KR100909574B1 - Cosmetic Composition Comprising Polygonum bistorta extract for Free Radical Scavenging Activity - Google Patents

Abstract

The present invention relates to a cosmetic composition for free radical scavenging containing a ginseng extract. The cosmetic of the present invention may be prepared in the form of softening (skin), astringent makeup, nutrient cosmetics (lotion), nutrition cream, massage cream, essence or pack. Free radical scavenging cosmetics containing the ginseng extract of the present invention by eliminating the free radicals generated in the skin, by inhibiting the oxidation of the biological material by the free radicals to delay the prevention of aging of the skin and is safe because it is a natural substance.

Description

 Cosmetic composition for free radical scavenging containing ginseng extract {Cosmetic Composition Comprising Polygonum bistorta extract for Free Radical Scavenging Activity}

The present invention relates to a cosmetic composition for free radical scavenging containing one or more extracts selected from the group consisting of Hyangbuja, Geumchocho, Hagocho, Yakja, Taekran, Kwonsam, Red Peony, and Asil. The present invention relates to cosmetics for free radical scavenging that are excellent in preventing or retarding skin aging and being a natural substance.

In general, cosmetics keep the skin beautiful and healthy, and delay the aging of the skin as it ages. Aging of the skin is due to oxidative damage of biological material by free radicals. In other words, highly reactive free radicals such as hydrogen peroxide, hydroxy radicals, superoxide radicals, and other active oxygen radically oxidize the lipids that make up cell membranes, destroy cell membranes, oxidize proteins, and attack nucleic acids, causing oxidative damage. Destroys and eventually kills the cell. Thus, preventing or delaying aging of the skin is made possible by eliminating free radicals in the skin. Ascorbic acid, DL-alpha-tocopherol, beta-carotene, and dibutyl hydroxyltoluene (BHT) are known to have free radical scavenging effects. have. Because these substances are mainly used as synthetics, they can cause skin safety problems for long-term use or in large amounts. Therefore, there is an increasing demand for free radical scavenging cosmetics that are safer for skin application.

The present inventors studied various plant extracts in order to search for substances that prevent skin aging, and as a result, extracts obtained from branches or leaves such as Hyangbuja, Geumchocho, Hagocho, Yakja, Taklan, Ginseng, Red Peony, Aesil, etc. are free. It has been found that the radical scavenging action is great to complete the present invention.

It is an object of the present invention to provide a free radical scavenging cosmetic of natural substances having a free radical scavenging action to prevent or delay skin aging, and other objects and advantages of the present invention are more clearly shown by the following examples and claims do.

The present invention provides a cosmetic composition for free radical scavenging containing a ginseng extract.

The present invention also provides a cosmetic composition for skin protection containing a ginseng extract.

Cyperus rotundus is a perennial herb that grows in Korea, China, and Japan. The leaves come from the rhizome, the flowers bloom in July-August, and the stems are 20-30cm long. -Three guns run and branches spread to mountain. Primarily roots, tubers attached to the tip of the beak contain essential oils and fatty oils. In oriental medicine, they are used for headache, abdominal pain, and menstrual irregularities.

Geumjeoncho (金錢草) refers to a medicinal herb made from the outpost of the Primrose of Lysimachia christinae (過路 黃). Geumchocho has a slight smell, tastes sweet and salty, and tastes cold. It has antipyretic and anti-inflammatory effects and is effective for kidney stones, cholelithiasis, cystitis and jaundice. The surface is brown or dark brown with vertical length and the lower part has beard root at the stem node. The leaves are opposite and mostly wrinkled, and when they are unfolded, they are wide egg-shaped or heart-shaped, and the petiole is slightly recessed and the edges are flat. This drug is green and leafy.

Hagocho (夏枯草, Prunella vulgaris ) is a perennial plant of the family Lamiaceae and is distributed in Korea, Japan, and Europe. The taste is very bitter, the nature is cold, acts on the liver, lowers heat, detoxifies, brightens the eyes. In folk medicine, decoction was used for inflammatory drugs including diseases of the mouth, tonsillitis. It was used for exudative and skin tuberculosis, adenopathy, swelling, flower bud, dermatitis and hair dandruff.

Lindera aggregate is an evergreen shrub or small arborescent of the camphor tree, which grows in southern China and Taiwan, and is a dicotyledonous plant that is also wild in the south of Japan. The leaves of the hibiscus are alternate, wide oval, with flat edges, and have three large veins. When young, it is covered with long hair, but soon disappears and the back is grayish white. Flowers bloom in March-April and form umbels in the axilla. Flowers are single flower, yellowish green, and flowers are divided into 6, and there are 9 stamens in male flower, 1 pistil and degenerated stamen in female flower. Fruits are nucleus, oval, mature from green to black. It is called Cheontae Oak because of its high quality. In the private sector, it was used for abdominal pain, dysmenorrhea and dyspepsia.

Lycopus lucidus refers to the ground before the flowering of the fly. It is named because it grows around ponds, and it is called Jisun because it can eat roots. This medicine is odorless, tastes very bitter, and has a mild warmth. It is mainly used for amenorrhea, menstrual pain, postpartum abdominal pain and bruise caused by fish blood, and is effective for boils and liver dysfunction. Appearance is node, outer surface is yellowish brown or purple, and there are species on each side, and the quality is light and easy to break.

Kwonsam (拳 蔘, Polygonum bistorta ) refers to the root stem of the tail tail with the ragweed . Kwonsam grows in the deep mountains and highlands of Korea, and in the spring and autumn, the stems are discarded and dried in the sun. This medicine is odorless, tastes bitter and cool. Ginseng is mainly used for dysentery due to moist heat, fecal bleeding, nosebleeds, traumatic bleeding, and diuretic swelling of the body, releasing convulsions due to fever, boils, sore throat, and snake bites. Pharmacological action has been reported to hemostatic and antibacterial action. The shape is bent in a flat columnar shape, and both ends are slightly pointed or tapered on one side and sometimes bent. The vagina is hard and the cut side is pale reddish brown or fuchsia.

Red Peony (赤芍 藥, Paeonia lactiflora ) is a plant of the family Acupuncture, 50-80cm tall, perennial. Flowers bloom in May-June, with white, red and pink lights. One large flower hangs at the end of the main stem, and 5 calyxes remain to the edge. Petals about 10, obovate, many stamens, yellow. Medicinal pain, fever, jingyeong, diuresis, hematopoiesis is effective.

Aesil (艾 實, Artemisia argyi ) is called Yellow Sea Mugwort and is a plant of Asteraceae. Its height is 45 ~ 120cm, perennial leaf is regenerated, thick, long lanceolate, and scented. Flowers are light brown in July-August and heads are arranged in cones at the ends of stems and branches. The fruit is a fruit. Diuresis for medicinal use. Swelling. It is effective in customs.

Hyangbuja, Geumchocho, Hagocho, Yakja, Taklan, Kwonsam, Red Peony, and Acyl extract contained in the cosmetic of the present invention are extracted by the conventional extraction method from the branches, leaves, roots, fruits, roots or starches of these plants. According to a preferred embodiment of the present invention, the hyangbuja root roots, geumchochoe forage, hachigogi fruit, five roots of the root, talanchoe forage, ginseng roots, red peony roots, acyl is extracted from the leaves.

The plant extract used in the cosmetic composition of the present invention may be obtained through various methods known in the art, and preferably comprises (a) water, (b) anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (e.g., Methanol, ethanol, propanol, butanol and the like), (c) acetone, (d) ethyl acetate, (e) chloroform or (f) 1,3-butylene glycol. More preferably, the extraction solvent is ethanol, most preferably about 70% ethanol. The plant extract, which is a material for extraction, may be used by drying the branch or leaves of the plant or as it is, and preferably by drying. As a drying method, natural drying or a hot air drying method can be used.

On the other hand, it will be apparent to those skilled in the art that the extract of the present invention can be obtained not only the above-described extraction solvent, but also plant extracts having substantially the same effect using other extraction solvents.

In addition, the extract of the present invention includes not only the extract by the above-mentioned extraction solvent, but also the extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also included in the plant extract of the present invention. The plant extracts of the present invention can be prepared in powder form by additional processes such as distillation under reduced pressure, freeze drying or spray drying.

The plant extract of the present invention has an activity of protecting the skin from skin damage caused by free radicals caused by external factors.

The external factors include ultraviolet rays, stress, and external harmful chemicals.These factors generate free radicals (eg, hydroxyl radicals, superoxide radicals, free radicals, and hydrogen peroxide) to increase oxidative stress in the skin, causing inflammation, Skin cell death and skin cell DNA damage occur, resulting in skin cancer or aging of the skin.

The term “skin damage” of the present invention means that the skin does not perform its normal function, and includes damage to skin components (such as collagen and elastin), impaired function of skin cells, and death of skin cells. .

The skin cells of the present invention include all cells present in the skin, preferably keratinocytes and fibroblasts.

The term “skin protection” of the present invention includes preventing damage to the skin and improving damaged skin.

The content of the plant extract of the present invention is 0.0001-20.0 wt% with respect to the total composition, preferably 0.01-10.0 wt%.

When the composition of the present invention is formulated as a cosmetic, the components included in the cosmetic composition of the present invention include components conventionally used in cosmetic compositions in addition to the plant extract as an active ingredient, such as antioxidants, stabilizers, solubilizers, vitamins Conventional adjuvants such as pigments and perfumes, and carriers.

The cosmetic composition for protecting the skin of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactants- Containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, sprays and the like, but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

As described in detail above, the present invention provides a cosmetic composition for free radical scavenging containing at least one extract selected from the group consisting of Hyangbuja, Geumchocho, Hagocho, Yakja, Taekran, Kwonsam, Red Peony, and Asil. The plant extract of the present invention can protect the skin from various skin damages occurring on the skin by eliminating free radicals in the skin generated by ultraviolet rays or the like.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, and the scope of the present invention is not limited by these examples in accordance with the gist of the present invention to those skilled in the art. Will be self-evident.

Example 1 Preparation of Plant Extract

To 1,000 g of each branch, leaf or root of Hyangbuja, Geumchocho, Hikcho, Yakyang, Taekran, Kwonsam, Red Peony, and Asil, 2,000 ml of 70% ethanol with the ratio of water and ethanol adjusted to 7: 3 was added for 3 days at room temperature. Leached. It was filtered and the filtrate was concentrated in vacuo to give a dry extract. The dried extract was dissolved in 30% 1,3-butylene glycol solution to a concentration of 1% (w / v), filtered and applied to the experiment of the present invention.

plant Scientific name Extraction site Peony Paeonia lactiflora Root Oak Lindera aggregate Root Hagocho Prunella vulgaris Isaac Talc Lycopus lucidus outpost Hyangbuja Cyperus rotundus near distance Geumjeoncho Lysimachia christinae outpost Ah Artemisia argyi leaf Kwonsam Polygonum bistorta near distance

Experimental Example 1: Free radical scavenging action

The free radical scavenging activity of the plant extracts prepared in Example 1 was measured as follows. That is, each plant extract was added to 100 ug / ml of each 60 ml of ethanol solution of 1,1-diphenyl-2-picryl-2-hydrazyl (DPPH). After reacting at room temperature for 30 minutes, absorbance was measured at 520 nm. Vitamin C was used as a comparative group. The free radical scavenging action was calculated by the following formula.

Free radical scavenging effect (%) = 100- (absorbance / control absorbance at extract treatment × 100)

The results are shown in Table 2 below.

Sample concentration (100ug / ml) Free radical scavenging effect (%) Peony 93.85 Oak 80.91 Hagocho 90.63 Talc 70.20 Flavor 60.38 Geumchocho (200ug / ml) 61.02 Ah 85.12 Kwonsam 94.65 Vit-C (100) 95.95

Through the above results, each plant extract was confirmed that the free radical scavenging effect is more than 60% at a concentration of 100ug / ml to 200ug / ml.

 Experimental Example 2: Inhibitory effect on hydrogen peroxide-induced cytotoxicity

The inhibitory effect on the hydrogen peroxide-induced cytotoxicity of the plant extract prepared in Example 1 was measured using mouse-derived fibroblasts.

Hydrogen peroxide generates hydroxyl radicals in cells, causing lipid peroxidation, leading to cell membrane damage and cytotoxicity. The free radical scavenging activity of each plant extract was measured as follows to test at the skin cell level. Human skin fibroblasts of the logarithmic growth stage were inoculated in a 96 well plate at 10,000 cells per well, incubated in 80 µl of DMEM medium for 24 hours, and then peroxidated in the medium so that the final concentration in the medium was 10-3M. 10 μl of hydrogen was added thereto, and the final concentration of the plant extract of Example 1 was added to 100 ug / ml. After 24 hours, 10 μl of 5 mg / ml MTT solution (Sigma, United States) was added, followed by 4 hours of dimethyl sulfoxide. The solution was added and dissolved in 200 μl per well, and then the absorbance was measured at 570 nm with a microplate reader.

In the same way, the survival rate of the cells treated only with plant extracts was also determined to determine whether the plants were cytotoxic.

Cell viability was calculated using the following formula.

Cell viability (%) = [1- (absorbance / control absorbance at extract treatment)] × 100

Sample concentration (100ug / ml) Cell viability after hydrogen peroxide treatment (%) Cell viability of the plant extract itself (%) Hydrogen peroxide treatment 0.0 - Peony 56.3 98.5 Oak 55.8 99.3 Hagocho 65.3 96.8 Talc 58.8 98.9 Flavor 69.7 99.3 Geumchocho (200ug / ml) 70.2 100.3 Ah 56.3 99.8 Kwonsam 63.5 97.9 Vit-C (100 ug / ml) 78.5 98.3

In the above results, each plant extract was found to have an excellent effect of inhibiting the cytotoxicity caused by hydrogen peroxide at 100ug / ml to 200ug / ml. Through the above results, it can be seen that the plant extracts of the present invention effectively eliminate free radicals in the skin caused by ultraviolet rays, environmental pollution, and the like.

Formulation examples are shown below, but the present patent is not limited to the following formulation examples. In addition, the following plant extract was used to dissolve the raw material of Example 1 in 50% 1,3-butylene glycol to 1% (w / v), respectively. Although only the plant extract is indicated in the following formulation example, it is a formulation example for each extract.

Formulation Example 1 Flexible Lotion (Skin)

Examples of the formulation of the flexible lotion (skin) in the cosmetic comprising the plant extract obtained above are as follows:

ingredient Content (% by weight) Plant extracts 0.5 1,3-butylene glycol 5.2 Oleyl alcohol 1.5 ethanol 3.2 Polysorbate 20 3.2 Benzophenone-9 2.0 Carboxyl Vinyl Polymer 1.0 glycerin 3.5 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 2: Milk Lotion

Formulation examples of the milk lotion in the cosmetic comprising the plant extract obtained above are as follows:

ingredient Content (% by weight) Plant extracts 0.6 glycerin 5.1 Propylene glycol 4.2 Tocopheryl Acetate 3.0 Liquid paraffin 4.6 Triethanolamine 1.0 Squalane 3.1 Macadamia Nut Oil 2.5 Polysorbate 60 1.6 Sorbitan sesquirrolate 1.6 Propylparaben 0.6 Carboxyl Vinyl Polymer 1.5 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 3: Nutrition Cream

An example of the formulation of the nutrient cream in the cosmetic containing the plant extract obtained above is as follows:

ingredient Content (% by weight) Plant extracts 1.0 glycerin 4.0 vaseline 3.5 Triethanolamine 2.1 Liquid paraffin 5.3 Squalane 3.0 Beeswax 2.6 Tocopheryl Acetate 5.4 Polysorbate 60 3.2 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 3.1 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 4: Massage Cream

An example of the formulation of the massage cream in the cosmetic containing the plant extract of the present invention is as follows:

ingredient Content (% by weight) Plant extracts 0.5 glycerin 4.0 vaseline 3.5 Triethanolamine 0.5 Liquid paraffin 24.0 Squalane 3.0 Beeswax 2.1 Tocopheryl Acetate 0.1 Polysorbate 60 2.4 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 2.3 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 5: Pack

Formulation examples of the pack in the cosmetic containing the plant extract obtained above are as follows:

ingredient Content (% by weight)  Plant extracts 1.0 Ethyl alcohol 3.0 EDTA-2Na 0.02 Propylene glycol 5.1 Glyceline 4.5 Cabopol 1.0 Polyoxide 0.1 antiseptic a very small amount incense a very small amount Purified water Remaining amount system 100

Claims (5)

A cosmetic composition for free radical scavenging containing Polygonum bistorta extract. A cosmetic composition for protecting skin containing Polygonum bistorta extract. The cosmetic composition according to claim 1 or 2, wherein the ginseng extract protects the skin by eliminating free radicals in the skin generated by ultraviolet rays. The cosmetic composition according to claim 1 or 2, wherein the content of the ginseng extract is 0.0001-20.0 wt% based on the total weight of the composition. The composition of claim 1, wherein the composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax Cosmetic composition, characterized in that it has a formulation selected from the group consisting of foundation and spray.