foundation, emulsion foundation, wax foundation and spray.