KR102434150B1 - Compositions for inner beauty containing cirsium japonicum extract as effective component - Google Patents

Abstract

The present invention relates to an inner beauty composition comprising a milk thistle extract as an active ingredient. It is to provide a composition for inner beauty that can be usefully used in food and cosmetics made of inner beauty material that can exhibit various skin improvement effects without using it.

Description

Composition for inner beauty comprising milk thistle extract as an active ingredient {COMPOSITIONS FOR INNER BEAUTY CONTAINING CIRSIUM JAPONICUM EXTRACT AS EFFECTIVE COMPONENT}

The present invention relates to a composition for inner beauty comprising a milk thistle extract as an active ingredient. More specifically, it contains milk thistle extract as an active ingredient to show skin health improvement effects such as wrinkle improvement, whitening effect, antioxidant and anti-inflammatory effects. It relates to a composition for inner beauty of

Recently, in the domestic beauty market, various product lines have been introduced to reflect consumer needs and rapidly changing trends. In particular, today, when life is enriched and quality and value of life are prioritized, consumers' longing to pursue not only external beauty but also internal beauty has continued, and this has led to the establishment of a new concept of inner beauty in the beauty market.

In general, as a method to prevent skin damage, it is common to use functional cosmetics containing UV blockers or substances effective for wrinkle improvement, or to apply pharmaceuticals directly to the skin. Although effective in preventing dryness or moisturizing, these external preparations have a limitation in that the effect is temporary and only the applied area is effective.

Inner beauty is a compound word of inner and beauty, meaning inner beauty. At the same time, it implies to be satisfied.

In particular, increasing interest in well-being and LOHAS (Lifestyles of health and sustainability) due to the improvement of income level and changes in lifestyles of advanced countries, and by 2030, the elderly population will exceed 23%, contributing to a super-aging society As the number increases every year, the trend of consumers' preference for products for health and the desire to maintain beautiful skin and beauty is becoming more prominent.

Accordingly, the market for edible cosmetics (inner beauty) that improves the constitution of the skin, rather than cosmetics applied to the face for beauty, continues to grow. As products that break the stereotype that cosmetics are applied are released one after another, consumers are increasingly recognizing that they are a supplement to cosmetics that they eat or a product that is more effective for skin care. In particular, the skin beauty field included in the health functional food field is expected to have a greater marketability than other health functional foods (immunity, high blood pressure, diabetes, sexual function, etc.). development is also possible. It first appeared in Korea in 2002, about 10 years ago, and has been growing rapidly since 2010. With an annual growth rate of 15%, in 2013, it formed the president of about 20% of the health functional food market, or about 300 billion won. Materials used in these skin health functional foods can be broadly classified into vitamins, minerals, proteins and amino acids, unsaturated fatty acids, plant extracts, and other functional single substances, and these materials have respective skin-related functions and effects. .

The skin consists of the epidermis, including the cornified layer, which is the outer layer, the dermis, and the hypodermis. Although it helps only in the absorption of active ingredients, the Inner Beauty material containing functional substances is not used directly on the skin, but is consumed through the digestive system to create a healthy skin environment in the body. It can show moisturizing, wrinkle improvement and whitening effects by acting on the skin.

However, the trend that is consistent with the recent trend of inner beauty is that the function related to skin beauty is somewhat lacking compared to general cosmetics such as lotions and skins. It did not completely target the causative factors of skin-related diseases that occur through the pathway, and as a result, the inefficiency of skin care was revealed.

Therefore, the inventors of the present invention intensively studied a composition for inner beauty that is comparable or superior in functionality to general cosmetics, and as a result, it was confirmed through an experiment that the natural extract complex has superior skin functionality, and based on this, The present invention was completed.

KR 10-2019-0023439 A KR 10-2019-0016223 B1

It is an object of the present invention to contain milk thistle extract as an active ingredient to exhibit skin health improvement effects such as wrinkle improvement, whitening effect, antioxidant and anti-inflammatory effects, and an inner that can exhibit various skin improvement effects without directly using it on the skin It is to provide a composition for inner beauty for providing food of beauty material.

Another object of the present invention is to provide a cosmetic composition containing milk thistle extract as an active ingredient to improve wrinkles, whitening effects, antioxidant and anti-inflammatory effects.

The composition for inner beauty according to an embodiment of the present invention contains milk thistle (Cirsium japonicum) extract as an active ingredient, and exhibits wrinkle improvement, whitening effect, antioxidant and anti-inflammatory effects.

The milk thistle extract is extracted using an extraction solvent selected from the group consisting of water, alcohols having 1 to 6 carbon atoms, and mixtures thereof.

The composition comprises a fermented milk thistle extract fermented through a fermenting microorganism.

The fermenting microorganism is Lactobacillus casei (Lactobacillus casei), Lactobacillus rhamnosus (Lactobacillus rhamnosus), Bifidobacterium bifidum (Bifidobacterium bifidum) Bifidobacterium, Bifidobacterium breve (Bifidobacterium breve) It is selected from the group consisting of Lactobacillus acidophilus.

The composition is purified water, methanol (methanol), ethanol (ethanol), propanol (propanol), isopropanol (isopropanol), butanol (butanol), etc. containing 1 to 4 carbon atoms alcohol, acetone (acetone), ether (ether), The thistle extract fractionated with a solvent selected from the group consisting of benzene, chloroform, ethyl acetate, methylene chloride, hexane, cyclohexane, and mixtures thereof will include

Inner beauty food according to another embodiment of the present invention is prepared by including the composition for inner beauty.

A cosmetic composition according to another embodiment of the present invention is prepared by including the composition for inner beauty.

Hereinafter, the present invention will be described in more detail.

As used herein, the term "active ingredient" refers to a component that alone exhibits a desired activity or can exhibit activity together with a carrier that is inactive by itself.

As used herein, the term 'extract' has the meaning commonly used as a crude extract in the art as described above, but in a broad sense also includes a fraction obtained by additionally fractionating the extract. That is, the plant extract includes not only those obtained by using the above-described extraction solvent, but also those obtained by additionally applying a purification process thereto. For example, a fraction obtained by passing the extract through an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (prepared for separation according to size, charge, hydrophobicity or affinity), etc. Fractions obtained through various purification methods are also included in the plant extract of the present invention.

The extract used in the present invention may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze-drying or spray-drying.

The composition for inner beauty according to an embodiment of the present invention contains milk thistle (Cirsium japonicum) extract as an active ingredient, and exhibits wrinkle improvement, whitening effect, antioxidant and anti-inflammatory effects.

Thistle (Cirsium japonicum) is a perennial herb covered with hairs, slightly branched at the top, and grows upright, reaching 1m in height. There are large and small thorns on the edge of the leaf. Purple or red flowers bloom one by one at the end of a branch in midsummer. Each flower contains hundreds of small, barrel-shaped flowers. The fruit has hairs, and when it matures, it is blown away by the wind to reproduce. The young leaves are used as herbs, and the whole is dried and used for medicinal purposes. Similar plants include large thistle, fin thistle, and Korean thistle.

This milk thistle is called Daegye as a medicinal herb and is known to have anti-inflammatory and anti-inflammatory effects. In particular, milk thistle is known to have an excellent hemostatic effect, and the main component showing hemostasis is pectolinarin, which has been reported to reduce bleeding time in a dose-dependent manner in a mouse experiment. In addition, milk thistle promotes the proliferation of lymphocytes and at the same time increases the activity of NK cells and suppresses tumors, so it is considered to have anticancer and immune enhancing effects. It has been known to be used for the treatment of symptoms such as haematoma, bloody stool, or postpartum bleeding that does not stop, or symptoms such as dysphagia and boils.

As the thistle extract according to the present invention, one obtained by extraction and separation from nature using an extraction and separation method known in the art may be used. and, for example, it may include all of a hot water extract of milk thistle, a polar solvent-soluble extract, or a non-polar solvent-soluble extract.

The milk thistle extract is extracted using an extraction solvent selected from the group consisting of water, alcohols having 1 to 6 carbon atoms, and mixtures thereof.

Specifically, washing the natural product to prepare the extract; drying after washing; pulverizing the natural product after drying; leaching the pulverized product using an organic solvent; drying the sample after leaching; leaching with water; And including the step of leaching, it is possible to obtain a natural extract.

The natural extract extracted using the organic solvent may further include the step of performing fractionation using an organic solvent.

The method for preparing the extract may be a conventional extraction method in the art, such as an ultrasonic extraction method, a leaching method, and a reflux extraction method. Specifically, it may be an extract obtained by extracting a natural product from which foreign substances have been removed by washing and drying with water, an alcohol having 1 to 6 carbon atoms, or a mixed solvent thereof, and may be an extract extracted by sequentially applying the solvents to the sample.

The reflux extraction method is water, based on 100 mL of an alcohol having 1 to 6 carbon atoms, 10 to 30 g of a pulverized product of a natural product, a reflux time of 1 to 3 hours, and 50 to 100% alcohol or water having 1 to 6 carbon atoms. More specifically, based on 100 mL of an alcohol having 1 to 6 carbon atoms or 100 mL of water, 10 to 20 g of a pulverized product of a natural product, a reflux time of 1 to 2 hours, and 70 to 90% of an alcohol or water having 1 to 4 carbon atoms. .

The leaching method is carried out at 15 to 30° C. for 24 to 72 hours, and water or 50 to 100% alcohol having 1 to 6 carbon atoms is used as an extraction solvent. More specifically, the process is carried out at 20 to 25° C. for 30 to 54 hours, and the extraction solvent is water or 70 to 80% alcohol having 1 to 6 carbon atoms.

In the ultrasonic extraction method, the reaction is carried out at 30 to 50° C. for 0.5 to 2.5 hours, and the extraction solvent is water or 50 to 100% alcohol having 1 to 6 carbon atoms. Specifically, extraction is performed at 40 to 50° C. for 1 to 2.5 hours, and the extraction solvent is water or 70 to 80% alcohol having 1 to 6 carbon atoms.

The extraction solvent may be used in an amount of 2 to 50 times, more specifically, 2 to 20 times, based on the weight of the sample. For extraction, the sample may be left for 1 to 72 hours for extraction in the extraction solvent, and more specifically, for 24 to 48 hours.

After extraction, the extract may be fractionated by sequentially applying a fresh fractionation solvent. The fractionation solvent used for fractionation is any one or more selected from the group consisting of water, hexane, butanol, ethyl acetic acid, ethyl acetate, methylene chloride, and mixtures thereof, preferably ethyl acetate or methylene chloride.

After obtaining the extract or fraction, a method such as concentration or freeze-drying may be additionally used.

The composition comprises a fermented milk thistle extract fermented through a fermenting microorganism.

When the fermented milk thistle extract is used, there is an advantage that it can be used stably at a relatively high concentration due to low cytotoxicity, and the lowered palatability due to the peculiar odor of the natural product can be increased through fermentation.

The fermenting microorganism is Lactobacillus casei (Lactobacillus casei), Lactobacillus rhamnosus (Lactobacillus rhamnosus), Bifidobacterium bifidum (Bifidobacterium bifidum) Bifidobacterium, Bifidobacterium breve (Bifidobacterium breve) It is selected from the group consisting of Lactobacillus acidophilus.

The microorganisms used for the fermentation include Lactobacillus salivarius, Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus rhamnosus, Lactobacillus rhamnosus, Lactobacillus Lactobacillus plantarum), Lactobacillus helveticus, Lactobacillus fermentum, Lactobacillus paracasei, Lactobacillus casei, Lactobacillus casei, Lactobacillus casei, Lactobacillus Lactobacillus reuteri, Lactobacillus buchneri, Lactobacillus gasseri, Lactobacillus johonsonii, such as Lactobacillus johonsonii, Lactobacillus kefir, etc. Lactococcus lactis, Lactococcus plantarum, Lactococcus raffinolactis, Enterococcus faecalis, Enterococcus faecium, Enterococcus faecinium Lactic acid coxae and Bifidobacterium animals such as Streptococcus thermophilus, Leuconostoc lactis, Leuconostoc mesenteroides, etc., Bifidobacterium bifidium (Bifidobacterium bifidum), Bifidobacterium brev (Bifidobact) erium breve), Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium pseudolongum, Bifidobacterium themophilum, Bifidobacterium It may include bifidobacteria such as Bifidobacterium adolescentis, and more preferably Lactobacillus casei, Lactobacillus rhamnosus, Bifidobacterium bifidum) may be at least one selected from the group consisting of Bifidobacterium, Bifidobacterium breve, Bifidobacterium, and Lactobacillus acidophilus.

The composition is purified water, methanol (methanol), ethanol (ethanol), propanol (propanol), isopropanol (isopropanol), butanol (butanol), etc. containing 1 to 4 carbon atoms alcohol, acetone (acetone), ether (ether), Fermented thistle extract fractionated with a solvent selected from the group consisting of benzene, chloroform, ethyl acetate, methylene chloride, hexane, cyclohexane, and mixtures thereof will include

As a suitable solvent for extracting the extract from milk thistle, any solvent acceptable in the art may be used, and water or an organic solvent may be used. For example, purified water, methanol (methanol), ethanol (ethanol), propanol (propanol), isopropanol (isopropanol), alcohol having 1 to 6 carbon atoms including butanol (butanol), acetone (acetone), ether (ether) , benzene (benzene), chloroform (chloroform), ethyl acetate (ethyl acetate), methylene chloride (methylene chloride), hexane (hexane) and various solvents such as cyclohexane (cyclohexane) can be used alone or in combination, but Not limited.

As a method, any one of methods such as hot water extraction method, cold extraction method, reflux cooling extraction method, solvent extraction method, steam distillation method, ultrasonic extraction method, elution method, and compression method can be selected and used. In addition, the desired extract may be further subjected to a conventional fractionation process, and may be purified using a conventional purification method. There is no limitation on the method for preparing the milk thistle extract of the present invention, and any known method may be used.

Preferably, the milk thistle extract contained in the composition of the present invention is prepared in a powder state by an additional process such as vacuum distillation and freeze-drying or spray-drying the ethanol extract extracted by the solvent extraction method, and then further using the solvent. to obtain a purified fraction.

The composition further comprises an herbal extract selected from the group consisting of rosemary, lavender, thyme, sage, mint, carob, and mixtures thereof.

Herbal plants are herbaceous plants whose flowers, seeds, stems, leaves, and roots are useful for humans. Since ancient times, they have played an important role as medicinal herbs for pain relief and sedation, as well as for antiseptic and insecticidal properties. It is a natural material that is attracting more and more attention due to the toxicity of the material, concerns about environmental pollution, and its excellent efficacy.

Rosemary is a perennial evergreen deciduous tree in the family Lamiaceae, which usually grows over 1 meter. The narrow, thin needle-shaped leaves have a leather-like quality, are shiny, have a characteristic strong aroma, and are dark green. It has sterilization and disinfection action, so it was used to preserve meat, and it was often used in hospitals or when infectious diseases were prevalent. Rosemary helps to improve memory, revitalizes the symptoms of lethargy, and relieves headaches and migraines. In addition, it has an anti-obesity effect, regenerates the skin, revitalizes the sagging tissue, suppresses the occurrence of edema and dandruff, and promotes hair growth. Rosemary is used for cooking, facial cleansing, and to increase blood pressure when you have low blood pressure.

Lavender is one of the most well-known herbs. It is popular as an ornamental herb because the aromatic oil component makes the leaves and flower surfaces appear shiny, and their splendor shines even more during the flowering period. High-quality lavender is used for many purposes, especially flowers, leaves, stems, and the like, so it is suitable for ornamental purposes as well as potpourri and various cosmetic products. Dried flowers are dried in the morning by cutting the purple buds before flowering and hanging them in the shade with good ventilation. For ornamental use, cut off after the flowers have faded, leaving about 2 stalks. The lotion made with the essential oil of lavender is effective for rough skin because it has a cleansing effect that relaxes and relieves the skin and regenerates it clean and moist. Also, the scent of lavender has a calming effect, so it was put in a pillow and used for sleep. When you drink lavender tea, it has a calming effect, relieves pain and headaches, and helps you sleep by changing your mood.

Thym is an aromatic plant belonging to the family Lamiaceae and has small grayish-green leaves. There are two main varieties. One is the meridionale, also called serpolet. wild thyme) and the other cultivar (Thymus vulgaris), also called winter thyme (thym dhiver), German thyme (thym allemand), or farigoule, which is taller, has broader leaves and has a stronger bitter taste. . Lemon thyme (thym citron, T. citriodorus) is also widely used for its refreshing flavor in a variety of dishes. Thyme contains the essential oil thymol, which has a very fragrant and bactericidal effect. Thyme, one of the basic spice herbs in cooking, is raw or dried and used alone or added to making bouquet garni.

Sage has been used as a panacea since the Greek and Roman times. It is used in various ways such as ornamental, spice collection, medicine, cooking, dyeing, etc., and has tonic, soothing, digestive and sterilizing effects. Sage has many uses, but should not be used in large quantities at once. Sage tea is effective in clearing the mood, calming the excitement, and preventing stomatitis, bleeding gums, and bad breath. In addition, sage promotes digestion, detoxifies, placebo, and baths are used for fatigue recovery, expectoration, gout, diuresis, deworming, and antiseptic action. It is very effective for menopausal disorders.

Mint is a native of Europe, and along with lemon balm, it can be said to be a representative herb of the milwon plant. It is a perennial plant of the Lamiaceae family and one of the strongest herbs to grow. It has a strong mint scent that gives a refreshing and refreshing feeling just by rubbing the leaves. When storing, cut off the stems of the stems in June or July, when the scent is strongest, and dry them in the shade. Mint herbs, characterized by their refreshing feeling, are especially used to make sauces for meat dishes, and with their refreshing taste and scent, they are used to flavor beanies, carbonated water drinks, cocktails, etc. Although there are some differences depending on the type of mint, it is used as a raw material for sterilization, digestion promotion, gastric action, oral deodorant, toothpaste, and placebo. It has medicinal effects on dermatitis and itching, and when placed in the bathroom, the mint scent lasts for a long time instead of the bad smell, and it even has a strong sterilization effect. Green mint, also called spearmint, is often used in cooking along with peppermint. It has a sweeter flavor than peppermint and is very good for the skin.

Carob {Ceratonia siliqua (L.) Taub.} is an evergreen broad-leaved shrub growing to about 10 m in height and is drought tolerant. The leaves contain resin. Small, dull greenish-brown flowers bloom in autumn, with long green pods running. These pods turn chocolate when ripe and contain small, shiny hard beans. Carob gum, which softens the skin, is popular as a skin pack and is also used to treat diarrhea.

Preferably, the composition of the present invention is based on 100 parts by weight of the thistle extract, 40 to 60 parts by weight of rosemary extract, 40 to 60 parts by weight of lavender extract, 40 to 60 parts by weight of thyme extract, 40 to 60 parts by weight of sage extract, 40 parts by weight of mint extract. to 60 parts by weight and 40 to 60 parts by weight of carob extract.

When used as a complex extract within the above range, skin health improvement effects such as wrinkle improvement, whitening effect, antioxidant and anti-inflammatory effects can be increased due to the complex action between each component.

In addition, it is possible to provide an excellent food composition with no side effects as a component derived from aquatic products.

Preferably, the composition further comprises a natural extract selected from the group consisting of Aster fastigiatus Fisch. extract, Vicia pseudoorobus Fisch. & C.A.Mey., and mixtures thereof.

The Ongut namul (Aster fastigiatus Fisch.) is a perennial herb, distributed in Korea, China, Japan, and Russia. It is distributed all over Korea and grows to about 30-100cm in height. The stem is 30-100cm high, the stem is upright, there is a vertical ridge line, and the branch spreads from the upper part in a corymbly manner, and dense hairs are dense. Flowers bloom in August-October, 7-9mm in diameter, hanging on the corymbae at the end of the main stem, and the peduncle is 3-8mm long. Involucre is cylindrical, length 4mm, width 5mm, bract is arranged in 4 lines, lanceolate blunt head, with many hairs, and inner part is 1.5mm long. Snowflakes are white in one row and corolla is 5-6.5mm long and 1mm wide. The first leaf remains as it is until the flower blooms, linear lanceolate, narrow at both ends, 5-12cm long, 4-15mm wide, the lower part narrows into a petiole, and the back side turns white, with dot and double hairs. It has sparse sawtooths on the edge, often curled backwards, and short hairs on the upper edge. Cauline leaves gradually get smaller as they go up, and are linear lanceolate or linear, and the back side has dense double hairs, dotted, and inflorescence leaves are 2-3mm long.

The large raker (Vicia pseudoorobus Fisch. & C.A.Mey.) is a perennial vine, and has a stem of 80-150 cm in length with or without hairs. The leaf is an upper right ventral leaf composed of 2-5 pairs of leaflets, and the tendril at the tip is not split or split. Leaflets are ovate, acute or obtuse, 3~5cm long and 15~30mm wide, turn yellowish-brown when dry, veins on the back side protrude, and stipules are green and split sharply. The flowers come out from the leaf axils, are 4-8cm long, and the flower stalks are long and slanted from the center to one side, and many flowers run in a raceme. Flowers bloom in September, 13-15mm long, purple, and the sepals are low triangular. The leaf is an upper right ventral leaf composed of 2-5 pairs of silver leaflets, and the tendril at the tip is not split or split. Leaflets are ovate, acute or obtuse, 3-5cm long and 15-30mm wide, turn yellowish-brown when dried, and the leaf veins on the back side are protruding, and the stipules are green and split sharply.

When the composition further includes a natural extract selected from the group consisting of an ongut extract, a hornethorn extract, and a mixture thereof, in addition to the thistle extract and herbal extract, as it is composed of a complex extract, only thistle extract is used as a mixture Compared to the case where the skin health is improved, the effect of improving skin health can be increased.

Preferably, the composition of the present invention is based on 100 parts by weight of the thistle extract, 40 to 60 parts by weight of rosemary extract, 40 to 60 parts by weight of lavender extract, 40 to 60 parts by weight of thyme extract, 40 to 60 parts by weight of sage extract, 40 parts by weight of mint extract. to 60 parts by weight, 40 to 60 parts by weight of the carob extract, 5 to 10 parts by weight of the ongut namul extract, 5 to 10 parts by weight of the black rake extract and 5 to 10 parts by weight of the primrose extract.

When used as a complex extract within the above range, skin health improvement effects such as wrinkle improvement, whitening effect, antioxidant and anti-inflammatory effect are further increased due to the complex action between each component.

Inner view food according to another embodiment of the present invention is prepared by including the composition for inner beauty as an active ingredient.

The composition for inner beauty may be added as it is or used together with other foods or food ingredients, and may be appropriately used according to a conventional method. The mixing amount of the active ingredient may be suitably determined according to its intended use (prophylactic, health or therapeutic treatment). However, in the case of long-term intake for health and hygiene or health control, the amount may be below the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range. .

There is no particular limitation on the type of the food. Examples of foods to which the composition can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, various soups, beverages, tea, drinks, There are alcoholic beverages, vitamin complexes, and the like, and includes all health foods in the ordinary sense.

The health beverage composition of the present invention may contain various flavoring agents or natural carbohydrates as additional ingredients, as in a conventional beverage. The above-mentioned natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. As the sweetener, natural sweeteners such as taumarin and stevia extract, synthetic sweeteners such as saccharin and aspartame, etc. can be used.

In addition to the above, the health beverage composition of the present invention includes various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, It may contain alcohol, a carbonation agent used in carbonated beverages, and the like. In addition, the health beverage composition of the present invention may contain fruit for the production of natural fruit juice, fruit juice beverage, and vegetable beverage. These components may be used independently or in combination.

A cosmetic composition according to another embodiment of the present invention is prepared by including the composition for inner beauty as an active ingredient.

The dermatologically acceptable carrier includes purified water, oil, wax, fatty acid, fatty acid alcohol, fatty acid ester, surfactant, moisture absorbent, thickener, antioxidant, viscosity stabilizer, chelating agent, buffer, preservative, lower alcohol, etc. However, the present invention is not limited thereto, and the types and concentrations thereof are various and include parts that those skilled in the art can change within the scope of the present invention.

Cosmetics prepared from the cosmetic composition of the present invention can be prepared in the form of general emulsified formulations and solubilized formulations. Cosmetics with emulsified formulations include nutritional lotions, creams, and essences, and solubilized cosmetics include softened lotions.

The cosmetic may be prepared in the form of an adjuvant that can be applied topically or systemically commonly used in the art by containing a dermatologically acceptable medium or base in addition to the extract of the present invention.

Suitable cosmetic formulations include, for example, solutions, gels, solid or kneaded dry products, emulsions obtained by dispersing an oil phase in an aqueous phase, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes), non-ionic It may be provided in the form of a vesicular dispersant, cream, skin, lotion, powder, ointment, spray or in the form of a cone stick. In addition, it may be prepared in the form of a foam or an aerosol composition further containing a compressed propellant.

Products to which the cosmetic composition of the present invention can be added include, for example, astringent lotion, softening lotion, nutritional lotion, various creams, essence, pack, foundation, etc. etc. Specific formulations include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, essence, nourishing essence, pack, soap, shampoo, cleansing foam. , cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder, loose powder or eye shadow.

According to the composition for inner beauty comprising the milk thistle extract of the present invention as an active ingredient, it contains milk thistle extract as an active ingredient to show skin health improvement effects such as wrinkle improvement, whitening effect, antioxidant and anti-inflammatory effects, and is used directly on the skin It is possible to provide a composition for inner beauty of a food material that can exhibit various skin improvement effects without doing so.

1 is a graph measuring the cell viability by the composition for inner beauty according to an embodiment of the present invention.
Figure 2 is a graph measuring the skin wrinkle improvement effect by the composition for inner beauty according to an embodiment of the present invention.
3 is a graph measuring the skin whitening effect of the composition for inner beauty according to an embodiment of the present invention.
4 is a graph measuring the antioxidant and anti-inflammatory effects of the composition for inner beauty according to an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in detail so that those of ordinary skill in the art can easily carry out the present invention. However, the present invention may be embodied in several different forms and is not limited to the embodiments described herein.

[Preparation Example 1: Preparation of thistle extract]

1. Preparation of thistle extract

First, the thistle was washed, dried, and then pulverized. The pulverized product was mixed with ethanol, kept at 98 to 100° C. for 2 hours, cooled and filtered with Whatman filter paper to obtain the filtrate, which was prepared as an ethanol extract of milk thistle (JE).

2. Preparation of Fermented Thistle Extract

Among microorganisms using 10% (w/w) of the milk thistle ethanol extract (JE) for fermentation, Streptococcus thermophilus was added with 75% (w/w) D.W, 15% (w/w) Fermented thistle extract (SJE) was prepared by inoculating the mixed sterile medium and then culturing (25° C., 24 hours).

3. Preparation of thistle fraction

The thistle ethanol extract (JE) was suspended in 2L of distilled water, fractionated with an equal amount of methylene chloride, and then concentrated under reduced pressure to obtain a milk thistle fraction (JME).

4. Preparation of Fermented Thistle Fraction

The fermented thistle extract (SJE) was suspended in 2L of distilled water, fractionated with an equal amount of methylene chloride, and concentrated under reduced pressure to obtain a fermented thistle fraction (SJME).

[Preparation Example 2: Preparation of complex extract]

1. Preparation of herbal extracts

First, the rosemary was thoroughly washed under running water and then completely air-dried. The dried rosemary was pulverized with a blender and then prepared into powder. After adding 50% ethanol as an extraction solvent to the powder sample at a ratio of 1;10 (w; v), the powder sample was completely immersed, and extracted three times for 3 hours while refluxing at 80°C. The extract is Whatman No. 2 filtered with filter paper. The filtrate was concentrated under reduced pressure at 60° C. to prepare a rosemary extract (RH).

Lavender extract (LE), thyme extract (TE), sage extract (SE), mint extract (ME), and carob extract (KE) were prepared using the same method as the preparation method of the rosemary extract (RH).

3. Preparation of other extracts

By using the same method as the preparation method of the milk thistle ethanol extract (JE) prepared in Preparation Example 1, an ong sprout extract (AE) and an extract (VE) were prepared.

4. Preparation of Complex Extracts

Thistle ethanol extract (JE), fermented thistle extract (SJE), milk thistle fraction (JME), fermented thistle fraction (SJME), rosemary extract (RH), lavender extract (LE), sage extract (SE) prepared in Preparation Example 1 ), mint extract (ME), carob extract (KE), ongut sprouts extract (AE), and black rake extract (VE) were mixed in the content range as shown in Table 1 below to prepare a complex extract.

IB1 IB2 IB3 IB4 IB5 IB6 IB7 IB8 IB9 IB10 BI11 IB12 IB13 IB14 IB15 JE 100 - - - 100 100 100 100 100 100 100 100 - - - SJE - 100 - - - - - - - - - 100 - - JME - - 100 - - - - - - - - - - 100 - SJME - - - 100 - - - - - - - - - - 100 RE - - - - 20 40 60 80 60 60 60 60 60 60 60 LE - - - - 20 40 60 80 60 60 60 60 60 60 60 SE - - - - 20 40 60 80 60 60 60 60 60 60 60 ME - - - - 20 40 60 80 60 60 60 60 60 60 60 KE - - - - 20 40 60 80 60 60 60 60 60 60 60 AE - - - - - - - - 3 5 10 13 10 10 10 VE - - - - - - - - 3 5 10 13 10 10 10

(Unit: parts by weight)

[Experimental Example 1: Measurement of cell viability]

cell culture

RAW 264.7 cell, a mouse macrophage cell line, was purchased from the Korea Cell Line Bank (KCLB) and used. The RAW 264.7 cells were cultured in an incubator controlled at 37° C. and 5% CO ₂ using DMEM medium containing 10% FBS and 1% penicillin-streptomycin.

Cell viability measurement

RAW 264.7 cells were aliquoted in a 96-well plate at a concentration of 1X10 ⁴ cells/well and cultured for 24 hours, and the samples were treated with each concentration (50, 150, 200 ug/mL). As a positive control, 1 ug/mL of lipopolysaccharide (LPS) was treated, and after 24 hours of incubation, CCK-8 solution (10 mL/well) was added to each well and treated at 37°C for 2 hours to 450 Absorbance was measured at nm wavelength using an ELISA reader (TECAN, Mnnedorf, Switzerland).

The results are shown in FIG. 1, and as shown in FIG. 1, the complex extracts IB1 to IB15 prepared in Preparation Example 2 showed a survival rate of 80% or more at all concentrations compared to the positive control, so it was confirmed that there was no cytotoxicity. .

[Experimental Example 2: Wrinkle improvement effect of milk thistle extract]

In order to evaluate the anti-wrinkle effect of the milk thistle extract (JE, SJE, JME and SJME) of the present invention, the elastase inhibitory activity was confirmed.

Elastase is an enzyme that decomposes elastin, which plays a very important role in the elasticity of the dermal layer of the skin. To 220 μl of the sample, 20 μl of 2 mg/ml N-succinyl-Ala-Ala-Ala-p-nitroanilide was added and reacted at 25° C. for 10 minutes, followed by 20 μl of 1 μg/ml porcine pancreatic elastase (PPE). was added and reacted for 20 minutes at 25°C.

After terminating the reaction on ice, absorbance was measured at 405 nm using a microplate reader.

At this time, adenosine, a skin elasticity component, was used as a positive control group, and the experimental results for the inhibitory ability of elastase were found as the absorbance reduction rate of the milk thistle extract (JE, SJE, JME and SJME) treated group and the untreated group. indicated.

The results are shown in FIG. 2 below, and the treatment of milk thistle extract (JE, SJE, JME and SJME) showed high inhibition of elastase activity even when compared to adenosine, a positive control. , It was confirmed that the milk thistle extract (JE, SJE, JME and SJME) according to the present invention is effective in improving wrinkles.

In particular, in the case of the fermented milk thistle fraction (SJME) among the milk thistle extracts (JE, SJE, JME and SJME) of the present invention, a high ability to inhibit Elastase activity was observed, and a concentration-dependent inhibitory ability of Elastase activity was confirmed.

[Experimental Example 3: Whitening effect of milk thistle extract]

In order to evaluate the whitening effect of the milk thistle extract (JE, SJE, JME and SJME) of the present invention, the tyrosinase inhibitory activity was confirmed.

Tyrosinase (Tyrosinase) is an enzyme that regulates the production of skin melanin, it can inhibit melanin production through tyrosinase inhibitory activity. In the experiment to confirm the whitening effect, 50 μl of the sample and 50 μl of 100unit/ml Tyrosinase were added to 50 μl of 0.1M phosphate buffer (pH 6.5) and reacted at room temperature for 5 minutes. 100 μl of 1 mM L-DOPA was added and reacted at 37° C. for 30 minutes, followed by measuring the absorbance at 475 nm using a microplate reader.

At this time, the tyrosinase inhibitory activity was expressed as the absorbance reduction rate of the milk thistle extract (JE, SJE, JME and SJME) treated group and the untreated group.

The results are shown in Figure 3 below, and the treatment of the milk thistle extract (JE, SJE, JME and SJME) showed a high ability to inhibit tyrosinase activity even when compared to adenosine, a positive control. , It was confirmed that the milk thistle extract (JE, SJE, JME and SJME) according to the present invention is effective in skin whitening.

In particular, in the case of the fermented milk thistle fraction (SJME) among the milk thistle extracts (JE, SJE, JME and SJME) of the present invention, a high tyrosinase activity inhibitory ability was observed, and a concentration-dependent tyrosinase activity inhibitory activity was also observed.

[Experimental Example 4: Antioxidant and anti-inflammatory effect of milk thistle extract]

In order to evaluate the antioxidant and anti-inflammatory effects of thistle extracts (JE, SJE, JME and SJME) of the present invention, the NO production inhibitory ability was confirmed.

In the experiment to confirm the anti-inflammatory effect, RAW 264.7 cells (murine macrophages) were aliquoted at 2×10 ⁴ cells/well in 1.96 well plate, and then incubated for 12 hours at 37° C., 5% CO ₂ in an incubator to completely attach the cells. and LPS, which is an inflammatory substance, at a concentration of 1 μg/ml at the same time as the sample treatment was co-treated for 24 hours, and then the supernatant was separated.

Next, the NO content was measured in the separated culture supernatant, and the NO content was 517 nm using a microplate reader after treating 100 μl of the separated culture supernatant with the same amount of Griess (Sigma) reagent and reacting for 10 minutes. absorbance was measured.

At this time, the concentration of nitric oxide was calculated by comparing with a standard straight line obtained using sodium nitrite (NaNO ₂ , Sigma).

The results are shown in Figure 4 below, and when LPS, which is an inflammatory substance, was treated, the secretion of NO, an inflammatory marker, was increased in the sample untreated group, and the treatment of thistle extract (JE, SJE, JME and SJME) had the ability to inhibit NO production. was confirmed to increase.

In particular, in the case of the fermented milk thistle fraction (SJME) among the thistle extracts (JE, SJE, JME and SJME) of the present invention, the NO production inhibitory ability was observed to be high, and the concentration-dependent NO production inhibitory ability was confirmed.

[Experimental Example 5: Effect of complex extract]

In order to confirm the effect on the composition for inner beauty of the present invention, the complex extracts (IB1 to IB15) prepared in Preparation Example 2 were tested in the same manner as in Experimental Examples 1 to 4 to determine the effect of the complex extract. Confirmed.

For comparison, the fermented milk thistle fractions (IB4, SJME) showing excellent effects were placed as index 1, and are shown in Table 2 below.

IB4, SJME IB5 IB6 IB7 IB8 IB9 IB10 IB11 IB12 IB13 IB14 IB15 Skin wrinkle improvement effect One 3 5 5 4 4 5 6 5 7 7 9 skin whitening effect One 3 4 5 4 5 6 7 5 8 8 9 Skin antioxidant and anti-inflammatory effect One 3 5 5 5 4 6 6 5 7 8 9

(Unit: Index)

As shown in Table 2 above, in the case of the complex extract (IB15) containing the fermented milk thistle fraction (SJME), the complex extract containing the milk thistle ethanol extract (JE), the fermented thistle extract (SJE), and the thistle fraction (JME) Compared to IB1 to IB14, it was confirmed that the skin wrinkle, skin whitening, antioxidant and anti-inflammatory effects of equal or higher were exhibited.

[Experimental Example 6: Evaluation of palatability]

The palatability evaluation was performed for IB1 to IB15 of the present invention. After preparing IB1 to IB15 as an inner beauty drink, it was provided to 20 adult men and women, and evaluation of taste, aroma and overall preference was requested.

The evaluation score was requested to be evaluated on a scale of 1 to 10 points for each item, and the evaluation result was converted into an average score. In the index, the higher the number, the higher the degree of preference.

IB1 IB2 IB3 IB4 IB5 IB6 IB7 IB8 IB9 IB10 BI11 IB12 IB13 IB14 IB15 taste One 3 3 4 5 7 7 4 6 7 8 7 8 8 9 incense 2 4 3 4 5 4 6 5 6 7 7 6 8 9 9 Overall preference (average) 1.5 3.5 3.0 4.0 5.0 5.5 6.5 4.5 6.0 7.0 7.5 6.5 7.5 8.5 9.0

(Unit: Index)

Referring to Table 3, it was confirmed that in IB1 to IB4, the preference was lowered due to the unique taste and aroma of milk thistle. However, when using a mixture of other natural extracts of IB5 to IB12, it was confirmed that the palatability is excellent.

In particular, in the case of the complex extract IB15 containing the fermented milk thistle fraction, it was confirmed that it exhibited relatively excellent taste and aroma, and it was confirmed that it was preferred by the evaluators. Therefore, it was confirmed that the inner beauty beverage prepared with the composition for inner beauty of the present invention was significantly improved in terms of functionality as well as palatability.

Although preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements by those skilled in the art using the basic concept of the present invention as defined in the following claims are also provided. is within the scope of the

Claims (7)

Contains a fermented milk thistle (Cirsium japonicum) fraction as an active ingredient,
The fermentation is fermented through the fermenting microorganism Streptococcus thermophilus,
rosemary extract, lavender extract, sage extract, mint extract, carob extract, Aster fastigiatus Fisch. extract, and Vicia pseudoorobus Fisch. & CAMey. extract;
Based on 100 parts by weight of the fermented milk thistle fraction, 40 to 60 parts by weight of rosemary extract, 40 to 60 parts by weight of lavender extract, 40 to 60 parts by weight of sage extract, 40 to 60 parts by weight of mint extract, 40 to 60 parts by weight of carob extract, To be included in 5 to 10 parts by weight of Ongut namul extract, 5 to 10 parts by weight of extract,
Wrinkle improvement, whitening effect, antioxidant and anti-inflammatory effect
Composition for inner beauty. delete delete delete delete A composition comprising the composition for inner beauty according to claim 1
Inner beauty food. A composition comprising the composition for inner beauty according to claim 1
cosmetic composition.

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