JP4473439B2 - Whitening agent - Google Patents

Description

【００２１】
実験例２ 使用試験
実施例１の化粧水及び実施例２の乳液を用いて、各々女性３０人（３０〜４５才）を対象に１ヶ月間の使用試験を行った。使用後、肌のしみ、そばかす及び透明感の改善に関するアンケート調査により皮膚の美白作用を判定した。その結果、実施例１の化粧水及び実施例２の乳液は、安全で優れた皮膚の美白作用を示した。
【００２２】
【発明の効果】
以上のことから、本発明のアグラセホの抽出物は優れたメラニン生成抑制作用をもち、優れた安定性を示した。さらに、アグラセホの抽出物は安全で優れた皮膚の美白作用を示した。 [0001]
BACKGROUND OF THE INVENTION
The present invention relates to a whitening agent containing an extract of a specific plant.
[0002]
[Prior art]
Skin pigmentation commonly found in spots, buckwheat, sunburn, etc., is caused by excessive melanin pigment formation in the skin by melanin-producing cells present in the skin due to hormonal abnormalities or stimulation of ultraviolet rays. Is considered to be the cause. As one method for preventing such pigmentation, a method for suppressing excessive production of melanin is known. Conventionally, hydroquinone, ascorbic acid (vitamin C), and the like have been used for the treatment of pigmentation.
[0003]
[Problems to be solved by the invention]
As a whitening agent, a highly safe natural raw material is desired rather than a chemically synthesized product such as hydroquinone which has a risk of side effects. Moreover, ascorbic acid is unstable and difficult to formulate.
[0004]
From the above, a whitening agent that is safe and excellent in stability is desired.
[0005]
[Means for Solving the Problems]
Under such circumstances, as a result of intensive studies, the present inventors have found that an extract of aglacefo has an excellent melanin production inhibitory effect and is also excellent in stability. Furthermore, the present inventors have found that a whitening agent containing an extract of agrasejo has a safe and excellent skin whitening action, and have completed the present invention.
[0006]
Agrasejo (scientific name: Berberis vulgaris, also known as: Gracejo, barberry) used in the present invention is a shrub of the Barberry family. It is widely distributed mainly in Europe, Central Asia and tropical America, and is widely used as a crude drug.
[0007]
The extract of aglacejo used in the present invention is extracted from a part of a plant body such as agrasefo leaves, stems, bark, flowers, fruits, roots or the whole plant. Preferably, those obtained by extraction from the leaves and stems of Agrasejo are good. The extraction method may be a room temperature extraction.
[0008]
As the solvent to be extracted, water or ethanol is preferable . A mixture of these solvents may be used.
[0009]
The Agrasejo extract may be used as it is, or may be used after concentration, dilution, filtration, or the like, if necessary. Further, the extracted solution may be subjected to a treatment such as concentration to dryness, spray drying, freeze drying, etc., and used as a dried product.
[0010]
In the whitening agent of the present invention, the above-mentioned extract may be used as it is, and within the range not impairing the effect of the extract of Agraceho, oils and fats, waxes, hydrocarbons which are components used in ordinary external preparations , Fatty acids, alcohols, esters, surfactants, metal soaps, pH adjusters, preservatives, fragrances, moisturizers, powders, UV absorbers, thickeners, pigments, antioxidants, whitening agents, Components such as chelating agents can also be blended.
[0011]
The whitening agent of the present invention can be used in any of cosmetics, quasi-drugs, and pharmaceuticals. Examples of the dosage form include lotions, creams, emulsions, gels, aerosols, ointments, poultices, Examples include essences, packs, cleaning agents, bath preparations, foundations, dusting powders, lipsticks, and the like applied to the skin.
[0012]
The blending amount of the extract of agrasefo used in the present invention is 0.0001% by weight or more, preferably 0.001 to 10% by weight, in terms of solid matter, with respect to the total amount of the preparation to be blended . If it is less than 0.0001% by weight, sufficient effects are hardly exhibited. When it exceeds 10% by weight, the effect is hardly increased, which is uneconomical. In addition, the addition method may be added in advance or during the production, and may be appropriately selected in consideration of workability.
[0013]
【Example】
Next, in order to describe the present invention in detail, examples of producing the extract used in the present invention, formulation examples and experimental examples of the present invention will be given as examples, but the present invention is not limited thereto. The part of the amount shown in the examples indicates part by weight, and% indicates% by weight.
[0014]
Production Example 1 50% ethanol extract of Aglacejo 1 L of purified water and 1 L of ethanol were added to 100 g of dried Agrasefo leaves and stems, extracted for 7 days at room temperature, filtered, and the filtrate was concentrated to dryness. 21 g of ethanol extract was obtained.
[0015]
Example 1 lotion formulation 1. 50 parts ethanol extract of Agrasejo ( Production Example 1 ) 0.1 part 1,3-butylene glycol 8.0
3. Glycerin 2.0
4). Xanthan gum 0.02
5). Citric acid 0.01
6). Sodium citrate 0.1
7). Ethanol 5.0
8). Methyl paraoxybenzoate 0.1
9. Polyoxyethylene hydrogenated castor oil (40E.O.) 0.1
10. Perfume proper amount11. [Manufacturing method] Components 1 to 6 and 11 and components 7 to 10 are uniformly dissolved in purified water, and both are mixed and filtered to obtain a product.
[0016]
Example 2 Emulsion Formulation Amount 0.5 parts of Agrasejo 50% ethanol extract ( Production Example 1 ) Squalane 5.0
3. Olive oil 5.0
4). Jojoba oil 5.0
5). Cetanol 1.5
6). Glycerol monostearate 2.0
7). Polyoxyethylene cetyl ether (20E.O.) 3.0
8). Polyoxyethylene sorbitan monooleate (20E.O.) 2.0
9. Fragrance 0.1
10. Propylene glycol 1.0
11. Glycerin 2.0
12 Methyl paraoxybenzoate 0.2
13. [Manufacturing method] Components 2 to 8 are heated and dissolved and mixed with purified water to a total amount of 100, and kept at 70 ° C to obtain an oil phase. Ingredients 1 and 10-13 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring.
[0017]
Next, experimental examples will be given to explain the effects of the present invention in detail.
[0018]
Experimental Example 1 Melanin production inhibition test using B16 mouse melanoma (whitening effect)
Logarithmically growing melanoma is seeded at 3 × 10 4 cells in a 60 mm dish and added with 50% ethanol extract of various concentrations of Aglaceho or Eagles' MEM (containing 10% fetal calf serum) containing ascorbic acid as a positive control. The cells were cultured at 37 ° C. under 5% CO ₂ conditions. Further, in order to confirm the stability of the sample, the same evaluation was performed using a solution in which the aqueous solution of the sample was allowed to stand at room temperature for 24 hours. After 5 days of culture, the cells were detached from the dish, and the cells were sonicated, and then treated with 2N aqueous sodium hydroxide at 60 ° C. for 2 hours. D. 475 nm was measured. In addition, the protein quantification is performed for the cell lysate after sonication by Lowry's method (J. Biol. Chem., 193, 265-275, 1951), and the amount of melanin per amount of protein is compared to suppress melanin production. It was used as an index of action.
[0019]
The test results are shown in Table 1. In addition, when ascorbic acid was allowed to stand for 24 hours, the inhibitory action on melanin production decreased, but the action of the extract of aglacefo did not change. As a result, it was confirmed that the extract of aglacefo had a stable and excellent melanin production inhibitory action.
[0020]
[Table 1]

[0021]
Experiment 2 Usage test
Using the lotion of Example 1 and the emulsion of Example 2, a use test for one month was conducted for 30 women (30 to 45 years old). After use, the skin whitening effect was determined by a questionnaire survey on the improvement of skin spots, freckles and transparency. As a result, the lotion of Example 1 and the emulsion of Example 2 showed a safe and excellent skin whitening effect.
[0022]
【The invention's effect】
From the above, the extract of aglacefo of the present invention had an excellent melanin production inhibitory action and showed excellent stability. In addition, Agrasejo extract showed a safe and excellent skin whitening effect.

Claims (1)

A whitening agent comprising an extract of water and / or ethanol of Agrasejo, wherein the extraction method is extraction at room temperature.