JP5184840B2 - Topical skin preparation - Google Patents

Description

  The present invention relates to a skin external preparation excellent in anti-aging and whitening action, characterized by containing an extract from the upper part of ogi.

  In recent years, free radicals and active oxygen have been taken up as factors that oxidize biological components, and their adverse effects have become a problem. Free radicals and active oxygen are said to be generated in vivo and decompose or crosslink biological tissues such as collagen, and oxidize lipids to produce lipid peroxides that damage cells. Such injuries are thought to cause aging such as wrinkles and firmness of the skin, and one method for preventing aging is to incorporate an antioxidant that removes free radicals and active oxygen. Are known. Conventionally, ascorbic acid, tocopherol, 3,5-tert-butyl-4-hydroxytoluene (BHT), superoxide dismutase (SOD) and the like have been used as free radical scavengers used for the purpose of preventing aging.

  In general, pigmentation of the skin seen in spots, buckwheat, sunburn, etc. is caused by excessive melanin pigment formation by melanin-producing cells present in the skin due to hormonal abnormalities or stimulation of ultraviolet rays, which deposits in the skin. Is considered to be the cause. As one method for preventing such pigmentation, a method for suppressing excessive production of melanin is known. Conventionally, treatments for external application of hydroquinone, ascorbic acid or the like have been performed for the treatment of pigmentation.

The roots of ogi are often used for traditional Chinese medicine and applied to cosmetics (Patent Document 1). However, the above-ground part of ogi has no application examples for cosmetics, and in particular, an antioxidant action and a melanin production inhibitory action have not been studied.
JP-A 61-263905

  SOD used for the purpose of preventing skin aging or anti-oxidation is unstable, difficult to formulate, and tocopherol is not effective enough. Moreover, since BHT etc. which are synthetic compounds have a problem in safety | security and there exists a restriction | limiting in compounding quantity, it is not a chemically synthesized product but the natural raw material which is stable and has few side effects, and is more effective is desired. Ascorbic acid used as a whitening agent has disadvantages such as being easily decomposed over time, and hydroquinone has safety problems, so it is safer, more stable, and more effective. The raw material of is desired.

  Under such circumstances, the present inventors have intensively studied, and as a result, have found that the extract of the above-ground part of ogi has an excellent anti-oxidant action and melanin production-inhibiting action and is also excellent in stability. Furthermore, it discovered that the skin external preparation containing the extract was safe and stable, and was excellent in anti-aging and whitening effect, and came to complete this invention.

  The sea urchin (Leguminosae) used in the present invention is Astragalus membranaceus (Astragalus membranaceus) and Astragalus mongolicus (Astragalus mongolicus), and the above-ground part can be used. The aerial part includes leaves, stems, flowers and fruits. The ground part of the ogi can be used for health foods.

  The extraction method of the extract of the above-ground ogi used for this invention is not specifically limited, For example, what was extracted by heating and normal temperature extraction may be used.

  Examples of the solvent to be extracted include water, lower alcohols (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), liquid polyhydric alcohols (1,3-butylene glycol, propylene glycol). , Glycerin, etc.), ketones (acetone, methyl ethyl ketone, etc.), acetonitrile, esters (ethyl acetate, butyl acetate, etc.), hydrocarbons (hexane, heptane, liquid paraffin, etc.), ethers (ethyl ether, tetrahydrofuran, propyl ether) Etc.). Preferred are polar solvents such as water, lower alcohols and liquid polyhydric alcohols, and particularly preferred are water, ethanol, 1,3-butylene glycol and propylene glycol. These solvents may be used alone or in combination of two or more.

  The extract may be used as it is, or may be used after concentration, dilution and filtration treatment, decolorization with activated carbon, deodorization treatment, or the like, if necessary. Further, the extracted solution may be subjected to a treatment such as concentration to dryness, spray drying, freeze drying, etc., and used as a dried product.

  In the external preparation for skin of the present invention, the above extract may be used as it is, and within the range not impairing the effect of the extract, oils and fats, waxes, hydrocarbons, fatty acids which are components used in the external preparation , Alcohols, esters, surfactants, metal soaps, pH adjusters, preservatives, fragrances, moisturizers, powders, UV absorbers, thickeners, pigments, antioxidants, whitening agents, chelating agents, etc. These components can be blended.

  The external preparation for skin of the present invention can be used for any of cosmetics, quasi drugs, and pharmaceuticals. Examples of the dosage form include skin lotions, creams, emulsions, gels, aerosols, essences, Examples include packs, cleaning agents, bath preparations, foundations, dusting powders, lipsticks, ointments, and poultices applied to the skin.

  The amount of the extract used in the present invention is 0.0001% by weight or more, preferably 0.001 to 10% by weight in terms of solid matter, based on the total amount of the external preparation for skin of the present invention. If it is less than 0.0001% by weight, a sufficient effect is hardly expected. When the blending amount exceeds 10% by weight, the effect is hardly recognized and it is uneconomical. In addition, the addition method may be added in advance or during the production, and may be appropriately selected in consideration of workability.

  The above-ground extract of the ogi of the present invention had a DPPH radical scavenging action and a melanin production-inhibiting action superior to the root extract, and was also excellent in stability. Furthermore, the skin external preparation containing the extract showed safe and excellent anti-aging and whitening action.

  Next, in order to describe the present invention in detail, examples of producing the extract used in the present invention, formulation examples and experimental examples of the present invention will be given as examples, but the present invention is not limited thereto. The part of the compounding amount shown in the prescription example means part by weight, and% means weight%.

Production Example 1 Hot water extract of above-ground part of ogi 400 ml of purified water was added to 20 g of dried product of above-ground part of ogi, extracted at 95-100 ° C. for 2 hours, filtered, and the filtrate was concentrated, freeze-dried and dried. 3.7g of hot water extract of the above-ground part was obtained.

Production Example 2 50% ethanol extract of above-ground part of ogi 400 mL of 50 (v / v)% aqueous ethanol solution was added to 20 g of dried product of above-ground part of ogi, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness. As a result, 2.6 g of 50% ethanol extract of the above-ground part of the ogi was obtained.

Production Example 3 50% 1,3-butylene glycol extract of the above-ground part of the ogi 200 g of purified water and 200 g of 1,3-butylene glycol were added to 20 g of the dried product of the above-ground part of the ogi, followed by extraction at room temperature for 7 days, followed by filtration. 360 g of 50% 1,3-butylene glycol extract of the above-ground part of ogi was obtained.

Comparative Production Example 1 Hot water extract of ogi roots 400 mL of purified water was added to 20 g of dried ogi roots (yellow jade), extracted at 95-100 ° C. for 2 hours, filtered, and the filtrate was concentrated and lyophilized. As a result, 4.5 g of hot water extract of oat root was obtained.

Comparative Production Example 2 50% Ethanol Extract of Ogi Root 400 mL of 50 (v / v)% ethanol aqueous solution was added to 20 g of dried dried ogi root (yellow jade), extracted at room temperature for 7 days, filtered, and the filtrate was filtered. Concentration to dryness yielded 5.1 g of 50% ethanol extract of oat root.

Formulation Example 1 Lotion Formulation Amount Hot water extract of above-ground ogi (Production Example 1) 0.1 part 2.1,3-butylene glycol 8.0
3. Glycerin 2.0
4). Xanthan gum 0.02
5. Citric acid 0.01
6). Sodium citrate 0.1
7). Ethanol 5.0
8). Methyl paraoxybenzoate 0.1
9. Polyoxyethylene hydrogenated castor oil (40E.O.) 0.1
10. Perfume
11. [Manufacturing method] Components 1 to 6 and 11 and components 7 to 10 are uniformly dissolved in purified water, and both are mixed and filtered to obtain a product.

Comparative Example 1 Conventional lotion In Formulation Example 1, the hot water extract of the above-ground part of Ogi (Production Example 1) was replaced with the hot water extract of ogi root (Comparative Production Example 1) to obtain a conventional lotion. .

Formulation Example 2 Cream Formulation Amount 50 parts ethanol extract of the above-ground part of ogi (Production Example 2) 0.05 part Squalane 5.5
3. Olive oil 3.0
4). Stearic acid 2.0
5. Beeswax 2.0
6). Octyldodecyl myristate 3.5
7). Polyoxyethylene cetyl ether (20E.O.) 3.0
8). Behenyl alcohol 1.5
9. Glycerol monostearate2.5
10. Fragrance 0.1
11. Methyl paraoxybenzoate 0.2
12 Ethyl paraoxybenzoate 0.05
13.1,3-Butylene glycol 8.5
14 [Manufacturing method] Components 2 to 9 are heated and dissolved and mixed with purified water to a total amount of 100, and kept at 70 ° C to obtain an oil phase. Ingredients 1 and 11 to 14 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring. The component 10 is added at 45 ° C., and further cooled to 30 ° C. to obtain a product.

Comparative Example 2 Conventional Cream In Formulation Example 2, a 50% ethanol extract of the above-ground part of Ogi (Production Example 2) was replaced with a 50% ethanol extract of Ogi root (Comparative Production Example 2) to obtain a conventional cream. .

Formulation Example 3 Emulsion Formulation Formulation 1. 1. Hot water extract of above-ground part of ogi (Production Example 1) 0.001 part Squalane 5.0
3. Olive oil 5.0
4). Jojoba oil 5.0
5. Cetanol 1.5
6). Glycerol monostearate 2.0
7). Polyoxyethylene cetyl ether (20E.O.) 3.0
8). Polyoxyethylene sorbitan monooleate (20E.O.) 2.0
9. Fragrance 0.1
10. Propylene glycol 1.0
11. Glycerin 2.0
12 Methyl paraoxybenzoate 0.2
13. Make the total volume 100 with purified water
[Manufacturing method] Components 2 to 8 are dissolved by heating and mixed, and kept at 70 ° C to obtain an oil phase. Ingredients 1 and 10-13 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring. The component 9 is added at 45 ° C., and further cooled to 30 ° C. to obtain a product.

Formulation Example 4 Gel formulation Formulation amount 1. 50% 1,3-butylene glycol extract of aboveground part of ogi (Production Example 3) 1.0 part Ethanol 5.0
3. Methyl paraoxybenzoate 0.1
4). Polyoxyethylene hydrogenated castor oil (60 EO) 0.1
5. Perfume
6.1,3-Butylene glycol 5.0
7). Glycerin 5.0
8). Xanthan gum 0.1
9. Carboxyvinyl polymer 0.2
10. Potassium hydroxide 0.2
11. [Production method] Ingredients 2 to 5 and ingredients 1 and 6 to 11 are uniformly dissolved in purified water, and the two are mixed to obtain a product.

Formulation Example 5 Pack Formulation Formulation 1. 1. Hot water extract from above-ground part of ogi (Production Example 1) 0.1 part 50% ethanol extract of above-ground part of ogi (Production Example 2) 0.1
3. Polyvinyl alcohol 12.0
4). Ethanol 5.0
5.1,3-Butylene glycol 8.0
6). Methyl paraoxybenzoate 0.2
7). Polyoxyethylene hydrogenated castor oil (20 EO) 0.5
8). Citric acid 0.1
9. Sodium citrate 0.3
10. Perfume proper amount11. [Production Method] Components 1 to 11 are uniformly dissolved in purified water to make a total amount of 100 to obtain a product.

Formulation Example 6 Foundation Formulation Amount 1. 50 parts of 50% ethanol extract of above-ground ogi (Production Example 2) Stearic acid 2.4
3. Polyoxyethylene sorbitan monostearate (20EO) 1.0
4). Polyoxyethylene cetyl ether (20E.O.) 2.0
5. Cetanol 1.0
6). Liquid lanolin 2.0
7). Liquid paraffin 3.0
8). Isopropyl myristate 6.5
9. Sodium carboxymethylcellulose 0.1
10. Bentonite 0.5
11. Propylene glycol 4.0
12 Triethanolamine 1.1
13. Methyl paraoxybenzoate 0.2
14 Titanium dioxide 8.0
15. Talc 4.0
16. Bengala 1.0
17. Yellow iron oxide 2.0
18. Perfume proper amount19. [Manufacturing method] Components 2 to 8 are heated and dissolved in purified water to a total amount of 100, and kept at 80 ° C to obtain an oil phase. Swell component 9 well in component 19, then add components 1 and 10-13 and mix uniformly. To this, components 14 to 17 pulverized and mixed with a pulverizer are added, and the mixture is stirred with a homomixer and kept at 75 ° C. to obtain an aqueous phase. The oil phase is added to this aqueous phase with stirring, cooled, component 18 is added at 45 ° C., and cooled to 30 ° C. with stirring to give a product.

Formulation Example 7 Bath preparation formulation 1. Hot water extract of above-ground part of ogi (Production Example 1) 5.0 parts Sodium bicarbonate 50.0
3. Yellow No. 202 (1) Appropriate amount 4. Perfume appropriate amount 5. [Manufacturing method] Ingredients 1 to 5 are mixed uniformly with sodium sulfate to make a product.

Formulation Example 8 Ointment Formulation Formulation 1. 1. Hot water extract from above-ground part of ogi (Production Example 1) 0.01 part 50% 1,3-butylene glycol extract of aboveground part of ogi (Production Example 3) 0.5
3. Polyoxyethylene cetyl ether (30E.O.) 2.0
4). Glycerol monostearate 10.0
5. Liquid paraffin 5.0
6). Cetanol 6.0
7). Methyl paraoxybenzoate 0.1
8). Propylene glycol 10.0
9. [Production Method] Components 3 to 6 are heated and dissolved and mixed with purified water to a total amount of 100, and kept at 70 ° C. to obtain an oil phase. Ingredients 1, 2, and 7-9 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled to 30 ° C. with stirring to obtain a product.
Next, experimental examples will be given to explain the effects of the present invention in detail.

Experimental Example 1 Antioxidant action (DPPH radical scavenging action)
Using Production Examples 1 and 2 and Comparative Production Examples 1 and 2 as samples, the scavenging action of DPPH radical, which is a kind of free radical, was measured. As a control, ascorbic acid was used. Further, in order to confirm the stability of the sample, the sample was stored at 40 ° C. for 2 weeks, and then the same measurement was performed.

To 0.3 mL of the sample aqueous solution of each concentration, add 0.1 mL of acetate buffer (pH 5.5), 0.4 mL of ethanol, and 0.2 mL of 0.5 mM DPPH (diphenylcyclic hydrazyl) ethanol solution at 37 ° C. for 30 minutes. After the reaction, the absorbance at a wavelength of 517 nm was measured. The erasure rate of each sample was calculated by the following formula.
Erase rate (%) = (1-sample absorbance / blank absorbance) × 100
The test results are shown in Table 1. Ascorbic acid significantly decreased the DPPH radical scavenging action by storage at 40 ° C. for 2 weeks, but the extract of the above-ground part of ogi did not change the scavenging action. Moreover, the effect was higher than that of the extract of oat root. From the above, the extract of the above-ground part of ogi showed a stable and excellent DPPH radical scavenging action.

Experimental Example 2 Melanin Production Inhibition Test Using B16 Mouse Melanoma Using Production Examples 1 and 2 and Comparative Production Examples 1 and 2 as samples, melanin production inhibitory action was measured.

B16 cells, a mouse melanoma-derived cell line, were seeded at 3 × 10 ⁴ cells in a 6 cm dish, and cultured for 5 days in MEM with NEAA containing 10% FBS under 5% CO ₂ and 37 ° C. conditions. At that time, each sample was added after adjusting the final concentration. Next, after the cells were washed twice with PBS (−), 1 mL of PBS (−) was added and collected with a rubber policeman. PBS (-) 0.5mL was added to the pellet obtained by centrifugation, and the pellet was dissolved by ultrasonic crushing operation. Protein quantification was performed using the Lowry method {Lowry, O .; H. et al. , J .; Biol. Chem. , 193, 265-275 (1951)}. When measuring the amount of melanin per mg protein, 0.5 mL of 4N sodium hydroxide was added to the remaining cell disruption solution taken for protein quantification and reacted at 60 ° C. for 2 hours. D. 475 was measured, and melanin was determined from the calibration curve. The data was calculated as the amount of melanin per mg protein, the amount of melanin not added to the sample was taken as a control, and the value of the amount of melanin inhibition when the sample was added relative to the control was taken as the melanin inhibition rate.

  The results of these experiments are shown in Table 2. The above-ground extract of ogi showed an excellent inhibitory action on melanin production, and was more effective than the extract of ogi root.

Experimental Example 3 Use Test For 20 women (18 to 49 years old) using the lotion of Formulation Example 1, the cream of Formulation Example 2, the conventional lotion of Comparative Example 1 and the conventional cream of Comparative Example 2. A one month use test was conducted. After use, the improvement effect of skin wrinkles, tarmi, stains, buckwheat, etc. was determined by a questionnaire.

  These test results are shown in Tables 3 and 4. The external preparation for skin containing the extract of the upper part of the ogi showed excellent wrinkle, tarmi, stain and buckwheat improving actions. During the test period, there was no skin problem and there was no problem with safety. There was also no problem with the deterioration of the prescription ingredients.

When the usage test was similarly conducted for Formulation Examples 3 to 8, excellent wrinkle, tarmi, stain, buckwheat and other improving effects were shown.

Claims (2)

A whitening agent containing an extract of above-ground ogi as an active ingredient. A whitening cosmetic characterized by containing an extract from the upper part of ogi as a whitening agent.