KR100508528B1 - method for manufacturng the Skin Cosmetics using horse bean and Golden larch extract - Google Patents

Abstract

The present invention relates to a skin cosmetic using a mixed extract of soybean and Geumsong jeonchoe, among the skin improvement effect, in particular, to prepare a skin cosmetic excellent in skin whitening effect and anti-wrinkle effect, antioxidant effect, hair growth effect, anti-inflammatory effect, acne prevention effect will be.

To this end, in the present invention, the first step of preparing the soybeans and jinsongjeon to dry at room temperature until there is no change in weight; A second step of mixing and grinding the dried soybeans and the golden soybean paste at a ratio of 1: 1 by weight to obtain a mixed powder; The mixed soybean and calendula soybean powder obtained in the second step is 1: 2 by weight in any one aqueous solution selected from distilled water / 30-95% ethanol / methanol / 1,3-butylene glycol / 30% propylene glycol / glycerine. A third step of heating at 48 ° C. under a water bath at 60 ° C. for 48 hours; There is provided a method for producing skin cosmetic using a soybean and Geumsongchocho extract comprising a fourth step of filtering the heated material obtained in the third step with 0.45 μm filter paper.

Description

Method for manufacturng the Skin Cosmetics using horse bean and Golden larch extract}

The present invention relates to a method for preparing skin cosmetics using extracts of soybean and Geumsong jeoncho, in particular, the soybeans as an ingredient of skin cosmetics having excellent skin improvement effects such as skin whitening, anti-wrinkle effect, antioxidant effect, anti-inflammatory effect, hair growth effect, and acne prevention effect The present invention relates to a method for preparing skin cosmetics containing extracts of water, methanol, ethanol, 1,3-butylene glycol, propylene glycol, glycerin, and extracts thereof.

In general, external skin preparations include cosmetics, medicines, and quasi-drugs. In particular, external skin preparations for the purpose of preventing and improving skin damage are conventionally used in combination with various raw materials, starting with raw materials extracted from natural products. , Ginseng extract and licorice extract, extracts such as chajeoncho, natural polymers such as collagen, skin cell activators such as amino acids and vitamins, or moisturizing agents such as glycerin and 1,3-butylene glycol for the purpose of preventing and improving skin damage It has been used in skin cosmetics.However, in the use of materials that have been used to prevent and improve the skin damage, it is used to collect plants grown in nature and artificially cultivate the same materials. It is well known that the efficacy effect differs depending on the type (Proc, Jpn, Assoc, Mycotoxical, No. 30, 1989). In particular, in the case of plants, in order to obtain specific effects such as skin whitening effect, immune resiliency effect, and antioxidant effect, there was a problem that the effects vary greatly depending on the harvesting site, harvesting time, and location of the plant.

The soybeans used in the present invention is a scientific name Canavalia gladiata DC, a royal crop that has been used as a medicine or food only in the royal family of the Goryeo Dynasty, and is a native plant that has been banned due to the Koryo eradication policy since the Joseon Dynasty. Small beans are called pods or curds because the fruit is a bow-shaped pod that is shaped like a small bean.

The shape and characteristics of the pea beans are annual grasses, and the leaves are three long, egg-shaped leafy leaves, and have a triple overlapping shape. The flowers are light red or branched, and the navel length of the seeds is half the length of the seeds. The germplasm has three kinds of cultivars: white lip of white flower, red lip of white flower, and red lip of red flower, and it is a crop that is resistant to drought, strong acidity, sandy soil, and shady areas. It is widely distributed in Africa, India and cultivated within 1000m above sea level, and is also cultivated in Southeast Asia and Korea. The main components of pea beans are urease, hemagglutinin, canavarin, protein and oil, and hemagglutinin is composed of various globulins such as Concanavalin A. These ingredients are known to have a special effect on tumor cells. . In addition, there are glucosidase and arginase, and there are 3 to 5 times more vitamin B group, vitamin C, and niacin than other legumes.

The general known efficacy of small bean beans is known to be high in soybeans, along with rats and green beans. The white lip of white flowers is known to contain many healthy ingredients among three cultivars of small beans. The benefits are very diverse and outstanding, but here are some of the pharmacological benefits of soybeans: It has excellent efficacy in the treatment of purulent diseases, inflammation or ulcers caused by poor stomach and intestine, weakness of the waist pain due to weak kidneys, weakness of blood circulation, constipation, and obesity. There are very few domestic studies on the properties and specific functional ingredients of Korea, and the report on general ingredients (Korea Food Research Institute, 1995) is all. Herbal medicine uses hemagglutinin, diabetes, anti-cancer, hemorrhoids, etc. for empirical prescription (components and use of herbal medicine, 1991). Production associations are being formed (Dongyang Ilbo, December 11, 1997), and some farmers are promoting sales at department stores and exporting to Japan (Busan Ilbo, July 20, 1998). As a method of use, the traditional method of boiling in water and using the powder or powdering and taking it (Jo Kyu-hyung, 1998) is the only way to develop the use of simple and hygienic health foods, pharmaceuticals, beauty, etc., suitable for modern consumption. Therefore, in order to process soybeans, the first thing that needs to be done is to study the physical properties, textures, and structures of specific ingredients. Where it can be said that the need to make research has minimal loss of functional ingredients can be processed.

In the case of China, there are many studies on pharmacological action in oriental medicine, and it has been used for many years as a folk medicine used for therapeutic, post-mortem recovery, and nourishing tonic (Jo Kyu-Hyung, 1998). It has been used as a treatment for hemorrhoids and sinusitis (Japan Soybean Fund Association, 1995), and it is still commercially available in pharmacies as their treatment. Currently, structural analysis, pharmacological action and verification of bioactive substances are conducted in Japan and China. It is known that food development is also in progress. Seeds are distributed by Japanese seedling companies and are consumed for vegetables and seeds.

There are many reports on the general composition of peas, their specific structure, physicochemical properties, genetic properties, and the separation of specific components. The focus is on the impact of Therefore, in the present invention, the cosmetic side when the external appearance on the skin of the pea beans has been newly developed to reach the present invention.

Geumsong ( 학松) is scientific name Sciadopitys verticillata and is a plant of the Taxodiaceae. The etymology is that Sciadopitys is a Greek compound of skias and pitos, and verticillata is a returning plant. As the world's three largest landscape trees, even without pruning, the tree is a plant that is mainly used as landscape trees because it has valuables like sculptures.

There is no research on the efficacy of this plant on the human body.

In the present invention, the present invention has been studied as a result of studying the cosmetic aspects of the human body with this plant outpost.

Thus, it is true that soybeans and calendula have been mentioned various pharmacological effects, but since no extract thereof was used as a skin cosmetic in the cosmetic field, a method of manufacturing a method was required.

The present invention is to meet the above-mentioned conventional needs, an object of the present invention is to provide a manufacturing method for producing a skin cosmetic excellent in the prevention of various skin damage and skin improvement effect and excellent in terms of safety in use. .

In the present invention, the first step of preparing the soybeans and jinsongjeon soaked at room temperature until there is no change in weight; A second step of mixing and grinding the dried soybeans and the golden soybean paste at a ratio of 1: 1 by weight to obtain a mixed powder; The mixed soybean and calendula soybean powder obtained in the second step is 1: 2 by weight in any one aqueous solution selected from distilled water / 30-95% ethanol / methanol / 1,3-butylene glycol / 30% propylene glycol / glycerine. A third step of heating at 48 ° C. under a water bath at 60 ° C. for 48 hours; And a fourth step of filtering the heated material obtained in the third step with a 0.45 μm filter paper. 3-butylene glycol, propylene glycol, distilled water may be a mixed solution mixed in any ratio selected from the ratio of 4: 4: 2 to ratio of 2: 2: 6 by weight, i.e., from the bean and calendula mixed extract Water, Methanol, Ethanol, 1,3-Butylene Glycol, Propylene Glycol, Glycerin, and their extracts with mixed solvents have been proven to be effective in cosmetics and contain excellent antioxidant, whitening and antiwrinkle properties in cosmetics. The effect, anti-inflammatory effect, hair growth effect, etc. have been found to make the present invention. First, in order to confirm the applicability of the skin cosmetic of the soybean and calendula mixed extract, the whitening effect, antioxidant The anti-wrinkle effect, anti-inflammatory effect, hair growth effect and anti-acne effect were investigated. 100 g of the mixed powder obtained by adding 30% propylene glycol aqueous solution to 200 g of water at 60 ° C. for 48 hours, and then filtered with 0.45 μm filter paper. After 10 days in a 30% aqueous solution of ethanol at room temperature for 10 days, it can be sterile filtered to 0.45㎛ (㎛). In addition, 100g of mixed soybean and Geumsong jeonchocho powder is added to 200g of distilled water and treated by boiling water at 80 ℃ for 48 hours. After separating the green soybean and Geumsongcho powder filtrate with a mesh mesh of 400 mesh size, and then filtered with 0.45㎛ sterile filtration paper may be used as a sample. As an effect test for applying the extracts of soybean and Geumsongchoe as skin cosmetics, the ethanol extract of Soybean and Geumsongchoe in general is known to have an excellent anti-oxidizing effect in the antioxidant effect test using linoleic acid automatic antioxidant method. Phosphorus mushroom extract (45%) and vitamin Lee (42.3%) had superior antioxidant effects (72%).

Inhibition of tyrosinase activity, inhibition of melanogenesis of microorganisms ( Streptomyces bikiniensis ), and inhibition of melanogenesis of non-16- F1 melanoma cells were investigated. The concentration of 50% inhibiting the tyrosinase activity (IC ₅₀ ) was less than 0.03%, at a concentration of 0.002% inhibited the melanin production region of the microorganism 28 mm. In addition, the minimum concentration of inhibiting melanin production of B16-F1 melanoma cells was 0.02%, showing an excellent effect as an external preparation for skin, especially cosmetics. The amount of this soybean and calendula extract in skin cosmetics is not limited, but it is found to be 0.001 to 100% by weight, and it is difficult to expect the effect at too small content, and in some cases, the extract itself. The extract of the present invention is easily blended with water and difficult to blend with fats and oils and organic solvents. However, the extract of the present invention can be blended into the skin cosmetic even in suspension and has no effect on the effect. Skin external preparation of the invention is suitable to be used as skin cosmetics and general blood It is possible to apply and blend as usual ingredients, such as oil, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, fragrances, etc., as necessary. It can be applied, for example, to cosmetics such as lotion, milky lotion, creams, packs, and especially whitening cosmetics.

Hereinafter, the present invention will be described in more detail with reference to Examples.

These examples are merely illustrative of the present invention and the scope of the present invention is not limited to these examples.

Example 1

Ginseng and Calendula were grown on the Korean Peninsula, and for use as samples, dried Ginseng and Calendula at room temperature until there is no change in weight, and then pulverized by a general mechanical method to mix the two bean and calendula in the same amount. Used.

Various solvents were selected for solvent extraction, based on water, methanol, ethanol, 1,3-butylene glycol, propylene glycol and glycerin, respectively, and combinations thereof.

Extraction by water was put 50% by weight of the mixed soybean and Geumchochocho mixed powder in water, and the mixture was filtered in a mesh mesh of 400 mesh for 48 hours in water and separated from the soybean, the golden pine and the filtered liquid. Subsequently, the separated filtrate was sterilized by 0.45 μm filter paper and used as a sample. Extraction by ethanol was carried out in a 30% solution of ethanol in water and 50% of soybeans and calendulas at room temperature for 15 days, and then filtered through a mesh of 400 mesh to separate the soybeans, calendula and filtrate, and the filtrate was separated into 0.45㎛ Filtered with filter paper to prepare a sample and the efficacy effect test as in the following examples. In the extraction by the mixture of 1,3-butylene glycol, propylene glycol and water, the ratio of 1,3-butylene glycol, propylene glycol and water is mixed 8 to 2 and 4 to 6 by weight fertilizer, and the mixture is 50% by weight. After adding the green bean and the golden pine and heat treatment at 60 ℃ for 48 hours, and filtered through a mesh mesh that is 400 mesh, the green bean and golden pine mixed powder and the filtrate were separated, and the filtrate was filtered with 0.45㎛ filter paper to prepare a sample.

Example 2

This embodiment compares the degree to which the automatic oxidation is suppressed by using linoleic acid, which is a good automatic oxidation material. 3 ml of 10 mM linoleic acid solution and 0.075 ml of the sample adjusted to 4 mg / ml were added to an 8 ml cap tube, and the mixture was left at 4 ° C. After 0.1 ml of the mixed solution was added to the test tube containing 4.7 ml of 75% ethanol aqueous solution after 24 hours, the mixture was stirred, and 0.1 ml of 30% ammonium thiocyanate solution and 0.1 ml of ferrous chloride reagent were added and left to stand for 3 minutes. After color development, the absorption wavelength was measured at 500 nm, and the antioxidant effect of the substance was measured in% compared with the control group without the sample. In the antioxidative effect test using linoleic acid automatic oxidation, the mixed bean extract of Soybean and Geumsongchocho extract according to Example 1 had an excellent antioxidant effect compared to the control group and had an excellent antioxidant effect (72%) compared to vitamin E (42.3%). Appeared (Table 1).

Table 1

Example 3

Dense cell metabolism in the skin produces enough energy to activate cell differentiation. This serves to protect the skin from aging. As we get older and under stress, the cells decrease and their metabolism decreases. The skin loses its vitality and transparency. A user test with a cream containing a mixture of green beans and Geumsong jeonchoen showed a noticeable improvement. A micro wrinkle measuring device was used for 10 women aged 40 to 50 years to change the skin surface condition when using a water-in-oil (O / W) type cream containing 5% of soybean and Geumsong jeoncho powder twice a day. Skin visiometer 400, Germany) showed more than twice the improvement of fine wrinkles compared to cream without extract (Table 2).

The measurement area was measured around the eye area. After dividing in half with the center of the facial glaucoma, one side A and the other side B were applied to observe the effect of reducing skin wrinkles before and after 50 days of use.

TABLE 2

                                             (N = 10 average)

Example 4

This experiment was to determine the whitening effect by looking at the degree of functional inhibition of the enzyme called tyrosinase in order to determine the whitening effect of the mixed soybean and Geumsongchocho extract powder extracted in Example 1. Tyrosinase is an enzyme that helps melanin production by promoting the oxidation of a substance called tyrosine in vivo. In this embodiment, the method of inhibiting the function of this enzyme (Sigma (USA)) to inhibit the tyrosine oxidized to form a black polymer called melanin (Pomerantz SH, The tyrosine hydroxylase activity of mammalian tyrosinase, J. Biochem., 24, 161-168 (1966)) was applied as a whitening effect. Morus extract (volume / volume% for distilled water of stock solution, 10, 1, 0.1, 0.01), mixed powder extract (cereal, volume / volume% for distilled water of 0.5, 0.05, 0.005, 0.0005), high Inhibition of tyrosinase activity was investigated using direct acid, arbutin, hydroquinone and pi-methoxyphenol (volume / volume% of distilled water of 1, 0.1, 0.01, 0.001, 0.0001) as samples.

For inhibitory activity against tyrosinase, 152 μl of the sample was placed in a microplate (96 well), 150 μl of 0.1 mole phosphate buffer (pH6.86) and 25 μl of 1.5 mM L-tyrosine solution were added, followed by 2,380 unit / ml mushroom tyrosinase (0). 7 μl of .05 mol phosphate buffer, pH6.86) was added thereto, followed by reaction at 30 ° C. for 10 minutes. Inhibition rate for tyrosinase (%) was calculated by the following formula, IC ₅₀ value was determined by the concentration of inhibitors reaching 50% enzyme activity inhibition rate (Table 3).

% Inhibition = {[(D-C)-(B-A)] / (D-C)} × 100

A: absorbance before reaction of the inhibitor

B: Absorbance after the reaction with the inhibitor

C: Absorbance before reaction of without inhibitor

D: absorbance after the reaction without the inhibitor

As a result of inhibiting tyrosinase activity, the IC ₅₀ value of each extract of soybean and Geumsongchocho was less than 0.2%. It was more effective than situation mushrooms and had better effects than arbutin, which was previously known to have excellent whitening effects.

Table 3

Example 5

In this experiment, the strain ( Streptomyces bikiniensis NRRL B-1049) used to measure melanin production inhibitory activity against actinomycetes was distributed from KCTC (Korean Collection for Type Culture, Accession No. 9172).

( Streptomyces bikiniensis NRRL B-1049) was incubated at 28 ° C for 2 weeks in papavizas' VDYA agar slant medium (V-8 juice 200ml, glucose 2g, yeast extract 2g, CaCO₃1g, agar 20g, distilled water 800ml, pH7.2) for 2 weeks. Spores were produced to produce spores with sterile water. ISP No. added 0.2% yeast extract 7 0.2 ml each of the spore suspension was applied to the plate medium, and the amount of the sample was applied to the paper disk soaked on the surface of the medium with 30 ㎍ / paper disc and incubated at 28 ° C. The inhibition of melanogenesis was observed using the 4-hydroxyanisole known as the melanogenesis inhibitor as a control for the size of the melanogenesis inhibitors produced after 48 hours of incubation (Table 4).

The inhibitory activity of melanin production on Streptomyces bikiniensis NRRL B-1049 of the extracts of the soybean and Geumsongchoen extracts showed a melanin inhibition rate of 28 mm in the ethanol extract of the Soybean and Geumsongchoen mixed powders. This inhibitory activity was superior to 4-hydroxyanisole, a potent inhibitor of melanin production.

Table 4

Example 6

This experiment was observed at the cellular level to examine the skin whitening effect of extracts from water, methanol, ethanol, 1,3-butylene glycol, propylene glycol, and glycerin and their mixtures. .

The B16-F1 melanoma cells used in this experiment are cell strains derived from mice and secrete a black pigment called melanin. The cells were treated with a substance during artificial culture to evaluate the degree of reduction of melanin black pigment. The B16-F1 melanoma cells used in this experiment were distributed from ATCC (American Type Culture Collection, Accession No .: 6323). It was. Melanin biosynthesis inhibition of B16 melanoma cells was determined as follows. B16 melanoma cells were suspended in Dulbecco's Modified Eagle's Medium (Gibco, USA) containing 10% fetal bovine serum at a concentration of 5 × 10 3 cells / ml. Suspension cells (5 ml) were placed in a tissue culture flask, and 0.134% of the assay sample was added, followed by incubation at 37 ° C. under 5% carbon dioxide-95% air condition. Differentiation was performed, and the cells were washed with phosphate buffer and trypsinized to separate the cells from the flask. The cells were collected in a tube, washed with phosphate buffer, and centrifuged to compare the amount of melanin produced by the whitening agent and the melanin secretion inhibitory effect of several samples known as whitening agents and bean and calendula extracts. The evaluation method was evaluated by visual observation of the degree of color change according to the treatment of nothing in the cell culture and the degree of black melanin pigment secretion when each sample was treated. As a result, the soybean and calendula extracts effectively inhibited melanin pigment secretion (Table 5).

Table 5

Example 7, 8, 9

In this example, a comparative experiment was carried out for the cosmetics containing each of the soybeans and Geumsong jeoncho powder extract extracted in Example 1. The composition of the cream used in the comparative experiment is shown in Table 6. First, phase b) recorded in Table 6 is heated and stored at 70 ° C. (A) To this phase, pre-emulsification is carried out, and then emulsified uniformly with a homomixer, and then gradually cooled to a cream (Example 7-9, comparison). Example) Each cream of Table 6 was applied twice a day for 1 month on the right side of five test subjects (women aged 20-35 years). After completion of the experiment, the skins on both sides of the face were compared by using an image analyzer. The dark color was set to 5 neutral color 3 and the brightest color to 1, and the intermediate degree was estimated. Table 7 compares the facial skin color of the experimenter using the cream made in Example 7 with the facial use area of the experimenter using the cream made in the comparative example. As shown in Table 7, it can be seen that the whitening effect appeared on the face of the experimenter applying the cream containing the extracts of the soybean and Geumsong jeoncho mixed powder.

Table 6

Table 7

Example 10

After the mouse was applied to the back area with the epilation cream, hair removal was performed using gauze, and the hair growth rate was measured by applying a transparent gel containing 30% of the soybean and Geumsongchocho mixed powder extracts twice daily. The length of the hair over time was measured in 5 control groups and 5 test groups. The test group showed a hair growth rate of 75-90% at 15 days after application. On the other hand, the natural control group without the test substance showed 65% hair growth rate.

Table 8

Example 11

The living body always adapts to and responds to various environmental changes. When attacked by causing any organic change in tissues to a reversible response that maintains homeostasis, a nonreversible response is produced for the purpose of defending and restoring the body. This dynamic sequence is inflammation. Fever, pain and edema resulting from the inflammatory process are pain. In addition, bioreactions may be overprotective, inflammatory reactions may threaten life, and inflammatory reactions may proceed even though the cause of inflammation has already disappeared. It is useful to suppress it. In diseases with reduced inflammatory response, inflammation is required. Therefore, anti-inflammatory drugs are needed to suppress such inflammation. Based on these facts, it is required to develop cosmetics having excellent efficacy by adding an anti-inflammatory material.

Methods for evaluating anti-inflammatory effects include measuring the inhibitory effect of hyaluronidase and animal testing using guinea pigs.

In the present invention, the inhibition of hyaluronidase enzyme activity was measured to investigate the anti-inflammatory effects of the extracts of the soybean and Geumsong jeoncho mixed powder. Hyaluronidase is one of several factors involved in the inflammatory response mechanism, which is activated by histamine, which is secreted from mast cells, which causes inflammation. The activity of the enzyme by the enzyme-substrate reaction between the lon acids is measured by absorbance. Experiments were made in the sample shown in Table 9 when the following procedure was performed to obtain the results as shown in Table 10 to prove the excellent anti-inflammatory effect of the bean and calendula extract.

Table 9

end. Add A and B and react for 20 minutes at 37 ° C, b. Add C and react at 37 ℃ for 20 minutes

All. Add D and react at 40C for 40 minutes. Add E and F and react for 3 minutes at 95 ℃

hemp. Cooldown, bar. Add G and react at 37 ° C for 20 minutes (p-DABA diluted 10-fold with Acetic acid when used). Absorbance measurement at 585 nm (Blank is held in Acetate buffer)

Table 10

Example 12

Acne formation is associated with abnormalities in the sebaceous glands, a pathological condition. That is, it is determined by various factors, and the sebaceous gland activity is affected by the rate of sebum production and the composition of sebum by hormonal regulation. The sebaceous glands are particularly sensitive to the hormone androgen, which is involved in the increase of sebum secretion, because excessive skin sebum in acne tends to be increased by dihydrotestosterone, a testosterone hormone called 5 α- reductase. Acne is also one of the causes of propionibacterium acnes , a skin fungus, which breaks down triglycerides of sebum into fatty acids and glycerol by lipases, which cause inflammatory reactions.

In the present invention was examined the extent to inhibit the production of acne by measuring the effect of inhibiting the growth activity of the skin fungus Propionibacterium acnes . In the test method, Propionibacterium acnes (KCTC 3314) was incubated in saline medium (5.5 g / L NaCl, 2.8 g / L KH₂PO₄g / L) containing pancreatic peptone (5.6 g / L). Those containing and not containing the starch mixed powder extract and cultured with 5 g / L of glucose as a carbon source medium were incubated, respectively. The amount of differentiated bacteria was evaluated by measuring turbidity at 750 nm after 48 hours of incubation. As a result, 40% inhibition in culture containing 2% of soybean and Geumsong jeoncho extract and 70% in 8% culture effectively inhibited the growth of bacteria.

Other examples are shown below. That is, as shown above, it showed an excellent effect on skin improvement and a skin beauty effect.

Example 13

To 8 g of 95% ethanol, 0.05 g of polypyrrolidone, 0.1 g of oleyl alcohol, 0.2 g of polyoxyethylene monooleate, 0.2 g of fragrance, 0.1 g of paraoxybenzoic acid methyl ester, a small amount of antioxidant, and a small amount of pigment are dissolved. . This was added with stirring in a mixture of soybean and Geumsong jeoncho powder powder 0.05g and glycerin 5g in purified water 85.33g to obtain a lotion having a skin improvement effect.

Example 14

1.2 g of cetyl alcohol, 10 g of squalane, 2 g of vaseline, 0.2 g of paraoxybenzoic acid ethyl ester, 1 g of glycerin monoesteralide, 1 g of polyoxyethylene (20 mol added) monooleate and 0.1 g of fragrance at 70 ° C. And 0.5 g of the mixed powder extract of Geumcho jeoncho, 5 g of dipropylene glycol, 2 g of polyethylene glycol 1500, 0.2 g of triethanolamine, and 76.2 g of purified water were heated to 75 DEG C and dissolved. Both are emulsified by mixing, followed by cooling to obtain an emulsion having a water / oil-based skin improvement effect.

Example 15

5 g of 95% ethyl alcohol, 1.2 g of polyoxyethylene sorbitan monooleate, 0.3 g of seaweed extract, 0.2 g of sodium hyaluronate, 0.2 g of vitamin acetate, 0.2 g of sodium licorice, 0.1 g of paraoxybenzoic acid ester, 1 g of calendula mixed powder extract and a suitable amount of pigment are mixed to obtain a cosmetic liquid having an effect of skin improvement.

The soybean and Geumsong jeoncho extract obtained by the present invention are excellent in various skin improvement effects, in particular, the skin whitening effect, anti-wrinkle effect, antioxidant effect, anti-inflammatory effect, hair growth effect, acne prevention effect provides a very good useful effect. The present invention, the above-mentioned soybean and Geumsongchocho mixed powder extract is applicable to skin cosmetics and also provides other effects to obtain an excellent skin improvement effect when contained in cosmetics.

Claims (7)

A first step of preparing each of soybeans and golden soybean paste at room temperature until there is no change in weight; A second step of mixing and grinding the dried soybeans and the golden soybean paste at a ratio of 1: 1 by weight to obtain a mixed powder; The mixed soybean and calendula soybean powder obtained in the second step is 1: 2 by weight in any one aqueous solution selected from distilled water / 30-95% ethanol / methanol / 1,3-butylene glycol / 30% propylene glycol / glycerine. A third step of heating at 48 ° C. under a water bath at 60 ° C. for 48 hours; And a fourth step of filtering the heated material obtained in the third step with a 0.45 μm filter paper. delete delete delete The aqueous solution of claim 1, wherein 1,3-butylene glycol, propylene glycol, distilled water, soybean and Geumjeonchocho extract, characterized in that the mixture is mixed in any ratio selected from the ratio of 4: 4: 2/2 :: 6 ratio by weight Skin cosmetic preparation method using. delete delete