KR102414267B1 - For antioxidant, antidepressant and antistress compositions comprising citrus unshiu peel extract - Google Patents

Abstract

An object of the present invention is to provide a composition for antioxidants, antidepressants and antistresses based on natural products, which can exhibit not only an antioxidant effect while including a dermis extract but also a stress reducing effect and a depression prevention and amelioration effect. The present invention provides a composition that can be taken or used for a long time while being able to exhibit effects such as antioxidant, antidepressant, and anti-stress without the problem of side effects based on natural products.

Description

Antioxidant, antidepressant and antistress composition comprising a dermis extract

The present invention relates to an antioxidant, antidepressant and antistress composition comprising a dermis extract. More specifically, it is to provide a composition for antioxidant, antidepressant and antistress based on natural products that can exhibit not only an antioxidant effect while including a dermis extract but also a stress reducing effect and depression prevention and amelioration effect. In addition, the composition can be used as general food, functional food, functional flavoring, etc. with high palatability.

As modern society rapidly develops and diversifies, it demands various roles from modern people, and accordingly, the number of people complaining of generalized anxiety disorder and mental illness due to various stresses is increasing. According to the 「2016 Epidemiologic Survey of Mental Disorders」 released by the Ministry of Health and Welfare, the 'yearly prevalence of mental illness', which is the proportion of the population who experienced at least one mental illness in 2016, was 1 in 8 adults between the ages of 18 and 64. 12.1%. In addition, as of 2016, the 'lifetime prevalence of mental illness', which is the proportion of the population who experienced one or more mental disorders during their lifetime, was also found to be 26.6%, about one in four adults.

In addition, considering the recent trend of increasing mental illness among adolescents due to excessive academic enthusiasm or various kinds of stress, the prevalence rate of the entire population can be said to be even higher. Such stress is induced by pervasive factors in daily life and contributes to the occurrence of almost all psychiatric and medical diseases. Physiological reactions such as decrease in α-wave during EEG, increase in blood pressure and pulse rate, etc. occur. If these reactions are repeated, diseases such as depression, anxiety, and sleep disorders appear, causing personal, family and social loss, and cause lower quality of life. In this regard, compounds for the prevention or treatment of anxiety and depression have been proposed, but there is still a need for the development of substances that can effectively prevent, improve or treat anxiety, depression and stress using natural products with fewer side effects and safer. have.

Stress refers to the psychological and behavioral responses of each individual to adjust and adapt to internal and external pressures. In order to reduce the influence of real, symbolic and hypothetical stressors that threaten survival, the living body shows a series of reactions. The purpose of this reaction is to maintain homeostasis, which Claude Bernard calls the internal environment. Stressors disrupt the individual's equilibrium, and the individual responds to this by generating a physiological response to adapt and restore homeostasis. Stress causes changes in psychological homeostasis such as arousal level, memory, and emotion in the psychological aspect, and various physiological changes such as the endocrine, autonomic nervous system, and immune system related to the activation of the pituitary-adrenal axis in the biological aspect, and coping, motor system, etc. in the behavioral aspect. It causes a variety of adaptive behavioral changes. The stress response is basically an adaptive response for survival, but excessive activation can cause symptoms of anxiety disorders and depression. Therefore, in relation to the pathophysiology of stress-related diseases, research to explore the neurobiological mechanism of stress has been in progress for a long time.

Depression is an emotional pathology that occurs irrespective of the objective situation, in which the patient's entire life is covered with a depressed mood, a decrease in interest becomes anhedonia, It is a mental illness. In addition, susceptibility to diseases increases due to decreased appetite, insomnia, constipation, decreased libido, and decreased immune function, and various other physical symptoms are also exhibited. The strongest hypothesis in the art is that depression is caused by a lack of monoamine-based neurotransmitters serotonin, norepinephrin, and dopamine in the synapse of the central nervous system. is considered

Accordingly, most antidepressants have a pharmacological action that increases the concentration of neurotransmitters at serotonin or noradrenaline synapses. More specifically, antidepressants are largely tricyclic antidepressants (TCA), monoamine oxidase inhibitors (MAOIs), or selective serotonin reuptake depending on the mechanism by which they increase the concentration of neurotransmitters. inhibitors: SSRIs) are mainly used. Monoamine oxidase inhibitors such as phenelzine have not been used recently because they have a serious side effect of inducing heart disease. remains a problem. Therefore, recently, as an antidepressant with fewer side effects, the focus is on the development of an antidepressant using a selective serotonin (5-HT) reuptake inhibitor. , product name; Seroxat) and sertraline (product name; Zoloft) have been developed and their efficacy is widely recognized clinically.

However, these serotonin reuptake inhibitor-type drugs are only effective in about 60% of patients in the total administration group, and do not show therapeutic efficacy in the remaining 30-40% of patients, and serotonin spreading throughout the central nervous system Due to the widespread influence of serotonergic synapse, there is a problem accompanying unnecessary side effects depending on the patient to be administered. In addition, in the serotonergic drug response, it takes less than 1 hour to increase the level of serotonin at cerebral synapses, but a long-term drug of at least 3 weeks or longer until it shows therapeutic efficacy that actually improves the patient's mood (mood elevation) It has its limitations in that it needs to be taken.

KR 10-0513489 B1 KR 10-2013-0086459 A

It is an object of the present invention to provide a composition for antioxidant, antidepressant and antistress based on natural products that can exhibit not only an antioxidant effect while including a dermis extract but also a stress reducing effect and depression prevention and amelioration effect.

It is an object of the present invention to provide a composition that can be taken or used for a long time while being able to exhibit effects such as antioxidants, antidepressants, and antistresses without any side effects based on natural products.

It is an object of the present invention to provide a general food, health functional food, palatable fragrance, etc. comprising the composition.

In order to achieve the above object, the composition for antioxidant, antidepressant and antistress according to an embodiment of the present invention is to include a dermis extract.

In the antioxidant, antidepressant and anti-stress composition, the dermis extract may be extracted using an extraction solvent selected from the group consisting of water, C ₁ to C ₆ lower alcohols, and mixtures thereof.

The antioxidant, anti-depressant and anti-stress composition may further include a red pepper leaf extract.

The antioxidant, anti-depressant and anti-stress composition may be one that further comprises an extract of Beomaceae.

The antioxidant, antidepressant and antistress composition may further include a chazugi extract.

General food according to another embodiment of the present invention may include the antioxidant, anti-depressant and anti-stress composition.

Functional food according to another embodiment of the present invention may include the antioxidant, anti-depressant and anti-stress composition.

A fragrance according to another embodiment of the present invention may include the antioxidant, antidepressant and antistress composition.

The external preparation according to another embodiment of the present invention may include the antioxidant, antidepressant and antistress composition.

Hereinafter, the present invention will be described in more detail.

Antioxidant, antidepressant and antistress composition according to an embodiment of the present invention is to include a dermis extract.

Throughout this specification, 'extract' has the meaning commonly used as a crude extract in the sugar leaf system as described above, but in a broad sense also includes a fraction obtained by additionally fractionating the extract. That is, the extract includes not only those obtained using the above-described extraction solvent, but also those obtained by additionally applying a purification process thereto. For example, a fraction obtained by passing the extract through an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (prepared for separation according to size, charge, hydrophobicity or affinity), etc. The fraction obtained through the purification method is also included in the extract of the present invention.

Throughout this specification, when a part "includes" a certain component, it means that other components may be further included, rather than excluding other components, unless otherwise stated.

Throughout this specification, the term "combination of these" included in the expression of the Markush form means one or more mixtures or combinations selected from the group consisting of the components described in the expression of the Markush form, and the components It is meant to include one or more selected from the group consisting of.

Throughout this specification, the term "prevention" refers to any action that suppresses or delays the onset of arthritis by administration of the composition, and "treatment" or "improvement" means that the symptoms are improved or beneficial by administration of the composition Any action that changes

The extract used in the present invention may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze-drying or spray-drying.

"Antioxidant" as used in the present invention refers to exhibiting the effect of reducing active oxygen and inhibiting oxidation or aging of cells.

Depression as used in the present invention, also called depressive disorder, refers to a disease that causes a decrease in daily functioning by causing various cognitive, mental, or physical symptoms with a decrease in motivation and depression as the main symptoms. On the other hand, "anti-depression" as used in the present invention refers to the effect of being able to prevent and improve gastric depression or depressive disorder, and to improve motivation and depression.

The definition of stress in the present invention is slightly different for each scholar, but stress refers to a detrimental response or change to an organism caused by an external stimulus. In general, when the stimulus on the living body exceeds a certain level, it acts invasively. At this time, the organism responds to a certain threat regardless of the type of stimulus, such as tension headache, migraine, high blood pressure, indigestion, fatigue, pain, It causes hair growth and roughness of the skin, and if it continues chronically, it results in a nonspecific general adaptive syndrome, such as various neurosis and gastric ulcer, which is defined as stress (Selye, 1958, Longmans Green). and Co.). In particular, various diseases such as mental disorders such as depression, digestive disorders such as neurosis and duodenal ulcer, endocrine disorders such as stress hyperthyroidism, skin disorders such as stress spots or acne, and cardiovascular disorders such as arrhythmias or angina pectoris It has been reported that the disease is caused by stress.

"Anti-stress" as used in the present invention refers to the effect of preventing or ameliorating the aforementioned stress or diseases caused by stress.

The dermis (Citrus Unshiu Peel) means dried skins of mature fruits of Citrus unshiu Markovich. It is used for gastritis, indigestion, etc. as an aromatic dry placebo, and is used as an antitussive, antitussive, and expectorant.

When the dermis extract is included, it may exhibit an effect of preventing and alleviating stress and preventing and improving depression along with antioxidant activity. In addition, through the prevention and alleviation of stress, it is possible to prevent and improve various diseases that may be caused by stress.

In the composition for antioxidant, antidepressant and antistress, the dermis extract may be extracted using an extraction solvent selected from the group consisting of water, C ₁ to C ₆ lower alcohols, and mixtures thereof.

Preferably, the solvent may be any one selected from the group consisting of water, ethanol, and mixtures thereof. In the case of the solvent, the yield for the active ingredient exhibiting antioxidant, anti-stress and anti-depressant effects can be maintained at a high level, and thus a more excellent effect can be exhibited compared to a relatively small content.

The antioxidant, anti-depressant and anti-stress composition may further include a red pepper leaf extract.

The red pepper leaf refers to a leaf of hot pepper.

The antioxidant, anti-depressant and anti-stress composition may be one that further comprises an extract of Beomaceae.

The pan-tailed grass grows in sunny places in mountain valleys. 30 to 80 cm in height. The rhizome is short and thick, and there are many fine roots. The leaf attached to the root is alternate phyllotaxis, the petiole is long, broad egg-shaped, gradually narrows and has a sharp tip, and the bottom is in the shape of a heart. The edge of the leaf is flat and the back side is white. Leaves are 5 to 10 cm long and 3 to 7 cm wide. The leaves on the stem are similar to this, but the petiole is short and the leaves are small. The sheath is membranous (膜質: translucent like thin paper). Flowers bloom in June or July in light pink or white, and hang on the inflorescence. There are no petals and the sepals are divided into 5 pieces. There are 8 stamens, longer than the calyx, the anthers are light reddish purple, and there is a small gland [腺] at the bottom of the stamens. Fruits are aqueous and ripen in September or October, are wrapped in calyx, and have 3 ridges. The young leaves and stems are edible, and the rhizomes are used to relieve heat, control competition, and eliminate inflammation of boils. It is distributed in Korea, northeastern China, Heilong River, and Ussuri River.

The antioxidant, antidepressant and antistress composition may further include a chazugi extract.

The chazugi (Perilla frutescens var. acuta) has a straight stem, a height of 20 to 80 cm, a square cross section, a purple color, and a fragrance. The leaves are opposite, broad egg-shaped, pointed at the tip, rounded at the bottom, and serrated at the edge. There are hairs on both sides of the leaves, long hairs on the veins on the back side, and long petioles. Flowers bloom in August or September in light purple and run in raceme at the ends of stems and branches. The calyx has hairs and is split into two, and the upper part of the split pieces is split into three again, and the lower piece is split into two again. The corolla is short, cylindrical, and the tip is lip-shaped, and the lower lip is slightly longer than the upper lip. There are 4 stamens, and 2 of them are long. The fruit is a branch (separate: each fruit divided from the fissionaceae), is round, has a diameter of 1.5mm, and is contained in a calyx.

When the red pepper leaf, pantail grass and chazugi extract are further included, it may exhibit a synergistic effect on antioxidants, antidepressants and antistress.

Preferably, the antioxidant, antidepressant and anti-stress composition includes a dermis extract, and 0.1 to 20 parts by weight of a red pepper leaf extract based on 100 parts by weight of the dermis extract; It may be one comprising 5 to 50 parts by weight of sagebrush and 0.1 to 20 parts by weight of chazugi extract.

According to the above range, the antioxidant, antidepressant and anti-stress effects may be significantly increased due to the synergistic effect by the interaction of each extract. Therefore, it can produce a high effect with a smaller content, and there is no problem of cytotoxicity and other side effects even when taken for a long time.

More preferably, the antioxidant, antidepressant and anti-stress composition comprises a dermis extract, 0.1 to 20 parts by weight of a red pepper leaf extract based on 100 parts by weight of the dermis extract; 5 to 50 parts by weight of sagebrush; 0.1 to 20 parts by weight of chazugi extract; 0.1 to 10 parts by weight of Lamiaceae extract; It may include 0.1 to 10 parts by weight of red snow tea extract and 0.1 to 10 parts by weight of Cheonmundong extract.

The "Lamiaceae" is a perennial herb commonly grown in mountains and fields across the country, and is also distributed in China and Japan. There is a rhizome. Stems are reddish, hairy, and 20-60cm tall. The leaves are opposite, ovate or ovate oval, with a flat edge or some serrations. Flowers are densely hung on the inflorescence at the tip of the stem in May-July, and are purple, pink, white, and lip-shaped. The calyx is lip-shaped, divided into 5 parts. The lower lip of the corolla is divided into three parts. Stamens 4, 2 are long. The fruit is a soybean, split into 4, and ripens to yellowish brown.

The "Snow Tea" (Snow Tea, 紅雪茶) refers to a dried moss plant that grows wild in an old juniper tree in an alpine region.

The "Cheonmun-dong" grows on the seashore, and the rhizome is short and many cylindrical roots with pointed ends spread in all directions. The lower part of the stem has egg-shaped scales. Stems are green, reaching 1 to 2m in length, tendrils, and leaf-like branches run in groups of 1 to 3 and bend like a bow. Flowers bloom in May or June, hang 1 to 3 in leaf axils, light yellow, and small flower stalks have joints in the center and are similar to the length of petals. Inflorescences are yellowish-white in color and have a long oval shape. There are 6 petals and 6 stamens, and 3 styles. The fruit is a berry, round, about 6mm in diameter, matured in white, and contains 1 black seed.

When the Lamiaceae extract, red snow tea extract and Cheonmundong extract are further included in the above range, the interaction of each active ingredient not only exhibits an additional synergistic effect on antioxidant, antidepressant and antistress, but also significantly increases flavor and palatability. can pay Accordingly, it is possible to widely distribute food and fragrance compositions with excellent palatability.

General food according to another embodiment of the present invention may include the antioxidant, anti-depressant and anti-stress composition.

At this time, the amount of the extract in the food or beverage may be added in an amount of about 0.01 to 15% by weight of the total food weight, and the health drink composition may be added in a ratio of about 0.01 to 10g based on 100 ml, but is not limited thereto. .

When the food composition of the present invention is a beverage composition, there is no particular limitation on the other ingredients except for containing the extract as an essential ingredient in the indicated ratio, and it may contain various flavoring agents or natural carbohydrates as additional ingredients like conventional beverages. can Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; disaccharides such as maltose, sucrose and the like; and polysaccharides such as conventional sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (taumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) may be used, but are not limited thereto. The ratio of the natural carbohydrate may be generally in the range of about 1 to 20 g per 100 ml of the composition of the present invention, but may not be limited thereto.

In addition to the above, the composition of the present invention contains various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic flavoring agents and natural flavoring agents, coloring agents and thickeners (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its salts. salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonation agents used in carbonated beverages, etc., but may not be limited thereto.

In addition, the composition of the present invention may contain natural fruit juice and pulp for the production of fruit juice beverages and vegetable beverages, and these components may be used independently or in combination. The proportion of these additives is not critical, but may be selected from about 0.1 to about 20 parts by weight per 100 parts by weight of the composition of the present invention, but is not limited thereto.

Functional food according to another embodiment of the present invention may include the antioxidant, anti-depressant and anti-stress composition.

"Functional food" as used in the present invention is a food manufactured and processed by extracting, concentrating, refining, mixing, etc., a specific ingredient as a raw material for the purpose of health supplementation, or including health supplement food. In addition, it is a food designed and processed to sufficiently exert the biological control functions of the food ingredients, such as biological defense, regulation of biological rhythm, prevention and recovery of diseases, and functions related to disease prevention and recovery from diseases. It is assumed to include all that it has.

A fragrance according to another embodiment of the present invention may include the antioxidant, antidepressant and antistress composition.

When exemplifying the embodiment of the invention for the perfume, the composition of the present invention may be implemented in the form of a perfume, a diffuser, a scented candle, a massage oil, a body oil, a fragrance lamp, an aroma stick, a spray, a soap (eg, beauty soap) and a bath agent. can

In one embodiment, the composition of the present invention may be embodied as a perfume, a bath agent or a perfume formulation, in which case the perfume composition of the present invention may further include components commonly added to perfume manufacturing in addition to the mixed essential oil. These ingredients include fragrances, preservatives, stabilizers, colorants, antibacterial agents, deodorants, and disinfectants.

The external preparation according to another embodiment of the present invention may include the antioxidant, antidepressant and antistress composition.

The external preparation means that it is prepared in a form that can be applied to hair, skin, and the like. In addition, the external preparation in the present invention is not limited to the external preparation of quasi-drugs, and includes cosmetics. For example, it may be formulated as a body lotion, shampoo, hair conditioner, hair conditioner, hair treatment, antiperspirant, skin lotion, skin cream, deodorant, perfume (spray or fumigant), lipstick, lip cream, bath agent, etc. In particular, cosmetics such as skin lotions are included.

The present invention provides a composition for antioxidants, antidepressants and antistresses based on natural products that can exhibit not only an antioxidant effect while containing a dermis extract but also a stress reduction effect and a depression prevention and amelioration effect.

The present invention provides a composition that can be taken or used for a long time while being able to exhibit effects such as antioxidant, antidepressant, and anti-stress without the problem of side effects based on natural products.

The present invention provides a general food, a health functional food, a palatable fragrance, etc. comprising the composition.

1 relates to the antioxidant effect of a composition according to an embodiment of the present invention.
2 is related to the antioxidant effect of the composition according to an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in detail so that those of ordinary skill in the art can easily carry out the present invention. However, the present invention may be embodied in several different forms and is not limited to the embodiments described herein.

[Preparation Example: Preparation of plant extract]

1. Preparation of Dermal Extract

3g of the lyophilized dermis was put into 1mg of a 15ml tube, and 10ml of 70% ethanol was added again. The sample was put in a rounded mixed machine for 18 hours, mixed and extracted. After extraction, only the supernatant was collected after centrifugation at 3000 rpm for 10 minutes. Ethanol contained in the supernatant collected in this way was evaporated at a high temperature of 40 °C. And the remaining sample was freeze-dried. The dermis extract (CUE) used in this experiment was extracted with a yield of 8.4% from the raw material of the dermis.

2. Preparation of other plant extracts

Red pepper leaf extract (AE) was prepared by extracting dried and pulverized red pepper leaves with 70% ethanol (ethanol). In addition, beomtail extract (BE), chazugi extract (CE), lamifolia extract (DE), red snow tea extract (EE) and cheonmundong extract (FE) were also prepared in the same manner as above.

3. Preparation of Complex Extracts

In order to confirm the synergistic effect of mixing each extract, each extract was mixed according to the composition shown in Table 1 below to prepare a complex extract.

N1 N2 N3 N4 N5 N6 N7 N8 N9 CUE 100 100 100 100 100 100 100 100 100 AE 0.01 0.1 10 20 30 10 10 10 10 BE One 5 30 50 100 30 30 30 30 CE 0.01 0.1 10 20 30 10 10 10 10 DE - - - - - 0.01 0.1 10 20 EE - - - - - 0.01 0.1 10 20 FE - - - - - 0.01 0.1 10 20

(Unit: parts by weight)

[Experimental example: antioxidant, antidepressant, anti-stress test]

1. Animal Test Methods and Animal Groups

(1) Animal testing method

Thirty female 4-week-old Spraque-Dawley rats (Korea Central Animal, Gyeonggi-do, Korea), weighing 100 to 120 g, were purchased and bred at a temperature of 20 ± 2 °C and humidity of 55-60%. The day-night cycle was adjusted to 12 hours. There were no restrictions on general formula feed (PMI Nutrition LLC. Manufactured in the USA. 5L79) and drinking water, and the experiment was conducted after a period of adaptation to the breeding room environment for a week. All experiments were conducted during the day.

(2) group of animals

5 animals per group were divided into 3 groups. In group 1, water was administered orally as a control group, and in group 2, 15mg/150g (rat weigh) of the dermis was orally administered.

2. Antioxidant test

(1) Measurement of DPPH radical scavenging activity - Retained antioxidant in the dermis

DPPH (2,2-diphenyl-1-picrylhydrazyl)

The hydrazyl of DPPH (Diphenylpicryl hydrazyl) used in this experiment has the property of easily accepting hydrogen atoms because the nitrogen atom is in an unstable state. 1.9 ml of 0.2 mM DPPH solution (99.5% ethanol) was added to 0.1 ml (control: 99.5% ethanol) of a solution prepared in 5 concentrations of 0.05, 0.1, 0.2, 0.5, and 1 mg/ml of concentrated dry matter for each dermal material. After shaking for 10 seconds with a vortex mixer, it was incubated at 37 °C for 30 minutes. Then, the absorbance was measured at 517 nm using a spectrophotometer.

As a positive control drug, L-ascorbic acid was prepared at 5 concentrations of 0.05, 0.1, 0.5, and 1 mg/ml (99.5% ethanol) and measured. The antioxidant activity of each sample was expressed as % Antioxidant activity (electron donating ability) against DPPH.

The results are shown in [Fig. 1] below.

(2) Xanthine oxidase inhibitory activity measurement

In 0.1 ml of sample solution and 0.6 ml of 0.1 M potassium phosphate buffer of pH 7.5, 0.2 ml of substrate solution in which 2 mM xanthine was dissolved was added, 0.2 unit/ml xanthine oxidase 0.1ml was added, and reacted at 37°C for 5 minutes, followed by 1 N HCl 1 ml was added to terminate the reaction, and the absorbance of uric acid generated in the reaction solution was measured at 292 nm. Xanthin oxidase inhibitory activity was expressed as the absorbance reduction rate of the group with and without the sample solution.

Xanthine oxidase is an enzyme that acts as a rate-limiting enzyme in the terminal oxidation of all purines and as a source of oxidizing agents such as superoxide radicals and hydrogenperoxide. The inhibition of superoxide anion by the enzyme of xanthine/xanthine oxidase is indicated by the scavenging action of superoxide anion and inhibition of the xanthine oxidase enzyme, and has a biological significance by inhibiting the generation of free radicals. The superoxide radical scavenging effect of 50 μM allopurinol as a positive control was compared.

The results are shown in [Fig. 2] below. On the other hand, referring to [Fig. 2] below, it can be seen that the positive control group, allopurinol 50 μM, exhibits a higher scavenging effect of about 2 times or more at 1 mg/ml of the dermis.

3. Antidepressant behavior test (Forced Swimming Test, Forced Swimming Test, FST)

After the animals arrived at the kennel, they were acclimatized to the kennel environment for 14 days and acclimatized by conducting a forced swimming test for 15 minutes in all three groups to acclimatise to the water. After missing 5 minutes of the forced swimming test, it appears that they are actively trying to escape at the beginning, but they soon fall into a sleep fear and maintain an immobility according to the degree of depression, so immobility time was measured. The experiment was repeated 3 times.

On the other hand, the results are shown in [Table 2] below.

1st 2st 3st control 168.5 175.6 188.9 CUE 162.5 87.6 95.6

(Unit: seconds) Referring to [Table 2], it was confirmed that the immobility time was shortened when the dermis extract (CUE) was taken compared to the case where the water (Control) was taken orally.

4. Stress improvement effect test

Ten men and women who consented to this experiment were randomly classified into a control group taking a placebo and an experimental group taking dermis extract (CUE), each of which was arbitrarily divided into 5 people each, and consumed 400 mg each in the form of tea drinks for 4 weeks. In order to examine the psychological health improvement effect, PWI (Psychosocial Well-being Index), a social and psychological health measurement test, was conducted before and after intake. For objective comparison, the value of the control group was fixed at 10, the index was converted on a 10-point scale, and the index was evaluated as an index of 1 to 10, and the results are shown in [Table 3] below. In the following index, the lower the number, the better the stress improvement effect.

placebo CUE 10 8.0

(Unit: Index)

Referring to [Table 3], it can be seen that the continuous use of the dermis extract according to the present invention can help relieve stress. In particular, it can be seen that self-confidence and mental and physical stability can be increased according to the PWI index. Therefore, when the dermis extract is used continuously, it is possible to relieve stress, and it is possible to exhibit the prevention and improvement effect on diseases caused by stress.

4. For complex extracts Antioxidant, antidepressant, antistress test

Antioxidant (AO), antidepressant (AP), and antistress (AS) effects were evaluated for the complex extracts N1 to N9 in the same manner as above. In addition, in order to determine whether a synergistic effect appears compared to the dermis extract (CUE), the CUE was set as a fixed value, and the activities for N1 to N9 were compared.

Therefore, for objective comparison, the following evaluation indices fixed the CUE result to 1 in the case of AO and AP, and converted the values of N1 to N9 into indices of 1 to 10 by comparing them with the CUE. AO and AP in the above index, the higher the number, the better the effect. On the other hand, indices calculated as 1 and less than 1 in AO and AP are also denoted as 1. Therefore, the index indicated by 1 in AO and AP does not have a synergistic effect, or its effect is inferior to that of CUE alone.

In addition, in the case of AS, the CUE result is fixed at 10, and the values of N1 to N9 are compared with the CUE and converted into an exponent of 1 to 10. As for AS in the above index, the lower the number, the better the effect. On the other hand, the index calculated to exceed 10 by 1 in AS is also denoted as 10. Therefore, the index expressed as 10 in AS does not have a synergistic effect, or its effect is inferior to that of using CUE alone.

The results are shown in [Table 4] below.

CUE N1 N2 N3 N4 N5 N6 N7 N8 N9 AO One One 3.3 4.1 4.2 1.4 3.4 4.8 5.1 2.9 AP One One 2.4 3.1 2.9 One 2.9 4.7 5.1 1.1 AS 10 10 8.9 8.5 8.8 9.9 8.7 6.3 6.6 8.2

(Unit: Index)

Referring to [Table 4], in the case of N1, there was no synergistic effect on the complex extract, and in the case of N5, there was no significant synergistic effect while relatively increasing other extracts other than CUE, and it was confirmed that some indices were lower than CUE. could

However, as in N2 to N4, according to the embodiment in which the red pepper leaf extract, the sagebrush extract and the chazugi extract are included in a specific content range, compared to the case of using the dermis extract alone as a mutual combination of the active ingredients included in each extract It can be seen that there is a synergistic effect. In addition, in the case of N3, it was found that antioxidant activity, antidepressant activity and antistress activity were significantly improved.

On the other hand, it can be confirmed that in the case of N7 and N8, an additional synergistic effect can be exhibited. Therefore, it can be confirmed that, in the case of N7 and N8, very excellent antioxidant activity, antidepressant activity and anti-stress activity can be exhibited with a smaller content, and continuous administration or use for a long period of time is possible.

5. palatability test

The dermis extract (CUE) and N1 to N9 were processed into tea form, and each taster was asked to evaluate the preference based on flavor and taste. The tasters were asked to evaluate the palatability of N1 to N9 on an index of 1 to 10 by comparing the taste for the dermis extract by 10 people who evaluated the gastric stress relieving effect. The evaluated result values were taken as an average and are shown in [Table 5] below. The following evaluation values were rounded off with a decimal point, and the higher the number, the better the palatability.

CUE N1 N2 N3 N4 N5 N6 N7 N8 N9 flavor 5 5 4 4 4 2 4 8 8 7 taste 5 4 5 5 3 3 5 7 8 6

Referring to [Table 5], it can be seen that, in the case of N1 to N5, a similar or lower degree of palatability compared to the CUE appears. On the other hand, in the case of N7 to N9, it can be seen that the effect of improving the flavor and taste appears, and in particular, the degree of improvement in flavor and taste of N7 and N8 is excellent.

Therefore, it can be seen that, in the case of the above range, it can be provided as a food and flavoring composition with excellent palatability.

Although preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements by those skilled in the art using the basic concept of the present invention as defined in the following claims are also provided. is within the scope of the

Claims (9)

dermis extract;
Further comprising a red pepper leaf extract, sagebrush extract, chazugi extract, Lamiaceae extract, red snow tea extract and cheonmundong extract,
Based on 100 parts by weight of the dermis extract, 0.1 to 20 parts by weight of a red pepper leaf extract, 5 to 50 parts by weight of an oleracea extract, 0.1 to 20 parts by weight of a tea extract, 0.1 to 10 parts by weight of an extract of Lamiaceae, 0.1 to 10 parts by weight of a red snow tea extract As containing parts and 0.1 to 10 parts by weight of Cheonmundong extract,
Excellent antioxidant activity and stress-reducing effect
A composition for preventing or improving depression. The method of claim 1,
The extract is extracted using an extraction solvent selected from the group consisting of water, C ₁ to C ₆ lower alcohols and mixtures thereof.
Excellent antioxidant activity and stress-reducing effect
A composition for preventing or improving depression. delete delete delete A food composition comprising the composition according to any one of claims 1 to 2. A functional food for the prevention or improvement of depression comprising the composition according to any one of claims 1 to 2. A perfume composition comprising the composition according to any one of claims 1 to 2. An external preparation comprising the composition according to any one of claims 1 and 2.

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