JP4628604B2 - Skin preparation - Google Patents

Description

【００４０】
実験例２ ヒスタミン遊離抑制作用
雄性Ｓｐｒａｑｕｅ−Ｄａｗｌｅｙ系ラットの腹腔内から採取した肥満細胞を用いてヒスタミン遊離抑制作用を測定した。すなわち、１μｇ／ｍＬのコンパウンド４８／８０による肥満細胞からヒスタミンの遊離を抑制する作用を遊離抑制率として求めた。肥満細胞はＳｕｌｌｉｖａｎらの方法（Ｊ．Ｉｍｍｕｎｏｌｏｇｙ，１１４，１４７３，１９７５）で採取し、ヒスタミンの定量はＭａｙらの方法（Ｊ．Ａｌｌｅｒｇｙ，４６，１２，１９７０）で行った。なお、陽性対照としてインドメタシンを用いた。
【００４１】
これらの実験結果を表２に示した。その結果、黒霊芝の熱水抽出物は霊芝（赤芝）の熱水抽出物と比較して優れたヒスタミン遊離抑制作用を示した。更に、黒霊芝と霊芝（赤芝）の熱水抽出物を併用した場合、顕著なヒスタミン遊離抑制作用を示した。また、その他の製造例の抽出物についても同様に優れたメラニン生成抑制作用を示した。
【００４２】
【表２】

【００４３】
実験例３ 使用試験１
実施例１〜３のクリーム、比較例１及び２の従来のクリームを用いて、シミ、ソバカスに悩む女性３０人（２１〜４６才）を対象に１ヶ月間の使用試験を行った。使用後、シミ、ソバカスの改善効果をアンケートにより判定した。
【００４４】
これらの試験結果を表３に示した。その結果、実施例１〜３で得られるクリームを用いた場合は、比較例１及び２で得られるクリームを用いた場合よりも、肌のシミ、ソバカスの改善作用を示した。すなわち、黒霊芝の熱水抽出物を含有するクリーム及び黒霊芝と霊芝（赤芝）の熱水抽出物を併用したクリームに顕著な作用を示した。なお、試験期間中、皮膚トラブルは一人もなく、安全性においても問題なかった。また、処方成分の劣化についても問題なかった。
【００４５】
【表３】

【００４６】
実験例４ 使用試験２
施例１〜３のクリーム、比較例１及び２の従来のクリームを用いて、肌荒れ、乾燥に悩む女性３０人（１９〜４８才）を対象に１ヶ月間の使用試験を行った。
使用後、肌荒れ、乾燥の改善効果をアンケートにより判定した。
【００４７】
これらの試験結果を表４に示した。その結果、実施例１〜３で得られるクリームを用いた場合は、比較例１及び２で得られるクリームを用いた場合よりも、肌荒れ、乾燥の改善作用を示した。すなわち、黒霊芝の熱水抽出物を含有するクリーム及び黒霊芝と霊芝（赤芝）の熱水抽出物を併用したクリームに顕著な作用を示した。なお、試験期間中、皮膚トラブルは一人もなく、安全性においても問題なかった。また、処方成分の劣化についても問題なかった。
【００４８】
【表４】

【００４９】
実験例５ 使用試験３
施例１〜３のクリーム、比較例１及び２の従来のクリームを用いて、シワ、タルミに悩む女性３０人（３０〜５８才）を対象に１ヶ月間の使用試験を行った。
使用後、シワ、タルミの改善効果をアンケートにより判定した。
【００５０】
これらの試験結果を表５に示した。その結果、実施例１〜３で得られるクリームを用いた場合は、比較例１及び２で得られるクリームを用いた場合よりも、シワ、タルミの改善作用を示した。すなわち、黒霊芝の熱水抽出物を含有するクリーム及び黒霊芝と霊芝（赤芝）の熱水抽出物を併用したクリームに顕著な作用を示した。なお、試験期間中、皮膚トラブルは一人もなく、安全性においても問題なかった。また、処方成分の劣化についても問題なかった。
【００５１】
【表５】

【００５２】
実施例４〜１１についても同様に使用試験を行ったところ、安全であり、優れたシミ、ソバカス、肌荒れ、乾燥、シワ、タルミ等の改善作用を示した。
【００５３】
【発明の効果】
以上のことから、本発明の黒霊芝の抽出物は、霊芝（赤芝）の抽出物と比較して特に優れたメラニン生成抑制作用及びヒスタミン遊離抑制作用をもち、安定性においても優れていた。さらに、黒霊芝及び霊芝（赤芝）抽出物を併用することにより、相乗効果を示した。また、これらの抽出物を含有する皮膚外用剤は、安全で優れた老化防止、美白及び抗炎症作用を示した。[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a skin external preparation particularly excellent in whitening, anti-inflammatory and anti-aging effects by containing an extract of black ganoderma.
[0002]
[Prior art]
In general, pigmentation of the skin seen in spots, buckwheat, sunburn, etc. is caused by excessive melanin pigment formation by melanin-producing cells present in the skin due to hormonal abnormalities or stimulation of ultraviolet rays, which deposits in the skin. Is considered to be the cause. As one method for preventing such pigmentation, a method for suppressing excessive production of melanin is known.
[0003]
As a cause of rough skin, there is skin inflammation due to dryness or ultraviolet rays. These inflammations eliminate skin firmness and cause wrinkles and tarmi. Therefore, an external preparation for skin that suppresses inflammation and improves rough skin is desired.
[0004]
Conventionally, as an effective cosmetic agent for improving such rough skin, spots, freckles, firmness, wrinkles, and tarmi, blended with extract of Ganoderma lucidum as disclosed in JP-A-58-113118 and JP-A-05-194175 The cosmetics that were made were known. Mannentake used in these cosmetics is a basidiomycete used in the herbal medicine “Ganoderma”, belongs to the family Ganodermaaceae, Ganoderma, Ganoderma (Akashiba), Black Ganoderma (Kuroshiba) ) And purple reishi (purple turf). However, in these publications, they are only used as garlic mushrooms, and Ganoderma, Black Ganoderma, and Purple Ganoderma are not compared in detail.
[0005]
Also, among Ganoderma, Ganoderma, Black Ganoderma, Purple Ganoderma are named simply because their fruit bodies are red, black, purple. Researched separately from Ganoderma and Purple Ganoderma. For example, according to the literature [Japan Brewing Association Journal, 85 (6), 385-392, 1990], black ganoderma does not contain the typical triterpene component contained in ganoderma or purple ganoderma, and tastes completely. Reported differently. From the above, there is room for further research on the effects of Ganoderma, Black Ganoderma, Purple Ganoderma, etc. on the skin.
[Problems to be solved by the invention]
In view of the above, a skin external preparation that is safe and excellent in stability, and has excellent whitening, anti-inflammatory and anti-aging effects is desired.
[0006]
[Means for Solving the Problems]
Under these circumstances, as a result of intensive studies, the present inventors have found that the extract of black ganoderma has particularly excellent melanin production inhibitory action and histamine release inhibitory action, and is also excellent in stability. I found out. Furthermore, it has been found that the combined use of black ganoderma and ganoderma (red turf) is more synergistic than the use of black ganoderma alone. Moreover, it discovered that the skin external preparation containing these extracts was safe and stable, and was excellent in the whitening, anti-inflammatory, and anti-aging action, and came to complete this invention.
[0007]
The black ganoderma (Kuroshiba) and the ganoderma (Akashiba) used in the present invention are basidiomycetes used in the herbal medicine “Ganoderma lucidum”, belong to the genus Mannenaceae and the genus Mannentake, and are a kind of Mannentake. According to the literature [Acta Microbiological Sinica, 19 (3), 265-279, 1979], the scientific name is classified into Kuro Ganoderma (Koshiba, scientific name: Ganoderma arum), Ganoderma (Akashiba, scientific name: Ganoderma lucidum). However, the classification method is not limited to this document. As the black ganoderma and ganoderma (red turf) used in the present invention, those widely distributed in China and the Japanese market can be used.
[0008]
The extract of black ganoderma or ganoderma (Akashiba) used in the present invention can be extracted from fruit bodies, mycelia, mycelium cultures, and the like. Preferably, an extract from a fruit body is good. The extraction method is not particularly limited, and for example, it may be a heat extraction or a room temperature extraction. The mycelium or mycelium culture can be produced by a conventional method.
[0009]
Examples of the solvent to be extracted include water, lower alcohols (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), liquid polyhydric alcohols (1,3-butylene glycol, propylene glycol). , Glycerin, etc.), ketones (acetone, methyl ethyl ketone, etc.), acetonitrile, esters (ethyl acetate, butyl acetate, etc.), hydrocarbons (hexane, heptane, liquid paraffin, etc.), ethers (ethyl ether, tetrahydrofuran, propyl ether) Etc.). Preferred are polar solvents such as water, lower alcohols and liquid polyhydric alcohols, and particularly preferred are water, ethanol, 1,3-butylene glycol and propylene glycol. These solvents may be used alone or in combination of two or more.
[0010]
The extract may be used as it is, or may be used after concentration, dilution, filtration, decolorization with activated carbon, deodorization, or the like, if necessary. Further, the extracted solution may be subjected to a treatment such as concentration to dryness, spray drying, freeze drying, etc. and used as a dried product, or the active ingredient may be concentrated or isolated by performing column purification or the like.
[0011]
In the external preparation for skin of the present invention, the above extract may be used as it is, and within the range not impairing the effect of the extract, oils and fats, waxes, hydrocarbons, fatty acids which are components used in the external preparation , Alcohols, esters, surfactants, metal soaps, pH adjusters, preservatives, fragrances, moisturizers, powders, UV absorbers, thickeners, pigments, antioxidants, whitening agents, chelating agents, etc. These ingredients can also be blended.
[0012]
The skin external preparation of the present invention can be used for any of cosmetics, quasi drugs, and pharmaceuticals. Examples of the dosage form include skin lotions, creams, emulsions, gels, aerosols, essences, packs, Examples of the cleaning agent, bath preparation, foundation, dusting powder, lipstick, ointment, and poultice applied to the skin.
[0013]
The amount of the extract used in the present invention is 0.0001% by weight or more, preferably 0.001 to 10% by weight in terms of solid matter, based on the total amount of the external preparation for skin of the present invention. If it is less than 0.0001% by weight, a sufficient effect is hardly expected. When it exceeds 10% by weight, the effect is hardly increased, which is uneconomical. In addition, the addition method may be added in advance or during the production, and may be appropriately selected in consideration of workability.
[0014]
Of the above extracts used in the present invention, a combination of black ganoderma and ganoderma (Akashiba) extract exhibits synergistic whitening, anti-inflammatory and anti-aging effects on the skin. The blend ratio of the extract of black ganoderma and ganoderma (red turf) is 5:95 to 95: 5, preferably 10:90 to 90:10 in terms of the weight ratio of the solid.
[0015]
【Example】
Next, in order to describe the present invention in detail, examples of producing the extract used in the present invention, formulation examples and experimental examples of the present invention will be given as examples, but the present invention is not limited thereto. The part of the amount shown in the examples means part by weight, and% means% by weight.
[0016]
Production Example 1 Black Ganoderma Hot Water Extract 400 g of purified water was added to 20 g of dried ganoderma fruit body, extracted at 95-100 ° C. for 2 hours, filtered, and the filtrate was concentrated and freeze-dried. 1.4 g of hot water extract of black reishi was obtained.
[0017]
Production Example 2 Black Ganoderma Ethanol Extract 100 g of dried ganoderma turf was added with 2 liters of ethanol, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness. 1.6g was obtained.
[0018]
Production Example 3 50% 1,3-butylene glycol aqueous extract of black ganoderma 1 L of purified water and 1 L of 1,3-butylene glycol were added to 100 g of dried black ganoderma and extracted for 7 days at room temperature, followed by filtration. 1.9 kg of a 50% 1,3-butylene glycol aqueous extract of black reishi was obtained.
[0019]
Production Example 4 Hot Water Extract of Ganoderma (Akashiba) 400 mL of purified water was added to 20 g of dried ganoderma (Akashiba), extracted at 95-100 ° C. for 2 hours, filtered, and the filtrate was concentrated and frozen. After drying, 2.0 g of hot water extract of Reishi (Akashiba) was obtained.
[0020]
Production Example 5 Ganoderma (Akashiba) Ethanol Extract 2 g ethanol was added to 100 g of dried ganoderma (Akashiba), extracted for 7 days at room temperature, filtered, and the filtrate was concentrated to dryness. 2.7 g of (Akashiba) ethanol extract was obtained.
[0021]
Production Example 6 50% 1,3-butylene glycol aqueous extract of Ganoderma (Akashiba) 1 L of purified water and 1 L of 1,3-butylene glycol were added to 100 g of dried ganoderma (Akashiba) and extracted at room temperature for 7 days. Thereafter, the mixture was filtered to obtain 1.9 kg of a 50% 1,3-butylene glycol aqueous solution extract of Reishi (Akashiba).
[0022]
Production Example 7 Extract of fungus body culture of black ganoderma cultivated using a jar fermenter. That is, 50 g of malt extract, 20 g of yeast extract, and 20 g of glucose were mixed with 5 L of purified water, placed in a jar, and then sterilized at 121 ° C. for 30 minutes. This was attached to a jar fermenter and inoculated with 150 mL of Kuro Reishi seed mycelium culture cultured in the same medium with shaking at 25 ° C. for 14 days. 300r. p. The mixture was cultured at 25 ° C. for 14 days with aeration of 3 L / min with aeration of m. The obtained culture was filtered to obtain 2.0 kg (wet weight) of mycelia.
2 L of purified water was added to the separated mycelium and extracted at 95-100 ° C. for 2 hours, followed by filtration. The filtrate was concentrated and freeze-dried to obtain 22 g of an extract of the fungus body culture of Kuro Reishi. .
[0023]
Example 1 Cream 1
Formulation Formulation 1. Black Reishi hot water extract (Production Example 1) 0.5 part2. Squalane 5.5
3. Olive oil 3.0
4). Stearic acid 2.0
5. Beeswax 2.0
6). Octyldodecyl myristate 3.5
7). Polyoxyethylene cetyl ether (20E.O.) 3.0
8). Behenyl alcohol 1.5
9. Glycerol monostearate2.5
10. Fragrance 0.1
11.1,3-butylene glycol 8.5
12 Ethyl paraoxybenzoate 0.05
13. Methyl paraoxybenzoate 0.2
14 [Manufacturing method] Components 2 to 9 are heated and dissolved and mixed with purified water to a total amount of 100, and kept at 70 ° C to obtain an oil phase. Ingredients 1 and 11 to 14 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring. The component 10 is added at 45 ° C., and further cooled to 30 ° C. to obtain a product.
[0024]
Example 2 Cream 2
In Example 1, 0.5 part of the hot water extract of black ganoderma 0.25 parts of the hot water extract of black ganoderma and the hot water extract of ganoderma (Akashiba) (Production Example 4) 0.25 The cream 2 was replaced with the part.
[0025]
Example 3 Cream 3
In Example 1, the hot water extract of black reishi was replaced with an extract of mycelium culture of black reishi (Production Example 7) to make Cream 3.
[0026]
Comparative Example 1 Conventional cream 1
In Example 1, the conventional cream 1 was obtained by replacing the hot water extract of black ganoderma with purified water.
[0027]
Comparative Example 2 Conventional Cream 2
In Example 1, the conventional cream 2 was obtained by replacing the hot water extract of black ganoderma by the hot water extract of Reishi (Akashiba) (Production Example 4).
[0028]
Example 4 Lotion Formulation Formulation 1. 10.0 parts of 50% 1,3-butylene glycol aqueous extract of black ganoderma (Production Example 3)
2. 1,3-butylene glycol 8.0
3. Glycerin 2.0
4). Xanthan gum 0.02
5. Citric acid 0.01
6). Sodium citrate 0.1
7). Ethanol 5.0
8). Methyl paraoxybenzoate 0.1
9. Polyoxyethylene hydrogenated castor oil (40E.O.) 0.1
10. Perfume proper amount11. [Manufacturing method] Components 1 to 6 and 11 and components 7 to 10 are uniformly dissolved in purified water, and both are mixed and filtered to obtain a product.
[0029]
Example 5 Latex 1
Formulation Formulation 1. Black Reishi lawn ethanol extract (Production Example 2) 0.5 part2. Squalane 5.0
3. Olive oil 5.0
4). Jojoba oil 5.0
5. Cetanol 1.5
6). Glycerol monostearate 2.0
7). Polyoxyethylene cetyl ether (20E.O.) 3.0
8). Polyoxyethylene sorbitan monooleate 2.0
(20 EO)
9. Fragrance 0.1
10. Propylene glycol 1.0
11. Glycerin 2.0
12 Methyl paraoxybenzoate 0.2
13. [Manufacturing method] Components 2 to 8 are heated and dissolved and mixed with purified water to a total amount of 100, and kept at 70 ° C to obtain an oil phase. Ingredients 1 and 10-13 are dissolved by heating and mixed, and kept at 75 ° C. to obtain an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring. The component 9 is added at 45 ° C., and further cooled to 30 ° C. to obtain a product.
[0030]
Example 6 Latex 2
In Example 5, 0.5 part of Kuro Ganoderma ethanol extract was added to 0.25 part of Kuro Ganoderma hot water extract (Production Example 1) and Ganoderma (Akashiba) hot water extract (Production Example 4). The emulsion 2 was replaced with 0.25 part.
[0031]
Example 7 Gel formulation formulation 0.1 parts of 50% 1,3-butylene glycol aqueous extract of black ganoderma (Production Example 3)
2. Ethanol 5.0
3. Methyl paraoxybenzoate 0.1
4). Polyoxyethylene hydrogenated castor oil (60 EO) 0.1
5. Perfume proper amount 6.1,3-butylene glycol 5.0
7). Glycerin 5.0
8). Xanthan gum 0.1
9. Carboxyvinyl polymer 0.2
10. Potassium hydroxide 0.2
11. [Production method] Ingredients 2 to 5 and ingredients 1 and 6 to 11 are uniformly dissolved in purified water, and the two are mixed to obtain a product.
[0032]
Example 8 Pack formulation Black Reishi hot water extract (Production Example 1) 0.1 part2. Reishi (Akashiba) ethanol extract (Production Example 5) 0.1
3. Polyvinyl alcohol 12.0
4). Ethanol 5.0
5.1,3-Butylene glycol 8.0
6). Methyl paraoxybenzoate 0.2
7). Polyoxyethylene hydrogenated castor oil (20 EO) 0.5
8). Citric acid 0.1
9. Sodium citrate 0.3
10. Perfume proper amount11. [Production Method] Components 1 to 11 are uniformly dissolved in purified water to make a total amount of 100 to obtain a product.
[0033]
Example 9 Foundation formula 1.0 part of 50% 1,3-butylene glycol aqueous extract of Kuro Reishi (Production Example 3)
2. Stearic acid 2.4
3. Polyoxyethylene sorbitan monostearate 1.0
(20 EO)
4). Polyoxyethylene cetyl ether (20E.O.) 2.0
5. Cetanol 1.0
6). Liquid lanolin 2.0
7). Liquid paraffin 3.0
8). Isopropyl myristate 6.5
9. Butyl paraoxybenzoate 0.1
10. Sodium carboxymethylcellulose 0.1
11. Bentonite 0.5
12 Propylene glycol 4.0
13. Triethanolamine 1.1
14 Methyl paraoxybenzoate 0.2
15. Titanium dioxide 8.0
16. Talc 4.0
17. Bengala 1.0
18. Yellow iron oxide 2.0
19. Perfume proper amount20. [Manufacturing method] Components 2 to 9 are heated and dissolved in purified water to a total amount of 100, and kept at 80 ° C to obtain an oil phase. Swell component 10 well with component 20, then add components 1 and 11-14 and mix uniformly. To this, components 15 to 18 pulverized and mixed with a pulverizer are added, and the mixture is stirred with a homomixer and kept at 75 ° C. to obtain an aqueous phase. The oily phase is added to the aqueous phase with stirring, cooled, and component 19 is added at 45 ° C, and cooled to 30 ° C with stirring to give a product.
[0034]
Example 10 Bath preparation formulation Black Reishi hot water extract (Production Example 1) 1.0 part2. Sodium bicarbonate 50.0
3. Yellow No. 202 (1) Appropriate amount 4. Perfume appropriate amount 5. [Production method] Ingredients 1-5 are uniformly mixed with anhydrous sodium sulfate to make a total amount of 100 to obtain a product.
[0035]
Example 11 Ointment Formulation Formulation 1. Ethanol extract of black reishi (Production Example 2) 1.0 part2. Polyoxyethylene cetyl ether (30E.O.) 2.0
3. Glycerol monostearate 10.0
4). Liquid paraffin 5.0
5. Cetanol 6.0
6). Methyl paraoxybenzoate 0.1
7). Propylene glycol 10.0
8). [Manufacturing method] Components 2-5 are heated and dissolved and mixed with purified water to 100, and kept at 70 ° C. to obtain an oil phase. Ingredients 1 and 6 to 8 are dissolved by heating and mixed, and kept at 75 ° C. to obtain an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled to 30 ° C. with stirring to obtain a product.
[0036]
Next, experimental examples will be given to explain the effects of the present invention in detail.
[0037]
Experimental Example 1 Melanin Production Inhibition Test Using B16 Mouse Melanoma A melanoma in the logarithmic growth phase was seeded with 3 × 10 ⁴ cells in a 60 mm petri dish, and Eagles'MEM (10% fetal bovine) containing samples of various concentrations Serum-containing) was added and cultured at 37 ° C. under 5% CO ₂ conditions. After 5 days of culture, the cells were detached from the petri dish, and the cells were ultrasonically disrupted. Then, a 2N aqueous sodium hydroxide solution was added, and the mixture was treated at 60 ° C. for 2 hours. D. 475 nm was measured. In addition, the cell lysate after sonication is subjected to protein quantification by the Lowry method (J. Biol. Chem., 193, 265-275, 1951), and the amount of melanin per amount of protein is compared, thereby suppressing melanin production. It was used as an index of action. Ascorbic acid was used as a positive control.
[0038]
The results of these experiments are shown in Table 1. As a result, the hot water extract of black ganoderma showed superior melanin production inhibitory action compared with the hot water extract of ganoderma (Akashiba). Furthermore, when the hot water extract of black ganoderma lucidum and the hot water extract of ganoderma (Akashiba) were used together, a remarkable melanin production inhibitory effect was shown. Moreover, the extract of other manufacture examples showed the outstanding melanin production inhibitory effect similarly.
[0039]
[Table 1]

[0040]
Experimental Example 2 Histamine Release Inhibitory Action Histamine release inhibitory action was measured using mast cells collected from the abdominal cavity of male Sprague-Dawley rats. That is, the effect of inhibiting the release of histamine from mast cells by 1 μg / mL compound 48/80 was determined as the release inhibition rate. Mast cells were collected by the method of Sullivan et al. (J. Immunology, 114, 1473, 1975), and histamine was quantified by the method of May et al. (J. Allergy, 46, 12, 1970). Indomethacin was used as a positive control.
[0041]
The results of these experiments are shown in Table 2. As a result, the hot water extract of black ganoderma showed superior histamine release inhibitory action compared with the hot water extract of ganoderma (Akashiba). Furthermore, when the hot water extract of black ganoderma and ganoderma (Akashiba) was used in combination, it showed a remarkable histamine release inhibitory action. Moreover, the extract of other manufacture examples showed the outstanding melanin production inhibitory effect similarly.
[0042]
[Table 2]

[0043]
Experimental example 3 Use test 1
Using the creams of Examples 1 to 3 and the conventional creams of Comparative Examples 1 and 2, a one-month use test was conducted on 30 women (21 to 46 years old) who suffer from spots and freckles. After use, the effect of improving spots and freckles was judged by a questionnaire.
[0044]
These test results are shown in Table 3. As a result, when the creams obtained in Examples 1 to 3 were used, the effect of improving skin spots and buckwheat was shown as compared with the case where the creams obtained in Comparative Examples 1 and 2 were used. That is, it showed a remarkable effect on the cream containing the hot water extract of black ganoderma and the cream containing the hot water extract of black ganoderma and ganoderma (Akashiba). During the test period, there was no skin problem and there was no problem with safety. There was also no problem with the deterioration of the prescription ingredients.
[0045]
[Table 3]

[0046]
Experiment 4 Use test 2
Using the creams of Examples 1 to 3 and the conventional creams of Comparative Examples 1 and 2, a one month use test was conducted on 30 women (19 to 48 years old) who suffer from rough skin and dryness.
After use, the improvement effect of rough skin and dryness was judged by a questionnaire.
[0047]
The test results are shown in Table 4. As a result, when the creams obtained in Examples 1 to 3 were used, the skin roughening and drying were improved as compared with the case where the creams obtained in Comparative Examples 1 and 2 were used. That is, it showed a remarkable effect on the cream containing the hot water extract of black ganoderma and the cream containing the hot water extract of black ganoderma and ganoderma (Akashiba). During the test period, there was no skin problem and there was no problem with safety. There was also no problem with the deterioration of the prescription ingredients.
[0048]
[Table 4]

[0049]
Experiment 5 Use test 3
Using the creams of Examples 1 to 3 and the conventional creams of Comparative Examples 1 and 2, a use test for one month was conducted on 30 women (30 to 58 years old) who suffer from wrinkles and tarmi.
After use, the effect of improving wrinkles and tarmi was judged by a questionnaire.
[0050]
The test results are shown in Table 5. As a result, when the creams obtained in Examples 1 to 3 were used, the wrinkles and tarmi were improved more than when the creams obtained in Comparative Examples 1 and 2 were used. That is, it showed a remarkable effect on the cream containing the hot water extract of black ganoderma and the cream containing the hot water extract of black ganoderma and ganoderma (Akashiba). During the test period, there was no skin problem and there was no problem with safety. There was also no problem with the deterioration of the prescription ingredients.
[0051]
[Table 5]

[0052]
Examples 4 to 11 were also tested in the same manner. As a result, they were safe and showed an excellent effect of improving stains, buckwheat, rough skin, dryness, wrinkles, tarmi, and the like.
[0053]
【The invention's effect】
From the above, the black ganoderma extract of the present invention has a particularly excellent melanin production inhibitory action and histamine release inhibitory action as compared with the ganoderma (Akashiba) extract, and is also excellent in stability. . Furthermore, the synergistic effect was shown by using black reishi and reishi (Akashiba) extract together. Moreover, the skin external preparation containing these extracts showed safe and excellent anti-aging, whitening and anti-inflammatory effects.

Claims (4)

A melanin production inhibitor characterized by containing an extract of black ganoderma . A histamine release inhibitor characterized by containing an extract of black reishi. A melanin production inhibitor comprising a black reishi extract and a reishi (Akashiba) extract. A histamine release inhibitor comprising a black reishi extract and a reishi (Akashiba) extract.