KR20180093465A - Cosmetic composition comprising fermented extract of Scutellaria baicalensis using Leatiporus sulphureus for inhibiting skin damage by ultraviolet - Google Patents

Abstract

The present invention relates to a cosmetic composition for inhibiting skin damage caused by ultraviolet rays containing fermented mycelium of Leatiporus sulphureus var. miniatus of a Scutellaria baicalensis extract. The fermented mycelium of Leatiporus sulphureus var. miniatus of the Scutellaria baicalensis extract is confirmed to inhibit the thickening of the skin during the development of the skin damage induced by ultraviolet irradiation. Therefore, a cosmetic composition having an excellent skin damage inhibiting effect is expected to be developed by using the fermented mycelium of Leatiporus sulphureus var. miniatus of the Scutellaria baicalensis extract.

Description

Technical Field [0001] The present invention relates to a cosmetic composition for inhibiting skin damage caused by ultraviolet rays containing a fermented product of reddish brown mushroom mycelium of golden extract,

The present invention relates to a cosmetic composition for inhibiting skin damage caused by ultraviolet rays containing a fermented product of reddissume mushroom mycelium of a golden extract.

There are two main causes of skin aging. The first is natural aging (intrinsic aging) that progresses over time, and the second is exogenous aging due to the external environment and skin damage caused by stimulation. The phenomenon of aging by ultraviolet rays due to extrinsic aging is called photoaging phenomenon (Lee J.N., et al., 2006).

Natural aging is characterized by a decrease in the structure and function of the skin with age, dryness of the skin, fine lines, and disappearance of elasticity. Histologically, epidermal atrophy, epidermal- Flatness of elastin and accumulation of elastin abnormally occur. Photo-aging is caused by ultraviolet (ultraviolet) skin wrinkles formed early, wrinkles are thicker, deeper and more numerous than natural aging. Clinically, it is characterized by dryness of the skin, deposition of pigment and expansion of peripheral blood vessels, histologically, cellular atypia, reduction of collagen, loss of elastic fibers (Lee, et al., 2014).

Changes in skin thickness caused by skin aging differ depending on natural aging and photoaging. In the case of natural aging, the thickness of the skin is decreased due to the decrease of the connective tissue such as collagen. However, in the case of photoaging due to ultraviolet light, the thickness of the skin becomes thicker in order to protect the dermal layer. The thickening of the skin due to photoaging due to ultraviolet light means that the skin damage due to photoaging is large (Gail J., 2002).

In recent years, the amount of ultraviolet rays reaching the ground surface due to environmental pollution has been increasing year by year, but outdoor activities for hobbies are increasing due to improved quality of life. Accordingly, there is a growing interest in various methods for preventing skin aging caused by external stimuli and for promoting skin health.

Golden ( Scutellaria baicalensis ) is a perennial plant of the dicotyledonous plant, monocotyledonous plant, and is widely distributed in Korea, China, and Siberia. It is also called spiny grass, almond, and fragrance. The golden roots are used as acupuncture, diuretic, branch, ejaculatory and anti-inflammatory agents in one room. It is known that baicalin and wogonoside, which are flavonoid-based glycosides, and baicalein and wogonin, which are non-glycosides, are present as major active ingredients of gold It is known that the non-glycoside form is more bioactive than the glycoside. In recent years, there have been conducted studies on the efficacy, such as the effective ingredients having liver protection, cirrhosis inhibiting, suppressing the above damage due to alcohol effects, gastric acid inhibiting, Helicobacter pylori (Helicobacter pylori) bacteria inhibited, joints and pain relief effect.

Leatiporus sulphureus var. Miniatus is a member of the mushroom family, and it is distributed throughout Korea, Mt. Paekdusa, Wolchulsan, Jirisan, and Baewangsan. It spreads throughout Japan and Asia. From spring to summer, it is a single-year-old heartwood of coniferous trees, dead trees, and regenerated stumps. Red duck mushrooms have long been reported to be effective in controlling function, promoting health, and preventing disease, but the exact pharmacological effect is not well known. Red foxtail mushroom fruiting bodies include N-methylated tilamine derivatives, polysaccharides, lanosthenate triterpenoids, retinoic acid and various other mixtures. Recently, it has been reported that red mushroom has anticancer effect and insulin secretion regulating effect. Anticancer effect shows that extracellular polysaccharide of red mushroom shows inhibition of cancer metastasis and enhancement of immunity. In addition, the extracellular polysaccharide of red mushroom mycelium Is known to promote the production of skin cell growth and has wound healing and skin moisturizing effects. In particular, the red mushroom is known to produce a large amount of functional enzymes including glucosidase.

The inventors of the present invention induced skin photoaging through ultraviolet irradiation in the process of studying the applicability of cosmetics to gold extracts, and found that the thickening of skin, which is one of the phenomena of skin photoaging, is caused by the fermentation of golden extracts into reddissolved mushroom mycelium Confirming that one fermented product is inhibited, thereby completing the present invention.

Conventionally, Korean Patent No. 1521239 discloses a cosmetic composition for preventing skin aging and improving skin wrinkles containing a fermented product of golden red mildew as an active ingredient. However, in the case of skin such as change in skin thickness due to photoaging of the present invention The effect of inhibiting damage is not described. Korean Patent No. 1375137 and No. 1545552 disclose a skin moisturizing effect and a skin whitening effect containing a fermented product of reddissume mushroom mycelium of gold extract, but the effect of inhibiting the skin thickness change by photoaging of the present invention .

Korean Patent No. 1521239, a cosmetic composition for preventing skin aging and improving skin wrinkles, which contains a fermented liquid of golden red mildew as an active ingredient. Registration May 05, 2015. Korean Patent No. 1375137, Cosmetic composition for moisturizing skin containing a fermented red mildew of golden extract. 2014. 03. 11. Registration. Korean Patent No. 1545552, a cosmetic composition for whitening skin comprising an active ingredient of a fermented liquid of golden red mildew. 08. 12. 12. Registration.

Nam, Jinju, et al., The effect of extracts and fractions thereof on skin photostabilization, J. Soc. Cosmet. Sci. Korea, 40 (4), 321-330, 2014. Lee, JH (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea); Gail J., Molecular mechanism of skin aging, Mech. Ageing Dev., 123, 801-810, 2002. Lee J. M., et al., Anti-wrinkle effect of Morinda citrifolia (Noni) extracts, J. Soc. Cosmet. Scientists Korea, 32, 227-231, 2006.

It is an object of the present invention to provide a cosmetic composition for inhibiting skin damage caused by ultraviolet rays containing a fermented product of reddissume mushroom mycelium of gold extract.

The present invention relates to a cosmetic composition for inhibiting skin damage caused by ultraviolet rays containing fermented mycelium of Leutipurus sulphureus var. Miniatus ( Scutellaria baicalensis ) extract.

The gold extract may be an extract of gold extracted with C1 to C4 lower alcohols as a solvent.

The fermented product of the reddish mushroom mycelium of the gold extract may be one obtained by inoculating mycelium of red mushroom with a fermentation medium prepared by mixing gold extract with purified water.

The fermentation medium may be a mixture of 100 parts by weight of purified water and 0.1 to 20 parts by weight of a gold extract.

The skin damage may be caused by thickening of the skin due to ultraviolet rays.

Also, the present invention relates to a cosmetic composition for inhibiting skin damage caused by ultraviolet rays, wherein the fermented product of reddissume mushroom mycelium of gold extract is contained in the total cosmetic composition in an amount of 0.001 to 30% by weight.

In addition, the present invention relates to a cosmetic composition containing a cosmetic composition for inhibiting skin damage caused by ultraviolet rays containing a fermented product of reddissume mushroom mycelium of the gold extract.

Hereinafter, the present invention will be described in detail.

The present invention relates to a cosmetic composition for inhibiting skin damage caused by ultraviolet rays containing fermented mycelium of Leutipurus sulphureus var. Miniatus ( Scutellaria baicalensis ) extract.

The gold extract may be an extract of gold extracted with a lower alcohol of C1 to C4 as a solvent, and the lower alcohol of C1 to C4 may be selected from ethanol, methanol, propanol, isopropanol and butanol. It is preferably ethanol, more preferably 70% [v / v] ethanol.

The gold extract is prepared by adding an extracting solvent of 1 to 20 times the weight of gold to gold and immersing it at room temperature for 3 to 7 days to prepare an extract. After removing the solid content from the extract, collecting the filtrate, the filtrate is concentrated under reduced pressure And dried by a method such as freeze-drying, spray drying, cold air drying, hot air drying, and the like.

The fermented product of the reddish mushroom mycelium of the gold extract can be prepared by inoculating a fermentation medium prepared by mixing the golden extract with purified water and inoculating the red mushroom mycelium to ferment the medium. 0.1 to 20 parts by weight of the extract may be mixed and sterilized.

At this time, the gold extract may be mixed with purified water in an aqueous solution state.

The fermented product of the reddish mushroom mycelium of the golden extract may be the fermented product containing the mycelium of reddissume mushroom or the precultured fermented product containing 0.001-10% by weight of the culture solution based on the fermentation medium.

The present invention also provides a method for producing a golden extract, comprising: a first step of preparing a gold extract; A second step of fermenting the golden extract with red mycelial fungi; And removing the reddish brown mushroom mycelium from the fermentation product. The present invention also provides a method for preparing a fermented product of a reddish mushroom mycelium of gold extract.

The gold extract of the first step may be used in the form of a concentrate or a dried form, and may be concentrated or dried, and then suspended in purified water to prepare an aqueous solution.

The fermentation of the gold extract in the second step may be performed using a fermentation medium prepared by mixing 0.1 to 20 parts by weight of the gold extract with 100 parts by weight of purified water.

The skin damage may be a phenomenon of photoaging that occurs when the skin is exposed to ultraviolet rays, and the thickness of the skin may become thick due to skin damage due to photoaging.

The thickening of the skin thickness may be due to an increase in the stratum corneum formation to protect the damaged skin when the skin is damaged by ultraviolet rays.

The fermented product of the reddish mushroom mycelium of the gold extract may be contained in the total cosmetic composition in an amount of 0.001 to 50% by weight, preferably 0.001 to 30% by weight.

The cosmetic composition of the present invention may contain, in addition to the fermented product of the red mildew of the golden extract of the present invention, other components capable of enhancing the skin damaging inhibitory effect by the ultraviolet rays of the present invention.

The fermented product of the red mildew of the golden extract may be used as a cosmetic material in a liquid state immediately after fermentation or as a dried material dried by a conventional drying method.

The drying method may be vacuum drying, freeze drying, spray drying, or cold air drying.

The cosmetic composition according to the present invention can be used as an adjuvant commonly used in cosmetics such as hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic active agents, preservatives, antioxidants, solvents, fragrances, fillers, ≪ / RTI >

The amount of the adjuvant is an amount ordinarily used in the art, for example, 0.001 to 70% by weight, preferably 0.001 to 50% by weight based on the total weight of the cosmetic composition. However, in any case, the adjuvants and their proportions may be chosen so as not to adversely affect the desired properties of the cosmetic composition according to the invention.

The cosmetic composition may be selected from lotion, gel, oil-soluble liquid, cream, ointment and essence.

The present invention relates to a cosmetic composition for inhibiting skin damage caused by ultraviolet rays containing fermented mycelium of Leatiporus sulphureus var. Miniatus ( Scutellaria baicalensis ) extract, wherein a fermented product of a reddish-purple mushroom mycelium of gold extract It was confirmed that the thickness of the skin due to skin damage during photoaging due to ultraviolet rays is suppressed.

Accordingly, it is expected that a cosmetic composition having an effect of suppressing skin damage due to excellent ultraviolet rays can be developed by using the fermented product of the red mildew of the golden extract of the present invention.

FIG. 1 shows the effect of suppressing the thickening of the skin thickness induced by the ultraviolet irradiation of the fermented product of the reddissume mushroom mycelium of the golden extract of the present invention. A shows the result of measuring skin thickness by H & E staining of dorsal skin tissue of a mouse irradiated with ultraviolet rays, and B shows the result of measuring the thickness of back skin of mouse.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein but may be embodied in other forms. Rather, the intention is to provide an exhaustive, complete, and complete disclosure of the principles of the invention to those skilled in the art.

≪ Example 1: Preparation of golden extract >

Gold was purchased from Jecheon herb medicine.

100 g of dried commercial gold was thoroughly washed, finely crushed, 1 l of 70% [v / v] ethanol was added, and the extract was extracted by immersion at room temperature for 5 days. The extract was filtered with a membrane filter having a pore size of 0.45 mu m, concentrated under reduced pressure, and then lyophilized to give 5.32 g of a yellow powdery golden extract.

≪ Example 2: Culture of mycelia of red foxtail mushroom &

The early mushroom mycelium (CS0218, KFCC 1149P) of the red mushroom ( Leatiporus sulphureus var. Miniatus) was distributed at Jeonnam Forest Resources Research Institute.

The mycelium of reddish brown mushroom was inoculated on a PDA (potato dextrose agar) slope medium, cultured at 25 ° C. for 6 days, stored at 4 ° C. until use, and subcultured at intervals of 2 weeks.

The seed culture for fermenting the golden extract was inoculated into 200 ml of PDB (potato dextrose broth) medium and cultivated for 7 days with stirring at 150 rpm at 25 ° C.

≪ Example 3: Preparation of fermented mycelium of reddish brown mushroom of golden extract >

The fermented mycelia of reddish brown mushroom of the golden extract of the present invention was prepared by culturing mycelia of red foxtail mushroom in a fermentation medium containing a golden extract.

To prepare a fermented product, 500 mg of the golden extract of Example 1 was added to 200 ml of purified water, and the resulting solution was sterilized under reduced pressure at 121 캜 and 2 atm for 15 minutes to prepare a fermentation medium.

Thereafter, 5 ml of the cultured Mycorrhizal fungus cultured in Example 2 was added to 200 ml of the fermentation medium, and the mixture was incubated at 25 ° C and 150 rpm for 7 days to ferment the golden extract. After the fermentation, the supernatant, from which all the mycelia of red duck mushrooms were removed by centrifugation, was lyophilized to prepare a fermented product of reddish mushroom mycelium of the golden extract of the dried state.

< Experimental Example  One. Experimental animal  Breeding>

Animal experiments were carried out in order to confirm the skin-damaging activity of the golden extract of the present invention on the ultraviolet light of the fermented mycelia of red mildew. Male HR-1 hairless mice at 6 weeks of age were used.

Mice were purchased from SLC, Inc. of Japan and used for the experiment after having a ballast for one week. The temperature of the mice was maintained at 24 ± 1 ° C and the humidity was maintained at 50%. The mice were allowed to eat food and water for 12 hours and 12 hours at night.

<Experimental Example 2> Confirmation of Skin Damage Suppression Activity by Ultraviolet Light>

Experimental Example 2-1. Sample administration and induced skin photoaging

In order to confirm the skin damage-inhibiting activity of ultraviolet rays of the fermented product of the red mildew of mycelia of golden extract of the present invention, the experiment group was divided into experimental groups corresponding to Table 1 below. The mice of Experimental Example 1 were randomly divided into three mice per experimental group.

The fermented product of the gold extract of Example 1 or the gold extract of Example 3 was dissolved in 0.5% ethanol containing 0.4% polyethylene glycol, and then the mixture was cultured for 12 weeks, 5 days a week , And 100 mg / kg on the back of the mouse. In addition, ultraviolet B was irradiated to the back of the mouse to induce skin damage. Ultraviolet B was irradiated three times for 12 weeks and irradiated at 60 mJ / cm 2 for 1 to 3 weeks, 90 mJ / cm 2 for 4 to 6 weeks and 120 mJ / cm 2 for 7 to 12 weeks for 5 to 10 minutes.

Experimental group Treatment condition Normal group Normal group Do not process anything Control 1 Induced skin injury UV B treatment Control group 2 Golden extract treatment group The gold extract of Example 1 and ultraviolet B treatment Experimental group Of gold extract
Fermented Mycelium of Red Duck Mushroom The gold extract of Example 3
Fermented products of mycelia of reddish brown mushroom
UV B treatment

Experimental Example 2-2. Confirm skin-damaging activity

When photoaging is caused by ultraviolet rays, the thickness of the skin becomes thick, and when the thickness of the skin becomes thick, it means that the damage of the skin is great. Therefore, in order to confirm the skin damaging activity of ultraviolet rays of the fermented product of the red mildew of mycelium of gold extract of the present invention, the change of skin thickness was observed.

The dorsal skin of the mouse of Experimental Example 2-1 was detached and fixed with 10% formalin to prepare a paraffin block. The prepared paraffin block was sectioned to a thickness of 5 mm to prepare a tissue section.

The tissue slices were stained with hematoxylin and eosin (H & E) staining, and the tissue thickness was observed using a microscope. The thickness of the skin was measured and the skin thickness was measured using an eyepiece micrometer. The results are shown in Fig.

The results of the skin texture observation (A) and the skin thickness measurement (B) of FIG. 1 showed that the thickness of the skin became thicker in the control group 1 irradiated with the ultraviolet ray B compared with the normal group, but the reddissume mushroom mycelium In the case of the experimental group irradiated with ultraviolet ray B, it was observed that thickening of the skin thickness due to ultraviolet ray B irradiation was suppressed. In addition, it was confirmed that the skin thickening inhibiting effect of the fermented product of the red mildew of mycorrhizal fungus extract of the present invention was higher than that of the gold extract (control 2).

Thus, it was found that the fermented product of the mycelium of red mildew of the golden extract of the present invention is excellent in the effect of suppressing skin damage caused by ultraviolet rays.

&Lt; Preparation Example 1: Preparation of cosmetic preparation >

Manufacturing example  1-1. Cream manufacturing

4 g of sitosterol, 3 g of polyglyceryl 2-olate, 0.7 g of ceramide, 2 g of cetearate-4, 3 g of cholesterol, 0.4 g of dicetyl phosphate, and concentrated glycerin were added to 5 g of the fermented product of the reddissume mushroom mycelium of Example 3, 22 g of Sunplaar oil, 0.5 g of carboxyvinyl polymer, 0.5 g of triethanolamine, a small amount of preservative and perfume, and purified water were mixed to a total weight of 100 g, and a nutritional cream was prepared according to a conventional cream preparation method.

Production Example 1-2. Lotion manufacturing

0.05 g of polypyrrolidone, 0.1 g of oleyl alcohol, 0.2 g of polyoxyethylene monooleate, 0.2 g of perfume, 0.1 g of p-hydroxybenzoic acid methyl ester, a small amount of antioxidant and a small amount of dye were mixed and dissolved in 8 g of 95% ethanol. 0.05 g of the fermented product of the reddissume mushroom mycelium of Example 3, and 5 g of glycerin were dissolved in 85.33 g of purified water. The mixture was added to the mixture and stirred to prepare a lotion containing the extract of the mixed herb.

Formulation Example 1-3. Emulsion manufacturing

1.2 g of cetyl alcohol, 10 g of squalane, 2 g of vaseline, 0.2 g of p-hydroxybenzoic acid ethyl ester, 1 g of glycerin monoestearate, 1 g of polyoxyethylene (20 mols of addition) monooleate and 0.1 g of perfume were heated and mixed at 70 캜 , 0.5 g of the fermented product of the reddish brown mushroom mycelium of Example 3, 5 g of dipropylene glycol, 2 g of polyethylene glycol-1,500, 0.2 g of triethanolamine and 76.2 g of purified water were dissolved by heating at 75 캜. The mixture was emulsified by mixing them and then cooled to prepare oil-in-water (O / W) type emulsion.

Formulation Examples 1-4. Serum preparation

To 5 g of 95% ethyl alcohol, 1.2 g of polyoxyethylene sorbitan monooleate, 0.3 g of quitrose, 0.2 g of sodium hyaluronate, 0.2 g of vitamin E-acetate, 0.2 g of sodium permanganate, 0.1 g of paraoxybenzoic acid ethyl ester, 1 g of the fermented product of the reddissume mushroom mycelium of the gold extract of Example 3 and an appropriate amount of pigment were mixed to prepare a serum.

Claims (7)

( Leatiporus sulphureus var. Miniatus) extract of Scutellaria baicalensis ( Mycobacterium tuberosum L.) from a fermented mycelium. The method according to claim 1,
Wherein the gold extract is a gold extract obtained by extracting gold from C1 to C4 lower alcohol as a solvent. The method according to claim 1,
The cosmetic composition for inhibiting skin damage caused by ultraviolet rays is characterized in that the fermented product of reddissume mushroom mycelium of the gold extract is fermented by inoculation with a mycelium of red mushroom in a fermentation medium prepared by mixing gold extract with purified water. The method of claim 3,
Wherein the fermentation broth is prepared by mixing 0.1 to 20 parts by weight of a golden extract with 100 parts by weight of purified water. 5. The method according to any one of claims 1 to 4,
Wherein the skin damage is thickened by ultraviolet rays. A cosmetic composition for inhibiting skin damage caused by ultraviolet rays, which comprises 0.001 to 30% by weight of a fermented product of reddissume mushroom mycelium of the gold extract according to claim 1 in the total cosmetic composition. A cosmetic comprising the composition of any one of claims 1 to 3.

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