KR101423573B1 - Cosmetic composition having antioxidation activity - Google Patents

Abstract

The present invention relates to a cosmetic composition having an antioxidant activity, and more particularly to a cosmetic composition having an antioxidant activity, and more particularly to a cosmetic composition having an antioxidant activity, Mushroom, green tea, sorghum, soybean sprout, lily, and citric acid as an active ingredient. The present invention also relates to a cosmetic composition having antioxidant activity. The cosmetic composition having an antioxidative activity according to the present invention is excellent in skin-aging and antioxidative effect and persistence of effect, while being safe for skin.

Cosmetics, fermentation extract, antioxidant, anti-aging

Description

[0001] Cosmetic composition having antioxidation activity [0002]

The present invention relates to a cosmetic composition having antioxidant activity, and more particularly, to a cosmetic composition having antioxidant activity that is safe to the skin and has excellent anti-aging and antioxidant effects and long-lasting effects.

In general, there are many ways to explain skin aging, but the free radical theory that skin is aged by free radicals caused by various causes outside the body is accepted as the most valid theory. Free radicals are highly chemically reactive chemicals that are produced in the body by external factors such as ultraviolet radiation, radiation, exhaust gas, tobacco smoke, stress, and metabolism of mitochondria and leukocytes in the body , Which leads to the peroxidation of the lipid component of the cell membrane through a multistage chain reaction.

The major part of the free radicals associated with skin aging is reactive oxygen species (ROS), which include superoxide radicals, hydrogen peroxide (H ₂ O ₂ ), hydroxy radicals ), Singlet oxygen (singlet oxygen), and the like. The lipid peroxidation caused by these causes various active oxygen species of radicals, peroxides, aldehydes and epoxides, damaging DNA, RNA, proteins, cell membranes and cell structure.

These reactive oxygen species are thought to be the main cause of cancer, tissue damage, and aging (Black HS, Photochemphotobiol. 46 (2): 213 (1987)). For example, capillary peroxidation, which constitutes a cell, can be characterized by changes in structure and fluidity of the cell membrane, increased permeability of cytoplasmic material, leakage of lyceosomal enzyme, inactivation of membrane enzymes and transport proteins, Chain cleavage, DNA damage, and mutation. Among these reactions, peroxidation of skin constitutive membranes and modification of dendritic proteins are known to be most closely related to skin aging.

Aerobic organisms have protective mechanisms to prevent the production of these oxidants and the lipid peroxidation. These protective mechanisms include enzymes such as superoxide dismutase (SOD) and catalase, tocopherol (vitamin E), beta- carotene, ascorbic acid (vitamin C) And lipid soluble and water soluble antioxidants or radical scavengers such as glutathione.

On the other hand, skin antioxidants known so far have been developed in anticipation of the following effects. First, it provides moisture to the skin, second, antioxidant effect, third, promotes the proliferation of fibroblasts involved in the activation of skin cells, and fourth, an enzyme that accelerates the production of wrinkles and loss of elasticity by decomposing collagen and elastin, Collagenase and elastase, and fifth, promotion of collagen and elastase synthesis.

As described above, since free radical oxygen or reactive oxygen species are highly related to skin aging, conventionally, superoxide dismutase, vitamin E derivatives, vitamin C and derivatives thereof, and flavonoids have been added to cosmetics There have been a lot of efforts to achieve the skin aging prevention effect by mixing the above ingredients. However, when the above ingredients are mixed in cosmetics, safety, stability, and the like are poor, and it is difficult to expect a substantial effect.

Conventionally, a substance such as BHT (2,6-di-tertiarybutyl-4-methylphenol), BHA (2-tertbutyl-4-methoxyphenol), or PG (3,4,5-terhydroxy benzoic acid propyl ester) Used a lot. However, there are disadvantages such as consumers' rejection of such synthetic antioxidants as their diet and living standards are improved. In particular, BHT, which is the most widely used synthetic antioxidant in the past, has been reported to cause cancer when consumed at 50 mg / kg / day or more. Therefore, although researches to find a substance having antioxidant activity from natural materials such as shim products were carried out vigorously, most of them had antioxidant ability which did not meet the expectation.

Therefore, in order to solve the problems of the prior art described above, there is a continuing need for the development of products and raw materials having superior ability to remove active oxygen.

On the other hand, fermentation is a process of decomposing organic matter using enzymes possessed by microorganisms. Fermentation and corruption proceed by a similar process, but decomposition is called fermentation when substances that are useful for our lives are made, and when they make odorous or harmful substances, it is called corruption. Therefore, if the herb medicine is fermented using microorganisms, the toxicity of the herb medicine is reduced, the low molecular weight is easily digested, and the transdermal absorption upon application to the skin becomes easy, and the bioavailability is improved.

The herbal medicines themselves can exhibit pharmacological effects, but the pharmacological effects can be further strengthened by fermentation or harmful components can be produced by the metabolism of microorganisms, so that a new pharmacological effect can be exhibited which is not present in the herbal medicine before fermentation.

Accordingly, it is an object of the present invention to provide a cosmetic composition having antioxidative activity that is safe to the skin and has excellent anti-aging and antioxidant effects and persistence of the effect.

Another object of the present invention is to provide a cosmetic composition having an antioxidative activity that further enhances the pharmacological effect of each medicinal substance through fermentation and further enhances collagen synthesis promoting effect.

In order to accomplish the above object, the present invention provides a method for producing a green tea, which comprises the steps of: preparing a green tea, A cosmetic composition having an antioxidative activity comprising, as an active ingredient, a fermented extract of a medicinal substance selected from the group consisting of ginseng root, soybean ginseng root, lily, and safflower.

The fermentation extract can be obtained by adding a medicinal substance to yeast, lactic acid bacteria or bifidobacteria and culturing the same.

The fermented extract can be obtained by inoculating fungi into a medicinal material and solid fermentation.

The medicinal fermentation extract is preferably contained in the cosmetic composition in an amount of 0.0001 to 10% by weight.

The cosmetic composition may be formulated into ointment for external use, softening longevity, nutritional lotion, nutritional cream, massage cream, essence, pack, emulsion, oil gel and the like.

Hereinafter, the cosmetic composition having the antioxidative activity of the present invention will be described in more detail.

The medicinal plant extracts described in the present invention can be used in a variety of fields such as health, seomyeotai, sugar pumpkin, hecke, doin, seokchangpo, truffle, chrysanthemum, ogapi, raw paper, Refers to an extract obtained by extracting a medicinal substance selected from at least one kind selected from the group consisting of sorghum, soybean sprout, lily, and citric acid according to a conventional method. Also, the medicinal fermentation extract means a secondary extract obtained by inoculating and culturing the above-mentioned medicinal plant extract into yeast, lactic acid bacteria or bifidus microorganisms, or obtained by solid fermentation by adding fungi to commercially available medicinal products or pulverized products.

The present inventors have conducted studies to develop fermented Chinese medicinal materials having excellent antioxidant properties, and as a result, they have found that they can be used in a variety of fields such as health, seomyeottae, gangwon, Fermented broths such as Cheongpiae, Cheonpiyi, Chinamushi, Green tea, Ginseng, Soybean sprout, Liliaceae, and Seokho have remarkably superior antioxidative effects than the respective extracts.

The cosmetic composition having antioxidant activity according to the present invention is useful as an antioxidant for cosmetics, cosmetics, cosmetics, pharmaceuticals, cosmetics, pharmaceuticals, cosmetics, As an active ingredient, a fermented extract of a medicinal substance selected from the group consisting of ginseng, soybean ginseng, lily, and safflower.

The health (Zingiber officinale) is a word for dried ginger, used for the treatment of 胃寒, vomiting and diarrhea.

Rhynchosia nulubilis is a type of black bean, which has a yellow-tanned roe and a blue-tailed rosemary. Seomok-tae is also called native yakon because it grows in mountains. Seomoktae (鼠 太 太) is a name made by saying that it looks like black eyes. It is known as a sweet potato that is eaten in rice in Seomoktae or has excellent detoxification effect from ancient times. There are several records of Seomoktae, which is a bean, which is a bean.

The sugarcane (Cucurbita pepo) is a perennial ginkgo. Fruits of lycopodium are rich in carotenoids and are a source of vitamin A in winter. Adenine, asparagine, trigonelline, phytosterin, hypoglycemic substances and the like. It also has diuretic action and accelerates the excretion of salt. Therefore, there is an auxiliary effect on nephritis and hepatitis. Pumpkin seeds contain the amino acid Cucurbitin, acidic, neutral, basic amino acids (especially lysine). Vitamins B, C and E and urease, an enzyme, contain about 34% of the oil. The oil is linoleic acid, oleic acid, palmitic acid, and glyceride of stearic acid.

Codonopsis lanceolata, also known as cedarwood, is a perennial herbaceous perennial herbaceous bloom with a length of about 2 m and can be identified with its unique fragrance when it reaches near its native habitat. It removes the waste heat from the Hanchi and uses it for the blood clot when there is fever, dry mouth, fever in the lung, cough and sputum. In the private sector, it is used as a gangjeongjeong medicine.

It is a genus that breaks the hard seed of peach which is Prunus persica. The seeds include Ancestor, Amigallin, and so on. In the private sector, the leafy branches are placed in a cauldron, and the bath is bathed in fever, eczema. Doine is an anti-inflammatory medicinal product, used for 3 to 5 g per day for irregular menstruation and abdominal pain.

The Acorus tatarinowii is a perennial herb that is 70-100 cm high from the genus Acanthus. It is used as a diuretic in sedative, anal, and placebo. It is used for mental disorder, tinnitus, headache, arthritis, gastritis, duodenal ulcer, dyspepsia, chronic bronchitis and repetition. It is also used as a hot water source for women's cold stomachache and back pain.

The tuber melanosporum is an underground mushroom of the winged ginseng and is a mushroom completely different from the mushroom belonging to the bracts. It is not found in Korea yet.

The Angelica MAKINO is a perennial herb belonging to the falciparum family. It is commonly called asymilobia or Shinchigoso in Japan and green herb in China. If you break the stem of the kyunchok, yellow juice comes out, and the chalcone and coumarin, which are contained in this juice, have an effect of suppressing the cancer. It is used in the treatment of diabetes because of the hypoglycemic effect, and has the effect of removing cholesterol and normalizing the blood pressure without lowering or increasing the blood pressure unconditionally, and thus has an excellent effect on arteriosclerosis, hypertension and hypotension. In addition, organic germanium and chlorophyll help oxygen supply to the cells and improve blood circulation, thus helping to prevent and treat anemia and prevent congestion (鬱 血).

Acanthopanax gracilistylus belongs to the Araliaceae, a deciduous broad-leaved shrub with a height of 2 ~ 5 m, with 3 ~ 5 leaves, horny and long bracts, with a thorn in the stem, It blooms green in August and ripens black in September and October. The origin of the medicine is the roots, stems, and shells of the oak and other plants. It has attracted worldwide attention as a new medicinal plant due to its wide pharmacological effect. It has been listed as a product of the Divine Husbandman's Medicinal Mentality since ancient times. It has been widely used as a medicinal herb, (祛风濕), and the blood (动血) efficacy, such as witwoongpyeong (寒 濕 痛 痛), gyeolbyeol (筋 弱 weak), plump (痛), rheumatoid arthritis, backache (腰痛), degenerative joint syndrome, It is used for dysentery, edema, 脚气, and cyanoma. The daily consumption is 6 ~ 12g and it can be used in a variety of foods such as ogalpisan, ogafiban, ogaju, oilseed powder, nutritional hotpot, It is used for the prescription raw materials such as the 脊 湯.

Polygala tenuifolia is a medicinal plant with roots, and its taste is spicy and warm. When I am surprised and chesty, when I cough with phlegm, I am used to amnesia. Sedation, hypnotic action, radiant action, sputum sagging action, hemolytic action and so on.

The above-mentioned Cistanche deserticola is a parasitic duckling on the root of a ducklings, which is used as a substitute for a seedling. It is an annual plant with a height of about 30 centimeters. Root stems are tuberous stems. Stems are fleshy pillars (fleshy plants). Leaves are yellowish brown scales, and in summer, dark purple flowers bloom in a spike shape. It is parasitic on the alder tree of the high mountain region. Gangjeong Gangjeong medicine is used for vomiting, oil wells, dizziness, low back pain, blood clots, fever, constipation. It is also used for nerve tonic drugs, cardiac activity enhancers, cystitis, and hematuria. It is also used to make ulcers by making plaster with rosin. Jong - Yong is less effective than.

Phaseolus angularis has been widely used as a medicine to eliminate toxins from red beans.

Gleditsia japonica is a bark of pine nuts and has two types of pods: long pod and long pod pod. Its nature is temperate and tasty, and it is poisonous and is used to remove paroxysmal, migraine headaches, paralysis, pesticides, or sputum. Chauffeur has the main efficacy of stopping coughing, balancing breathing and getting rid of sputum.

The Saussurea involucrate is said to be an extremely valuable medicinal herb that also appears in lotus flowers bloomed on new stiffness and martial arts novels.

The citrus nobilis is made of citrus peel which has not been used for a long time. The ripe part is removed from the dermis and the white part inside the shell is removed, and the remaining part is called citrus red. . The skin of the less ripe mandarin orange, which has a strong ability to dissipate the muscles, is good for relieving the stomach and sore in the side due to excessive stress.

The locust (Prunus armeniaca) is dried apricot seed. Amigalline, fetish, protein, and the main component of oil is olein. Amigalline acts on the respiratory center and cough center to cause coughing. Passengers mainly drink cough, seawater, asthma, and breath feces 3 to 6 g dalyeoseo. With cinnamon, the effect becomes stronger with the moon.

The Schizophyllum commune is 1-3 cm in diameter and has no stem. It attaches to the body as part of the side or back side of the gymnosperm, and it has a fan shape or a round shape, sometimes cracked like a palm. The surface is rough-haired and white-gray or gray-brown. The wrinkles are white-gray or light salmon colored or purple, and the margins are divided vertically and appear to overlap two sheets. The flesh is leather, and when it is dried, it is cured. When it is immersed in water, it becomes undone. It is known that the polysaccharide originating from the skirt mushroom has a healing effect on burns and wounds.

The green tea (Camellia sinensis) is produced by heating and moisture-evaporating young leaves of an unfermented tea leaf. From prevention of adult diseases such as hypertension and diabetes to dieting, the efficacy of green tea is unrivaled.

The ginseng (Nardostachys chinensis) is a plant of Matari, which grows in the Himalayan mountain range. There are about 2% of essential oil in root. Essential oil components include Atamanson, terpenes, coumarin derivatives Orocellol, and Yata Mansin. It is used as a spice and a directional food.

The soybean gland (Glycine max) is a large bean sprout that regulates muscle spasms, cuts off the number of sperms, and heals the lice.

The lily (Lilium brownie) is a herbaceous perennial of Liliaceae having a height of about 1 to 1.5 m. It is called lily in the summer when the reddish-purple flower with black spots blooms, and the blossoming of the white lily is called lily. We take bulbs in autumn and use them as medicine. It is used when the cough and blood are shed without the phlegm, the nervous breakdown after the fever, and the sleep can not be achieved.

The Bupleurum chinense is herbaceous plant having a height of 40 to 70 cm. Chronic nephritis, hepatitis and so on. Peach seeds are more effective when used together. The epidermal action of Seoh is used in acute cold medicine and its anti-inflammatory action is effective in removing inflammation in the body. Antipyretic, analgesic, and anti-inflammatory medicines, and is also used for medicines, hepatitis, pleurisy, dysmenorrhea, gastritis, jaundice and hepatitis.

The medicinal materials may be prepared by a conventional method, for example, by extracting with an organic solvent such as distilled water, anhydrous or low-boiling alcohol having 1 to 4 carbon atoms, concentrating under reduced pressure and then drying to obtain a first extract . Specifically, there are a variety of products that are commercially available as herbal medicines, such as health food, seomyeotai, sugar pumpkin, doduk, doin, seokchonpo, tongue mushroom, Soybean hulls, lilies or citrus fruits are purchased and finely pulverized, and 5 to 20 volumes of water or an organic solvent such as anhydrous or low-boiling alcohol having 1 to 4 carbon atoms, based on the dry weight of the pulverized product, Heat the extract to 100 ℃ for 1 to 5 hours to extract the extracts of the medicines. After filtration with a filter cloth, the residue is further extracted one or more times by the same method. The extracts are combined, concentrated under reduced pressure, and lyophilized or spray dried to obtain a dry extract.

The herbal extracts obtained as described above may be added to yeast, lactic acid bacteria or bifidus bacteria and cultured to obtain a final fermented extract. At this time, it is preferable that the medicinal plant extract is added to the basic medium of yeast, lactic acid bacteria or bifidobacteria in an amount of 1 to 20% by weight. In addition, the pharmaceutical fermentation extract may be obtained by inoculating the commercially available medicinal product itself or a slice or powder into a fungi, followed by solid fermentation.

The yeast may be Saccharomyces cerevisiae , Schizosaccharomyces ellipsoids , Saccharomyces boulardii or the like.

The lactic acid bacteria may be selected from the group consisting of Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus confusus, Lactobacillus fermentum and Lactobacillus brevis .

Examples of the bifidobacteria include Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum , Bifidobacterium infantis , and the like.

The fungus can be used for medicinal mushroom fungi such as Inonnotus-obliquus, Cordycepsps sinensis, Paecilomyces japonica , Tricholoma matsutake, phellinus linteus and Ganoderma lucidum. .

More specifically, in the case of using yeast as a microorganism, a certain concentration (about 10 ⁵ to 10 ⁷ cells / mL) is used for the health, Seomyeontae, sugar pumpkin, Doduk, doin, seokchangpo, tongue mushroom, Yeast was inoculated in a primary culture medium (YM medium) containing the first extracts of Pseudomonas aeruginosa, Pseudomonas aeruginosa, Chopseong, Cheonsoonryeon, Cheongpijeong, Cheonmyung, Green tea, 6.5, a temperature of 25 to 35 DEG C, and aeration amount of 0.05 to 0.5 VVM. Thereafter, the fermentation broth is centrifuged to remove the polymer precipitate and the yeast body, and the concentrate is concentrated under reduced pressure at 60 ° C in a distillation apparatus equipped with a cooling condenser, followed by lyophilization, thereby obtaining each of the fermentation extracts of medicinal plants.

When lactic acid bacteria are used as a microorganism, the concentration is about 10 ⁵ to 10 ⁷ cells / mL, and the concentration is about 10 ⁵ to 10 ⁷ cells / mL. Lactic acid bacteria were inoculated in a primary culture medium (MRS medium) containing the first extracts of Phellodendron spp., Cheongpiju, Cheonmyung, Green tea, Ginseng, Soybean spp., Lily and Seiho, ℃ and aeration volume of 1 VVM for 24 ~ 120 hours. Thereafter, the fermentation broth is centrifuged to remove the polymer precipitate and the lactic acid bacterium, and the concentrate is concentrated under reduced pressure at 60 ° C in a distilling apparatus equipped with a cooling condenser, followed by freeze-drying, thereby obtaining each of the fermentation extracts of medicinal plants.

When bifidobacteria are used as microorganisms, they are used at a constant concentration (about 10 ⁵ to 10 ⁷ cells / mL), and they can be used in a variety of applications such as health, seomyeotai, sugar pumpkin, Bifidobacteria were inoculated on a primary culture medium (BL medium) containing primary extracts of Cheonan, Cheongpijeong, Cheonmyung, Cheonmachung, Green tea, And incubated for 24 to 120 hours under anaerobic conditions. Thereafter, the fermentation broth is centrifuged to remove the polymer precipitate and bifidus microbial cells, and the suspension is concentrated in a distillation apparatus equipped with a cooling condenser at 60 ° C and freeze-dried to obtain the respective fermentation extracts of medicinal products.

In case of using fungi as a microorganism, it is preferable to use fungi as a microorganism in the case of using fungi such as health, Seomyeontae, sugar pumpkin, Deokduk, doin, seokchangpo, truffle mushroom, 1 to 10% by weight of fungi were inoculated into each of green tea, ginseng root, soybean soup, soybean lily, lysine and Seiho itself or pieces and pulverized pulverized product, and solid fermentation was conducted for 60 days at 18 to 25 캜 and 60 to 70% Can be obtained.

When the free radical scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) was measured, the extracts of general herbal extracts And exhibits a remarkably superior antioxidative effect. In addition, when the cosmetic composition is prepared by using the above fermented extracts of medicinal materials, it is safe to the skin without any side effects when applied to human skin, exhibits skin aging and antioxidant effect.

The obtained medicinal fermented extract may be contained in an amount of 0.0001 to 10% by weight, more preferably 0.0005 to 10% by weight, based on the cosmetic composition having the antioxidative activity of the present invention. When the content is less than 0.0001% by weight, no remarkable effect can be expected. When the content is more than 10% by weight, there is no evidence of a marked effect due to the increase of the content.

The formulation of the cosmetic composition having the antioxidative activity containing the above fermented extract of the present invention as an active ingredient is not particularly limited and may be appropriately selected according to the purpose. For example, the cosmetic composition of the present invention may be formulated into ointment for external use, softening longevity, nutritional lotion, nutritional cream, massage cream, essence, pack, emulsion, oil gel and the like. In this case, the ointment for external use on skin may be prepared so as to contain 50 to 97% by weight of vaseline and 0.1 to 5% by weight of polyoxyethylene oleyl-ether phosphate in addition to the medicinal fermentation extract of the present invention. 1 to 10% by weight of polyhydric alcohols (propylene glycol, glycerin, etc.) and 0.05 to 2% by weight of a surfactant (polyethylene oleyl ether, polyoxyethylene hardened castor oil, etc.). The nutritional lotion and nutritional cream may contain 5 to 20% by weight of oils (squalane, petrolatum, octyldodecanol, etc.) and 3 to 15% by weight of wax components (cetanol, stearyl alcohol, %, And the essence can be prepared by adding 5 to 30% by weight of polyhydric alcohols such as glycerin, propylene glycol and the like in addition to the fermented extract of the present invention. The massage cream is prepared by adding 30 to 70% by weight of an oil such as liquid paraffin, petrolatum, isononylisononanoate and the like in addition to the medicinal fermented extract of the present invention. In addition to the fermented extract of the present invention, the pack contains polyvinyl alcohol A peel off pack or a general emulsifiable cosmetic composition containing a weight% of a pharmacologically active ingredient of the present invention is characterized in that the pharmaceutical composition of the present invention contains a 5 to 30 wt% pigment such as kaolin, talc, zinc oxide, titanium dioxide, ) Pack.

In addition, the cosmetic composition having antioxidative activity of the present invention may contain, in addition to the above-described ingredients, the components described above, such as oil, water, surfactant, moisturizer, lower alcohol, thickener, chelating agent, dye, preservative, And the like can be appropriately used in combination as needed.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following examples.

[Example]

Example 1

Healthy, Seomoktae, Pumpkin, Duckuk, Doin, Seokchangpo, Truffle, Chrysanthemum, Oganegi, Yeji, Seongjongyong, 100 g of each of the crushed and ground pulverized products was put into purified water or 3 L of 80% methanol, and the mixture was boiled for 3 hours in a reflux condenser equipped with a condenser. After filtration through a 100-mesh filter cloth, the residue was extracted one more time by the same method. Each of the extracts was combined and filtered through a filter paper of Whatman No. 2 at room temperature to remove insoluble matters. The extract was concentrated under reduced pressure at 60 ° C in a distillation apparatus equipped with a cooling condenser, followed by lyophilization, ≪ / RTI > Table 1 below shows the dry weight of the extract per 100 g of medicament according to the extraction solvent.

Medicinal 80% methanol Purified water health 7.5 6.8 Seomok-tae 6.5 5.7 Sugar pumpkin 8.1 7.4 Doduk 8.2 7.5 Doin 7.3 6.7 Seokchangpo 8.3 6.3 Truffle 8.2 7.9 Chinese 6.9 6.1 Ogaki 8.1 6.4 Origin 8.5 6.8 Breeding 6.8 6.1 Red Dog 7.7 7.0 Chow 7.5 6.7 Cheon San 4.8 4.3 Cheongpyi 6.5 5.9 Passerine 6.5 6.1 Skirt mushroom 8.6 7.8 green tea 6.1 5.3 Gam-cho 5.7 5.1 Soybean heaven 7.6 6.8 Lily 6.3 5.7 Seoho 7.2 6.6

Examples 2 to 23

Each of the herbal extracts obtained using 80% methanol was added to 5% by weight of YM medium as a basic medium for Saccharomyces cerevisiae, which was a yeast, and fermented.

Specifically, Saccharomyces cerevisiae was inoculated into a yeast basic medium (YM medium) containing 5 wt% of each herbal extract at a concentration of about 10 ⁵ to 10 ⁷ cells / mL, Lt; 0 > C, and aeration amount of 0.05-0.5 VVM. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and the yeast organism, and then concentrated under reduced pressure at 60 DEG C in a distilling apparatus equipped with a cooling condenser, followed by freeze-drying to obtain a final fermented yeast fermentation product. The dry weight of the dried fermented product of the medicinal yeast is shown in Table 2 below.

Medicinal Dry weight (g) of fermentation broth Example 2 (Health) 5.92 Example 3 (Seomyeongtae) 6.07 Example 4 (sugar cane) 5.87 Example 5 (Dodec) 6.17 Example 6 (Doine) 5.95 Example 7 (Seok Changpo) 6.04 Example 8 (truffle) 6.05 Example 9 (fresh cherry) 5.88 Example 10 (Ogapi) 6.08 Example 11 (Paper) 6.16 Example 12 For breeding) 5.87 Example 13 (Reddish head) 5.91 Example 14 (Chosho) 6.22 Example 15 6.11 Example 16 (CheongPy) 6.03 Example 17 (pass) 5.93 Example 18 (skimmed mushroom) 6.05 Example 19 (green tea) 5.89 Example 20 (Gam-sung) 5.85 Example 21 (soybean yellow) 6.25 Example 22 (Lily) 5.87 Example 23 (Seohan) 6.12

Examples 24 to 45

Each of the herbal extracts obtained by using 80% methanol was added to 5% by weight of MRS medium as a basic medium for Lactobacillus acidophilus, and fermented.

Specifically, Lactobacillus acidophilus was inoculated into a lactic acid bacterial basic medium (MRS medium) containing 5 wt% of each of the herbal extracts at a concentration of about 10 ⁵ to 10 ⁷ cells / mL, and then pH 6.5 to 7.5 and temperature 35 to 38 , And a ventilation amount of 1 VVM was maintained for 48 hours. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and the lactic acid bacteria, followed by concentration under reduced pressure at 60 DEG C in a distilling apparatus equipped with a cooling condenser, followed by lyophilization to obtain a final fermented product of lactic acid bacteria. The dry weight of the fermented product of the medicinal lactic acid bacteria is shown in Table 3 below.

Medicinal Dry weight (g) of fermentation broth Example 24 (Health) 6.11 Example 25 (Seomyeongtae) 6.22 Example 26 (sugar cane) 6.02 Example 27 (Dodec) 6.12 Example 28 (Doine) 6.01 Example 29 (Seok Changpo) 6.12 Example 30 (truffle) 5.99 Example 31 (fresh cherry) 6.12 Example 32 (Ogapi) 6.27 Example 33 (Paper) 5.97 Example 34 (for breeding) 6.00 Example 35 (Red head) 5.96 Example 36 (Chosho) 6.11 Example 37 (Cheon San Gyunryeon) 6.05 Example 38 (CheongPy) 6.10 Example 39 (action) 6.04 Example 40 (skimmed mushroom) 6.11 Example 41 (green tea) 5.93 Example 42 (Gamma-Ray) 5.89 Example 43 (soybean yellow) 6.16 Example 44 (Lily) 5.96 Example 45 (SE) 6.23

Examples 46 to 67

Each of the herbal extracts obtained by using 80% methanol was added to the BL medium as a basic medium for bifidobacter bifidobacterium longum at 5 wt% and fermented.

Specifically, Bifidobacterium longum was inoculated into a lactic acid bacterial basic medium (BL medium) containing 5% by weight of each of the herbal extracts at a concentration of about 10 ⁵ to 10 ⁷ cells / mL, and then pH 6.5-7.5, temperature 35-38 ℃, and anaerobic culture conditions. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and bifidus microbial cells. The microbial precipitate and bifidus microbial cells were removed by a distillation apparatus equipped with a cooling condenser, followed by concentration under reduced pressure at 60 DEG C and freeze-drying to obtain a final fermented bifidus microorganism bacterium. The dry weight of the dried fermented product of the medicinal bifidus bacterium is shown in Table 4 below.

Medicinal Dry weight (g) of fermentation broth Example 46 (Health) 6.17 Example 47 (Seomyeongtae) 5.96 Example 48 (sugar amber) 6.11 Example 49 (Dodec) 5.89 Example 50 (Diane) 6.03 Example 51 (Seok Changpo) 6.04 Example 52 (truffle) 6.04 Example 53 (fresh cherry) 6.17 Example 54 (ogapi) 6.11 Example 55 (Paper) 6.04 Example 56 (for breeding) 6.09 Example 57 (Rats head) 6.05 Example 58 (crude oil) 6.15 Example 59 (Cheonsoon Suksilon) 6.13 Example 60 (CheongPy) 5.97 Example 61 (action) 6.19 Example 62 (skimmed mushroom) 5.82 Example 63 (green tea) 5.99 Example 64 (Gamma-Ray) 6.01 Example 65 (soybean yellow) 6.10 Example 66 (Lily) 6.03 Example 67 (SE) 6.02

Examples 68 to 89

The medicinal materials used in Table 1 were sectioned and added with 5% by weight of fungi for solid fermentation.

Specifically, 200 g of each of the medicinal materials was used as a slice, Paecilomyces japonica was inoculated at 5% by weight under constant humidity, and the mixture was fermented for 60 days at a temperature of 18 to 25 캜 and a humidity of 60 to 70% g was added to 3 L of 80% methanol, and the mixture was boiled for 3 hours in a reflux condenser equipped with a condenser. After filtration with a 100 mesh filter cloth, the residue was extracted one more time by the same method. Each of the extracts was combined and filtered through a filter paper of Whatman No. 2 at room temperature to remove insoluble matter. The extract was concentrated under reduced pressure at 60 ° C in a distillation apparatus equipped with a cooling condenser and then lyophilized to obtain a final fermented product of the medicinal fungus. The dry weight of the dried fermented product of the medicinal fungus is shown in Table 5 below.

Medicinal Dry weight (g) of fermentation broth Example 68 (Health) 6.75 Example 69 (Seomyeongtae) 5.63 Example 70 (sugar amber) 7.03 Example 71 (Dodec) 7.15 Example 72 (Diane) 6.41 Example 73 (Seocheonpo) 6.78 Example 74 (truffle) 6.80 Example 75 (fresh cherry) 6.12 Example 76 (Ogapi) 6.33 Example 77 (paper) 6.54 Example 78 (for breeding) 6.05 Example 79 (Reddish head) 6.87 Example 80 (crude oil) 6.63 Example 81 3.87 Example 82 (CheongPy) 5.77 Example 83 (action) 5.56 Example 84 (skimmed mushroom) 7.88 Example 85 (Green tea) 5.57 Example 86 (Gam-sungang) 5.12 Example 87 (soybean yellow) 6.89 Example 88 (Lily) 5.52 Example 89 (SE) 6.47

Experimental Example 1. Free radical scavenging rate

The free radical scavenging activity was measured to confirm the antioxidative activity of each of the medicinal substance itself and the fermented extract of the medicinal materials of Examples 2 to 89 described above.

For each of the test samples, the respective medicinal materials themselves and the fermented extracts of Examples 2 to 89 were used, and the free radical scavenging activity was measured using DPPH. DPPH was purchased from Sigma Co. (Sigma Co., Ltd, USA) and used. First, a standard DPPH solution with a concentration of 400 mu m was prepared. Ethanol was added to each of the medicinal materials themselves, ascorbic acid as an antioxidant as a fermentation extract of the medicinal materials of Examples 2 to 89 and a reference material, respectively, to prepare a sample at a concentration of 200 μg / ml. Then, the sample and standard DPPH solution were added at the same ratio, stirred well, reacted at 37 ° C for 30 minutes, and absorbance was measured at 520 nm. At this time, ethanol was added instead of the fermented extract of the medicinal product to prepare a control. The free radical scavenging ratio (%) was determined using the following equation (1), and the results are shown in Tables 6 to 8 below.

[Equation 1]

sample PEG increase (pg / well) % Inhibition Control (negative control) - - Arachidonic acid (positive control) 219 - Indomethacin (positive control) 14 93.6 YM badge 195 11.0 MRS badge 183 16.4 BL medium 190 13.2 Gold silver Medicinal self 155 29.2 The yeast fermented extract of Example 2 120 45.2 The fermented extract of lactic acid bacteria of Example 26 116 47.0 The bifidobacterial fermentation extract of Example 50 125 42.9 The fungal fermentation extract of Example 74 119 45.7 Sasayam Medicinal self 136 37.9 The fermented yeast extract of Example 3 105 52.1 The fermented extract of lactic acid bacteria of Example 27 102 53.4 The bifidobacterial fermentation extract of Example 51 110 49.8 The fungal fermentation extract of Example 75 95 56.6 Sophora Medicinal self 139 36.5 The fermented yeast extract of Example 4 112 48.9 The fermented extract of lactic acid bacteria of Example 28 120 45.2 The bifidobacterial fermentation extract of Example 52 108 50.7 The fungal fermentation extract of Example 76 111 49.3 Skirt mushroom Medicinal self 140 36.1 The fermented yeast extract of Example 5 125 42.9 The fermented extract of lactic acid bacteria of Example 29 127 42.0 The bifidobacterial fermentation extract of Example 53 110 49.8 The fungal fermentation extract of Example 77 116 47.0 green tea Medicinal self 141 35.6 The yeast fermented extract of Example 6 127 42.0 The fermented extract of lactic acid bacteria of Example 30 119 45.7 The bifidobacterial fermentation extract of Example 54 115 47.5 The fungal fermentation extract of Example 78 112 48.9 Situation mushroom Medicinal self 137 37.4 The yeast fermented extract of Example 7 102 53.4 The fermented extract of lactic acid bacteria of Example 31 99 54.8 The bifidobacterial fermentation extract of Example 55 104 52.5 The fungal fermentation extract of Example 79 107 51.1 Scissors Medicinal self 151 31.1 The yeast fermented extract of Example 8 130 40.6 The fermented extract of lactic acid bacteria of Example 32 122 44.3 The bifidobacterial fermentation extract of Example 56 121 44.7 The fungal fermentation extract of Example 80 118 46.1 Low thread Medicinal self 142 35.2 Example 9 Yeast Fermentation Extract 127 42.0 The fermented extract of lactic acid bacteria of Example 33 112 48.9 The bifidobacterial fermentation extract of Example 57 121 44.7 Example 81 Mold fungus extract 120 45.2

sample PEG increase (pg / well) % Inhibition goldthread Medicinal self 145 33.8 The yeast fermented extract of Example 10 127 42.0 The fermented extract of lactic acid bacteria of Example 34 117 46.6 The bifidobacterial fermentation extract of Example 58 120 45.2 The fungal fermentation extract of Example 82 111 49.3 Changwon Medicinal self 140 36.1 The fermented yeast extract of Example 11 122 44.3 The fermented extract of lactic acid bacteria of Example 35 115 47.5 The bifidobacterial fermentation extract of Example 59 109 50.2 The fungal fermentation extract of Example 83 117 46.6 magnolia Medicinal self 150 31.5 The fermented yeast extract of Example 12 127 42.0 The fermented extract of lactic acid bacteria of Example 36 126 42.5 The bifidobacterial fermentation extract of Example 60 114 47.9 The fungal fermentation extract of Example 84 119 45.7 Moon style Medicinal self 153 30.1 The fermented yeast extract of Example 13 127 42.0 The fermented extract of lactic acid bacteria of Example 37 114 47.9 The bifidobacterial fermentation extract of Example 61 134 38.8 The fungal fermentation extract of Example 85 126 42.5 Bleacher Medicinal self 139 36.5 The fermented yeast extract of Example 14 110 49.8 The fermented extract of lactic acid bacteria of Example 38 109 50.2 The bifidobacterial fermentation extract of Example 62 104 52.5 The fungal fermentation extract of Example 86 113 48.4 Mutual debt Medicinal self 146 33.3 The yeast fermented extract of Example 15 116 47.0 The fermented extract of lactic acid bacteria of Example 39 122 44.3 The bifidobacterial fermentation extract of Example 63 124 43.4 The fungal fermentation extract of Example 87 116 47.0 Saury Medicinal self 139 36.5 The yeast fermentation extract of Example 16 115 47.5 The fermented extract of lactic acid bacteria of Example 40 113 48.4 The bifidobacterial fermentation extract of Example 64 126 42.5 The fungal fermentation extract of Example 88 111 49.3 Embarkation Medicinal self 143 34.7 The yeast fermented extract of Example 17 125 42.9 The fermented extract of lactic acid bacteria of Example 41 127 42.0 The bifidobacterial fermentation extract of Example 65 117 46.6 The fungal fermentation extract of Example 89 121 44.7 Cherry Medicinal self 147 32.9 The yeast fermented extract of Example 18 123 43.8 The fermented extract of lactic acid bacteria of Example 42 110 49.8 The bifidobacterial fermentation extract of Example 66 119 45.7 The fungal fermentation extract of Example 90 122 44.3

sample PEG increase (pg / well) % Inhibition Yugunpi Medicinal self 135 38.4 Example 19 Yeast Fermentation Extract 104 52.5 The fermented extract of lactic acid bacteria of Example 43 109 50.2 The bifidobacterial fermentation extract of Example 67 97 55.7 The fungal fermentation extract of Example 91 103 53.0 Shipment Medicinal self 150 31.5 The yeast fermented extract of Example 20 114 47.9 The lactic acid bacteria fermented extract of Example 44 120 45.2 The bifidobacterial fermentation extract of Example 68 122 44.3 The fungal fermentation extract of Example 92 125 42.9 Ji Medicinal self 147 32.9 The fermented yeast extract of Example 21 120 45.2 The fermented extract of lactic acid bacteria of Example 45 129 41.1 The bifidobacterial fermentation extract of Example 69 114 47.9 The fungal fermentation extract of Example 93 117 46.6 Car electronics Medicinal self 147 32.9 The yeast fermented extract of Example 22 117 46.6 The fermented extract of lactic acid bacteria of Example 46 120 45.2 The bifidobacterial fermentation extract of Example 70 125 42.9 The fungal fermentation extract of Example 94 119 45.7 Window Medicinal self 146 33.3 The fermented yeast extract of Example 23 118 46.1 The fermented extract of lactic acid bacteria of Example 47 119 45.7 The bifidobacterial fermentation extract of Example 71 126 42.5 The fungal fermentation extract of Example 95 120 45.2 Mold Medicinal self 151 31.1 The yeast fermented extract of Example 24 117 46.6 The fermented extract of lactic acid bacteria of Example 48 120 45.2 The bifidobacterial fermentation extract of Example 72 122 44.3 The fungal fermentation extract of Example 96 125 42.9 Hoi Cho Medicinal self 141 35.6 The fermented yeast extract of Example 25 107 51.1 The fermented extract of lactic acid bacteria of Example 49 112 48.9 The bifidobacterial fermentation extract of Example 73 117 46.6 The fungal fermentation extract of Example 97 113 48.4

As shown in Tables 6 to 8, each of the fermented extracts obtained according to Examples 2 to 89 had a free radical scavenging ratio as compared with the respective medicinal materials themselves or the base medium or ascorbic acid used as a known antioxidant All were high.

Example 90 and Comparative Example 1

Using the health fungus fermented extract of Example 68, an ointment for external use on skin was prepared as shown in Table 9 below.

division Example 90 Comparative Example 1 Healthy mold fermentation extract 10 - Diethyl sebacate 8 8 Nonsense 5 5 Polyoxyethylene oleyl ether phosphate 6 6 Sodium benzoate Suitable amount Suitable amount vaseline To 100 To 100

Example 91 and Comparative Example 2

Using the health fungus fermentation extract of Example 68, a cream was prepared according to the prescription of Table 10 below.

division Example 91 Comparative Example 2 Healthy mold fermentation extract 1.0 - Stearic acid 15.0 15.0 Cetanol 1.0 1.0 Potassium hydroxide 0.7 0.7 glycerin 5.0 5.0 Propylene glycol 3.0 3.0 antiseptic Suitable amount Suitable amount incense Suitable amount Suitable amount Purified water To 100 To 100

Example 92 and Comparative Example 3

Using the health fungus fermentation extract of Example 68, a softening longevity was prepared according to the prescription of Table 11 below.

division Example 92 Comparative Example 3 Healthy mold fermentation extract 0.2 - ethanol 10.0 10.0 Polyoxyethylene sorbitan polylauric acid 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 glycerin 5.0 5.0 1,3-butylene glycol 6.0 6.0 incense Suitable amount Suitable amount Pigment Suitable amount Suitable amount Purified water To 100 To 100

Example 93 and Comparative Example 4

Using the health fungus fermented extract of Example 68, a nutritional lotion was prepared according to the prescription of Table 12 below.

division Example 93 Comparative Example 4 Healthy mold fermentation extract 0.1 - Vaseline 2.0 2.0 Sesquioleic acid sorbitan 0.8 0.8 Polyoxyethylene oleylethyl 1.2 1.2 Methyl paraoxybenzoate Suitable amount Suitable amount Propylene glycol 5.0 5.0 ethanol 3.2 3.2 Carboxyvinyl polymer 18.0 18.0 Potassium hydroxide 0.1 0.1 Pigment Suitable amount Suitable amount incense Suitable amount Suitable amount Purified water To 100 To 100

Example 94 and Comparative Example 5

Using the health fungus fermented extract of Example 68, a pack was prepared according to the prescription shown in Table 13 below.

division Example 94 Comparative Example 5 Healthy mold fermentation extract 0.5 - glycerin 5.0 5.0 Propylene glycol 4.0 4.0 Polyvinyl alcohol 15.0 15.0 ethanol 8.0 8.0 Polyoxyethylene oleylethyl 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 incense Suitable amount Suitable amount Pigment Suitable amount Suitable amount Purified water To 100 To 100

Example 95 and Comparative Example 6

Using the health fungus fermentation extract of Example 68, an essence was prepared according to the prescription of Table 14 below.

division Example 95 Comparative Example 6 Healthy mold fermentation extract 5.0 - Propylene glycol 10.0 10.0 glycerin 10.0 10.0 Sodium hyruronate aqueous solution (1%) 5.0 5.0 ethanol 5.0 5.0 Polyoxyethylene hardened castor oil 1.0 1.0 Methyl paraoxybenzoate 0.1 0.1 incense Suitable amount Suitable amount Purified water To 100 To 100

Experimental Example 2. Anti-Aging Effect

The anti-aging effect of the cosmetic composition containing the health fermented extract was verified as follows.

Twenty hairless mice of 15 to 20 weeks of age were used per experiment group as follows. Ultraviolet light was irradiated at 2 mJ / cm 2 dose using an ultraviolet light irradiator (UV simulator, US) on the dorsal area of hairless mice, and this was induced for 2 weeks per week for 4 weeks to induce photoaging of the skin of mice. Thereafter, the cosmetic formulations containing the health fermentation extracts of Examples 90 to 95 were applied to one side of the hairless mouse twice, twice a day for two weeks, and on the other side, a healthy fermented extract The cosmetic compositions of Comparative Examples 1 to 6, which were not contained, were applied. At this time, the packs of Example 124 and Comparative Example 5 were peeled off after 15 minutes. After two weeks, the effect of aging was compared with the naked eye, and the results were evaluated in three stages: marked effect, effect, and no difference. The results are shown in Table 15.

division Distinct effect (n.) Effective (persons) No difference (persons) Example 90 6 7 7 Example 91 4 8 8 Example 92 3 9 8 Example 93 4 8 8 Example 94 3 8 9 Example 95 4 7 9

As shown in Table 15, the cosmetic compositions containing the health fermented extract prepared according to Examples 90 to 95 exhibited an anti-aging effect against at least 10 of 20 subjects, and in particular, a large amount of health fermented extract In Example 90 and Comparative Example 1 in which the health fermentation extract was not applied showed an anti-aging effect of 70%, and no side effects were observed in the skin. Thus, the health fermented extract was found to have a safe and highly effective antioxidant function It was found that it was an anti-aging cosmetic product.

The cosmetic composition having an antioxidative activity according to the present invention is excellent in skin-aging and antioxidative effect and persistence of effect, while being safe for skin.

Claims (11)

Healthy, Seomoktae, Pumpkin, Deodok, Doin, Seokchangpo, Truffle, Chrysanthemum, Oggipi, Jejong, Yongjongyong, As an active ingredient, a fermented extract of a medicinal substance selected from the group consisting of at least one compound selected from the group consisting of The method according to claim 1, Wherein the fermentation extract is obtained by culturing yeast, lactic acid bacteria, or bifidobacteria with a medicament. 3. The method of claim 2, Wherein the fermentation extract is added to the basic medium of yeast, lactic acid bacteria or bifidobacteria in an amount of 1 to 20% by weight. 3. The method of claim 2, Wherein the yeast is selected from the group consisting of Saccharomyces cerevisiae , Schizosaccharomyces ellipsoids and Saccharomyces boulardii . 3. The method of claim 2, Wherein the lactic acid bacteria are selected from the group consisting of Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus reuteri and Lactobacillus confusus, Lactobacillus fermentum and Lactobacillus brevis . 3. The method of claim 2, Wherein the bifidobacteria are selected from the group consisting of Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum and Bifidobacterium infantis . The method according to claim 1, Wherein the fermented extract is obtained by inoculating fungi into a medicinal material and solid fermentation. 8. The method of claim 7, Wherein the fungus is selected from the group consisting of Inonnotus-obliquus, Cordyceps sinensis, Paecilomyces japonica , phellinus linteus and Ganoderma lucidum. ≪ / RTI > The method according to claim 1, Wherein the medicinal fermentation extract is contained in an amount of 0.0001 to 10% by weight in the cosmetic composition. The method according to claim 1, Wherein the cosmetic composition is a formulation of an ointment for external use, a softening agent, a nutritional lotion, a nutritional cream, a massage cream, an essence, a pack, an emulsion or an oil gel. The method according to claim 1, The cosmetic composition according to claim 1, further comprising an additive selected from the group consisting of oil, water, surfactant, humectant, lower alcohol, thickener, chelating agent, pigment, preservative, Composition.