KR20200020405A - Composition for improving skin wrinkle comprising plant extract - Google Patents

Abstract

The present invention relates to a composition for reducing skin wrinkles. More particularly, the present invention relates to a composition for reducing skin wrinkles containing, as an active ingredient, two or more plant extracts selected from the group consisting of a Silybum marianum extract, ginger extract, Cimicifuga racemosa extract, licorice extract and Trifolium pratense flower extract. The composition of the present invention significantly increases the expression of decorin and biglycan, which are major proteins constituting the dermis of the skin, and the length of a glycosaminoglycan chain bound thereto, and thus is excellent in improving elasticity and reducing wrinkles, thereby effectively usable as a composition for reducing skin wrinkles and improving elasticity.

Description

COMPOSITION FOR IMPROVING SKIN WRINKLE COMPRISING PLANT EXTRACT}

The present invention relates to a composition for improving skin wrinkles, and more particularly, to the expression of glycosaminoglycans that increase or bind the expression of decorin and biglycan, which are substrate proteins of the dermis. It relates to a composition for improving skin wrinkles containing a plant extract as an active ingredient that can improve skin wrinkles and improve elasticity by increasing the length of the chain.

The skin is the largest body tissue that is the primary contact with the external environment and stimuli. It acts as the first defense against external substances and stimuli, as well as for metabolism in the body that regulates body temperature and body moisture. Plays an important role. Also, since the skin is the first organ to be seen from the outside, it is also a very important organ for expressing external beauty. This external beauty tends to behave similarly to health in modern society.

As the number of older people increases, not only does beauty of appearance become the standard of judgment that symbolizes youth and health, but also for the younger people, this external beauty is revealed as confidence and the competitiveness that affects their work ability. Because it is located. For this reason, efforts are being actively made to suppress skin aging and wrinkles on the academic and industrial sides.

The major change in skin aging is a decrease in wrinkles and skin elasticity, which is known to be due to structural changes in the dermis. The dermis is the structure of the skin below the epidermis, the outermost part of the skin, and collagen and elastic fibers make up the largest portion as a component. These collagen and elastic fibers play an important role in maintaining skin elasticity. The dermis of histologically aged skin is reduced in the thickness of the collagen fibers, the arrangement is poor, and the complex and sophisticated bonding of the elastic fibers is broken. This phenomenon is due to the expression of collagen and elastic fiber degrading enzymes due to continuous UV exposure, and is the main cause for wrinkle formation of elderly skin (Patent Document 1).

The development of functional cosmetics to improve skin wrinkles has been focused on the inhibition of enzymes such as matrix metalloproteinase (MMP) -1, which promotes the breakdown of collagen, and the increase of the expression of collagen, a key substrate protein, but it has practically satisfactory effects. The development of visible functional cosmetics is still a long way off. Ingredients such as retinoic acid and retinol are known to have an effective effect, but retinoic acid has strong side effects, and retinol has relatively low side effects but also side effects, and stability is weak, so it is easy to be active. There are various problems such as weakening.

The MMP is a determinant factor in collagen breakdown of the dermal layer, and many studies have been conducted to improve skin wrinkles by inhibiting the expression or activity of MMP. Activated MMPs are regulated by inhibitors such as 2-macroglobulin or tissue inhibitors of metalloproteinases (TIMPs), and many cells, including keratinocytes and fibroblasts in the skin, secrete MMPs ( Non-Patent Document 1). MMPs play a very important role in the formation of skin wrinkles because even once UV irradiation increases MMP activity in the skin and significantly disrupts collagen in the skin. However, there is little prior research on improving skin wrinkles by increasing expression of dermal proteins other than collagen.

Republic of Korea Patent Publication No. 10-2017-0037570

 Fisher G.J. et al., Photochem. Photobiol., 69, 154, 1999

It is an object of the present invention to provide a composition for improving skin wrinkles, which contains a plant extract having an effect of improving skin elasticity and improving skin wrinkles through an excellent activity of decorin and biglycan expression.

In order to achieve the above object, the present invention for improving skin wrinkles containing two or more plant extracts selected from the group consisting of white thistle extract, ginger extract, European horse riding extract, licorice extract and red shamrock extract as an active ingredient It provides a cosmetic composition.

According to one embodiment of the invention, the extraction solvent used in the preparation of the plant extract may be water, an organic solvent or a mixture thereof, the organic solvent is alcohol, hexane, ether, glycerol, propylene glycol, butylene glycol , Ethyl acetate, methyl acetate, dichloromethane, chloroform, benzene, or a mixed solvent thereof, but is not limited thereto. According to a preferred embodiment of the present invention, the alcohol may be a lower alcohol, such as methanol, ethanol, propanol or butanol, but is not limited thereto.

According to one embodiment of the present invention, the cosmetic composition of the present invention is a softening longevity, nourishing longevity, essence, lotion, nourishing cream, massage cream, pack, gel, ointment, patch, spray, linen, pasta, cataplasma It may have a formulation such as, but is not limited thereto.

In addition, the present invention provides a pharmaceutical composition for improving skin wrinkles containing two or more plant extracts selected from the group consisting of white thistle extract, ginger extract, European horse riding extract, licorice extract and red shamrock extract as an active ingredient do.

In addition, the present invention provides a health food for skin wrinkles containing two or more plant extracts selected from the group consisting of white thistle extract, ginger extract, European horse riding extract, licorice extract and red shamrock extract as an active ingredient. .

The composition of the present invention provides excellent elasticity enhancement and wrinkles by significantly increasing the expression of decorin and byglycans, which are the major proteins constituting the dermis of the skin, or by increasing the length of the chain of glycosaminoglycans bound thereto. Since it has an improvement effect, it can be usefully used as a composition for improving skin wrinkles and elasticity.

Figure 1 is a Western blot showing the effect on the expression of decorin, baiglican and MMP-1 in skin fibroblasts when treated alone or in combination with white thistle extract, European horse riding extract, ginger extract and licorice extract (Western Blot) Picture of electrophoresis.
Figure 2a is a Western blot electrophoresis picture showing the effect on the expression of decorin and MMP-1 in skin fibroblasts when treated alone or in combination of white thistle extract and red shamrock extract.
Figure 2b is a Western blot electrophoresis picture showing the effect on the expression of decorin and MMP-1 in skin fibroblasts when treated alone or in combination with European horseback riding extract and Red Rabbit flower extract.
Figure 3a is the effect on the expression of decorin in human skin application experiments when the European horseback extract and licorice extracts alone or in combination with licorice extract, red shamrock extract or white thistle extract Western blot electrophoresis showing.
Figure 3b is a Western blot electrophoresis showing the effect on the expression of decorin in human skin application experiments when the white thistle extract and red shamrock extract alone or in combination, or treated with licorice extract to the extract It is a photograph.

The present invention provides a skin cosmetic composition for improving skin wrinkles containing two or more plant extracts selected from the group consisting of white thistle extract, ginger extract, European horse riding extract, licorice extract and red shamrock extract as an active ingredient.

As used herein, the term "extract" refers to a material extracted from a plant raw material by any method, and limits the extract thus extracted, the concentrate obtained therefrom, the dried product of the concentrate, powder, or the fermentation product of the extract. It is used to include all without. The extract can be obtained by extracting from a plant raw material or its dried material, the raw material of the extract can be used without limitation, such as cultivated or commercially available.

White patterned thistle extract included in the composition of the present invention is known as the main component of the flavonoid complex called silymarin (Silymarin), liver protection effect, antioxidant effect, elastase inhibitory effect is well known and is used as a herbal medicine. According to one embodiment of the present invention, white patterned thistle extract has an effect of increasing the expression of proteoglycans and chain lengths of glycosaminoglycans such as decorin and biglycans in fibroblast culture experiments, thereby improving skin wrinkles. In addition, it may be effective in improving skin aging.

Ginger extract included in the composition of the present invention contains an antioxidant component such as Shogaol, Gingerol. According to one embodiment of the present invention, the ginger extract has been shown to have an effect of increasing the length of the chains of glycosaminoglycans of decorin, biglycans in fibroblast culture experiments.

European horseriding extract included in the composition of the present invention is widely used as a therapeutic agent for menopausal symptoms by containing a female female hormone component. According to one embodiment of the present invention, the European horseriding extract has been shown to have an effect of increasing the length of the chains of glycosaminoglycans of decorin, baiglican in fibroblast culture experiments.

Licorice extract contained in the composition of the present invention is known to have an anti-aging effect such as suppressing the expression of MMP-1 by ultraviolet rays and restoring the expression inhibition of collagen through the antioxidant component contained. According to one embodiment of the present invention, licorice extract has been shown to have an effect of increasing the amount of decorin, biglycan expression in fibroblast culture experiments.

Red rabbit flower extract in the composition of the present invention contains a plant female hormone component similar to the European horse riding extract is widely used as a therapeutic agent for menopausal symptoms, epidermal thickness reduction, gland and It is known to be able to recover from the lack of blood vessels. According to one embodiment of the present invention, the red shamrock flower extract has been shown to have an effect of increasing the amount of decorin, Baiglican expression in fibroblast culture experiments.

When the plant extract is obtained by extracting it from plant raw materials, conventional extraction methods such as solvent extraction, ultrasonic extraction, filtration and reflux extraction can be used without limitation, and preferably solvent extraction or reflux. It may be prepared by using an extraction method, but is not limited thereto. The extraction process may be repeated several times, after which it may be further subjected to steps such as concentration or lyophilization. Specifically, the obtained plant extract may be concentrated under reduced pressure to obtain a concentrate, and the concentrate may be lyophilized to prepare a high concentration plant extract powder using a grinder.

The plant extract may be extracted using water, an organic solvent or a mixture thereof as an extractant. The organic solvent is an alcohol, preferably C ₁ -C ₄ alcohols such as methanol, ethanol, propanol or butanol, hexane (n-hexane), ether, glycerol, propylene glycol, butylene glycol, ethyl acetate, methyl acetate, Dichloromethane, chloroform, benzene and mixed solvents thereof may be used, but are not limited thereto.

When two or more kinds of plant extracts or compounds derived therefrom are included in the cosmetic composition of the present invention, the extracts may be prepared by separately extracting the plants and then used in combination, or a mixture of the plants may be used. It is not limited.

The extraction may be carried out at a temperature of 60 ℃ to 100 ℃, preferably may be carried out at 65 ℃ to 95 ℃, more preferably may be carried out at 70 ℃ to 90 ℃, but is not limited thereto. When carried out outside the range, the activity of increasing the decorin and biglycan expression of the extract may be lowered.

The extraction may be carried out for 1 to 20 hours, preferably for 2 to 16 hours, more preferably for 3 to 12 hours, but is not limited thereto. When carried out outside the range, the activity of increasing the decorin and biglycan expression of the extract may be lowered.

The extraction may be performed once to eight times, preferably may be carried out once to six times, more preferably may be carried out once to five times, but is not limited thereto. The extract may be a single extract obtained from each extract or a mixed extract of extracts obtained from each extract, preferably a mixed extract of extracts obtained from each extract.

The plant extract may further perform a conventional fractionation process in order to increase the effect of increasing the decorin and biglycan expression. For example, fractions obtained by passing a plant extract according to the present invention through an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). Active fractions obtained through various purification methods additionally carried out in the above are also included within the scope of the plant extracts of the present invention. The chromatography may be column chromatography, for example column chromatography using a filler selected from the group consisting of silica gel, Sephadex, LH-20, ODS gel, RP-18, polyamide, Toyopearl and XAD resin The active fraction can be separated and purified by performing the step, and column chromatography can be carried out several times by selecting an appropriate filler as necessary, but is not limited thereto. In using the chromatography, an elution solvent, an elution rate, and an elution time may be applied to a solvent, a rate, or a time generally used by those skilled in the art.

The cosmetic composition of the present invention may further contain, in addition to the above-mentioned plant extracts, other ingredients that can give a synergistic effect to the above-mentioned plant extracts within a range that does not impair the effects aimed at by the present invention. For example, the cosmetic composition of the present invention may include, without limitation, conventional adjuvants or carriers such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors in addition to the plant extract. In addition, the cosmetic composition of the present invention may contain one or more active ingredients exhibiting the same or similar functions in addition to the plant extract or a compound derived therefrom, and stabilizers, wetting agents or emulsifiers, salts for controlling osmotic pressure And / or adjuvants such as buffers.

The content of the plant extract of the present invention may be 0.0001 to 50% by weight, preferably 0.01 to 40% by weight, more preferably 0.1 to 20% by weight, and more preferably, based on the total weight of the cosmetic composition. Preferably it may be 0.2 to 10% by weight, but is not limited thereto. When the content of the plant extract is less than 0.0001% by weight, it is not possible to obtain skin wrinkle improvement through sufficient decorin and biglycan expression increasing activity. There is a problem falling.

According to one embodiment of the present invention, the components included in the cosmetic composition of the present invention are commonly used in cosmetic compositions within the range that does not impair the effects desired by the present invention, in addition to the two or more plant extracts described above as active ingredients. And may include additional ingredients such as antioxidants, stabilizers, solubilizers, conventional auxiliaries such as vitamins, pigments and flavors, and carriers.

The cosmetic composition of the present invention is not particularly limited in its formulation, and may be prepared in any formulation conventionally prepared in the art, for example, softening cream, nutrient cream, essence, lotion, nourishing cream, massage It may be prepared in the form of a cream, pack, gel, ointment, patch, spray, linen, pasta or cataplasma. Here, when the formulation is an ointment, it is prepared to contain 50.0 to 97.0% by weight of petrolatum, and 0.1 to 5.0% by weight of polyoxyethylene oleyl-ether phosphate in addition to the plant extract, and the flexible cosmetic is polyvalent such as propylene glycol, glycerin, etc. It is prepared to contain 1.0 to 10.0% by weight of alcohols and 0.05 to 2.0% by weight of surfactants such as polyethylene oleyl ether and polyoxyethylene hydrogenated castor oil. Nourishing cream and nourishing cream contains 5.0 to 20.0% by weight of oils such as squalane, petrolatum and octyldodecanol, and 3.0 to 15.0% by weight of wax components such as cetanol, stearyl alcohol and beeswax, and the essence is glycerin and propylene. 5.0-30.0 weight% of polyhydric alcohols, such as glycol, are contained. Massage cream is prepared by containing 30.0 to 70.0% by weight of oil, such as liquid paraffin, petrolatum, and isononyl isononanoate, in addition to the active ingredient, and the pack is peeled off containing 5.0 to 20.0% by weight of polyvinyl alcohol. The pack or general emulsion cosmetic may be prepared as a wash off pack containing 5.0 to 30.0% by weight of pigments such as kaolin, talc, zinc oxide and titanium dioxide.

In the present invention, to avoid the skin aging improvement strategy through the regulation of the conventional MMP-1 and collagen, as a new target to induce a quantitative increase of the proteoglycans decorin and biglycan and increase the length of the glycosaminoglycan chain A combination of plant extracts was excavated. Combination of the plant extracts included in the composition of the present invention can restore the decrease in chain length and the decrease in decorin and biglycans occurring in aged skin. The decorin and byglycans are known to play a structural role and a protective role by interacting with collagen fibers and elastic fibers, and thus may indirectly reduce collagen fibers and elastic fibers. In addition, in the case of the plant extracts used in the present invention when treated alone or in combination with fibroblasts, the expression of MMP-1 also includes a large number of cases are included, as well as synergistic effects through it.

When the cosmetic method containing the plant extract of the present invention is applied to a human skin, the cosmetic method protects the skin substrates damaged by ultraviolet rays, and in particular, improves skin wrinkles by increasing the expression of decorin and byglycans. Can be obtained.

In addition, the present invention provides a pharmaceutical composition for improving skin wrinkles containing two or more plant extracts selected from the group consisting of white thistle extract, ginger extract, European horse riding extract, licorice extract and red shamrock extract as an active ingredient do.

The content of two or more plant extracts selected from the group consisting of the white thistle extract, ginger extract, European horse riding extract, licorice extract and red shamrock extract contained in the pharmaceutical composition is 0.0001 to the total weight of the pharmaceutical composition. It is preferably 20.0% by weight, more preferably 0.001 to 1.0% by weight, but is not limited thereto. If the content of the plant extract is less than 0.0001% by weight can not obtain a skin wrinkle improvement effect, if it exceeds 20% by weight there is a problem that is not economical using more than the necessary plant extract.

Routes of administration of the pharmaceutical compositions according to the invention include oral, intravenous, intramuscular, intraarterial, transdermal, subcutaneous, intraperitoneal, intranasal, intestinal, topical, sublingual or rectal, parenteral administration is preferred, More preferably, it is applied in a topical application manner by application. The parenteral includes subcutaneous, intradermal, intravenous, intramuscular, intralesional injection or infusion techniques.

The pharmaceutical composition of the present invention may further contain one or more active ingredients exhibiting the same or similar functions in addition to the plant extract. The composition may be used in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, oral formulations, external preparations, suppositories, and sterile injectable solutions, respectively, according to conventional methods.

Solid preparations for oral administration include powders, granules, tablets, capsules, soft capsules, pills and the like. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents, water and liquid paraffin. . Preparations for parenteral administration include powders, granules, tablets, capsules, sterile aqueous solutions, solutions, non-aqueous solutions, suspensions, emulsions, syrups, suppositories, aerosols, etc. It may be used in the form of a formulation, and preferably, an external skin pharmaceutical composition of a cream, a gel, a patch, a spray, an ointment, a warning, a lotion, a linen, a pasta, or a cataplasm may be prepared. It is not limited to this. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

The composition may further contain preservatives, stabilizers, hydrating or emulsifying accelerators, auxiliaries such as salts and / or buffers for controlling osmotic pressure, and other therapeutically valuable substances.

The pharmaceutical compositions of the present invention may vary depending on several factors, including the age, body weight, general health, sex, time of administration, route of administration, rate of release, drug combination and severity of the particular disease of the individual.

Suitable dosages of the pharmaceutical compositions of the present invention may vary depending on factors such as the formulation method, mode of administration, age, weight, sex, morbidity, condition of the patient, food, time of administration, route of administration, rate of excretion and response to reaction. Can be. The dosage of the pharmaceutical composition of the present invention is in the range of 0.001 to 100 mg / kg on an adult basis. In addition, in the case of external preparations, it is preferable to apply once to five times a day in an amount of 1.0 to 3.0 ml on an adult basis and continue for more than one month, but the dosage does not limit the scope of the present invention.

The pharmaceutical composition of the present invention may be used alone or in combination with methods using surgery, radiation therapy, hormonal therapy, chemotherapy and biological response modifiers.

The plant extract of the present invention can significantly increase the expression of decorin and biglycans, which are the major proteins constituting the dermis in human fibroblasts, and is a safe substance derived from natural products, and thus is effective in improving pharmaceutical compositions for skin wrinkles. It can be usefully used as a component.

In addition, the present invention provides a health food for improving skin wrinkles containing two or more plant extracts selected from the group consisting of white thistle extract, ginger extract, European horse riding extract, licorice extract and red shamrock extract as an active ingredient. .

The content of the plant extract may be appropriately determined depending on the intended use. The content of two or more plant extracts selected from the group consisting of white patterned thistle extract, ginger extract, European horse riding extract, licorice extract and red shamrock extract contained in the health food is 0.0001 to 20.0 wt% based on the total weight of the health food. It is preferable to be, and it is more preferable that it is 0.001 to 1.0 weight%, but it is not limited to this. If the content of the plant extract is less than 0.0001% by weight can not obtain a skin wrinkle improvement effect, if it exceeds 20% by weight is not economical using more than necessary amount, there is a problem that the stability of the composition is lowered. However, in the case of long-term intake for health and hygiene or health control, the amount may be below the above range, and since there is no problem in terms of safety, the plant extract may be used in an amount above the above range. .

When the composition of the present invention is prepared as a health food, it may include ingredients that are commonly added during food preparation, and include, for example, proteins, carbohydrates, fats, nutrients, seasonings and flavoring agents. Examples of such carbohydrates are monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose, oligosaccharides and the like; And sugars such as conventional sugars such as polysaccharides such as dextrin, cyclodextrin and the like and xylitol, sorbitol, erythritol. As the flavoring agent, natural flavoring agent taumartin, stevia extract and synthetic flavoring agent may be used. For example, when the health food of the present invention is prepared with a drink, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, fruit juice, natural extracts, etc. may be further included in addition to the plant extract.

There is no particular limitation on the kind of the health food. Examples of the food to which the plant extract of the present invention may be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, ice cream, various soups, beverages, Tea, drink, alcoholic beverages and vitamin complexes and the like, and includes all of the health food in the conventional sense.

Hereinafter, the present invention will be described in detail by Examples and Experimental Examples.

However, the following Examples and Experimental Examples are only for illustrating the present invention, and the content of the present invention is not limited by the following Examples and Experimental Examples.

Example 1 Preparation of Plant Extract

Extracts of white thistle, ginger, European horseback riding, licorice, and red rabbit flower, which are included as an active ingredient of the cosmetic composition of the present invention, were prepared, respectively. To this end, 6 times distilled water was added to each of the plant raw materials, followed by extraction at 85 ° C. for 12 hours, and the extract was filtered and cooled to 10 ° C. For the residue, 6 times distilled water was added, extraction was further performed three times for 12 hours, and the extracts were combined after filtration and cooled to 10 ° C. The cooled extracts were centrifuged at 6,000 rpm for 15 minutes at 4 ° C. to remove precipitates, thereby preparing respective plant extracts.

Experimental Example 1. Improvement effect of decorin, Baiglican, MMP-1 by the combination of plant extracts

Dermal fibroblasts were incubated in DMEM (Dulbecco's Modified Eagle's Medium) medium containing 10% Fetal Bovine Serum (FBS) to fill cells, and then treated with 0% FBS-DMEM for 24 hours. While replacing the medium with fresh 0% FBS-DMEM, various plant extracts at 0.2 mg / ml concentrations were treated alone or in combination with skin fibroblasts, and proteins were extracted from the cells after 24 hours. As vehicle, 50% butylene glycol was used. The extracted protein samples were confirmed by Western blot method using the respective antibodies to change the expression of MMP-1, Baiglican, and decorin, and alpha-tubulin was used as a control protein.

To perform Western blot, add 4 × lamelli buffer to the volume of 1/3 of the extracted protein sample and boil for 5 minutes, load 15 μl into 10% SDS-PAGE gel and 20 mA / gel for 2 hours. Were electrophoresed and transferred to nitrocellulose membrane at 35 V for 2 hours in the refrigerator. Transfer completed membranes were blocked with TBS-T buffer containing 3% skim milk powder (Tris-based saline buffer containing 0.05% of Tween 20) for 1 hour at room temperature, and washed with TBS-T buffer before MMP-1, Glycan, decorin and alphatubulin antibodies were diluted 1: 1,000 each and reacted overnight in the cold room. After washing this again with TBS-T buffer, the secondary antibody conjugated with HRP enzyme was diluted 1: 10,000 and reacted at room temperature for 1 hour. After washing with TBS-T buffer, the film was washed with chemiluminescent solution. Photosensitized to determine the amount of MMP-1, Baiglican, decorin and alphatubulin proteins.

As a result, when the white thistle extract, European horseback riding extract, ginger extract and licorice extract alone were treated, the expression of MMP-1 was unchanged or decreased. Band position) increased. In addition, when treated by mixing two of the four plant extracts, the expression of MMP-1 was largely reduced significantly, and the total glycemic expression and the increase in the chain length was further promoted as a whole glycine and decorin (Fig. 1) .

In addition, when the white thistle extract and Red Rabbit extract alone were treated, the expression of MMP-1 was unchanged or decreased, and decorin increased the chain length or the amount of expression, respectively. When the combination of the white patterned thistle extract and the extract of the red shamrock extract, the effect of reducing the expression of MMP-1 was weakened, but the expression and chain length of the decorin was increased together (Fig. 2a).

In addition, the expression of MMP-1 decreased when the European horseback riding extract and Red Rabbit flower extract were treated alone, and the chain length or expression amount of decorin increased, respectively. When the combination of the European horseback riding extract and Red Rabbit flower extract was treated, the expression of MMP-1 was significantly decreased, and the amount of decorin and the chain length were increased together (FIG. 2B).

Experimental Example 2. Improvement effect of decorin according to the combination of plant extracts in human skin application experiment

Plant extracts prepared in Example 1 at 2% concentration alone or in combination were patched three times (day 1, 5, 9) for 12 days on the hip skin of women 65 years of age or older. Extracted. The solvent of the plant extract stock solution was 50% butylene glycol, 4% was included in the applied composition, and the remaining 96% was 40% butylene glycol and 60% ethanol. Up to six sites per person were included in the test, and the extracted protein samples were confirmed by the expression of decorin using the respective antibodies by Western blot method as described in Experimental Example 1, and the beta-actin (β-actin) and Ponceau S staining results were used as a control protein.

As a result, when the European horseback extract, licorice extract, white thistle extract, and red shamrock extract alone were treated, decorin generally increased expression and chain length. When two kinds of the above four plant extracts were treated and treated, decorin increased expression and chain length together, in particular, the combination of the European horse riding extract and licorice extract, the combination of the European horse riding extract and the white thistle extract, and the red rabbit. The combination of grass flower extract and licorice extract significantly increased the amount of decorin expression and the length of balycans (FIGS. 3A and 3B).

Preparation Example 1 Preparation of Cosmetic Composition

Flexible lotion, nourishing cream and lotion containing a combination of the plant extract of the present invention as an active ingredient were prepared as shown in Tables 1 to 3, respectively.

Preparation of Flexible Lotion Raw material Content (parts by weight) Licorice Extract and White Thistle Extract 10.00 1,3-butylene glycol 1.00 Disodium ID 0.05 Allantoin 0.10 Dipotassium glycylizate 0.05 Citrix Acid 0.01 Sodium citrate 0.02 Glyceres-26 1.00 Arbutin 2.00 Hydrogenated Castor Oil 1.00 ethanol 30.00 Preservative a very small amount coloring agent a very small amount Flavor a very small amount Purified water Remaining amount

Manufacture of nutritional cream Raw material Content (parts by weight) Red Rabbit Extract and White Thistle Extract 10.0 1,3-butylene glycol 7.0 glycerin 1.0 D-panthenol 0.1 Plant extracts 3.2 Magnesium Aluminum Silicate 0.3 PEG-40 Stearate 1.2 Stearic acid 2.0 Polysorbate 60 1.5 Lipophilic glyceryl stearate 2.0 Sorbitan sesquioleate 1.5 Cetearyl Alcohol 3.0 Mineral oil 4.0 Squalane 3.8 Carlylic / Capric Triglycerides 2.8 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycylizate a very small amount Allantoin a very small amount Sodium hyaluronate a very small amount Tocopheryl Acetate Quantity Triethanolamine Quantity Preservative Quantity Flavor Quantity Purified water Remaining amount

Lotion Raw material Content (parts by weight) Cetostearyl alcohol 1.6 Stearic acid 1.4 Lipophilic Monostearic Acid Glycerin 1.8 Fiji-100 Stearate 2.6 Sesquioleate Sorbate 0.6 Squalene 4.8 Macadamia oil 2 Jojoba oil 2 Tocopherol Acetate 0.4 Methyl Polysiloxane 0.2 Eichparaben 0.1 Tocopherol Acetate 0.4 Methyl Polysiloxane 0.2 Eichparaben 0.1 Propylparaben 0.1 1,3-butylene glycol 4 Methylparaben 0.1 Xanthan Gum 0.1 glycerin 4 d-pandenol 0.15 Allantoin 0.1 Red Rabbit Flower Extract and European Horseriding Extract 3.5 Carbomer (2% aq.Sol) 4 Triethanolamine 0.15 ethanol 3 pt 41891 0.1 p-H20 48.3

Preparation Example 2 Preparation of Pharmaceutical Composition

<2-1> Preparation of skin ointment

Skin external ointment containing the combination of the plant extract of the present invention as an active ingredient was prepared as shown in Table 4 below.

Raw material Content (parts by weight) Purified water 100 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 15.0 Beta Glucan 7.0 Carbomer 0.1 European Horse Riding Extract and Licorice Extract 20 Caprylic Capric Triglycerides 3.0 Squalane 1.0 Cetearyl Glucoside 1.5 Sorbitan stearate 0.4 Cetearyl Alcohol 1.0 antiseptic Quantity incense Quantity Pigment Quantity Beeswax 4.0

<2-2> Preparation of an external skin patch

An external skin patch containing the combination of the plant extracts of the present invention as an active ingredient was prepared as shown in Table 5 below.

Raw material Content (parts by weight) European Horseriding Extract and White Thistle Extract 10.0 Hexylene glycol 20.0 Diethylamine 0.7 Polyacrylic acid 1.0 Sodium sulfite 0.1 Polyoxyethylene lauryl ether (E.0 = 9) 1.0 Polyhydroxyethylene cetyl stearyl ether 1.0 Viscous Paraffin Oil 2.5 Caprylic Acid Ester / Capric Acid Ester (cetiol LC) 2.5 Polyethylene Glycol 400 3.0 Deionized water 70

<2-3> Preparation of powder

Rhododendron Extract and Licorice Extract ... ············ 2 g

Lactose ································· 1g

The above ingredients were mixed and filled in airtight cloth to prepare a powder.

Claims (7)

A cosmetic composition for improving skin wrinkles comprising two or more plant extracts selected from the group consisting of white thistle extract, ginger extract, European horse riding extract, licorice extract and red shamrock extract. The method according to claim 1,
Extraction solvent used in the preparation of the plant extract is selected from the group consisting of water, organic solvents and mixtures thereof. The method according to claim 2,
The organic solvent is selected from the group consisting of alcohol, hexane, ether, glycerol, propylene glycol, butylene glycol, ethyl acetate, methyl acetate, dichloromethane, chloroform, benzene and a mixed solvent thereof. The method according to claim 3,
The alcohol is a cosmetic composition selected from the group consisting of methanol, ethanol, propanol and butanol. The method according to claim 1,
A cosmetic composition having a formulation selected from the group consisting of softening cosmetics, nourishing cosmetics, essences, lotions, nourishing creams, massage creams, packs, gels, ointments, patches, sprays, lining agents, pastases and cataplasmas. A pharmaceutical composition for improving skin wrinkles comprising as an active ingredient two or more plant extracts selected from the group consisting of white patterned thistle extract, ginger extract, European horse riding extract, licorice extract and red shamrock extract. Health food for skin wrinkle improvement comprising two or more plant extracts selected from the group consisting of white patterned thistle extract, ginger extract, European horse riding extract, licorice extract and red shamrock extract.

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