KR102124065B1 - Composition for improving skin wrinkle comprising plant extract - Google Patents

Abstract

The present invention relates to a composition for improving skin wrinkles, and more specifically, two or more plant extracts selected from the group consisting of white pattern thistle extract, ginger extract, European equestrian extract, licorice extract and red shamrock extract as an active ingredient It relates to a composition for improving skin wrinkles. The composition of the present invention significantly increases the length of the chains of the glycosaminoglycans bound to them and the expression of decorin and biglycan, which are the major proteins constituting the dermis of the skin, thereby improving elasticity and improving wrinkles. As it has, it can be usefully used as a composition for improving skin wrinkles and elasticity.

Description

Composition for improving skin wrinkles containing plant extracts {COMPOSITION FOR IMPROVING SKIN WRINKLE COMPRISING PLANT EXTRACT}

The present invention relates to a composition for improving skin wrinkles, and more particularly, to increase or express the expression of decorin and biglycan, the matrix proteins of the dermis, of glycosaminoglycans It relates to a composition for improving skin wrinkles containing a plant extract that can improve skin wrinkles and improve elasticity by increasing the length of the chain as an active ingredient.

The skin is the widest body tissue, and is the organ that primarily contacts the external environment and irritation. It acts as the first defense against foreign substances and irritation, as well as metabolism in the body that regulates body temperature and moisture in the body. It plays an important role. In addition, since the skin is the first organ seen externally, it is also a very important organ expressing external beauty. This external beauty tends to act in a similar way to health in modern society.

As the number of older people increases, the beauty of appearance becomes the standard of judgment that symbolizes youth and health. In addition, for young people, this external beauty is revealed with confidence and as a competitive power that affects one's work ability. Because it is located. For this reason, efforts are being actively made to suppress skin aging and skin wrinkles from the academic and industrial aspects.

The main external change in skin aging is a decrease in wrinkles and skin elasticity, which is known to be due to structural changes in the dermis. The dermis is the structure of the skin that lies beneath the outermost epidermis of the skin, and collagen and elastic fibers make up the most weight. These collagen and elastic fibers play an important role in maintaining skin elasticity. The dermis of histologically aged skin has a reduced collagen fiber thickness, a loose array, and a complex and elaborate bond between elastic fibers. This phenomenon is due to the expression of collagen and elastic fiber degrading enzymes due to continuous exposure to ultraviolet rays, and is the main cause of wrinkle formation in the skin of the elderly (Patent Document 1).

The development of functional cosmetics to improve skin wrinkles has been centered on the inhibition of enzymes such as matrix metalloproteinase (MMP)-1 that promotes the breakdown of collagen and the increased expression of collagen, a key matrix protein, but has a substantially satisfactory effect. The development of visible functional cosmetics is still far away. Ingredients such as retinoic acid or retinol are known to have effective effects, but strong side effects appear in the case of retinoic acid, relatively low side effects in retinol, but also side effects may occur, and stability is weak, so activity is easy. It has various problems such as weakening.

The MMP is a factor having a decisive effect on collagen decay in the dermal layer, and many studies have been made on improving skin wrinkles through suppression of MMP expression or activity. Activated MMP is regulated by an inhibitor such as 2-Macroglobulin or TIMP (Tissue Inhibitors of Metalloproteinase), and many cells, including keratinocytes and fibroblasts of the skin, secrete MMP ( Non-Patent Document 1). MMP activity plays a very important role in the formation of wrinkles on the skin because the MMP activity in the skin is increased and the collagen in the skin is remarkably collapsed even after only one ultraviolet irradiation. However, few studies have been conducted on improving skin wrinkles through increased expression of dermal proteins other than collagen.

Republic of Korea Patent Publication No. 10-2017-0037570

Fisher G.J. et al., Photochem. Photobiol., 69, 154, 1999

An object of the present invention is to provide a composition for improving skin wrinkles containing a plant extract having an effect of improving skin elasticity and an effect of improving skin wrinkles through an activity of increasing decolin and biglycan expression.

In order to achieve the above object, the present invention is to improve skin wrinkles containing two or more plant extracts selected from the group consisting of white pattern thistle extract, ginger extract, European equestrian extract, licorice extract and red shamrock extract. Provided is a cosmetic composition.

According to one embodiment of the invention, the extraction solvent used for the preparation of the plant extract may be water, an organic solvent or a mixture thereof, the organic solvent is alcohol, hexane, ether, glycerol, propylene glycol, butylene glycol , Ethyl acetate, methyl acetate, dichloromethane, chloroform, benzene, or a mixed solvent thereof, but is not limited thereto. According to a preferred embodiment of the present invention, the alcohol may be a lower alcohol, such as methanol, ethanol, propanol or butanol, but is not limited thereto.

According to one embodiment of the present invention, the cosmetic composition of the present invention is a softening lotion, nutrient makeup, essence, lotion, nourishing cream, massage cream, pack, gel, ointment, patch, spray, linen agent, pastaase, cataplasma It may have a formulation such as, but is not limited to this.

In addition, the present invention provides a pharmaceutical composition for improving skin wrinkles containing at least two plant extracts selected from the group consisting of white pattern thistle extract, ginger extract, European equestrian extract, licorice extract and red shamrock extract. do.

In addition, the present invention provides a health food for improving skin wrinkles containing two or more plant extracts selected from the group consisting of white pattern thistle extract, ginger extract, European equestrian extract, licorice extract and red shamrock extract. .

The composition of the present invention significantly improves elasticity and wrinkles by significantly increasing the expression of decorin and biglycan, the major proteins that constitute the dermis of the skin, or by increasing the length of the chain of glycosaminoglycan bound to them. Since it has an improvement effect, it can be usefully used as a composition for improving skin wrinkles and elasticity.

1 is a Western blot showing the effect on the expression of decorin, biglycan and MMP-1 in skin fibroblasts when treated with white or white thistle extract, European equestrian extract, ginger extract and licorice extract alone or in combination. (Western Blot) Electrophoresis picture.
Figure 2a is a Western blot electrophoresis photograph showing the effect on the expression of decorin and MMP-1 in skin fibroblasts when treated with white or white thistle extract and red shamrock extract alone or in combination.
Figure 2b is a Western blot electrophoresis photograph showing the effect on the expression of decorin and MMP-1 in skin fibroblasts when treated with European equestrian extract and red shamrock extract alone or in combination.
Figure 3a is the treatment of the European equestrian extract and licorice extract alone, or in combination with the European equestrian extract, licorice extract, red shamrock extract or white pattern thistle extract, the effect on the expression of decorin in human skin application experiment Western blot electrophoresis photograph showing.
3B is a Western blot electrophoresis showing the effect on the expression of decorin in human skin application experiments, when white pattern thistle extract and red shamrock extract are treated alone or in combination, or when the licorice extract is treated together with the extract. It is a picture.

The present invention provides a skin cosmetic composition for improving skin wrinkles containing two or more plant extracts selected from the group consisting of white pattern thistle extract, ginger extract, European equestrian extract, licorice extract and red shamrock extract.

The term "extract" referred to in the present invention refers to a material extracted by an arbitrary method from plant raw materials, and thus extracts extracted from it, concentrates obtained therefrom, dry matter of the concentrates, powders, or fermentation products of the extracts, etc. It is used in the sense of including all. The extract can be obtained by extracting from a plant raw material or a dried product thereof, and the raw material of the extract can be used without limitation such as cultivated or commercially available.

The white patterned thistle extract contained in the composition of the present invention is known as a main component of a flavonoid complex called silymarin, and is well known for its hepatoprotective, antioxidant, and elastase inhibitory effects. According to one embodiment of the present invention, the white pattern thistle extract has an effect of increasing the expression level of proteoglycans such as decorin and biglycan and the chain length of glycosaminoglycan in a fibroblast culture experiment, thereby improving skin wrinkles. In addition, it is possible to have an effect on improving skin aging.

The ginger extract contained in the composition of the present invention contains antioxidant components such as shogaol and gingerol. According to one embodiment of the present invention, the ginger extract was found to have an effect of increasing the length of the chains of glycosaminoglycans of decorin and biglycan in a fibroblast culture experiment.

The European equestrian extract contained in the composition of the present invention contains a vegetable female hormone component and is widely used as a therapeutic agent for menopausal symptoms. According to one embodiment of the present invention, the European equestrian extract was found to have an effect of increasing the length of the chain of decorin, glycosaminoglycan of biglycan in a fibroblast culture experiment.

It is known that the licorice extract contained in the composition of the present invention has an anti-aging effect such as suppressing the expression of MMP-1 by ultraviolet rays through restoring antioxidant components and restoring the suppression of collagen expression. According to one embodiment of the present invention, the licorice extract was found to have an effect of increasing the expression of decorin and biglycan in fibroblast culture experiments.

The red shamrock extract contained in the composition of the present invention is widely used as a symptomatic agent for menopausal symptoms by containing a vegetable female hormone component similar to the European equestrian extract, and reduces epidermal thickness, skin, and ovaries in the skin of rats with ovaries removed. It is known that it can restore phenomena such as lack of blood vessels. According to one embodiment of the present invention, red shamrock extract was found to have an effect of increasing the expression level of decorin and biglycan in a fibroblast culture experiment.

When the plant extract is obtained by extracting from a plant raw material, conventional extraction methods known in the prior art, such as a solvent extraction method, an ultrasonic extraction method, a filtration method, and a reflux extraction method, can be used without limitation, and preferably a solvent extraction method or reflux. It can be prepared by using an extraction method, but is not limited thereto. The extraction process may be repeated several times, after which it may be further subjected to steps such as concentration or freeze-drying. Specifically, the obtained plant extract is concentrated under reduced pressure to obtain a concentrate, and the concentrate is lyophilized, and then a high concentration plant extract powder may be prepared using a grinder.

The plant extract may be extracted using water, an organic solvent or a mixture thereof as an extraction solvent. The organic solvent is an alcohol, preferably a C ₁ -C ₄ alcohol, such as methanol, ethanol, propanol or butanol, hexane (n-hexane), ether, glycerol, propylene glycol, butylene glycol, ethyl acetate, methyl acetate, Dichloromethane, chloroform, benzene, and mixed solvents thereof may be used, but are not limited thereto.

When two or more types of plant extracts or compounds derived therefrom are included in the cosmetic composition of the present invention, the extracts obtained by individually extracting the plants may be prepared and used in combination, or a mixture of the plants may be extracted and used. It is not limited.

The extraction may be performed at a temperature of 60 °C to 100 °C, preferably 65 °C to 95 °C, more preferably 70 °C to 90 °C, but is not limited thereto. When it is carried out outside the above range, the activity of increasing the expression of decorin and biglycan of the corresponding extract may be lowered.

The extraction may be performed for 1 hour to 20 hours, preferably 2 hours to 16 hours, and more preferably 3 hours to 12 hours, but is not limited thereto. When it is carried out outside the above range, the activity of increasing the expression of decorin and biglycan of the corresponding extract may be lowered.

The extraction may be performed 1 to 8 times, preferably 1 to 6 times, and more preferably 1 to 5 times, but is not limited thereto. The extract may be a single extract obtained from each extract or a mixed extract of extracts obtained from each extract, preferably a mixed extract of extracts obtained from each extract.

The plant extract may further perform a conventional fractionation process in order to increase the effect of increasing activity of decorin and biglycan expression. For example, the fraction obtained by passing the plant extract according to the present invention through an ultrafiltration membrane having a constant molecular weight cut-off value, and separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity) Active fractions obtained through various purification methods additionally carried out by the like are also included within the scope of the plant extract of the present invention. The chromatography may be column chromatography, for example, column chromatography using a filler selected from the group consisting of silica gel, Sephadex, LH-20, ODS gel, RP-18, polyamide, Toyopearl and XAD resin. It is possible to separate and purify the active fraction by performing, column chromatography may be carried out several times by selecting an appropriate filler as needed, but is not limited thereto. In using the chromatography, the elution solvent, elution rate, and elution time may be applied to a solvent, rate, or time generally used by those skilled in the art.

The cosmetic composition of the present invention, in addition to the above-mentioned plant extract, within the range not impairing the effect desired by the present invention, preferably may further contain other components and the like that can give a synergistic effect to the plant extract. For example, the cosmetic composition of the present invention, in addition to the plant extract, may include, without limitation, conventional adjuvants or carriers such as antioxidants, stabilizers, solubilizers, vitamins, pigments, and fragrances. In addition, the cosmetic composition of the present invention may further contain one or more active ingredients exhibiting the same or similar function to the plant extract or a compound derived therefrom, a stabilizer, a hydrating agent or an emulsifying accelerator, a salt for controlling osmotic pressure And/or auxiliary agents such as buffering agents.

The content of the plant extract of the present invention may be 0.0001 to 50% by weight relative to the total weight of the cosmetic composition, preferably 0.01 to 40% by weight, more preferably 0.1 to 20% by weight, and more preferably It may be 0.2 to 10% by weight, but is not limited thereto. When the content of the plant extract is less than 0.0001% by weight, it is not possible to obtain a skin wrinkle improvement effect through sufficient decolin and biglycan expression increasing activity, and when it exceeds 50% by weight, there is a marked effect of synergistic effect with increasing content There is a falling issue.

According to one embodiment of the present invention, the components included in the cosmetic composition of the present invention are commonly used in cosmetic compositions within a range that does not impair the effect desired by the present invention in addition to the above-mentioned two or more types of plant extracts as active ingredients. It may further include the components to be, for example, antioxidants, stabilizers, solubilizers, vitamins, pigments and customary adjuvants such as fragrances, and carriers.

The cosmetic composition of the present invention is not particularly limited in its formulation, and may be prepared in any formulation conventionally prepared in the art, for example, softening, longevity, essence, lotion, nourishing cream, massage Creams, packs, gels, ointments, patches, sprays, linen preparations, pastases, or cataplasmas can be prepared in a formulation. Here, when the formulation is an ointment, it is prepared to contain 50.0 to 97.0% by weight of petrolatum, and 0.1 to 5.0% by weight of polyoxyethylene oleyl-ether phosphate, in addition to the plant extract, and the softening and longevity includes polypropylene glycol, glycerin, etc. It is prepared to contain 1.0 to 10.0% by weight of alcohols, and 0.05 to 2.0% by weight of surfactants such as polyethylene oleyl ether and polyoxyethylene cured castor oil. Nutrient make-up and nutrient cream contain 5.0 to 20.0% by weight of oils such as squalane, petrolatum, octyldodecanol, and 3.0 to 15.0% by weight of wax components such as cetanol, stearyl alcohol, and wax, and essences are glycerin and propylene. It contains 5.0 to 30.0% by weight of polyhydric alcohols such as glycol. Massage cream is prepared by containing 30.0 to 70.0% by weight of oils such as liquid paraffin, petrolatum, and isononyl isononanoate in addition to the active ingredient, and the pack is peeled off containing 5.0 to 20.0% by weight of polyvinyl alcohol. In a pack or general emulsifying cosmetic, pigments such as kaolin, talc, zinc oxide, and titanium dioxide may be prepared as a wash off pack containing 5.0 to 30.0% by weight.

In the present invention, the conventional strategy for improving skin aging through the regulation of MMP-1 and collagen is deviated, which induces both a quantitative increase of the proteoglycans, decorin and biglycan, and an increase in the length of the glycosaminoglycan chain as a new target. A combination of possible plant extracts was discovered. The combination of plant extracts included in the composition of the present invention may restore the reduction of chain length and the reduction of decorin and biglycan occurring in aged skin. Since the decorin and biglycan are known to play a structural role and a protective role by interacting with collagen fibers and elastic fibers, it may have an indirect effect on reduction of collagen fibers and elastic fibers. In addition, in the case of the plant extracts used in the present invention, when treated with fibroblasts alone or in combination, a number of cases in which the expression of MMP-1 is reduced are also included.

When the cosmetic method of applying the cosmetic composition containing the plant extract of the present invention to human skin is performed, it protects the skin substrate damaged by ultraviolet rays, and specifically, improves skin wrinkles through increasing activity of decorin and biglycan expression Can get

In addition, the present invention provides a pharmaceutical composition for improving skin wrinkles containing at least two plant extracts selected from the group consisting of white pattern thistle extract, ginger extract, European equestrian extract, licorice extract and red shamrock extract. do.

The content of two or more plant extracts selected from the group consisting of the white pattern thistle extract, ginger extract, European equestrian extract, licorice extract and red shamrock extract contained in the pharmaceutical composition is 0.0001 to about the total weight of the pharmaceutical composition It is preferably 20.0% by weight, more preferably 0.001 to 1.0% by weight, but is not limited thereto. If the content of the plant extract is less than 0.0001% by weight, the effect of improving skin wrinkles cannot be obtained, and if it exceeds 20% by weight, there is a problem that it is not economical to use more than necessary plant extracts.

The route of administration of the pharmaceutical composition according to the invention includes oral, intravenous, intramuscular, intraarterial, transdermal, subcutaneous, intraperitoneal, intranasal, intestinal, topical, sublingual or rectal, parenteral administration is preferred, More preferably, it is applied in a topical application method by application. The parenteral includes subcutaneous, intradermal, intravenous, intramuscular, intralesional injection or infusion techniques.

The pharmaceutical composition of the present invention may further contain one or more active ingredients exhibiting the same or similar function to the plant extract. Each of the above compositions may be formulated and used in the form of oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, external preparations, suppositories, and sterile injectable solutions, according to a conventional method.

Solid preparations for oral administration include powders, granules, tablets, capsules, soft capsules, pills, and the like. Liquid preparations for oral use include suspensions, intravenous solutions, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used diluents, various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, can be included. . Formulations for parenteral administration include external preparations and sterile injection preparations such as powders, granules, tablets, capsules, sterilized aqueous solutions, liquids, non-aqueous solvents, suspensions, emulsions, syrups, suppositories, aerosols, etc. It can be used in the form of a formulation, preferably a cream, gel, patch, spray, ointment, warning agent, lotion, linen agent, pasta agent or cataplasma pharmaceutical composition for external use for the skin can be prepared and used. , But is not limited to this. Non-aqueous solvents and suspensions may include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. As a base for suppositories, witepsol, macrogol, tween 61, cacao butter, laurin butter, and glycerogelatin may be used.

The composition may further contain preservatives, stabilizers, hydrating or emulsifying accelerators, adjuvants such as salts and/or buffers for osmotic pressure control, and other therapeutically useful substances.

The pharmaceutical composition of the present invention can be variously changed according to various factors including the age, weight, general health, sex, administration time, administration route, discharge rate, drug combination, and severity of a specific disease.

Suitable dosages of the pharmaceutical compositions of the invention are variously prescribed by factors such as formulation method, mode of administration, patient's age, weight, sex, morbidity, food, time of administration, route of administration, rate of excretion, and response sensitivity. Can be. The dosage of the pharmaceutical composition of the present invention is in the range of 0.001 to 100 mg/kg on an adult basis. In addition, in the case of an external preparation, it is recommended to apply 1 to 5 times a day in an amount of 1.0 to 3.0 ml on an adult basis to continue for 1 month or more, but the above dosage does not limit the scope of the present invention.

The pharmaceutical composition of the present invention may be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy and biological response modifiers.

The plant extract of the present invention can significantly increase the expression of decorin and biglycan, which are major proteins constituting the dermis in human fibroblasts, and is a safe substance derived from natural products. It can be usefully used as an ingredient.

In addition, the present invention provides a health food for improving skin wrinkles containing two or more plant extracts selected from the group consisting of white pattern thistle extract, ginger extract, European equestrian extract, licorice extract and red shamrock extract. .

The content of the plant extract may be appropriately determined according to the purpose of use. The content of two or more plant extracts selected from the group consisting of white pattern thistle extract, ginger extract, European equestrian extract, licorice extract and red shamrock extract contained in the health food is 0.0001 to 20.0 wt% based on the total weight of the health food It is preferable, and it is more preferably 0.001 to 1.0% by weight, but is not limited thereto. If the content of the plant extract is less than 0.0001% by weight, the effect of improving skin wrinkles cannot be obtained, and if it exceeds 20% by weight, there is a problem in that it is not economical to use an amount more than necessary and the stability of the composition is lowered. However, in the case of long-term intake for health and hygiene purposes or for health control, the amount may be below the above range, and since there is no problem in terms of safety, the plant extract may be used in an amount above the above range. .

When the composition of the present invention is prepared as a health food, it may contain ingredients that are commonly added in food preparation, and include, for example, proteins, carbohydrates, fats, nutrients, flavoring agents and flavoring agents. Examples of the carbohydrate include monosaccharides such as glucose and fructose; Disaccharides such as maltose, sucrose, oligosaccharides, etc.; And polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the flavoring agent, natural flavoring agents such as taumatin, stevia extract and synthetic flavoring agent may be used. For example, when the health food of the present invention is prepared as a drink agent, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, juice, natural extract, etc. may be additionally included in addition to the plant extract.

There are no particular restrictions on the type of health food. Examples of foods to which the plant extract of the present invention can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, various soups, beverages, Teas, drinks, alcoholic beverages, and vitamin complexes are included, and they include all healthy foods in the ordinary sense.

Hereinafter, the present invention will be described in detail by examples and experimental examples.

However, the following examples and experimental examples are only for illustrating the present invention, and the contents of the present invention are not limited by the following examples and experimental examples.

Example 1. Preparation of plant extracts

Extracts of white pattern thistle, ginger, European equestrian, licorice and red shamrock flowers, respectively, included as active ingredients of the cosmetic composition of the present invention were prepared. To this end, 6 times distilled water was added to each of the plant raw materials, extraction was performed at 85°C for 12 hours, and the extract was filtered and cooled to 10°C. For the residue, distilled water was added 6 times, and extraction was performed three times for 12 hours, and the extract was filtered and then cooled to 10°C. Each extract was prepared by centrifuging the cooled extract at 4°C for 15 minutes at 6,000 rpm to remove the precipitate.

Experimental Example 1. Improvement effect of decorin, biglycan, MMP-1 according to the combination of plant extracts

Skin fibroblasts were cultured in DMEM (Dulbecco's Modified Eagle's Medium) medium containing 10% fetal bovine serum (FBS) to fill the cells, and then treated with 0% FBS-DMEM for 24 hours. While replacing the medium with fresh 0% FBS-DMEM, various plant extracts at a concentration of 0.2 mg/ml were treated on skin fibroblasts alone or in combination, and proteins were extracted from the cells after 24 hours. As the vehicle, 50% butylene glycol was used. The extracted protein sample was confirmed by Western blot method using each antibody to confirm the expression change of MMP-1, biglycan, decorin, and alpha-tubulin (α-Tubulin) was used as a control protein.

To perform Western blot, boil for 5 minutes by adding 4× lamelli buffer to the volume of 1/3 of the extracted protein sample, and then load 15 µl onto 10% SDS-PAGE gel for 2 hours at 20 mA/gel. During electrophoresis, it was transferred to a nitrocellulose membrane at 35 V for 2 hours in a refrigerator. The transfer-completed membrane was blocked with TBS-T buffer containing 3% skim milk powder (Tris-based saline buffer containing 0.05% Tween 20) for 1 hour at room temperature, washed with TBS-T buffer, and then washed with MMP-1, bi Glycan, decorin and alphatubulin antibodies were diluted 1:1,000 each, and reacted overnight in the refrigerator. After washing it again with TBS-T buffer, the HRP enzyme-bound secondary antibody was diluted 1:10,000 to react for 1 hour at room temperature, washed again with TBS-T buffer, and then transferred to the film through a chemiluminescent solution. The photosensitization confirmed the amounts of MMP-1, biglycan, decorin and alphatubulin proteins.

As a result, when the white pattern thistle extract, the European equestrian extract, the ginger extract and the licorice extract were treated alone, the expression of MMP-1 did not change or decreased, and the expression and chain length of biglycan and decorin were largely reduced. Band position). In addition, when the two types of plant extracts were treated by mixing two types, the expression of MMP-1 was largely reduced, and the overall expression and chain length of biglycans and decorin were further promoted (FIG. 1). .

In addition, when the white pattern thistle extract and red shamrock extract were treated alone, the expression of MMP-1 did not change or decreased, and the decorin increased the chain length or expression level, respectively. When the white pattern thistle extract and red shamrock extract were treated in combination, the expression reduction effect of MMP-1 was weakened, but the expression amount and chain length of decorin increased together (FIG. 2A).

In addition, when the European equestrian extract and the red shamrock extract were treated alone, the expression of MMP-1 decreased, and the chain length or expression level of decorin increased, respectively. When the European equestrian extract and red shamrock extract were treated in combination, the expression of MMP-1 was significantly decreased, and the amount of expression and chain length of decorin increased together (FIG. 2B).

Experimental Example 2. Improvement effect of decorin according to the combination of plant extracts in human skin application experiment

The plant extracts prepared in Example 1 at a concentration of 2% were applied to the buttocks skin of a woman over 65 years old alone or in combination for 3 times in 12 days (1st, 5th, 9th), and biopsy the tissues on the 12th for protein. Extracted. The solvent of the plant extract stock solution was 50% butylene glycol, and 4% was included in the applied composition, and the remaining 96% was 40% butylene glycol and 60% ethanol. Up to 6 sites per person were included in the test, and the extracted protein sample was confirmed by the expression change of decorin using each antibody by the Western blot method as described in Experimental Example 1, and beta-actin (β-actin) and The result of Ponceau S staining was used as a control protein.

As a result, when the European equestrian extract, licorice extract, white pattern thistle extract and red shamrock extract were treated alone, decorin generally increased the expression level and chain length. When the above four types of plant extracts were mixed and treated, decorin increased the expression level and chain length. In particular, the combination of the European equestrian extract and the licorice extract, the combination of the European equestrian extract and the white thistle extract, and the red rabbit In the combination of the grass flower extract and licorice extract, the expression level of decorin and the length of biglycan increased more significantly (FIGS. 3A and 3B ).

Preparation Example 1. Preparation of cosmetic composition

Flexible lotion, nutrient cream and lotion containing the combination of the plant extract of the present invention as an active ingredient were prepared as shown in Tables 1 to 3, respectively.

Preparation of flexible lotion Raw material Content (parts by weight) Licorice extract and white thistle extract 10.00 1,3-butylene glycol 1.00 Disodium LED 0.05 Allantoin 0.10 Dipotassium glyceride 0.05 Citric Acid 0.01 Sodium citrate 0.02 Glyceres-26 1.00 Arbutin 2.00 Hydrogenated castor oil 1.00 ethanol 30.00 Preservative a very small amount coloring agent a very small amount Flavor a very small amount Purified water Balance

Preparation of nutrition cream Raw material Content (parts by weight) Red shamrock extract and white pattern thistle extract 10.0 1,3-butylene glycol 7.0 glycerin 1.0 D-panthenol 0.1 Plant extracts 3.2 Magnesium aluminum silicate 0.3 PEG-40 stearate 1.2 Stearic Acid 2.0 Polysorbate 60 1.5 Lipophilic glyceryl stearate 2.0 Sorbitan sesquioleate 1.5 Cetearyl alcohol 3.0 Mineral oil 4.0 Squalane 3.8 Carlylic/Capric Triglyceride 2.8 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glyceride a very small amount Allantoin a very small amount Sodium hyaluronate a very small amount Tocopheryl Acetate Proper Triethanolamine Proper Preservative Proper Flavor Proper Purified water Balance

Preparation of lotion Raw material Content (parts by weight) Cetostearyl alcohol 1.6 Stearic acid 1.4 Lipophilic monostearate glycerin 1.8 PAGE-100 Stearate 2.6 Sesquiolein sorbitan 0.6 Squalene 4.8 Macadamia oil 2 Jojoba oil 2 Tocopherol acetate 0.4 Methyl polysiloxane 0.2 Echile Paraben 0.1 Tocopherol acetate 0.4 Methyl polysiloxane 0.2 Echile Paraben 0.1 Propyl paraben 0.1 1,3-butylene glycol 4 Methyl paraben 0.1 Shotgun 0.1 glycerin 4 d-pandenol 0.15 Allantoin 0.1 Red shamrock extract and European equestrian extract 3.5 Carbomer (2% aq. Sol) 4 Triethanolamine 0.15 ethanol 3 pt 41891 0.1 p-H20 48.3

Preparation Example 2. Preparation of pharmaceutical composition

<2-1> Preparation of ointment for external use on skin

An ointment for external use on the skin containing the combination of the plant extract of the present invention as an active ingredient was prepared as shown in Table 4 below.

Raw material Content (parts by weight) Purified water 100 glycerin 8.0 Butylene glycol 4.0 Floating paraffin 15.0 Beta glucan 7.0 Carbomer 0.1 European equestrian extract and licorice extract 20 Caprylic capric triglyceride 3.0 Squalane 1.0 Cetearyl Glucooid 1.5 Sorbitan stearate 0.4 Cetearyl alcohol 1.0 antiseptic Proper incense Proper Coloring Proper Wax 4.0

<2-2> Preparation of patch for external application to skin

A patch for external application of skin containing the combination of the plant extract of the present invention as an active ingredient was prepared as shown in Table 5 below.

Raw material Content (parts by weight) European equestrian extract and white patterned thistle extract 10.0 Hexylene glycol 20.0 Diethylamine 0.7 Polyacrylic acid 1.0 Sodium sulfite 0.1 Polyoxyethylene lauryl ether (E.0=9) 1.0 Polyhydroxyethylene cetyl stearyl ether 1.0 Viscous paraffin oil 2.5 Caprylic acid ester/capric acid ester (cetiol LC) 2.5 Polyethylene glycol 400 3.0 Deionized water 70

<2-3> Preparation of powder

Red shamrock extract and licorice extract············ 2 g

Lactose··························· 1 g

A powder was prepared by mixing the above components and filling the gas-tight fabric.

Claims (7)

As a cosmetic composition for improving skin wrinkles containing two or more types of plant extracts selected from the group consisting of white pattern thistle extract, European equestrian extract, licorice extract and red shamrock extract,
The plant extract is a combination of European equestrian extract and licorice extract; A combination of European equestrian extract and white pattern thistle extract; And a combination of red shamrock extract and licorice extract; cosmetic composition for improving skin wrinkles, characterized in that selected from the group consisting of. The method according to claim 1,
The extraction solvent used in the preparation of the plant extract is a cosmetic composition selected from the group consisting of water, organic solvents and mixtures thereof. The method according to claim 2,
The organic solvent is a cosmetic composition selected from the group consisting of alcohol, hexane, ether, glycerol, propylene glycol, butylene glycol, ethyl acetate, methyl acetate, dichloromethane, chloroform, benzene, and mixed solvents thereof. The method according to claim 3,
The alcohol is a cosmetic composition selected from the group consisting of methanol, ethanol, propanol and butanol. The method according to claim 1,
Cosmetic composition having a formulation selected from the group consisting of softening lotion, nutrient makeup, essence, lotion, nourishing cream, massage cream, pack, gel, ointment, patch, spray, linen agent, pastaase, and cataplasma. As a pharmaceutical composition for improving skin wrinkles containing two or more plant extracts selected from the group consisting of white pattern thistle extract, European equestrian extract, licorice extract and red shamrock extract,
The plant extract is a combination of European equestrian extract and licorice extract; A combination of European equestrian extract and white pattern thistle extract; And Combination of red shamrock extract and licorice extract; Pharmaceutical composition for improving skin wrinkles, characterized in that selected from the group consisting of. As a health food for improving skin wrinkles containing two or more kinds of plant extracts selected from the group consisting of white pattern thistle extract, European equestrian extract, licorice extract and red shamrock extract,
The plant extract is a combination of European equestrian extract and licorice extract; A combination of European equestrian extract and white pattern thistle extract; And combination of red shamrock extract and licorice extract; health food for improving skin wrinkles, characterized in that selected from the group consisting of.

Images