JP4824181B2 - Topical skin preparation - Google Patents

Description

【００３３】
実験例２ 使用試験１
実施例１の化粧水、実施例２のクリーム、比較例１の従来の化粧水及び比較例２の従来のクリームを用いて、肌荒れに悩む女性３０人（２１〜４６才）を対象に１ヶ月間の使用試験を行った。使用後、肌荒れの改善効果をアンケートにより判定した。その結果を表２に示した。その結果、メシマコブの抽出物を含有することを特徴とする皮膚外用剤は優れた肌荒れの改善作用を示した。また、実施例１の化粧水と実施例２のクリームを使用して肌荒れが改善した全てのモニターは、肌のハリが改善され、しわが目立ちにくくなったと回答している。なお、試験期間中、皮膚トラブルは一人もなく、安全性においても問題なかった。また、処方成分の劣化についても問題なかった。
【００３４】
【表２】

【００３５】
実施例３の乳液、実施例４のゲル剤、実施例５の軟膏、実施例６のパック、実施例７のファンデーション、実施例８の浴用剤についても同様に使用試験を行ったところ、優れた肌荒れの改善、しわ予防の改善作用を示した。
【００３６】
【発明の効果】
以上示したように、本発明のメシマコブ子実体の抽出物は、優れたヒアルロニダーゼ阻害作用を有し、安定であった。さらに、メシマコブ子実体の抽出物を含有することを特徴とする皮膚外用剤は、安全性が高く、優れた肌荒れの改善、しわ予防作用を示した。[0001]
BACKGROUND OF THE INVENTION
The present invention relates to an external preparation for skin excellent in improving rough skin and preventing wrinkles .
[0002]
[Prior art]
There is skin inflammation caused by ultraviolet rays or the like as a cause of rough skin, and an external preparation for skin that subsides the inflammation and improves rough skin is desired. As one of the treatment methods, there is a method for suppressing the release of histamine, which is a inflammatory substance that occurs during inflammation. Conventionally, treatments for rough skin have been performed using steroids, indomethacin or the like.
[0003]
It is known that hyaluronidase is activated at the time of inflammation and lowers the molecular weight of hyaluronic acid, thereby reducing the elasticity of the skin and causing senile changes such as wrinkles.
[0004]
On the other hand, fruit body is generally used as a herbal medicine or health food having an antitumor action. Japanese Laid-Open Patent Publication No. 61-129113 is disclosed in which a mycelial culture is used as a bath preparation instead of fruiting bodies.
[0005]
[Problems to be solved by the invention]
Conventionally, steroids and the like used as histamine release inhibitors have a risk of side effects. Therefore, a natural product-derived skin external preparation having high safety and excellent effect is desired. In addition, there is a need for safe and stable hyaluronidase inhibitors.
[0006]
In view of the above, an external preparation for skin is desired for safety and stability, to improve rough skin and to prevent wrinkles .
[0007]
[Means for Solving the Problems]
In view of such circumstances, as a result of intensive studies, the present inventors have found that the extract of the fruit body of Meshimakobu has an excellent hyaluronidase activity inhibitory action and is also excellent in stability. Furthermore, the skin external preparation containing the extract was found to be safe and stable, and excellent in improving rough skin and preventing wrinkles , thereby completing the present invention.
[0008]
Meshimakobu used in the present invention (scientific name: phellinus linteus) is a basidiomycete of the genus Mushroom of the genus Tobacco mushroom, and its fruit bodies include natural products and cultivated products, and are obtained in markets such as Japan, Korea and China. Can do.
[0009]
The Meshimakobu extract used in the present invention is extracted from the fruit body of Meshimakobu. The extraction method is not particularly limited, and for example, it may be a heat extraction or a room temperature extraction.
[0010]
Examples of the solvent to be extracted include water, lower alcohols (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, 1-heptanol, 2-heptanol, etc.), liquid polyhydric alcohol (propylene) Glycol, glycerin, 1,3-butylene glycol, etc.), ketones (acetone, methyl ethyl ketone, etc.), acetonitrile, esters (ethyl acetate, butyl acetate, etc.), hydrocarbons (hexane, heptane, liquid paraffin, etc.), ethers (Ethyl ether, propyl ether, tetrahydrofuran, etc.). Preferred are polar solvents such as water, lower alcohols and liquid polyhydric alcohols, and particularly preferred are water, ethanol, propylene glycol and 1,3-butylene glycol. These solvents may be used alone or in combination of two or more.
[0011]
The extract of Meshimakobu may be used as it is, or may be used after concentration, dilution, filtration, or the like, if necessary. Further, the extracted solution may be subjected to a treatment such as concentration to dryness, spray drying, freeze drying, etc., and used as a dried product.
[0012]
In the external preparation for skin of the present invention, the above extract may be used as it is, and within the range not impairing the effect of the extract, oils and fats, waxes, hydrocarbons, fatty acids which are components used in the external preparation , Alcohols, esters, surfactants, metal soaps, pH adjusters, preservatives, fragrances, moisturizers, powders, UV absorbers, thickeners, pigments, antioxidants, whitening agents, chelating agents, etc. These components can be blended.
[0013]
The skin external preparation of the present invention can be used for any of cosmetics, quasi drugs, and pharmaceuticals. Examples of the dosage form include skin lotions, creams, emulsions, gels, aerosols, ointments, and patches. Examples thereof include agents, essences, packs, cleaning agents, bath preparations, foundations, dusting powders, lipsticks and the like applied to the skin.
[0014]
The amount of Meshimakobu extract used in the present invention is 0.0001% by weight or more, preferably 0.001 to 10% by weight in terms of solid matter, based on the total amount of the external preparation for skin of the present invention. If it is less than 0.0001% by weight, a sufficient effect is hardly expected. When the blending amount exceeds 10% by weight, the effect is hardly recognized and it is uneconomical. In addition, the addition method may be added in advance or during the production, and may be appropriately selected in consideration of workability.
[0015]
【Example】
Next, in order to describe the present invention in detail, examples of producing the extract used in the present invention, formulation examples and experimental examples of the present invention will be given as examples, but the present invention is not limited thereto. In the examples, the part of the amount is part by weight, and% is% by weight.
[0016]
Production Example 1 Hot water extract of Meshimakobu 400 ml of purified water was added to 20 g of dried Meshimakobu fruit body, extracted at 95-100 ° C. for 2 hours, filtered, and the filtrate was concentrated, freeze-dried and extracted with hot water. 0.86 g of product was obtained.
[0017]
Production Example 2 Mesima Kobu Ethanol Extract 2 g of ethanol was added to 200 g of Meshima Kobu fruit body, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain 2.2 g of an ethanol extract.
[0018]
Production Example 3 50% 1,3-Butylene Glycol Extract of Meshima Kobu Add 200 mL of purified water and 200 mL of 1,3-butylene glycol to 20 g of Meshima Kobu fruit body, extract at room temperature for 7 days, and filter to obtain 380 g of extract. It was.
[0019]
Example 1 Formulation for lotion 1. 1. Hot water extract of Messimacob (Production Example 1) 0.1 part 1,3-butylene glycol 8.0
3. Glycerin 2.0
4). Xanthan gum 0.02
5). Citric acid 0.01
6). Sodium citrate 0.1
7). Ethanol 5.0
8). Methyl paraoxybenzoate 0.1
9. Polyoxyethylene hydrogenated castor oil (40E.O.) 0.1
10. Perfume proper amount11. [Manufacturing method] Components 1 to 6 and 11 and components 7 to 10 are uniformly dissolved in purified water, and both are mixed and filtered to obtain a product.
[0020]
Comparative Example 1 Conventional Lotion A conventional lotion was obtained by replacing the hot water extract of Meshima Cobb with purified water in Example 1.
[0021]
Example 2 Cream formulation Messimacob ethanol extract (Production Example 2) 0.05 part Squalane 5.5
3. Olive oil 3.0
4). Stearic acid 2.0
5). Beeswax 2.0
6). Octyldodecyl myristate 3.5
7). Polyoxyethylene cetyl ether (20E.O.) 3.0
8). Behenyl alcohol 1.5
9. Glycerol monostearate2.5
10. Fragrance 0.1
11. Methyl paraoxybenzoate 0.2
12 Ethyl paraoxybenzoate 0.05
13. 1,3-butylene glycol 8.5
14 [Manufacturing method] Components 2 to 9 are heated and dissolved and mixed with purified water to a total amount of 100, and kept at 70 ° C to obtain an oil phase. Ingredients 1 and 11 to 14 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring. The component 10 is added at 45 ° C., and further cooled to 30 ° C. to obtain a product.
[0022]
Comparative Example 2 Conventional Cream In Example 2, a conventional cream was prepared by replacing the ethanol extract of Meshima Cobb with purified water.
[0023]
Example 3 Emulsion formulation 1. Hot water extract of Meshima Cobb (Production Example 1) 0.001 part Squalane 5.0
3. Olive oil 5.0
4). Jojoba oil 5.0
5). Cetanol 1.5
6). Glycerol monostearate 2.0
7). Polyoxyethylene cetyl ether (20E.O.) 3.0
8). Polyoxyethylene sorbitan monooleate (20E.O.) 2.0
9. Fragrance 0.1
10. Propylene glycol 1.0
11. Glycerin 2.0
12 Methyl paraoxybenzoate 0.2
13. [Manufacturing method] Components 2 to 8 are heated and dissolved and mixed with purified water to a total amount of 100, and kept at 70 ° C to obtain an oil phase. Ingredients 1 and 10-13 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring. The component 9 is added at 45 ° C., and further cooled to 30 ° C. to obtain a product.
[0024]
Example 4 Gel formulation formulation 1. 1.0 part of 50% 1,3-butylene glycol extract of Meshimakobu (Production Example 3) Ethanol 5.0
3. Methyl paraoxybenzoate 0.1
4). Polyoxyethylene hydrogenated castor oil (60 EO) 0.1
5). Perfume appropriate amount 6. 1,3-butylene glycol 5.0
7). Glycerin 5.0
8). Xanthan gum 0.1
9. Carboxyvinyl polymer 0.2
10. Potassium hydroxide 0.2
11. [Production method] Ingredients 2 to 5 and ingredients 1 and 6 to 11 are uniformly dissolved in purified water, and the two are mixed to obtain a product.
[0025]
Example 5 Ointment formulation Formulation amount 1. Hot water extract of Messimacob (Production Example 1) 0.01 part Messimacob 50% 1,3-butylene glycol extract (Production Example 3) 0.5
3. Polyoxyethylene cetyl ether (30E.O.) 2.0
4). Glycerol monostearate 10.0
5). Liquid paraffin 5.0
6). Cetanol 6.0
7). Methyl paraoxybenzoate 0.1
8). Propylene glycol 10.0
9. [Production Method] Components 3 to 6 are heated and dissolved and mixed with purified water to a total amount of 100, and kept at 70 ° C. to obtain an oil phase. Ingredients 1, 2, and 7-9 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled to 30 ° C. with stirring to obtain a product.
[0026]
Example 6 Pack Formulation Amount 1. Hot water extract of Messimacob (Production Example 1) 0.1 part Ethima extract of Meshimakobu (Production Example 2) 0.1
3. Polyvinyl alcohol 12.0
4). Ethanol 5.0
5). 1,3-butylene glycol 8.0
6). Methyl paraoxybenzoate 0.2
7). Polyoxyethylene hydrogenated castor oil (20 EO) 0.5
8). Citric acid 0.1
9. Sodium citrate 0.3
10. Perfume proper amount11. [Production Method] Components 1 to 11 are uniformly dissolved in purified water to make a total amount of 100 to obtain a product.
[0027]
Example 7 Foundation Formulation Blending amount 1. Ethanol extract of Meshimakobu (Production Example 2) 1.0 part Stearic acid 2.4
3. Polyoxyethylene sorbitan monostearate (20EO) 1.0
4). Polyoxyethylene cetyl ether (20E.O.) 2.0
5). Cetanol 1.0
6). Liquid lanolin 2.0
7). Liquid paraffin 3.0
8). Isopropyl myristate 6.5
9. Butyl paraoxybenzoate 0.1
10. Sodium carboxymethylcellulose 0.1
11. Bentonite 0.5
12 Propylene glycol 4.0
13. Triethanolamine 1.1
14 Methyl paraoxybenzoate 0.2
15. Titanium dioxide 8.0
16. Talc 4.0
17. Bengala 1.0
18. Yellow iron oxide 2.0
19. Perfume proper amount20. [Manufacturing method] Components 2 to 9 are heated and dissolved in purified water to make the total amount 100, and kept at 80 ° C to obtain an oil phase. Swell component 10 well with component 20, then add components 1 and 11-14 and mix uniformly. To this, components 15 to 18 pulverized and mixed with a pulverizer are added, and the mixture is stirred with a homomixer and kept at 75 ° C. to obtain an aqueous phase. The oily phase is added to the aqueous phase with stirring, cooled, and component 19 is added at 45 ° C, and cooled to 30 ° C with stirring to give a product.
[0028]
Example 8 Bath preparation formulation Meshimakobu hot water extract (Production Example 1) 5.0 parts Sodium bicarbonate 50.0
3. Yellow No. 202 (1) Appropriate amount 4. Perfume appropriate amount 5. [Manufacturing method] Ingredients 1 to 5 are mixed uniformly with sodium sulfate to make a product.
[0029]
Next, experimental examples will be given to explain the effects of the present invention in detail.
[0030]
Experimental Example 1 Hyaluronidase inhibitory action (anti-aging action)
About the extract of manufacture example 1,2,3, the hyaluronidase inhibitory effect was measured according to the method [Food Hygiene Journal, 31, 3 (1990)] which applied Morgan-Elson method. That is, 175 mL of 0.1 M acetate buffer (pH 4.0) was added to the sample solution, the enzyme activity of hyaluronidase was 400 U / mL, the concentration of hyaluronic acid was 0.4 mg / mL, and the compound of activator 48/80 was added. After adjusting the total amount to 500 mL so that it might become 0.06 mg / mL, hyaluronidase reaction was implemented at 37 degreeC for 40 minute (s). After the reaction, p-dimethylaminobenzaldehyde reagent was added to cause color development, and the absorbance at 585 nm was measured. Moreover, the inhibitory action of each sample was calculated by the inhibition rate calculated | required from the following formula. For the control, purified water was used instead of the sample, and purified water was used instead of hyaluronidase as a blank.
Inhibition rate (%) = [1- (C−D) / (A−B)] × 100
A: Absorbance at 585 nm of control (OD 585)
B: O. D. 585
C: Sample O.D. D. 585
D: Sample blank O.D. D. 585
[0031]
The results of these experiments are shown in Table 1 . As a result, the Meshimakobu extract of Production Examples 1 and 2 showed an excellent hyaluronidase inhibitory action.
[0032]
[Table 1]

[0033]
Experiment 2 Use test 1
One month for 30 women (21 to 46 years old) suffering from rough skin using the lotion of Example 1, the cream of Example 2, the conventional lotion of Comparative Example 1 and the conventional cream of Comparative Example 2 A usage test was conducted. After use, the improvement effect of rough skin was judged by a questionnaire. The results are shown in Table 2 . As a result, an external preparation for skin characterized by containing an extract of Meshima Cobb showed an excellent effect of improving rough skin. Moreover, all the monitors which improved skin roughness using the lotion of Example 1 and the cream of Example 2 answered that the firmness of the skin was improved and wrinkles became less noticeable. During the test period, there was no skin problem and there was no problem with safety. There was also no problem with the deterioration of the prescription ingredients.
[0034]
[Table 2]

[0035]
The same tests were conducted on the emulsion of Example 3, the gel of Example 4, the ointment of Example 5, the pack of Example 6, the foundation of Example 7, and the bath preparation of Example 8, and the results were excellent. It showed improvement of rough skin and wrinkle prevention .
[0036]
【The invention's effect】
As described above, the extract of the fruit body of the present invention of the present invention has an excellent hyaluronidase inhibitory action and is stable. Furthermore, the skin external preparation characterized by containing an extract of Meshimakobu fruit body has high safety, and has excellent skin roughening improvement and wrinkle prevention action .

Claims (1)

A hyaluronidase activity inhibitor comprising an extract of fruit body of Meshimakobu.