KR101456056B1 - Anti-wrinkle cosmetic composition - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving wrinkles, and more particularly to a cosmetic composition for improving wrinkles containing a fermented extract of red ginseng as an active ingredient. The cosmetic composition for wrinkle improvement according to the present invention is excellent in skin-safe and collagen synthesis promoting effect, and is excellent in skin wrinkle improving effect, wound healing effect and persistence of effect.

Description

[0001] Anti-wrinkle cosmetic composition [

The present invention relates to a cosmetic composition for improving wrinkles, and more particularly, to a cosmetic composition for improving wrinkles, which is excellent in skin promoting effect and promotes collagen synthesis, and has excellent skin wrinkle improving effect, wound healing effect and sustained effect .

Collagen, a major component of the extracellular matrix, is a major substrate protein in the fibroblasts of the skin and is present in extracellular epilepsy. It is also an important protein that accounts for about 30% of the total weight of bioproteins, and has a solid triple helix structure. Collagen forms most of the organic matter in the skin, tendons, bones and teeth, especially in bone and skin (jaw). In most other structures, it exists as a fibrous inclusion.

Collagen is a relatively weak immunogen, partly due to the blocking of the latent antigenic determinant by the helical structure of the collagen, which also causes the collagen to be resistant to proteolysis. The main functions of collagen are known to be mechanical rigidity of the skin, joint strength of the connective tissues and binding force of tissues, support of cell adhesion, induction of cell division and differentiation (when organism grows or heals the wound) (Van der Rest et al. , Ann NY Acad Sci, 1990).

Such collagen is reduced by aging and photoaging by ultraviolet irradiation, which is known to be closely related to the wrinkling of the skin (Arthur K. Balin et al., Aging and the skin, 1989). Collagen also plays an important role in wound healing and can accelerate the synthesis of collagen in the damaged epithelium, allowing quick, scarless wound healing.

Conventionally, products using collagen in a composition for external application for skin such as cosmetics or ointment have been marketed in order to utilize the skin moisturizing effect and wound healing effect of collagen. However, these products apply collagen itself to the skin surface, It is impossible to expect a moisturizing action or a wound healing effect, so that it can not be said that it exhibits essential skin function improvement and wound healing function.

In order to solve this problem, there has been a growing interest in collagen synthesis promoting substances. Vitamin C, retinoic acid, transforming growth factor (TGF) (Cardinale G. et al. (Japanese Patent Application Laid-open No. Hei 8-231370), betulinic acid (Japanese Patent Application Laid-Open No. 8-208424) Chlorella extract (JP-A-9-40526, JP-A-10-36283, fibroblast proliferation promoting action), and the like.

However, these materials have limited use due to safety problems such as irritation and reddening when applied to the skin, or the effect is insignificant and virtually no effect of skin function improvement or wound healing can be expected. Therefore, it is urgently required to develop a novel composition for external skin application which is safer and more effective in living body than existing skin external composition compositions.

On the other hand, fermentation is a process of decomposing organic matter using enzymes possessed by microorganisms. Fermentation and corruption proceed by a similar process, but decomposition is called fermentation when substances that are useful for our lives are made, and when they make odorous or harmful substances, it is called corruption. Therefore, if the herb medicine is fermented using microorganisms, the toxicity of the herb medicine is reduced, the low molecular weight is easily digested, and the transdermal absorption upon application to the skin becomes easy, and the bioavailability is improved.

The herbal medicines themselves can exhibit pharmacological effects, but the pharmacological effects can be further strengthened by fermentation or harmful components can be produced by the metabolism of microorganisms, so that a new pharmacological effect can be exhibited which is not present in the herbal medicine before fermentation.

In order to solve the problems of the prior art as described above, it is an object of the present invention to provide a cosmetic composition for improving wrinkles, which is excellent in skin-promoting effect and collagen synthesis promoting effect and is excellent in skin wrinkle improving effect, wound healing effect, .

Another object of the present invention is to provide a cosmetic composition for improving wrinkles in which the pharmacological effect of each medicinal substance is further enhanced through fermentation, thereby further enhancing collagen synthesis promoting effect.

In order to attain the above object, the present invention provides a method for producing a pine nut, comprising the steps of: preparing a pine nut, a pine nut, a pine nut, a pine nut, a pine nut, Characterized in that it comprises as an active ingredient a fermented extract of a medicinal substance selected from the group consisting of persimmon, ginseng, peony, clove, sculptor, dermis, creation, The present invention provides a cosmetic composition.

The fermentation extract can be obtained by adding a medicinal substance to yeast, lactic acid bacteria or bifidobacteria and culturing the same.

The fermented extract can be obtained by inoculating fungi into a medicinal material and solid fermentation.

The medicinal fermentation extract is preferably contained in the cosmetic composition in an amount of 0.0001 to 10% by weight.

The cosmetic composition may be formulated into ointment for external use, softening longevity, nutritional lotion, nutritional cream, massage cream, essence, pack, emulsion, oil gel and the like.

The cosmetic composition for wrinkle improvement according to the present invention enhances the pharmacological effect of each medicinal substance through fermentation while safely protecting the skin, thereby effectively promoting collagen synthesis, and thus has excellent skin wrinkle improving effect, wound healing effect and persistence of effect thereof.

Hereinafter, the cosmetic composition for wrinkle improvement of the present invention will be described in detail.

The medicinal plant extracts described in the present invention can be used as a medicinal herb extract or a medicinal herb extract of the present invention as a medicinal herb extract of Lactobacillus japonica L. var. Refers to an extract obtained by extracting one or more kinds of medicinal materials selected from ginseng, peony, clove, sculptor, dermis, creation, silkworm, silkworm, persimmon, and red ginseng according to a conventional method. Also, the medicinal fermentation extract means a secondary extract obtained by inoculating and culturing the above-mentioned medicinal plant extract into yeast, lactic acid bacteria or bifidus microorganisms, or obtained by solid fermentation by adding fungi to commercially available medicinal products or pulverized products.

The present inventors have conducted research to develop new collagen synthesis promoting substances for copper and plants fermented by useful microorganisms in order to develop a substance having promoting effect on collagen synthesis at a cellular level using human-derived fibroblasts. As a result, , Gobon, Kwakyanghyang, Gyungyung, Antler, Angelica, Jujube, Cordyceps, Myeonbyeol, Baekbokryeong, Baekji, Sine, Saengsheon, Pomegranate, Seokchangpo, Truffle mushroom, Songyu, Yeongji, Ohjeon, Yongdam, Ginseng, Peony, Clove, Sculptor, Dermis , And the fermentation broths such as Chinese herbaceous plants, Chinese herbaceous plants, silkworms, guinea pigs, goats, and red ginseng have remarkably superior collagen synthesis promoting effects than the respective extracts.

The cosmetic composition for wrinkle improvement according to the present invention is characterized in that the cosmetic composition for improving wrinkles is selected from the group consisting of licorice, gobon, kwakyang, gyeonggyeong, antler, angelica, jujube, cordyceps, And a fermented extract of a medicinal substance selected from the group consisting of ginseng, peony, clove, sculptor, dermis, creation, silkworm, silkworm, guinea pig, passerine and red ginseng.

The licorice (Glycyrrhiza glabra) is a perennial herb belonging to Legumisosae. It is used in medicinal or tea by drying in the shade. It is used for neuralgia, cough, arteriosclerosis, dysuria. Angelica tenuissima is a perennial plant belonging to Umbelliferae and has efficacy for abdominal pain, toothache and diarrhea. Agastache rugosa is a perennial herb that belongs to the genus Labiatae and is used in cases of gastritis, sweating, fever, and dryness. Platycodon gradiflorum is a perennial herb that belongs to the Campanulaceae and is used for cannibalism and toothache. The Cervus nippon is a dried plucked grass of a plum or locust belonging to the deer (Cervidae) and dried to protect the liver and the ginseng and strengthen the bones and muscles. The Angelica gigas are perennial roots belonging to the Umbelliferae and are used for body weakness, headache and constipation. The jujube (Zizyphus jujube) is a fruit of broad-leaved arboreous tree in Rhamnaceae and is used for nursing, tanning, detoxification, shinhae, and labor. The Cordyceps sinensis belongs to Hypocreaceae and is used for anticancer, arteriosclerosis, hypertension and heart disease. Aucklandia lappa is the root of perennial herbaceous plants belonging to the compositae. The Poria cocos belong to the Polypooraceae and are used for immobility, noise and diarrhea. The white paper (Angelica dahurica) is a biennial herb belonging to the Umbelliferae. It is used for headache, dizziness, Guan Wasa. Amomum villosum is a perennial herb that belongs to the genus Zingiberaceae and is used for vomiting, diarrhea, and early anxiety. Thymus vulgalis is a perennial plant belonging to the family Lamiaceae and is used for headache, depression, anemia, and fatigue. The pomegranate (Punica granatum) is a perennial deciduous broad-leaved arboreous tree belonging to the pomegranate family (Punicaceae) and is used for arteriosclerosis, diarrhea, athlete's foot, and the like. The above-mentioned Acorus tatarinowii belongs to the genus Araceae and is used for forgetfulness, purulent tumor, bruise. The tuber melanosporum is an underground raw mushroom of a Western tongue, and is used for edible purposes. Tricholoma matsutake is a mushroom of bacillus subtilis. It is used for the suppression of cholesterol in blood, the promotion of blood circulation (hand, foot trembling, treatment of back and knee palsy), arteriosclerosis, heart disease, diabetes and hyperlipemia. Ganoderma lucidum is a fruiting body of Ganoderma Lucidum and its family members belonging to Polypooraceae and is used for arteriosclerosis, neuralgia, cancer and the like. Lindera strichnifolia is a root of Tiantai mushroom belonging to the genus Lauraceae, and has the efficacy of the bladder cold, the urine odor, the opacity, and the mid-storm. Gentiana scabra is a perennial herb that belongs to Gentianaceae and is used for the production of swine, epilepsy, roundworms, and eczema. Ginseng is a perennial herbaceous perennial belonging to the genus Araliaceae. It is used for blood circulation, cold circulation and bronchitis. Paeonia albiflora is a perennial herb belonging to Ranunculaceae and is used for accelerating blood circulation, analgesia and asthma. The clove (Eugenia caryophyllata) is a dried flower of a celery belonging to an evergreen tree belonging to the genus Myrtaceae. It is used for the treatment of facial, mouth odor, and sexual function. The sculptor (Gleditsia sinensis) is dried in the visible part of the sculptured tree belonging to the Reguminosae, and is used for boils and insecticides. The dermis (Citruunshiu) is a dry mandarin ripened fruit shell belonging to the Ruraceae. The creation (Atractylodes chinensis) is a perennial herb that belongs to the compositae and is dried in the trunks of the buds, and is used for strokes, conjunctivitis, hypertension, and dizziness. The Saussurea involucrate is a perennial herb that belongs to Ranunculaceae and is used as an analgesic, a sorghum, a cardiotonic agent, a diuretic, and the like. Zanthoxylum piperitum is a mature periwinkle of the Sancho wood belonging to the Ruraceae, and is used for abdominal pain, diarrhea, antiparasitic action, and skin eczema. The passerine (Prunus armeniaca) is a mature fruit seed of an apricot tree belonging to the Rosaceae family, and is used for the treatment of coughing and breathing. Red gienseng is steamed steamed ginseng, which is used to treat arteriosclerosis, diabetes, restoration, and sexual function.

The medicinal materials may be prepared by a conventional method, for example, by extracting with an organic solvent such as distilled water, anhydrous or low-boiling alcohol having 1 to 4 carbon atoms, concentrating under reduced pressure and then drying to obtain a first extract . Specifically, there are commercially available ginseng products such as licorice, gobon, kwakyang, gyeonggyeong, antler, angelica, jujube, cordyceps, myeonbyeol, Baekbokryeong, Baekji, sine, safflower, pomegranate, , 5 to 20 parts by volume of water based on the dry weight of the pulverized material, 1 to 4 carbon atoms or anhydrous or hydrocarbons of 1 to 4 carbon atoms, The extracts of the medicinal materials are extracted by heating in an extractor equipped with a reflux condenser with an organic solvent such as a functional lower alcohol for 1 to 5 hours at 50 to 100 ° C. After filtration with a filter cloth, the residue is further extracted one or more times by the same method. The extracts are combined, concentrated under reduced pressure, and lyophilized or spray dried to obtain a dry extract.

The herbal extracts obtained as described above may be added to yeast, lactic acid bacteria or bifidus bacteria and cultured to obtain a final fermented extract. At this time, it is preferable that the medicinal plant extract is added to the basic medium of yeast, lactic acid bacteria or bifidobacteria in an amount of 1 to 20% by weight. In addition, the pharmaceutical fermentation extract may be obtained by inoculating the commercially available medicinal product itself or a slice or powder into a fungi, followed by solid fermentation.

The yeast may be Saccharomyces cerevisiae , Schizosaccharomyces ellipsoids , Saccharomyces boulardii or the like.

The lactic acid bacteria may be selected from the group consisting of Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus confusus, Lactobacillus fermentum and Lactobacillus brevis .

Examples of the bifidobacteria include Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum , Bifidobacterium infantis , and the like.

The fungus can be a medicinal mushroom mold such as Inonnotus-obliquus, Cordyceps sinensis, Paecilomyces japonica , Tricholoma matsutake, phellinus linteus and Ganoderma lucidum. have.

More specifically, when yeast is used as a microorganism, it has a constant concentration (about 10 ⁵ to 10 ⁷ cells / mL) , First extract of red ginseng, persimmon, persimmon, ginseng, red ginseng, red ginseng, pine ginseng, seokchonpo, truffle mushroom, mulberry, ganoderma, omega, yongdam, ginseng, peony, clove, Yeast is inoculated in the primary culture medium (YM medium) and cultured for 30 to 120 hours under conditions of pH 5.5 to 6.5, temperature 25 to 35 ° C, aeration amount 0.05 to 0.5 VVM. Thereafter, the fermentation broth is centrifuged to remove the polymer precipitate and the yeast body, and the concentrate is concentrated under reduced pressure at 60 ° C in a distillation apparatus equipped with a cooling condenser, followed by lyophilization, thereby obtaining each of the fermentation extracts of medicinal plants.

When lactobacillus is used as a microorganism, it has a constant concentration (about 10 ⁵ to 10 ⁷ cells / mL), and there is a low concentration of liquorice, gobon, (MRS medium) containing the first extracts of red ginseng, red ginseng, red ginseng, red ginseng, red ginseng, tsushinpu, truffle, tsuyu, , And cultured for 24 to 120 hours under the conditions of pH 6.5 to 7.5, temperature 35 to 38 占 폚, and aeration amount of 1 VVM. Thereafter, the fermentation broth is centrifuged to remove the polymer precipitate and the lactic acid bacterium, and the concentrate is concentrated under reduced pressure at 60 ° C in a distilling apparatus equipped with a cooling condenser, followed by lyophilization, thereby obtaining each of the fermentation extracts of medicinal plants.

When bifidobacteria are used as microorganisms, there is a certain concentration (about 10 ⁵ to 10 ⁷ cells / mL) of licorice, gobon, kwakyeong, gakyung, antler, angelica, jujube, (BL medium) containing primary extracts of Korean red ginseng root, Seokchangpo, Truffle mushroom, Songyi, Youngji, Ohjang, Yongdam, Ginseng, Peony, Clove, Sculptor, Dermis, Creation, ), Followed by culturing for 24 to 120 hours at pH 6.5 to 7.5 at a temperature of 35 to 38 DEG C while maintaining the anaerobic culture condition. Thereafter, the fermentation broth is centrifuged to remove the polymer precipitate and bifidus microbial cells, and the suspension is concentrated in a distillation apparatus equipped with a cooling condenser at 60 ° C and freeze-dried to obtain the respective fermentation extracts of medicinal products.

In addition, when fungi is used as a microorganism, fungi such as licorice, gobon, kwakyang, gyeonggyeong, antler, angelica, jujube, Fungus is inoculated in an amount of 1 to 10% by weight in each of the crushed, crushed, salted, ginseng, peony, clove, sculptor, dermis, And fermented for 60 days at ~ 25 ° C and 60-70% humidity to obtain each of the medicinal fermentation extracts.

The above medicinal fermentation extracts exhibit remarkably superior promoting effect on collagen synthesis when they are treated with human fibroblasts as compared with general medicinal plant extracts. In addition, when the cosmetic composition is manufactured using the above fermented extracts of medicinal materials, it is safe to the skin without any side effects when applied to human skin, and exhibits excellent skin wrinkles and wound healing effects.

The medicinal fermentation extract obtained as described above is preferably contained in the cosmetic composition for improving wrinkles of the present invention in an amount of 0.0001 to 10% by weight, more preferably 0.0005 to 10% by weight. When the content is less than 0.0001% by weight, no remarkable effect can be expected. When the content is more than 10% by weight, there is no evidence of a marked effect due to the increase of the content.

The formulation of the cosmetic composition for improving wrinkles containing the above fermented extract of the present invention as an active ingredient is not particularly limited and may be appropriately selected according to the purpose. For example, the cosmetic composition of the present invention may be formulated into ointment for external use, softening longevity, nutritional lotion, nutritional cream, massage cream, essence, pack, emulsion, oil gel and the like. In this case, the ointment for external use on skin may be prepared so as to contain 50 to 97% by weight of vaseline and 0.1 to 5% by weight of polyoxyethylene oleyl-ether phosphate in addition to the medicinal fermentation extract of the present invention. 1 to 10% by weight of polyhydric alcohols (propylene glycol, glycerin, etc.) and 0.05 to 2% by weight of a surfactant (polyethylene oleyl ether, polyoxyethylene hardened castor oil, etc.). The nutritional lotion and nutritional cream may contain 5 to 20% by weight of oils (squalane, petrolatum, octyldodecanol, etc.) and 3 to 15% by weight of wax components (cetanol, stearyl alcohol, %, And the essence can be prepared by adding 5 to 30% by weight of polyhydric alcohols such as glycerin, propylene glycol and the like in addition to the fermented extract of the present invention. The massage cream is prepared by adding 30 to 70% by weight of an oil such as liquid paraffin, petrolatum, isononylisononanoate and the like in addition to the medicinal fermented extract of the present invention. In addition to the fermented extract of the present invention, the pack contains polyvinyl alcohol A peel off pack or a general emulsifiable cosmetic composition containing a weight% of a pharmacologically active ingredient of the present invention is characterized in that the pharmaceutical composition of the present invention contains a 5 to 30 wt% pigment such as kaolin, talc, zinc oxide, titanium dioxide, ) Pack.

In addition, the cosmetic composition for wrinkle improvement of the present invention may contain, in addition to the above-mentioned ingredients, the ingredients such as oil, water, a surfactant, a moisturizer, a lower alcohol, a thickener, a chelating agent, a dye, a preservative, Can be appropriately mixed and used as needed.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following examples.

[Example]

Example 1

Ginseng, peony, clove, ginseng, ginseng, ginseng, safflower, pomegranate, ginseng, ginseng, ginseng, ginseng, 200 g of each ground product obtained by purchasing and crushing the dermis, creation, lignocellulosic, chrysanthemum, persimmon, passenger, and red ginseng were added to 3 L of purified water or 80% methanol, and the mixture was boiled for 3 hours in a reflux extractor equipped with a cooling condenser Respectively. After filtration through a 100-mesh filter cloth, the residue was extracted one more time by the same method. Each of the extracts was combined and filtered through a filter paper of Whatman No. 2 at room temperature to remove insoluble matters. The extract was concentrated under reduced pressure at 60 ° C in a distillation apparatus equipped with a cooling condenser, followed by lyophilization, ≪ / RTI > Table 1 below shows the dry weight of the extract per 100 g of medicament according to the extraction solvent.

Medicinal 80% methanol Purified water licorice 8.3 g 7.9 g Gobon 8.5 g 8.1 g Wow 6.3 g 5.8 g Gakyung 5.3 g 5.1 g velvet 6.6 g 5.4 g Angelica 9.2 g 8.3 g Jujube 10.2 g 9.3 g Cordyceps 6.6 g 6.1 g elecampane 6.8 g 6.2 g Baekbokryung 8.5 g 7.8 g blank 8.3 g 8.1 g sign 7.5 g 7.1 g Four Soba 4.3 g 4.1 g Pomegranate 6.2 g 5.6 g Seokchangpo 8.3 g 7.3 g Truffle 9.2 g 7.9 g cluster 7.4 g 6.8 g wisdom 8.1 g 7.1 g Broken egg 7.3 g 7.0 g Yongdam 11.5 g 10.1 g Ginseng 6.4 g 6.0 g Peony 7.3 g 6.0 g cloves 5.2 g 4.8 g Sculptor 7.5 g 7.3 g dermis 5.6 g 5.3 g Creation 7.6 g 6.5 g Cheon San 4.8 g 4.3 g Light flux 7.3 g 6.8 g Predominates 8.3 g 8.1 g Passerine 6.5 g 6.1 g Red ginseng 6.3 g 5.9 g

Examples 2 to 32

Each of the herbal extracts obtained using 80% methanol was added to 5% by weight of YM medium as a basic medium for Saccharomyces cerevisiae, which was a yeast, and fermented.

Specifically, Saccharomyces cerevisiae was inoculated into a yeast basic medium (YM medium) containing 5 wt% of each herbal extract at a concentration of about 10 ⁵ to 10 ⁷ cells / mL, Lt; 0 > C, and aeration amount of 0.05-0.5 VVM. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and the yeast organism, and then concentrated under reduced pressure at 60 DEG C in a distilling apparatus equipped with a cooling condenser, followed by freeze-drying to obtain a final fermented yeast fermentation product. The dry weight of the dried fermented product of the medicinal yeast is shown in Table 2 below.

Medicinal Dry weight of fermentation broth (g) Medicinal Dry weight of fermentation broth (g) Example 2 (licorice) 5.71 Example 18 (Songyi) 6.22 Example 3 (Giant) 6.11 Example 19 (manure) 6.18 Example 4 (outline) 6.23 Example 20 (off-white) 6.32 Example 5 (Gakyung) 6.00 Example 21 (Yongdam) 6.12 Example 6 (for antler) 6.12 Example 22 (Ginseng) 6.15 Example 7 (Angelica gigas) 5.97 Example 23 (Peony) 6.13 Example 8 (jujube) 6.11 Example 24 (Cloze) 6.02 Example 9 (Cordyceps) 6.16 Example 25 (Sculptor) 5.93 Example 10 (vinegar) 6.05 Example 26 (Dermis) 5.93 Example 11 (White rosewood) 6.10 Example 27 (Creation) 6.00 Example 12 (white paper) 6.07 Example 28 (Tetrachlorosilane synthesis) 6.11 Example 13 (Sign) 6.08 Example 29 (Chrysanthemum) 6.22 Example 14 (Sargassum) 6.10 Example 30 (Tridentate) 6.15 Example 15 (Pomegranate) 6.12 Example 31 (action) 5.93 Example 16 (Seok Changpo) 6.04 Example 32 (red ginseng) 6.04 Example 17 (Truffle) 6.05

Examples 33 to 63

Each of the herbal extracts obtained by using 80% methanol was added to 5% by weight of MRS medium as a basic medium for Lactobacillus acidophilus, and fermented.

Specifically, Lactobacillus acidophilus was inoculated into a lactic acid bacterial basic medium (MRS medium) containing 5 wt% of each of the herbal extracts at a concentration of about 10 ⁵ to 10 ⁷ cells / mL, and then pH 6.5 to 7.5 and temperature 35 to 38 , And a ventilation amount of 1 VVM was maintained for 48 hours. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and the lactic acid bacteria, followed by concentration under reduced pressure at 60 DEG C in a distilling apparatus equipped with a cooling condenser, followed by lyophilization to obtain a final fermented product of lactic acid bacteria. The dry weight of the fermented product of the medicinal lactic acid bacteria is shown in Table 3 below.

Medicinal Dry weight of fermentation broth (g) Medicinal Dry weight of fermentation broth (g) Example 33 (licorice) 5.73 Example 49 (Songyi) 5.89 Example 34 (Giant) 6.02 Example 50 (manure) 6.06 Example 35 (outline) 6.16 Example 51 (off-white) 5.97 Example 36 (Gakyung) 5.92 Example 52 (Yongdam) 6.04 Example 37 (antler) 6.15 Example 53 (Ginseng) 6.09 Example 38 (Angelica gigas) 6.05 Example 54 (Peony) 6.22 Example 39 (Jujube) 6.05 Example 55 (Cloze) 5.91 Example 40 (Cordyceps) 6.11 Example 56 (Sculptor) 6.12 Example 41 (vinegar) 6.01 Example 57 (dermis) 6.08 Example 42 (White rosewood) 5.99 Example 58 (Creation) 6.04 Example 43 (white paper) 5.91 Example 59 (Tetrazolium tetrafluoroborate) 6.05 Example 44 (Sine) 5.93 Example 60 (Chrysanthemum) 6.09 Example 45 (Saponin) 6.15 Example 61 (caustic soda) 6.02 Example 46 (Pomegranate) 5.98 Example 62 (action) 6.04 Example 47 (Seok Changpo) 6.12 Example 63 (red ginseng) 6.19 Example 48 (truffle) 5.99

Examples 64 to 94

Each of the herbal extracts obtained by using 80% methanol was added to the BL medium as a basic medium for bifidobacter bifidobacterium longum at 5 wt% and fermented.

Specifically, Bifidobacterium longum was inoculated into a lactic acid bacterial basic medium (BL medium) containing 5% by weight of each of the herbal extracts at a concentration of about 10 ⁵ to 10 ⁷ cells / mL, and then pH 6.5-7.5, temperature 35-38 ℃, and anaerobic culture conditions. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and bifidus microbial cells. The microbial precipitate and bifidus microbial cells were removed by a distillation apparatus equipped with a cooling condenser, followed by concentration under reduced pressure at 60 DEG C and freeze-drying to obtain a final fermented bifidus microorganism bacterium. The dry weight of the dried fermented product of the medicinal bifidus bacterium is shown in Table 4 below.

Medicinal Dry weight of fermentation broth (g) Medicinal Dry weight of fermentation broth (g) Example 64 (licorice) 5.83 Example 80 (Songyi) 6.26 Example 65 (Giant) 6.13 Example 81 (manure) 6.01 Example 66 (outline) 6.04 Example 82 (off-white) 6.03 Example 67 (Gakyung) 6.15 Example 83 (Yongdam) 6.14 Example 68 (for antler) 6.02 Example 84 (Ginseng) 6.15 Example 69 (Angelica gigas) 6.11 Example 85 (Peony) 6.03 Example 70 (jujube) 6.22 Example 86 (Cloisonne) 5.99 Example 71 (Cordyceps) 6.13 Example 87 (Sculptor) 6.16 Example 72 (vinegar) 6.00 Example 88 (Dermis) 6.20 Example 73 (White rosewood) 5.91 Example 89 (Creation) 6.17 Example 74 (white paper) 6.13 Example 90 (Tetrazolium tetrafluoroborate) 6.13 Example 75 (Sine) 6.04 Example 91 (Chrysanthemum) 6.26 Example 76 (silica gel) 6.13 Example 92 (caustic soda) 6.13 Example 77 (Pomegranate) 6.02 Example 93 (by-pass) 6.19 Example 78 (Seocheonpo) 6.04 Example 94 (red ginseng) 6.11 Example 79 (Truffle) 6.04

Examples 32 to 41

The medicinal materials used in Table 1 were sectioned and added with 5% by weight of fungi for solid fermentation.

Specifically, 200 g of each of the medicinal materials was used as a slice, Paecilomyces japonica was inoculated at 5% by weight under constant humidity, and the mixture was fermented for 60 days at a temperature of 18 to 25 캜 and a humidity of 60 to 70% g was added to 3 L of 80% methanol, and the mixture was boiled for 3 hours in a reflux condenser equipped with a condenser. After filtration with a 100 mesh filter cloth, the residue was extracted one more time by the same method. Each of the extracts was combined and filtered through a filter paper of Whatman No. 2 at room temperature to remove insoluble matter. The extract was concentrated under reduced pressure at 60 ° C in a distillation apparatus equipped with a cooling condenser and then lyophilized to obtain a final fermented product of the medicinal fungus. The dry weight of the dried fermented product of the medicinal fungus is shown in Table 5 below.

Medicinal Dry weight of fermentation broth (g) Medicinal Dry weight of fermentation broth (g) Example 95 (licorice) 7.52 Example 111 (Songyi) 4.80 Example 96 (Giant) 7.83 Example 112 (manure) 6.77 Example 97 (outline) 5.87 Example 113 (off-white) 6.62 Example 98 (Gakyung) 4.92 Example 114 (Yongdam) 9.02 Example 99 (for antler) 5.32 Example 115 (Ginseng) 5.43 Example 100 (Angelica gigas) 8.02 Example 116 (Peony) 5.12 Example 101 (jujube) 8.86 Example 117 (Cloze) 4.11 Example 102 (Cordyceps) 5.81 Example 118 (Sculptor) 6.83 Example 103 (vinegar) 5.67 Example 119 (dermis) 4.70 Example 104 (White rosewood) 7.11 Example 120 (Creation) 5.81 Example 105 (white paper) 7.64 Example 121 (Tetrazolium tetrafluoroborate) 3.87 Example 106 (Sine) 6.64 Example 122 (Chrysanthemum) 6.23 Example 107 (Sargassum) 4.07 Example 123 (caustic soda) 7.63 Example 108 (Pomegranate) 5.03 Example 124 (pass) 5.56 Example 109 (Seok Changpo) 6.78 Example 125 (red ginseng) 5.61 Example 110 (truffle) 6.80

EXPERIMENTAL EXAMPLE 1: Promoting collagen synthesis

Each of the herbal extracts obtained by using 80% methanol in Example 1 and the herbal extracts of Examples 2 to 110 were added to a culture solution of human-derived fibroblasts to examine the effect of collagen synthesis at the cellular level. The synthesized collagen was quantitated using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immunoassay KIT).

The medicinal fermentation extracts of Examples 2 to 110 were added to a culture medium of human fibroblast cells (human fibroblast cells) at a final concentration of 0.05%, and cultured for 1 day. Then, the culture broth was taken and cultured with PICP EIA Kit The degree of collagen synthesis was measured at 450 nm using a spectrophotometer. For the comparison of the effects, the degree of collagen synthesis was measured in the same manner as in the case of the culture medium of the fibroblasts to which nothing had been added (control group) and the vitamin C added to the final concentration of 0.005%.

The amount of collagen production was measured by UV absorbance, and the rate of increase of collagen production was calculated as a ratio of the amount of collagen production relative to the control. The results are summarized in Tables 6 to 10 below.

sample Amount of collagen production Growth rate (%) Control group (no addition) 10118 ± 0.030 - YM medium (final concentration 0.005%) 1.202 ± 0.0032 107.51 MRS medium (final concentration 0.005%) 1.215 + 0.041 108.68 BL medium (final concentration 0.005%) 1.223 + 0.041 109.39 licorice
(Final concentration: 0.005%) The extract of Example 1 1.332 + 0.060 119.14 The yeast fermented extract of Example 2 1.628 + 0.061 145.62 The fermented extract of lactic acid bacteria of Example 33 1.654 + 0.048 147.94 The bifidobacterial fermentation extract of Example 64 1.687 + 0.063 150.89 The fungal fermentation extract of Example 95 1.762 + 0.065 157.60 Gobon
(Final concentration: 0.005%) The extract of Example 1 1.312 + 0.021 117.35 The fermented yeast extract of Example 3 1.612 + 0.092 144.19 The fermented extract of lactic acid bacteria of Example 34 1.543 + 0.072 138.01 The bifidobacterial fermentation extract of Example 65 1.599 + 0.081 143.02 The fungal fermentation extract of Example 96 1.634 0.077 146.15 Wow
(Final concentration: 0.005%) The extract of Example 1 1.235 + - 0.052 110.47 The fermented yeast extract of Example 4 1.534 + 0.098 137.21 The fermented extract of lactic acid bacteria of Example 35 1.611 + 0.030 144.10 The bifidobacterial fermentation extract of Example 66 1.523 + 0.082 136.23 The fungal fermentation extract of Example 97 1.674 + 0.046 149.73

sample Amount of collagen production Growth rate (%) Gakyung
(Final concentration: 0.005%) The extract of Example 1 1.356 + 0.048 121.29 The fermented yeast extract of Example 5 1.660 + - 0.058 147.48 The fermented extract of lactic acid bacteria of Example 36 1.624 ± 0.056 145.26 The bifidobacterial fermentation extract of Example 67 1.629 + 0.072 145.71 The fungal fermentation extract of Example 98 1.691 + 0.083 151.25 velvet
(Final concentration: 0.005%) The extract of Example 1 1.272 + 0.034 113.77 The yeast fermented extract of Example 6 1.557 + 0.069 139.27 The fermented extract of lactic acid bacteria of Example 37 1.502 + 0.068 134.35 The bifidobacterial fermentation extract of Example 68 1.673 + 0.034 149.64 The fungal fermentation extract of Example 99 1.772 + 0.071 158.50 Angelica
(Final concentration: 0.005%) The extract of Example 1 1.242 + 0.067 110.91 The yeast fermented extract of Example 7 1.575 + 0.067 140.88 The fermented extract of lactic acid bacteria of Example 38 1.536 ± 0.056 137.39 The bifidobacterial fermentation extract of Example 69 1.600 + - 0.056 143.11 The fungal fermentation extract of Example 100 1.563 + 0.062 139.98 Jujube
(Final concentration: 0.005%) The extract of Example 1 1.357 ± 0.056 121.38 The yeast fermented extract of Example 8 1.768 + 0.045 158.14 The fermented extract of lactic acid bacteria of Example 39 1.623 + - 0.056 145.17 The bifidobacterial fermentation extract of Example 70 1.656 + 0.066 148.12 The fungal fermentation extract of Example 101 1.699 + 0.074 151.97 Cordyceps
(Final concentration: 0.005%) The extract of Example 1 1.267 + 0.024 113.33 The yeast fermented extract of Example 9 1.652 + 0.076 147.58 The fermented extract of lactic acid bacteria of Example 40 1.584 + 0.050 141.68 The bifidobacterial fermentation extract of Example 71 1.641 + 0.039 146.78 The fungal fermentation extract of Example 102 1.777 ± 0.058 158.94 elecampane
(Final concentration: 0.005%) The extract of Example 1 1.299 + 0.034 116.19 The yeast fermented extract of Example 10 1.622 + 0.080 145.08 The fermented extract of lactic acid bacteria of Example 41 1.755 + 0.045 156.98 The bifidobacterial fermentation extract of Example 72 1.512 + 0.065 135.24 The fungal fermentation extract of Example 103 1.740 + 0.033 155.64 Baekbokryung
(Final concentration: 0.005%) The extract of Example 1 1.223 + 0.098 109.40 The fermented yeast extract of Example 11 1.595 + - 0.054 142.67 The fermented extract of lactic acid bacteria of Example 42 1.566 + 0.097 139.18 The bifidobacterial fermentation extract of Example 73 1.656 + 0.077 148.12 The fungal fermentation extract of Example 104 1.672 + 0.065 149.55 blank
(Final concentration: 0.005%) The extract of Example 1 1.345 + 0.078 120.30 The fermented yeast extract of Example 12 1.736 + 0.078 157.42 The fermented extract of lactic acid bacteria of Example 43 1.867 + 0.045 166.99 The bifidobacterial fermentation extract of Example 74 1.811 + 0.090 161.99 The fungal fermentation extract of Example 105 1.653 + 0.047 147.85 sign
(Final concentration: 0.005%) The extract of Example 1 1.324 + - 0.053 118.42 The fermented yeast extract of Example 13 1.744 + 0.055 155.99 The lactic acid bacteria fermented extract of Example 44 1.623 + - 0.056 145.17 The bifidobacterial fermentation extract of Example 75 1.624 + 0.064 145.26 The fungal fermentation extract of Example 106 1.598 + 0.070 142.93

sample Amount of collagen production Growth rate (%) Four Soba
(Final concentration: 0.005%) The extract of Example 1 1.412 ± 0.054 126.30 The fermented yeast extract of Example 14 1.767 + 0.033 158.05 The fermented extract of lactic acid bacteria of Example 45 1.762 ± 0.056 157.87 The bifidobacterial fermentation extract of Example 76 1.658 ± 0.077 148.30 The fungal fermentation extract of Example 107 1.866 + 0.045 166.91 Pomegranate
(Final concentration: 0.005%) The extract of Example 1 1.315 + 0.088 117.62 The yeast fermented extract of Example 15 1.752 + 0.099 156.71 The fermented extract of lactic acid bacteria of Example 46 1.655 + 0.077 148.03 The bifidobacterial fermentation extract of Example 77 1.687 + - 0.051 150.89 The fungal fermentation extract of Example 108 1.701 + 0.086 52.15 Seokchangpo
(Final concentration: 0.005%) The extract of Example 1 1.284 + 0.081 114.85 The yeast fermentation extract of Example 16 1.556 + 0.033 139.18 The fermented extract of lactic acid bacteria of Example 47 1.634 ± 0.056 146.15 The bifidobacterial fermentation extract of Example 78 1.589 ± 0.056 142.13 The fungal fermentation extract of Example 109 1.611 + - 0.056 144.10 Truffle
(Final concentration: 0.005%) The extract of Example 1 1.332 + 0.038 119.14 The yeast fermented extract of Example 17 1.655 + 0.066 148.03 The fermented extract of lactic acid bacteria of Example 48 1.756 + 0.088 157.07 The bifidobacterial fermentation extract of Example 79 1.702 + 0.065 152.24 The fungal fermentation extract of Example 110 1.658 ± 0.078 148.30 cluster
(Final concentration: 0.005%) The extract of Example 1 1.351 + 0.048 120.84 The yeast fermented extract of Example 18 1.702 + - 0.057 152.24 The fermented extract of lactic acid bacteria of Example 49 1.767 + 0.055 158.05 The bifidobacterial fermentation extract of Example 80 1.788 + 0.073 159.93 The fungal fermentation extract of Example 111 1.677 ± 0.055 150.00 wisdom
(Final concentration: 0.005%) The extract of Example 1 1.323 + 0.076 118.34 The yeast fermentation extract of Example 19 1.632 ± 0.056 145.97 The fermented extract of lactic acid bacteria of Example 50 1.600 + - 0.051 143.11 The bifidobacterial fermentation extract of Example 81 1.534 + 0.067 137.21 The fungal extract of Example 112 1.712 + 0.063 153.13 Broken egg
(Final concentration: 0.005%) The extract of Example 1 1.345 + 0.077 120.30 The yeast fermented extract of Example 20 1.699 + 0.032 146.81 The fermented extract of lactic acid bacteria of Example 51 1.633 + 0.039 146.81 The bifidobacterial fermentation extract of Example 82 1.775 + 0.076 158.77 The fungal fermentation extract of Example 113 1.711 + - 0.052 153.04 Yongdam
(Final concentration: 0.005%) The extract of Example 1 1.266 + 0.048 113.24 The fermented yeast extract of Example 21 1.655 + 0.087 148.12 The fermented extract of lactic acid bacteria of Example 52 1.613 + 0.092 144.28 The bifidobacterial fermentation extract of Example 83 1.587 + 0.045 141.95 The fungal fermentation extract of Example 114 1.564 + 0.060 139.89 Ginseng
(Final concentration: 0.005%) The extract of Example 1 1.345 + 0.045 120.30 The yeast fermented extract of Example 22 1.744 ± 0.056 155.99 The fermented extract of lactic acid bacteria of Example 53 1.652 + 0.076 147.76 The bifidobacterial fermentation extract of Example 84 1.828 + 0.067 168.16 The fungal fermentation extract of Example 115 1.678 ± 0.055 150.09

sample Amount of collagen production Growth rate (%) Peony
(Final concentration: 0.005%) The extract of Example 1 1.343 + 0.045 120.13 The fermented yeast extract of Example 23 1.656 + 0.081 148.12 The fermented extract of lactic acid bacteria of Example 54 1.612 + 0.070 144.19 The bifidobacterial fermentation extract of Example 85 1.711 + 0.042 153.04 The fungal fermentation extract of Example 116 1.563 + - 0.054 139.80 cloves
(Final concentration: 0.005%) The extract of Example 1 1.343 + 0.044 120.12 The yeast fermented extract of Example 24 1.655 ± 0.056 148.12 The fermented extract of lactic acid bacteria of Example 55 1.743 + 0.044 155.90 The bifidobacterial fermentation extract of Example 86 1.656 + 0.050 148.12 The fungal extract of Example 117 1.899 + 0.072 169.86 Sculptor
(Final concentration: 0.005%) The extract of Example 1 1.242 + 0.033 111.09 The fermented yeast extract of Example 25 1.610 + 0.023 144.01 The fermented extract of lactic acid bacteria of Example 56 1.755 + 0.045 156.98 The bifidobacterial fermentation extract of Example 87 1.692 ± 0.056 151.34 The fungal fermentation extract of Example 118 1.501 + 0.072 134.26 dermis
(Final concentration: 0.005%) The extract of Example 1 1.377 + 0.044 123.17 The yeast fermented extract of Example 26 1.711 + 0.023 153.04 The fermented extract of lactic acid bacteria of Example 57 1.632 + 0.079 145.97 The bifidobacterial fermentation extract of Example 88 1.877 + 0.072 167.89 The fungal fermentation extract of Example 119 1.603 + 0.073 143.38 Creation
(Final concentration: 0.005%) The extract of Example 1 1.260 + 0.055 112.70 The yeast fermented extract of Example 27 1.623 + 0.079 145.17 The fermented extract of lactic acid bacteria of Example 58 1.545 + 0.067 138.01 The bifidobacterial fermentation extract of Example 89 1.688 + 0.088 150.98 The fungal fermentation extract of Example 120 1.597 + - 0.053 142.84 Cheon San
(Final concentration: 0.005%) The extract of Example 1 1.322 + 0.080 118.25 The fermented yeast extract of Example 28 1.599 + 0.055 143.02 The fermented extract of lactic acid bacteria of Example 59 1.536 + 0.067 137.39 The bifidobacterial fermentation extract of Example 90 1.730 0.048 155.60 The fungal fermentation extract of Example 121 1.677 ± 0.067 150.00 Light flux
(Final concentration: 0.005%) The extract of Example 1 1.356 + 0.093 121.29 The yeast fermented extract of Example 29 1.644 + - 0.052 147.05 The fermented extract of lactic acid bacteria of Example 60 1.722 + 0.044 154.02 The bifidobacterial fermentation extract of Example 91 1.681 + 0.082 150.36 The fungal fermentation extract of Example 122 1.634 + 0.067 146.15 Predominates
(Final concentration: 0.005%) The extract of Example 1 1.233 + - 0.056 110.29 The fermented yeast extract of Example 30 1.675 + 0.033 149.82 The fermented extract of lactic acid bacteria of Example 61 1.588 ± 0.052 142.04 The bifidobacterial fermentation extract of Example 92 1.544 ± 0.056 138.10 The fungal fermentation extract of Example 123 1.602 ± 0.056 143.29 Passerine
(Final concentration: 0.005%) The extract of Example 1 1.354 + 0.071 121.11 The fermented yeast extract of Example 31 1.762 + 0.077 157.60 The lactic acid bacteria fermented extract of Example 62 1.556 ± 0.056 139.18 The bifidobacterial fermentation extract of Example 93 1.665 ± 0.056 148.93 The fungal fermentation extract of Example 124 1.766 + 0.073 157.96

sample Amount of collagen production Growth rate (%) Red ginseng
(Final concentration: 0.005%) The extract of Example 1 1.353 + 0.066 121.02 The fermented yeast extract of Example 32 1.755 ± 0.052 156.98 The fermented extract of lactic acid bacteria of Example 63 1.658 + 0.066 148.30 The bifidobacterial fermentation extract of Example 94 1.813 + - 0.052 162.16 The fungal fermentation extract of Example 125 1.772 + 0.064 158.50 Vitamin C (final concentration 0.0005%) 1.528 + 0.075 136.67

As shown in Tables 6 to 10, the fermentation extracts of each of the medicinal fermentation extracts obtained in Examples 2 to 125 were significantly increased in collagen production in human fibroblasts as compared with the extracts using 80% methanol in Example 1, Considering that it was not purified at all, it was found that fibroblasts had a strong ability to promote collagen synthesis as compared with vitamin C, which is a known promoter of collagen synthesis.

EXPERIMENTAL EXAMPLE 2. Promotion of Collagen Synthesis by Concentration

The human fibroblasts cultured in the same manner as in Experimental Example 1 were adjusted to 0.5%, 0.05%, 0.005%, and 0.0005%, respectively, of the licorice fungus fermentation extract of Example 95 to obtain collagen synthesis And the results are shown in Table 11. < tb > < TABLE >

sample Application concentration (%) Melanin production Inhibition rate (%) Control group (no addition) - 1.118 + 0.030 - The licorice fungus fermentation extract of Example 95 Final concentration (0.0005%) 1.502 + 0.043 134.35 Final concentration (0.005%) 1.762 + 0.065 157.60 Final concentration (0.05%) 1.888 + 0.078 168.87 Final concentration (0.5%) 1.923 + - 0.051 135.94 Vitamin C Final concentration (0.0001%) 1.528 + 0.075 172.00

As shown in Table 11, it was confirmed that the licorice fungus fermentation extract had a very strong collagen synthesis promoting activity against cultured human-derived fibroblasts. The extract of fermentation fungus fungus fermentation extract of the present invention has a capability of promoting collagen synthesis equal to or higher than that of vitamin C, which is a single substance, although it is not a single substance but a single substance and the extract of fermentation fungus fungus fermentation of the present invention shows no cytotoxicity even at a concentration of 0.05% It was predicted that it could be very useful as a skin wrinkle remover or a wound healing agent because it has strong collagen synthesis promoting ability.

Example  126 and Comparative Example  One

Using the fermentation extract of licorice fungus of Example 95, ointment for external skin was prepared as shown in Table 12 below.

division Example 126 Comparative Example 1 Licorice fungus fermentation extract 10 - Diethyl sebacate 8 8 Nonsense 5 5 Polyoxyethylene oleyl ether phosphate 6 6 Sodium benzoate Suitable amount Suitable amount vaseline To 100 To 100

Example 127 and Comparative Example 2

Using the fermented licorice fermented extract of Example 95, a cream was prepared according to the prescription shown in Table 13 below.

division Example 127 Comparative Example 2 Licorice fungus fermentation extract 1.0 - Stearic acid 15.0 15.0 Cetanol 1.0 1.0 Potassium hydroxide 0.7 0.7 glycerin 5.0 5.0 Propylene glycol 3.0 3.0 antiseptic Suitable amount Suitable amount incense Suitable amount Suitable amount Purified water To 100 To 100

Example 128 and Comparative Example 3

Using the fermented licorice fermented extract of Example 95, the softening longevity was prepared according to the formulation shown in Table 14 below.

division Example 128 Comparative Example 3 Licorice fungus fermentation extract 0.2 - ethanol 10.0 10.0 Polyoxyethylene sorbitan polylauric acid 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 glycerin 5.0 5.0 1,3-butylene glycol 6.0 6.0 incense Suitable amount Suitable amount Pigment Suitable amount Suitable amount Purified water To 100 To 100

Example 129 and Comparative Example 4

Using the fermented licorice fermented extract of Example 95, a nutritional lotion was prepared according to the formulation shown in Table 15 below.

division Example 129 Comparative Example 4 Licorice fungus fermentation extract 0.1 - Vaseline 2.0 2.0 Sesquioleic acid sorbitan 0.8 0.8 Polyoxyethylene oleylethyl 1.2 1.2 Methyl paraoxybenzoate Suitable amount Suitable amount Propylene glycol 5.0 5.0 ethanol 3.2 3.2 Carboxyvinyl polymer 18.0 18.0 Potassium hydroxide 0.1 0.1 Pigment Suitable amount Suitable amount incense Suitable amount Suitable amount Purified water To 100 To 100

Example 130 and Comparative Example 5

Using the fermented licorice fermented extract of Example 95, a pack was prepared according to the prescription of Table 16 below.

division Example 130 Comparative Example 5 Licorice fungus fermentation extract 0.5 - glycerin 5.0 5.0 Propylene glycol 4.0 4.0 Polyvinyl alcohol 15.0 15.0 ethanol 8.0 8.0 Polyoxyethylene oleylethyl 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 incense Suitable amount Suitable amount Pigment Suitable amount Suitable amount Purified water To 100 To 100

Example 131 and Comparative Example 6

Using the fermented licorice fermentation extract of Example 95, an essence was prepared according to the prescription of Table 17 below.

division Example 131 Comparative Example 6 Licorice fungus fermentation extract 5.0 - Propylene glycol 10.0 10.0 glycerin 10.0 10.0 Sodium hyruronate aqueous solution (1%) 5.0 5.0 ethanol 5.0 5.0 Polyoxyethylene hardened castor oil 1.0 1.0 Methyl paraoxybenzoate 0.1 0.1 incense Suitable amount Suitable amount Purified water To 100 To 100

Experimental Example 3. Effect of wound healing by collagen synthesis

Five - week - old male rats were tested for wound healing using a tensile strength method that reflects the quality and quantity of wound regeneration in incisions.

First, hair was removed from the back of the rat, incised with a scalpel and sutured. The creams of Example 127 and Comparative Example 2 were administered to the incision sites once a day for 6 days. Six days after the administration, the rats used in the experiment were slaughtered and the skin of the wound area was extracted. Three 1-cm pieces of skin having a width of 1 cm orthogonal to the wire line were prepared for each individual and rupture tension was measured with a rheometer. The measured tensile strength (g / cm) was taken as an index of the strength of regenerated collagen fiber. The increase rate of tensile strength was represented by the relative strength at 100% of the tensile strength of the control group to which ethanol alone was added without adding the sample. The strength measurement results are summarized in Table 18 below.

division Tensile strength (g / cm) Growth rate (%) Example 127 563 100 Comparative Example 2 659 117

Experimental Example 4. Effect of improving wrinkles

A panel test was conducted on the skin wrinkle reducing effect using the creams of Example 127 and Comparative Example 2.

First, 30 healthy women from 35 to 50 years old were divided into two groups by 15 persons, the cream of Comparative Example 2 was applied to Group 1, the cream of Example 127 was applied to the face portion for 3 months once a day Respectively. After 3 months, the improvement of skin wrinkles was evaluated by the questionnaire survey. As to the improvement of skin wrinkles and the elasticity of the subjects, the three stages of no improvement, slight improvement and significant improvement as compared with before use were determined, and the results are shown in Table 19 below.

division No improvement Slight improvement A significant improvement Example 127 One 7 7 Comparative Example 2 8 6 One

As shown in Table 19, when the cream of Example 127 according to the present invention was used, it was found that the effect of improving skin wrinkles and improving elasticity was excellent.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the invention as defined in the appended claims. It is obvious to those who have.

Claims (4)

A cosmetic composition for promoting collagen biosynthesis characterized by comprising a fermented extract of red ginseng as an active ingredient. The method according to claim 1,
The fermented extract may be selected from the group consisting of Saccharomyces cerevisiae , Schizosaccharomyces ellipsoids , Saccharomyces boulardii , Lactobacillus acidophilus, Lactobacillus acidophilus , Lactobacillus casei , Lactobacillus bulgaricus , Lactobacillus rhamnosus , Lactobacillus plantarum , Lactobacillus reuteri , Lactobacillus confusus, Lactobacillus spp . , Lactobacillus spp . , Lactobacillus spp. Lactobacillus fermentum , Lactobacillus brevis , Bifidobacterium breve , Bifidobacterium bifidum , Bifidobacterium longum, Bifidobacterium longum, Bifidobacterium longum, Long sword) and Bifidobacterium infantis (Bifidobacterium infantis Wherein the extract comprises fermented herbal extract obtained by adding and cultivating red ginseng when cultivating at least one bacterium selected from the group consisting of bacteriophage, The method according to claim 1,
The fermented extracts may contain at least one fungi selected from the group consisting of Inonnotus-obliquus, Cordyceps sinensis, Paecilomyces japonica, phellinus linteus and Ganoderma lucidum, And a fermentation extract obtained by solid fermentation, as an active ingredient. The method according to claim 1,
Wherein the fermentation extract is contained in the cosmetic composition in an amount of 0.0001 to 10% by weight.