KR101484886B1 - Anti-wrinkle cosmetic composition - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving wrinkles, and more particularly to a cosmetic composition for improving wrinkles containing a fermented extract of black wormwood as an active ingredient. The cosmetic composition for wrinkle improvement according to the present invention is excellent in skin-safe and collagen synthesis promoting effect, and is excellent in skin wrinkle improving effect, wound healing effect and persistence of effect.

Description

[0001] Anti-wrinkle cosmetic composition [

The present invention relates to a cosmetic composition for improving wrinkles, and more particularly, to a cosmetic composition for improving wrinkles, which is excellent in skin promoting effect and promotes collagen synthesis, and has excellent skin wrinkle improving effect, wound healing effect and sustained effect .

Collagen, a major component of the extracellular matrix, is a major substrate protein in the fibroblasts of the skin and is present in extracellular epilepsy. It is also an important protein that accounts for about 30% of the total weight of bioproteins, and has a solid triple helix structure. Collagen forms most of the organic matter in the skin, tendons, bones and teeth, especially in bone and skin (jaw). In most other structures, it exists as a fibrous inclusion.

Collagen is a relatively weak immunogen, partly due to the blocking of the latent antigenic determinant by the helical structure of the collagen, which also causes the collagen to be resistant to proteolysis. The main functions of collagen are known to be mechanical rigidity of the skin, joint strength of the connective tissues and binding force of tissues, support of cell adhesion, induction of cell division and differentiation (when organism grows or heals the wound) (Van der Rest et al. , Ann NY Acad Sci, 1990).

Such collagen is reduced by aging and photoaging by ultraviolet irradiation, which is known to be closely related to the wrinkling of the skin (Arthur K. Balin et al., Aging and the skin, 1989). Collagen also plays an important role in wound healing and can accelerate the synthesis of collagen in the damaged epithelium, allowing quick, scarless wound healing.

Conventionally, products using collagen in a composition for external application for skin such as cosmetics or ointment have been marketed in order to utilize the skin moisturizing effect and wound healing effect of collagen. However, these products apply collagen itself to the skin surface, It is impossible to expect a moisturizing action or a wound healing effect, so that it can not be said that it exhibits essential skin function improvement and wound healing function.

In order to solve this problem, there has been a growing interest in collagen synthesis promoting substances. Vitamin C, retinoic acid, transforming growth factor (TGF) (Cardinale G. et al. (Japanese Patent Application Laid-open No. Hei 8-231370), betulinic acid (Japanese Patent Application Laid-Open No. 8-208424) Chlorella extract (JP-A-9-40526, JP-A-10-36283, fibroblast proliferation promoting action), and the like.

However, these materials have limited use due to safety problems such as irritation and reddening when applied to the skin, or the effect is insignificant and virtually no effect of skin function improvement or wound healing can be expected. Therefore, it is urgently required to develop a novel composition for external skin application which is safer and more effective in living body than existing skin external composition compositions.

On the other hand, fermentation is a process of decomposing organic matter using enzymes possessed by microorganisms. Fermentation and corruption proceed by a similar process, but decomposition is called fermentation when substances that are useful for our lives are made, and when they make odorous or harmful substances, it is called corruption. Therefore, if the herb medicine is fermented using microorganisms, the toxicity of the herb medicine is reduced, the low molecular weight is easily digested, and the transdermal absorption upon application to the skin becomes easy, and the bioavailability is improved.

The herbal medicines themselves can exhibit pharmacological effects, but the pharmacological effects can be further strengthened by fermentation or harmful components can be produced by the metabolism of microorganisms, so that a new pharmacological effect can be exhibited which is not present in the herbal medicine before fermentation.

In order to solve the problems of the prior art as described above, it is an object of the present invention to provide a cosmetic composition for improving wrinkles, which is excellent in skin-promoting effect and collagen synthesis promoting effect and is excellent in skin wrinkle improving effect, wound healing effect, .

Another object of the present invention is to provide a cosmetic composition for improving wrinkles in which the pharmacological effect of each medicinal substance is further enhanced through fermentation, thereby further enhancing collagen synthesis promoting effect.

In order to attain the above object, the present invention provides a method for producing a mushroom of the present invention, comprising the steps of: preparing a mushroom of the order Hwanggi, Myeongseo, a bank, a mulberry, a skunk mushroom, a yellowish white, Characterized in that it comprises as an active ingredient a fermented extract of a medicinal substance selected from the group consisting of rosemary extract, rosemary extract, rosemary extract, bamboo shoot, blooming lotus root, castor rosemary, The present invention provides a cosmetic composition for improving wrinkles.

The fermentation extract can be obtained by adding a medicinal substance to yeast, lactic acid bacteria or bifidobacteria and culturing the same.

The fermented extract can be obtained by inoculating fungi into a medicinal material and solid fermentation.

The medicinal fermentation extract is preferably contained in the cosmetic composition in an amount of 0.0001 to 10% by weight.

The cosmetic composition may be formulated into ointment for external use, softening longevity, nutritional lotion, nutritional cream, massage cream, essence, pack, emulsion, oil gel and the like.

The cosmetic composition for wrinkle improvement according to the present invention enhances the pharmacological effect of each medicinal substance through fermentation while safely protecting the skin, thereby effectively promoting collagen synthesis, and thus has excellent skin wrinkle improving effect, wound healing effect and persistence of effect thereof.

Hereinafter, the cosmetic composition for wrinkle improvement of the present invention will be described in detail.

The medicinal plant extracts described in the present invention can be used as a medicinal herb extract or a medicinal herb extract of the present invention as a medicinal herb extract or a medicinal herb extract, Refers to an extract obtained by extracting a medicinal substance selected from at least one kind selected from the group consisting of jade, jade, blooming, turmeric, castor, bamboo, bamboo, hodo, zucchini, hoiocer, safflower, Also, the medicinal fermentation extract means a secondary extract obtained by inoculating and culturing the above-mentioned medicinal plant extract into yeast, lactic acid bacteria or bifidus microorganisms, or obtained by solid fermentation by adding fungi to commercially available medicinal products or pulverized products.

The present inventors have conducted studies to develop new collagen synthesis promoting materials for copper and plants fermented by useful microorganisms in order to develop a substance having promoting effect on collagen synthesis at a cellular level using human-derived fibroblasts. As a result, , Aloe vera, rosemary, green beans, oyster mushroom, jade, mulberry, mulberry, mud wormwood, jade tree, jade, mushroom, bloom, Fermented liquids such as Phellinus linteus, Pseudomonas aeruginosa, Pseudomonas aeruginosa, Pumpkin, Hoioche, Safflower, Hwangjeong, Persimmon leaves have remarkably excellent collagen synthesis promoting effect compared to the respective extracts.

The cosmetic composition for wrinkle improvement according to the present invention is a cosmetic composition for improving wrinkles, which comprises at least one member selected from the group consisting of Hwanggi, Hwangseongcho, Banks, Wuin, Skimmer mushroom, Yellowish white, Nonfoliated, Aloe, Quercus, Birch, Rose, Kidney bean, As an active ingredient, a fermented extract of a medicinal substance selected from the group consisting of jade, jade, jade, blooming, carnivorous, castor, bamboo, hoof, hodo, zucchini, hoiocer, safflower,

Astragalus membranaceus is a perennial herb that belongs to Leguminosae, and is used for diuretics, exanthema, and tonsils. The Houttuynia cordata is a perennial herb belonging to Saururaceae and is used for jaundice and hepatitis. Ginkgo biloba (Ginkgo biloba) is a perennial herbaceous tree belonging to the banking family (Gingkoaceae) and is used for heart disease, hypertension, asthma and seawater. Coix lachryma-jobi var.ma-yuen is a first-generation herb of Gramineae and is used for chronic arthritis and abortion. The above-mentioned Schizophyllum commune belongs to the skirt mushroom family. The Phellodendron chinense is used for the treatment of diseases caused by mushrooms, which are separated from the cork layer of the cedar wood belonging to the Rutaceae family. Eriobotrya japonica is a dried leaf of the loquat tree belonging to Roosaceae, and is used for chronic bronchitis, seawater and vomiting. Aloe ferox is a perennial herb that belongs to Riliaceae and is used for antibacterial, anti-ulcer, and anti-cancer. Castanea crenata is a chestnut astringent which belongs to the Fagaceae family. The birch (Betula platyphylla) belongs to Betulaceae and is used for jaundice, diarrhea, nephritis, pulmonary tuberculosis and gastritis. The rose (Rosa hybrida) is a shrubs of Rosaceae and is used for ornamental or fragrant. The above-mentioned kidney bean (Phaseolus vulgaris) is an annual herb that belongs to leguminosae, and is used for medicines such as boils, diuresis, diarrhea, each edema and edema. The Pleurotus ostreatus belongs to the Pleurotus ostreatus, and is used for edible purposes in the aged trees of hardwoods. Camompsis grandiflora is a vine plant belonging to Bignoniaceae and is used for the treatment of diseases caused by eosinophilia or hematopoiesis. The Opuntia ficus is also referred to as an open-handed cactus, and is used for skin diseases, rheumatism, and burns. The bananas (Areaca catechu) refers to the fruit of the bananas belonging to the Palmae (Palmae), and is used for anthelmintics, authoritics and the like. The Artemisia capillaries belong to the family Asteraceae (Compositae) and are used for diuretics and jaundice. The Mallotus japonicus is a deciduous arboreous tree belonging to the main pole and is used for gastritis, gastric ulcer, and stomach cancer. Polygonatum officinale is a perennial plant belonging to the Liliaceae family. It uses roots of divergent for diabetes, myocardial degeneration, hyperlipidemia and the like. Incaria sinensis belongs to Rubiaceae, and is used for seizures, paralysis, and hypotension. Tribulus terrestirs is a fruit of the southern stallion, a perennial herb belonging to Zypophyllaceae, and is often used in ophthalmic diseases. Hamamellis japonica is a deciduous deciduous witch hazel, which has skin sedative and protective action. The castor (Ricinus communis) is a mature seed of the mackerel, a perennial herb belonging to the Euphorbiaceae, and is used for subjugation, constipation, scab, burns. Draconis resina is dried in the fruits and stems of the giraffins, such as perennial evergreen belonging to the Palmae family, and is used for bleeding, abrasion and physiological impurity. Cucumis sativus is a fruit of cucumber, an annual herb belonging to the genus Cucurbutaceae. Juglans regia is a seed of walnut belonging to the genus Juglandaceae. It is used for paddy rice, uterine bleeding, gastric hyperplasia, lower abdominal pain, acute chronic gonorrhea and the like. The amber (Cucurbita moschata) is an annual herb that belongs to the genus Cucurutaceae and is used for edible and asthma treatment. The Saxifraga stolonifera is a perennial herb belonging to the Saxifragaceae and is used for pertussis, burns, and statues. The safflower (Carthamus tinctorius) is a biennial herb that belongs to the Compositae family and is used for women's diseases, abdominal pain, Polygonatum sibiricum is a perennial herb that belongs to Liliaceae and is dried in the roots of the layered roundworm, and is used for diabetes. The persimmon leaves (Diospyroskaki) are used for medicinal purposes by drying the leaves of persimmon belonging to Ebenaceae, and are used for the paralysis and the high blood circulation.

The medicinal materials may be prepared by a conventional method, for example, by extracting with an organic solvent such as distilled water, anhydrous or low-boiling alcohol having 1 to 4 carbon atoms, concentrating under reduced pressure and then drying to obtain a first extract . Specifically, there are a lot of plants such as hwanggi, hwangseongcho, bank, waiin, skirt mushroom, yellowish white, nonfibre, aloe, chestnut, birch, rose, kidney bean, oyster mushroom, , 5 to 20 parts by volume of water based on the dry weight of the pulverized product, 1 to 4 parts by weight of anhydrous or water-containing anhydride having a carbon number of 1 to 4, In an extractor with a reflux condenser with an organic solvent such as a lower alcohol, the extracts of the medicines are extracted by heating at 50 to 100 ° C for 1 to 5 hours. After filtration with a filter cloth, the residue is further extracted one or more times by the same method. The extracts are combined, concentrated under reduced pressure, and lyophilized or spray dried to obtain a dry extract.

The herbal extracts obtained as described above may be added to yeast, lactic acid bacteria or bifidus bacteria and cultured to obtain a final fermented extract. At this time, it is preferable that the medicinal plant extract is added to the basic medium of yeast, lactic acid bacteria or bifidobacteria in an amount of 1 to 20% by weight. In addition, the pharmaceutical fermentation extract may be obtained by inoculating the commercially available medicinal product itself or a slice or powder into a fungi, followed by solid fermentation.

The yeast may be Saccharomyces cerevisiae , Schizosaccharomyces ellipsoids , Saccharomyces boulardii or the like.

The lactic acid bacteria may be selected from the group consisting of Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus confusus, Lactobacillus fermentum and Lactobacillus brevis .

Examples of the bifidobacteria include Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum , Bifidobacterium infantis , and the like.

The fungus can be a medicinal mushroom mold such as Inonnotus-obliquus, Cordyceps sinensis, Paecilomyces japonica , Tricholoma matsutake, phellinus linteus and Ganoderma lucidum. have.

More specifically, when yeast is used as a microorganism, it has a constant concentration (about 10 ⁵ to 10 ⁷ cells / mL) The first extracts of green beans, green beans, mushrooms, jasper, perennials, bingo, myrrh, jade, jade, jogu, bloom, perennial, castor, bogal, hodo, zucchini, hoicho, safflower, Yeast is inoculated into the basic medium (YM medium) containing each of them, and cultured for 30 to 120 hours while maintaining the conditions of pH 5.5 to 6.5, temperature 25 to 35 DEG C, aeration amount 0.05 to 0.5 VVM. Thereafter, the fermentation broth is centrifuged to remove the polymer precipitate and the yeast body, and the concentrate is concentrated under reduced pressure at 60 ° C in a distillation apparatus equipped with a cooling condenser, followed by lyophilization, thereby obtaining each of the fermentation extracts of medicinal plants.

When lactobacillus is used as a microorganism, it has a constant concentration (about 10 ⁵ to 10 ⁷ cells / mL) The primary culture medium containing the primary extracts of each of the first extracts of japonica, japonica, bingoja, japonica, yestae, japonica, jogu, bloom, baldness, castor, MRS medium) and cultured for 24 to 120 hours under the conditions of pH 6.5 to 7.5, temperature 35 to 38 ° C, aeration amount 1 VVM. Thereafter, the fermentation broth is centrifuged to remove the polymer precipitate and the lactic acid bacterium, and the concentrate is concentrated under reduced pressure at 60 ° C in a distilling apparatus equipped with a cooling condenser, followed by lyophilization, thereby obtaining each of the fermentation extracts of medicinal plants.

When bifidobacteria are used as microorganisms, they have a constant concentration (about 10 ⁵ to 10 ⁷ cells / mL), and they are found in the order of Hwanggi, A primary culture medium containing the first extracts of red liquor, laquat culture, white liquorice, bingo liquor, white waxy tea, yuqiu, jogu chrysanthemum, (BL medium) inoculated with bifidobacteria and cultured for 24 to 120 hours at pH 6.5 to 7.5, temperature 35 to 38 ° C, and anaerobic culture conditions. Thereafter, the fermentation broth is centrifuged to remove the polymer precipitate and bifidus microbial cells, and the suspension is concentrated in a distillation apparatus equipped with a cooling condenser at 60 ° C and freeze-dried to obtain the respective fermentation extracts of medicinal products.

In addition, when fungi is used as a microorganism, it is possible to use fungi such as Hwanggi, Hwaseongcho, Banks, Woinin, Skimmer mushroom, Hwangbyeong, Nonfabric, Aloe, Quercy, Birch, Rose, Kidney bean, 1 to 10% by weight of fungi are inoculated in each of the powdered pulverized product itself, or the powdered pulverized product itself, or the pulverized leaf, , Solid fermentation is carried out at 18 to 25 DEG C and 60 to 70% of humidity for 60 days to obtain each fermented extract of the medicinal substance.

The above medicinal fermentation extracts exhibit remarkably superior promoting effect on collagen synthesis when they are treated with human fibroblasts as compared with general medicinal plant extracts. In addition, when the cosmetic composition is manufactured using the above fermented extracts of medicinal materials, it is safe to the skin without any side effects when applied to human skin, and exhibits excellent skin wrinkles and wound healing effects.

The medicinal fermentation extract obtained as described above is preferably contained in the cosmetic composition for improving wrinkles of the present invention in an amount of 0.0001 to 10% by weight, more preferably 0.0005 to 10% by weight. When the content is less than 0.0001% by weight, no remarkable effect can be expected. When the content is more than 10% by weight, there is no evidence of a marked effect due to the increase of the content.

The formulation of the cosmetic composition for improving wrinkles containing the above fermented extract of the present invention as an active ingredient is not particularly limited and may be appropriately selected according to the purpose. For example, the cosmetic composition of the present invention may be formulated into ointment for external use, softening longevity, nutritional lotion, nutritional cream, massage cream, essence, pack, emulsion, oil gel and the like. In this case, the ointment for external use on skin may be prepared so as to contain 50 to 97% by weight of vaseline and 0.1 to 5% by weight of polyoxyethylene oleyl-ether phosphate in addition to the medicinal fermentation extract of the present invention. 1 to 10% by weight of polyhydric alcohols (propylene glycol, glycerin, etc.) and 0.05 to 2% by weight of a surfactant (polyethylene oleyl ether, polyoxyethylene hardened castor oil, etc.). The nutritional lotion and nutritional cream may contain 5 to 20% by weight of oils (squalane, petrolatum, octyldodecanol, etc.) and 3 to 15% by weight of wax components (cetanol, stearyl alcohol, %, And the essence can be prepared by adding 5 to 30% by weight of polyhydric alcohols such as glycerin, propylene glycol and the like in addition to the fermented extract of the present invention. The massage cream is prepared by adding 30 to 70% by weight of an oil such as liquid paraffin, petrolatum, isononylisononanoate and the like in addition to the medicinal fermented extract of the present invention. In addition to the fermented extract of the present invention, the pack contains polyvinyl alcohol A peel off pack or a general emulsifiable cosmetic composition containing a weight% of a pharmacologically active ingredient of the present invention is characterized in that the pharmaceutical composition of the present invention contains a 5 to 30 wt% pigment such as kaolin, talc, zinc oxide, titanium dioxide, ) Pack.

In addition, the cosmetic composition for wrinkle improvement of the present invention may contain, in addition to the above-mentioned ingredients, the ingredients such as oil, water, a surfactant, a moisturizer, a lower alcohol, a thickener, a chelating agent, a dye, a preservative, Can be appropriately mixed and used as needed.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following examples.

[Example]

Example 1

Bamboo leaves, aloe vera, chestnut, birch, rose, kidney bean, oyster mushroom, japanese mushroom, japanese ginseng, 200 g of each ground product obtained by purchasing and crushing castor oil, horsetail, pomace, hodo, pumpkin, hoioceros, safflower, persimmon and persimmon leaves was added to 3 L of purified water or 80% methanol, and the mixture was refluxed for 3 hours Lt; / RTI > After filtration through a 100-mesh filter cloth, the residue was extracted one more time by the same method. Each of the extracts was combined and filtered through a filter paper of Whatman No. 2 at room temperature to remove insoluble matters. The extract was concentrated under reduced pressure at 60 ° C in a distillation apparatus equipped with a cooling condenser, followed by lyophilization, ≪ / RTI > Table 1 below shows the dry weight of the extract per 100 g of medicament according to the extraction solvent.

Medicinal 80% methanol Purified water Hwanggi 6.2 g 5.5 g Sophora 6.7 g 6.1 g Bank 4. g 3.8 g Wisdom 8.7 g 7.6 g Skirt mushroom 8.6 g 7.8 g Yellowish white 6.2 g 5.3 g Non-leaf 5.1 g 4.3 g Aloe 8.6 g 8.1 g Julie 3.5 g 2.8 g Birch 5.5 g 4.6 g rose 4.3 g 3.6 g Kangnamgok 14.2 g 11.7 g Oyster mushroom 7.9 g 6.5 g Laxification 4.3 g 3.7 g Centipede 6.6 g 5.2 g Bingo 5.4 g 4.6 g Mushroom 4.7 g 4.0 g Virtue tree 6.3 g 5.3 g Jade 5.1 g 4.1 g Chow 4.8 g 3.3 g Be bored 6.2 g 5.0 g Prosperity 4.3 g 3.5 g Palma Christi 13.9 g 11.1 g Blood 4.1 g 3.1 g Department 5.2 g 4.5 g Wodo 6.3 g 5.1 g pumpkin 7.6 g 6.1 g Hoi Cho 3.2 g 2.4 g Safflower 4.2 g 3.1 g Hwangje 7.7 g 5.1 g Persimmon leaf 3.1 g 2.6 g

Examples 2 to 32

Each of the herbal extracts obtained using 80% methanol was added to 5% by weight of YM medium as a basic medium for Saccharomyces cerevisiae, which was a yeast, and fermented.

Specifically, Saccharomyces cerevisiae was inoculated into a yeast basic medium (YM medium) containing 5 wt% of each herbal extract at a concentration of about 10 ⁵ to 10 ⁷ cells / mL, Lt; 0 > C, and aeration amount of 0.05-0.5 VVM. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and the yeast organism, and then concentrated under reduced pressure at 60 DEG C in a distilling apparatus equipped with a cooling condenser, followed by freeze-drying to obtain a final fermented yeast fermentation product. The dry weight of the dried fermented product of the medicinal yeast is shown in Table 2 below.

Medicinal Dry weight of fermentation broth (g) Medicinal Dry weight of fermentation broth (g) Example 2 (Hwangjung) 6.11 Example 18 (Rhizome) 6.13 Example 3 (Rhizome) 6.20 Example 19 (Goodyard) 6.08 Example 4 (Bank) 5.97 Example 20 (jade) 5.97 Example 5 (Native) 5.99 Example 21 (Jochuu) 6.25 Example 6 (Skirt mushroom) 6.05 Example 22 (Beware) 6.10 Example 7 (yellowish white) 6.13 Example 23 (Herbalage) 6.02 Example 8 (non-papillary) 5.98 Example 24 (Castor) 5.99 Example 9 (Aloe) 6.16 Example 25 (hemoglobin) 5.86 Example 10 (muffle) 6.05 Example 26 (No.) 6.19 Example 11 (Birch) 6.02 Example 27 (Hodo) 5.77 Example 12 (rose) 6.01 Example 28 (Amber) 6.00 Example 13 (Gangnamkong) 5.99 Example 29 (Hoi-Cho) 6.08 Example 14 (Oyster mushroom) 6.01 Example 30 (Safflower) 5.95 Example 15 (lyophilization) 6.12 Example 31 (Hwangjeong) 5.92 Example 16 (perennial) 5.99 Example 32 (Persimmon leaf) 6.14 Example 17 (bingo character) 6.00

Examples 33 to 63

Each of the herbal extracts obtained by using 80% methanol was added to 5% by weight of MRS medium as a basic medium for Lactobacillus acidophilus, and fermented.

Specifically, Lactobacillus acidophilus was inoculated into a lactic acid bacterial basic medium (MRS medium) containing 5 wt% of each of the herbal extracts at a concentration of about 10 ⁵ to 10 ⁷ cells / mL, and then pH 6.5 to 7.5 and temperature 35 to 38 , And a ventilation amount of 1 VVM was maintained for 48 hours. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and the lactic acid bacteria, followed by concentration under reduced pressure at 60 DEG C in a distilling apparatus equipped with a cooling condenser, followed by lyophilization to obtain a final fermented product of lactic acid bacteria. The dry weight of the fermented product of the medicinal lactic acid bacteria is shown in Table 3 below.

Medicinal Dry weight of fermentation broth (g) Medicinal Dry weight of fermentation broth (g) Example 33 (Hwangki group) 6.06 Example 49 (Rhizome) 6.08 Example 34 (Rhizome) 6.24 Example 50 (Duckwood) 5.99 Example 35 (Bank) 6.01 Example 51 (jade) 5.82 Example 36 (Native) 5.83 Example 52 (Correction) 6.03 Example 37 (skimmed mushroom) 6.11 Example 53 (Bleed) 6.02 Example 38 (yellowish white) 5.06 Example 54 (Herbalage) 5.91 Example 39 (non-foliar) 6.06 Example 55 (Castor) 6.09 Example 40 (Aloe) 6.04 Example 56 (hemoglobin) 6.11 Example 41 (Yulpie) 5.97 Example 57 (Pharmacopoeia) 6.12 Example 42 (Birch) 6.13 Example 58 (Hodo) 5.90 Example 43 (rose) 6.01 Example 59 (Amber) 6.17 Example 44 (Gangnam-gu) 5.94 Example 60 (Hoi-Cho) 6.13 Example 45 (oyster mushroom) 6.04 Example 61 (Safflower) 6.08 Example 46 (lyophilization) 6.06 Example 62 (Hwangjeong) 6.05 Example 47 (perennial) 6.10 Example 63 (Persimmon leaf) 6.05 Example 48 (bingo character) 6.01

Examples 64 to 94

Each of the herbal extracts obtained by using 80% methanol was added to the BL medium as a basic medium for bifidobacter bifidobacterium longum at 5 wt% and fermented.

Specifically, Bifidobacterium longum was inoculated into a lactic acid bacterial basic medium (BL medium) containing 5% by weight of each of the herbal extracts at a concentration of about 10 ⁵ to 10 ⁷ cells / mL, and then pH 6.5-7.5, temperature 35-38 ℃, and anaerobic culture conditions. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and bifidus microbial cells. The microbial precipitate and bifidus microbial cells were removed by a distillation apparatus equipped with a cooling condenser, followed by concentration under reduced pressure at 60 DEG C and freeze-drying to obtain a final fermented bifidus microorganism bacterium. The dry weight of the dried fermented product of the medicinal bifidus bacterium is shown in Table 4 below.

Medicinal Dry weight of fermentation broth (g) Medicinal Dry weight of fermentation broth (g) Example 64 (Hwangki group) 5.98 Example 80 (seed wormwood) 6.02 Example 65 (Rhizome) 6.02 Example 81 (Goodyard) 6.15 Example 66 (Bank) 6.12 Example 82 (jade) 5.78 Example 67 (Native) 6.02 Example 83 (crude oil) 6.20 Example 68 (skimmed mushroom) 5.82 Example 84 (tiredness) 6.08 Example 69 (yellowish white) 6.00 Example 85 (Ammonia) 5.99 Example 70 (non-foliar) 6.04 Example 86 (Castor) 6.03 Example 71 (Aloe) 6.10 Example 87 (blood yellow) 6.03 Example 72 (ufpy) 6.88 Example 88 (Pharmacopoeia) 6.03 Example 73 (birch) 6.21 Example 89 (Hodo) 6.01 Example 74 (rose) 6.15 Example 90 (Amber) 6.12 Example 75 (Gangnam-gu) 6.00 Example 91 (Hoi-Cho) 6.06 Example 76 (Oyster mushroom) 6.10 Example 92 (Safflower) 6.00 Example 77 (lyophilization) 6.04 Example 93 (Hwangjeong) 6.16 Example 78 (perennial) 6.05 Example 94 (Persimmon leaf) 6.03 Example 79 (Binders) 6.08

Examples 95 to 125

The medicinal materials used in Table 1 were sectioned and added with 5% by weight of fungi for solid fermentation.

Specifically, 200 g of each of the medicinal materials was used as a slice, Paecilomyces japonica was inoculated at 5% by weight under constant humidity, and the mixture was fermented for 60 days at a temperature of 18 to 25 캜 and a humidity of 60 to 70% g was added to 3 L of 80% methanol, and the mixture was boiled for 3 hours in a reflux condenser equipped with a condenser. After filtration with a 100 mesh filter cloth, the residue was extracted one more time by the same method. Each of the extracts was combined and filtered through a filter paper of Whatman No. 2 at room temperature to remove insoluble matter. The extract was concentrated under reduced pressure at 60 ° C in a distillation apparatus equipped with a cooling condenser and then lyophilized to obtain a final fermented product of the medicinal fungus. The dry weight of the dried fermented product of the medicinal fungus is shown in Table 5 below.

Medicinal Dry weight of fermentation broth (g) Medicinal Dry weight of fermentation broth (g) Example 95 (Hwangki group) 5.84 Example 111 (seed wormwood) 4.22 Example 96 (Rhizome) 6.31 Example 112 (Yedok tree) 5.89 Example 97 (Bank) 4.17 Example 113 (jade) 4.83 Example 98 (Native) 8.78 Example 114 (Jochuu) 4.63 Example 99 (skimmed mushroom) 7.88 Example 115 (bred) 5.77 Example 100 (yellowish white) 5.85 Example 116 (Herbalage) 4.11 Example 101 (non-foliar) 4.80 Example 117 (Castor) 11.34 Example 102 (Aloe) 7.99 Example 118 (hemoglobin) 3.78 Example 103 (ufpy) 3.34 Example 119 (Pharmacopoeia) 4.90 Example 104 (birch) 5.12 Example 120 (Hodo) 5.93 Example 105 (rose) 4.03 Example 121 (Amber) 6.21 Example 106 (Gangnam-gu) 12.31 Example 122 (Hoi-Cho) 3.11 Example 107 (Oyster mushroom) 7.31 Example 123 (Safflower) 3.34 Example 108 (lyophilization) 4.13 Example 124 (Hwangjeong) 7.13 Example 109 (perennial) 6.12 Example 125 (Persimmon leaf) 2.82 Example 110 (bingo character) 5.12

EXPERIMENTAL EXAMPLE 1: Promoting collagen synthesis

Each of the herbal extracts obtained by using 80% methanol in Example 1 and the herbal fermentation extracts of Examples 2 to 125 were added to a culture solution of human-derived fibroblasts to examine the effect of collagen synthesis at the cellular level. The synthesized collagen was quantitated using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immunoassay KIT).

The medicinal fermentation extracts of Examples 2 to 110 were added to a culture medium of human fibroblast cells (human fibroblast cells) at a final concentration of 0.005%, and cultured for 1 day. Then, the culture broth was taken and cultured with PICP EIA Kit The degree of collagen synthesis was measured at 450 nm using a spectrophotometer. For the comparison of the effects, the degree of collagen synthesis was measured in the same manner as in the case of the culture medium of the fibroblasts to which nothing had been added (control group) and the vitamin C added to the final concentration of 0.005%.

The amount of collagen production was measured by UV absorbance, and the rate of increase of collagen production was calculated as a ratio of the amount of collagen production relative to the control. The results are summarized in Tables 6 to 10 below.

sample Amount of collagen production Growth rate (%) Control group (no addition) 1.118 + 0.030 - YM medium (final concentration 0.005%) 1.202 + 0.031 107.51 MRS medium (final concentration 0.005%) 1.215 + 0.041 108.68 BL medium (final concentration 0.005%) 1.223 + 0.043 109.39 Hwanggi
(Final concentration: 0.005%) The extract of Example 1 1.346 ± 0.056 120.40 The yeast fermented extract of Example 2 1.731 + 0.043 154.92 The fermented extract of lactic acid bacteria of Example 33 1.612 + 0.040 144.19 The bifidobacterial fermentation extract of Example 64 1.607 + 0.078 143.74 The fungal fermentation extract of Example 95 1.814 + 0.055 152.25 Sophora
(Final concentration: 0.005%) The extract of Example 1 1.293 + 0.077 115.65 The fermented yeast extract of Example 3 1.674 ± 0.067 149.73 The fermented extract of lactic acid bacteria of Example 34 1.583 + 0.049 141.59 The bifidobacterial fermentation extract of Example 65 1.645 + 0.045 147.14 The fungal fermentation extract of Example 96 1.589 + 0.034 142.13 Bank
(Final concentration: 0.005%) The extract of Example 1 1.371 + 0.078 122.63 The fermented yeast extract of Example 4 1.782 + 0.037 159.39 The fermented extract of lactic acid bacteria of Example 35 1.607 + 0.025 143.74 The bifidobacterial fermentation extract of Example 66 1.756 ± 0.057 157.07 The fungal fermentation extract of Example 97 1.666 ± 0.056 149.02 Wisdom
(Final concentration: 0.005%) The extract of Example 1 1.345 + 0.040 120.30 The fermented yeast extract of Example 5 1.598 + 0.067 142.93 The fermented extract of lactic acid bacteria of Example 36 1.534 + 0.024 137.21 The bifidobacterial fermentation extract of Example 67 1.656 + 0.067 148.12 The fungal fermentation extract of Example 98 1.645 + 0.076 147.14 Skirt mushroom
(Final concentration: 0.005%) The extract of Example 1 1.284 + 0.045 114.85 The yeast fermented extract of Example 6 1.645 + 0.045 147.14 The fermented extract of lactic acid bacteria of Example 37 1.660 + 0.048 148.48 The bifidobacterial fermentation extract of Example 68 1.583 + - 0.056 141.59 The fungal fermentation extract of Example 99 1.687 + 0.048 158.50 Yellowish white
(Final concentration: 0.005%) The extract of Example 1 1.302 + 0.049 116.46 The yeast fermented extract of Example 7 1.678 ± 0.071 150.09 The fermented extract of lactic acid bacteria of Example 38 1.605 + 0.047 143.56 The bifidobacterial fermentation extract of Example 69 1.745 + 0.073 156.08 The fungal fermentation extract of Example 100 1.691 + 0.060 151.16

sample Amount of collagen production Growth rate (%) Non-leaf
(Final concentration: 0.005%) The extract of Example 1 1.378 + 0.041 123.26 The yeast fermented extract of Example 8 1.627 + 0.038 145.23 The fermented extract of lactic acid bacteria of Example 39 1.773 + 0.069 158.59 The bifidobacterial fermentation extract of Example 70 1.871 + 0.098 167.35 The fungal fermentation extract of Example 101 1.781 + 0.037 159.30 Aloe
(Final concentration: 0.005%) The extract of Example 1 1.361 + 0.073 121.74 The yeast fermented extract of Example 9 1.702 + 0.038 152.24 The fermented extract of lactic acid bacteria of Example 40 1.690 + 0.040 151.16 The bifidobacterial fermentation extract of Example 71 1.726 + 0.048 154.38 The fungal fermentation extract of Example 102 1.709 + 0.068 152.86 Julie
(Final concentration: 0.005%) The extract of Example 1 1.389 + 0.046 124.24 The yeast fermented extract of Example 10 1.772 + 0.046 158.50 The fermented extract of lactic acid bacteria of Example 41 1.805 + 0.076 161.45 The bifidobacterial fermentation extract of Example 72 1.749 ± 0.056 156.44 The fungal fermentation extract of Example 103 1.706 + 0.066 152.59 Birch
(Final concentration: 0.005%) The extract of Example 1 1.333 + 0.041 119.23 The fermented yeast extract of Example 11 1.677 ± 0.056 150.00 The fermented extract of lactic acid bacteria of Example 42 1.804 + 0.034 161.36 The bifidobacterial fermentation extract of Example 73 1.709 + 0.066 152.86 The fungal fermentation extract of Example 104 1.596 + 0.043 142.75 rose
(Final concentration: 0.005%) The extract of Example 1 1.304 + 0.040 116.61 The fermented yeast extract of Example 12 1.883 + 0.064 168.43 The fermented extract of lactic acid bacteria of Example 43 1.800 + 0.073 161.00 The bifidobacterial fermentation extract of Example 74 1.714 + 0.080 153.31 The fungal fermentation extract of Example 105 1.778 + 0.087 159.03 Kangnamgok
(Final concentration: 0.005%) The extract of Example 1 1.368 + 0.062 122.36 The fermented yeast extract of Example 13 1.756 + 0.064 157.07 The lactic acid bacteria fermented extract of Example 44 1.790 + 0.030 160.11 The bifidobacterial fermentation extract of Example 75 1.688 + 0.031 150.98 The fungal fermentation extract of Example 106 1.750 + - 0.058 156.53 Oyster mushroom
(Final concentration: 0.005%) The extract of Example 1 1.400 + 0.060 125.22 The fermented yeast extract of Example 14 1.761 + 0.055 157.51 The fermented extract of lactic acid bacteria of Example 45 1.834 + 0.072 164.04 The bifidobacterial fermentation extract of Example 76 1.739 + 0.073 155.55 The fungal fermentation extract of Example 107 1.902 + 0.063 170.13 Laxification
(Final concentration: 0.005%) The extract of Example 1 1.371 ± 0.059 122.63 The yeast fermented extract of Example 15 1.683 + 0.070 150.54 The fermented extract of lactic acid bacteria of Example 46 1.699 + 0.038 151.97 The bifidobacterial fermentation extract of Example 77 1.734 0.049 150.89 The fungal fermentation extract of Example 108 1.831 + 0.048 155.10 Centipede
(Final concentration: 0.005%) The extract of Example 1 1.274 + - 0.051 113.95 The yeast fermentation extract of Example 16 1.724 0.060 154.20 The fermented extract of lactic acid bacteria of Example 47 1.729 + 0.038 154.65 The bifidobacterial fermentation extract of Example 78 1.671 + 0.082 149.55 The fungal fermentation extract of Example 109 1.720 + 0.034 153.85

sample Amount of collagen production Growth rate (%) Bingo
(Final concentration: 0.005%) The extract of Example 1 1.369 + 0.036 122.45 The yeast fermented extract of Example 17 1.771 + 0.050 158.41 The fermented extract of lactic acid bacteria of Example 48 1.708 ± 0.052 157.77 The bifidobacterial fermentation extract of Example 79 1.612 + 0.030 144.18 The fungal fermentation extract of Example 110 1.754 + 0.048 156.89 Mushroom
(Final concentration: 0.005%) The extract of Example 1 1.371 + 0.060 122.63 The yeast fermented extract of Example 18 1.808 + 0.071 161.72 The fermented extract of lactic acid bacteria of Example 49 1.770 + 0.037 158.31 The bifidobacterial fermentation extract of Example 80 1.680 + - 0.052 159.93 The fungal fermentation extract of Example 111 1.730 0.089 150.27 Virtue tree
(Final concentration: 0.005%) The extract of Example 1 1.274 + 0.048 113.95 The yeast fermentation extract of Example 19 1.625 + 0.075 145.35 The fermented extract of lactic acid bacteria of Example 50 1.567 + 0.047 140.16 The bifidobacterial fermentation extract of Example 81 1.624 + 0.037 145.26 The fungal extract of Example 112 1.772 + 0.036 158.50 Jade
(Final concentration: 0.005%) The extract of Example 1 1.338 + 0.038 119.68 The yeast fermented extract of Example 20 1.571 + 0.048 140.52 The fermented extract of lactic acid bacteria of Example 51 1.672 + 0.040 149.55 The bifidobacterial fermentation extract of Example 82 1.631 + 0.068 145.89 The fungal fermentation extract of Example 113 1.707 + 0.062 152.68 Chow
(Final concentration: 0.005%) The extract of Example 1 1.387 + 0.040 124.06 The fermented yeast extract of Example 21 1.658 ± 0.069 148.30 The fermented extract of lactic acid bacteria of Example 52 1.783 + 0.038 159.48 The bifidobacterial fermentation extract of Example 83 1.609 + 0.048 143.92 The fungal fermentation extract of Example 114 1.641 + 0.033 146.78 Be bored
(Final concentration: 0.005%) The extract of Example 1 1.401 + 0.055 125.31 The yeast fermented extract of Example 22 1.770 + 0.071 158.31 The fermented extract of lactic acid bacteria of Example 53 1.725 + 0.048 154.29 The bifidobacterial fermentation extract of Example 84 1.800 + 0.097 161.00 The fungal fermentation extract of Example 115 1.876 + 0.081 167.80 Prosperity
(Final concentration: 0.005%) The extract of Example 1 1.338 + 0.037 119.68 The fermented yeast extract of Example 23 1.706 + 0.061 152.59 The fermented extract of lactic acid bacteria of Example 54 1.771 + - 0.052 158.41 The bifidobacterial fermentation extract of Example 85 1.699 + 0.082 151.97 The fungal fermentation extract of Example 116 1.750 + 0.048 156.53 Palma Christi
(Final concentration: 0.005%) The extract of Example 1 1.277 + 0.055 114.22 The yeast fermented extract of Example 24 1.591 + - 0.053 142.31 The fermented extract of lactic acid bacteria of Example 55 1.745 + 0.064 156.08 The bifidobacterial fermentation extract of Example 86 1.667 ± 0.057 149.11 The fungal extract of Example 117 1.501 + 0.072 134.26 Blood
(Final concentration: 0.005%) The extract of Example 1 1.340 + 0.036 119.86 The fermented yeast extract of Example 25 1.834 + 0.066 164.04 The fermented extract of lactic acid bacteria of Example 56 1.735 + 0.035 155.19 The bifidobacterial fermentation extract of Example 87 1.887 ± 0.059 168.78 The fungal fermentation extract of Example 118 1.741 + - 0.052 155.72

sample Amount of collagen production Growth rate (%) Department
(Final concentration: 0.005%) The extract of Example 1 1.361 + - 0.052 121.74 The yeast fermented extract of Example 26 1.656 + 0.038 148.12 The fermented extract of lactic acid bacteria of Example 57 1.720 + 0.030 153.85 The bifidobacterial fermentation extract of Example 88 1.787 ± 0.051 159.84 The fungal fermentation extract of Example 119 1.565 + 0.063 139.98 Wodo
(Final concentration: 0.005%) The extract of Example 1 1.222 + 0.034 109.30 The yeast fermented extract of Example 27 1.672 + 0.039 149.55 The fermented extract of lactic acid bacteria of Example 58 1.638 + 0.072 146.51 The bifidobacterial fermentation extract of Example 89 1.701 + - 0.058 152.15 The fungal fermentation extract of Example 120 1.803 + 0.071 161.27 pumpkin
(Final concentration: 0.005%) The extract of Example 1 1.360 + 0.074 121.65 The fermented yeast extract of Example 28 1.745 + 0.024 156.08 The fermented extract of lactic acid bacteria of Example 59 1.640 + 0.062 146.69 The bifidobacterial fermentation extract of Example 90 1.702 + 0.064 152.24 The fungal fermentation extract of Example 121 1.779 + 0.055 159.12 Hoi Cho
(Final concentration: 0.005%) The extract of Example 1 1.339 + 0.066 119.77 The yeast fermented extract of Example 29 1.600 + 0.037 143.11 The fermented extract of lactic acid bacteria of Example 60 1.683 + 0.042 150.54 The bifidobacterial fermentation extract of Example 91 1.668 ± 0.070 149.19 The fungal fermentation extract of Example 122 1.649 ± 0.075 147.50 Safflower
(Final concentration: 0.005%) The extract of Example 1 1.372 + 0.035 122.72 The fermented yeast extract of Example 30 1.670 + 0.026 149.37 The fermented extract of lactic acid bacteria of Example 61 1.786 ± 0.054 159.75 The bifidobacterial fermentation extract of Example 92 1.756 + 0.079 157.07 The fungal fermentation extract of Example 123 1.649 + 0.076 147.50 Hwangje
(Final concentration: 0.005%) The extract of Example 1 1.237 + 0.046 110.64 The fermented yeast extract of Example 31 1.558 + 0.055 139.36 The lactic acid bacteria fermented extract of Example 62 1.607 + 0.047 143.74 The bifidobacterial fermentation extract of Example 93 1.631 + 0.069 145.89 The fungal fermentation extract of Example 124 1.665 ± 0.053 148.93 Persimmon leaf
(Final concentration: 0.005%) The extract of Example 1 1.319 ± 0.057 117.98 The fermented yeast extract of Example 32 1.666 ± 0.059 149.02 The fermented extract of lactic acid bacteria of Example 63 1.703 + 0.043 152.33 The bifidobacterial fermentation extract of Example 94 1.742 + 0.047 155.81 The fungal fermentation extract of Example 125 1.882 + 0.055 168.34 Vitamin C (final concentration 0.0005%) 1.528 + 0.075 136.67

As shown in Tables 6 to 9, the extracts of fermented medicinal materials obtained according to Examples 2 to 125 exhibited remarkably increased production of collagen in human fibroblasts as compared with the extracts using 80% methanol in Example 1, Considering that it was not purified at all, it was found that fibroblasts had a strong ability to promote collagen synthesis as compared with vitamin C, which is a known promoter of collagen synthesis.

EXPERIMENTAL EXAMPLE 2. Promotion of Collagen Synthesis by Concentration

The human fibroblasts cultured in the same manner as in Experimental Example 1 were adjusted to 0.5%, 0.05%, 0.005%, and 0.0005%, respectively, of the hull fungus fermentation extract of Example 95 to obtain collagen synthesis And the results are shown in Table 10 below.

sample Application concentration (%) Melanin production Inhibition rate (%) Control group (no addition) - 1.118 + 0.030 - The fermented hwanggi fungus extract of Example 95 Final concentration (0.0005%) 1.613 + 0.048 144.28 Final concentration (0.005%) 1.814 + 0.055 162.25 Final concentration (0.05%) 1.870 + 0.044 167.26 Final concentration (0.5%) 1.994 + 0.069 178.80 Vitamin C Final concentration (0.0001%) 1.528 + 0.075 172.00

As shown in Table 10, it was confirmed that the fermented extract of Huangfu fungus has very strong collagen synthesis promoting ability against cultured human-derived fibroblasts. Although the fermented extract of Hwanggi mildew is not a single substance but a mixed substance, it has the ability of promoting collagen synthesis equal to or higher than that of vitamin C, which is a single substance, and the fermented hwanggi-fermented extract of the present invention shows no cytotoxicity even at a concentration of 0.05% It was predicted that it could be very useful as a skin wrinkle remover or a wound healing agent because it has strong collagen synthesis promoting ability.

Example 126 and Comparative Example 1

The ointment extracts of Example 95 were used to prepare external ointments for skin using the prescription of Table 11 below.

division Example 126 Comparative Example 1 Hwanggi mold fermentation extract 10 - Diethyl sebacate 8 8 Nonsense 5 5 Polyoxyethylene oleyl ether phosphate 6 6 Sodium benzoate Suitable amount Suitable amount vaseline To 100 To 100

Example 127 and Comparative Example 2

Using the fermented mugwort extract of Example 95, the cream was prepared according to the prescription of Table 12 below.

division Example 127 Comparative Example 2 Hwanggi mold fermentation extract 1.0 - Stearic acid 15.0 15.0 Cetanol 1.0 1.0 Potassium hydroxide 0.7 0.7 glycerin 5.0 5.0 Propylene glycol 3.0 3.0 antiseptic Suitable amount Suitable amount incense Suitable amount Suitable amount Purified water To 100 To 100

Example 128 and Comparative Example 3

Using the fermented mugwort fermented extract of Example 95, the softening longevity was prepared according to the prescription shown in Table 13 below.

division Example 128 Comparative Example 3 Hwanggi mold fermentation extract 0.2 - ethanol 10.0 10.0 Polyoxyethylene sorbitan polylauric acid 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 glycerin 5.0 5.0 1,3-butylene glycol 6.0 6.0 incense Suitable amount Suitable amount Pigment Suitable amount Suitable amount Purified water To 100 To 100

Example 129 and Comparative Example 4

Nutritional lotion was prepared using the fermented extract of Yuka Fungus of Example 95 as shown in Table 14 below.

division Example 129 Comparative Example 4 Hwanggi mold fermentation extract 0.1 - Vaseline 2.0 2.0 Sesquioleic acid sorbitan 0.8 0.8 Polyoxyethylene oleylethyl 1.2 1.2 Methyl paraoxybenzoate Suitable amount Suitable amount Propylene glycol 5.0 5.0 ethanol 3.2 3.2 Carboxyvinyl polymer 18.0 18.0 Potassium hydroxide 0.1 0.1 Pigment Suitable amount Suitable amount incense Suitable amount Suitable amount Purified water To 100 To 100

Example 130 and Comparative Example 5

Using the fermented mushroom fermentation extract of Example 95, a pack was prepared according to the prescription of Table 15 below.

division Example 130 Comparative Example 5 Hwanggi mold fermentation extract 0.5 - glycerin 5.0 5.0 Propylene glycol 4.0 4.0 Polyvinyl alcohol 15.0 15.0 ethanol 8.0 8.0 Polyoxyethylene oleylethyl 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 incense Suitable amount Suitable amount Pigment Suitable amount Suitable amount Purified water To 100 To 100

Example 131 and Comparative Example 6

Using the fermented mushroom fermentation extract of Example 95, the essence was prepared by the prescription of Table 16 below.

division Example 131 Comparative Example 6 Hwanggi mold fermentation extract 5.0 - Propylene glycol 10.0 10.0 glycerin 10.0 10.0 Sodium hyruronate aqueous solution (1%) 5.0 5.0 ethanol 5.0 5.0 Polyoxyethylene hardened castor oil 1.0 1.0 Methyl paraoxybenzoate 0.1 0.1 incense Suitable amount Suitable amount Purified water To 100 To 100

Experimental Example  3. Effect of wound healing by collagen synthesis

Five - week - old male rats were tested for wound healing using a tensile strength method that reflects the quality and quantity of wound regeneration in incisions.

First, hair was removed from the back of the rat, incised with a scalpel and sutured. The creams of Example 127 and Comparative Example 2 were administered to the incision sites once a day for 6 days. Six days after the administration, the rats used in the experiment were slaughtered and the skin of the wound area was extracted. Three 1-cm pieces of skin having a width of 1 cm orthogonal to the wire line were prepared for each individual and rupture tension was measured with a rheometer. The measured tensile strength (g / cm) was taken as an index of the strength of regenerated collagen fiber. The increase rate of tensile strength was represented by the relative strength at 100% of the tensile strength of the control group to which ethanol alone was applied without addition of the sample. The results of the strength measurements are summarized in Table 17 below.

division Tensile strength (g / cm) Growth rate (%) Example 127 549 100 Comparative Example 2 671 122

Experimental Example 4. Effect of improving wrinkles

A panel test was conducted on the skin wrinkle reducing effect using the creams of Example 127 and Comparative Example 2.

First, 30 healthy women from 35 to 50 years old were divided into two groups by 15 persons, the cream of Comparative Example 2 was applied to Group 1, the cream of Example 127 was applied to the face portion once a day for 3 months Respectively. After 3 months, the improvement of skin wrinkles was evaluated by the questionnaire survey. As to the improvement of the skin wrinkles and the elasticity of the subject, the three steps of no improvement, slight improvement and significant improvement as compared with before use were determined, and the results are shown in Table 18 below.

division No improvement Slight improvement A significant improvement Example 127 2 7 6 Comparative Example 2 7 7 One

As shown in Table 18, when the cream of Example 127 according to the present invention was used, it was found that the effect of improving skin wrinkles and improving elasticity was excellent.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments. It will be understood that various modifications and changes may be made without departing from the scope of the appended claims.

Claims (4)

The present invention relates to a fermented milk extract,
Wherein the fermented extract of the black tea mugwort is a fermented extract obtained by culturing yeast, lactic acid bacterium or bifidus bacterium when cultured, or a fermented extract obtained by solid fermentation with fungus inoculated with black mugwort . The method according to claim 1,
Wherein the yeast is Saccharomyces cerevisiae, Schizosaccharomyces ellipsoids or Saccharomyces boulardii, Lactobacillus are Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus bulgaricus , Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus confusus, and Lactobacillus fermentum or Lactobacillus brevis, Bifidobacterium is Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum or Bifidobacterium infantis . The method according to claim 1,
Wherein the fungus is Inonnotus-obliquus, Cordyceps sinensis, Paecilomyces japonica, phellinus linteus or Ganoderma lucidum. The method according to claim 1,
Wherein the fermentation extract is contained in an amount of 0.0001 to 10% by weight in the cosmetic composition.