JP5154835B2 - Whitening cosmetics - Google Patents

Description

  The present invention relates to a whitening agent and a whitening cosmetic that can exhibit a whitening effect by suppressing the production of melanin that causes spots, buckwheat, skin pigmentation, and the like.

  In general, melanin changes from tyrosine to dopa and from dopa to dopaquinone by the action of tyrosinase biosynthesized in pigment cells, and is formed through intermediates such as 5,6-dihydroxyindophenol. ing. Spots, buckwheat, and skin pigmentation are thought to be caused by melanin pigment cells that are excessively produced in the skin due to hormonal abnormalities and stimulation of ultraviolet rays, which are deposited in the skin.

  Yen Ganoderma (Arenaria kansuensis Maxim.) Is a perennial dwarf herb that is widely distributed in China such as Tibet, Gansu, Qinghai, and Western Sichuan. Known to cure influenza, pneumonia, jaundice, rib pain, colic.

  Although there are cases where Yukigakushi extract is blended in topical skin preparations as a moisturizer, it is extremely effective for cosmetics containing Yukigakushiki extract that have the effect of inhibiting melanin production. It is not known at all that it has an excellent whitening effect.

  Conventionally, whitening cosmetics for the purpose of prevention and treatment of stains, buckwheat and the like have been mixed with placenta extracts such as L-ascorbic acid and its derivatives, hydroquinone derivatives, kojic acid pyrones, and placenta extract.

These substances are widely known as substances that have effects such as suppression of melanin production and light color bleaching of the produced melanin, and exhibit a whitening effect. However, when these substances are used alone, the storage stability is not sufficient, the effect is not exhibited, and there is a problem in safety, etc., which is not sufficient.
JP 2007-77079 A Japanese Patent No. 3229517 Shanghai Science and Technology Publishing Co., Ltd. Shogakukan “Chugakudai Dictionary 3” Shogakukan

  There has been a demand for the development of a whitening cosmetic composition derived from a natural product having an excellent effect capable of eliminating the above disadvantages.

  The present invention has found that an extract of snow ganoderma (Arenaria kansuensis Maxim.) Has a whitening effect, and further found that a cosmetic containing a snow ganoderma extract has an excellent whitening effect, thereby completing the present invention. It came to do.

  The extract of snow ganoderma used in the present invention is extracted from a part of the plant body such as leaves, stems, flowers, roots or the whole plant. Preferably, the one obtained by extracting from the whole plant plant is good. The extraction method is not particularly limited, and for example, it may be a heat extraction or a room temperature extraction.

  Examples of the solvent to be extracted include water, lower alcohols (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), liquid polyhydric alcohols (1,3-butylene glycol, propylene glycol). , Glycerin, etc.), ketones (acetone, methyl ethyl ketone, etc.), acetonitrile, esters (ethyl acetate, butyl acetate, etc.), hydrocarbons (hexane, heptane, liquid paraffin, etc.), ethers (ethyl ether, tetrahydrofuran, propyl ether) Etc.). Preferred are polar solvents such as water, lower alcohols and liquid polyhydric alcohols, and particularly preferred are water, ethanol, 1,3-butylene glycol and propylene glycol. These solvents may be used alone or in combination of two or more.

  The snow ganoderma extract may be used as it is, and may be used after concentration, dilution and filtration treatment, decolorization with activated carbon, deodorization treatment, or the like, if necessary. Further, the extracted solution may be subjected to a treatment such as concentration to dryness, spray drying, freeze drying, etc., and used as a dried product.

  The blending amount of the snow ganoderma extract is not particularly limited, but is 0.0001% by weight (hereinafter simply referred to as “%”) to 5% in terms of solids based on the total amount of the whitening cosmetic of the present invention, Preferably, 0.001% to 1% is blended. If it is less than 0.0001%, a sufficient effect may not be obtained, and if it is 5% or more, the appearance of the whitening cosmetic composition may be affected. In addition, the addition method may be added in advance or during the production, and may be appropriately selected in consideration of workability.

  In the whitening cosmetics of the present invention, optional components such as a physiologically active substance, a base material, and a stabilizing component are appropriately blended in addition to the snow ganoderma extract as long as the effects of the present invention are not impaired. be able to. Examples of such optional components include purified water, fats and oils, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, metal soaps, pH adjusters, preservatives, perfumes, moisturizers, Examples thereof include powders, ultraviolet absorbers, thickeners, dyes, antioxidants, whitening agents, chelating agents and the like.

  Examples of the dosage form of the whitening cosmetic of the present invention include skin lotion, cream, emulsion, gel, aerosol, essence, pack, cleaning agent, bath preparation, foundation, dusting, lipstick and the like. .

  The whitening cosmetic composition of the present invention is characterized by blending an extract of Arenaria kansuensis maxim. And exhibits an excellent whitening effect.

  Next, in order to describe the present invention in detail, examples of producing the extract used in the present invention, formulation examples and experimental examples of the present invention will be given as examples, but the present invention is not limited thereto. In the examples, the part of the amount is part by weight, and% is% by weight.

Production Example 1 Hot Water Extract of Snow Reishi Mushrooms Add 1500 ml of purified water to 75 g of Snow Reishi grass, extract at 95-100 ° C for 2 hours, filter, concentrate the filtrate, freeze-dry and snow 0.8 g of hot water extract of Ganoderma was obtained.

Production Example 2 Snow Ganoderma 30% Ethanol Extract To 75 g of snow ganoderma on the ground, 1500 mL of 30% ethanol is added, extracted at room temperature for 7 days, filtered, and the filtrate is concentrated to dryness. 2.4 g of 30% ethanol extract was obtained.

Production Example 3 Snow Ganoderma Ethanol Extract Add 1500 mL of ethanol to 75 g of snow ganoderma grass, extract for 7 days at room temperature, filter, concentrate the filtrate to dryness, and extract the ethanol extract of snow ganoderma. 3.1g was obtained.

Formulation Example 1 Lotion A
Formulation Formulation 1. Snow Ganoderma Hot Water Extract (Production Example 1) 0.1 part 2.1,3-butylene glycol 3.0
3. Glycerin 1.0
4). Xanthan gum 0.02
5. Citric acid 0.01
6). Sodium citrate 0.1
7). Ethanol 1.0
8). Methyl paraoxybenzoate 0.1
9. Polyoxyethylene hydrogenated castor oil (40E.O.) 0.1
10. Perfume proper amount11. [Manufacturing method] Components 1 to 6 and 11 and components 7 to 10 are uniformly dissolved in purified water, and both are mixed and filtered to obtain a product.

Comparative Example 1 Conventional lotion In Formulation 1, lotion B was obtained by replacing only the hot water extract of snow ganoderma with purified water.

Formulation Example 2 Cream A
Formulation Formulation 1. Snow Ganoderma 30% ethanol extract (Production Example 2) 0.1 part Squalane 5.5
3. Olive oil 3.0
4). Stearic acid 2.0
5. Beeswax 2.0
6). Octyldodecyl myristate 3.5
7). Polyoxyethylene cetyl ether (20E.O.) 3.0
8). Behenyl alcohol 1.5
9. Glycerol monostearate2.5
10. Fragrance 0.1
11. Methyl paraoxybenzoate 0.2
12 Ethyl paraoxybenzoate 0.05
13.1,3-Butylene glycol 8.5
14 [Manufacturing method] Components 2 to 9 are heated and dissolved and mixed with purified water to a total amount of 100, and kept at 70 ° C to obtain an oil phase. Ingredients 1 and 11 to 14 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring. The component 10 is added at 45 ° C., and further cooled to 30 ° C. to obtain a product.

Comparative Example 2 Conventional Cream In Formulation Example 2, cream B was obtained by replacing only 30% ethanol extract of snow ganoderma with purified water.

Formulation Example 3 Emulsion Formulation Formulation 1. Snow Reishi Hot Water Extract (Production Example 1) 0.5 part Squalane 5.0
3. Olive oil 5.0
4). Jojoba oil 5.0
5. Cetanol 1.5
6). Glycerol monostearate 2.0
7). Polyoxyethylene cetyl ether (20E.O.) 3.0
8). Polyoxyethylene sorbitan monooleate (20E.O.) 2.0
9. Perfume appropriate amount10. Propylene glycol 1.0
11. Glycerin 2.0
12 Methyl paraoxybenzoate 0.2
13. [Manufacturing method] Components 2 to 8 are heated and dissolved and mixed with purified water to a total amount of 100, and kept at 70 ° C to obtain an oil phase. Ingredients 1 and 10-13 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring. The component 9 is added at 45 ° C., and further cooled to 30 ° C. to obtain a product.

Formulation Example 4 Gel formulation Formulation amount 1. Snow Ganoderma Ethanol Extract (Production Example 3) 0.001 part Ethanol 5.0
3. Methyl paraoxybenzoate 0.1
4). Polyoxyethylene hydrogenated castor oil (60 EO) 0.1
5. Perfume appropriate amount 6.1,3-butylene glycol 5.0
7). Glycerin 5.0
8). Xanthan gum 0.1
9. Carboxyvinyl polymer 0.2
10. Potassium hydroxide 0.2
11. [Production method] Ingredients 1 to 5 and 6 to 11 are uniformly dissolved in purified water to 100, and both are mixed to obtain a product.

Formulation Example 5 Foundation Formulation Amount 0.05 parts of hot water extract of Snow Reishi (Production Example 1) Stearic acid 2.4
3. Polyoxyethylene sorbitan monostearate (20EO) 1.0
4). Polyoxyethylene cetyl ether (20E.O.) 2.0
5. Cetanol 1.0
6). Liquid lanolin 2.0
7). Liquid paraffin 3.0
8). Isopropyl myristate 6.5
9. Sodium carboxymethylcellulose 0.1
10. Bentonite 0.5
11. Propylene glycol 4.0
12 Triethanolamine 1.1
13. Methyl paraoxybenzoate 0.2
14 Titanium dioxide 8.0
15. Talc 4.0
16. Bengala 1.0
17. Yellow iron oxide 2.0
18. Perfume proper amount19. [Manufacturing method] Components 2 to 8 are heated and dissolved in purified water to a total amount of 100, and kept at 80 ° C to obtain an oil phase. Swell component 9 well in component 19, then add components 1 and 10-13 and mix uniformly. To this, components 14 to 17 pulverized and mixed with a pulverizer are added, and the mixture is stirred with a homomixer and kept at 75 ° C. to obtain an aqueous phase. The oil phase is added to this aqueous phase with stirring, cooled, component 18 is added at 45 ° C., and cooled to 30 ° C. with stirring to give a product.

  Next, experimental examples will be given to explain the effects of the present invention in detail.

Experimental Example 1 Melanin Production Inhibition Test Using B16 Mouse Melanoma A melanin production inhibitory effect was measured using a hot water extract of Snow Reishi (Production Example 1) and a 30% ethanol extract (Production Example 2) as samples.

B16 cells, a mouse melanoma-derived cell line, were seeded at 3 × 10 ⁴ cells in a 6 cm dish, and cultured for 5 days in MEM with NEAA containing 10% FBS under 5% CO ₂ and 37 ° C. conditions. At that time, each sample was added after adjusting the final concentration. Next, after the cells were washed twice with PBS (−), 1 mL of PBS (−) was added and collected with a rubber policeman. PBS (-) 0.5mL was added to the pellet obtained by centrifugation, and the pellet was dissolved by ultrasonic crushing operation. Protein quantification was performed using the Lowry method {Lowry, O .; H. et al. , J .; Biol. Chem. , 193, 265-275 (1951)}. When measuring the amount of melanin per mg protein, 0.5 mL of 4N sodium hydroxide was added to the remaining cell disruption solution taken for protein quantification and reacted at 60 ° C. for 2 hours. D. 475 was measured, and melanin was determined from the calibration curve. The data calculated the amount of melanin per 1 mg protein, the amount of melanin not added to the sample was taken as a control, and the inhibition rate of melanin generation was calculated from the value of the amount of melanin generated when the sample was added to the control.

  The results of these experiments are shown in Table 1. The snow ganoderma extract had a melanin production inhibitory effect, and the hot water extract showed a superior melanin production inhibitory effect.

Experimental Example 2 Use Test A woman suffering from spots and freckles using the lotion A of Formulation Example 1, the conventional lotion B of Comparative Example 1 and the cream A of Formulation Example 2 and the conventional cream B of Comparative Example 2. A 20-month use test was conducted for 20 people (21 to 46 years old). After use, the effect of improving spots and freckles was judged by a questionnaire.

  These test results are shown in Tables 2 and 3. Skin lotion A and cream A formulated with snow ganoderma extract showed excellent stain and buckwheat improvement effects. During the test period, there was no skin problem and there was no problem with safety. There was also no problem with the deterioration of the prescription ingredients.

  Formulation Examples 3 to 5 also had a good whitening effect.

Claims (2)

A whitening agent comprising a water extract of Arenaria kansuensis Maxim. A whitening cosmetic comprising the whitening agent according to claim 1 .