KR20090002681A - Cosmetic composition having antioxidation activity - Google Patents

Abstract

A cosmetic composition having an antioxidizing activity, and a method for preparing the cosmetic material are provided to enhance the maintenance of antioxidizing effect and antiaging effect. A cosmetic composition having an antioxidizing activity contains the fermentation extract which is obtained by fermenting the mushroom fungi of at least one selected from Glycyrrhiza glabra, Angelica gigas, insam (ginseng), Scutellaria baicalensis and Poria cocos with yeast, lactobacillus and bifidus, and extracting it, as an active ingredient.

Description

Cosmetic composition having antioxidant activity {Cosmetic composition having antioxidation activity}

The present invention relates to a cosmetic composition having an antioxidant activity, and more particularly, to a cosmetic composition having an antioxidant activity that is safe for the skin and has excellent skin anti-aging and antioxidant effects and long-lasting effects thereof.

In general, there are many kinds of methods for explaining skin aging, and among them, the free radical theory that the skin is aged by free radicals caused by various causes in and out of the body is accepted as the most valid theory. Free radicals are chemically highly reactive chemicals, which are produced by various causes in the human body: external factors such as ultraviolet light, radiation, exhaust gas, tobacco smoke, stress, and metabolism of mitochondria and white blood cells in the body. In addition, the cascade leads to peroxidation of lipids, which are components of cell membranes.

Most of the free radicals associated with skin aging are Reactive Oxygen Species (ROS), which include super oxide radicals, hydrogen peroxide (H ₂ O ₂ ), and hydroxy radicals (Hydroxy radicals). ), And singlet oxygen. The peroxidation of lipids caused by them generates various reactive oxygen species of radicals, peroxides, aldehydes and epoxides and damages DNA, RNA, proteins, cell membranes and cellular structures.

These reactive oxygen species are thought to be the main cause of cancer, tissue damage, and aging (Black HS, Photochemphotobiol. 46 (2): 213 (1987)). For example, the peroxidation of the membrane lipids that make up the cell can change the structure and fluidity of the cell membrane, increase the permeability of the cytoplasm, leak the lysosomal enzyme, inactivate the membrane enzyme and the transport protein, create covalent crosslinks of lipids and proteins, and It causes chain breakage, DNA damage, mutations, and the like. Among these reactions, peroxidation of skin cell membranes and modification of dermal component proteins are known to be most closely associated with skin aging.

Aerobic organisms have protective mechanisms to prevent the formation of these oxidants and lipid peroxidation. These protective mechanisms include enzymes such as super oxide dismutase (SOD) and catalase, tocopherol (vitamin E), beta-carotene and ascorbic acid (ascorbic acid, vitamin C). And fat soluble and water soluble antioxidants or radical scavengers such as glutathione.

Meanwhile, skin anti-aging substances known to date have been developed in anticipation of the following effects. First, moisturizing the skin, second, antioxidant effect, third, promoting the proliferation of fibroblasts involved in the activation of skin cells, fourth, the enzyme that accelerates the formation of wrinkles and loss of elasticity by decomposing collagen and elastin constituting the skin Inhibiting the conversion of collagenase and elastase, and fifth, promoting collagen and elactin synthesis.

As described above, since free radical type oxygen or reactive oxygen species are associated with skin aging, conventionally, superoxide dismutase, vitamin E derivatives, vitamin C and derivatives thereof, flavonoids, and the like are applied to cosmetics. Although many efforts have been made to achieve the anti-aging effect by blending, it is difficult to expect substantial effects due to poor safety, stability, etc. when blending the above ingredients in cosmetics.

In addition, conventionally, antioxidants such as BHT (2,6-di-tertibutyl-4-methylphenol), BHA (2-tert0butyl-4-methoxyphenol) and PG (3,4,5-terhydroxy benzoic acid propyl ester) It was used a lot. However, there are drawbacks such as consumers' rejection of such synthetic antioxidants as the diet and living standards improve. In particular, BHT, a synthetic antioxidant most widely used in the past, has been reported to cause cancer when ingested at 50 mg / kg / day or more. Accordingly, studies to find a substance having antioxidant activity in natural materials such as heart have been carried out vigorously, but most of them had antioxidant capacity that did not meet expectations.

Therefore, in order to solve the problems of the prior art described above, there is a continuing need for development of products and raw materials with excellent active oxygen removal ability.

On the other hand, fermentation is a process of decomposing organic matter using enzymes possessed by microorganisms. Fermentation and decay are carried out by a similar process, but when decomposition produces a substance that is useful in our lives, it is called fermentation. Therefore, fermenting the herbal medicine using microorganisms reduces the toxicity of the herbal medicine, lowers the molecular weight, facilitates digestion, or facilitates transdermal absorption when applied to the skin, thereby improving bioavailability.

In addition, herbal medicines may exhibit pharmacological effects by themselves, but the pharmacological effects may be enhanced through fermentation, or harmful ingredients may be made by the metabolism of microorganisms, thereby exerting new pharmacological effects that were not found in herbal medicines before fermentation.

Therefore, the technical problem to be achieved by the present invention is to provide a cosmetic composition that is safe for the skin and has an antioxidant activity with excellent skin anti-aging and antioxidant effects and long-lasting effects.

Another object of the present invention is to further enhance the pharmacological effect of each medicine through fermentation to provide a cosmetic composition having an antioxidant activity of further improving collagen synthesis promoting effect.

In order to achieve the above technical problem, the present invention is a fermented extract extracted by fermenting mushroom fungi of lactic acid bacteria selected from the group consisting of licorice, Angelica, ginseng, gold, and Baekbongryeong with yeast, lactic acid bacteria or bifidus bacteria. It provides a cosmetic composition having an antioxidant activity, comprising as an active ingredient.

Hereinafter, the cosmetic composition having the antioxidant activity of the present invention will be described in more detail.

The cosmetic composition having the antioxidant activity of the present invention is effective for fermentation extract extracted by fermenting mushroom fermentation of mushrooms selected from the group consisting of licorice, Angelica, ginseng, golden, and Baekbongryeong with yeast, lactic acid bacteria or bifidus bacteria. It is characterized by including as a component.

The licorice (Glycyrrhiza glabra) is a perennial herb belonging to the legume (Legumisosae), dried in the shade and used as medicinal or tea, and used for neuralgia, cough, arteriosclerosis, and difficulty urination. Angelica gigas is a perennial root belonging to the genus Umbelliferae and is used for physical weakness, headache, constipation, and the like. The ginseng is a perennial root of the genus Ogari (Araliaceae) is used for blood circulation, cold hands and feet, bronchitis and the like. The golden (Scutellaria baicalensis) is a perennial herb of Lamiaceae, reaching about 1 m. The root is yellow so it is called golden. Total pravonoids are isolated and used for acute and chronic hepatitis and cholecystitis. Root alcohol extract is an antihypertensive and sedative agent used for hypertension, cardiovascular neuropathy, nervous system dysfunction, sleep disorders and palpitations. Inflammatory medicine and antipyretic medicine are used to remove the lungs and the colon. The Baekbokryeong (Poria cocos) belongs to the genus Polygonoraceae (Polypooraceae) and is used for gravitational force, sogal, diarrhea.

The medicinal herb can be prepared as an extract according to a conventional method. For example, the extract can be extracted with an organic solvent such as distilled water, anhydrous C1-C4 or hydrous lower alcohol, concentrated under reduced pressure, and dried to obtain a primary extract. have. Specifically, one or more medicinal herbs selected from the group consisting of licorice, Angelica, ginseng, gold, and Baeknyeongryeong, which are commercially available, are pulverized and pulverized, and 5 to 20 vol. An extract of the medicinal herbs is extracted by heating at 50-100 ° C. for 1-5 hours in an extractor equipped with a reflux condenser with an organic solvent such as 4 anhydrous or hydrous lower alcohols. After filtration with a filter cloth, the residue is further extracted one or more times in the same manner, the extracts are combined and concentrated under reduced pressure, lyophilized or spray-dried to obtain a dry extract.

Herbs selected from the group consisting of licorice, Angelica, ginseng, gold, and Baekbongryeong obtained as described above are first fermented through mushrooms. The fungus is a mushroom of medicinal mushrooms, such as chaga (Inonnotus-obliquus), Cordyceps sinensis, Paecilomyces japonica , Trichoma matsutake, phellinus linteus, Ganoderma lucidum, etc. Can be used.

Preferably, the mushroom bacteria fermentation is inoculated at 1 to 10% by weight of the mushroom bacteria in each of the medicinal herbs or slices, powdered ground powder selected from the group consisting of licorice, Angelica, ginseng, gold, and Baekbongnyeong After 60 days of solid fermentation at 18-25 ° C. and humidity of 60-70%, it can be extracted to obtain mushroom fermentation products.

Extraction of the mushroom fungi fermentation is 50 to 100 ℃ in an extractor equipped with a reflux cooler with an organic solvent, such as 5 to 20 volumes of water or an anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms based on the dry weight of the fermented product. Extract the extracts of the medicines by heating for ˜5 hours. Then, the filtrate was filtered with a filter cloth, and the residue was further extracted one or more times in the same manner, the extracts were combined under reduced pressure, concentrated under reduced pressure, and freeze-dried or spray-dried to obtain a dry extract.

The mushroom bacteria fermentation is then subjected to sterilization for smooth fermentation by yeast, lactic acid bacteria or bifidus bacteria. The mushroom bacteria fermentation may be added to each yeast, lactic acid bacteria or bifidus bacteria and cultured to extract the respective fermentation extracts. Preferably, the mushroom fermentation product is preferably added in an amount of 1 to 20% by weight to a medium for culturing yeast, lactic acid bacteria or bifidus bacteria.

The yeast may be used Saccharomyces cerevisiae , Schizosaccharomyces ellipsoids , Saccharomyces boulardii and the like.

Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus confusus, Lactobacillus fermentum, Lactobacillus brevis and the like can be used.

The bifidus bacteria may be Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum , Bifidobacterium infantis and the like.

Preferably, when the yeast is used as a microorganism, the yeast is inoculated into a basic medium (YM medium) each containing mushroom fungi fermentation of at least one selected from the group consisting of licorice, donkey, ginseng, gold, and Baekbongryeong ( After the desired concentration (about 10 ^{5-10 7} cells / mL), the cells are incubated for 30 to 120 hours while maintaining the conditions of pH ^5.5 to 6.5, temperature 25 to 35 ° C, and aeration amount of 0.05 to 0.5 VVM. The fermentation broth may be centrifuged to remove the polymer precipitate and the yeast cells, concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor, and then lyophilized to obtain respective medicinal fermentation extracts.

When using lactic acid bacteria as a microorganism, inoculate the lactic acid bacteria into a basic medium (MRS medium) containing fermented mushroom fungi of at least one kind selected from the group consisting of licorice, donkey, ginseng, gold, and Baekbongnyeong (preferred concentration ( About 10 ^{5-10 7} cells / mL), and incubate for 24 to 120 hours while maintaining the conditions of pH 6.5-7.5, temperature 35-38 ° C., and aeration rate 1 VVM. Removing the polymer precipitates and lactic acid bacteria, and concentrated under reduced pressure at 60 ℃ in a distillation apparatus with a cooling capacitor and then lyophilized to obtain the respective herbal fermentation extract.

When using bifidus as a microorganism, inoculate bifidus into a basic medium (BL medium) containing fermented mushroom fungi of at least one kind selected from the group consisting of licorice, donkey, ginseng, gold, and Baekbongryeong. After concentration (about 10 ^{5-10 7} cells / mL), the cells are incubated for 24 to 120 hours while maintaining pH 6.5-7.5, temperature 35-38 ° C., and anaerobic culture conditions. The precipitates and the bifidus cells were removed, concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor, and then lyophilized to obtain respective herbal fermentation extracts.

In addition, the total amount of the fermented extract of the medicinal herb in the cosmetic composition having an antioxidant activity of the present invention is preferably included in 0.0001 to 20% by weight, more preferably 0.01 to 10% by weight. If the content is less than 0.0001% by weight, no obvious effect can be expected.

The formulation of the cosmetic composition having the antioxidant activity of the present invention as described above is not particularly limited and may be appropriately selected as desired. For example, the cosmetic composition of the present invention may be prepared in the form of external skin ointment, softening longevity, nourishing longevity, nutrition cream, massage cream, essence, pack, emulsion, oil gel and the like. In this case, the skin external ointment may be prepared to contain 50 to 97% by weight of petrolatum and 0.1 to 5% by weight of polyoxyethylene oleyl-ether phosphate in addition to the medicinal fermentation extract of the present invention, the flexible cosmetics fermentation of the present invention In addition to the extract, it may be prepared to contain 1 to 10% by weight of polyhydric alcohols (propylene glycol, glycerin, etc.) and 0.05 to 2% by weight of surfactants (polyethylene oleyl ether, polyoxyethylene hydrogenated castor oil, etc.). In addition, nutrient cosmetics and nourishing cream is 5 to 20% by weight of oils (squalane, petrolatum, octyldodecanol, etc.) and wax components (cetanol, stearyl alcohol, beeswax, etc.) in addition to the medicinal fermentation extract of the present invention It can be prepared to contain%, essence can be prepared by containing 5 to 30% by weight of polyhydric alcohols such as glycerin, propylene glycol in addition to the medicinal fermentation extract of the present invention. Massage cream is prepared by containing 30 to 70% by weight of oil such as liquid paraffin, petrolatum, isononyl isononanoate in addition to the medicinal fermentation extract of the present invention, the pack is polyvinyl alcohol 5-20 in addition to the medicinal fermentation extract of the present invention Wash off containing 5-30% by weight of pigments such as kaolin, talc, zinc oxide and titanium dioxide in addition to the medicinal fermentation extract of the present invention in a peel-off pack or a general emulsion cosmetic containing ) Pack.

In addition, the cosmetic composition having the antioxidant activity of the present invention, in addition to the components described above in each formulation, oil, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, or fragrances that are formulated in general skin cosmetics Etc. can be mix | blended suitably as needed.

Cosmetic composition having the antioxidant activity of the present invention has a significantly superior antioxidant effect and persistence of the antioxidant effect compared to the case of using the yeast fermentation extract or lactic acid bacteria fermentation extract of the conventional medicine extract, and is harmless to the human body.

Hereinafter, preferred examples are provided to help understanding of the present invention, but the following examples are merely to illustrate the present invention, and the scope of the present invention is not limited to the following examples.

EXAMPLE

Example 1

Commercially available licorice, Angelica, Ginseng, Golden, and Baekbongnyeong, each of the pulverized 200g of each pulverized in purified water or 80% methanol 3L, boiled for 3 hours in a reflux extractor with a cooling capacitor and extracted. Then, the filtrate was filtered through a 100 mesh filter cloth and the residue was extracted once more in the same manner. Each extract was combined and filtered with Whatman No. 2 filter paper at room temperature to remove insoluble materials, concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor, and then freeze-dried to dry each dry weight as shown in Table 1 below. Got. Table 1 shows the dry weight of the extract per 100 g of medicinal herbs according to the extraction solvent.

Medicine 80% methanol Purified water licorice 8.3 g 7.9 g Donkey 6.5 g 6.1 g Ginseng 6.4 g 6.0 g Gold 6.8 g 6.1 g Baekbongryeong 8.5 g 7.8 g

The mushroom fermented product was solid fermented by adding 5% by weight of the mushroom bacteria to licorice, Angelica, ginseng, gold, and Baekbongnyeong obtained using 80% methanol.

Specifically, 200 g of Licorice, Angelica, Ginseng, Golden, and Baekbongnyeong were inoculated with 5% by weight of Paecilomyces japonica under constant humidity, and fermented and dried for 60 days at 18-25 ° C and 60-70% humidity. 100 g each of the pulverized product obtained by grinding was put into 3 L of 80% methanol, and the mixture was boiled and extracted with a reflux extractor equipped with a cooling capacitor for 3 hours. Then, the filtrate was filtered with 100 mesh filter cloth and the residue was extracted once more in the same manner. Each extract was combined and filtered with Whatman No. 2 filter paper at room temperature to remove insoluble matters, concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor, and lyophilized to obtain a final mushroom bacterial fermentation product. The dry weight of the medicinal mushroom mushroom fermented dry matter is shown in Table 2 below.

Medicine Dry Weight of Mushroom Fermentation (g) licorice 7.71 Donkey 7.78 Ginseng 7.81 Gold 7.62 Baikyeongryeong 7.65

The fermented fermented mushrooms were fermented by adding 5% by weight to YM medium, a basic medium for yeast Saccharomyces cerevisiae.

Specifically, Saccharomyces cerevisiae was inoculated in a yeast basal medium (YM medium) containing 5% by weight of mushroom fermentation at a concentration of about 10 ^{5-10 7} cells / mL, pH 5.5-6.5, temperature 25-35 C was incubated for 48 hours while maintaining the conditions of 0.05-0.5 VVM of airflow. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and the yeast cells, and then concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor, followed by lyophilization to obtain a final medicinal mushroom mushroom-yeast fermented dry matter. The dry weight of the medicinal mushroom bacteria-yeast fermented dry matter is shown in Table 3 below.

Medicine Dry Weight of Yeast Fermentation (g) licorice 5.71 Donkey 5.74 Ginseng 5.85 Gold 5.92 Baikyeongryeong 5.88

Example 2

Fermented mushroom fungus of Example 1 was added to MRS medium, which is a basic medium for Lactobacillus acidophilus, by 5 wt%.

Specifically, after inoculating Lactobacillus acidophilus to the lactic acid bacteria basic medium (MRS medium) containing the mushroom fermentation of Example 1 of 5% by weight at a concentration of about 10 ⁵ ~ 10 ⁷ cells / mL, pH 6.5 ~ 7.5 The mixture was incubated for 48 hours while maintaining the temperature at a temperature of 35 to 38 ° C and aeration rate 1 VVM. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and the lactic acid bacteria, and then concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor, followed by lyophilization to obtain a final medicinal herb mushroom-lactic acid fermentation dried product. The dry weight of the medicinal mushroom-lactic acid bacteria fermented dry matter is shown in Table 4 below.

Medicine Dry Weight of Lactic Acid Bacteria Fermentation (g) licorice 5.83 Donkey 5.84 Ginseng 5.95 Gold 5.82 Baikyeongryeong 5.84

Example 3

The fermented mushroom of Example 1 was added to the B medium medium Bifidobacterium longum Bifidobacterium longum in 5% by weight of fermentation.

Specifically, Bifidobacterium longum was inoculated in a lactic acid bacteria basic medium (BL medium) containing the mushroom fermentation of Example 1 of 5% by weight at a concentration of about 10 ⁵ ~ 10 ⁷ cells / mL, pH 6.5 ~ 7.5 The culture was carried out for 48 hours while maintaining the anaerobic conditions at a temperature of 35 to 38 ° C. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and the bifidus bacteria, and then concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor, followed by lyophilization to obtain a final medicinal herb mushroom-bifidus fermented dried product. . The dry weight of the medicinal mushroom bacteria-bifidus fermented dry matter is shown in Table 5 below.

Medicine Dry weight of bifidus fermentation (g) licorice 5.71 Donkey 5.74 Ginseng 5.85 Gold 5.92 Baikyeongryeong 5.88

Example 4-10

Licorice, Angelica, ginseng, golden, and Baeknyeong mushroom bacteria-yeast fermentation extracts, mushrooms-lactic acid bacteria fermentation extracts prepared in Examples 1 to 3, mushroom bacteria-bifidus fermentation extracts were mixed as shown in Table 6 below. Unit is weight ratio)

Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 Example 9 Example 10    licorice Fungi-Yeast 100 10 Mushrooms-Lactobacillus 100 50 20 10 Mushrooms-Bifidobacteria 100    Donkey Fungi-Yeast 10 Mushrooms-Lactobacillus 100 30 Mushrooms-Bifidobacteria 10    Ginseng Fungi-Yeast Mushrooms-Lactobacillus 10 Mushrooms-Bifidobacteria 100 20 10    Gold Fungi-Yeast 50 10 Mushrooms-Lactobacillus 100 20 10 Mushrooms-Bifidobacteria    Baikyeongryeong Fungi-Yeast 100 10 10 Mushrooms-Lactobacillus Mushrooms-Bifidobacteria 10

Experimental Example  One. Free radical  Erasure rate

Free radical scavenging activity was measured to confirm the antioxidant activity of Examples 1 to 10.

As the test sample, the yeast fermentation extract of licorice and lactic acid bacterium fermentation extract of ginseng were used, and the free radical scavenging activity was measured using DPPH. DPPH was purchased from Sigma Co., Ltd, USA. First, a standard DPPH solution of 400 μm concentration was made. In addition, ethanol was added to the yeast fermentation extract of licorice, lactic acid bacteria fermentation extract of ginseng, as an antioxidant ascorbic acid as Examples 1 to 10 and the reference material, respectively, to make a sample at a concentration of 200 ㎍ / ㎖. Then, the sample and the standard DPPH solution were added in the same ratio, stirred well, and then reacted at 37 ° C. for 30 minutes, and the absorbance was measured at 520 nm. At this time, ethanol was added instead of the above example as a control. Free radical scavenging rate (%) was obtained using Equation 1 below, and the results are shown in Table 7 below.

[Equation 1]

Samples (final concentrations are all 0.005% except control) Free radical scavenging rate (%) Control group (no addition) 0 YM badge 9.5 MRS Badge 10.3 BL badge 11.1 Ascorbic acid 42.7 Fermented Yeast Extract of Licorice 43.0 Lactic Acid Bacteria Fermented Extract of Ginseng 44.2 Example 1 65.3 Example 2 67.5 Example 3 64.2 Example 4 67.9 Example 5 65.1 Example 6 75.2 Example 7 60.1 Example 8 77.9 Example 9 67.1 Example 10 75.4

As shown in Table 7, Examples 1 to 10 of the present invention has a free radical scavenging rate compared to yeast fermentation extract of licorice, lactic acid bacteria fermentation extract of ginseng or ascorbic acid used as a basic medium or a known antioxidant All high.

Example 11 and Comparative Example 1

Using Example 1 to prepare a skin external ointment according to the formula shown in Table 8. (Unit: wt%)

division Example 11 Comparative Example 1 Component of Example 1 10 - Diethyl sebacate 8 8 Prepayment 5 5 Polyoxyethylene oleyl ether phosphate 6 6 Sodium benzoate Quantity Quantity vaseline To 100 To 100

Example 12 and Comparative Example 2

Using Example 3, the cream was prepared according to the formulation shown in Table 9 below.

division Example 12 Comparative Example 2 Component of Example 3 1.0 - Stearic acid 15.0 15.0 Cetanol 1.0 1.0 Potassium hydroxide 0.7 0.7 glycerin 5.0 5.0 Propylene glycol 3.0 3.0 antiseptic Quantity Quantity incense Quantity Quantity Purified water To 100 To 100

Example 13 and Comparative Example 3

Using the above Example 6 was prepared in the softener according to the prescription of Table 10. (Unit is weight%)

division Example 13 Comparative Example 3 Component of Example 6 0.2 - ethanol 10.0 10.0 Polylauric acid polyoxyethylene sorbitan 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 glycerin 5.0 5.0 1,3-butylene glycol 6.0 6.0 incense Quantity Quantity Pigment Quantity Quantity Purified water To 100 To 100

Example 14 and Comparative Example 4

Using Example 8, nutritional longevity was prepared according to the formula shown in Table 11 below. (Unit is wt%.)

division Example 14 Comparative Example 4 Ingredients of Example 8 0.1 - Waselin 2.0 2.0 Sesquioleate sorbitan 0.8 0.8 Polyoxyethylene oleylethyl 1.2 1.2 Methyl paraoxybenzoate Quantity Quantity Propylene glycol 5.0 5.0 ethanol 3.2 3.2 Carboxy Vinyl Polymer 18.0 18.0 Potassium hydroxide 0.1 0.1 Pigment Quantity Quantity incense Quantity Quantity Purified water To 100 To 100

Example 15 and Comparative Example 5

The pack was prepared according to the formulation of Table 12 using Example 9.

division Example 15 Comparative Example 5 Ingredients of Example 9 0.5 - glycerin 5.0 5.0 Propylene glycol 4.0 4.0 Polyvinyl alcohol 15.0 15.0 ethanol 8.0 8.0 Polyoxyethylene oleylethyl 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 incense Quantity Quantity Pigment Quantity Quantity Purified water To 100 To 100

Example 16 and Comparative Example 6

Essence was prepared according to the formulation of Table 13 below using Example 10.

division Example 16 Comparative Example 6 Ingredients of Example 10 5.0 - Propylene glycol 10.0 10.0 glycerin 10.0 10.0 Sodium hyaluronate aqueous solution (1%) 5.0 5.0 ethanol 5.0 5.0 Polyoxyethylene Cured Castor Oil 1.0 1.0 Methyl paraoxybenzoate 0.1 0.1 incense Quantity Quantity Purified water To 100 To 100

Experimental Example 2. Anti-aging Effect

The anti-aging effect of the cosmetic composition of the present invention was verified as follows.

Experiments were performed using 20 hairless mice of 15 to 20 weeks of age per each experimental group. Ultraviolet rays were irradiated to the back of the hairless mice by using an ultraviolet irradiator (UV simulator, US) at a dose of 2mJ / cm 2, which was performed twice a week for 4 weeks to induce photoaging of the skin of the mice. Thereafter, the cosmetics of Examples 11 to 16 were applied to one side of the hairless mice and the like twice a day for 2 weeks, and the other side was coated with the cosmetics of Comparative Examples 1 to 6 for comparison. At this time, the packs of Example 15 and Comparative Example 5 were removed 15 minutes after the application. Two weeks later, the aging-releasing effect was observed by the relative comparison with the naked eye, and evaluated in three stages of distinct effects, effects, and no differences, and the results are shown in Table 14.

division Distinct Effect (People) Effective (persons) No difference (persons) Example 11 7 8 5 Example 12 5 9 6 Example 13 5 10 5 Example 14 8 7 5 Example 15 7 8 5 Example 16 6 9 5

As shown in Table 14, the cosmetics prepared according to Examples 11 to 16 exhibited an anti-aging effect on at least 14 or more of 20 subjects, and in particular, do not show any side effects in the skin, which is safe for the human body. It was found that the effect is an anti-aging cosmetic having an excellent antioxidant function.

The cosmetic composition having an antioxidant activity according to the present invention is safe for the skin and has excellent skin anti-aging and antioxidant effects and sustainability of the effect.

Claims (11)

Antioxidant activity, characterized in that it comprises fermented extract extracted by fermenting mushroom fungi fermentation of at least one species selected from the group consisting of licorice, Angelica, ginseng, gold, and Baekbongryeong as yeast, lactic acid bacteria or bifidus bacteria as an active ingredient Cosmetic composition having a. The method of claim 1, Cosmetic composition having an antioxidant activity, characterized in that the total amount of the fermented extract content is 0.0001 to 20% by weight. The method of claim 1, The mushroom is an antioxidant characterized in that at least one selected from the group consisting of chaga (Inonnotus-obliquus), mushroom (phellinus linteus), cordyceps fungi (Cordyceps sinensis, Paecilomyces japonica ), and Ganoderma lucidum Cosmetic composition having activity. The method of claim 1, The yeast is a cosmetic composition having an antioxidant activity, characterized in that at least one selected from the group consisting of Saccharomyces cerevisiae , Schizosaccharomyces ellipsoids and Saccharomyces boulardii . The method of claim 1, The lactic acid bacteria Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus reuteri and Lactobacillus confusus, Lactobacillus fermentum, Lactobacillus fermentum having at least one cosmetic composition selected from the group consisting of anti-oxidant. The method of claim 1, The Bifidobacteria is a cosmetic composition having an antioxidant activity, characterized in that at least one selected from the group consisting of Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum and Bifidobacterium infantis . The method of claim 1, The cosmetic composition having an antioxidant activity is a cosmetic composition having an antioxidant activity, characterized in that the skin external ointment, supple cosmetics, nourishing cosmetics, nutrition cream, massage cream, essence, pack, emulsion, or oil gel formulation. The method of claim 1, Cosmetic composition having an antioxidant activity, characterized in that the cosmetic composition further comprises at least one additive selected from the group consisting of oil, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, and fragrances. Composition. a) extracting mushroom fungi fermenting mushrooms by fermenting a medicinal herb selected from the group consisting of licorice, donkey, ginseng, gold, and Baekbongnyeong; b) fermenting the mushroom fermented product of step a) with yeast, lactic acid bacteria or bifidus bacteria; And c) separating the fermentation extract from the fermentation of step b) Method for producing a cosmetic raw material having an antioxidant activity, characterized in that it comprises a. The method of claim 9, The method of producing a cosmetic raw material having an antioxidant activity, characterized in that the mushroom fermentation of step a) is a solid fermentation. The method of claim 9, Yeast, lactic acid bacteria or bifidus fermentation of step b) is a method of producing a cosmetic raw material having an antioxidant activity, characterized in that the liquid fermentation.