KR100206538B1 - Skin whitening cosmetic compositions containing extracts of phellinus linteus powder - Google Patents

Abstract

The present invention relates to a situation mushroom extract and a skin cosmetic containing the same, which are excellent in terms of skin damage prevention and improvement effect, immune enhancing effect, whitening effect, antioxidant effect and excellent in terms of safety, water bath treatment water extract, As a mushroom extract extracted with a mixture of 60 to 95% ethanol extract, 1,3-butylene glycol and water, it is applicable as a component of skin cosmetics.

Description

Cosmetic composition for skin whitening containing situation mushroom powder extract

The present invention relates to a skin cosmetic containing the situation mushroom extract, which is particularly excellent in terms of skin damage prevention and improvement effect, immune enhancing effect, whitening effect, antioxidant effect, and also excellent in terms of safety and can be applied as a component of skin cosmetic The present invention relates to a mushroom extract and a skin cosmetic containing the same, which are extracted with a mixture of water-treated water extract, 60-95% ethanol extract, 1,3-butylene glycol and water.

General external skin preparations include cosmetics, medicines and quasi-drugs. Especially, external skin preparations for the purpose of preventing and improving skin damage have been conventionally used in combination with various raw materials starting from raw materials extracted from natural products. For example, ginseng extract, yeast extract, extracts such as pine mushroom extract, natural polymers such as collagen, skin rejuvenating agents such as amino acids and vitamins, or moisturizing agents such as glycerin and 1,3-butylene glycol are used to prevent skin damage. And it has been used in skin cosmetics for the purpose of improvement.

However, these conventional materials have been used to prevent and improve skin damage, and are used for the prevention and improvement of skin damage based on the low amount of sebum and moisture in the facial skin. There were not enough points to get the effect. Therefore, development of raw materials having various effects and no irritation to the skin has been required.

Phellinus linteus (Ikekawa, T., GANN, 59: 155, 1968) is a mushroom belonging to the pine scaly family and grows in the roots of mulberry trees. According to the classification of the real Japanese fungus picture book (issued by a nursery teacher, 1974), co-authored with the brass tuber, and hometown Chaung, it belongs to the family of the mushroom family, phellinus yucatensis. murril).

On the other hand, in Chinese herbal medicine dictionary (Shanghai Science and Technology Publishing House), the name of the drug of the phellinus igniarius is called the situation, but according to the Chinese medicinal fungus written by the papa, the situation is the stem of the mulberry tree. The only thing that happens is the situation, and it has the scientific name propolyporus yucatensis murril. Felinus Igaliarius has been a very broad interpretation over a long history. According to Volume 1 of the Japanese Color Fungus Book by Chunchon Cheong-il, Propolyporous Yucatensis Muril has a different name of phellinus linteus and all parts except the sack surface of mulberry are yellow. It is called (桑 黃) and is said to have other names such as Sangsin (桑 腎), Sang (桑 耳), said life (桑奇 生).

It is a well-known fact that the situation that grows in nature has anti-tumor, but it is difficult to grow in nature and grow in the tree of mulberry, which is called the actual situation, and it is difficult to obtain with the decrease of mulberry itself. I'm growing. The stems, leaves, and roots of mulberry trees have been used in medicine and cosmetics fields for a long time. Recently, only the research on the anticancer effect with the situation mushroom has been conducted at home and abroad.

It is an object of the present invention to provide a situation mushroom extract and a skin cosmetic containing the same, which are excellent in terms of preventing and improving various skin damages and having excellent safety. That is, the present invention provides a situation mushroom extract that is applicable to skin cosmetics and exhibits excellent immune enhancing effect, whitening effect, and antioxidant effect when contained in cosmetics.

The situation mushroom extract of the present invention for achieving the above object is an extract extracted with a water bath treatment water extract of 60% mushroom powder, 60-95% ethanol extract or a mixture of 1,3-butylene glycol and water. In addition, the situation mushroom extract is applicable as a component of the skin cosmetic, wherein the content of the situation mushroom extract is preferably 0.05 to 20% by weight.

1 is a diagram showing the ultraviolet absorption spectrum of the situation mushroom, arbutin, kojic acid, matsutake extract.

In the present invention, the effect that can be used in cosmetics from the water-treated water extract from the state of artificial cultivation in Korea, and the situation mushroom extract extracted with a mixture of 60-95% ethanol extract, 1,3-butylene glycol and water And when contained in the cosmetics have been found excellent antioxidant effect, immune enhancing effect, whitening effect, etc. to complete the present invention.

First of all, to confirm the availability of the skin cosmetics of the situation mushroom extract, the immune enhancing effect, the whitening effect and the antioxidant effect were investigated. The situation mushrooms were artificially cultivated in Korea, and the mushrooms were dried until there was no change in weight at room temperature, and then frozen in a nitrogen tank and ground into a mortar to make powder. The situation mushroom extract extracted with the mixture of water-treated water extract of 60% mushroom water, 60-95% ethanol extract, 1, 3- butylene glycol, and water was used as a sample.

As a result of UV absorption spectrum analysis, the situation mushroom extracts had strong UV absorption ability. In addition, the effect of free radical scavenging was examined as an effect test for application to skin cosmetics. As a result, it showed the highest free radical scavenging effect at 2% by weight sample concentration. The sample concentration (SC ₅₀ ) required to reduce 50% of the 1,1-diphenyl-2-picrylhydrazyl methanol concentration was 0.23% by weight and was superior to any other material in effect.

In the antioxidant effect test using linoleic acid autooxidation, the ethanol extract of situation mushroom had better antioxidant effect (46%) than vitamin E (42.3%), which has excellent antioxidant effect.

The level of superoxide anion produced by activated macrophage in the abdominal cavity was measured to evaluate the degree of immunity enhanced by the situation mushroom extract.

Compared to the control group, the peroxide anion was increased by 1.2 to 1.8 times in the control group.

The whitening effect on the mushroom extracts was investigated by experiments of tyrosinase activity inhibitory activity, melanin production inhibition of Streptomyces bikiniensis, and melanin production inhibition of B16-F1 melanoma cells. As a result, the concentration (IC ₅₀ ) that inhibits tyrosinase activity by 50% was 0.5% or less, and the melanin production region of the microorganism was inhibited by 16 mm at a concentration of 0.005%. In addition, the minimum concentration of inhibiting melanin production of B16-F1 melanoma cells was 0.134%, showing an excellent effect as a skin external preparation, especially skin cosmetics.

Application examples of the skin cosmetic containing the situation mushroom extract of the present invention has not been reported to date. The amount of this situation mushroom extract to skin cosmetics was not limited, but 0.05 to 20% by weight was found to be appropriate, and the effect was not expected at too small content.

In addition, the extract of the present invention is easily mixed with water and difficult to blend with fats and oils and organic solvents, but can be blended into the skin cosmetic even in a suspended state and has no effect on the effect. The external skin preparation of the present invention is suitable to be used as a skin cosmetic, and common ingredients such as oil, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, fragrances, etc. It is possible to combine them as necessary. In the present invention, the skin cosmetic is used for the skin, for example, a cosmetic, such as a lotion, milky lotion, cream, pack, and the application in particular to the whitening cosmetics.

Hereinafter, the present invention will be described in more detail with reference to Examples.

These examples are merely illustrative of the present invention and the scope of the present invention is not limited to these examples.

Example 1

Situation mushrooms were grown in Chuncheon, Gangwon-do, Korea. To use them as samples, dried mushrooms at room temperature until there is no change in weight, and then put them in a liquid nitrogen tank to freeze and crumble them. It was made into powder.

Three solvents were used for solvent extraction. The first is distilled water, the second is ethanol, and the third is a mixture of 1,3-butylene glycol and water.

Extraction by water was added to the situation mushroom powder at 5% concentration in water and water-bathed at 80 ℃ for 48 hours, and then filtered through a mesh mesh of 400 mesh size to separate the mushroom and the filtered liquid. Subsequently, the separated filtrate was sterilized by 0.45 μm filter paper and used as a sample.

Extraction by ethanol was added to 60%, 80% and 95% ethanol solution in water and 5% concentration of situational mushrooms were left at 25 ° C and 45 ° C for 15 days and filtered through a mesh mesh of 400 mesh. The filtrate was separated and the separated filtrate was filtered through 0.45 탆 filter paper and used as a sample.

In the extraction by the mixture of 1,3-butylene glycol and water, the ratio of 1,3-butylethylene glycol and water was mixed at a weight ratio of 8: 2 to 4: 6, and the situation mushroom was added at a concentration of 5% by weight of the mixture, and 40 After extracting by boiling water for 8 to 48 hours at 60, 80 ℃, filtered through a mesh mesh of 400 mesh size to isolate the mushroom and the filtrate, and then filtered the filtrate with 0.45㎛ filter paper to use as a sample.

Example 2

In this embodiment, the ultraviolet absorption of the material, which is a basic condition for the whitening effect, is investigated by the ultraviolet absorption spectrum.

1,3-butylene in the water-treated water extract of the situation mushroom extracted by the method of Example 1, the extract extracted with 95% ethanol for water in the ethanol extract, the extract extracted with a mixture of 1,3-butylene glycol and water Each of the extracts with a 6: 4 weight ratio of glycol and water was compared with arbutin, kojic acid, and pine mushroom extract, which are known to be superior to conventional UV protection.

The measurement method was compared by measuring the ultraviolet absorbance by using a UV spectrometer (Model: Cary, Company name: Varian (Australia)) by comparing the solvent dissolved in each sample. The various extracts (A) of the situation mushrooms absorb ultraviolet rays strongly in the wavelength range of 200-300 nm compared to 10% arbutin aqueous solution (B), 5% kojic acid aqueous solution (C), and pine mushroom ethanol extract (D), which are known as whitening agents. As shown in Fig. 1, a strong whitening effect was expected.

Example 3

In this example, the effect of free radical scavenging was observed.

0.2 ml of 1,1-diphenyl-2-picrylhydrazyl (1,1-diphenyl-2-picrylhydrazyl) methanol solution in various concentrations of water bath treatment, ethanol extract, 1,3-butylene glycol 1 ml of 10% ethanol solution of each extract extracted with a mixture of water and water was added, mixed, and then left at 37 ° C. for 10 minutes, and then the absorbance was measured at 517 nm. As a result, the free radical scavenging ability and the concentration (SC ₅₀ ) which reduces the free radical 50% were calculated as follows. As a result, the sample showed the maximum performance at about 2% by weight of the sample and SC ₅₀ was found to be 0.23% by weight (Table 1).

* Free radical inhibition rate (%) = [1- (E-B) / C] × 100

(B: control group, C: reference group, E: sample group)

* Scavenging ability (SC ₅₀ ): The size of the activity is expressed as the concentration (% by weight) of the sample required to reduce the concentration of 1,1-diphenyl-2-picrylhydrazyl methanol (66.7 μM) by 50%.

Example 3

In this embodiment, the degree of suppression of the automatic oxidation using linoleic acid, which is a good automatic oxidation material, is compared with each other. 3 ml of 10 mM linoleic acid solution and 0.075 ml of the sample adjusted to 4 mg / ml are added to an 8 ml cap tube, and the mixture is left at 4 ° C. After 0.1 ml of the mixed solution was added to 4.7 ml of 75% ethanol after 24 hours, the mixture was stirred, and then 0.1 ml of 30% ammonium thiocyanate solution and 0.1 ml of ferrous chloride reagent were added and left for 3 minutes, followed by color development. Absorption wavelength was measured and the antioxidant effect was measured in comparison with the control group. In the antioxidant effect test using linoleic acid automatic oxidation, the situation mushroom extract extracted in Example 1 was found to have an excellent antioxidant effect (46%) compared to vitamin E (42.3%) having excellent antioxidant effect (Table 2).

Example 5

This embodiment is to determine the whitening effect by looking at the degree of functional inhibition of the enzyme tyrosinase to determine the whitening effect of the situation mushroom extract extracted in Example 1.

Tyrosinase is an enzyme that accelerates the oxidation process of a substance called tyrosine in vivo to help produce melanin. In this example, a method of inhibiting the function of this enzyme and measuring the degree of inhibition of oxidization of tyrosine to form a black polymer called melanin (Pomerantz SH, J. Biochem., 24, 161-168 (1966)) Application was made to determine the whitening effect.

Morus extract (v / v% for distilled water of stock, 10, 1, 0.1, 0.01), situation mushroom extract (v / v% for distilled water of stock, 0.5, 0.05, 0.005, 0.0005), oil-soluble licorice extract, kojic acid Inhibition of tyrosinase activity was investigated using, arbutin and hydroquinone (v / v% of distilled water of 1, 0.1, 0.01, 0.001, 0.0001) as samples.

For inhibitory activity against tyrosinase, 15 μl of the sample was placed in a microplate (96 well), 150 μl of 0.1 M phosphate buffer (pH6.86) and 25 μl of 1.5 mM L-tyrosine solution were added, followed by 2,380 units / ml of mushroom tyrosinase ( 7 μl of Sigma, USA) (0.05 M phosphate buffer, pH 6.86) was added and reacted at 30 ° C. for 10 minutes, and measured at 490 nm using a microplate. Inhibition rate for tyrosinase (%) was calculated by the following equation, IC ₅₀ value is the concentration of inhibitors reaching 50% enzyme inhibition rate (Table 3).

% Inhibition = {[(D-C)-(B-A)] / (D-C)} × 100

A: absorbance before reaction of the inhibitor

B: absorbance after the reaction of the inhibitor

C: Absorbance before reaction of without inhibitor

D: absorbance after the reaction without the inhibitor

As a result of tyrosinase activity inhibitory test, the IC ₅₀ value of each extract of mushroom was less than 0.5%. Numerically, the inhibitory activity was found to be weaker than other substances, and it is considered to have an excellent effect considering that the extract is not a purely purified substance such as kojic acid or hydroquinone.

Example 6

This example was used to measure melanin production inhibitory action against actinomycetes (Streptomyces bikiniensis NRRL B-1049) was used by the Korea Institute of Science and Technology (KCTC) affiliated (KCTC 9172).

Actinomycetes were incubated at 28 ° C. for 2 weeks in papavizas' VDYA agar slant medium (V-8 juice 200ml, glucose 2g, yeast extract 2g, CaCO ₃ 1g, agar 20g, distilled water 800ml, pH 7.2) for 2 weeks. The spore suspension was then made with sterile water. ISP No. with 0.2% Yeast Extract 7 0.2 ml each of the spore suspension was applied to the plate medium, and then the amount of the sample was applied to a paper disk moistened with the sample on the surface of the medium with 30 µg / paper disk and incubated at 28 ° C. The melanogenesis inhibitory ring produced after 48 hours of cultivation was measured by using 4-hydroxyanisole known as a melanogenesis inhibitor as a control group to inhibit melanogenesis (Table 4).

Melanogenesis inhibitory activity against actinomycetes of each extract of S. aureus showed 16 mm melanin inhibitory effect in the case of S. mushroom ethanol extract. This inhibitory activity showed very good activity similar to that of 4-hydroxyanisole, a potent inhibitor of melanogenesis.

Example 7

This example was observed at the laboratory level to determine the skin whitening effect of the extract extracted with a water-treated water extract of ethanol, ethanol extract, a mixture of 1,3-butylene glycol and water.

B16 melanoma cells used in this example are cell strains derived from mice and cells that secrete a black pigment called melanin. Samples were treated during the artificial culture of these cells to evaluate the degree of melanin black pigment reduction. The B16-F1 melanoma cells used in this example were used by being sold from the American Type Culture Collection (Accession No .: 6323).

The inhibition of melanin biosynthesis of B16 melanoma cells was determined as follows. B16 melanoma cells were suspended in DMEM (Dulbecco's Modified Eagle's Medium (Gibco, USA) containing 10% fetal bovine serum at a concentration of 5 × 10 ³ cells / ml, 5 ml of suspension cells were placed in a tissue culture flask and assayed. Was added 0.134%, and cultured at 5 ° C.-95% air at 37 ° C. After 4 days of culture, the melanin production was first determined by the color of the culture medium, and the cells were washed with phosphate buffer, followed by trypsin treatment. The cells were collected in a tube, washed with phosphate buffer, and centrifuged to suppress the melanin secretion between several samples known as whitening agents and the situation mushroom extract. The evaluation method is that the color is changed according to the treatment of nothing in the cell culture and the degree of black melanin pigment secretion when each sample is treated. The degree was evaluated by visual observation are compared. As a result, Phellinus linteus extract to obtain a result of suppressing effect enables the melanin secretion.

Example 8

This Example was evaluated by quantifying the degree of secretion of peroxide anion produced by the macrophage activated in the mouse abdominal cavity to evaluate the degree of skin immunity by the situation mushroom extract extracted in Example 1.

Macrophage cells are one of the cells responsible for human immune system, and when stimulated by an external substance, they secrete peroxide anions to dissolve foreign substances or release them to the outside of the cell to protect the living body. In the immunoassay experiment, male, 9-week-old mice of the ICR system were used.

Four days later, the intraperitoneal macrophages were isolated by injecting thioglycolite, an anaerobic medium to activate the intraperitoneal cells, incubated for 48 hours in a 37 ° C, 5% carbon dioxide incubator, and in 96-well plates for immunoactivity experiment. The amount of nitrogen dioxide anion generated was measured at 4 × 10 ⁴ cells / well and 46 hours after sample treatment. As a control, interferon with high immunity effect and situation extract were added and mixed with 0.05 ~ 1.3% concentration of interferon, and the amount of nitrogen dioxide anion, a peroxide anion in the secretion of macrophage cells, was measured by absorbance spectrum at 540 nm with visible light absorption spectrum. .

As a result, when the interferon was added to the interferon by adding the situation extract to the interferon in the culture of macrophage cells, the secretion of nitrogen dioxide anion was about 2 times higher.

A: Mixture of water extract of situation mushroom and interferon

B) mixture of ethanol extract and interferon

C) A mixture of 1,3-butylene glycol and water extract of a situation mushroom and interferon

LA: interferon

[Examples 9, 10, 11]

In this example, comparative experiments of the cosmetics containing the respective situation mushroom extracts extracted in Example 1 were conducted.

The composition of the cream used in the comparative experiment is shown in Table 7. First, the phase b) recorded in Table 7 is heated and stored at 70 ° C. This was added a) phase, and after pre-emulsification, it was emulsified uniformly with a homomixer, and it cooled slowly, and the cream (Examples 9-11 and a comparative example) was prepared.

Each cream of Table 7 was applied to the right side of the face of five test subjects (20-35 year old female) twice a day for 1 consecutive month. After completion of the experiment, the skin color of the applied areas on both sides of the face was analyzed by image analysis, and the darkest color was set to 5, the middle color to 3, and the brightest color to 1. Table 8 compares the facial skin color of the experimenter using the cream of the composition of Example 10 with the facial use area of the experimenter using the cream of the composition of the comparative example. As shown in Table 8, it can be seen that the whitening effect appeared on the face of the experimenter applying the cream containing the situation extract.

Other examples are shown below. That is, as described above, it showed an excellent effect on skin improvement and a skin beauty effect.

Example 12

To 8 g of 95% ethanol, 0.05 g of polypyrroladon, 0.1 g of oleyl alcohol, 0.2 g of polyoxyethylene monooleate, 0.2 g of fragrance, 0.1 g of paraoxybenzoic acid methyl ester, a small amount of antioxidant, and a small amount of pigment are dissolved. 0.05 g of the situation mushroom extract of Example 1 and 5 g of glycerine were dissolved in 85.33 g of purified water, and the mixed solution was stirred and added to obtain a lotion having a skin improvement effect.

Example 13

1.2 g of cetyl alcohol, 10 g of squalane, 2 g of vaseline, 0.2 g of paraoxybenzoic acid ethyl ester, 1 g of glycerin monoesteralide, 1 g of polyoxyethylene (20 mole added) monooleate and 0.1 g of fragrance were mixed and dissolved at 70 ° C. 0.5 g of the situation mushroom extract of Example 1, 5 g of dipropylene glycol, 2 g of polyethylene glycol 1500, 0.2 g of triethanolamine, and 76.2 g of purified water were heated to 75 ° C. to dissolve it. Both are emulsified by mixing, followed by cooling to obtain an emulsion having a water / oil-based skin improvement effect.

Example 14

5 g of 95% ethyl alcohol, 1.2 g of polyoxyethylene sorbitan monooleate, 0.3 g of kitulose, 0.2 g of sodium hyaluronate, 0.2 g of vitamin E acetate, 0.2 g of sodium licorice, 0.1 g of paraoxybenzoic acid ethyl ester 1 g of the situation mushroom extract of Example 1 and a suitable amount of pigment is mixed to obtain a essence having an effect of improving the skin.

Situation mushroom extract according to the present invention is excellent in the prevention and improvement effect of various skin damage and very excellent in terms of safety. In addition, the above-mentioned situation mushroom extract is applicable to skin cosmetics and exhibits excellent immune enhancing effect, whitening effect and antioxidant effect when contained in cosmetics.

Claims (6)

Cosmetic composition for skin whitening, characterized in that it contains the extract of fruiting body fruit powder. The cosmetic composition for skin whitening according to claim 1, comprising 0.05 to 20% by weight of the fruit mushroom body extract. The cosmetic composition for skin whitening according to claim 1 or 2, wherein the situation mushroom fruiting body powder extract is extracted with a water bath treatment. The cosmetic composition for skin whitening according to claim 1 or 2, wherein the situation mushroom fruit body powder extract is extracted with 60 to 95% ethanol in water. The cosmetic composition for skin whitening according to claim 1 or 2, wherein the fruit mushroom fruit extract is extracted with a mixture of 1,3-butylene glycol and water. The method of claim 5, wherein the mixing ratio of 1,3-butylene glycol and water is 8; It is 2-4: 6 and heat-extracted at 40-80 degreeC, The cosmetic composition for skin whitening characterized by the above-mentioned.