KR102345335B1 - Anti-stress compositon containing lepidium apetalum extracts - Google Patents

Abstract

The purpose of the present invention is to provide a composition for anti-stress based on natural products which can exhibit an antioxidant effect and can exhibit a stress reduction effect, and depression prevention and amelioration effect while containing Lepidium apetalum as an active ingredient. The present invention provides a composition which can be taken or used for a long time while being able to exhibit effects such as antioxidant, antidepressant, and anti-stress without the problem of side effects based on natural products.

Description

An anti-stress composition containing Lepidium extract as an active ingredient to relieve and improve stress {ANTI-STRESS COMPOSITON CONTAINING LEPIDIUM APETALUM EXTRACTS}

The present invention relates to a composition for anti-stress comprising a horseradish extract as an active ingredient. More specifically, it is to provide a composition for antioxidant, antidepressant and antistress based on natural products, which can exhibit not only an antioxidant effect, but also a stress reducing effect and depression prevention and amelioration effect, including Hemp horseradish. In addition, the composition can be used as general food, functional food, functional flavoring, etc. with high palatability.

It is recognized that the cause of diseases such as aging, cancer, cerebrovascular disease, and cardiovascular disease is free radicals or reactive oxygen species (ROS). The reactive oxygen species may mean oxygen with increased reactivity of oxygen essential for maintaining living organisms due to stress such as biological stress caused by pathogens invading living organisms or various environmental stresses caused by deterioration of the global environment, and the reactivity is High oxygen can cause serious physiological disorders and the like. Examples include a superoxide radical, a hydroxyl radical, hydrogen peroxide, or singlet oxygen.

Specifically, the free radicals or reactive oxygen species destroy cells, cut the connective tissue of the dermal layer of the skin, or cause cross-linking, so that not only skin-related diseases such as wrinkle formation, atopic dermatitis, acne or skin cancer, but also cancer, myocardial infarction ( Cardiovascular diseases including myocardial infarction, stroke, atherosclerosis, etc., and chronic neurodegenerative diseases such as amyotrophic lateral sclerosis or Parkinsion's disease (PD). It has been reported to cause neurodegenerative disease. In addition, free radicals or reactive oxygen species can peroxidize lipids in the human body to produce harmful substances called peroxidized lipids, and the peroxidized lipids act on blood vessels to cause various adult diseases such as arteriosclerosis and blood clots. has been

As described above, as the average lifespan increases and the aging of society progresses, interest in aging-related diseases such as aging and degenerative diseases is increasing, and due to economic growth and changes in diet, interest in metabolic syndrome or adult diseases is increasing. is increasing As interest in these diseases increases and interest in free radicals or reactive oxygen species reported as the cause of the disease also increases, interest in substances having an antioxidant effect is increasing.

As an antioxidant system that removes these free radicals or reactive oxygen species in the living body, high molecular antioxidant enzymes and vitamins such as superoxide dismutase (SOD), peroxidase (POD) or catalase (CAT) Antioxidants such as C, vitamin E or glutathione are present.

However, due to various causes and aging, including pollution, ultraviolet rays, or the biological stress, the body's antioxidant system gradually fails to function normally. A substance that can be used, that is, an antioxidant, is required to be supplied to the body. Efforts to prevent or treat the disease or delay aging by consuming antioxidants or foods having antioxidant effects are increasing according to the demand for supply of antioxidants in the body.

Conventionally, as synthetic antioxidants with excellent antioxidant power, BHA (butylated hydroxyanisole), BHT (butylated hydroxytoluene), or TBHQ (tertiary butylhydropuinone), etc. have been reported.

However, since some of the substances absorbed in the body are toxic, the synthetic antioxidant may become a teratogenic factor and a carcinogen, and may cause liver hypertrophy or increase microsomal enzyme activity in the liver, Safety issues such as causing pathological harm to organ tissues such as lungs and kidneys have been raised.

Therefore, in recent years, many studies are being conducted to develop antioxidants that are safer for the human body and have excellent antioxidant power, and the demand for natural antioxidants derived from these studies, that is, antioxidants obtained from natural substances, is increasing. are doing

Meanwhile, the World Health Organization (WHO) predicts that depression will become the world's second most afflicted disease in 2020. In Korea, according to the Health Insurance Review and Assessment Service, the number of people visiting hospitals for depression increased from 459,774 in 2010 to 475,670 in 2011 and 528,361 in 2012, an increase of about 15% over two years. .

However, all antidepressants currently used in clinical practice are drugs that enhance the action of monoamine neurotransmitters, and substances that inhibit serotonin reuptake and norepinephrine reuptake in neurons (SSRI, SNRI, TCAs, etc.) , substances that inhibit the breakdown of serotonin or dopamine, such as monoamine oxidase inhibitors.

However, currently only about 10-25% of depressed patients in Korea are receiving antidepressant treatment, and about 40% of them are known to stop treatment due to poor compliance due to side effects. It is reported that the non-responder accounted for 33%. Therefore, there is an urgent need for efforts on a composition having an antidepressant effect derived from a natural product.

In addition, as a representative term related to mental health, stress can be defined as "a reaction or change that is harmful to an organism caused by an external stimulus". The causes of stress and the physiological changes in the human body are diverse. Hormones such as adrenal medulla (eg, catecholamines), adrenocorticotropin (eg, adrenocorticotrophin) and endogenous opioids (eg, endorphin, enkephalin) are involved in the body's response to various stresses.

In particular, the content of corticosterone in plasma significantly increases due to immobilization stress, and the activity of catecholamine biosynthetic enzymes in the adrenal glands increases. Usually, the stress stimulus is stimulated by the cerebrum, etc., and the function of the adrenal gland is enhanced by the adrenocorticotropic hormone in the hypothalamus, and the weight increases, and the lipid decrease occurs.

On the other hand, the adrenal gland secretes other steroid hormones in the blood for cholesterol, blood sugar, alkaline phosphatase (ALP), lactate dehydrogenase (LDH), aspartate aminotransferase (AST or GOT) and alanine trans (alanine aminotrasgerase, ALT or GPT), etc. are increased, and ascorbic acid in the adrenal glands is released into the blood to significantly decrease the content, which is used as an indicator of stress.

Benzodiazepam (manufacturer: Sigma) is a representative anti-anxiety agent, and its mechanism of action is known to increase the affinity of GABA receptors, a representative inhibitory neurotransmitter in the central nervous system, to open adjacent Cl- channels more frequently to increase the permeability of Cl- ions. . Although the effect of benzodiazepam appears immediately, it is addictive and addictive, and it must be used according to the treatment of a specialist, and symptoms recur or withdrawal symptoms appear. Benzodiazepam also has side effects such as depression, psychosis, manic behavior, heightened anxiety, hostility, and rage. Orthostatic hypotension, respiratory depression, headache, chronic sleep disturbance, and liver disease have been reported.

In addition, in the treatment of electrical stress, 'Mendek' and 'Ahn Shin-hwan' as a mental anxiety stabilizer are known as the most widely used drugs, but these drugs have side effects such as psychosis, repeated mania and depression. In particular, Shin-Hwan Ahn is an herbal medicine that has been used for a long time when anxiety, shortness of breath, chest jump, surprise, forgetfulness, cold sweat, sweat flow during sleep, natural discharge of semen, and chest pain.

As described above, when synthetic compounds are used in stress treatment, there is an urgent need for alternative natural products or antidepressants or antistress agents derived from natural products to reduce these side effects.

Looking at the prior literature, Korean Patent No. 0513489 discloses a lotus root extract for antidepressant treatment, and Korean Patent Publication No. 2013-0086459 discloses a pharmaceutical composition for preventing and treating fast-acting depression containing sanjoin extract as an active ingredient. This is being disclosed. On the other hand, in order to exhibit antioxidant, antidepressant and anti-stress effects based on natural products that can be taken or used for a long time without any side effects, research on more diverse natural materials is needed.

KR 10-0513489 B1 KR 10-2013-0086459 A

It is an object of the present invention to provide a composition for antioxidant, antidepressant and anti-stress based on natural products, which can exhibit not only an antioxidant effect, but also a stress reduction effect and a depression prevention and amelioration effect, including Hemp horseradish.

It is an object of the present invention to provide a composition that can be taken or used for a long time while being able to exhibit effects such as antioxidants, antidepressants, and antistresses without any side effects based on natural products.

It is an object of the present invention to provide a general food, health functional food, palatable fragrance, etc. comprising the composition.

In order to achieve the above object, the composition for anti-stress according to an embodiment of the present invention includes a horseradish extract.

The extract is extracted using an extraction solvent selected from the group consisting of water, C1 to C6 lower alcohols, and mixtures thereof.

The composition exhibits an antidepressant effect.

The composition is to further include a natural extract selected from the group consisting of red pepper leaf extract, sagebrush extract, chazugi extract, and mixtures thereof.

The perfume for improving stress according to another embodiment of the present invention may include the anti-stress composition.

The food composition for preventing depression according to another embodiment of the present invention may include the anti-stress composition.

The pharmaceutical composition for preventing and treating depression according to another embodiment of the present invention may include the anti-stress composition.

Hereinafter, the present invention will be described in more detail.

The composition for anti-stress according to an embodiment of the present invention includes a horseradish extract.

Throughout this specification, 'extract' has the meaning commonly used as a crude extract in the sugar leaf system as described above, but in a broad sense also includes a fraction obtained by additionally fractionating the extract. That is, the extract includes not only those obtained using the above-described extraction solvent, but also those obtained by additionally applying a purification process thereto. For example, a fraction obtained by passing the extract through an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (prepared for separation according to size, charge, hydrophobicity or affinity), etc. The fraction obtained through the purification method is also included in the extract of the present invention.

Throughout this specification, when a part "includes" a certain component, it means that other components may be further included, rather than excluding other components, unless otherwise stated.

Throughout this specification, the term "combination of these" included in the expression of the Markush form means one or more mixtures or combinations selected from the group consisting of the components described in the expression of the Markush form, and the components It is meant to include one or more selected from the group consisting of.

Throughout this specification, the term "prevention" refers to any action that suppresses or delays the onset of arthritis by administration of the composition, and "treatment" or "improvement" means that the symptoms are improved or beneficial by administration of the composition Any action that changes

The extract used in the present invention may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze-drying or spray-drying.

"Antioxidant" as used in the present invention refers to exhibiting the effect of reducing active oxygen and inhibiting oxidation or aging of cells.

Depression as used in the present invention, also called depressive disorder, refers to a disease that causes a decrease in daily functioning by causing various cognitive, mental, or physical symptoms with a decrease in motivation and depression as the main symptoms. On the other hand, "anti-depression" as used in the present invention refers to the effect of being able to prevent and improve gastric depression or depressive disorder, and to improve motivation and depression.

The definition of stress in the present invention is slightly different for each scholar, but stress refers to a detrimental response or change to an organism caused by an external stimulus. In general, when the stimulus on the living body exceeds a certain level, it acts invasively. At this time, the organism responds to a certain threat regardless of the type of stimulus, such as tension headache, migraine, high blood pressure, indigestion, fatigue, pain, It causes hair growth and roughness of the skin, and if it continues chronically, it results in a nonspecific general adaptive syndrome, such as various neurosis and gastric ulcer, which is defined as stress (Selye, 1958, Longmans Green). and Co.).

In particular, various diseases such as mental disorders such as depression, digestive disorders such as neurosis and duodenal ulcer, endocrine disorders such as stress hyperthyroidism, skin disorders such as stress spots or acne, and cardiovascular disorders such as arrhythmias or angina pectoris It has been reported that the disease is caused by stress.

"Anti-stress" as used in the present invention refers to the effect of preventing or ameliorating the aforementioned stress or diseases caused by stress.

The Lepidium apetalum is a biennial herb of the Brassica family, a naturalized plant, native to North America. It grows anywhere along the roadside or in a clearing, prefers dry soil, and is about 30 to 60 centimeters tall. Stems stand upright and branches are divided at the upper part and spread like a broom. The leaves from the root spread radially and are pinnate compound leaves. The small leaves are about 3-5 centimeters long and have long petioles. The leaves from the stem are alternate phyllotaxis, have no petiole, have serrations on the edge, and have an inverted lancet shape and become a line shape toward the end. White flowers bloom in May-July and hang on raceme at the end of branches and main stems. In June-July, the young fruit is about 3 mm long and 2 mm wide, round and flat, and the tip is slightly concave. The reddish-brown seeds have white wings on the edge.

When the composition for anti-stress of the present invention contains the extract of Lepidoptera, it can exhibit the effect of preventing and alleviating stress and preventing and ameliorating depression along with antioxidant activity.

In addition, through the composition for anti-stress, it is possible to prevent and improve various diseases that may be caused by stress.

The Lepidium extract is extracted using an extraction solvent selected from the group consisting of water, C1 to C6 lower alcohols, and mixtures thereof.

Preferably, the solvent may be any one selected from the group consisting of water, ethanol, and mixtures thereof. In the case of the solvent, the yield for the active ingredient exhibiting antioxidant, anti-stress and anti-depressant effects can be maintained at a high level, and thus a more excellent effect can be exhibited compared to a relatively small content.

The composition for anti-stress will further include a natural extract selected from the group consisting of red pepper leaf extract, cauliflower extract, chazugi extract, and mixtures thereof.

The red pepper leaf refers to a leaf of hot pepper.

The pan-tailed grass grows in a sunny valley in a mountain valley, and the height is 30 to 80 cm. The rhizome is short and thick, and there are many fine roots. The leaf attached to the root is alternate phyllotaxis, the petiole is long, broad egg-shaped, gradually narrows and has a sharp tip, and the bottom is in the shape of a heart. The edge of the leaf is flat and the back side is white. Leaves are 5 to 10 cm long and 3 to 7 cm wide. The leaves on the stem are similar to this, but the petiole is short and the leaves are small. The sheath is membranous (膜質: translucent like thin paper). Flowers bloom in June or July in light pink or white, and hang on the inflorescence. There are no petals, and sepals are divided into 5 pieces. There are 8 stamens, longer than the calyx, the anthers are light reddish purple, and there is a small gland [腺] at the bottom of the stamens. Fruits are aqueous and ripen in September or October, are wrapped in calyx, and have 3 ridges. The young leaves and stems are edible, and the rhizomes are used to relieve heat, control competition, and eliminate inflammation of boils. It is distributed in Korea, northeastern China, Heilong River, and Ussuri River.

The chazugi (Perilla frutescens var. acuta) has a straight stem, a height of 20 to 80 cm, a square cross section, a purple color, and a fragrance. The leaves are opposite, broad egg-shaped, pointed at the tip, rounded at the bottom, and serrated at the edge. There are hairs on both sides of the leaves, long hairs on the veins on the back side, and long petioles. Flowers bloom in August or September in light purple and run in raceme at the ends of stems and branches. The calyx has hairs and is split into two, and the upper part of the split pieces is split into three again, and the lower piece is split into two again. The corolla is short, cylindrical, and the tip is lip-shaped, and the lower lip is slightly longer than the upper lip. There are 4 stamens, and 2 of them are long. The fruit is a branch (separate: each fruit divided from the fissionaceae), is round, has a diameter of 1.5mm, and is contained in a calyx.

When the red pepper leaf extract, the banyan tree extract and the chazugi extract are further included in the anti-stress composition of the present invention, a synergistic effect on anti-stress can be exhibited.

Preferably, the anti-stress composition comprises a horseradish extract, 0.1 to 20 parts by weight of a red pepper leaf extract based on 100 parts by weight of the horseradish extract; It will contain 5 to 50 parts by weight of sagebrush and 0.1 to 20 parts by weight of chazugi extract.

According to the above range, antioxidant, antidepressant and anti-stress effects may be significantly increased due to the synergistic effect of the interaction of each extract. Therefore, it can produce a high effect with a smaller content, and there is no problem of cytotoxicity and other side effects even when taken for a long time.

More preferably, the anti-stress composition further comprises an extract of Lamiaceae, a red snow tea extract and an extract of Cheonmundong.

Preferably, the anti-stress composition comprises a Lepidium extract, 0.1 to 20 parts by weight of a red pepper leaf extract based on 100 parts by weight of the Lepidium extract; 5 to 50 parts by weight of sagebrush; 0.1 to 20 parts by weight of chazugi extract; 0.1 to 10 parts by weight of Lamiaceae extract; It will contain 0.1 to 10 parts by weight of red snow tea extract and 0.1 to 10 parts by weight of Cheonmundong extract.

The "Lamiaceae" is a perennial herb commonly grown in mountains and fields across the country, and is also distributed in China and Japan. There is a rhizome. Stems are reddish, hairy, and 20-60cm tall. The leaves are opposite, ovate or ovate oval, with a flat edge or some serrations. Flowers are densely hung on the inflorescence at the tip of the stem in May-July, and are purple, pink, white, and lip-shaped. The calyx is lip-shaped, divided into 5 parts. The lower lip of the corolla is divided into three parts. Stamens 4, 2 are long. The fruit is a soybean, split into 4, and ripens to yellowish brown.

The "Snow Tea" (Snow Tea, 紅雪茶) refers to a dried moss plant that grows wild in an old juniper tree in an alpine region.

The "Cheonmun-dong" grows on the seashore, and the rhizome is short and many cylindrical roots with pointed ends spread in all directions. The lower part of the stem has egg-shaped scales. Stems are green, reaching 1 to 2m in length, tendrils, and leaf-like branches run in groups of 1 to 3 and bend like a bow. Flowers bloom in May or June, hang 1 to 3 in leaf axils, light yellow, and small flower stalks have joints in the center and are similar to the length of petals. Inflorescences are yellowish-white in color and have a long oval shape. There are 6 petals and 6 stamens, and 3 styles. The fruit is a berry, round, about 6mm in diameter, matured in white, and contains 1 black seed.

When the Lamiaceae extract, red snow tea extract and Cheonmundong extract are further included in the above range, the interaction of each active ingredient not only exhibits an additional synergistic effect on antioxidant, antidepressant and antistress, but also significantly increases flavor and palatability. can pay Accordingly, it is possible to widely distribute food and fragrance compositions with excellent palatability.

A perfume for improving stress according to another embodiment of the present invention includes the anti-stress composition.

When exemplifying the embodiment of the invention for the perfume, the composition of the present invention may be implemented in the form of a perfume, a diffuser, a scented candle, a massage oil, a body oil, a fragrance lamp, an aroma stick, a spray, a soap (eg, beauty soap) and a bath agent. can

In one embodiment, the composition of the present invention may be embodied as a perfume, a bath agent or a perfume formulation, in which case the perfume composition of the present invention may further include components commonly added to perfume manufacturing in addition to the mixed essential oil. These ingredients include fragrances, preservatives, stabilizers, colorants, antibacterial agents, deodorants, and disinfectants.

A food composition for preventing depression according to another embodiment of the present invention includes the anti-stress composition.

At this time, the amount of the extract in the food or beverage may be added in an amount of about 0.01 to 15% by weight of the total food weight, and the health drink composition may be added in a ratio of about 0.01 to 10g based on 100 ml, but is not limited thereto.

When the food composition of the present invention is a beverage composition, there is no particular limitation on the other ingredients except for containing the extract as an essential ingredient in the indicated ratio, and it may contain various flavoring agents or natural carbohydrates as additional ingredients like conventional beverages. can Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; disaccharides such as maltose, sucrose and the like; and polysaccharides such as conventional sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (taumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) may be used, but are not limited thereto. The proportion of the natural carbohydrate may be generally in the range of about 1 to 20 g per 100 ml of the composition of the present invention, but may not be limited thereto.

In addition to the above, the composition of the present invention contains various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic flavoring agents and natural flavoring agents, coloring agents and thickeners (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its salts. salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonation agents used in carbonated beverages, etc., but may not be limited thereto.

In addition, the composition of the present invention may contain natural fruit juice and pulp for the production of fruit juice beverages and vegetable beverages, and these components may be used independently or in combination. The proportion of these additives is not critical, but may be selected from about 0.1 to about 20 parts by weight per 100 parts by weight of the composition of the present invention, but is not limited thereto.

"Functional food" as used in the present invention is a food manufactured and processed by extracting, concentrating, refining, mixing, etc., a specific ingredient as a raw material for the purpose of health supplementation, or including health supplement food. In addition, it is a food designed and processed to sufficiently exert the biological control functions of the food ingredients, such as biological defense, regulation of biological rhythm, prevention and recovery of diseases, and functions related to disease prevention and recovery from diseases. It is assumed to include all that it has.

The present invention provides a composition for anti-stress based on natural products, which can exhibit not only an antioxidant effect, but also a stress-reducing effect and depression prevention and improvement effect, while containing Lepidium extract as an active ingredient.

The present invention provides a composition that can be taken or used for a long period of time while being able to exhibit effects such as antioxidant, antidepressant and anti-stress without the problem of side effects based on natural products.

1 relates to the antioxidant effect of a composition according to an embodiment of the present invention.
2 is related to the antioxidant effect of the composition according to an embodiment of the present invention.
3 relates to an antidepressant effect of a composition according to an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in detail so that those of ordinary skill in the art can easily carry out the present invention. However, the present invention may be embodied in several different forms and is not limited to the embodiments described herein.

[Preparation Example: Preparation of plant extract]

1. Preparation of Lepidium extract

고온에서 증발시킨다. 그리고 남은 시료는 동결건조 시켜두었다. 본 실험에 사용한 다닥냉이 추출물(LME)은 다닥냉이의 원재료에서 수득률은 10.5%로 추출되었다.Put 3 g of lyophilized horseradish into a 15 ml tube, 1 mg, and put 10 ml of 70% ethanol again. Put the sample in a rounded mixed machine for 18 hours, mix and extract. After extraction, after centrifugation at 3000 rpm for 10 minutes, only the supernatant is collected. Ethanol contained in the supernatant collected in this way was 40

Evaporate at high temperature. And the remaining sample was freeze-dried. Lepidium extract (LME) used in this experiment was extracted from the raw material of horseradish with a yield of 10.5%.

2. Preparation of other plant extracts

Red pepper leaf extract (AE) was prepared by extracting dried and pulverized red pepper leaves with 70% ethanol (ethanol). In addition, the extract (BE), chrysanthemum extract (CE), Lamiaceae extract (DE), red snow tea extract (EE) and Cheonmundong extract (FE) were also prepared in the same manner as above.

3. Preparation of Complex Extracts

In order to confirm the synergistic effect of mixing each extract, each extract was mixed according to the composition shown in Table 1 below to prepare a complex extract.

MX1 MX2 MX3 MX4 MX5 MX6 MX7 MX8 MX9 LME 100 100 100 100 100 100 100 100 100 AE 0.01 0.1 10 20 30 10 10 10 10 BE One 5 30 50 100 30 30 30 30 CE 0.01 0.1 10 20 30 10 10 10 10 DE - - - - - 0.01 0.1 10 20 EE - - - - - 0.01 0.1 10 20 FE - - - - - 0.01 0.1 10 20

(Unit: parts by weight)

[Experimental example: antioxidant, antidepressant, anti-stress test]

1. Animal test methods and animal groups

(1) Animal testing method

, 습도는 55-60 %를 유지하여 사육하였다. 주간-야간 주기는 12시간으로 조절하였다. 일반배합사료(PMI Nutrition LLC. Manufactured in the USA. 5L79) 및 음수는 제한을 두지 않았고 일주일간 사육실 환경에 적응기를 가진 후 실험을 실시하였다. 실험은 모두 주간에 실시하였다.Four-week-old Spraque-Dawley rats with a body weight of 100 to 120 g (Korea Central Animal, Gyeonggi-do, Korea) were purchased from 30 females and the room temperature was 20±2.

, the humidity was maintained at 55-60% for breeding. The day-night cycle was adjusted to 12 hours. There were no restrictions on general formula feed (PMI Nutrition LLC. Manufactured in the USA. 5L79) and drinking water, and the experiment was conducted after a period of adaptation to the breeding room environment for a week. All experiments were conducted during the day.

(2) group of animals

5 animals per group were divided into 3 groups. Group 1 is a control group administered with water orally, group 2 is a group administered with a commercially available antidepressant (Imipramine: 0.178 mg/kg (rat weigh)), and group 3 is a group administered with horseradish 15 mg/150 g (rat weigh). was orally administered.

2. Antioxidant test

(1) Measurement of DPPH radical scavenging activity - Antioxidant possessed by horseradish

DPPH (2,2-diphenyl-1-picrylhydrazyl)

에서 30분 동안 incubation시켰다. 이후 spectrophotometer를 이용하여 517 nm에서 흡광도를 측정하였다.The hydrazyl of DPPH (Diphenylpicryl hydrazyl) used in this experiment has the property of easily accepting hydrogen atoms because the nitrogen atom is in an unstable state. 1.9 ml of 0.2 mM DPPH solution (99.5% ethanol) was added to 0.1 ml (control: 99.5% ethanol) of a solution prepared in 5 concentrations of 0.05, 0.1, 0.2, 0.5, and 1 mg/ml of dried horseradish for each material. After shaking for 10 seconds with a vortex mixer, 37

incubated for 30 min. Then, the absorbance was measured at 517 nm using a spectrophotometer.

As a positive control drug, L-ascorbic acid was prepared at 5 concentrations of 0.05, 0.1, 0.5, and 1 mg/ml (99.5% ethanol) and measured. The antioxidant activity of each sample was expressed as % Antioxidant activity (electron donating ability) against DPPH.

The results are shown in [Fig. 1] below.

(2) Xanthine oxidase inhibitory activity measurement

에서 5분간 반응시킨 후 1 N HCl 1 ml를 가하여 반응을 종료시킨 다음, 반응액 중에 생성된 uric acid를 292 nm에서 흡광도를 측정하였다. Xanthin oxidase저해 활성은 시료용액의 첨가군과 무첨가군의 흡광도 감소율로 나타내었다.To 0.1 ml of sample solution and 0.6 ml of 0.1 M potassium phosphate buffer of pH 7.5, 0.2 ml of substrate solution in which 2 mM xanthine was dissolved was added, and 0.2 unit/ml xanthine oxidase 0.1ml was added to 37

After reacting for 5 minutes, 1 ml of 1 N HCl was added to terminate the reaction, and the absorbance of uric acid generated in the reaction solution was measured at 292 nm. Xanthin oxidase inhibitory activity was expressed as a decrease in absorbance in the group with and without the sample solution.

Xanthine oxidase is an enzyme that acts as a rate-limiting enzyme in the terminal oxidation of all purines and as a source of oxidizing agents such as superoxide radicals and hydrogenperoxide. The inhibition of superoxide anion by the enzyme of xanthine/xanthine oxidase is indicated by the scavenging action of superoxide anion and inhibition of the xanthine oxidase enzyme, and has a biological significance by inhibiting the generation of free radicals. The superoxide radical scavenging effect of 50 μM of allopurinol as a positive control was compared.

The results are shown in [Fig. 2] below. On the other hand, referring to [Fig. 2] below, it can be seen that the clearing effect is about 18% higher at 1 mg/ml than the positive control, allopurinol 50 μM.

3. Antidepressant behavior test (Forced Swimming Test, FST)

After the animals arrived at the kennel, they were acclimatized to the kennel environment for 14 days and acclimatized by conducting a forced swimming test for 15 minutes in all 3 groups to acclimatise to the water. Imipramine) was administered orally. After missing 5 minutes of the forced swimming test, it appears that they are actively trying to escape at the beginning, but they soon fall into a sleep fear and maintain an immobility according to the degree of depression, so immobility time was measured. The experiment was repeated 3 times.

On the other hand, the results are shown in [Fig. 3] below.

4. Stress improvement effect test

Ten men and women who consented to this experiment were randomly divided into a control group (placebo) who took a placebo and an experimental group who took Lepidium extract (LME), each of which was arbitrarily divided into 5 people each, and consumed 400 mg each in the form of a tea drink for 4 weeks. In order to examine the psychological health improvement effect, PWI (Psychosocial Well-being Index), a social and psychological health measurement test, was conducted before and after intake. For objective comparison, the value of the control group was fixed at 10, the index was converted on a 10-point scale, and the index was evaluated as an index of 1 to 10, and the results are shown in [Table 2] below. In the following index, the lower the number, the better the stress improvement effect.

placebo LME 10 8.6

(Unit: Index)

Referring to [Table 2], it can be seen that the continuous use of the Lepidium extract according to the present invention can help relieve stress. In particular, it can be seen that self-confidence and mental and physical stability can be increased according to the PWI index. Therefore, when the horseradish extract is continuously used, stress can be alleviated, and the prevention and improvement effects of diseases caused by stress can be exhibited.

4. Antioxidant, antidepressant, anti-stress test for complex extracts

Antioxidant (AO), antidepressant (AP), and antistress (AS) effects were evaluated for the complex extracts MX1 to MX9 in the same manner as above. In addition, in order to check whether a synergistic effect compared to Lepidium extract (LME), LME was set as a fixed value, and the activities of MX1 to MX9 were compared.

Therefore, for objective comparison, the following evaluation indices are shown by fixing the LME result to 1 for AO and AP, and converting the values of MX1 to MX9 into indices of 1 to 10 compared to the LME. AO and AP in the above index, the higher the number, the better the effect. On the other hand, in AO and AP, the index calculated as 1 and less than 1 is also indicated as 1. Therefore, the index indicated by 1 in AO and AP does not have a synergistic effect, or its effect is inferior to that of using LME alone.

In addition, in the case of AS, the result of LME was fixed to 10, and the values of MX1 to MX9 were compared with LME and converted into an exponent of 1 to 10. In the above index, AS is the lower the number, the better the effect. On the other hand, the index calculated to exceed 10 by 1 in AS is also denoted as 10. Therefore, the index expressed as 10 in AS does not have a synergistic effect or has a lower effect than the use of LME alone.

The results are shown in [Table 3] below.

LME MX1 MX2 MX3 MX4 MX5 MX6 MX7 MX8 MX9 AO One One 3.3 4.1 4.2 1.4 3.4 4.8 5.1 2.9 AP One One 2.4 3.1 2.9 One 2.9 4.7 5.1 1.1 AS 10 10 8.9 8.5 8.8 9.9 8.7 6.3 6.6 8.2

(Unit: Index)

Referring to [Table 3], in the case of MX1, there was no synergistic effect on the complex extract, and in the case of MX5, there was no significant synergistic effect while relatively increasing other extracts other than LME, and it was confirmed that some indices were lower than LME. could

However, as in MX2 to MX4, according to the embodiment in which the red pepper leaf extract, the sagebrush extract and the chazugi extract are included in a specific content range, when using the Lepidium extract alone as a mutual combination of the active ingredients included in each extract It can be seen that there is a synergistic effect. In addition, in the case of MX3, it was found that antioxidant activity, antidepressant activity and antistress activity were significantly improved.

On the other hand, it can be confirmed that in the case of MX7 and MX8, an additional synergistic effect can be exhibited. Therefore, it can be confirmed that, in the case of MX7 and MX8, very excellent antioxidant activity, antidepressant activity and antistress activity can be exhibited with a smaller content, and continuous administration or use for a long period of time is possible.

5. palatability test

The Lepidium extract (LME) and MX1 to MX9 were processed into a tea form, and each taster was asked to evaluate the preference based on flavor and taste. The tasters were asked to evaluate the palatability of MX1 to MX9 on an index of 1 to 10 by comparing them with the palatability of the Lepidium extract by 10 people who evaluated the gastric stress relieving effect. The evaluated result values were taken as an average and are shown in [Table 4] below. The following evaluation values were rounded off with a decimal point, and the higher the number, the better the palatability.

LME MX1 MX2 MX3 MX4 MX5 MX6 MX7 MX8 MX9 flavor 5 5 4 4 4 2 4 8 8 7 taste 5 4 5 5 3 2 5 7 8 6

(Unit: Index)

Referring to [Table 4], it can be seen that in the case of MX1 to MX5, a similar or lower degree of palatability compared to LME appears. On the other hand, in the case of MX7 to MX9, it can be seen that the effect of improving flavor and taste appears, and in particular, the degree of improvement in flavor and taste of MX7 and MX8 is excellent.

Although preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements by those skilled in the art using the basic concept of the present invention as defined in the following claims are also provided. is within the scope of the

Claims (7)

Contains horseradish extract as an active ingredient,
As to further include red pepper leaf extract, sagebrush extract, chazugi extract, Lamiaceae extract, red snow tea extract and cheonmundong extract,
Based on 100 parts by weight of the Lepidium extract, 0.1 to 20 parts by weight of a red pepper leaf extract, 5 to 50 parts by weight of an extract, 0.1 to 20 parts by weight of an extract, 0.1 to 10 parts by weight of an extract of Lamiaceae, 0.1 to 10 parts by weight of a red snow tea extract and 0.1 to 10 parts by weight of Cheonmundong extract
A composition for antistress. The method of claim 1,
wherein the horseradish extract is extracted using an extraction solvent selected from the group consisting of water, C1 to C6 lower alcohols, and mixtures thereof.
A composition for antistress. The method of claim 1,
That the composition exhibits an antidepressant effect
A composition for antistress. delete A fragrance for improving stress comprising the composition for antistress according to any one of claims 1 to 3. A food composition for preventing depression comprising the composition for anti-stress according to any one of claims 1 to 3. A pharmaceutical composition for preventing and treating depression comprising the composition for antistress according to any one of claims 1 to 3.

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