JP3930179B2 - Topical skin preparation - Google Patents

Description

【００３２】
実験例２ 使用試験
実施例１の化粧水、実施例２のクリーム、比較例１の従来の化粧水及び比較例２の従来のクリームを用いて、シミ、ソバカスに悩む女性１５人（３０〜４５才）を対象に１ヶ月間の使用試験を行った。使用後に、シミ、ソバカスの改善に関するアンケート調査をすることにより美白作用を判定した。その結果、トリコーパス・ゼイラニクスの抽出物を含有する皮膚外用剤は優れた美白作用を示した（表２）。なお、試験期間中に皮膚トラブルは一人もなく、安全性においても問題なかった。
【００３３】
【表２】

【００３４】
実施例３の乳液、実施例４のゲル剤、実施例５の軟膏、実施例６のパック、実施例７のファンデーション及び実施例８の浴用剤についても使用試験を行ったところ、優れた美白作用を示した。また、安全性においても問題なかった。
【００３５】
【発明の効果】
以上のことから、本発明のトリコーパス・ゼイラニクスの抽出物は、優れた美白作用を示した。また、その抽出物を含有することを特徴とする皮膚外用剤は安全性が高く、優れた美白作用を示した。[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a skin external preparation excellent in whitening action.
[0002]
[Prior art]
In general, pigmentation of the skin seen in spots, buckwheat, sunburn, etc., is caused by excessive melanin pigment formation by melanin-producing cells present in the skin due to hormonal abnormalities or stimulation of ultraviolet rays, which deposits in the skin. Is considered to be the cause. As one method for preventing such pigmentation, a method for suppressing excessive production of melanin is known. Conventionally, hydroquinone or the like has been applied externally for the treatment of pigmentation.
[0003]
However, chemically synthesized products such as hydroquinone used as a whitening agent have a risk of side effects, and are highly safe and highly effective.
[0004]
[Problems to be solved by the invention]
From the above, it is an object to provide a skin external preparation that is safe against skin and has an excellent whitening effect.
[Means for Solving the Problems]
[0005]
In view of such circumstances, as a result of intensive studies, the present inventors have found that the extract of Trichopus zeiranics has an excellent melanin production inhibitory action, and further, the skin external preparation containing the extract is excellent. The present invention was completed because of the whitening effect.
[0006]
That is, this invention is an external preparation for skin containing the extract of Trichopus zeiranicus.
[0007]
Trichopus zeylanicus used in the present invention belongs to the genus Trichopus genus Trichopus, and is a perennial herb that is distributed mainly in southern India and Sri Lanka. Locally used mainly as a nourishing tonic.
[0008]
The extract of Trichopus zeaninics used in the present invention is obtained by extracting from a part of a plant body such as trichomes zeiranicus leaves, stems, flowers, berries, roots or the whole plant. Preferably, one obtained by extraction from one of leaves or stems or a mixture of both is good. The preparation method is not particularly limited, and for example, it may be extracted by heating or may be extracted at room temperature. Examples of the solvent to be extracted include water, water-soluble solvents and water-insoluble solvents. Examples of water-soluble solvents include lower monohydric alcohols (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), liquid polyhydric alcohols (1,3-butylene glycol, propylene glycol, etc.), ketones (acetone, methyl ethyl ketone, etc.), acetonitrile and the like. Non-water-soluble solvents include esters (ethyl acetate) , Butyl acetate, etc.), hydrocarbons (hexane, heptane, liquid paraffin, etc.), ethers (ethyl ether, tetrahydrofuran, propyl ether, etc.). Water and a water-soluble solvent are preferable, and water, ethanol, 1,3-butylene glycol or propylene glycol is particularly preferable. These solvents may be used alone or in combination of two or more.
[0009]
In addition, the extract of Trichopus zeiranicus may be used as it is in the extracted solution, or may be used after processing such as concentration, dilution, and filtration as necessary. Further, the extracted solution may be subjected to a treatment such as concentration to dryness, spray drying, freeze drying, etc., and used as a dried product.
[0010]
Oils, waxes, hydrocarbons, fatty acids, alcohols that are components used in normal skin external preparations, as long as the effects of the extract of Trichopus zeiranicus are not impaired in the external preparations of the present invention Contains ingredients such as esters, surfactants, metal soaps, pH adjusters, preservatives, fragrances, moisturizers, powders, UV absorbers, thickeners, dyes, antioxidants, cosmetic ingredients, and chelating agents. can do.
[0011]
The skin external preparation of the present invention can be used for any of pharmaceuticals, quasi drugs, and cosmetics. Examples of the dosage form include skin lotions, creams, emulsions, gels, aerosols, ointments, poultices, Examples include pastes, plaster agents, essences, packs, cleaning agents, bath preparations, foundations, dusting powders, and lipsticks that are applied to the skin.
[0012]
The blending amount of the extract of Trichopus zeiranicus used in the present invention is 0.00001% by weight or more, preferably 0.00005 to 10% by weight, more preferably, in terms of dry solids, based on the total amount of the external preparation for skin of the present invention. 0.0005 to 10% by weight is good. If it is less than 0.00001% by weight, a sufficient effect cannot be expected. When it exceeds 10% by weight, the effect is not enhanced and it is uneconomical. In addition, the addition method may be added in advance or during the production, and may be appropriately selected in consideration of workability.
[0013]
【Example】
Next, examples are given to describe the present invention in detail, but the present invention is not limited to these examples. In the examples, the part of the amount is part by weight, and% is% by weight.
[0014]
Production Example 1 Trichopus zeiranix hot water extract Trichopus zeiranicus whole plant dried product 400ml of purified water was added to 20g, extracted at 95-100 ° C for 2 hours, filtered, and the filtrate was concentrated. Then, it was freeze-dried to obtain 2.2 g of a hot water extract of Trichopus zeiranicus.
[0015]
Production Example 2 Trichopus zeiranicus ethanol extract 1 g of ethanol was added to 200 g of dried trichomes zeiranicus whole plant, stirred for 2 hours at room temperature, extracted, filtered, and the filtrate was concentrated and frozen. Drying gave 3.8 g of ethanol extract of Trichopus zeiranicus.
[0016]
Production Example 3 Trichopus ZEIRANIX 50% ethanol extract Trichopus ZEIRANIX whole plant dry product 20 g of 1 L of 50% ethanol aqueous solution was added to the product, stirred at room temperature for 2 hours, extracted, filtered and filtrated. Was concentrated and freeze-dried to obtain 2.6 g of a 50% ethanol extract of Trichopus zeiranicus.
[0017]
Production Example 4 1,3-butylene glycol extract of Trichopus zeiranicus After adding 500 ml of 1,3-butyleneglycol to 100 g of dried trichomes zeiranicus leaves and stems and soaking at room temperature for 7 days, extraction And filtered to obtain 470 g of 1,3-butylene glycol extract of Trichopus zeiranicus.
[0018]
Example 1 Lotion 1
Formulation amount
1. Tricorpus zealanix hot water extract (Production Example 1) 1.0 part
2. 1,3-Butylene glycol 8.0
3. Glycerin 2.0
4. Xanthan gum 0.02
5. Citric acid 0.01
6. Sodium citrate 0.1
7. Ethanol 5.0
8. Methyl paraoxybenzoate 0.1
9. Polyoxyethylene hydrogenated castor oil (40E.O.) 0.1
10. Perfume appropriate amount
11. Make the total amount 100 with purified water [Manufacturing method] Components 1 to 6 and 11 and components 7 to 10 are uniformly dissolved, mixed and filtered to obtain a product.
[0019]
Comparative Example 1 Conventional lotion In Example 1 of the conventional lotion, the hot water extract of Trichopus zeiranicus was replaced with purified water.
[0020]
Example 2 Cream formulation
1. Ethanol extract of Trichopus zeiranicus (Production Example 2) 0.05 part
2. Squalane 5.5
3. Olive oil 3.0
4. Stearic acid 2.0
5. Beeswax 2.0
6. Octyldodecyl myristate 3.5
7. Polyoxyethylene cetyl ether (20E.O.) 3.0
8. Behenyl alcohol 1.5
9. Glycerol monostearate 2.5
10. Fragrance 0.1
11. 1,3-Butylene glycol 8.5
12. Ethyl paraoxybenzoate 0.05
13. Methyl paraoxybenzoate 0.2
14. Make the total amount to 100 with purified water [Production method] Components 1 to 9 are heated and dissolved and mixed, and kept at 70 ° C to obtain an oil phase. Ingredients 11-14 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. Add the water phase to the oil phase, emulsify, cool with stirring, add component 10 at 45 ° C, and cool to 30 ° C to make the product.
[0021]
Comparative Example 2 A conventional cream obtained by replacing the ethanol extract of Trichopus zeiranicus with purified water in Example 2 of the conventional cream was used.
[0022]
Example 3 Emulsion formulation
1. Tricopus Zeillanicus 50% ethanol extract (Production Example 3) 1.0 part
2. Squalane 5.0
3. Olive oil 5.0
4. Jojoba oil 5.0
5. Cetanol 1.5
6. Glycerol monostearate 2.0
7. Polyoxyethylene cetyl ether (20E.O.) 3.0
8. Polyoxyethylene sorbitan monooleate (20E.O.) 2.0
9. Fragrance 0.1
10. Propylene glycol 1.0
11. Glycerin 2.0
12. Methyl paraoxybenzoate 0.2
13. Make the total amount to 100 with purified water [Production Method] Components 2 to 8 are dissolved by heating and mixed, and kept at 70 ° C to obtain an oil phase. Ingredients 1 and 10-13 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. Add the water phase to the oil phase, emulsify, cool with stirring, add component 9 at 45 ° C, and cool to 30 ° C to make the product.
[0023]
Example 4 Gel formulation formulation
1. Trichopus Zeillanicus 1,3-butylene glycol extract (Production Example 4) 2.0 parts
2. Ethanol 5.0
3. Methyl paraoxybenzoate 0.1
4. Polyoxyethylene hydrogenated castor oil (60E.O.) 0.1
5. Perfume appropriate amount
6. 1,3-Butylene glycol 5.0
7. Glycerin 5.0
8. Xanthan gum 0.1
9. Carboxyvinyl polymer 0.2
10. Potassium hydroxide 0.2
11. Make the total amount 100 with purified water [Production method] Ingredients 1-5 and 6-11 are uniformly dissolved and mixed to make a product.
[0024]
Example 5 Ointment Formulation Amount
1. Tricorpus zealanix hot water extract (Production Example 1) 1.0 part
2. Ethanol extract of Trichopus zeiranicus (Production Example 2) 1.0
3. Polyoxyethylene cetyl ether (30E.O.) 2.0
4. Glycerol monostearate 10.0
5. Liquid paraffin 5.0
6. Cetanol 6.0
7. Methyl paraoxybenzoate 0.1
8. Propylene glycol 10.0
9. Make the total amount to 100 with purified water [Production method] Components 2 to 6 are heated and dissolved and mixed, and kept at 70 ° C to obtain an oil phase. Ingredients 1 and 7-9 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. Add the water phase to the oil phase, emulsify, and cool to 30 ° C with stirring to make the product.
[0025]
Example 6 Pack formulation
1. Hot water extract of Trichopus zeiranicus (Production Example 1) 0.1 part
2. 50% ethanol extract of Trichopus zeiranicus (Production Example 3) 0.1
3. Polyvinyl alcohol 12.0
4. Ethanol 5.0
5. 1,3-Butylene glycol 8.0
6. Methyl paraoxybenzoate 0.2
7. Polyoxyethylene hydrogenated castor oil (20E.O.) 0.5
8. Citric acid 0.1
9. Sodium citrate 0.3
10. Perfume appropriate amount
11. Make the total amount to 100 with purified water. [Production method] Ingredients 1 to 11 are uniformly dissolved to make a product.
[0026]
Example 7 Foundation formulation
1. Tricorpus zealanix hot water extract (Production Example 1) 1.0 part
2. Stearic acid 2.4
3. Polyoxyethylene sorbitan monostearate (20E.O.) 1.0
4. Polyoxyethylene cetyl ether (20E.O.) 2.0
5. Cetanol 1.0
6. Liquid lanolin 2.0
7. Liquid paraffin 3.0
8. Isopropyl myristate 6.5
9. Butyl paraoxybenzoate 0.1
10. Carboxymethylcellulose sodium 0.1
11. Bentonite 0.5
12. Propylene glycol 4.0
13. Triethanolamine 1.1
14. Methyl paraoxybenzoate 0.2
15. Titanium dioxide 8.0
16. Talc 4.0
17. Bengala 1.0
18. Yellow iron oxide 2.0
19. Perfume
20. Make the total amount to 100 with purified water. [Production method] Components 2 to 9 are heated and dissolved and kept at 80 ° C to form an oil phase. Swell component 10 well into component 20, then add components 1 and 11-14 and mix uniformly. To this, components 15 to 18 pulverized and mixed with a pulverizer are added, and the mixture is stirred with a homomixer and kept at 75 ° C. to obtain an aqueous phase. Add the oil phase to this aqueous phase with stirring, cool, add component 19 at 45 ° C, and cool to 30 ° C with stirring to make the product.
[0027]
Example 8 Formulation for bath preparation
1. Hot water extract of Trichopus zeiranicus (Production Example 1) 5.0 parts
2. Sodium bicarbonate 50.0
3. Yellow 202 No.
4. Perfume appropriate amount
5. Make the total amount 100 with sodium sulfate. [Production method] Ingredients 1 to 5 are uniformly mixed to make a product.
[0028]
Next, experimental examples will be given to explain the effects of the present invention in detail.
[0029]
Experimental example 1 Melanin production inhibition test using B16 mouse melanoma 3 × 10 ⁴ cells of melanoma in the logarithmic growth phase were seeded in a φ60 mm dish, and various concentrations of Trichopus zeiranix hot water extract, ethanol extract or positive control Eagles' MEM containing 10% ascorbic acid (containing 10% fetal bovine serum) was added and cultured under conditions of 37 ° C. and 5% CO ₂ . After 5 days of culture, the cells were detached from the dish, and the cells were sonicated, then treated with 2N NaOH at 60 ° C. for 2 hours, and OD475 nm was measured with a spectrophotometer. In addition, the cell lysate after sonication is quantified with Lowry's method (J. Biol. Chem., 193, 265-275, 1951), and the amount of melanin per protein is compared. It was used as an index.
[0030]
The test results are shown in Table 1. As a result, it was confirmed that the extract of Trichopus zeiranicus has an excellent melanin production inhibitory action.
[0031]
[Table 1]

[0032]
Experimental Example 2 Use test 15 skin lotion of Example 1, the cream of Example 2, the conventional skin lotion of Comparative Example 1 and the conventional cream of Comparative Example 2, 15 women (30-45) suffering from spots and freckles A one-month use test was conducted. After use, the whitening effect was determined by conducting a questionnaire survey on the improvement of spots and freckles. As a result, the external preparation for skin containing the extract of Trichopus zeiranix exhibited an excellent whitening effect (Table 2). There was no skin problem during the test period, and there was no problem in safety.
[0033]
[Table 2]

[0034]
When a use test was conducted on the emulsion of Example 3, the gel of Example 4, the ointment of Example 5, the pack of Example 6, the foundation of Example 7, and the bath preparation of Example 8, an excellent whitening effect was obtained. showed that. There was no problem in safety.
[0035]
【The invention's effect】
From the above, the extract of Trichopus zeiranicus of the present invention showed an excellent whitening effect. Moreover, the skin external preparation characterized by containing the extract was highly safe and exhibited an excellent whitening effect.

Claims (2)

An external skin preparation characterized by containing an extract of Trichopus zeiranicus. The skin external preparation according to claim 1, wherein the extract is water and / or a water-soluble solvent extract.