KR101375137B1 - A cosmetic composition comprising fermented extract of Scutellaria baicalensis using Leatiporus sulphureus var. miniatus for skin moisturizing - Google Patents

Abstract

The present invention relates to a cosmetic composition containing fermented red myrtle mycelium mycelium of the golden extract, wherein the fermented red mycelium mycelium mycelium fermented product of the golden extract is effectively converted into a glycoside Baikalin non-glycoside Baikallein By containing a high concentration of the bikallein can be effectively used as a high functional cosmetic composition for skin improvement by excellent antioxidant and skin moisturizing effect.

Description

A cosmetic composition comprising fermented extract of Scutellaria baicalensis using Leatiporus sulphureus var. miniatus for skin moisturizing}

The present invention relates to a cosmetic composition for moisturizing skin containing red mycelial mycelium fermented product of golden extract.

In the human body, the skin acts as a protective film to protect the living body from the external environment. It prevents the loss of biological components such as water or electrolytes and prevents harmful substances from entering the body. .

The skin is divided into the skin layer, the dermal layer and the subcutaneous fat layer. The epidermal layer is composed of keratinocytes and melanocytes. Since the outermost keratinocyte layers of skin are in direct contact with the external environment, they require strong resistance to physical, chemical, or permeation of substances, and prevent the loss of moisture from the body to the outside. At the same time, it should maintain flexibility by retaining adequate moisture on its own.

Generally, the moisture content of the skin is about 70% in the dermal layer, but decreases to the skin layer, which is about 10 to 30% in the keratin layer. The water supplied from the dermis layer mainly migrates to the upper part of the stratum corneum by passive diffusion and eventually to the outside. This is called Trans Epidermal Water Loss (TEWL), and it is known that it is the sebaceous component and the epidermal lipid which are the lipid membrane components of the keratinocyte layer to maintain the appropriate level of transdermal moisture loss. In addition, the keratinocyte layer is known to play an important role in moisturizing the skin due to the presence of a substance having a hydrophilic water retention capability called a natural moisturizing factor (NMF). When the moisture of the normal keratinocyte layer is maintained about 10 to 30%, the skin is smooth and soft, and the body protects normally.

However, when the moisture content of the keratinocyte layer is less than 10%, the skin becomes rough and the body loses its protective function, causing aging. For example, in the case of dry skin, the aggregation force of keratinocytes is weakened, and scaling phenomenon in which keratinocytes are peeled off from the surface of the skin, such as scale fragments, occurs. Thus, the drying of the skin is the moisture content of the keratinocyte layer. This is because it is less than normal skin. In addition, even healthy skin may suffer from a lack of moisture due to harsh external environment, ie, wind, cold weather, sunlight, face wash, shaving, and so on, may cause a bad skin condition.

Therefore, it is very important to maintain the moisture content of the keratinocyte layer of the skin properly. To this end, cosmetics such as sebum-like components, NMF components, or moisturizers such as polyols were added and used. For example, glycerin, solitol, and the like having three or more hydroxyl groups (OH groups) as water-soluble polyols exhibit excellent moisturizing power, but are highly sticky, causing unpleasant feelings when used, and having two hydroxyl groups, propylene glycol and 1,3-butylene. Glycol and other side effects may occur. In addition, other natural moisturizing factors, such as sodium pyrrolidone carboxylate (PCA-Na), sodium lactate, urea, etc., are highly electrolytic and thus impair the emulsion stability of cosmetics, and also moisturize amino acids, collagen and elastin. There is a capacity, but the moisturizing ability is limited. The ability to retain moisture in the stratum corneum is controlled by the natural moisturizing factor (NMF), which consists of sebum, amino acids, lactic acid, urea and inorganic salts. There is a need for the development of high materials.

Golden ( Scutellaria baicalensis ) is a perennial herb of dicotyledon and currency plant Lamiaceae. It is native to the East Asian continent and mainly distributed in Korea, China and Siberia. It's called decaying grass, togeumum, and perfumed plants, and the roots of peeled skin are used as herbal medicine. In the oriental medicine, roots are used as antipyretic, diuretic, branch, yedam and anti-inflammatory agents. The main active ingredients of gold are flavonoid glycosides such as baicalin and wogonoside, and non-glycoins baicalein and wogonin are known to be present in trace amounts. It is known that the non-glycoside forms have higher physiological activity than glycosides. Recently, studies have been conducted on the effects of the hepatocyte protection, liver cirrhosis suppression, gastric damage inhibition, gastric acid inhibition, Helicobacter pylori inhibition, and joint pain relief effects of the active ingredients. On the other hand, Baikalrin, the main component of gold, is relatively slow in absorption and narrow in pharmacological application, whereas Baikallein has been reported to have faster absorption in the body and a greater pharmacological value than Baikallin. have. However, it is known that it is difficult to obtain bikallein from gold by using a direct extraction method because the bikallein is present in a very small amount (containing about 0.2 to 0.5%) in gold.

The red duck mushroom ( Leatiporus sulphureus var.miniatus) belongs to the fungus fungi family, and is native to Baekdusan, Wolchulsan, Jirisan, and Balwangsan, and is distributed throughout the tropical regions of Japan and Asia. From spring to summer, it is a single-year-old heartwood of coniferous trees, dead trees, and regenerated stumps. Red duck mushrooms have long been reported to be effective in controlling function, improving health, and anti-disease, but the exact pharmacological effects are unknown. Red duck mushroom fruiting bodies include N-methylated tyramine derivatives, polysaccharides, lanostane triterpenoids, retipoic acid and various other mixtures. Recently, red duck fungus has been reported to have anticancer and insulin secretory effects. The anticancer effect of red duck fungus inhibits cancer metastasis and enhances immunity. In addition, red duck fungus mycelium extracellular polysaccharide Is known to promote skin cell growth factor, wound healing effect and skin moisturizing effect. Red duck mushrooms are also known to produce large amounts of functional enzymes, including glucosidase.

The inventors of the present invention, while studying the possibility of applying the cosmetics of various natural products, the red thigh mushroom mycelium fermented product of the golden extract shows an excellent antioxidant effect, by confirming that the skin moisturizing effect is excellent and can be used as a high-functional cosmetic composition, I could complete it.

On the other hand, Korean Patent No. 1081913 discloses a cosmetic composition containing a fermented product obtained by fermenting a herbal extract containing some of the gold as a constituent with leaves. In addition, Korean Patent No. 910040 and Korean Patent No. 966835 disclose a cosmetic composition containing a fermented product prepared by fermenting a herbal extract containing golden as a component of yeast, lactic acid bacteria, bifidus, Bacillus, etc. Is disclosed. In addition, Korean Patent Application Publication No. 2011-0108687 and Korean Patent Publication No. 2011-0108686 disclose a cosmetic composition containing a fermented product prepared by treating a herbal extract containing golden extract as part of a glycosidase. have. However, the fermented products of the golden extract may be said to have a completely different technical configuration from the fermented product of the golden extract of the present invention, which fermented the golden extract using red duck mushroom mycelium.

Korean Registered Patent No. 1081913 (External Skin Composition Containing Fermented Herbal Extract, Registered on Nov. 3, 2011) Korea Patent Registration No. 910040 (Cosmetic composition having antioxidant activity, registered on July 23, 2009) Korean Registered Patent No. 966835 (Contains extracts of plant mixtures consisting of licorice, horseback riding, sewage, black sesame seeds, mushrooms, donkeys, lettuce and white currants, white ginseng, red ginseng, and gold that have skin protection against ultraviolet rays and whitening and anti-wrinkle effects Cosmetic composition and preparation method thereof, registered on June 22, 2010) Korean Unexamined Patent Publication No. 2011-0108687 (Whitening agent comprising natural raw material extract and cosmetic composition comprising the same, 2010.03.29) Korean Unexamined Patent Publication No. 2011-0108686 (Antioxidant containing natural raw material extract and feed, food, or cosmetic composition comprising the same, published on March 29, 2010)

It is an object of the present invention to provide a skin moisturizing cosmetic composition containing a red duck mushroom mycelium fermented product of golden extract.

The present invention relates to a cosmetic composition for moisturizing skin containing red mycelial mycelium fermented product of golden extract.

The golden extract may be an extract prepared by extracting gold with water, lower alcohol having 1 to 4 carbon atoms, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, hexane or a mixed extractant thereof.

The golden extract, after adding 1 to 20 times the extraction solvent of the weight of gold to gold, it can be prepared by extracting for 5 minutes to 10 hours at 40 ~ 100 ℃.

Fermented red mycelium mycelium mycelium mycelium of the golden extract may be fermented by adding 1 ~ 35% by weight of the golden extract on the basis of the medium to the red green mushroom mycelium culture medium.

Red duck mushroom mycelium fermented product of the golden extract may contain 0.001 to 30.0% by weight in the total cosmetic composition.

Therefore, preferably the present invention can provide a cosmetic containing a cosmetic composition containing the red myrtle mycelium fermented product.

Hereinafter, the present invention will be described in detail.

The golden extract may be an extract prepared from water, a lower alcohol having 1 to 4 carbon atoms, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, hexane, or a mixed solvent thereof, and the lower 1 to 4 carbon atoms. The alcohol can be selected from ethanol, methanol, propanol, isopropanol and butanol. In addition, the butanol may be 100% butanol or saturated butanol which is 75-85% (v / v) butanol aqueous solution. The golden extract is added to the extract solvent of 1 to 20 times the weight of the gold to extract the extract for 5 minutes to 10 hours at 40 ~ 100 ℃, and extracting the filtrate by removing the solids from the extract, The filtrate may be an extract prepared in the form of a dried product by drying under reduced pressure concentration or freeze drying, spray drying, cold air drying, hot air drying and the like.

When preparing the golden extract, after the extraction solvent is added, it is possible to increase the extraction effect of the active ingredient by high pressure extraction using ultra high pressure technology (Ultra high pressure). Preferably, after adding the extraction solvent to the gold, it can be extracted at a pressure of 100 ~ 3000MPa, more preferably can be extracted at a pressure of 500 ~ 1000MPa. In addition, the high-pressure extraction may be preferably performed by lowering the pH of the water inside the ultrahigh pressure to 1-6. For the high pressure extraction, an ultra high pressure processor may be used. On the other hand, the extraction time at high pressure extraction may be preferably 5 to 60 minutes.

Fermented red mycelium mycelium mycelium mycelium of the golden extract, may be prepared by fermentation by adding 1 to 35% by weight of the golden extract (based on dry matter) to the medium for red red mushroom mycelium culture, The red duck mushroom mycelium culture medium is 1 to 20 parts by weight of glucose (glucose), 0.1 to 10 parts by weight of yeast extract, 0.01 to 1 parts by weight of polypeptone (polypeptone), MgSO _It may be a medium containing 0.01-1 part by weight of ₄ · 7H ₂ O and 0.01-1 part by weight of K ₂ HPO ₄ .

At this time, the golden extract can be fermented by adding to the red mushroom fungus mycelium culture medium in the aqueous solution of 1 ~ 70% (w / v). Preferably, the water content of the red duck mushroom mycelium culture medium can be reduced by the amount of the aqueous solution of 1 to 70% by weight of the golden extract is added. For example, when preparing 100 ml of red mycelium mycelium culture medium, only 50% of water is added, and the remaining 50% of water is added to the 50% aqueous solution of the golden extract. After addition to the culture medium can be fermented.

Fermented red mycelium mycelium mycelium of the golden extract, fermented fermented product containing 0.001 ~ 10% by weight of the red mycelium mycelium mycelium or a culture solution precultured based on the red mycelium mushroom mycelium culture medium have. At this time, the pure mycelium may be contained 0.00001 ~ 2% by weight based on the red duck mushroom mycelium culture medium.

Red duck mushroom mycelium fermented product of the golden extract may be contained in the total cosmetic composition 0.001 ~ 50.0% by weight, preferably 0.001 ~ 30.0% by weight.

Therefore, the present invention provides a method for preparing a red mycelium mushroom mycelium fermented product of the golden extract. Preferably,

(Step 1) preparing a golden extract;

(2 step) fermenting the golden extract using red myrtle ( Leatiporus sulphureus var. Miniatus) mycelium; And

(3 step) removing the red myrtle mushroom mycelium from the fermentation; can be prepared, and then, concentrated under reduced pressure or lyophilized can be prepared in a concentrate or powder state.

The golden extract of the first step may be used in a concentrated state or in a dried state, and may be used after being concentrated or dried and suspended in water to prepare an aqueous solution.

When fermenting the golden extract of the second step, on the basis of when the golden extract is in the dry state, the red thigh mushroom mycelium culture medium can be added to contain 1 to 35% by weight of the total gold extract can be fermented have.

In addition, the cosmetic composition may contain other ingredients that can increase the skin moisturizing effect of the present invention in addition to the red myrtle mycelium fermented product of the golden extract of the present invention.

On the other hand, the red duck mushroom mycelium fermented product of the golden extract may be used in cosmetics in a liquid state immediately after fermentation, it may be used in a dried state dried by a conventional drying method. The drying method may be a method such as reduced pressure drying, freeze drying, spray drying, cold air drying.

Cosmetic compositions according to the present invention comprise auxiliaries commonly used in the cosmetic field, such as hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic active agents, preservatives, antioxidants, solvents, fragrances, fillers, blockers, pigments, odorants or dyes. It may contain. The amount of the adjuvant is an amount conventionally used in the art, such as 0.001 to 70% by weight, preferably 0.001 to 50% by weight based on the total weight of the cosmetic composition. However, in any case, the adjuvant and its proportion will be selected so as not to adversely affect the desirable properties of the cosmetic composition according to the present invention.

On the other hand, the cosmetic composition of the present invention is preferably a lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) and water-in-oil (W / O) type emulsion, ointment cosmetic formulation, oil-in-water and Water-in-oil makeup base, foundation, skin cover, lipstick, lip gloss, face powder, two-way cake, eye shadow, teak color and eyebrow pencils can be prepared in any one of the formulations selected from the cosmetic formulation. It may also be optionally prepared in the form of an aerosol and applied to the skin, may be in the form of a solid such as a stick, or may be made into a skin care product and / or a makeup product.

The present invention relates to a cosmetic composition containing fermented red myrtle mycelium mycelium of the golden extract, wherein the fermented red mycelium mycelium mycelium fermented product of the golden extract is effectively converted into a glycoside Baikalin non-glycoside Baikallein By containing a high concentration of the bikallein can be effectively used as a high functional cosmetic composition for skin improvement by excellent antioxidant and skin moisturizing effect.

1 is a red duck mushroom mycelium fermentation product of the golden extract of Example 3 of the present invention, the golden extract before fermentation of Example 1, Baikallin and Baikallein of the red duck mushroom mycelium fermentation of the golden extract of Comparative Examples 1 to 3 It is a graph showing the concentration.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein but may be embodied in other forms. Rather, the intention is to provide an exhaustive, complete, and complete disclosure of the principles of the invention to those skilled in the art.

Example 1 Preparation of Golden Extract

Example 1-1

The dried commercial gold was selected as a sample, thoroughly washed and then ground to make fine. 100 g / l was added to the final 70% (v / v) ethanol aqueous solution, extracted for 60 minutes at a pressure of 1000 MPa and a temperature of 50 ° C. using an ultrahigh pressure processor, and then cooled to room temperature. The pH was lowered to 3.0 and treated. A portion of this extract was filtered through whatman # 10 filter paper. The filtrate thus obtained was again filtered using a filter of 0.25 μM, concentrated under reduced pressure and lyophilized to prepare a dried product.

Examples 1-2

Prepared in the same manner as in Example 1-1, but pulverized gold was added to 70% (v / v) ethanol aqueous solution to 100g / ℓ extracted for 60 minutes at 80 ℃ and cooled to room temperature, whatman # 10 Filter by filter paper. The filtrate thus obtained was again filtered using a filter of 0.25 μM, concentrated under reduced pressure and lyophilized to prepare a dried product.

Example 1-3

Prepared in the same manner as in Example 1-1, but pulverized gold was added to purified water to 100g / l, extracted for 60 minutes at 100 ℃, cooled to room temperature, and filtered with Whatman # 10 filter paper. The filtrate thus obtained was finally filtered using a filter of 0.25 μM and lyophilized to prepare a dried product.

Example 2 Cultivation of Red Duck Mushroom Mycelium

The red mycelium mushroom mycelium (CS0218 KFCC 11494P) was incubated for 7 days at 25 ° C. in a medium of PDA (potato dextrose agar), and then refrigerated at 4 ° C. and passaged at intervals of about 2 months.

The spawn culture for fermenting the golden extract was 50 g of water, 1.5 g of glucose, 0.3 g of yeast extract, 0.1 g of polypeptone, 0.025 g of MgSO ₄ · 7H ₂ O, K ₂ HPO ₄ was mixed to 0.025g to inoculate mycelium in a sterile prepared medium, and then cultured at 25 ° C., 150rpm for 7 days.

Example 3 Preparation of Red Duck Mushroom Mycelia Fermentation of Golden Extract

Fermented red mycelium mycelium mycelium fermented product of the golden extract of the present invention was prepared by adding golden extract to the culture medium when cultivating red mycelium mycelium (50% less purified water, the remaining 50% To an aqueous solution of the golden extract). To this end, 25 ml of 50% (w / v) aqueous solution of the golden extract of Example 1 (see Table 1), 25 ml of water, 1.5 g of glucose, 0.3 g of yeast extract, 0.1 g of polypeptone, MgSO ₄ · 7H ₂ 0.025 g O, 0.025 g K ₂ HPO ₄ were mixed and sterilized. After adding the red duck mushroom mycelium spawn culture of Example 2 to the medium composition to 10% by weight (containing 0.1 g as pure mycelium), the golden extract was fermented by incubating at 25 ° C. and 150 rpm for 7 days. After the fermentation was completed, the filtrate obtained by removing all the red mycelium mycelium mycelium by centrifugation was used as a fermented red mycelium mycelium mycelium.

Condition Fermentation Example 3-1 Fermented product obtained by fermenting the extract of Example 1-1 Example 3-2 Fermented product obtained by fermenting the extract of Example 1-2 Example 3-3 Fermented product obtained by fermenting extract of Example 1-3

Example  4: of golden extract Red duck mushroom  mycelium The fermented product  Preparation of containing cream

A cream comprising the composition of the present invention was prepared. The composition is as showing in Table 2. To this end, the 'b' phase (fermented products of Example 3-1 to 3-3, liquid phase) recorded in Table 2 was heated to 70 ° C., followed by preliminary emulsification by adding a 'ga' phase. Emulsified homogeneously with and then cooled slowly to prepare a cream.

Condition weight% Example 4-1 Example 4-2 Example 4-3



end Stearyl alcohol 8 8 8 Stearic acid 2 2 2 Stearic acid cholesterol 2 2 2 Squalane 4 4 4 2-octyldodecyl alcohol 6 6 6 Polyoxyethylene (25 mol addition) alcohol ester
3
3
3 Glyceryl monostearate
Aster
2
2
2
I Fermentation of Example 3-1 5 - - Fermentation of Example 3-2 - 5 - Fermentation of Example 3-3 5 Purified water to 100 to 100 to 100

Comparative Example  1.Lactobacillus of Golden Extract Fermentation product  Produce

Examples 3-1 and Lactobacillus ash FIG filler's (Lactobacillus fermentation, but in the Golden extract by the same method, known to produce a non-red deokdari mushroom mycelium extract the aqueous solution of gold Let β- glucosidase the (β-glucosidase) acidophilus ATCC 9224) was added to the culture medium. To this end, MRS medium was used as a basal medium of Lactobacillus ashidophilus. In preparing MRS medium, 50% less purified water was added, and 50% aqueous solution (w / v) of the golden extract of Example 1-1 was added to the remaining 50%. Lactobacillus spawn culture was inoculated to 10 ⁷ cells / ㎖ and incubated for 48 hours at 37 ℃ fermentation. After the fermentation was completed, the filtrate obtained by centrifuging the culture solution to remove the cells was prepared as lactic acid bacteria fermentation product of the golden extract.

Comparative Example 2. Preparation of leaf mushroom fermented product of golden extract

The golden extract was fermented in the same manner as in Example 3-1, but was fermented using the leaf mycelium mycelium instead of the red mycelium mycelium. The leaf mushroom was used after mycelial growth using those purchased from the traditional market.

Comparative Example 3. Enzyme Fermentation of Golden Extract

Ferment the golden extract in the same manner as in Example 3-1, adjust the pH of the aqueous solution of the golden extract to 5.5, and then add 0.02 g of glycosidase (Fungamyl, Sebamyl, and Clariseb; Special Enzyme, USA) to 3 After reacting for an hour, the temperature of the extract was raised to about 80 ° C. to inactivate the enzyme, which was prepared as an enzyme fermentation of the golden extract (medium composition was added in the same way).

Comparative Example 4 Preparation of a Cream Containing Red Thigh Mushroom Mycelium Fermented Product of Golden Extract

To prepare a cream in the same manner as in Example 4, a part of the condition ('na' phase) under the conditions of the following Table 3 to prepare a comparative cream.

Condition weight% Comparative Example 4-1 Comparative Example 4-2 Comparative Example 4-3 Comparative Example 4-4 Comparative Example 4-5 Comparative Example 4-6 Comparative Example 4-7




end Stearyl alcohol 8 8 8 8 8 8 8 Stearic acid 2 2 2 2 2 2 2 Stearic acid
cholesterol
2
2
2
2
2
2
2 Squalane 4 4 4 4 4 4 4 2-octyldodecyl alcohol 6 6 6 6 6 6 6 Polyoxyethylene
(With 25 moles)
Alcohol ester
3
3
3
3
3
3
3 Glyceryl monostearate Aster
2
2
2
2
2
2
2





I Example 1-1
extract
5
-
-
-
-
-
- Examples 1-2
extract
-
5
-
-
-
-
- Examples 1-3
extract
-
-
5
-
-
-
- Comparative Example 1
Fermentation product
-
-
-
5
-
-
- Comparative Example 2
Fermentation product
-
-
-
-
5
-
- Of Comparative Example 3
Fermentation product
-
-
-
-
-
5
- Na-hyaluronic acid salt - - - - - - 5 Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100

Test Example  One. Baikalin  And Baikalein  Check concentration

The concentration of Baikallin and Baikallein of red duck mushroom mycelium fermentation of the golden extract of the present invention was confirmed by HPLC and the results are shown in FIG. HPLC experimental conditions are as follows.

Instrument-Waters (Waters e1225 module, Waters 2998 PDA)

Column-Waters Capcellpak C18 (5 μm, 4.6 × 250 mm)

Flow rate-1 ml / min

Wavelength-277nm

Mobile phase-acetonitrile (%): 0.125% aqueous formic acid solution (%) = 10:90 (0 minutes), 65:35 (45 minutes), 10:90 (70 minutes)

1, it was confirmed that the content of Baikallein in the red myrtle mycelium fermented product of the golden extract of the present invention of Example 3 significantly increased. On the other hand, since the fermentation product of the present invention appears to have a better efficiency of conversion of Baikallein than the fermentation product of the golden extract prepared using the purified enzyme, considering the cost used to purify the enzyme, red mushroom mycelium Fermentation of the golden extract was expected to be useful in terms of cost.

Test Example 2. Confirmation of Antioxidant Effect

The antioxidant effect of the red thigh fungus mycelium fermentation of the golden extract of the present invention was measured using the NBT (Nitro Blue Tetrazolium) method. In the NBT method, the active oxygen scavenging rate is measured by measuring the blue color produced by the reaction of reactive oxygen produced by xanthine and xanthine oxidase with NBT (Nitro Blue Tetrazolium) at a wavelength of 560 nm. At this time, each fermented product was lyophilized and then diluted and treated to the concentration shown in Table 4.

The measuring method is as follows.

0.05M Na ₂ CO ₃ ----------------------- 2.4ml

2.3mM xanthine solution -------------------- 0.1ml

3.3mM EDTA Solution ---------------------- 0.1ml

4.BSA solution --------------------------- 0.1ml

5.0.72mM NBT solution -------------------- 0.1ml

6.Xanthine Oxidase Solution ------------------ 0.1ml

7.6 mM CuCl ₂ solution ---------------------- 0.1 ml

① 1,2,3,4,5 is mixed and 0.1ml (sample) of golden extract or fermented product under each condition is added and left at 25 ° C for 10 minutes.

② Add 6 and stir quickly and react for 20 minutes at 25 ℃.

③ After that, add 7 to stop the reaction and measure the absorbance (St) at 560nm.

④ In the blank test, measure the absorbance (Bt) by using distilled water instead of the sample solution as above.

⑤ The blank of the sample solution is measured in the same manner using distilled water instead of 6 to measure the absorbance (Bo).

The result of the effect was calculated by the following Equation 1, the results are shown in Table 4.

Equation 1

% Inhibition = [1- (St-So) / (Bt-Bo)] × 100

St: Absorbance at 560 nm after enzyme (xanthine oxidase) reaction of sample solution

So: absorbance at 560 nm before reaction without enzyme in sample solution

Bt: Absorbance at 560 nm after enzyme reaction of blank test solution

Bo: absorbance at 560 nm before reaction without enzyme in blank test solution

Name of sample Sample Processing Concentration (% [w / v]) Antioxidative effect(%) Fermentation of Example 3-1 0.01 98 Fermentation of Example 3-2 0.01 79 Fermentation of Example 3-3 0.01 77 Extract of Example 1-1 0.01 12 Extract of Example 1-2 0.01 18 Extract of Example 1-3 0.01 15 Fermented product of Comparative Example 1 0.01 36 Fermented product of Comparative Example 2 0.01 33 Fermentation product of Comparative Example 3 0.01 32 Retinol 0.1 90 BHT 0.1 88

As a result, as shown in Table 4, it was confirmed that all of the compositions of Example 3, the red duck mushroom mycelium fermentation of the golden extract, excellent antioxidant effect.

Test Example 3 Experiment for Measuring Antioxidant Effect Using DPPH Method

In order to measure the antioxidant effect by another method, antioxidant activity was measured using the DPPH method. The DPPH method is a method of measuring the antioxidant activity by reducing power by using a free group called 2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl free radical (DPPH). The free radical scavenging rate is measured at a wavelength of 560 nm by comparing the decrease with the absorbance of the blank. At this time, each fermented product was lyophilized and then diluted and treated to the concentration of Table 5.

The measuring method is as follows.

① Add 0.15 ml of the sample solution to 0.15 ml of 0.1 mM DPPH (Aldrich Chem. Co., MW = 618.76) solution in a 96-well plate, stir rapidly, and incubate for 10 minutes at 25 ° C.

(2) Then measure the absorbance (St) at 560nm.

③ In the blank test, measure the absorbance (Bt) by using distilled water instead of the sample solution as above.

④ Again, the blank of the sample solution was operated in the same manner using methanol instead of the 0.1 mM DPPH solution to measure the absorbance (Bo).

The result of the effect was calculated by Equation 2, the results are shown in Table 5.

Equation 2

% Inhibition = [1- (St-So) / (Bt-Bo)] × 100

St: absorbance at 560 nm after free radical scavenging of the sample solution

So: the absorbance at 560 nm before the reaction in the absence of free radicals in the sample solution

Bt: absorbance at 560 nm after free radical scavenging of the blank test solution

Bo: absorbance at 560 nm before the reaction in the absence of the free radical of the blank test solution

Name of sample Sample processing concentration (%) Antioxidative effect (%) Fermentation of Example 3-1 0.01 93 Fermentation of Example 3-2 0.01 72 Fermentation of Example 3-3 0.01 77 Extract of Example 1-1 0.01 12 Extract of Example 1-2 0.01 13 Extract of Example 1-3 0.01 18 Fermented product of Comparative Example 1 0.01 38 Fermented product of Comparative Example 2 0.01 32 Fermentation product of Comparative Example 3 0.01 30 Green tea extract 0.01 40 Vitamin E 0.01 37

Referring to Table 5, as shown in Test Example 2, the composition of Example 3 had the best antioxidant effect, which was confirmed to be superior to green tea extract and vitamin E.

Experimental Example 4 Experiment for Measuring Skin Moisturizing Effect

The skin moisturizing effect was evaluated using the creams of Example 4 and Comparative Example 4 prepared using the red duck mushroom mycelium fermented product of the golden extract of the present invention. Twenty female test subjects in their 20s and 40s were asked to apply each cream sample to their right and left face and to apply 90 minutes of moisturizing ability to Cornoemeter (CM810, Courage and △ Hydration (humidity increase) was evaluated by Khazaka Electronic Co., Germany).

cream Corneometer (△ Hydration, g / m ² / h) Example 4-1 18 Example 4-2 19 Example 4-3 20 Comparative Example 4-1 4 Comparative Example 4-2 3 Comparative Example 4-3 5 Comparative Example 4-4 7 Comparative Example 4-5 8 Comparative Example 4-6 9 Comparative Example 4-7 7

As shown in Table 6, it can be seen that the moisturizing effect is excellent in the facial skin of the experimenter who applied the cream containing fermented red myrtle mycelium of golden extract.

Test Example 5 Measurement of Transdermal Moisture Loss

The transdermal moisture loss (TEWL: Trans-Epidemal Water Loss) was obtained by using the cream of Example 4 and Comparative Example 4 prepared using the red thigh mushroom mycelium fermented product of the golden extract of the present invention. Co., Germany).

Twenty female experimenters in their 20s and 40s each group per 1 hour, 2 hours, 4 hours, and 6 hours per day before and after application of each sample on the right and left face and 1 cm below 3 cm to the right Moisture loss amount was measured three times using Tewameterr (TM300, Courage and Khazaka Electronic Co., Germany) and the average value was used to evaluate transdermal moisture loss using the average value, which is shown in Table 7 below (the smaller the transdermal moisture loss number, the more effective). Is excellent).

cream Transdermal moisture loss (moisture evaporates from 1㎠ of skin per unit time, g) 1 hours 2 hours 4 hours 6 hours Example 4-1 11.6 10.2 6.1 3.7 Example 4-2 12.8 11.2 7.1 5.6 Example 4-3 12.4 10.8 8.3 6.3 Comparative Example 4-1 18.4 17.1 16.7 14.6 Comparative Example 4-2 17.8 15.2 14.8 14.2 Comparative Example 4-3 18.4 16.8 15.4 13.4 Comparative Example 4-4 17.2 15.8 13.2 11.8 Comparative Example 4-5 18.2 16.6 14.8 11.6 Comparative Example 4-6 16.8 15.4 14.4 12.4 Comparative Example 4-7 18.8 17.2 15.2 14.4

As a result of measuring the amount of transdermal moisture loss as shown in Table 7, the amount of transdermal moisture loss in the facial skin of the experimenter who applied the cream containing the red thigh mushroom mycelium fermentation product of the golden extracts of Examples 4-1 to 4-3 It can be seen that as the flow decreases, and in particular, the amount of transdermal moisture loss was significantly decreased in the experimenter who applied the cream containing the ultra high pressure golden extract fermented with red duck mushroom (Example 4-1).

Claims (6)

A skin moisturizing cosmetic composition comprising a red duck mushroom mycelium fermented product of golden extract. The method of claim 1,
The golden extract is moisturizing skin, characterized in that the extract is prepared by extracting gold with water, lower alcohol having 1 to 4 carbon atoms, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, hexane or a mixed extractant thereof Cosmetic composition for. The method of claim 1,
The golden extract is a skin moisturizing cosmetic composition, characterized in that after the addition of 1 to 20 times the extraction solvent of the golden weight to gold, extracted for 5 minutes to 10 hours at 40 ~ 100 ℃. The method of claim 1,
The red duck mushroom mycelium fermented product of the golden extract is a skin moisturizing cosmetic composition, characterized in that fermented by adding 1 ~ 35% by weight of the golden extract to the medium for red duck mushroom mycelium culture. The method of claim 1,
Red thigh mushroom mycelium fermented product of the golden extract is a moisturizing cosmetic composition, characterized in that the total cosmetic composition contains 0.001 ~ 30.0% by weight. A cosmetic comprising a composition as claimed in any one of claims 1 to 5.

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