KR101566820B1 - Cosmetic composition containing moisturizing complex for moisturizing the skin - Google Patents

Abstract

The present invention relates to a cosmetic composition for moisturizing skin, and more particularly, to a cosmetic composition for skin moisturizing, which comprises a fermented extract of herbal medicines obtained by culturing yeast or lactic acid bacteria, And to a cosmetic composition excellent in moisturizing effect.

Fermentation, herbal medicine, moisturizing

Description

[0001] The present invention relates to a cosmetic composition for moisturizing skin,

The present invention relates to a cosmetic composition for skin moisturizing which can improve skin dryness caused by aging, and more particularly, to a cosmetic composition for moisturizing skin, which comprises a fermented extract of a herb medicine obtained by culturing yeast or lactic acid bacteria with a herbal medicine, To a cosmetic composition having excellent skin moisturizing effect.

 The skin is divided into three layers of epidermis, dermis and subcutaneous adipose tissue in order from the outside, and functions to protect the human body from physical and chemical stimulation of the external environment. Especially, skin carries about 65 ~ 70% of the human body's moisture, that is, various physiologically active substances necessary for the human body, and helps to keep the skin soft and moist, It plays a role. The epidermis is divided into the stratum corneum, granular layer, the superficial layer and the basal layer in the order from the outside. The cells of the stratum corneum act like bricks, and the intercellular lipids between the keratinocytes function as the mortar, (J. Invest. Dermatol. 80 (Suppl.), 44-49, 1983). Recently, it has been newly found that not only the water content in the stratum corneum is increased but also the differentiation process developed from the keratinocytes in the basal layer to the keratinocytes in the outermost stratum corneum can be performed normally to function as a skin moisture retention barrier lost. In other words, physiological skin drying results in prolonged removal time of the stratum corneum, lowering lipid synthesis ability of epidermal cells, and decreasing the amount of moisturizing factors and lipids in the stratum corneum of the skin. Therefore, by promoting differentiation of keratinocytes, . Therefore, it can be expected that an excellent skin moisturizing effect can be obtained by using a water retaining material in the stratum corneum and a substance promoting keratinization promotion together.

In recent years, there has been remarkable use of herbal medicines in the field of cosmetics, however, herbal medicinal compositions using herbal medicinal extracts extracted by ordinary methods have not only a desired skin moisturizing effect but also have a long practical time to obtain such extracts. I did.

On the other hand, the fermentation broth extract does not use any other solvent, so the sugar content in the herbal medicine is changed into alcohol, and after fermentation and aging process, the extraction rate of active ingredient such as flavonoid, vitamin C, vitamin P, amino acid and catechin contained in the herbal medicine And thus, a much better skin moisturizing effect can be obtained.

Therefore, it is necessary to develop a new skin moisturizer which is stable without skin irritation through the extract of the herbal medicine fermentation broth, and maximizes the skin moisturizing effect.

It is an object of the present invention to provide a cosmetic composition for non-irritating, skin-safe and skin-moisturizing cosmetic composition which is free from the problem of inducing skin disease and exhibits its effect in a short time, and is long-lasting and highly safe.

In order to achieve the above object, the present invention provides a cosmetic composition for moisturizing, which comprises as an active ingredient, a fermented extract of herbal medicines obtained by adding and cultivating a herb medicine to yeast or lactic acid bacteria.

The cosmetic composition containing the fermented extract of herbal medicines according to the present invention has an excellent effect on moisturizing the skin. In particular, the cosmetic composition of the present invention uses a fermented extract of herbal medicines, promotes the differentiation of the horny layer of skin cells, restores the barrier function of damaged skin, And can be usefully used as a cosmetic composition for moisturizing

Hereinafter, the present invention will be described in detail.

As a result of applying the extract of the herbal medicine fermentation broth obtained by fermenting the herbal medicines to the cosmetic composition, the present inventors have found that the extraction rate of the effective ingredient such as flavonoid, vitamin C, vitamin P, amino acid and catechin contained in the herbal medicine becomes remarkably high, The effect is excellent, the skin is safe to human skin, and the present invention is completed based on this.

The cosmetic composition for moisturizing of the present invention is characterized by containing a fermented extract of a herbal medicine as an active ingredient.

The herbal medicine fermented extract used in the present invention can be obtained by adding a herbal medicine to yeast or lactic acid bacteria, culturing and fermenting.

The yeast is Saccharomyces sp ., Schizosaccharomyces sp ., Torulopsis sp ., Rhodotorula sp ., or Candida sp . Can be used. In addition, the lactic acid bacteria are Lactobacillus sp ., Streptococcus sp ., Leuconostoc sp ., or Bifidobacteria sp . Can be used.

The cosmetic composition for moisturizing according to the present invention is characterized in that it contains at least one selected from the group consisting of licorice, gobon, kwakyang, gyeonggyeong, antler, angelica, jujube, cordyceps, As an active ingredient, a fermented extract of a herb medicine selected from the group consisting of ginseng, peony, clove, sculptor, dermis, creation, silkworm, silkworm,

The licorice (Glycyrrhiza glabra) is a perennial herb belonging to Legumisosae. It is used in medicinal or tea by drying in the shade. It is used for neuralgia, cough, arteriosclerosis, dysuria. The herbivore (Angelica tenuissima) is a multicellular herb that belongs to the mountain type (Umbelliferae) and has efficacy for abdominal pain, toothache and diarrhea. Agastache rugosa is a perennial herb that belongs to the genus Labiatae and is used in cases of gastroenteritis, sweating, fever, and dryness. Platycodon gradiflorum is a perennial herb that belongs to the Campanulaceae and is used for cannibalism and toothache. The Cervus nippon is a dried plucked grass of a plum or locust belonging to the deer (Cervidae) and dried to protect the liver and the ginseng and strengthen the bones and muscles. The Angelica gigas are perennial roots belonging to the Umbelliferae and are used for body weakness, headache and constipation. The jujube (Zizyphus jujube) is a fruit of broad-leaved arboreous tree in Rhamnaceae and is used for nursing, tanning, detoxification, shinhae, and labor. The Cordyceps sinensis belongs to Hypocreaceae and is used for anticancer, arteriosclerosis, hypertension and heart disease. Aucklandia lappa is the root of perennial herbaceous plants belonging to the compositae. The Poria cocos belong to the Polypooraceae and are used for immobility, noise and diarrhea. The white paper (Angelica dahurica) is a biennial herb belonging to the Umbelliferae. It is used for headache, dizziness, Guan Wasa. Amomum villosum is a perennial herb that belongs to the genus Zingiberaceae and is used for vomiting, diarrhea, and early anxiety. Thymus vulgalis is a perennial plant belonging to the family Lamiaceae and is used for headache, depression, anemia, and fatigue. The pomegranate (Punica granatum) is a perennial deciduous broad-leaved arboreous tree belonging to the pomegranate family (Punicaceae) and is used for arteriosclerosis, diarrhea, athlete's foot, and the like. The above-mentioned Acorus tatarinowii belongs to the genus Araceae and is used for forgetfulness, purulent tumor, bruise. The tuber melanosporum is an underground raw mushroom of a Western tongue, and is used for edible purposes. Tricholoma matsutake is a mushroom of bacillus subtilis. It is used for the suppression of cholesterol in blood, the promotion of blood circulation (hand, foot trembling, treatment of back and knee palsy), arteriosclerosis, heart disease, diabetes and hyperlipemia. Ganoderma lucidum is a fruiting body of Ganoderma Lucidum and its family members belonging to Polypooraceae and is used for arteriosclerosis, neuralgia, cancer and the like. Lindera strichnifolia is a root of Tiantai mushroom belonging to the genus Lauraceae, and has the efficacy of the bladder cold, the urine odor, the opacity, and the mid-storm. Gentiana scabra is a perennial herb that belongs to Gentianaceae and is used for the production of swine, epilepsy, roundworms, and eczema. Ginseng is a perennial herbaceous perennial belonging to the genus Araliaceae. It is used for blood circulation, cold circulation and bronchitis. Paeonia albiflora is a perennial herb belonging to Ranunculaceae and is used for accelerating blood circulation, analgesia and asthma. The clove (Eugenia caryophyllata) is a dried flower of a celery belonging to an evergreen tree belonging to the genus Myrtaceae. It is used for the treatment of facial, mouth odor, and sexual function. The sculptor (Gleditsia sinensis) is dried in the visible part of the sculptured tree belonging to the Reguminosae, and is used for boils and insecticides. The dermis (Citruunshiu) is a dry mandarin ripened fruit shell belonging to the Ruraceae. The creation (Atractylodes chinensis) is a perennial herb that belongs to the compositae and is dried in the trunks of the buds, and is used for strokes, conjunctivitis, hypertension, and dizziness. The Saussurea involucrate is a perennial herb that belongs to Ranunculaceae and is used as an analgesic, a sweet potato, a cardiotonic agent, a diuretic, and the like. Zanthoxylum piperitum is a mature periwinkle of the Sancho wood belonging to the Ruraceae, and is used for abdominal pain, diarrhea, antiparasitic action, and skin eczema. The passerine (Prunus armeniaca) is a mature fruit seed of an apricot tree belonging to the Rosaceae family, and is used for the treatment of coughing and breathing. Red gienseng is steamed steamed ginseng, which is used to treat arteriosclerosis, diabetes, restoration, and sexual function.

The herbal medicines can be prepared as a herbal medicine extract according to a conventional method, for example, extracted with an organic solvent such as distilled water, anhydrous or low-boiling alcohol having 1 to 4 carbon atoms, concentrated under reduced pressure and then dried to obtain a first extract . This study was conducted to investigate the effects of various antioxidant drugs such as licorice, Gobon, Kwanghyang, Gyeonggyeong, Dongguk, Angelica gigas, Jujube, Cordyceps sinensis, Ginseng, peony, clove, sculptor, dermis, creation, silkwool, silkworm, silkworm, rootstock, passerine or red ginseng are finely pulverized, and 5 to 20 parts by volume of water, Or an organic solvent such as a functional lower alcohol is heated at 50 to 100 ° C for 1 to 5 hours in an extractor equipped with a reflux condenser to extract an extract of herbal medicines. After filtration with a filter cloth, the residue is further extracted one or more times by the same method. The extracts are combined, concentrated under reduced pressure, and lyophilized or spray dried to obtain a dry extract.

The herbal medicine extracts obtained as described above may be added to yeast or lactic acid bacteria and cultured to obtain a final herbal medicine fermented extract. At this time, it is preferable that the herbal medicine extract is added to the basic medium of yeast or lactobacillus at 1 to 20% by weight.

The yeast is Saccharomyces sp ., Schizosaccharomyces sp ., Torulopsis sp ., Rhodotorula sp ., or Candida sp . Can be used. In addition, the lactic acid bacteria are Lactobacillus sp ., Streptococcus sp ., Leuconostoc sp ., or Bifidobacteria sp . Can be used.

More specifically, when yeast is used as a microorganism, it has a constant concentration (about 10 ⁵ to 10 ⁷ cells / mL) , First extract of red ginseng, persimmon, persimmon, ginseng, red ginseng, red ginseng, pine ginseng, seokchonpo, truffle mushroom, mulberry, ganoderma, omega, yongdam, ginseng, peony, clove, Yeast is inoculated in the basic medium (YM medium) and cultured for 30 to 120 hours under conditions of pH 5.5 to 6.5, temperature 25 to 35 ° C, aeration amount 0.05 to 0.5 VVM. Thereafter, the fermentation broth is centrifuged to remove the polymer precipitate and the yeast strain, and the suspension is concentrated in a distillation apparatus equipped with a cooling condenser at 60 ° C and freeze-dried to obtain each herbal extract.

When lactobacillus is used as a microorganism, it has a constant concentration (about 10 ⁵ to 10 ⁷ cells / mL), and there is a low concentration of liquorice, gobon, (MRS medium) containing the first extracts of red ginseng, red ginseng, red ginseng, red ginseng, red ginseng, tsushinpu, truffle, tsuyu, , And cultured for 24 to 120 hours under the conditions of pH 6.5 to 7.5, temperature 35 to 38 占 폚, aeration amount 1 VVM. Thereafter, the fermentation broth is centrifuged to remove the polymer precipitate and the lactic acid bacterium, and the concentrate is concentrated under reduced pressure at 60 ° C in a distillation apparatus equipped with a cooling condenser, followed by lyophilization, thereby obtaining each herbal fermentation extract.

The fermented extract of herbal medicines obtained as described above is preferably contained in an amount of 0.0001 to 10% by weight, more preferably 0.0005 to 10% by weight, in the cosmetic composition for moisturizing of the present invention. When the content is less than 0.0001% by weight, no remarkable effect can be expected. When the content is more than 10% by weight, there is no evidence of a marked effect due to the increase of the content.

The formulation of the moisturizing cosmetic composition containing the fermented extract of herbal medicines according to the present invention as an active ingredient is not particularly limited and may be appropriately selected according to the purpose. For example, the cosmetic composition of the present invention may be formulated into ointment for external use, softening longevity, nutritional lotion, nutritional cream, massage cream, essence, pack, emulsion, oil gel and the like. In this case, the ointment for external use on skin may be prepared to contain 50 to 97% by weight of vaseline and 0.1 to 5% by weight of polyoxyethylene oleyl-ether phosphate in addition to the herbal medicine fermentation extract of the present invention. 1 to 10% by weight of polyhydric alcohols (propylene glycol, glycerin, etc.) and 0.05 to 2% by weight of a surfactant (polyethylene oleyl ether, polyoxyethylene hardened castor oil, etc.). The nutritional lotion and nutritional cream may contain 5 to 20 wt% of oils (squalane, petrolatum, octyldodecanol, etc.) and 3 to 15 wt% of wax components (cetanol, stearyl alcohol, beeswax, etc.) %, And the essence can be prepared by containing 5 to 30% by weight of polyhydric alcohols such as glycerin, propylene glycol and the like in addition to the herbal medicine fermented extract of the present invention. The massage cream is prepared by adding 30 to 70% by weight of an oil such as liquid paraffin, petrolatum, isononylisononanoate, etc. in addition to the herbal medicine fermentation extract of the present invention. In addition to the fermented extract of herbal medicine of the present invention, A peel off pack or a general emulsifiable cosmetic composition containing 5 to 30% by weight of a pigment such as kaolin, talc, zinc oxide and titanium dioxide in addition to the fermented extract of the present invention, ) Pack.

In addition, the cosmetic composition for moisturizing of the present invention may further contain, in addition to the above-described components, the oil, water, surfactant, humectant, lower alcohol, thickener, chelating agent, coloring agent, preservative, And may be appropriately used in accordance with necessity.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following examples.

[Example 1] Production of fermented herbal medicine extract

Ginseng, peony, clove, ginseng, ginseng, safflower, pomegranate, ginseng, ginseng, ginseng, ginseng, ginseng, Sculptor, dermis, creation, vulcanization, silkworm, persimmon, persimmons and red ginseng were finely ground, and then ground and stirred with a homomixer to obtain a fine powder phase. The pulverized herbal medicine was again pulverized with an atomizer (USA) and passed through a screen (pie 0.8 mm, ROUND HALL). After repeating this process, the mixture was passed through a ROUND HALL having a diameter of 0.3 mm and then sieved with a sieve (standard body, 200 mesh) to obtain a superfine powdery phase. The obtained herbal powder was added to 1.5 L of purified water so as to have a concentration of 3% by weight, and was adjusted to a proper pH (5.0 to 7.5) using sodium hydroxide as a basic composition, such as carbon source, nitrogen source, phosphate salt, I did it.

The culture medium was supplemented with Sacchromyces S. cerevisiae was inoculated into a basic medium containing 10 ⁶ cells / mL of herbal powder and cultured for 36 hours at pH 5.5 to 6.5, temperature 25 to 35 ° C, aeration amount 0.05 to 0.5 VVM.

The fermentation broth was centrifuged at 10,000 × g for 30 minutes to obtain a final fermentation broth extract of herbaceous material from which the polymer precipitate and the lactic acid bacteria were removed.

[Comparative Example 1] Preparation of herbal medicine extract

Ginseng, peony, clove, ginseng, ginseng, ginseng, safflower, pomegranate, ginseng, ginseng, ginseng, ginseng, The extracts were extracted with 30% by weight of distilled water by adding distilled water as an extraction solvent. After cooling for 3 days, the mixture was filtered with a 0.2 ㎛ filter to prevent the formation of a precipitate. Ultrasonic herbal extracts were prepared by UV light for 3 days for sterilization.

[Experimental Example 1] Differentiation inducing effect in keratinocytes

The following experiments were conducted to examine the differentiation inducing effects of keratinocytes in the extracts of fermented herbal medicines and herbal medicine extracts.

First, human keratinocytes were put into a 96-well microtiter plate containing KGM (Keratinocyte Growth Media) medium containing 1.0% fetal bovine serum at a concentration of 5,000 cells / well, And then cultured in KGM medium without fetal bovine serum and grown to 70 ~ 80%. The cultured human keratinocytes were placed in a culture flask and grown to about 80%.

Then, 200 μl of each of the fermented extract of herb medicine of Example 1 and the herbal medicine extract of Comparative Example 1 was treated for 4 days, and then the protein was dissolved by mixing a urea, a reducing agent such as SDS and β-mercaptoethanol, Respectively. The remaining horny layer was then measured for peptide concentration at 310 nm and the absorbance values were compared. At this time, the low-concentration calcium (0.05 mM) treatment group without the extract treatment was used as a negative control group, and the high calcium concentration treatment group (1.5 mM) treatment not treated with the extract was used as a positive control group.

As a result, the proliferation effect of keratinocytes was 72% in Example 1 and 34% in Comparative Example 1 as shown in Table 1 below. As a result, it can be seen that the effect of inducing keratinocyte differentiation is enhanced in the herbal medicine fermentation extract rather than the herbal medicine extract.

[Table 1]

division Growth effect (%) Example 1 72 Comparative Example 1 34 Negative control group 0 Positive control group 100

[Experimental Example 2] Skin barrier restorative effect

Extracts of fermented herbal medicines and herbal medicine extracts In order to investigate the effect of restoring skin barrier in experimental animals, the following experiment was performed. At this time, the skin barrier recovery effect was measured by changing the skin moisture loss (TEWL: TransEpidermal Water Loss, also referred to as transdermal water loss) with the damaged barrier state as 100. The skin water loss refers to the water supplied from the skin's dermis It diffuses to the upper part of the stratum corneum mainly by passive diffusion and is lost to the outside.

First, acetone was repeatedly applied to young female hairless mice (Charles River Co., Ltd.) that had passed 8-10 weeks after birth to impair barrier function, and then TEWL (transdermal water loss) was measured by a water loss meter as measured by (evaporimeter EP1, ServoMED) to 4.0 g / m ² / is reached h each extract (example 1 saengyakjae fermentation extract, saengyakjae extract of Comparative example 1) ² area 5 cm for a sample containing the The degree of restoration of skin barrier function was evaluated by evaluating the degree of decrease of skin moisture loss after application, and measurements were performed after 1 hour, 2 hours, 4 hours and 8 hours. In addition, a group not containing the extract was used as a negative control, and a known lipid mixture (ceramide: cholesterol: fatty acid = 2: 1: 1) was used as a positive control.

The skin barrier recovery effect was measured by changing the skin moisture loss (TEWL) with the damaged barrier state being 100 as described above, and the results are shown in Table 2 below. As shown in the following Table 2, Example 1 (fermented extract of herbal medicines) showed almost similar recovery effect as compared with the positive control group.

[Table 2]

division After 1 hour After 2 hours After 4 hours After 8 hours Example 1 61.3 54.2 46.8 30.5 Comparative Example 1 72.8 64.5 58.9 48.6 Negative control group 89.4 91.4 86.7 84.6 Positive control group 68.1 45.8 36.1 32.4

[Experimental Example 3] Effect of skin moisturizing effect through human body experiment

Extracts of fermented herbal medicines and herbal medicine extracts In order to evaluate the effect of increasing the skin moisturizing power through the human body experiment, the skin moisture increase effect test was performed as described below, and at the same time, the questionnaires for the improvement evaluation of the skin dryness were made to the test subjects.

3-1. Skin moisture  Increase effect

Fifty women aged 20 to 40 years who had poor skin were selected from the cosmetic preparations prepared in the following formulation examples and comparative examples, and were given Formulation Example 1 and Comparative Formulation Example 1, respectively, and applied to the face and the first half for one month twice a day . At this time, the skin dryness was measured by using a moisture meter (CM820 courage Khazaka electronic GmbH, Germany) in a constant temperature and humidity condition (24 ° C, 40% , 2 weeks, and 5 weeks after the treatment. At this time, as the negative control group, the group not containing the extract was used, and as the positive control group, the lipid mixture used in Experimental Example 2 was used.

3-1-2. Formulation Example ( Emulsifier type )

In the compositions shown in Table 3 below, the oil and water phases were completely dissolved at 70 DEG C and emulsified at 7,000 rpm for 5 minutes to prepare a cream formulation.

[Table 3]

Component (% by weight) Formulation Example 1 Comparative Formulation Example 1 Comparative Formulation Example 2 Beads wax 2 2 2 Stearyl alcohol 5 5 5 Stearic acid 8 8 8 Squalene 10 10 10 Propylene glycol monostearate 3 3 3 Polyoxyethylene ether One One One Preservative, antioxidant dose dose dose glycerin 4 4 4 Propylene glycol 8 8 8 Triethylamine One One One Sodium diol polyacrylate 0.2 0.2 0.2 Purified water Up to 100 Up to 100 Up to 100 Example 1 (fermented extract of herbal medicines) 2 - - Comparative Example 1 (herbal medicine extract) - 2 - Lipid mixture - - 2

3-1-3. result

As shown in Table 4, skin moisture was increased in Formulation Example 1 containing the fermented extract of herbal medicines.

[Table 4]

division Skin moisture increase rate (%) 1 week Two weeks Three weeks Formulation Example 1 59 65 78 Comparative Formulation Example 1 31 40 46 Comparative Formulation Example 2 42 53 61

3-2. Improved skin dryness  Evaluation (Survey)

As shown in the following Table 5, the subjective evaluation of the dryness of the subjects was evaluated, and the results are shown in Table 5. [Table 5] < EMI ID = , Formulation Example 1 containing the fermented extract of herbal medicines obtained excellent results.

[Table 5]

division Number of Respondents Very good Good usually Inadequate Formulation Example 1 33 15 2 0 Comparative Formulation 1 5 12 18 15 Comparative Formulation Example 2 5 11 23 11

Claims (5)

Ginseng, peony, clove, ginseng, ginseng, ginseng, safflower, pomegranate, ginseng, ginseng, ginseng, ginseng, A cosmetic composition for skin moisturizing, characterized by containing a fermented extract of a mixture of herbal medicines composed of dermis, creation, vulgaris softening, chrysanthemum, persimmons, passerine and red ginseng. The cosmetic composition for skin moisturizing according to claim 1, wherein the fermented extract of the herbal medicine mixture is contained in an amount of 0.0001 to 10% by weight based on the total weight of the composition. The method according to claim 1, wherein the fermentation is performed using at least one yeast selected from the group consisting of Saccharomyces sp ., Schizosaccharomyces sp ., Torulopsis sp ., Rhodotorula sp ., And Candida sp . Cosmetic composition. The cosmetic composition for skin moisturizing according to claim 1, wherein the fermentation is performed using at least one lactic acid bacterium selected from the group consisting of Lactobacillus sp ., Streptococcus sp ., Leuconostoc sp ., And Bifidobacteria sp . The cosmetic composition for skin moisturizing according to claim 1, wherein the cosmetic composition is a formulation of an external ointment for skin, a softening agent, a nutritional lotion, a nutritional cream, a massage cream, an essence, a pack, an emulsion or an oil gel.