KR102196276B1 - Probiotic composition comprising green tea fermented extract having antibacterial activity against acne-causing resident skin flora and antioxidant efficacy - Google Patents

Abstract

The present invention relates to an antibacterial composition for acne-causing resident skin flora including a green tea fermented extract, and a probiotic composition with improved antioxidant efficacy. The composition is a material derived from natural products and is safe for the skin.

Description

{PROBIOTIC COMPOSITION COMPRISING GREEN TEA FERMENTED EXTRACT HAVING ANTIBACTERIAL ACTIVITY AGAINST ACNE-CAUSING RESIDENT SKIN FLORA AND ANTIOXIDANT EFFICACY}

The present invention relates to an antibacterial composition for acne-causing dermatophytes and a probiotic composition with improved antioxidant efficacy, including a green tea fermented extract.

Human skin is an organ that directly contacts the surrounding environment, and infections such as bacteria and fungi that are involved in various skin diseases easily occur. E.g., blood, including the Staphylococcus aureus in nasal pillow Stability kusu aureus (Staphylococcus aureus) causing maturation in skin

Staphlyococcus epidermidis , an injured germ, and Propionibacterium acnes , an acne germ, are present in the skin.These germs decompose sebum or sweat secreted on the skin. It produces odor, and along with its decomposition products, it irritates the skin and causes inflammation. In particular, Propionibacterium acnes uses a fatty acid degrading enzyme, lipase, to decompose sebum to produce free fatty acids. These free fatty acids not only irritate the skin, but also cause inflammatory lesions such as papules, pustules, and nodules, which are red rashes found in acne.

Antimicrobial compounds such as triclocarban and triclosan have been used as antimicrobial substances against these bacteria, but they are known to cause skin side effects such as skin burning, burning, and redness when used for a long time.

As described above, the conventional antimicrobial synthetic material and cosmetic composition including the same may cause skin irritation, and skin troubles may occur. Accordingly, a new antimicrobial material is required.

Korean Patent Registration No. 10-0883399

It is an object of the present invention to provide a composition for antibacterial against acne-causing dermatophytes comprising a green tea fermented extract.

Another object of the present invention is to provide a cosmetic composition for improving acne comprising the green tea fermented extract.

Another object of the present invention is to provide a probiotic composition containing the green tea fermented extract with improved antioxidant efficacy.

Another object of the present invention is to provide a cosmetic composition comprising the probiotic composition.

Another object of the present invention is to provide a food composition comprising the probiotic composition.

An aspect of the present invention for achieving the above object relates to a composition for antibacterial against acne-causing dermatophytes, including a green tea fermented extract.

In the present invention, “green tea ( Camellia Sinensis )” is an evergreen broad-leaved shrub belonging to the tea tree family, and tea with dried leaves is used in many ways. In particular, it is known to exhibit antioxidant, anti-cancer, cardiovascular, blood lipid-reducing, and blood circulation-stimulating effects.

The "extract" of the present invention refers to an extract obtained by extracting green tea, a dilution or concentrate of the extracts, a dried product obtained by drying the extract, a preparation or purified product of the extract, or a mixture thereof, the extract itself and the extract It includes extracts of all formulations that can be formed using.

The extract may be extracted from natural, hybrid or variant plants of the green tea, and may also be extracted from plant tissue culture.

The method of extracting the extract is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include a hot water extraction method, an ultrasonic extraction method, a filtration method, a reflux extraction method, and the like, which may be performed alone or in combination of two or more methods.

The type of the extraction solvent of the green tea extract is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the extraction solvent include water, C ₁ to C ₄ anhydrous or lower alcohol, a mixed solvent of water and lower alcohol, acetone, 1,3-butylene glycol, ethyl acetate, selected from the group consisting of chloroform It can be used alone or in combination of two or more.

In the present invention, "fermentation" refers to a process of decomposing organic matter using enzymes possessed by the fermentation strain itself. It is known that raw materials that have undergone a fermentation process through a fermentation strain have an effect of at least two to tens of times greater than before fermentation. In particular, metabolites that have undergone fermentation contain various amino acids, organic acids, and antioxidants that are good for the skin, and can promote skin metabolism to smooth the skin texture, and through the fermentation process, particles become smaller and toxicities such as heavy metals are decomposed. It is known that it can relieve skin problems or allergic side effects.

In the present invention, when the green tea extract is fermented, it was confirmed that the antibacterial effect against acne-causing skin flora was excellent. Thus, in the present invention, to provide an antibacterial composition comprising a green tea fermented extract.

Specifically, the fermentation extract may be obtained by inoculating a Lactobacillus Plantarum strain. More specifically, the Lactobacillus Plantarum strain may be Lactobacillus Plantarum AMI-1205 ( Lactobacillus Plantarum AMI-1205) KCCM12713P.

In the present invention, “ Lactobacillus Plantarum AMI-1205” is a novel lactic acid bacteria of Lactobacillus plantarum isolated and identified from Yulmu Kimchi, as of May 8, 2020 by the Korea Microbiology Conservation Center. It was deposited with (accession number KCCM12713P).

In one embodiment of the present invention, green tea was dried, pulverized, mixed with purified water, and heat sterilized at a temperature of 120 to 125°C. The sterilized green tea was inoculated with a Lactobacillus plantarum strain and then fermented at a temperature range of 30 to 37° C. for 3 to 10 days to obtain a green tea fermented extract.

In the present invention, "antibiotic" refers to reducing, preventing, inhibiting or removing the growth or survival rate of microorganisms, and "antibacterial composition" is a composition having an activity to inhibit the growth of microorganisms such as bacteria, and antimicrobial agents It may have the same meaning as antibiotics, which is a generic term, and may include all types of various forms that require antibacterial effect, and may be, for example, cosmetics, food additives, feed additives, pharmaceuticals, or quasi-drugs.

Specifically, the antimicrobial composition may be one having antibacterial activity against Propionibacterium acnes .

In one embodiment of the present invention, as a result of confirming the antimicrobial activity by concentration using Lactobacillus plantarum bacteria, the minimum inhibitory concentration against acne-causing skin flora is low, and overall growth inhibitory activity against acne-causing skin flora It was confirmed that the effect was excellent (Tables 2 and 3).

Another aspect of the present invention may relate to a cosmetic composition for improving acne comprising the composition.

In the present invention, “acne” refers to acne vulgaris, a skin disease caused by increased production of sebum in the pores and abnormal keratinization of skin epithelial cells to block the opening of the pores, where acne-causing bacteria proliferate and cause inflammation. Is one of them. The “acne improvement” refers to improving the skin condition by inhibiting and inhibiting the growth of bacteria that cause acne, thereby inhibiting pore closure and excessive secretion of sebum.

In one embodiment of the present invention, it was confirmed that the fermented green tea extract of the present invention fermented with Lactobacillus plantarum has excellent antibacterial activity against Propionibacterium acnes , one of the acne-causing bacteria (Table 2). And Table 3). Through this, it was confirmed that it can be effectively used in a cosmetic composition for improving acne.

Another aspect of the present invention relates to a probiotic composition containing a green tea fermented extract with enhanced antioxidant efficacy.

In the present invention, “antioxidation” refers to an action of inhibiting oxidation, and in the human body, an oxidation promoting substance and an oxidation inhibitor are in balance, but due to various factors, the balance is lost and the direction of promoting oxidation When inclined toward, oxidative stress is induced in a living body, leading to cell damage and pathological diseases. Reactive oxygen species (ROS), which are the direct cause of oxidative stress, are chemically unstable and highly reactive, so they can easily react with various biomaterials such as DNA, proteins, lipids, and carbohydrates. Attacks cause irreversible damage to cells and tissues, or cause mutations, cytotoxicity and cancer, and are also a direct cause of aging. By removing or reducing these reactive oxygen species, antioxidant effects are obtained, preventing aging and maintaining health.

In the present invention, "probiotics" is a concept collectively referred to as living microorganisms that exert beneficial effects on health and are also called lactic acid bacteria. In general, it is a generic term for bacteria in the intestines that help health, and recently, it has been used in various ways for beauty purposes such as cosmetics or health functional foods.

Specifically, the fermentation extract may be obtained by inoculating a Lactobacillus Plantarum strain, and more specifically, the Lactobacillus Plantarum strain is Lactobacillus Plantarum AMI-1205 ( Lactobacillus Plantarum AMI-1205) It may be KCCM12713P.

In one embodiment of the present invention, the antioxidant effect was confirmed by measuring the free radical scavenging activity. In particular, the green tea fermented extract obtained using the Lactobacillus plantarum strain was compared to the green tea fermented extract fermented with other strains. It was confirmed that the antioxidant effect was significantly increased (Tables 4 and 5), and it was confirmed that the antibacterial activity enhancing effect of the probiotic composition is excellent.

Another aspect of the present invention relates to a cosmetic composition comprising the probiotic composition.

Specifically, the cosmetic composition may be for antioxidant.

The green tea fermented extract may be included in a dry weight of 0.001% to 30.0% by weight based on the total weight of the cosmetic composition. More specifically, it may be included in 0.01% to 20% by weight. There is an advantage in that excellent acne improvement and antioxidant effects appear within the above range, and there is an advantage in that the formulation of the composition is stabilized.

The cosmetic composition of the present invention is a solution, external ointment, cream, foam, nutrient lotion, flexible lotion, pack, softening water, emulsion, makeup base, essence, soap, liquid detergent, bathing agent, sunscreen cream, sun oil, suspension, emulsion It can be prepared in a formulation selected from the group consisting of liquid, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch, and spray, but is limited thereto. no.

The cosmetic composition of the present invention may additionally include one or more cosmetically acceptable carriers blended in general skin cosmetics, and as common ingredients, for example, oil, water, surfactants, moisturizers, lower alcohols, thickeners , A chelating agent, a colorant, a preservative, a fragrance, and the like may be appropriately blended, but are not limited thereto.

The cosmetically acceptable carrier included in the cosmetic composition of the present invention varies depending on the formulation of the cosmetic composition.

When the formulation of the present invention is an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc. May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, etc. may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohard It may include a propellant such as locarbon, propane/butane or dimethyl ether, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil, and the like may be used, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, micro Crystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used, but are not limited thereto. These may be used alone or in combination of two or more.

In addition, the composition of the present invention may be used as a transdermal administration method such as direct application or spraying on the skin, and the administration route of the composition of the present invention may be administered through any general route as long as it can reach the target tissue.

The amount of use of the composition of the present invention may be appropriately adjusted according to individual differences or formulations such as age and degree of lesions, and may be used from one week to several months by applying one or several times a day to the skin.

Another aspect of the present invention relates to a food composition comprising the probiotic composition.

The description of the “probiotics” is the same as described above.

Specifically, the food composition may be for animals, and more specifically may be for companion animals.

In the present invention, "Companion animal" refers to animals that live with humans, such as dogs, cats, hamsters, rabbits, chickens, iguanas, guinea pigs, hedgehogs, gerbils, snails, raccoons, parrots, lizards, and fish including tropical fish. Includes all regardless. The health functional food for companion animals refers to all foods consumed by companion animals to promote and/or maintain the health of companion animals or maintain physical condition.

Foods to which the green tea ferment extract of the present invention can be added include meat, bread, sausage, chocolate, snacks, candy, confectionery, ramen, pizza, other noodles, gums, dairy products including ice cream, various soups, beverages, tea , Vitamin complexes, and health functional supplements.

In an embodiment of the present invention, a feed having excellent antioxidant and probiotic activity was prepared by spraying and drying the green tea fermented extract at the last step of the conventional animal feed manufacturing process.

The type of food may specifically be a health functional food. The health functional foods include various nutrients, vitamins, minerals (electrolytes), synthetic flavors and flavoring agents such as natural flavors, colorants and enhancers (cheese, chocolate, etc.), pectic acid and its salts, organic acids, protective colloidal growth It may contain an agent, a pH adjuster, a stabilizer, a preservative, glycerin, alcohol, a carbonation agent used in carbonated beverages, and the like. These components may be used alone or in combination, and the proportion of these additives is generally selected from 0.001 to 50 parts by weight per total weight of the composition.

The health functional food is a food that emphasizes the biological regulation function of food, and is a food that has added value to act and express it for a specific purpose by using physical, biochemical, and bioengineering methods. Ingredients of these health functional foods are designed and processed to fully exert the body's control functions related to body defense, regulation of body rhythm, prevention and recovery of diseases to the living body, and food additives, sweeteners, or functional foods acceptable as foods. It may contain raw materials.

The health functional food may be any one formulation selected from the group consisting of tablets, granules, powders, capsules, liquid solutions, and pills, but is not limited thereto.

In addition, the type of food may be a food additive, and the food additive refers to a substance used by a method such as adding, mixing, infiltrating or adding to food for manufacturing, processing, or preserving food. The food additives include natural products and synthetic products, and can be classified according to functions and uses. Currently, 370 kinds of chemical synthetic products and 50 kinds of natural additives are approved as food additives in Korea. Depending on the purpose, preservatives, fungicides, antioxidants, coloring agents, coloring agents, bleaching agents, seasonings, sweeteners, flavoring agents, expanding agents, strengthening agents, and improving agents , Emulsifiers, thickeners and stabilizers, coating agents, gum base agents, defoaming agents, solvents, releasing agents, insect repellents, quality improvers and other additives for food manufacturing.

The form of the food additive may include a powder, granule, tablet, capsule or liquid form, and specifically may be a capsule form, but is not limited to the form.

When the green tea fermented extract of the present invention is used as a food composition, the green tea fermented extract may be added as it is or used with other foods or food ingredients, and may be appropriately used according to a conventional method. The mixing amount of the green tea fermented extract may be appropriately determined according to the purpose of use (prevention, health or improvement, therapeutic treatment). Furthermore, as an antibacterial use, it can be applied without limitation to various household products, companion products, and the like.

The green tea fermented extract of the present invention is excellent in antibacterial and antioxidant effects against acne-causing skin flora.

In addition, the composition of the present invention is a natural substance-derived material that is safe for the skin and has high utilization as a cosmetic composition for improving acne and/or for antioxidant purposes.

The effects of the present invention are not limited to the above effects, and should be understood to include all effects that can be deduced from the configuration of the invention described in the detailed description or claims of the present invention.

Hereinafter, the present invention will be described in detail by examples. However, the following examples are only illustrative of the present invention, and the present invention is not limited by the following examples.

Example 1-2. Preparation of green tea fermented extract

The green tea outpost washed with purified water and dried was coarsely pulverized and extracted with hot water at 121°C for 15 minutes with 20 times the extraction solvent (purified water). Inoculum for the fermentation was used for the Lactobacillus Planta room (Lactobacillus Plantarum) cultured in a sterilized lactic acid bacteria culture medium (Lactobacillus MRS Broth, deep nose, USA), to recover only the cells after the culture is completed, 1x10 ⁹ cfu / ml concentration Was inoculated into green tea extract. At this time, the fermentation strain was Lactobacillus plantarum AMI-1205 ( Lactobacillus plantarum AMI-1205) KCCM12713P, which was directly isolated from Yulmu kimchi, and Lactobacillus Plantarum , which was sold in Korea Microbial Conservation Center. KCCM 11322 was used. Fermentation was carried out while maintaining at 25 to 35 °C for 1 to 3 days using a fermentation tank. The fermented green tea after the fermentation was completed was centrifuged to remove the cells, and finally filtered using 0.2 μm filter paper. The extracted filtered green tea extract was lyophilized and used.

Comparative Example 1. Preparation of green tea extract

The green tea outpost washed with purified water and dried was coarsely pulverized and extracted with hot water at 121°C for 15 minutes with 20 times the extraction solvent (purified water). The extracted green tea extract was centrifuged to recover the extraction supernatant, and the extract was filtered through a filter having a size of 0.2 µm to prepare a green tea extract. The extracted filtered green tea extract was lyophilized and used.

Comparative Example 2-7. Manufacture of fermented green tea extract

The method for preparing the fermented green tea extract was the same as in Example 1-2, except that the fermented strain was purchased from the Korea Microbial Conservation Center instead of Lactobacillus plantarum, Leuconostoc mesenteroides , and Lactobacillus curbatus ( Lactobacillus curvatus ) and Leuconostoc citreum were used, respectively.

Examples and comparative examples are shown in Table 1 below.

Example ingredient Strain Example 1 green tea Lactobacillus Plantarum AMI-1205 KCCM12713P Example 2 Lactobacillus plantarum KCCM 11322 Comparative Example 1 - Comparative Example 2 Leuconosstoc citreum AMI-1227 Comparative Example 3 Leuconostoc mesenteroides AMI-1228 Comparative Example 4 Lactobacillus curvatus AMI-1214 Comparative Example 5 Leuconostoc mesenteroides AMI-1208 Comparative Example 6 Leuconostoc mesenteroides AMI-1209 Comparative Example 7 Leuconostoc mesenteroides AMI-1224

Experimental Example 1. Confirmation of antibacterial effect on fermentation strains

In order to confirm the antibacterial activity against the fermented strain used in the green tea fermented extract of the present invention, the minimum inhibitory concentration (MIC) was measured by the broth micro dilution method.

First, after putting Propionibacterium acnes of OD600 = 0.1 in a 96 well plate, the fermentation strain Lactobacillus plantarum used in Examples 1 and 2 and Comparative Examples 1 to 7 ( Lactobacillus plantarum ), Lactobacillus mesenteroides ( Leuconostoc mesenteroides ), Lactobacillus curvatus ( Lactobacillus curvatus ), and Leuconostoc citreum ( Leuconostoc citreum ) were used, respectively.

The strains were treated by diluting twice from a maximum of 50 mg/mL. Each plate was incubated at 37º for 24 hours, and then the MIC (Minimum Inhibitory concentration) value was calculated as the minimum concentration for inhibiting the growth of cells. Propionibacterium acnes ( Propionibacterium KCTC 3314) was used as a test bacterium. All experiments were repeated at least 3 times.

All experiments were repeated at least 3 times.

compound MIC value (mg/mL) Propionibacterium Example 1 25 Comparative Example 2 50 Comparative Example 3 50 Comparative Example 4 25 Comparative Example 5 50

As shown in Table 2, it was confirmed that the Lactobacillus plantarum strain showed superior antibacterial activity compared to other strains.

Experimental Example 2. Confirmation of antimicrobial efficacy against Lactobacillus plantarum fermented strains and test strain cultures

An experiment was conducted to compare the antimicrobial efficacy of the culture broth of Lactobacillus plantarum AMI-1205 KCCM12713P of Example 1, which is a fermentation strain of the green tea fermentation extract of the present invention, and Lactobacillus plantarum KCCM 11322, of Example 2, which is a known strain.

In order to measure the minimum inhibitory concentration (MIC) of the strain against acne-causing dermatophytes, analysis was performed by the broth micro dilution method.

First, after putting Propionibacterium acnes of OD600 = 0.1 in a 96 well plate, Examples 1 and 2 and Comparative Examples 1 to 7 were diluted twice from a maximum of 50 mg/mL and treated. Each plate was incubated at 37º for 24 hours, and then the MIC (Minimum Inhibitory concentration) value was calculated as the minimum concentration for inhibiting the growth of cells. Propionibacterium acnes ( Propionibacterium KCTC 3314) was used as a test bacterium. All experiments were repeated at least 3 times.

All experiments were repeated at least 3 times.

compound MIC value (mg/mL) Propionibacterium Example 1 0.78 Example 2 3.13

As shown in Table 3, when the green tea extract was fermented with Lactobacillus plantarum, it was confirmed that the antimicrobial activity against acne-causing skin flora was excellent by lowering the minimum inhibitory concentration.

Experimental Example 3. Antioxidant Effect of Green Tea Fermented Extract Confirmation

3-1. ABTS ⁺ Confirmation of radical scavenging activity

ABTS ⁺ [2,2'-Azino-Beads (3-ethyl benzothiazoline-6-sulfonic acid) (ABTS ⁺ [2,2'-Azino-bis (3-ethyl benzothiazoline-6-sulfonic acid)]) radical scavenging activity Is capable of measuring both hydrogen-donating antioxidants and chainbreaking antioxidants, and is a method applicable to both the aqueous and organic phases.

The 7 mM ABTS solution was mixed so that potassium persulfate became 2.45 mM, reacted in the dark for about 24 hours, and the ABTS ⁺ solution was mixed with the sample and reacted for 6 minutes in the dark, and the absorbance was measured at 734 nm. The resulting value was expressed as a percentage (%) of the radical scavenging activity through calculation using the following equation 1 by comparing the extract sample-treated group and the untreated group.

[Equation 1]

ABTS ⁺ scavenging activity (%) = [1-(absorbance of sample treatment group in Example or Comparative Example / absorbance of sample untreated group)] X 100

ABTS ⁺ Elimination (%) Concentration (μg/ml) 6.25 12.5 25 50 100 Example 1 Average 40.67 63.90 89.03 99.74 99.40 Standard Deviation 1.13 1.76 0.15 0.00 0.83 Example 2 Average 37.3 50.8 77.8 99.7 99.8 Standard Deviation 0.4 0.7 0.6 0.1 0.1 Comparative Example 1 Average 34.3 56.7 62.2 98.8 100.2 Standard Deviation 0.8 1.2 1.1 0.1 0.0 Comparative Example 2 Average 25.7 40.9 65.8 93.8 99.7 Standard Deviation 1.1 0.9 0.6 0.6 0.1 Comparative Example 3 Average 26.8 43.2 68.9 96.0 99.8 Standard Deviation 0.5 0.4 0.1 0.2 0.0 Comparative Example 4 Average 37.6 51.1 77.9 100.2 100.2 Standard Deviation 0.2 0.9 0.5 0.1 0.2 Comparative Example 5 Average 27.4 44.8 72.2 97.4 100.1 Standard Deviation 0.5 1.9 0.6 0.2 0.1 Comparative Example 6 Average 28.8 46.1 74.0 98.3 99.6 Standard Deviation 0.8 0.8 0.7 0.1 0.4 Comparative Example 7 Average 28.3 45.3 70.9 96.6 99.9 Standard Deviation 0.4 0.4 0.5 0.5 0.1

As shown in Table 4, it was confirmed that the ABTS ⁺ scavenging ability of the green tea fermented extract using Lactobacillus plantarum was superior to that of the fermented extract using other strains.

3-2. DPPH scavenging activity confirmation

After adding 20 μL of each of the extracts of Examples 1 and 2 and Comparative Examples 1 to 7 to 180 μL of the DPPH solution, blocking the light from the outside, reacting at room temperature for 30 minutes, and then measuring the absorbance at 517 nm to decrease the absorbance for the control group. The antioxidant activity was shown as a ratio. At this time, as a control for determining the rate of decrease in absorbance, ascorbic acid, an antioxidant, was used, and the same amount as the sample was added and measured by the same method. Then, DPPH scavenging activity was calculated according to Equation 2 below.

[Equation 2]

DPPH scavenging activity (%) = [1-(absorbance of sample treatment group in Example or Comparative Example / absorbance of sample untreated group)] X 100

DPPH elimination (%) Concentration (μg/ml) 6.25 12.5 25 50 100 Example 1 Average 23.3 36.4 56.3 76.0 90.8 Standard Deviation 2.2 2.6 1.6 5.3 1.8 Example 2 Average 21.7 34.7 52.5 74.4 87.1 Standard Deviation 1.7 1.7 1.2 0.8 0.5 Comparative Example 1 Average 18.6 28.1 44.9 67.0 86.4 Standard Deviation 0.8 0.4 0.0 0.4 1.4 Comparative Example 2 Average 16.1 23.9 37.9 58.0 74.8 Standard Deviation 0.4 0.5 0.1 2.2 4.3 Comparative Example 3 Average 17.0 25.7 40.6 59.7 80.5 Standard Deviation 0.7 0.3 0.5 1.6 0.1 Comparative Example 4 Average 19.9 31.8 51.9 73.8 85.0 Standard Deviation 0.5 1.0 0.3 1.4 0.3 Comparative Example 5 Average 18.3 28.5 41.5 55.4 75.1 Standard Deviation 0.7 1.3 2.4 3.1 4.7 Comparative Example 6 Average 17.7 29.4 43.2 61.4 73.6 Standard Deviation 2.9 0.0 0.3 3.1 4.7 Comparative Example 7 Average 17.5 25.8 42.1 64.1 82.6 Standard Deviation 0.5 1.4 1.0 1.2 1.8

As shown in Table 5, when the green tea extract is fermented with Lactobacillus plantarum, DPPH scavenging ability compared to the non-fermented green tea extract (Comparative Example 1) or the extract fermented with other strains (Comparative Examples 2 to 7) This excellence was confirmed (Fig. 2).

Through the experiment to confirm the ABTS ⁺ and DPPH scavenging ability, it was confirmed that the antioxidant effect of the green tea ferment extract using the Lactobacillus plantarum strain was excellent, and it was confirmed that the antioxidant effect was improved in a concentration-dependent manner. It suggests that the green tea fermented extract of the present invention can be used as a cosmetic composition for antioxidant purposes.

Preparation Example 1. Essence Preparation

A cosmetic composition of an essence formulation was prepared as shown in Table 6 below (unit: wt%).

ingredient Content (% by weight) Green tea ferment extract of Example 1 or 2 5 Cetearyl alcohol 2 Cetearyl glucoside One Jojoba seed oil 5 Silica 0.5 glycerin 10 Purified water To 100

Preparation Example 2. Cream Preparation

As shown in Table 7 below, a cream-formed cosmetic composition was prepared (unit: wt%).

ingredient Content (% by weight) Green tea ferment extract of Example 1 or 2 5 Stearic acid 15 ethanol One Potassium hydroxide 0.7 glycerin 5 Propylene glycol 3 antiseptic Appropriate amount incense Appropriate amount Purified water to 100

Manufacturing Example 3. Preparation of feed for animal health functional food

In the process after oil coating, which is the last process of manufacturing conventional feed (AT Bio), the green tea ferment extract prepared in Example 1-2 was sprayed with 1% by weight and dried, finally, the green tea ferment extract A feed containing excellent antioxidant and probiotic activity was prepared.

The above description of the present invention is for illustrative purposes only, and those of ordinary skill in the technical field to which the present invention pertains will be able to understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative and non-limiting in all respects. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as being distributed may also be implemented in a combined form.

The scope of the present invention is indicated by the claims to be described later, and all changes or modified forms derived from the meaning and scope of the claims and the concept of equivalents thereof should be construed as being included in the scope of the present invention.

Korea Microorganism Conservation Center (overseas) KCCM12713P 20200508

Claims (12)

As a composition for antibacterial against acne-causing dermatophytes comprising a fermented green tea extract,
The fermentation extract is obtained by inoculating the strain Lactobacillus Plantarum AMI-1205 ( Lactobacillus Plantarum AMI-1205) KCCM12713P, antibacterial composition. delete delete The method of claim 1,
The antibacterial composition is that of propionibacterium acnes ( Propionibacterium acnes ) to have an antibacterial activity, antibacterial composition. A cosmetic composition for improving acne, comprising the composition of claim 1 or 4. As a probiotic composition with enhanced antioxidant efficacy comprising a green tea fermented extract,
The fermentation extract is obtained by inoculating the strain Lactobacillus Plantarum AMI-1205 ( Lactobacillus Plantarum AMI-1205) KCCM12713P, a probiotic composition. delete delete A cosmetic composition comprising the probiotic composition of claim 6. The method of claim 9,
The cosmetic composition is for antioxidants, cosmetic composition. A food composition comprising the probiotic composition of claim 6. The method of claim 11,
The food composition is for animals, food composition.