KR101652244B1 - Cosmetic composition for controlling anti-acne and anti-comed - Google Patents

Abstract

The present invention relates to an antimicrobial effect against propionibacterium acnes, which is known as a cause of acne, and to remove inflammation caused by clogging of the pores caused by cottonseed, Inhibiting or alleviating the effect of the present invention. The cosmetic composition for improving acne skin according to the present invention can prevent acne or improve it with excellent effect by containing a fermented extract of a herbal medicine as an active ingredient.

Acne, propionibacterium acnes, cottonseed, fermentation, herbal medicines

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic composition for improving acne skin,

The present invention relates to a cosmetic composition for improving acne skin, which contains an extract of fermented herbal medicines and has an antibacterial effect against acne causing bacteria, an inhibitory effect on sebum secretion and an anti-inflammatory effect.

Acne refers to inflammation of hair follicles, hair follicles and surrounding tissues due to the action of hormones, excess sebum secretion, bacterial infection, and the like.

The causes of acne can be divided into several types. The first cause is excessive sebum production. Sebum is secreted from the sebaceous glands, and in normal amounts, it prevents the harmful substances from outside the skin from penetrating between keratinocytes. However, in the environment of puberty, before and after menstruation, at the beginning of pregnancy, taking contraceptive pill, stress, etc., the human body shows a hormone balance different from the normal state. This altered hormone balance stimulates the sebaceous glands, which are induced by excessive secretion of sebum. It may also be genetically increased in many cases by sebaceous glands and may be increased by an increase in skin temperature due to changes in external temperature or erroneous eating habits.

The second cause is infection of pores. Excessively secreted sebum contains a lot of fatty acids, which increases the pH of the skin surface and dissolves the lipids of the skin, thereby reducing the skin's own defense. On the surface of the skin with increased pH, the causative bacteria of acne become easily proliferated, and the acne bacteria that are proliferated cause the pyogenic inflammation through weakened skin.

In general, acne can be divided into 4 stages according to symptoms. The first step is acne-causing acne that causes excess sebum to accumulate in the pores around the sebaceous glands and form a mucosa. Acne blemishes in the first step are acne lesions that are initially a mild stage, but they are not good for cosmetics. At this stage, good management can inhibit the development of inflammation. In stage 2 acne vulgaris, acne bacterium grows and inflammation reaction occurs and red acne appears. It is called papule and scarring can occur if not properly managed. In stage 3 acne, red acne inflammation reaction becomes worse, and a pus is formed inside. It is called pustule. In this stage, special treatment is needed because scars may remain after treatment. The last four-stage nodular acne is a stage in which pus pockets burst from the skin and cause more inflammation around the acne lesion. This is called a nodule. It is a period when the inflammation has penetrated into the dermis. It requires professional treatment. Can be prolonged.

The treatment of acne is topical drug application and oral medication, and surgical treatment such as laser treatment, lapping, etc.

Local topical application may include the use of antimicrobial agents such as azelaic acid and salicylic acid for the purpose of antibacterial action against propionibacterium acnes, which is known as a cause of acne, or benzoyl peroxide peroxide and the like are used as external ointment type. However, azelaic acid is difficult to formulate and salicylic acid is classified as a preservative in Korea and can not contain more than 0.5% in the formulation. Benzoyl peroxide is a raw material that can not be used as a raw material for cosmetics in Korea.

Oral drug therapy uses retinoids, corticosteroids, and other drugs to inhibit the function of sebaceous glands and inhibit hair follicle wall destruction. These preparations have side effects such as skin rash and skin dryness when taking too much dose, and allergies may occur depending on the individual, so caution is required for their use.

Considering various aspects such as the speed of the effect and economical efficiency, the problem of already onset, progressive acne and other skin diseases can be said to be more effective for the external preparation for direct use on the skin (refer to Korean Patent Publication No. 2004-0056081).

At the stage of cosmetics, there are not many substances that can show such a medical treatment effect. However, it can be used over a long period of time. Thus, many plant extracts and synthetic materials have been patented as compositions for the improvement of acne skin. However, There are few products that are safe to skin and have the desired effect because skin side effects are high as well as therapeutic effect such as skin keratinization occurs afterwards.

The present invention relates to a cosmetic composition for improving acne skin which has an antimicrobial effect against acne causing bacteria and has an effect of controlling sebum secretion essential for acne improvement and has an effect of improving acne in a wide range of stages, And to provide a composition therefor.

The cosmetic composition for improving acne skin according to the present invention is characterized by containing a fermented extract of a herbal medicine.

Further, the cosmetic composition for improving acne skin according to the present invention is characterized in that it is used for antibacterial treatment of Propionibacterium acnes which is a causative agent of acne, for regulating secretion of sebum, for decreasing the production of cottonseed, and for anti-inflammatory use do.

The herbal medicine fermented extract used in the present invention can be obtained by adding a herbal medicine to yeast or lactic acid bacteria, culturing and fermenting.

The yeast is Saccharomyces sp ., Schizosaccharomyces sp ., Torulopsis sp ., Rhodotorula sp ., or Candida sp . Can be used. In addition, the lactic acid bacteria are Lactobacillus sp ., Streptococcus sp ., Leuconostoc sp ., or Bifidobacteria sp . Can be used.

The cosmetic composition for improving acne skin according to the present invention primarily exhibits its effect by rapidly suppressing the growth and growth of microorganisms involved in skin acne and skin irritation. Secondly, the cosmetic composition for improving acne skin, Improves acne skin by suppressing sebaceous secretion and extinguishing acne blemishes. It also has anti-inflammatory effect and improves acne skin.

The cosmetic composition for improving acne according to the present invention is characterized in that it contains at least one selected from the group consisting of licorice, gobon, , Ginseng, peony, clove, sculptor, dermis, creation, silkworm, silkworm, silkworm, guinea fowl, passerine, red ginseng, coriander, hwanggi, ginseng, bank, kimono, skirt mushroom, yellowish white, unspread, aloe, silk, birch From the group consisting of green beans, oyster mushrooms, jade mushrooms, white mushrooms, bingo mushrooms, black mushrooms, yukdogi, jukje, mushrooms, As an active ingredient, a fermented extract of a herb medicine selected from the species or more.

The licorice (Glycyrrhiza glabra) is a perennial herb belonging to Legumisosae. It is used in medicinal or tea by drying in the shade. It is used for neuralgia, cough, arteriosclerosis, dysuria.

Angelica tenuissima is a perennial plant belonging to Umbelliferae and has efficacy for abdominal pain, toothache and diarrhea.

Agastache rugosa is a perennial herb that belongs to the genus Labiatae and is used in cases of gastroenteritis, sweating, fever, and dryness.

Platycodon gradiflorum is a perennial herb that belongs to the Campanulaceae and is used for cannibalism and toothache.

The Cervus nippon is a dried plucked grass of a plum or locust belonging to the deer (Cervidae) and dried to protect the liver and the ginseng and strengthen the bones and muscles.

The Angelica gigas are perennial roots belonging to the Umbelliferae and are used for body weakness, headache and constipation.

The jujube (Zizyphus jujube) is a fruit of broad-leaved arboreous tree in Rhamnaceae and is used for nursing, tanning, detoxification, shinhae, and labor.

The Cordyceps sinensis belongs to Hypocreaceae and is used for anticancer, arteriosclerosis, hypertension and heart disease.

Aucklandia lappa is the root of perennial herbaceous plants belonging to the compositae.

The Poria cocos belong to the Polypooraceae and are used for immobility, noise and diarrhea.

The white paper (Angelica dahurica) is a biennial herb belonging to the Umbelliferae. It is used for headache, dizziness, Guan Wasa.

Amomum villosum is a perennial herb that belongs to the genus Zingiberaceae and is used for vomiting, diarrhea, and early anxiety.

Thymus vulgalis is a perennial plant belonging to the family Lamiaceae and is used for headache, depression, anemia, and fatigue.

The pomegranate (Punica granatum) is a perennial deciduous broad-leaved arboreous tree belonging to the pomegranate family (Punicaceae) and is used for arteriosclerosis, diarrhea, athlete's foot, and the like.

The above-mentioned Acorus tatarinowii belongs to the genus Araceae and is used for forgetfulness, purulent tumor, bruise.

The tuber melanosporum is an underground raw mushroom of a Western tongue, and is used for edible purposes.

Tricholoma matsutake is a mushroom of bacillus subtilis. It is used for the suppression of cholesterol in blood, the promotion of blood circulation (hand, foot trembling, treatment of back and knee palsy), arteriosclerosis, heart disease, diabetes and hyperlipemia.

Ganoderma lucidum is a fruiting body of Ganoderma Lucidum and its family members belonging to Polypooraceae and is used for arteriosclerosis, neuralgia, cancer and the like.

Lindera strichnifolia is a root of Tiantai mushroom belonging to the genus Lauraceae, and has the efficacy of the bladder cold, the urine odor, the opacity, and the mid-storm.

Gentiana scabra is a perennial herb that belongs to Gentianaceae and is used for the production of swine, epilepsy, roundworms, and eczema.

Ginseng is a perennial herbaceous perennial belonging to the genus Araliaceae. It is used for blood circulation, cold circulation and bronchitis.

Paeonia albiflora is a perennial herb belonging to Ranunculaceae and is used for accelerating blood circulation, analgesia and asthma.

The clove (Eugenia caryophyllata) is a dried flower of a celery belonging to an evergreen tree belonging to the genus Myrtaceae. It is used for the treatment of facial, mouth odor, and sexual function.

The sculptor (Gleditsia sinensis) is dried in the visible part of the sculptured tree belonging to the Reguminosae, and is used for boils and insecticides.

The dermis (Citruunshiu) is a dry mandarin ripened fruit shell belonging to the Ruraceae.

The creation (Atractylodes chinensis) is a perennial herb that belongs to the compositae and is dried in the trunks of the buds, and is used for strokes, conjunctivitis, hypertension, and dizziness.

The Saussurea involucrate is a perennial herb that belongs to Ranunculaceae and is used as an analgesic, a sorghum, a cardiotonic agent, a diuretic, and the like.

Zanthoxylum piperitum is a mature periwinkle of the Sancho wood belonging to the Ruraceae, and is used for abdominal pain, diarrhea, antiparasitic action, and skin eczema.

The passerine (Prunus armeniaca) is a mature fruit seed of an apricot tree belonging to the Rosaceae family, and is used for the treatment of coughing and breathing.

Red gienseng is steamed steamed ginseng, which is used to treat arteriosclerosis, diabetes, restoration, and sexual function.

It is said that Cormus officinalis is a dried leaf of the fruit which is a deciduous tree belonging to the layered tree branch, and it is said that it has the effect of hunting and sniffing the liver and kidney in the screen of the newborn kidneys. In the present pharmacologically, , Anti-inflammation, and antibacterial activity.

Astragalus membranaceus is a perennial herb that belongs to Leguminosae, and is used for diuretics, exanthema, and tonsils.

The Houttuynia cordata is a perennial herb belonging to Saururaceae and is used for jaundice and hepatitis.

Ginkgo biloba (Ginkgo biloba) is a perennial herbaceous tree belonging to the banking family (Gingkoaceae) and is used for heart disease, hypertension, asthma and seawater.

Coix lachryma-jobi var.ma-yuen is a first-generation herb of Gramineae and is used for chronic arthritis and abortion.

The above-mentioned Schizophyllum commune belongs to the skirt mushroom family.

The Phellodendron chinense is used for the treatment of diseases caused by mushrooms, which are separated from the cork layer of the cedar wood belonging to the Rutaceae family.

Eriobotrya japonica is a dried leaf of the loquat tree belonging to Roosaceae, and is used for chronic bronchitis, seawater and vomiting.

Aloe ferox is a perennial herb that belongs to Riliaceae and is used for antibacterial, anti-ulcer, and anti-cancer. Castanea crenata is a chestnut astringent which belongs to the Fagaceae family.

The birch (Betula platyphylla) belongs to Betulaceae and is used for jaundice, diarrhea, nephritis, pulmonary tuberculosis and gastritis.

The rose (Rosa hybrida) is a shrubs of Rosaceae and is used for ornamental or fragrant.

The above-mentioned kidney bean (Phaseolus vulgaris) is an annual herb that belongs to leguminosae, and is used for medicines such as boils, diuresis, diarrhea, each edema and edema.

The Pleurotus ostreatus belongs to the Pleurotus ostreatus, and is used for edible purposes in the aged trees of hardwoods.

Camompsis grandiflora is a vine plant belonging to Bignoniaceae and is used for the treatment of diseases caused by eosinophilia or hematopoiesis.

The Opuntia ficus is also referred to as an open-handed cactus, and is used for skin diseases, rheumatism, and burns.

The bananas (Areaca catechu) refers to the fruit of the bananas belonging to the Palmae (Palmae), and is used for anthelmintics, authoritics and the like.

The Artemisia capillaries belong to the family Asteraceae (Compositae) and are used for diuretics and jaundice.

The Mallotus japonicus is a deciduous arboreous tree belonging to the main pole and is used for gastritis, gastric ulcer, and stomach cancer.

Polygonatum officinale is a perennial plant belonging to the Liliaceae family. It uses roots of divergent for diabetes, myocardial degeneration, hyperlipidemia and the like.

Incaria sinensis belongs to Rubiaceae, and is used for seizures, paralysis, and hypotension.

Tribulus terrestirs is a fruit of the southern stallion, a perennial herb belonging to Zypophyllaceae, and is often used in ophthalmic diseases.

Hamamellis japonica is a deciduous deciduous witch hazel, which has skin sedative and protective action.

The castor (Ricinus communis) is a mature seed of the mackerel, a perennial herb belonging to the Euphorbiaceae, and is used for subjugation, constipation, scab, burns.

Draconis resina is dried in the fruits and stems of the giraffins, such as perennial evergreen belonging to the Palmae family, and is used for bleeding, abrasion and physiological impurity.

Cucumis sativus is a fruit of cucumber, an annual herb belonging to the genus Cucurbutaceae.

Juglans regia is a seed of walnut belonging to the genus Juglandaceae. It is used for paddy rice, uterine bleeding, gastric hyperplasia, lower abdominal pain, acute chronic gonorrhea and the like.

The amber (Cucurbita moschata) is an annual herb that belongs to the genus Cucurutaceae and is used for edible and asthma treatment.

The Saxifraga stolonifera is a perennial herb belonging to the Saxifragaceae and is used for pertussis, burns, and statues.

The safflower (Carthamus tinctorius) is a biennial herb that belongs to the Compositae family and is used for women's diseases, abdominal pain,

Polygonatum sibiricum is a perennial herb that belongs to Liliaceae and is dried in the roots of the layered roundworm, and is used for diabetes.

The persimmon leaves (Diospyroskaki) are used for medicinal purposes by drying the leaves of persimmon belonging to Ebenaceae, and are used for the paralysis and the high blood circulation.

The herbal medicines can be prepared as a herbal medicine extract according to a conventional method, for example, extracted with an organic solvent such as distilled water, anhydrous or low-boiling alcohol having 1 to 4 carbon atoms, concentrated under reduced pressure and then dried to obtain a first extract . Specifically, there are commercially available ginseng products such as licorice, gobon, kwakyang, gyeonggyeong, antler, angelica, jujube, cordyceps, myeonbyeol, Baekbokryeong, blank, sine, safflower, pomegranate, , Peony, clove, sculptor, dermis, creation, silkworm, silkworm, silkworm, guinea fowl, passerine, red ginseng, coriander, hwanggi, ginseng, bank, woolen, skirt mushroom, yellowish white, alfalfa, birch, rose Purchase and finely crush the dried mushrooms, plum mushrooms, plum blossoms, blooming plums, bingo chrysanthemums, mud worms, yew trees, 5 to 20 volumes of water or 1 to 4 carbon atoms in an organic solvent such as anhydrous or hydrous lower alcohol in an extractor equipped with a reflux condenser is heated at 50 to 100 DEG C for 1 to 5 hours to the dry weight of the pulverized material, . After filtration with a filter cloth, the residue is further extracted one or more times by the same method. The extracts are combined, concentrated under reduced pressure, and lyophilized or spray dried to obtain a dried extract.

The herbal medicine extracts obtained as described above may be added to yeast or lactic acid bacteria and cultured to obtain a final herbal medicine fermented extract. At this time, it is preferable that the herbal medicine extract is added to the basic medium of yeast or lactic acid bacteria in an amount of 1 to 20% by weight.

More specifically, when yeast is used as a microorganism, it has a constant concentration (about 10 ⁵ to 10 ⁷ cells / mL) Ginseng, clove, clove, sculptor, dermis, creation, silkworm, silkworm, chrysanthemum, guinea fowl, passerine, red ginseng, coriander, hwanggi, ginseng, bank Mushroom, white mushroom, white mushroom, alfalfa, aloe, chestnut, birch, rose, kidney bean, oyster mushroom, (YM medium) containing the primary extracts of the leaves, horseradish, amber, hoiocerum, safflower, hanjang, and persimmon leaf, and then the yeast was inoculated with the yeast and cultured at pH 5.5 to 6.5, temperature 25 to 35 ° C, aeration amount 0.05 to 0.5 And cultured for 30 to 120 hours while maintaining the condition of VVM. Thereafter, the fermentation broth is centrifuged to remove the polymer precipitate and the yeast strain, and the suspension is concentrated in a distillation apparatus equipped with a cooling condenser at 60 ° C and freeze-dried to obtain each herbal extract.

When lactobacillus is used as a microorganism, it has a constant concentration (about 10 ⁵ to 10 ⁷ cells / mL), and there is a low concentration of liquorice, gobon, Ginseng, mushroom, clove, mulberry, dermis, creation, chrysanthemum softening, chrysanthemum, guerrilla, passerine, red ginseng, coriander, hwanggi, ginseng, bank, womans, skirt mushroom , Yellowish white, alfalfa, aloe, chestnut, birch, rose, kidney bean, oyster mushroom, Lactic acid bacteria were inoculated in a basic medium (MRS medium) containing each of the first extracts of Hoiicho, safflower, hanjang, and persimmon leaves, followed by incubation at pH 6.5-7.5, temperature 35-38 ° C, For 120 hours. Thereafter, the fermentation broth is centrifuged to remove the polymer precipitate and the lactic acid bacterium, and the concentrate is concentrated under reduced pressure at 60 ° C in a distillation apparatus equipped with a cooling condenser, followed by lyophilization, thereby obtaining each herbal fermentation extract.

The fermented extract of herbal medicines thus obtained is preferably contained in an amount of 0.0001 to 10% by weight, preferably 0.0005 to 10% by weight, more preferably 5 to 10% by weight, in the cosmetic composition for acne improvement of the present invention . When the content is less than 0.0001% by weight, no remarkable effect can be expected. When the content is more than 10% by weight, there is no evidence of a marked effect due to the increase of the content.

The formulation of the cosmetic composition for the improvement of acne containing the fermented extract of herbal medicines according to the present invention as an active ingredient is not particularly limited and may be appropriately selected according to the purpose. For example, the cosmetic composition of the present invention may be formulated into ointment for external use, softening longevity, nutritional lotion, nutritional cream, massage cream, essence, pack, emulsion, oil gel and the like. In this case, the external skin ointment may be prepared so as to contain 50 to 97% by weight of vaseline and 0.1 to 5% by weight of polyoxyethylene oleyl-ether phosphate in addition to the herbal medicine fermentation extract of the present invention. 1 to 10% by weight of polyhydric alcohols (propylene glycol, glycerin, etc.) and 0.05 to 2% by weight of a surfactant (polyethylene oleyl ether, polyoxyethylene hardened castor oil, etc.) in addition to the fermented extract. The nutritional lotion and nutritional cream may contain 5 to 20 wt% of oils (squalane, petrolatum, octyldodecanol, etc.) and 3 to 15 wt% of wax components (cetanol, stearyl alcohol, beeswax, etc.) %, And the essence can be prepared by containing 5 to 30% by weight of polyhydric alcohols such as glycerin, propylene glycol and the like in addition to the herbal medicine fermented extract of the present invention. The massage cream is prepared by adding 30 to 70% by weight of an oil such as liquid paraffin, petrolatum, isononylisononanoate, etc. in addition to the herbal medicine fermentation extract of the present invention. In addition to the fermented extract of herbal medicine of the present invention, A peel off pack or a general emulsifiable cosmetic composition containing 5 to 30% by weight of a pigment such as kaolin, talc, zinc oxide and titanium dioxide in addition to the fermented extract of the present invention, ) Pack.

In addition, the cosmetic composition for improving acne according to the present invention may contain, in addition to the above-described ingredients, the ingredients such as oil, water, a surfactant, a moisturizer, a lower alcohol, a thickener, a chelating agent, a dye, a preservative, Can be appropriately mixed and used as needed.

[Example 1] Production of fermented herbal medicine extract

Dried licorice, Gobon, Kwakyeong, Gyungyang, Antler, Angelica, Jujube, Cordyceps, Myeonbyeol, Baekbokryeong, Baekji, Sine, Sephinum, Pomegranate, Seokchangpo, Truffle mushroom, Songyu, Yeongji, Ohje, Yongdam, Ginseng, Peony, Cloves After finishing three times, it was pulverized with a homomixer and stirred to obtain a fine powder phase. The pulverized herbal medicine was again pulverized with an atomizer (USA) and passed through a screen (pie 0.8 mm, ROUND HALL). After repeating this process, the mixture was passed through a ROUND HALL having a diameter of 0.3 mm and then sieved with a sieve (standard body, 200 mesh) to obtain a superfine powdery phase. The obtained herbal powder was added to 1.5 L of purified water so as to have a concentration of 3% by weight, and was adjusted to a proper pH (5.0 to 7.5) using sodium hydroxide as a basic composition, such as carbon source, nitrogen source, phosphate salt, I did it.

The culture medium was supplemented with Sacchromyces S. cerevisiae was inoculated into a basic medium containing 10 ⁶ cells / mL of herbal powder and cultured for 36 hours at pH 5.5 to 6.5, temperature 25 to 35 ° C, aeration amount 0.05 to 0.5 VVM.

The fermentation broth was centrifuged at 10,000 × g for 30 minutes to obtain a final fermentation broth extract of herbaceous material from which the polymer precipitate and the lactic acid bacteria were removed.

[Example 2] Production of fermented herbal medicine extract

Except that the herbal medicines are sculptors, dermis, creation, silkworms, silkworms, guinea fowl, passersby, red ginseng, coriander, hwanggi, ginseng, bank, womans, skirt mushrooms, yellowish white, alfalfa, birch, And was prepared in the same manner as in Example 1.

[Example 3] Production of fermented herbal medicine extract

Except that the herbal medicines were oyster mushroom, japanese mushroom, Paekcheolgi, Bingoljae, Rhododendron, Yeokdeok, Jokcheong, Jogu, 1. ≪ / RTI >

[Comparative Example 1] Preparation of herbal medicine extract

Ginseng, peony, clove, ginseng, ginseng, ginseng, safflower, pomegranate, ginseng, ginseng, ginseng, ginseng, Dandelion, chrysanthemum, duckweed, aloe, chestnut, birch, rose, kidney bean, oyster mushroom, japanica After drying, it is put into distilled water as an extracting solvent, and the herbal medicine (herb medicine) is added to the herb medicine. The extract was extracted so as to be 30% by weight. After cooling for 3 days, the mixture was filtered with a 0.2 ㎛ filter to prevent the formation of a precipitate. Ultrasonic herbal extracts were prepared by UV light for 3 days for sterilization.

Hereinafter, preferred formulations are shown to facilitate the understanding of the present invention, but the following formulations are only illustrative of the present invention, and the scope of the present invention is not limited to the following formulation examples.

[Formulation Example 1-3 and Comparative Formulation Example 1-3]

Formulation Examples 1 to 3 and Comparative Formulation Examples 1 to 4 were prepared according to the ingredients and the contents (% by weight) shown in Table 1 below. Specifically, Formulation Example 1 is an effective ingredient of acne skin improvement (C phase in the following Table 1), including licorice, gobon, wako, gakyung, antler, angelica, jujube, (Example 1) of the composition of the present invention was prepared by blending the extract of the present invention with a fermented extract of pomegranate, pomegranate, pomegranate, truffle, pine mushroom, ganoderma, (Example 2) of a fermented extract of Pseudomonas sp., Bombyx mori, Rhododendron, Rhododendron, Red ginseng, Sansui oil, Hwanggi, Fermented extract of fermented mushroom, japanese mushroom, japanese mushroom, bamboo jelly, bingo japonica, japonica, oriental japonica, japonica, . Also, Comparative Formulation Example 1 contained no effective ingredient for acne skin improvement, Comparative Formulation Example 2 contained a reference substance for antibacterial activity and contained clindamycin, which is widely used as a therapeutic agent for acne, Example 3 contained an unfermented herbal extract (Comparative Example 1).

The manufacturing methods of Formulation Examples 1 to 3 and Comparative Formulation Examples 1 to 4 are as follows. The components of the phase A in Table 1 were completely dissolved, the components of the phase B were completely dissolved in a separate melting tank, and the phase B was added to the phase A to be mixed and solubilized. The components of the C phase were added thereto in accordance with the mixing ratios shown in Table 1, followed by mixing and homogenization, followed by filtration to prepare the compositions.

division Formulation Example 1 Formulation Example 2 Formulation Example 3 Comparative Formulation Example 1 Comparative Formulation Example 2 Comparative Formulation Example 3 A Deionized Water TO 100 TO 100 TO 100 TO 100 TO 100 TO 100 EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 glycerin 5.0 5.0 5.0 5.0 5.0 5.0 B ethanol 5.0 5.0 5.0 5.0 5.0 5.0 PEG-60 hydrogenated castor oil 0.4 0.4 0.4 0.4 0.4 0.4 Perfume 0.04 0.04 0.04 0.04 0.04 0.04 C Example 1 5.0 - - - - - Example 2 - 5.0 - - - - Example 3 - - 5.0 - - - Comparative Example 1 - - - - 5.0 - Clindamycin - - - - - 5.0

[Experimental Example 1: Test for antibacterial activity against acne bacteria]

Each of the cosmetic compositions prepared in the formulations of Formulation Examples 1 to 3 and Comparative Formulation Examples 1 to 3 was tested for antibacterial activity against Propionibacterium acnes <ATCC 6919: medium-BHI broth> .

The test method for the antimicrobial activity against acne bacteria was as follows.

(1) Preparation of test strain

Propionibacterium acnes were cultured in an anaerobic culture inoculated with BHI broth.

(2) Dilution solution preparation

0.15 ml of the above test solution was added to 15 ml of BHI broth (pH 6.8) or LB broth (pH 4.5), and the mixture was used as a diluting solution.

(3) Preparation of sample

The raw cosmetic compositions prepared from Formulation Examples 1 to 3 and Comparative Formulation Examples 1 to 3 were directly used as a sample.

(4) Antimicrobial activity test

1) In a 96 well plate (96 well plate), add the sample to the starting concentration and set the total volume to 200 μl with dilution solution. Add 100 ul of dilution solution to the remaining wells.

2) Thoroughly mix the mixture of line 1, then take 100ul and mix well. Mix well. Then, take 100ul and add to the third row. Double dilution is done.

3) After culturing at 32 ° C for 24 hours and 48 hours, determine the degree of bacterial growth by the degree of suspension, and determine the minimum concentration without bacterial growth as the MIC (Minimun Inhibitory concentration) value. If the mixture is opaque and it is difficult to judge the growth of the microorganism, check with a microscope.

The results of the antimicrobial activity tests against acne bacteria are shown in Table 2 below.

Item pH Propionibacterium acnes Formulation Example 1 5.6 &Lt; 5 ppm Formulation Example 2 5.3 <1 ppm Formulation Example 3 5.2 &Lt; 5 ppm Comparative Formulation Example 1 5.7 * Not measurable Comparative Formulation Example 2 5.5 <10 ppm Comparative Formulation Example 3 5.7 <30 ppm

Formulation Examples 1 to 3 had an antibacterial activity of MIC 20 ppm to 1 ppm against the test bacteria. The lower the ppm concentration in the MIC, the more effective it is against acne.

[Experimental Example 2: Improvement of acne and reduction of sebum secretion and test for irritation]

Twelve individuals with acne were allowed to use the cosmetic compositions prepared from Formulation Examples 1 to 3 and Comparative Formulation Examples 1 to 3 for one month. Acne improvement scale is from 1 point to 5 points, 1 point is 'No', 3 points are 'Normal' and 5 points are 'Very agree'. The experimental results are shown in the following Table 3 as the average score of 12 persons.

The acne extermination period was based on the number of days of disappearance readings, and the results were based on the results after 1 month with or without recurrence of acne. The reduction of sebaceous secretion was from 1 point to 5 points, 1 point was 'No', 3 points were 'Normal' and 5 points were 'Very agree'. Experimental results are shown in Table 3 below with an average score of 12 persons. The presence or absence of skin irritation was evaluated as (number of stimuli) / (total number of test subjects).

Inflammatory acne
Improving Acne blemish
Time of extinction Recurrence of acne Decrease sebaceous glands Presence or absence of stimulation Formulation Example 1 4.2 5 days radish 4.0 1/12 Formulation Example 2 4.5 2 days radish 4.1 2/12 Formulation Example 3 4.4 5 days radish 4.0 1/12 Comparative Formulation Example 1 2.1 13th U 2.0 0/12 Comparative Formulation Example 2 4.2 2 days radish 4.1 9/12 Comparative Formulation Example 3 3.0 10 days U 2.8 1/12

As shown in Table 3, Formulations 1 to 3 showed no recurrence of acne compared to Comparative Formulations 1 and 3, and an average of 4 points or more for inflammatory acne improvement and an average of 5 days or less for acne- Acquired more than 4 points on the reduction of secretion, and it shows that there is an excellent effect on acne improvement. On the other hand, Comparative Formulation Example 2 containing the antibacterial activity standard substance showed similar efficacy to Formulation Examples 1 to 3, but the stimulation was stronger than that of the other experimental examples in use, so that there was a fear of skin irritation have.

Therefore, it can be seen that the fermented extract of herbal medicines is suitable for patients with acne skin when various factors such as irritation rate, improvement rate, degree of improvement and degree of decrease of sebum secretion are considered.

[Experimental Example 3: Inflammation improvement effect]

The antiinflammatory effect was evaluated by the inhibitory effect of prostaglandin production. The effect of the extracted extracts on macrophages was measured. First, aspirin was added to the macrophage collected from the abdominal cavity of mice to a final concentration of 500M to irreversibly inhibit the cyclooxygenase ("COX") activity remaining in the cells. Then, 100 μl of the electric suspension was added to each well of a 96-well cell culture tube and cultured for 2 hours in an incubator under the conditions of 5% CO ₂ and 37 ° C. to adhere macrophages to the surface of the container. The adherent macrophages were then washed three times with PBS and used for the efficacy of the extract. The RPMI medium containing 1% (w / v) LPS was added to 5x10 4 cells / ml of the electrophoretic macrophages and cultured for 12 hours to induce the production of prostaglandin. 100 μl of the extract was treated to release the liberated prostaglandin Assay (ELISA).

At this time, the inhibitory activity of the extract on prostaglandin production was determined by setting the difference of the prostaglandins produced in the LPS-treated group and the untreated group to 100%, and treating the LPS and the sample together to determine the percentage of reduced prostaglandin And the results (inhibitory effect on the production of prostaglandins) are shown in Table 4 below.

Blank 100% Control group (aspirin treatment group) 25.0% Formulation Example 1 32.8% Formulation Example 2 34.2% Formulation Example 3 39.2% Comparative Formulation Example 1 15.4% Comparative Formulation Example 2 24.2% Comparative Formulation Example 3 25.5%

As shown in Table 4, it was found that the effect of inhibiting the production of prostaglandin by Formulation Examples 1 to 3 was much higher than that of the control group treated with aspirin, and that the extract of the herbal medicine without fermentation (Comparative Formulation Example 3) It was found that the effect of inhibiting the production of prostaglandins by Formulation Examples 1 to 3 was high.

Claims (11)

A mixture of herbal medicines made of carvings, dermis, creation, silkworm, silkworm, silkworm, passerine, red ginseng, coriander, hwanggi, ginseng, bank, kimono, skirt mushroom, yellowish white, alfalfa, birch, As an active ingredient, a cosmetic composition for improving acne skin, The fermentation is carried out using yeast Saccharomyces cerevisiae , Wherein the content of the fermentation extract is 5 to 10% by weight based on the total weight of the composition. delete delete The cosmetic composition for improving acne skin according to claim 1, wherein the content of the fermented extract is 5 wt% with respect to the total weight of the composition. delete delete The cosmetic composition for improving acne skin according to claim 1, wherein the composition is antimicrobial to Propionibacterium acnes causing acne. The cosmetic composition for improving acne skin according to claim 1, wherein the composition regulates sebum secretion. 2. The cosmetic composition for improving acne skin according to claim 1, wherein the composition reduces skin formation. The cosmetic composition for improving acne skin according to claim 1, wherein the composition exhibits an anti-inflammatory effect. The cosmetic composition for improving acne skin according to claim 1, wherein the composition has a formulation of a softening agent, a nutritional lotion, a nutritional cream, a massage cream, a pack, a gel, a lipstick, a makeup base or a foundation.